Gene model reviewed during 5.47
4.8, 4.4 (northern blot)
1091 (aa); 123 (kD predicted)
The DAD domain regulates activation via by an autoinhibitory interaction with the GBD/FH3 domain. This autoinhibition is released upon competitive binding of an activated GTPase. The release of DAD allows the FH2 domain to then nucleate and elongate nonbranched actin filaments (By similarity).
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\dia using the Feature Mapper tool.
dia transcript is expressed predominantly in tubular organs, including trachea, salivary glands, Malpighian tubules, the hindgut and the proventriculus, starting at embryonic stage 14. Transcript is localized to the apical side, facing the tube lumen.
GBrowse - Visual display of RNA-Seq signalsView Dmel\dia in GBrowse 2
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
dia is required for establishing and maintaining distinct lateral and basal domains during cellularisation. Mutant embryos show an increased number of membrane extensions and endocytic activity in the basal domain, indicating a suppressing role of dia on membrane invaginations.
dia is required for generation of apical F-actin in diverse types of epithelial tubes in the embryo.
dsRNA directed against this gene causes defects in cytokinesis when tested in an RNAi screen in S2 cells.
RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a binucleation phenotype when assayed in Kc167 cells.
dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.
RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a phenotype when assayed in Kc167 and S2R+ cells: binucleate cells with increased or polarized (uneven) accumulation of F-actin.
dsRNA made from templates generated with primers directed against this gene is tested in an RNAi screen for effects on actin-based lamella formation.
Proliferation defect locus.