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General Information
Symbol
Dmel\Klp3A
Species
D. melanogaster
Name
Kinesin-like protein at 3A
Annotation Symbol
CG8590
Feature Type
FlyBase ID
FBgn0011606
Gene Model Status
Stock Availability
Enzyme Name (EC)
Plus-end-directed kinesin ATPase (5.6.1.3)
Gene Summary
Kinesin-like protein at 3A (Klp3A) encodes a microtubule motor protein involved in chromosome segregation and mitotic spindle morphogenesis. [Date last reviewed: 2019-07-11] (FlyBase Gene Snapshot)
Also Known As

mei-352, DmKlp3A, EG:BACR25B3.9 , fs(1)M4

Key Links
Genomic Location
Cytogenetic map
Sequence location
X:2,599,158..2,604,052 [-]
Recombination map
1-1
RefSeq locus
NC_004354 REGION:2599158..2604052
Sequence
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
Function
GO Summary Ribbons
Gene Ontology (GO) Annotations (22 terms)
Molecular Function (4 terms)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
inferred from direct assay
Terms Based on Predictions or Assertions (3 terms)
CV Term
Evidence
References
enables ATP binding
inferred from electronic annotation with InterPro:IPR001752, InterPro:IPR019821
(assigned by InterPro )
inferred from electronic annotation with InterPro:IPR001752
(assigned by InterPro )
Biological Process (11 terms)
Terms Based on Experimental Evidence (10 terms)
CV Term
Evidence
References
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
involved_in mitotic cell cycle
inferred from high throughput mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
involved_in spindle elongation
inferred from mutant phenotype
Terms Based on Predictions or Assertions (3 terms)
CV Term
Evidence
References
traceable author statement
inferred from electronic annotation with InterPro:IPR001752, InterPro:IPR019821, InterPro:IPR027640
(assigned by InterPro )
inferred from biological aspect of ancestor with PANTHER:PTN002516211
(assigned by GO_Central )
Cellular Component (7 terms)
Terms Based on Experimental Evidence (7 terms)
CV Term
Evidence
References
located_in aster
inferred from direct assay
located_in cytoplasm
inferred from direct assay
inferred from direct assay
located_in midbody
inferred from direct assay
located_in nucleus
inferred from direct assay
located_in spindle midzone
inferred from direct assay
located_in spindle
inferred from direct assay
Terms Based on Predictions or Assertions (0 terms)
Gene Group (FlyBase)
Protein Family (UniProt)
-
Catalytic Activity (EC)
Experimental Evidence
-
Predictions / Assertions
ATP + H(2)O + a kinesin associated with a microtubule at position (n) = ADP + phosphate a kinesin associated with a microtubule at position (n+1, toward the plus end) (5.6.1.3)
Summaries
Gene Snapshot
Kinesin-like protein at 3A (Klp3A) encodes a microtubule motor protein involved in chromosome segregation and mitotic spindle morphogenesis. [Date last reviewed: 2019-07-11]
Gene Group (FlyBase)
KINESINS -
Kinesin superfamily proteins (KIFs) are microtubule motor proteins which use the hydrolysis of ATP to drive directional movement along microtubules. KIFs possess a well-conserved 360 residue globular head domain which binds and hydrolyses ATP and interacts with microtubules. Many KIFs homodimerize via coiled-coil interactions in the stalk region. KIFs bind cargo through their variable tail regions and are involved in transporting organelles, protein complexes, mRNAs and the movement of spindles and chromosomes during cell division. (Adapted from FBrf0219884).
Phenotypic Description (Red Book; Lindsley and Zimm 1992)
mei-352 (R.S. Hawley)
mei-352 females exhibit near normal levels of meiotic exchange but the distribution of crossover events is abnormal (i.e., exchanges are more uniformly distributed per unit length than they are in wild-type). This relaxation of the normal constraints on the distribution of euchromatic exchange is clearly demonstrated by the ability of mei-352 females to allow exchange between the normally achiasmate fourth chromosomes (Sandler and Szauter, 1978). mei-352 does not, however, permit exchanges to occur in heterochromatic intervals (Carpenter and Baker, 1982). It remains to be demonstrated that the reduced fertility and the exchange phenotype are the consequence of the same mutational lesion. Although no increase in spontaneous chromosome aberrations was observed upon direct cytological examination of larval neuroblasts (Gatti, 1979), the mei-352 mutation was shown to increase mitotic chromosome instability approximately threefold when assayed genetically (Baker et al., 1978). This suggests that the mei-352+ gene product is required both during meiosis and mitosis.
Gene Model and Products
Number of Transcripts
2
Number of Unique Polypeptides
1

Please see the JBrowse view of Dmel\Klp3A for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model

Gene model reviewed during 5.50

Sequence Ontology: Class of Gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
FBtr0070448
4527
1212
FBtr0340093
4090
1212
Additional Transcript Data and Comments
Reported size (kB)

4.4 (unknown)

Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
FBpp0070432
135.8
1212
7.00
FBpp0309092
135.8
1212
7.00
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

1212 aa isoforms: Klp3A-PA, Klp3A-PB
Additional Polypeptide Data and Comments
Reported size (kDa)

140 (kD observed); 138 (kD predicted)

Comments
External Data
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Klp3A using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
Polypeptide Expression
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data

During the anaphase/telophase of female meiosis, Klp3A protein localizes to the spindle equator, in the midbody. Unlike its diffuse distribution in male meiosis, Klp3A protein is located in the spindle during metaphase, both in female meiotic cells and in syncytial blastoderm nuclei. Klp3A protein is also detected in the sperm aster and in the radial, monastral array of microtubules established between the two meiosis II spindles ("middle pole").

During late anaphase and telophase of male meiosis, Klp3A protein localizes to the equator of the central spindle.

Marker for
 
Subcellular Localization
CV Term
Evidence
References
located_in aster
inferred from direct assay
located_in cytoplasm
inferred from direct assay
inferred from direct assay
located_in midbody
inferred from direct assay
located_in nucleus
inferred from direct assay
located_in spindle midzone
inferred from direct assay
located_in spindle
inferred from direct assay
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Klp3A in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
EMBL-EBI Single Cell Expression Atlas
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 10 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 10 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of Klp3A
Transgenic constructs containing regulatory region of Klp3A
Aberrations (Deficiencies and Duplications) ( 20 )
Alleles Representing Disease-Implicated Variants
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
Sterility
Allele
Other Phenotypes
Allele
Phenotype manifest in
Allele
contractile ring & meiotic cell cycle | male
spindle & meiotic cell cycle | male
Orthologs
Human Orthologs (via DIOPT v8.0)
Homo sapiens (Human) (6)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
13 of 15
Yes
Yes
11 of 15
No
Yes
2 of 15
No
No
1  
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
Model Organism Orthologs (via DIOPT v8.0)
Mus musculus (laboratory mouse) (5)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
13 of 15
Yes
Yes
2 of 15
No
No
2 of 15
No
No
1  
2 of 15
No
No
1 of 15
No
No
Rattus norvegicus (Norway rat) (6)
11 of 13
Yes
Yes
2 of 13
No
Yes
2 of 13
No
Yes
2 of 13
No
No
2 of 13
No
No
2 of 13
No
No
Xenopus tropicalis (Western clawed frog) (6)
6 of 12
Yes
Yes
3 of 12
No
Yes
2 of 12
No
No
2 of 12
No
No
2 of 12
No
No
1 of 12
No
Yes
Danio rerio (Zebrafish) (7)
8 of 15
Yes
Yes
2 of 15
No
Yes
2 of 15
No
No
2 of 15
No
No
2 of 15
No
Yes
Caenorhabditis elegans (Nematode, roundworm) (3)
11 of 15
Yes
Yes
2 of 15
No
No
1 of 15
No
No
Arabidopsis thaliana (thale-cress) (4)
8 of 9
Yes
Yes
8 of 9
Yes
Yes
7 of 9
No
Yes
1 of 9
No
Yes
Saccharomyces cerevisiae (Brewer's yeast) (0)
No records found.
Schizosaccharomyces pombe (Fission yeast) (0)
No records found.
Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG0919019T )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila suzukii
Spotted wing Drosophila
Drosophila simulans
Drosophila sechellia
Drosophila erecta
Drosophila yakuba
Drosophila ananassae
Drosophila pseudoobscura pseudoobscura
Drosophila pseudoobscura pseudoobscura
Drosophila persimilis
Drosophila persimilis
Drosophila persimilis
Drosophila willistoni
Drosophila virilis
Drosophila virilis
Drosophila mojavensis
Drosophila grimshawi
Drosophila grimshawi
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091500OD )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Glossina morsitans
Tsetse fly
Lucilia cuprina
Australian sheep blowfly
Mayetiola destructor
Hessian fly
Aedes aegypti
Yellow fever mosquito
Anopheles darlingi
American malaria mosquito
Anopheles gambiae
Malaria mosquito
Anopheles gambiae
Malaria mosquito
Culex quinquefasciatus
Southern house mosquito
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W01OU )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Danaus plexippus
Monarch butterfly
Heliconius melpomene
Postman butterfly
Heliconius melpomene
Postman butterfly
Heliconius melpomene
Postman butterfly
Apis florea
Little honeybee
Apis mellifera
Western honey bee
Bombus impatiens
Common eastern bumble bee
Bombus terrestris
Buff-tailed bumblebee
Linepithema humile
Argentine ant
Linepithema humile
Argentine ant
Megachile rotundata
Alfalfa leafcutting bee
Nasonia vitripennis
Parasitic wasp
Dendroctonus ponderosae
Mountain pine beetle
Tribolium castaneum
Red flour beetle
Pediculus humanus
Human body louse
Rhodnius prolixus
Kissing bug
Acyrthosiphon pisum
Pea aphid
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X012Z )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Ixodes scapularis
Black-legged tick
Stegodyphus mimosarum
African social velvet spider
Daphnia pulex
Water flea
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G02I4 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Ciona intestinalis
Vase tunicate
Gallus gallus
Domestic chicken
Paralogs
Paralogs (via DIOPT v8.0)
Drosophila melanogaster (Fruit fly) (26)
4 of 10
3 of 10
2 of 10
2 of 10
2 of 10
2 of 10
2 of 10
2 of 10
2 of 10
2 of 10
2 of 10
2 of 10
2 of 10
2 of 10
2 of 10
2 of 10
2 of 10
2 of 10
2 of 10
2 of 10
2 of 10
2 of 10
2 of 10
2 of 10
1 of 10
1 of 10
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Model Summary Ribbon
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 1 )
    Modifiers Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Interaction
    References
    Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Functional Complementation Data
    Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
    Interactions
    Summary of Physical Interactions
    esyN Network Diagram
    Show neighbor-neighbor interactions:
    Select Layout:
    Legend:
    Protein
    RNA
    Selected Interactor(s)
    Interactions Browser

    Please see the Physical Interaction reports below for full details
    protein-protein
    Physical Interaction
    Assay
    References
    Summary of Genetic Interactions
    esyN Network Diagram
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    External Data
    Linkouts
    BioGRID - A database of protein and genetic interactions.
    DroID - A comprehensive database of gene and protein interactions.
    InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
    MIST (protein-protein) - An integrated Molecular Interaction Database
    Pathways
    Signaling Pathways (FlyBase)
    Metabolic Pathways
    External Data
    Linkouts
    Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
    Genomic Location and Detailed Mapping Data
    Chromosome (arm)
    X
    Recombination map
    1-1
    Cytogenetic map
    Sequence location
    X:2,599,158..2,604,052 [-]
    FlyBase Computed Cytological Location
    Cytogenetic map
    Evidence for location
    3A6-3A6
    Limits computationally determined from genome sequence between P{EP}eghEP804 and P{EP}sggEP1576
    Experimentally Determined Cytological Location
    Cytogenetic map
    Notes
    References
    3A5-3A8
    (determined by in situ hybridisation)
    Mapped by classical genetic and deficiency mapping.
    Experimentally Determined Recombination Data
    Location
    Left of (cM)
    Right of (cM)
    Notes
    Stocks and Reagents
    Stocks (10)
    Genomic Clones (13)
     

    Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

    cDNA Clones (34)
     

    Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

    cDNA clones, fully sequenced
    BDGP DGC clones
    Other clones
      Drosophila Genomics Resource Center cDNA clones

      For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

      cDNA Clones, End Sequenced (ESTs)
      RNAi and Array Information
      Linkouts
      DRSC - Results frm RNAi screens
      GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
      Antibody Information
      Laboratory Generated Antibodies
       
      Commercially Available Antibodies
       
      Other Information
      Relationship to Other Genes
      Source for database identify of
      Source for database merge of

      Source for merge of: EG:BACR25B3.9 Klp3A

      Source for merge of: Klp3A mei-352

      Source for merge of: Klp3A BcDNA:LD21815

      Additional comments

      Experiments strongly suggest that "Klp3A" is the gene responsible for the "mei-352" mutant; Klp3A1 and Klp3A63e4 fail to complement the maternal-effect lethal phenotype of Klp3Amei-352, embryos from Klp3Amei-352/Klp3A1 transheterozygous females have a pronuclear fusion defect identical to the phenotype observed for Klp3A homozygotes and the Klp3Amei-352-bearing chromosome has a glutamic acid to lysine missense mutation in the kinesin-like motor domain of Klp3A that is not present on the parental chromosome.

      Source for merge of Klp3A BcDNA:LD21815 was sequence comparison ( date:990717 ).

      Other Comments

      RNAi screen using dsRNA made from templates generated with primers directed against this gene results in chromosome misalignment on the metaphase spindle when assayed in S2 cells. This phenotype can be observed when the screen is performed with or without Cdc27 dsRNA.

      Mutations in Klp3A alter the distribution of meiotic exchanges without greatly affecting their total frequency.

      Klp3A is required to establish and maintain the correct spacing between daughter nuclei during mitosis in embryos.

      dsRNA made from templates generated with primers directed against this gene in S2 cells causes defects in spindle morphology and dynamics. Spindles are shorter than in wild type and spindle microtubules are highly disorganized. Additionally, the mitotic index of prometaphase and metaphase figures is higher than wild type.

      dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.

      Klp3A is necessary for chromosome segregation and can interact specifically with the centromere. Klp3A requires only centromeric sequences for its action in chromosome segregation.

      The major defect caused by depletion of the Klp3A protein is either in specification of the female pronucleus, or in migration of the male and female pronuclei toward each other.

      Mutations have a dramatic effect on the initiation of cytokinesis in testes probably due to the disruption of microtubule interdigitation in spermatocyte central spindles. Anaphase B spindle elongation is not affected. Klp3A function is important in the establishment or stabilisation of the central spindle and this structure is the source of signals that initiate the cleavage furrow.

      Klp3A function is not essential to viability but is required for fertility in both sexes. The establishment of the prominent central spindle and midbody at late anaphase/telophase requires normal Klp3A activity.

      The sequence of the Klp3A protein has been compared with the sequences of a variety of kinesin family proteins.

      The Klp3A+ gene product is required both during meiosis and mitosis.

      Origin and Etymology
      Discoverer
      Etymology
      Identification
      External Crossreferences and Linkouts ( 42 )
      Sequence Crossreferences
      NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
      GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
      GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
      RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
      UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
      Other crossreferences
      BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
      Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
      EMBL-EBI Single Cell Expression Atlas
      Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
      Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
      Flygut - An atlas of the Drosophila adult midgut
      GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
      iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
      KEGG Genes - Molecular building blocks of life in the genomic space.
      MARRVEL_MODEL
      modMine - A data warehouse for the modENCODE project
      Linkouts
      BioGRID - A database of protein and genetic interactions.
      DroID - A comprehensive database of gene and protein interactions.
      DRSC - Results frm RNAi screens
      FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
      FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
      FlyMine - An integrated database for Drosophila genomics
      InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
      MIST (protein-protein) - An integrated Molecular Interaction Database
      Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
      Synonyms and Secondary IDs (23)
      Reported As
      Symbol Synonym
      BcDNA:LD21815
      Name Synonyms
      Kinesin-Like-Protein-at-3A
      female sterile(1)M4
      meiotic 352
      Secondary FlyBase IDs
      • FBgn0000827
      • FBgn0002711
      • FBgn0010237
      • FBgn0040374
      Datasets (0)
      Study focus (0)
      Experimental Role
      Project
      Project Type
      Title
      References (156)