FlyBase Gene Report: Dmel\Sas-4

General Information

Symbol

Dmel\Sas-4

Species

D. melanogaster

Name

Spindle assembly abnormal 4

Annotation Symbol

CG10061

Feature Type

protein_coding_gene

FlyBase ID

FBgn0011020

Gene Model Status

Stock Availability

6 publicly available

Gene Snapshot

Spindle assembly abnormal 4 (Sas-4) encodes a centriole protein that is essential for centriole assembly. It is recruited to centrioles through an interaction with the centriole protein encoded by ana2, and it helps recruit microtubules to the centriole. [Date last reviewed: 2019-03-14]

Other Summaries

Alliance
Auto summary
Interactive Fly

Also Known As

DSas-4, Sas4, l(3)s2214

Key Links

Genomic Location

Cytogenetic map

Sequence location

3R:7,151,367..7,154,219 [+]

Recombination map

3-48

RefSeq locus

NT_033777 REGION:7151367..7154219

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse GBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

Gene Ontology (GO) Annotations (18 terms)

Molecular Function (2 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

enables molecular adaptor activity

inferred from physical interaction with FLYBASE:Cp190; FB:FBgn0000283, FLYBASE:cnn; FB:FBgn0013765, FLYBASE:Plp; FB:FBgn0086690, FLYBASE:asl; FB:FBgn0261004

(Gopalakrishnan et al., 2011)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

enables tubulin binding

inferred from biological aspect of ancestor with PANTHER:PTN000035614

(assigned by GO_Central )

(Gaudet et al., 2011)

Biological Process (11 terms)

Terms Based on Experimental Evidence (10 terms)

CV Term

Evidence

References

involved_in asymmetric cell division

inferred from direct assay

(Januschke and Gonzalez, 2010)

inferred from mutant phenotype

(Basto et al., 2006)

involved_in centriole replication

inferred from mutant phenotype

(Dobbelaere et al., 2008, Peel et al., 2007, Basto et al., 2006)

involved_in centrosome cycle

inferred from mutant phenotype

(Gopalakrishnan et al., 2011, Blachon et al., 2009, Rodrigues-Martins et al., 2008, Basto et al., 2006)

involved_in centrosome separation

inferred from mutant phenotype

(Gallaud et al., 2014)

involved_in cilium assembly

inferred from mutant phenotype

(Basto et al., 2006)

involved_in locomotion

inferred from mutant phenotype

(Basto et al., 2006)

involved_in male meiotic nuclear division

inferred from mutant phenotype

(Rodrigues-Martins et al., 2008)

involved_in microtubule polymerization

inferred from mutant phenotype

(Gallaud et al., 2014)

involved_in protein localization to pericentriolar material

inferred from mutant phenotype

(Gopalakrishnan et al., 2011)

involved_in syncytial blastoderm mitotic cell cycle

inferred from mutant phenotype

(Rodrigues-Martins et al., 2008)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

involved_in centriole elongation

inferred from biological aspect of ancestor with PANTHER:PTN000035614

(assigned by GO_Central )

(Gaudet et al., 2011)

involved_in cilium assembly

inferred from biological aspect of ancestor with PANTHER:PTN000035614

(assigned by GO_Central )

(Gaudet et al., 2011)

Cellular Component (5 terms)

Terms Based on Experimental Evidence (3 terms)

CV Term

Evidence

References

colocalizes_with centriole

inferred from direct assay

(Gottardo et al., 2016)

located_in centriole

inferred from direct assay

(Stevens et al., 2010, Blachon et al., 2009, Dobbelaere et al., 2008, Goshima et al., 2007, Peel et al., 2007, Basto et al., 2006)

located_in cytoplasm

inferred from high throughput direct assay

(Sarov et al., 2016)

located_in pericentriolar material

inferred from direct assay

(Gopalakrishnan et al., 2011)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

located_in centriole

inferred from biological aspect of ancestor with PANTHER:PTN000035614

(assigned by GO_Central )

(Gaudet et al., 2011)

located_in centrosome

inferred from biological aspect of ancestor with PANTHER:PTN000035614

(assigned by GO_Central )

(Gaudet et al., 2011)

located_in nucleus

inferred from biological aspect of ancestor with PANTHER:PTN000035614

(assigned by GO_Central )

(Gaudet et al., 2011)

Gene Group (FlyBase)

Protein Family (UniProt)

-

Protein Signatures (InterPro)

Summaries

Summary (Interactive Fly)

centriolar constituent - required for centriolar assembly, centriole replication, flagellum biogenesis, cilium biogenesis and locomotion - regulates asymmetric cell division

Interactive Fly

Gene Model and Products

Number of Transcripts

1

Number of Unique Polypeptides

1

Please see the JBrowse view of Dmel\Sas-4 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Comments on Gene Model

Gene model reviewed during 5.47

Low-frequency RNA-Seq exon junction(s) not annotated.

Sequence Ontology: Class of Gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

FBtr0081569

2802

901

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

FBpp0081088

103.3

901

7.79

Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

Q9VI72

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Sas-4 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Sas-4_1

Linkouts

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

colocalizes_with centriole

inferred from direct assay

(Gottardo et al., 2016)

located_in centriole

inferred from direct assay

(Stevens et al., 2010, Blachon et al., 2009, Dobbelaere et al., 2008, Goshima et al., 2007, Peel et al., 2007, Basto et al., 2006)

located_in cytoplasm

inferred from high throughput direct assay

(Sarov et al., 2016)

located_in pericentriolar material

inferred from direct assay

(Gopalakrishnan et al., 2011)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Sas-4 in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Sas-4

View exonic expression by tissue for Dmel\Sas-4

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0011020

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG10061-RA

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG10061

Flygut - An atlas of the Drosophila adult midgut

FBgn0011020

Images

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 3 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 31 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Sas-4

Transgenic constructs containing regulatory region of Sas-4

Aberrations (Deficiencies and Duplications) ( 3 )

Inferred from experimentation

Gene disrupted in

(Spradling et al., 1999, BDGP Project Members, 1994-1999)

(Gresens and Cook, 2006.12.5)

(Roote, 2004.7.20)

Inferred from location

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

Sas-4^s2214

lethal | recessive

Sas-4^s2214

lethal - all die during P-stage, with Scer\GAL4^Mef2.PR

Sas-4^GD6852

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Sas-4^GD6852

viable, with Scer\GAL4^pnr-MD237

Sas-4^GD6852

viable, with Scer\GAL4^tin.CΔ4

Sas-4^GD6852

Sterility

Allele

Sas-4^s2214

Other Phenotypes

Allele

abnormal cell cycle | somatic clone

Sas-4^s2214

abnormal cell polarity | somatic clone

Sas-4^s2214

abnormal cytokinesis

Sas-4^s2214

abnormal developmental rate

Sas-4^s2214

abnormal mating

Sas-4^s2214

abnormal meiotic cell cycle

Sas-4^s2214

abnormal mitotic cell cycle

Sas-4^s2214

abnormal mitotic cell cycle | third instar larval stage

Sas-4^s2214

increased cell number | third instar larval stage

Sas-4^s2214

increased occurrence of cell division | third instar larval stage

Sas-4^s2214

neoplasia | third instar larval stage

Sas-4^s2214

Sas-4^s2214

some die during immature adult stage | recessive

Sas-4^s2214

Sas-4^s2214

uncoordinated (with Sas-4^s2214)

uncoordinated (with Sas-4^Δ90)

Sas-4^s2214

uncoordinated (with Sas-4^Δ150)

Sas-4^s2214

uncoordinated (with Sas-4^ΔPN2-3)

Sas-4^s2214

uncoordinated | recessive

Sas-4^s2214

visible, with Scer\GAL4^pnr-MD237

Sas-4^GD6852

Phenotype manifest in

Allele

aster, with Sas-4^s2214

Sas-4^ΔT.GFP

aster, with Sas-4^ΔT.GFP

Sas-4^s2214

central protocerebral neuroblast | third instar larval stage

Sas-4^s2214

Sas-4^s2214

centriole | ectopic, with Scer\GAL4^{VP16.mat.αTub67C}

Sas-4^UASp.GFP

centriole | progressive

Sas-4^s2214

Sas-4^s2214

ciliary rootlet

Sas-4^s2214

Sas-4^s2214

embryonic/larval brain

Sas-4^s2214

larval neuroblast | increased number | third instar larval stage

Sas-4^s2214

male germline stem cell

Sas-4^unspecified

male germline stem cell | progressive

Sas-4^s2214

mitotic spindle | third instar larval stage

Sas-4^s2214

Sas-4^s2214

Sas-4^s2214

primary spermatocyte

Sas-4^s2214

sensory neuron | embryonic stage 16

Sas-4^s2214

Sas-4^s2214

spermatid axoneme, with Sas-4^s2214

Sas-4^ΔT.GFP

spermatid axoneme, with Sas-4^ΔT.GFP

Sas-4^s2214

Sas-4^s2214

Sas-4^s2214

trichogen cell | increased number, with Scer\GAL4^pnr-MD237

Sas-4^GD6852

Orthologs

Downloads

Download All DIOPT Orthologs

Download All OrthoDB Orthologs

Human Orthologs (via DIOPT v8.0)

Homo sapiens (Human) (5)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

11 of 15

Yes

Yes

1

4 of 15

No

Yes

3 of 15

No

Yes

3 of 15

No

Yes

Hsap\C21orf59-TCP10L

1 of 15

No

Yes

Model Organism Orthologs (via DIOPT v8.0)

Mus musculus (laboratory mouse) (4)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

10 of 15

Yes

Yes

7 of 15

No

Yes

6 of 15

No

Yes

6 of 15

No

Yes

Rattus norvegicus (Norway rat) (2)

6 of 13

Yes

Yes

3 of 13

No

Yes

Xenopus tropicalis (Western clawed frog) (2)

2 of 12

Yes

Yes

Xtro\LOC100493055

1 of 12

No

Yes

Danio rerio (Zebrafish) (3)

8 of 15

Yes

Yes

Drer\si:ch211-140l13.3

4 of 15

No

Yes

1 of 15

No

Yes

Caenorhabditis elegans (Nematode, roundworm) (1)

1 of 15

Yes

No

Arabidopsis thaliana (thale-cress) (0)

No records found.

Saccharomyces cerevisiae (Brewer's yeast) (0)

No records found.

Schizosaccharomyces pombe (Fission yeast) (0)

No records found.

Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG091902HT )

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila suzukii

Spotted wing Drosophila

Dsuz\DS10_00009340

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Drosophila persimilis

Drosophila willistoni

Drosophila virilis

Drosophila mojavensis

Drosophila grimshawi

Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091501U2 )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Musca domestica

House fly

Lucilia cuprina

Australian sheep blowfly

Lcup\FF38_04604

Mayetiola destructor

Hessian fly

Mdes\Mdes010469

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL008183

Anopheles darlingi

American malaria mosquito

Adar\ADAC009999

Anopheles gambiae

Malaria mosquito

Agam\AGAP001897

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ008369

Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W00ND )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Bombyx mori

Silkmoth

Bmor\BGIBMGA014521

Danaus plexippus

Monarch butterfly

Dple\DPOGS206915-PA

Heliconius melpomene

Postman butterfly

Hmel\HMEL014347

Apis florea

Little honeybee

Aflo\2327959732

Apis mellifera

Western honey bee

Bombus impatiens

Common eastern bumble bee

Bombus terrestris

Buff-tailed bumblebee

Linepithema humile

Argentine ant

Megachile rotundata

Alfalfa leafcutting bee

Mrot\MROTU:002011

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG012450

Pediculus humanus

Human body louse

Phum\PHUM392780

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_07870

Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X00M3 )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strigamia maritima

European centipede

Smar\SMAR010796

Strigamia maritima

European centipede

Smar\SMAR004592

Ixodes scapularis

Black-legged tick

Isca\ISCW015508

Tetranychus urticae

Two-spotted spider mite

Turt\tetur16g01930

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_50490

Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G00Z1 )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strongylocentrotus purpuratus

Purple sea urchin

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Hypp_713

Ciona intestinalis

Vase tunicate

Cint\ENSCING00000010590

Gallus gallus

Domestic chicken

Gallus gallus

Domestic chicken

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v8.0)

Drosophila melanogaster (Fruit fly) (0)

No records found.

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Allele

Disease

Evidence

References

model of microcephaly

CEA with mad2^G6595

(Poulton et al., 2017)

CEA

(Basto et al., 2006)

Potential Models Based on Orthology ( 2 )

Human Ortholog

Disease

Evidence

References

CENPJ; centromere protein J

model of primary autosomal recessive microcephaly 6

IEA

(FlyBase, 2019-)

model of Seckel syndrome 4

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 1 )

Allele

Disease

Interaction

References

exacerbates microcephaly

modeled by Mad1¹

(Poulton et al., 2017)

Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

CENPJ; centromere protein J

11 of 15

TCP10L2; t-complex 10 like 2, pseudogene

4 of 15

TCP10L3; t-complex 10 like 3, pseudogene

3 of 15

TCP10L; t-complex 10 like

3 of 15

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

View in Interactions Browser

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

(Galletta et al., 2016)

Sas-4 - CG13920

experimental knowledge based

(Guruharsha et al., 2011)

anti tag coimmunoprecipitation, anti tag western blot, two hybrid, pull down, western blot, anti bait coimmunoprecipitation

(Galletta et al., 2016, Zheng et al., 2014)

anti tag coimmunoprecipitation, peptide massfingerprinting

(Januschke et al., 2013)

(Galletta et al., 2016)

anti tag coimmunoprecipitation, western blot, pull down, anti bait coimmunoprecipitation, peptide massfingerprinting

(Gopalakrishnan et al., 2012, Gopalakrishnan et al., 2011)

Sas-4 - Grip128

anti bait coimmunoprecipitation, western blot, pull down, cosedimentation through density gradient, peptide massfingerprinting

(Gopalakrishnan et al., 2012, Gopalakrishnan et al., 2011)

Sas-4 - Grip163

anti bait coimmunoprecipitation, cosedimentation through density gradient, western blot

(Gopalakrishnan et al., 2012)

anti bait coimmunoprecipitation, western blot, cosedimentation through density gradient

(Gopalakrishnan et al., 2012)

anti bait coimmunoprecipitation, cosedimentation through density gradient, western blot

(Gopalakrishnan et al., 2012)

anti bait coimmunoprecipitation, western blot, cosedimentation through density gradient

(Gopalakrishnan et al., 2012)

(Galletta et al., 2016)

(Galletta et al., 2016)

anti bait coimmunoprecipitation, western blot, two hybrid, anti tag coimmunoprecipitation, cosedimentation through density gradient, pull down, peptide massfingerprinting

(Galletta et al., 2016, Gopalakrishnan et al., 2012, Gopalakrishnan et al., 2011)

pull down, anti tag western blot, isothermal titration calorimetry, predetermined participant, anti tag coimmunoprecipitation

(McLamarrah et al., 2020)

experimental knowledge based

(Guruharsha et al., 2011)

(Galletta et al., 2016)

anti tag coimmunoprecipitation, anti tag western blot, x-ray crystallography, anti bait coimmunoprecipitation, western blot, two hybrid, pull down, bio-layer interferometry, predetermined participant, autoradiography

(McLamarrah et al., 2020, Moyer and Holland, 2019, Galletta et al., 2016, Dzhindzhev et al., 2014, Zheng et al., 2014, Cottee et al., 2013)

anti bait coimmunoprecipitation, peptide massfingerprinting, western blot

(Gopalakrishnan et al., 2011)

pull down, autoradiography, western blot, two hybrid, anti bait coimmunoprecipitation, anti tag coimmunoprecipitation, cosedimentation through density gradient, peptide massfingerprinting, anti tag western blot

(Galletta et al., 2016, Galletta et al., 2016, Klebba et al., 2015, Zheng et al., 2014, Gopalakrishnan et al., 2012, Gopalakrishnan et al., 2011, Conduit et al., 2010, Dzhindzhev et al., 2010)

(Galletta et al., 2016)

two hybrid, pull down, western blot, anti bait coimmunoprecipitation, peptide massfingerprinting, anti tag western blot, cosedimentation through density gradient, anti tag coimmunoprecipitation

(Galletta et al., 2016, Conduit et al., 2015, Zheng et al., 2014, Gopalakrishnan et al., 2012, Gopalakrishnan et al., 2011, Conduit et al., 2010)

(Galletta et al., 2016)

(Galletta et al., 2016)

Sas-4 - βTub56D

pull down, western blot, anti tag coimmunoprecipitation, anti bait coimmunoprecipitation, cosedimentation through density gradient

(Gopalakrishnan et al., 2012, Gopalakrishnan et al., 2011)

Sas-4 - γTub23C

anti bait coimmunoprecipitation, cosedimentation through density gradient, western blot, pull down, anti tag coimmunoprecipitation

(Conduit et al., 2015, Zheng et al., 2014, Gopalakrishnan et al., 2012, Gopalakrishnan et al., 2011)

Sas-4 - γTub37C

anti bait coimmunoprecipitation, western blot, anti tag coimmunoprecipitation, pull down, cosedimentation through density gradient

(Conduit et al., 2015, Zheng et al., 2014, Gopalakrishnan et al., 2012, Gopalakrishnan et al., 2011)

Summary of Genetic Interactions

esyN Network Diagram

esyN Network Key:

Suppression

Enhancement

View in Interactions Browser

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

suppressible

(Caous et al., 2015)

enhanceable

(Caous et al., 2015)

enhanceable

(Gallaud et al., 2014)

enhanceable

(Poulton et al., 2017, Caous et al., 2015)

suppressible

(Caous et al., 2015)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

enhanceable

(Caous et al., 2015)

enhanceable

(Franz et al., 2013)

enhanceable

(Gallaud et al., 2014)

enhanceable

(Poulton et al., 2017)

suppressible

(Sabino et al., 2011, Basto et al., 2008)

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

66054

DroID - A comprehensive database of gene and protein interactions.

FBgn0011020

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

40859

MIST (genetic) - An integrated Molecular Interaction Database

Sas-4

MIST (protein-protein) - An integrated Molecular Interaction Database

Sas-4

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

External Data

Linkouts

Genomic Location and Detailed Mapping Data

Chromosome (arm)

3R

Recombination map

3-48

Cytogenetic map

Sequence location

3R:7,151,367..7,154,219 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

84C6-84C7

Limits computationally determined from genome sequence between P{lacW}l(3)L2100^L2100 and P{EP}EP3060^EP3060

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

84C4-84C6

(determined by in situ hybridisation)

(Spradling et al., 1999)

84C4-84C6

(BDGP Project Members, 1994-1999)

Experimentally Determined Recombination Data

Location

3-48

(FlyBase, 2016)

3-44.2

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (6)

14803

y¹ w^67c23; P{EPgy2}Sas-4^EY00044

12119

w¹¹¹⁸; P{lacW}Sas-4^s2214/TM3, Sb¹

35049

y¹ sc^* v¹ sev²¹; P{TRiP.HMS01463}attP2

v106051

P{KK100878}VIE-260B

v17975

w¹¹¹⁸; P{GD6852}v17975

v318391

PBac{fTRG10143.sfGFP-FT}VK00033

Genomic Clones (19)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (1)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0011020

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0011020

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

40859

Antibody Information

Laboratory Generated Antibodies

monoclonal

(Ramani et al., 2018, Gopalakrishnan et al., 2011)

polyclonal

(Novak et al., 2016)

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for identity of: Sas-4 l(3)s2214

(Basto et al., 2006)

Source for database merge of

Source for merge of: l(3)s2214 CG10061

(Toub, 2003.10.22)

Additional comments

Other Comments

Sas-4 is involved in centriole duplication.

(Dobbelaere et al., 2008)

RNAi screen using dsRNA made from templates generated with primers directed against this gene results in the spindles that are either anastral (no γ-tubulin staining at the poles) or monastral (only one pole with γ-tubulin staining) when assayed in S2 cells. This phenotype can be observed when the screen is performed with or without Cdc27 dsRNA.

(Goshima et al., 2007)

Origin and Etymology

Discoverer

Etymology

Named "Sas-4" after the C. elegans ortholog.

(FlyBase, 2009-)

Identification

External Crossreferences and Linkouts ( 27 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

40859

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

AAF54053

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Q9VI72

Other crossreferences

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0011020

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Sas-4_1

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG10061

Flygut - An atlas of the Drosophila adult midgut

FBgn0011020

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

40859

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG10061

modMine - A data warehouse for the modENCODE project

FBgn0011020

Linkouts

BioGRID - A database of protein and genetic interactions.

66054

DroID - A comprehensive database of gene and protein interactions.

FBgn0011020

DRSC - Results frm RNAi screens

FBgn0011020

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0011020

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG10061-RA

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0011020

FlyMine - An integrated database for Drosophila genomics

FBgn0011020

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

40859

MIST (genetic) - An integrated Molecular Interaction Database

Sas-4

MIST (protein-protein) - An integrated Molecular Interaction Database

Sas-4

Synonyms and Secondary IDs (26)

Reported As

Symbol Synonym

CENPJ

(Naveed et al., 2018)

CG10061

(Hosono et al., 2015, Ni et al., 2011.7.19, Müller et al., 2010, Keleman et al., 2009.8.5, Dobbelaere et al., 2008, Goshima et al., 2007, Basto et al., 2006, Provost et al., 2006, Avidor-Reiss et al., 2004, Giot et al., 2003)

CPAP

(Carvalho-Santos et al., 2010)

D-Sas4

(Dobbelaere et al., 2008)

D-sas4

(Yuan et al., 2012)

DSAS-4

(Martins et al., 2010, Rodrigues-Martins et al., 2008, Rodrigues-Martins et al., 2007)

DSas4

(Guha et al., 2008)

DmSas4

(Moyer and Holland, 2019)

Dsas-4

(Lucas and Raff, 2007)

Dsas4

(Reschen et al., 2012)

SAS-4

(Ito et al., 2019, Fu et al., 2015, Klebba et al., 2015, Brownlee and Rogers, 2013, Liu, 2010, Anderson et al., 2009)

SAS4

(Jana et al., 2018, Pimenta-Marques et al., 2016, Lerit et al., 2015, Lerit and Rusan, 2013, Carvalho-Santos et al., 2010, Matos et al., 2009)

Sas-4

(Robinson et al., 2020, Singh et al., 2020, Blake-Hedges and Megraw, 2019, Gambarotto et al., 2019, Jo et al., 2019, Ramani et al., 2018, Riparbelli et al., 2018, Tillery et al., 2018, Dewey and Johnston, 2017, Lattao et al., 2017, Gottardo et al., 2016, Gottardo et al., 2016, Khire et al., 2016, Ricolo et al., 2016, Sarov et al., 2016, Saurya et al., 2016, Baumbach et al., 2015, Chen et al., 2015, Conduit et al., 2015, Homem et al., 2015, Khire et al., 2015, Venkei and Yamashita, 2015, Afonso et al., 2014, Chen et al., 2014, Conduit et al., 2014, Gallaud et al., 2014, Riparbelli et al., 2014, Taniguchi et al., 2014, Zheng et al., 2014, Januschke et al., 2013, Fabian and Brill, 2012, Gopalakrishnan et al., 2012, Zhao et al., 2012, Mottier-Pavie et al., 2011, Sabino et al., 2011, Van De Bor et al., 2011, Dzhindzhev et al., 2010, Goodwin and Vale, 2010, Müller et al., 2010, Swaminathan et al., 2010, Peel et al., 2007)

Sas4

(Dobbelaere et al., 2020, Persico et al., 2020, Persico et al., 2019, Dzhindzhev et al., 2017, Galletta et al., 2016, Gottardo et al., 2016, Vieillard et al., 2015, Winkler et al., 2015, Dzhindzhev et al., 2014, Oliveira and Nasmyth, 2013, Guha et al., 2008)

d-SAS-4

(Hsu et al., 2008)

dCPAP

(Cottee et al., 2013)

dsas-4

(Wang et al., 2011, Januschke and Gonzalez, 2010, Castellanos et al., 2008)

l(3)S2214

(Provost et al., 2006)

l(3)s2214

(Gresens and Cook, 2006.12.5)

sas-4

(Zheng et al., 2020, Dewey and Johnston, 2017, Poulton et al., 2017, Caous et al., 2015, Poulton et al., 2014, Jauffred et al., 2013, Gopalakrishnan et al., 2011, Müller et al., 2010, Blachon et al., 2009)

sas4

(Lapart et al., 2019, Loyer and Januschke, 2018, Carvajal-Gonzalez et al., 2016, Galletta et al., 2016, Novak et al., 2014, Enjolras et al., 2012)

Name Synonyms

DSas-4

(Somma et al., 2008)

Drosophila Sas-4

(Basto et al., 2006)

Spindle assembly abnormal 4

Spindle assembly abnormal 4 ortholog

Spindle assembly abnormal 4 orthologue

(Homem et al., 2015)

Secondary FlyBase IDs

FBgn0037480

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

References (160)