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General Information
Symbol
Dmel\CycE
Species
D. melanogaster
Name
Cyclin E
Annotation Symbol
CG3938
Feature Type
FlyBase ID
FBgn0010382
Gene Model Status
Stock Availability
Gene Summary
Essential for the control of the cell cycle at the G1/S (start) transition. Targeted by archipelago for degradation by the SFC ubiquitin ligase complex. (UniProt, P54733)
Contribute a Gene Snapshot for this gene.
Also Known As

DmcycE, l(2)br37, l(2)35Dd, fond, Cyc E

Key Links
Genomic Location
Cytogenetic map
Sequence location
2L:15,727,581..15,748,150 [-]
Recombination map
2-51
RefSeq locus
NT_033779 REGION:15727581..15748150
Sequence
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
Function
GO Summary Ribbons
Gene Ontology (GO) Annotations (28 terms)
Molecular Function (2 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
inferred from physical interaction with FLYBASE:mi; FB:FBgn0261786
inferred from physical interaction with FLYBASE:brm; FB:FBgn0000212
inferred from physical interaction with FLYBASE:Snr1; FB:FBgn0011715
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
inferred from biological aspect of ancestor with PANTHER:PTN000019791
(assigned by GO_Central )
Biological Process (21 terms)
Terms Based on Experimental Evidence (19 terms)
CV Term
Evidence
References
inferred from mutant phenotype
inferred from direct assay
(assigned by UniProt )
inferred from mutant phenotype
inferred from mutant phenotype
inferred from direct assay
inferred from expression pattern
(assigned by UniProt )
inferred from expression pattern
(assigned by UniProt )
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from genetic interaction with FLYBASE:CycA; FB:FBgn0000404
inferred from genetic interaction with FLYBASE:fzr; FB:FBgn0262699
inferred from direct assay
inferred from mutant phenotype
inferred from direct assay
inferred from high throughput mutant phenotype
Terms Based on Predictions or Assertions (3 terms)
CV Term
Evidence
References
inferred from biological aspect of ancestor with PANTHER:PTN000808611
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN000019791
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN000019791
(assigned by GO_Central )
Cellular Component (5 terms)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
located_in nucleus
inferred from direct assay
inferred from direct assay
(assigned by UniProt )
Terms Based on Predictions or Assertions (5 terms)
CV Term
Evidence
References
is_active_in centrosome
inferred from biological aspect of ancestor with PANTHER:PTN002468700
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN000808611
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN000019791
(assigned by GO_Central )
is_active_in cytoplasm
inferred from biological aspect of ancestor with PANTHER:PTN000019791
(assigned by GO_Central )
is_active_in nucleus
inferred from biological aspect of ancestor with PANTHER:PTN000019791
(assigned by GO_Central )
Gene Group (FlyBase)
Protein Family (UniProt)
Belongs to the cyclin family. Cyclin E subfamily. (P54733)
Summaries
Pathway (FlyBase)
Positive Regulators of JAK-STAT Signaling Pathway -
Positive regulators of JAK-STAT signaling up-regulate the pathway, enhancing transcriptional control by Stat92E.
Protein Function (UniProtKB)
Essential for the control of the cell cycle at the G1/S (start) transition. Targeted by archipelago for degradation by the SFC ubiquitin ligase complex.
(UniProt, P54733)
Summary (Interactive Fly)
Gene Model and Products
Number of Transcripts
6
Number of Unique Polypeptides
3

Please see the JBrowse view of Dmel\CycE for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model

Insertion of transposon immediately 3' of gene may force use of alternative polyA addition sites (see cDNAs derived from sequenced strain vs. X75026).

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.52

Sequence Ontology: Class of Gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
FBtr0080774
3653
709
FBtr0080777
3856
602
FBtr0080775
3491
709
FBtr0080776
3946
709
FBtr0080773
3703
709
FBtr0305561
3363
712
Additional Transcript Data and Comments
Reported size (kB)

3.9 (compiled cDNA)

Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
FBpp0080332
77.2
709
4.43
FBpp0080335
66.7
602
4.49
FBpp0080333
77.2
709
4.43
FBpp0080334
77.2
709
4.43
FBpp0080331
77.2
709
4.43
FBpp0294012
77.7
712
4.43
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

709 aa isoforms: CycE-PA, CycE-PC, CycE-PD, CycE-PE
Additional Polypeptide Data and Comments
Reported size (kDa)
Comments

CycE protein is the major partner of cdc2c

protein. CycE and cdc2c kinase activity are present throughout the

cell cycle.

External Data
Subunit Structure (UniProtKB)

Interacts with a member of the CDK2/CDK protein kinases to form a serine/threonine kinase holoenzyme complex (PubMed:11565033). The cyclin subunit imparts substrate specificity to the complex (PubMed:11565033). Interacts (via C-terminus) with Z600 (via C-terminus) (PubMed:17431409).

(UniProt, P54733)
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Nucleotide / Polypeptide Records
 
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\CycE using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
organism

Comment: maternally deposited

organism | posterior

Comment: reference states 1-2 hr AEL

dorsal ectoderm anlage

Comment: anlage in statu nascendi

mesoderm anlage

Comment: anlage in statu nascendi

ventral ectoderm anlage

Comment: anlage in statu nascendi

antennal anlage in statu nascendi

Comment: reported as procephalic ectoderm anlage in statu nascendi

dorsal head epidermis anlage in statu nascendi

Comment: reported as procephalic ectoderm anlage in statu nascendi

visual anlage in statu nascendi

Comment: reported as procephalic ectoderm anlage in statu nascendi

antennal primordium

Comment: reported as procephalic ectoderm primordium

central brain primordium

Comment: reported as procephalic ectoderm primordium

visual primordium

Comment: reported as procephalic ectoderm primordium

dorsal head epidermis primordium

Comment: reported as procephalic ectoderm primordium

lateral head epidermis primordium

Comment: reported as procephalic ectoderm primordium

ventral head epidermis primordium

Comment: reported as procephalic ectoderm primordium

Additional Descriptive Data

At embryonic stages 8-9 CycE transcript is expressed in three columns of neuroblasts per segment, forming a series of segmentally repeated stripes.

CycE transcripts disappear from the cells of the dorsal epidermis just before they go through their final division (mitosis 16). They are present in the region of the dorsal epidermis that goes through an extra division and disappear during additional cycle 17. CycE transcripts are present in the proliferating cells of the CNS and PNS. They are also observed in endoreplicating tissues.

Type 1 CycE transcripts are first detected after cellularization in a striped pattern along the anterior-posterior axis of the embryo. During gastrulation, high levels of CycE transcripts are seen in the neurectoderm. Expression is seen in the primary neuroblasts as they delaminate from the ectoderm. During germ band extension, a dynamic pattern of staining is seen in the CNS and PNS. Later expression occurs in cells of the ventral nerve cord and in the proliferative centers of the brain lobes. This pattern is consistent with the pattern of proliferation of neural cells in the CNS.

Type 2 CycE transcripts are expressed at the earliest stage where embryos are undergoing rapid nuclear divisions in a syncytium (0-2hrs). At the stage of pole cell formation, transcripts are more concentrated at the posterior pole where the pole cells are forming.

Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
cell | subset
nucleus

Comment: interphase and telophase nuclei of mitotic cells

Additional Descriptive Data

Expression intensity of CycE varies as the cell cycle progresses, with greatest intensity found in the latter part of phase G2.

CycE protein is present throughout the early embryonic cycles. It is predominantly nuclear during interphase and becomes dispersed in the cytoplasm during mitosis. Two types of CycE protein are observed by immunoblotting. The form with the lower mobility is maximal during mitosis. The higher mobility form is abundant in G2 and is nearly absent during M and early S phases.

High levels of CycE protein are detected in histoblasts in third instar larvae before histoblasts initiate divisions. Stored CycE declines progressively during the first cell cycles and is depleted by the third prepupal cycle a 4hr APF.

CycE protein is expressed at varying levels (suggesting cycling) in both nurse cells and follicle cells. Within individual egg chambers, nurse cell nuclei with high, intermediate or low (to undetectable) levels of CycE protein are observed. Levels are always high in the germinal vesicle and increase as egg chambers develop.

CycE protein is present in embryos in mitotically proliferating and endoreplicating cells. In larval optic lobes, the pattern of CycE protein expression is similar to the pattern of S phases. It is present in the outer optic anlage, the inner optic anlage, and in a band corresponding to the S phase lamina precursor cells. It is absent in G1 phase lamina precursor cells. It is also present in a region of the lamina where only a portion of the cells are in S phase. In the eye imaginal disc, CycE protein is present in a subset of the asynchronously proliferating cells and in a band of cells just posterior to the morphogenetic furrow that are in S phase but not in G1 cells within and anterior to the furrow. In summary, CycE protein is absent in G1 phase cells but appears at the onset of S phase in proliferating cells of the larval optic lobe and eye imaginal disc.

Marker for
 
Subcellular Localization
CV Term
Evidence
References
located_in nucleus
inferred from direct assay
inferred from direct assay
(assigned by UniProt )
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\CycE in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
EMBL-EBI Single Cell Expression Atlas
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyExpress - Embryonic expression images (BDGP data)
  • Stages(s) 4-6
  • Stages(s) 7-8
  • Stages(s) 9-10
  • Stages(s) 11-12
  • Stages(s) 13-16
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 54 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 49 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of CycE
Transgenic constructs containing regulatory region of CycE
Aberrations (Deficiencies and Duplications) ( 175 )
Inferred from experimentation ( 175 )
Gene disrupted in
Gene not disrupted in
Inferred from location ( 0 )
Alleles Representing Disease-Implicated Variants
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
Sterility
Allele
Other Phenotypes
Allele
Phenotype manifest in
Allele
anterior midgut proper primordium & mitotic cell cycle
follicle cell & nucleus | somatic clone, with Scer\GAL4Act5C.PP
G1 phase & external sensory organ precursor cell IIIb, with Scer\GAL4neur-P72
glial cell & eye disc | ectopic, with Scer\GAL4bi-omb-Gal4
heterochromatin & nurse cell
larval salivary gland & embryo & nucleus, with Scer\GAL4F4
macrochaeta & thorax
neuron & embryonic abdomen | ectopic, with Scer\GAL4sca-537.4
nurse cell & nucleus
Orthologs
Human Orthologs (via DIOPT v8.0)
Homo sapiens (Human) (21)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
11 of 15
Yes
Yes
1  
9 of 15
No
Yes
1  
3 of 15
No
No
3 of 15
No
No
3 of 15
No
Yes
2 of 15
No
No
2 of 15
No
No
1  
2 of 15
No
No
1  
2 of 15
No
No
2 of 15
No
Yes
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
1  
2 of 15
No
No
1  
2 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
Model Organism Orthologs (via DIOPT v8.0)
Mus musculus (laboratory mouse) (17)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
12 of 15
Yes
Yes
10 of 15
No
Yes
3 of 15
No
No
3 of 15
No
No
3 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2 of 15
No
Yes
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
1 of 15
No
No
1  
1 of 15
No
No
1 of 15
No
No
Rattus norvegicus (Norway rat) (19)
11 of 13
Yes
Yes
9 of 13
No
Yes
3 of 13
No
No
3 of 13
No
No
3 of 13
No
No
2 of 13
No
No
2 of 13
No
No
2 of 13
No
Yes
2 of 13
No
No
2 of 13
No
No
2 of 13
No
No
2 of 13
No
No
2 of 13
No
No
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
Xenopus tropicalis (Western clawed frog) (22)
10 of 12
Yes
Yes
9 of 12
No
Yes
3 of 12
No
No
3 of 12
No
No
3 of 12
No
No
3 of 12
No
No
2 of 12
No
No
2 of 12
No
No
2 of 12
No
Yes
2 of 12
No
No
2 of 12
No
No
2 of 12
No
No
2 of 12
No
No
2 of 12
No
No
2 of 12
No
No
2 of 12
No
Yes
1 of 12
No
No
1 of 12
No
No
1 of 12
No
No
1 of 12
No
No
1 of 12
No
Yes
1 of 12
No
Yes
Danio rerio (Zebrafish) (22)
12 of 15
Yes
Yes
7 of 15
No
Yes
3 of 15
No
No
3 of 15
No
No
3 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
Yes
1 of 15
No
No
Caenorhabditis elegans (Nematode, roundworm) (16)
12 of 15
Yes
Yes
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
Arabidopsis thaliana (thale-cress) (23)
2 of 9
Yes
Yes
2 of 9
Yes
No
2 of 9
Yes
No
2 of 9
Yes
No
2 of 9
Yes
No
2 of 9
Yes
No
2 of 9
Yes
No
2 of 9
Yes
No
2 of 9
Yes
No
2 of 9
Yes
No
2 of 9
Yes
No
1 of 9
No
No
1 of 9
No
No
1 of 9
No
No
1 of 9
No
No
1 of 9
No
No
1 of 9
No
No
1 of 9
No
No
1 of 9
No
No
1 of 9
No
No
1 of 9
No
No
1 of 9
No
No
1 of 9
No
Yes
Saccharomyces cerevisiae (Brewer's yeast) (9)
3 of 15
Yes
No
3 of 15
Yes
No
3 of 15
Yes
No
3 of 15
Yes
No
3 of 15
Yes
Yes
3 of 15
Yes
Yes
2 of 15
No
No
2 of 15
No
No
2 of 15
No
Yes
Schizosaccharomyces pombe (Fission yeast) (5)
1 of 12
Yes
No
1 of 12
Yes
No
1 of 12
Yes
No
1 of 12
Yes
Yes
1 of 12
Yes
No
Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG0919060P )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila suzukii
Spotted wing Drosophila
Drosophila simulans
Drosophila sechellia
Drosophila erecta
Drosophila yakuba
Drosophila ananassae
Drosophila pseudoobscura pseudoobscura
Drosophila persimilis
Drosophila willistoni
Drosophila virilis
Drosophila mojavensis
Drosophila grimshawi
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091505DS )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Glossina morsitans
Tsetse fly
Lucilia cuprina
Australian sheep blowfly
Mayetiola destructor
Hessian fly
Aedes aegypti
Yellow fever mosquito
Anopheles darlingi
American malaria mosquito
Anopheles gambiae
Malaria mosquito
Culex quinquefasciatus
Southern house mosquito
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W07JT )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Danaus plexippus
Monarch butterfly
Heliconius melpomene
Postman butterfly
Apis florea
Little honeybee
Apis mellifera
Western honey bee
Bombus impatiens
Common eastern bumble bee
Bombus terrestris
Buff-tailed bumblebee
Linepithema humile
Argentine ant
Megachile rotundata
Alfalfa leafcutting bee
Nasonia vitripennis
Parasitic wasp
Tribolium castaneum
Red flour beetle
Pediculus humanus
Human body louse
Rhodnius prolixus
Kissing bug
Cimex lectularius
Bed bug
Cimex lectularius
Bed bug
Acyrthosiphon pisum
Pea aphid
Zootermopsis nevadensis
Nevada dampwood termite
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X03VF )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strigamia maritima
European centipede
Ixodes scapularis
Black-legged tick
Stegodyphus mimosarum
African social velvet spider
Tetranychus urticae
Two-spotted spider mite
Daphnia pulex
Water flea
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G0DAW )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strongylocentrotus purpuratus
Purple sea urchin
Ciona intestinalis
Vase tunicate
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Paralogs
Paralogs (via DIOPT v8.0)
Drosophila melanogaster (Fruit fly) (6)
4 of 10
4 of 10
3 of 10
3 of 10
2 of 10
2 of 10
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Model Summary Ribbon
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 0 )
    Human Ortholog
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 3 )
    Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    Functional Complementation Data
    Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
    Interactions
    Summary of Physical Interactions
    Summary of Genetic Interactions
    esyN Network Diagram
    esyN Network Key:
    Suppression
    Enhancement

    Please look at the allele data for full details of the genetic interactions
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    suppressible
    enhanceable
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    External Data
    Subunit Structure (UniProtKB)
    Interacts with a member of the CDK2/CDK protein kinases to form a serine/threonine kinase holoenzyme complex (PubMed:11565033). The cyclin subunit imparts substrate specificity to the complex (PubMed:11565033). Interacts (via C-terminus) with Z600 (via C-terminus) (PubMed:17431409).
    (UniProt, P54733 )
    Linkouts
    BioGRID - A database of protein and genetic interactions.
    DroID - A comprehensive database of gene and protein interactions.
    InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
    MIST (genetic) - An integrated Molecular Interaction Database
    MIST (protein-protein) - An integrated Molecular Interaction Database
    Pathways
    Signaling Pathways (FlyBase)
    Positive Regulators of JAK-STAT Signaling Pathway -
    Positive regulators of JAK-STAT signaling up-regulate the pathway, enhancing transcriptional control by Stat92E.
    Metabolic Pathways
    External Data
    Genomic Location and Detailed Mapping Data
    Chromosome (arm)
    2L
    Recombination map
    2-51
    Cytogenetic map
    Sequence location
    2L:15,727,581..15,748,150 [-]
    FlyBase Computed Cytological Location
    Cytogenetic map
    Evidence for location
    35D4-35D4
    Limits computationally determined from genome sequence between P{EP}esgEP633&P{PZ}esg07082 and P{lacW}CycEk05007&P{lacW}CycEk02602
    Experimentally Determined Cytological Location
    Cytogenetic map
    Notes
    References
    35D1-35D2
    (determined by in situ hybridisation) 35D3--4 (determined by in situ hybridisation)
    35D3-35D4
    (determined by in situ hybridisation)
    35D-35D
    (determined by in situ hybridisation)
    Experimentally Determined Recombination Data
    Location
    Left of (cM)
    Right of (cM)
    Notes
    Stocks and Reagents
    Stocks (44)
    Genomic Clones (18)
     

    Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

    cDNA Clones (246)
     

    Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

    cDNA clones, fully sequenced
    BDGP DGC clones
    Other clones
    Drosophila Genomics Resource Center cDNA clones

    For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

    cDNA Clones, End Sequenced (ESTs)
    Other clones
    RNAi and Array Information
    Linkouts
    DRSC - Results frm RNAi screens
    GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
    Antibody Information
    Laboratory Generated Antibodies
     

    polyclonal

    monoclonal, polyclonal

    Commercially Available Antibodies
     
    Other Information
    Relationship to Other Genes
    Source for database identify of

    Source for identity of: CycE CG3938

    Source for database merge of
    Additional comments
    Other Comments

    S2 cells transfected with dsRNA made from templates generated with primers directed against this gene show a slowing down in G1 phase show a slowing down in G1 phase.

    RNAi generated by PCR using primers directed to this gene causes a cell growth and viability phenotype when assayed in Kc167 and S2R+ cells.

    RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a cell growth and viability phenotype when assayed in Kc167 and S2R+ cells.

    dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.

    RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a phenotype when assayed in Kc167 and S2R+ cells: cells become round and detached, cell size is increased, microtubules are uniform or disorganised, cell shape is irregular, and cell number is decreased indicative of a failure in cell cycle progression through G1 to S and G2 to M stages.

    CycE has a large and complex cis-regulatory region containing tissue- and stage- specific components. Separate regulatory elements for transcription in epidermal cells during cycles 14-16, central nervous system cells and peripheral nervous system cells were found. An additional cis-regulatory element drives transcription in thoracic epidermal cells that undergo a 17th cell cycle.

    Candidate gene for tibia length quantitative trait locus.

    Overexpression of CycE suggests that CycE levels must fluctuate for S phase to occur in successive endocycles. Replication inhibition can be overcome by the kinase inhibitor 6-DMAP, but not by expression of E2f.

    CycE can have both a positive and a negative role in the control of DNA replication.

    Transcript 3' UTR contains three consensus Rbp9 binding sites.

    CycE has a role in cell proliferation control during eye imaginal disc development.

    CycE protein can drive chromosome association of Mcm2 in polytene nuclei.

    Ectopic expression of CycE causes transient ectopic endoreplication, followed by inhibition of the subsequent normal endoreplication program.

    CycE is required and pulses of ectopic expression are sufficient, for triggering endoreplication of S phases. CycE activity, which triggers DNA replication, needs to be down-regulated to allow a subsequent S phases in vivo.

    In vitro studies demonstrate rux binds CycE but does not inhibit CycE-Cdk activity directly.

    E2f transcription factor-induced S phase requires CycE.

    Isolated by interaction with cdc2 and cdc2c using the interaction trap two hybrid system.

    The endocycle is controlled by CycE and lacks a checkpoint ensuring S phase completion.

    Overexpression of dap during eye development interferes with cell cycle progression and interacts genetically with Rbf and CycE.

    During G1 of cycle 17 in the endocycling cells, E2f activation is independent of CycE, whereas CycE expression requires E2f. In the CNS CycE is expressed by a route independent of E2f, and the activation of E2f depends on CycE. The hierarchical relationship of CycE and E2f is reversed by tissue-specific distinctions in the mode of expression of CycE.

    CycE is essential for progression through S phase.

    Correct regulation of CycE is important during development for the coordination of cell proliferation with developmental processes.

    CycE is a target for regulatory mechanisms that coordinate cell proliferation with other developmental events.

    CycE is required in embryos for S phase of mitotic and endoreduplication cycles. Regulatory differences characterise CycE expression in these two cell types. Expression in endoreduplicating cells is restricted by a negative feedback to the transient pulse triggering entry into S phase. During mitotic cycles CycE and cdc2c kinase activity are present throughout the cell cycle. Reinitiation of DNA replication during the G2 phase of the mitotic cell cycle, therefore, is prevented by CycE/cdc2c kinase-independent regulation. Observations in CycA mutants implicate G2 cyclins in this regulation.

    The coordinate program of expression of S phase genes (DNApol-α180, mus209, RnrL and RnrS) can be induced by CycE expression, but is not disrupted in stg mutants and is therefore not a secondary consequence of cell cycle progression.

    Yeast two-hybrid system has been used to characterise Cyclin-dependent kinase interactor proteins (the prey) in hunts with either cdc2 or cdc2c as baits. CycE interacts with cdc2 to activate transcription of a reporter gene, Ecol\lacZ.

    Down regulation of CycE is essential for the arrest of cell proliferation during embryogenesis. CycE is required for progression through S phase. Ectopic expression after the final mitosis prevents the normal G1 arrest and results in progression through an additional cell cycle. cdc2c kinase specifically associates with CycE in vivo.

    The yeast interaction trap selection has been used to isolate cell cycle regulatory proteins that interact with cdc2 and cdc2c. CycE interacts preferentially with cdc2.

    3 additional alleles are discussed but are not named.

    Origin and Etymology
    Discoverer
    Etymology
    Identification
    External Crossreferences and Linkouts ( 103 )
    Sequence Crossreferences
    NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
    GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
    RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
    UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
    UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
    Other crossreferences
    BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
    Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
    EMBL-EBI Single Cell Expression Atlas
    Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
    Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
    Flygut - An atlas of the Drosophila adult midgut
    GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
    iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
    InterPro - A database of protein families, domains and functional sites
    KEGG Genes - Molecular building blocks of life in the genomic space.
    MARRVEL_MODEL
    modMine - A data warehouse for the modENCODE project
    SignaLink - A signaling pathway resource with multi-layered regulatory networks.
    Linkouts
    BioGRID - A database of protein and genetic interactions.
    DroID - A comprehensive database of gene and protein interactions.
    DRSC - Results frm RNAi screens
    FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
    FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
    FlyMine - An integrated database for Drosophila genomics
    Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
    InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
    KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.
    MIST (genetic) - An integrated Molecular Interaction Database
    MIST (protein-protein) - An integrated Molecular Interaction Database
    Synonyms and Secondary IDs (43)
    Reported As
    Symbol Synonym
    Cdi7
    CycE
    (Ahmad et al., 2021, Cabasso et al., 2021, Doherty et al., 2021, Ferguson et al., 2021, Khan et al., 2021, Kim et al., 2021, Yang and Choi, 2021, Cho et al., 2020, Hassan et al., 2020, Hinnant et al., 2020, Hur et al., 2020, Jung et al., 2020, La Marca and Richardson, 2020, Mishra et al., 2020, Neal et al., 2020, Parker et al., 2020, Sharma et al., 2020, Tamamouna et al., 2020, Wang et al., 2020, Williams et al., 2020, Zeng et al., 2020, Zhao et al., 2020, Bivik Stadler et al., 2019, Curt et al., 2019, Drummond-Barbosa, 2019, Fahey-Lozano et al., 2019, Grendler et al., 2019, Harding and White, 2019, Jia et al., 2019, Kim and Choi, 2019, Leiblich et al., 2019, Murcia et al., 2019, Palu et al., 2019, Rodriguez-Fernandez et al., 2019, Simoes da Silva et al., 2019, Simon et al., 2019, Singh et al., 2019, Sun et al., 2019, Wong et al., 2019, Yee et al., 2019, Aughey et al., 2018, Azuma et al., 2018, Drelon et al., 2018, Eusebio et al., 2018, Hao et al., 2018, Kim et al., 2018, Richardson and Portela, 2018, Shen et al., 2018, Wang et al., 2018, Wu et al., 2018, Hevia et al., 2017, Li et al., 2017, Neto et al., 2017, Shu and Deng, 2017, Suisse et al., 2017, Transgenic RNAi Project members, 2017-, Wu et al., 2017, Xiang et al., 2017, Ables et al., 2016, Becker et al., 2016, Bivik et al., 2016, Bradley-Gill et al., 2016, Fallahi et al., 2016, Le et al., 2016, Qi and Calvi, 2016, Barrios et al., 2015, Bivik et al., 2015, Cao et al., 2015, Doggett et al., 2015, Kohlmaier et al., 2015, Li et al., 2015, Parker et al., 2015, Richardson, 2015.3.11, Sun and Buttitta, 2015, Wang and Baker, 2015, Won et al., 2015, Ashwal-Fluss et al., 2014, Chen et al., 2014, Ciglar et al., 2014, Faisal et al., 2014, Gómez-Lamarca et al., 2014, Lai and Doe, 2014, Liang et al., 2014, Neville et al., 2014, Rembold et al., 2014, Singer et al., 2014, Southall et al., 2014, Wong et al., 2014, Zhang et al., 2014, Zhang et al., 2014, Chai et al., 2013, Chang et al., 2013, Chen et al., 2013, Das et al., 2013, Djiane et al., 2013, Dui et al., 2013, Iovino et al., 2013, Mortimer and Moberg, 2013, Neumüller et al., 2013, Noatynska et al., 2013, Schertel et al., 2013, Southall et al., 2013, Unhavaithaya et al., 2013, Yu et al., 2013, Zhao et al., 2013, Zhou and Luo, 2013, Aliee et al., 2012, Azad et al., 2012, Chen and Verheyen, 2012, Farrell et al., 2012, Herranz et al., 2012, Izutsu et al., 2012, Japanese National Institute of Genetics, 2012.5.21, Kelsom and Lu, 2012, Korenjak et al., 2012, Miles et al., 2012, Murray et al., 2012, Poernbacher et al., 2012, Ruggiero et al., 2012, Sallé et al., 2012, Szuplewski et al., 2012, Tokusumi et al., 2012, Xiao et al., 2012, Ambegaokar and Jackson, 2011, Bangi et al., 2011, Chan et al., 2011, Chen et al., 2011, Faradji et al., 2011, Gilbert et al., 2011, Guest et al., 2011, Lindquist et al., 2011, Miles et al., 2011, Neumüller et al., 2011, Read, 2011, Richter et al., 2011, Sun et al., 2011, Willecke et al., 2011, Zhang et al., 2011, Zhang et al., 2011, Zhao et al., 2011, Baig et al., 2010, Baumgartner et al., 2010, Beam and Moberg, 2010, Berger et al., 2010, Biehs et al., 2010, Buttitta et al., 2010, Callan et al., 2010, Forero et al., 2010, Fox et al., 2010, Genevet et al., 2010, Grzeschik et al., 2010, Herranz et al., 2010, Hsieh et al., 2010, Kannan et al., 2010, LaJeunesse, 2010, Malzer et al., 2010, Mandal et al., 2010, Maqbool et al., 2010, Reddy et al., 2010, Richardson, 2010.6.22, Sun et al., 2010, Swaminathan et al., 2010, Usha and Shashidhara, 2010, Wang et al., 2010, Althoff et al., 2009, Buchon et al., 2009, Demontis and Perrimon, 2009, Kurusu et al., 2009, Metzendorf et al., 2009, Narbonne-Reveau and Lilly, 2009, Simon et al., 2009, Smith-Bolton et al., 2009, Southall and Brand, 2009, Szuplewski et al., 2009, Wang et al., 2009, Yu et al., 2009, Aritakula and Ramasamy, 2008, Bello et al., 2008, Berger et al., 2008, Bowman et al., 2008, Duong et al., 2008, Herranz et al., 2008, Hsu et al., 2008, Narbonne-Reveau et al., 2008, O'Farrell and Kylsten, 2008, Rafel and Milán, 2008, Sun et al., 2008, Wang et al., 2008, Zhao et al., 2008, Zhou et al., 2008, Zielke et al., 2008, Zielke et al., 2008, Anuradha et al., 2007, Bello et al., 2007, Berger et al., 2007, Christensen and Cook, 2007.3.22, Escudero and Freeman, 2007, Gawlinski et al., 2007, Gawlinski et al., 2007, Geng and MacDonald, 2007, Griffiths et al., 2007, Großhans et al., 2007, Hartl et al., 2007, Hayden et al., 2007, Hsu et al., 2007, Isogai et al., 2007, Kugler and Nagel, 2007, Lichtneckert et al., 2007, Magalhaes et al., 2007, Matsuno et al., 2007, Monier et al., 2007, Mortimer and Moberg, 2007, Rodrigues and Bach, 2007, Sandmann et al., 2007, Shibutani et al., 2007, Steinhilb et al., 2007, Tanaka-Matakatsu et al., 2007, Tseng et al., 2007, Tyler and Baker, 2007, Wang et al., 2007, Wang et al., 2007, Zhao et al., 2007, Bello et al., 2006, Cho et al., 2006, Choksi et al., 2006, D'Costa et al., 2006, Friedman and Perrimon, 2006, Hamaratoglu et al., 2006, Ho et al., 2006, Jones et al., 2006, Legent et al., 2006, Liebl et al., 2006, Morris et al., 2006, Mukherjee et al., 2006, Thompson and Cohen, 2006, Vrailas and Moses, 2006, Wang et al., 2006, Willecke et al., 2006, Baonza and Freeman, 2005, Crevel et al., 2005, Greene et al., 2005, Franchini et al., 2004, Loop et al., 2004, Stanyon et al., 2004, Heriche et al., 2003, Gim et al., 2001)
    cdi7
    cycE
    (Zhang et al., 2021, Bajpai et al., 2020, Dai et al., 2020, Melamed and Kalderon, 2020, Lee et al., 2019, Ray et al., 2019, Ahmed-de-Prado and Baonza, 2018, Reilein et al., 2018, Li et al., 2017, Jahanshahi et al., 2016, Terzo et al., 2015, Xie et al., 2015, Huang and Kalderon, 2014, Andersen et al., 2013, Jagut et al., 2013, Lim et al., 2013, Losick et al., 2013, Carney et al., 2012, Hudry et al., 2012, Liu et al., 2012, Wang et al., 2012, Zhai et al., 2012, Baxley et al., 2011, Curtis et al., 2011, Genevet and Tapon, 2011, Lin et al., 2011, Estella and Mann, 2010, Forero et al., 2010, Richardson, 2010.6.8, Shaw et al., 2010, Zhai et al., 2010, Genevet et al., 2009, Nicholson et al., 2009, Ninov et al., 2009, Wang and Kalderon, 2009, Baena-Lopez et al., 2008, Deb et al., 2008, Mitchell et al., 2008, Shimizu et al., 2008, Yu et al., 2008, Zhang et al., 2008, Adryan et al., 2007, Buttitta et al., 2007, Hong et al., 2007, Lu et al., 2007, Polesello and Tapon, 2007, White et al., 2007, Berger et al., 2006, Choe et al., 2006, Hyun et al., 2006, Parker, 2006, Tsuda et al., 2006, Chotard et al., 2005, Wakiyama et al., 2005, Wang et al., 2005, Hidalgo and Griffiths, 2004, Hummel and Zipursky, 2004, Heriche et al., 2003, Hong et al., 2003, Murphy, 2003, Park et al., 2003, Robertson et al., 2003, Wu et al., 2003, Kozlova et al., 2000, Li and Vaessin, 2000, Myster and Duronio, 2000, Glaser et al., 1999, Kerber et al., 1998, Morris et al., 1998, Park et al., 1998, Spradling et al., 1997, Spradling et al., 1995, Foe et al., 1993)
    l(2)05206
    l(2)k02514
    l(2)k02602
    l(2)k05007
    Name Synonyms
    Cyclin E
    (Ai et al., 2020, Romine et al., 2019, Shen et al., 2018, Song et al., 2017, Kotov et al., 2016, Dequéant et al., 2015, Ishidate et al., 2014, Liang et al., 2014, Zhang et al., 2014, Zielke et al., 2014, Aleksic et al., 2013, Bandura et al., 2013, Krivy et al., 2013, Unhavaithaya et al., 2013, Zhou and Luo, 2013, Azzam et al., 2012, Joyce et al., 2012, Kelsom and Lu, 2012, Wang et al., 2012, Boyan and Reichert, 2011, Boyan and Williams, 2011, Gilbert et al., 2011, Guest et al., 2011, Klusza and Deng, 2011, Ouyang et al., 2011, Slattery et al., 2011, Zhang et al., 2011, Beam and Moberg, 2010, Berger et al., 2010, Callan et al., 2010, Fox et al., 2010, Hilgers et al., 2010, Kannan et al., 2010, Althoff et al., 2009, Badouel et al., 2009, Demontis and Perrimon, 2009, Ho et al., 2009, Narbonne-Reveau and Lilly, 2009, Ninov et al., 2009, Szuplewski et al., 2009, Wang and Huang, 2009, Bello et al., 2008, Duong et al., 2008, Gupta et al., 2008, Kotadia et al., 2008, Longworth et al., 2008, Narbonne-Reveau et al., 2008, Neumuller et al., 2008, Rafel and Milán, 2008, Sukhanova and Du, 2008, Zielke et al., 2008, Bello et al., 2007, Escudero and Freeman, 2007, Garcia et al., 2007, Gilbert et al., 2007, Grzeschik et al., 2007, Harvey and Bennett, 2007, Herr et al., 2007, Hong et al., 2007, Kankel et al., 2007, Matsuno et al., 2007, Monier et al., 2007, Mortimer and Moberg, 2007, Mortimer and Moberg, 2007, Tyler and Baker, 2007, Beach et al., 2006, Betschinger et al., 2006, Cho et al., 2006, Edgar, 2006, Ho et al., 2006, Khurana et al., 2006, Mukherjee et al., 2006, Reber et al., 2006, Silva, 2006, Silva et al., 2006, Thompson and Cohen, 2006, Vrailas and Moses, 2006, Vrailas et al., 2006, Baonza and Freeman, 2005, Firth and Baker, 2005, LeBrasseur, 2005, Mandal et al., 2005, Furukawa et al., 2003, Gim et al., 2001, Helin, 1998)
    Cyclin-dependent kinase interactor 7
    cyclin E
    (Gaziova et al., 2020, Drelon et al., 2019, Moon et al., 2018, Bradley-Gill et al., 2016, Chung et al., 2016, Parker et al., 2015, Powell et al., 2015, Zhang et al., 2015, Ikmi et al., 2014, Chai et al., 2013, Enderle and McNeill, 2013, Kwon et al., 2013, Losick et al., 2013, Noatynska et al., 2013, Chen and Verheyen, 2012, Laprise, 2011, Andersen et al., 2010, Das Thakur et al., 2010, Gause et al., 2010, LaJeunesse, 2010, Mandal et al., 2010, Mesquita et al., 2010, Epstein et al., 2009, Nicholson et al., 2009, Simon et al., 2009, Ueishi et al., 2009, Wang and Kalderon, 2009, Chia et al., 2008, Deb et al., 2008, Herranz et al., 2008, Lin, 2008, Nicolay and Frolov, 2008, Owusu-Ansah et al., 2008, Shimizu et al., 2008, Sugano et al., 2008, Sun et al., 2008, Adryan et al., 2007, Geng and MacDonald, 2007, Grzeschik et al., 2007, Hamaratoglu et al., 2007, Hartl et al., 2007, Owusu-Ansah et al., 2007, Secombe et al., 2007, Steinhilb et al., 2007, Swanhart et al., 2007, Tseng et al., 2007, White et al., 2007, Bennett and Harvey, 2006, Higa et al., 2006, Kotadia et al., 2006, McBride et al., 2006, Morris et al., 2006, Nolo et al., 2006, Parker, 2006, Willecke et al., 2006, Wodarz and Gonzalez, 2006, Findlay et al., 2005, Frolov et al., 2005, Hulf et al., 2005, Hyun et al., 2005, Kramer and Hawley, 2005, Richardson et al., 2005, Amin et al., 2004, Hidalgo and Griffiths, 2004, Kupsco et al., 2004, Humbert et al., 2003, Lee and Orr-Weaver, 2003, Murphy, 2003, Ryoo and Steller, 2003, Reis and Edgar, 2002, Roy and Ingham, 2002, Deng and Lin, 2001, Harper and Elledge, 1998, Su and O'Farrell, 1998)
    Secondary FlyBase IDs
    • FBgn0001984
    • FBgn0010318
    • FBgn0015389
    • FBgn0022237
    Datasets (1)
    Study focus (1)
    Experimental Role
    Project
    Project Type
    Title
    • RNAi_target
    Genome-wide localization of essential replication factors characterized by ChIP-Seq and ChIP-chip.
    References (869)