FlyBase Gene Report: Dmel\Mef2

pharyngeal muscle cell | precursor

(Taylor et al., 1995)

embryonic stage -- adult stage

(Nguyen et al., 1994)

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

organism | faint | ubiquitous

(Fisher et al., 2012)

embryonic stage 5

mesoderm anlage

(Taylor et al., 1995)

embryonic stage 5 -- 9

(Nguyen et al., 1994)

embryonic stage 6 -- 7

ventral furrow

(Lilly et al., 1994)

embryonic stage 7 -- 8

anterior endoderm anlage

(Fisher et al., 2012)

head mesoderm

(Fisher et al., 2012)

trunk mesoderm

(Fisher et al., 2012)

extended germ band stage

pharyngeal muscle cell | precursor

(Lilly et al., 1994)

embryonic stage 9 -- 10

head mesoderm

(Fisher et al., 2012)

trunk mesoderm

(Fisher et al., 2012)

embryonic stage 10

(Lilly et al., 1994)

(Lilly et al., 1994)

(Lilly et al., 1994)

dorsal pharyngeal muscle primordium

(Lilly et al., 1994)

embryonic stage 11 -- 12

cardiogenic mesoderm

(Fisher et al., 2012)

(Fisher et al., 2012)

dorsal vessel primordium

Comment: reported as dorsal vessel specific anlage

(Fisher et al., 2012)

longitudinal trunk visceral muscle primordium

(Fisher et al., 2012)

somatic muscle primordium

Comment: reported as muscle system primordium

(Fisher et al., 2012)

trunk visceral muscle primordium

Comment: reported as muscle system primordium

(Fisher et al., 2012)

trunk mesoderm

(Fisher et al., 2012)

embryonic stage 11 -- 17

(Nguyen et al., 1994)

(Nguyen et al., 1994)

(Nguyen et al., 1994)

embryonic somatic muscle cell

(Lilly et al., 1994)

embryonic stage 13 -- 16

circular intestinal visceral muscle fiber

(Fisher et al., 2012)

dorsal pharyngeal muscle cell

(Fisher et al., 2012)

(Fisher et al., 2012)

larval muscle system

(Fisher et al., 2012)

larval visceral muscle cell

(Fisher et al., 2012)

(Fisher et al., 2012)

longitudinal intestinal visceral muscle fiber

(Fisher et al., 2012)

late embryonic stage

larval visceral muscle cell

(Nguyen et al., 1994)

(Nguyen et al., 1994)

larval pericardial cell

(Nguyen et al., 1994)

(Lilly et al., 1994)

(Lilly et al., 1994)

(Lilly et al., 1994)

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic somatic muscle cell | precursor

(Taylor et al., 1995)

fat body primordium

(Taylor et al., 1995)

embryonic somatic muscle cell

(Taylor et al., 1995)

(Taylor et al., 1995)

(Taylor et al., 1995)

embryonic visceral muscle cell | precursor

(Taylor et al., 1995)

(Taylor et al., 1995)

embryonic stage 5

organism | pair rule expression pattern

(Taylor et al., 1995)

Additional Descriptive Data

Mef2 are detected throughout embryogenesis on northern blots with the highest levels in 4-8 and 8-12 hr embryos. Transcripts are first detected by in situ hybridization in the mesoderm primordium in stage 5 embryos. The expression is not uniform but occurs in a pair rule-like pattern of seven stripes. In stages 7 and 8, Mef2 expression occurs throughout the mesoderm. By stages 8 and 9, the pattern becomes very dynamic. Expression is higher in the dorsal region of the mesoderm than ventrally. By stage 9, patches of expression are seen in the dorsal region of the mesoderm with stripes that extend back toward the midline. In stages 10 and 11, expression is also observed in isolated groups of cells that may be heart precursors in one case, and somatic muscle founder cells in another. By stage 11, prominent expression is seen in progenitors of the visceral musculature and in cells that may contribute to the fat body. Later, Mef2 expression is observed in all muscle types. It is expressed in the three major groups of somatic muscles, the dorsal, lateral and ventral. It is expressed in the pharyngeal muscles of the head and in the visceral muscles of the esophagus, foregut, midgut and hindgut. It is also expressed in the heart in the cardioblasts but not in the pericardial cells. The expression pattern of Mef2 was compared to that of twi.

(Taylor et al., 1995)

Mef2 transcripts are first detected in the ventral furrow during gastrulation. During germ band extension, Mef2 transcripts are limited to the mesodermal layer. In early stage 10 embryos, transcripts are in a single layer of mesoderm in cells that will give rise to pharyngeal muscle. Later in stage 10, the mesoderm separates into two cell layers. Mef2 transcripts are expressed in both the inner layer that gives rise to visceral muscle and heart precursor cells and the outer layer which gives rise to the somatic musculature. Expression is also high in pharyngeal muscle precursors. During germ band retraction, transcripts remain at high levels in the somatic musculature but decline in the visceral muscle and heart precursor cells. In late embryos, transcripts are observed in the somatic musculature and in the dorsal vessel but not in other mesoderm derivatives. Little or no Mef2 expression is observed in stage 11-12 sna or twi mutant embryos.

(Lilly et al., 1994)

Mef2 transcripts are first detected in 0-4hr embryos, remain at significant levels throughout embryogenesis, decrease during larval stages, and increase during pupal stages. Transcripts are first detected at late cellular blastoderm stage and are expressed in all mesodermal cells after invagination. After dorsal migration of the mesodermal layer, expression is restricted to visceral muscle and heart primordia. Later expression is observed in heart precursors and then becomes prominant sequentially in visceral and somatic muscles. Mef2 expression is absent in twi mutant embryos and is only observed at low levels in germ band extended sna mutant embryos.

(Nguyen et al., 1994)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

(Molina and Cripps, 2001)

embryonic stage 6

ventral furrow

(Lilly et al., 1995)

embryonic stage 6 -- 10

mesoderm | segmentally repeated

(Lilly et al., 1995)

extended germ band stage

(Halfon et al., 2002)

(Bour et al., 1995)

embryonic stage 10 -- 17

head mesoderm

(Lilly et al., 1995)

mesoderm | segmentally repeated

(Lilly et al., 1995)

embryonic stage 11

(Halfon et al., 2002)

embryonic stage 11 -- 15

cardiogenic mesoderm

(Chen et al., 2016)

(Chen et al., 2016)

embryonic stage 11 -- 17

(Lilly et al., 1995)

(Lilly et al., 1995)

muscle founder cell of circular trunk visceral muscle primordium

Comment: label visible in a deep focal plane

(Mandal et al., 2004)

embryonic stage 12 -- 13

muscle founder cell of longitudinal trunk visceral muscle primordium

Comment: label visible in a superficial focal plane

(Mandal et al., 2004)

(Duan et al., 2001, Bour et al., 1995)

(Bour et al., 1995)

fusion competent cell

(Duan et al., 2001)

(Duan et al., 2001)

embryonic heart cardioblast

(Han and Bodmer, 2003)

pharyngeal muscle cell

(Bour et al., 1995)

embryonic stage 15

(Bour et al., 1995)

embryonic stage 16

dorsal vessel wall cell of embryonic/larval dorsal vessel

Comment: contractile cells of dorsal vessel

(Trujillo et al., 2016, Wang et al., 2005)

(Fossett et al., 2001)

• nucleus

(Lovato et al., 2002)

• nucleus

(Lovato et al., 2002)

embryonic/larval aorta

• nucleus

(Lovato et al., 2002)

• nucleus

(Lovato et al., 2002)

(Bour et al., 1995, Lilly et al., 1995)

embryonic cardioblast

(Lilly et al., 1995)

(Noveen et al., 2000)

embryonic/larval heart | restricted

(Molina and Cripps, 2001)

larval ostial pore

(Molina and Cripps, 2001)

larval cardial cell

(Molina and Cripps, 2001)

pupal stage P6 -- pharate adult stage P14

mesothoracic tergotrochanter muscle cell

Comment: 30-90 hr APF

(Bryantsev et al., 2012)

pharate adult stage P8 -- P14

indirect flight muscle cell

Comment: 48, 90 hr APF

(Bryantsev et al., 2012)

adult somatic muscle cell of thorax

adult pacemaker neuron

(Blanchard et al., 2010)

LNv neuron

(Blanchard et al., 2010)

LNd neuron

(Blanchard et al., 2010)

adult DN period neuron

(Blanchard et al., 2010)

DN1 neuron

(Blanchard et al., 2010)

DN2 neuron

(Blanchard et al., 2010)

DN3 neuron

(Blanchard et al., 2010)

photoreceptor neuron | subset

(Blanchard et al., 2010)

Comment: 1-14 days post-eclosure

(Bryantsev et al., 2012)

adult ostial pore

• nucleus

(Qian et al., 2008)

(Fitzsimons et al., 2013)

Additional Descriptive Data

Significantly higher levels of Mef2 expression are detected in s-LNv neurons towards the end of the night (ZT21) than the end of day (ZT9), but levels remain the same if the animals are kept in dark-dark conditions.

(Blanchard et al., 2010)

Mef2 protein is expressed in all contractile cells of the embryonic dorsal vessel

(Wang et al., 2005)

All myoblasts in the embryonic dorsal vessel express Mef2 protein.

(Perrin et al., 2004)

Mef2 labels 6 cardioblasts per hemisegment in stage 13 embryos.

(Han and Bodmer, 2003)

Mef2 protein is localized to the nuclei of all embryonic somatic and visceral muscle cells and muscle cell precursors.

(Lovato et al., 2002)

Mef2 is present in the nuclei of all muscular dorsal vessel cells of the embryo. A repeating pattern of six pairs of Mef2-positive cardial cells per segment was seen in the larval heart and each ostium is flanked closely by two of these cells.

(Molina and Cripps, 2001)

Mef2 protein expression is detected throughout the mesoderm during germ band extension. After subdivision of the mesoderm, expression is observed in the cephalic mesoderm and in the primordia for the somatic and visceral musculature. Throughout germ band retraction and in older embryos, Mef2 protein expression persists in the visceral and pharyngeal musculature, as well as in the cardial cells and is detected in segmentally repeating groups of forming somatic muscles.

(Bour et al., 1995)

Mef2 expression is first detected in mesodermal cells in the ventral furrow during cellular blastoderm. During germband extension, it is expressed throughout the mesoderm. From stage 11, it is expressed in all of the muscle precursor cells as well as in the cardioblast cells. Expression becomes restricted to muscle cells and marks all of the muscle cells and their descendents in the embryo.

(Lilly et al., 1995)

Marker for

(Lo et al., 2007, Albrecht et al., 2006, Perrin et al., 2004, Fossett et al., 2001)

embryonic cardioblast

(Zmojdzian and Jagla, 2013, Weiss et al., 2001)

embryonic somatic muscle cell

(Trujillo et al., 2016, Rudolf et al., 2012)

(Johnson et al., 2013)

(Weiss et al., 2001)

muscle cell

(Zmojdzian and Jagla, 2013)

Subcellular Localization

CV Term

Evidence

References

located_in nucleus

inferred from direct assay

(Lovato et al., 2002, Bour et al., 1995, Lilly et al., 1995)

Expression Deduced from Reporters

Reporter: P{GAL4-Mef2.R}

Stage

Tissue/Position (including subcellular localization)

Reference

uterine circular muscle cell

somatic muscle cell

(Haghighi et al., 2003)

adult stage | female

(Kairamkonda and Nongthomba, 2014)

common oviduct circular muscle cell

(Kairamkonda and Nongthomba, 2014)

lateral oviduct circular muscle cell

(Kairamkonda and Nongthomba, 2014)

larval muscle system

(Kester and Nambu, 2011)

embryonic stage 7 -- 17

(Ranganayakulu et al., 1996)

embryonic stage 12 -- 17

myoblast

(Ranganayakulu et al., 1996)

embryonic stage 13 -- 17

somatic muscle cell

(Ranganayakulu et al., 1996)

pupal stage P6 -- pharate adult stage P8

(Banovic et al., 2010)

pupal stage P6

vas deferens

Comment: 30 hr APF

(Susic-Jung et al., 2012)

testis

Comment: 40-46 hr APF

(Susic-Jung et al., 2012)

myotube of testis sheath muscle cell

Comment: 30-45 hr APF

(Susic-Jung et al., 2012)

adult somatic muscle cell

LNv neuron

(Blanchard et al., 2010)

Pdf neuron

(Blanchard et al., 2010)

(Viswanathan et al., 2015)

Reporter: P{GMR44H10-lexA}

Stage

Tissue/Position (including subcellular localization)

Reference

muscle cell

(Parisi et al., 2023)

Reporter: P{IIA237-lacZ}

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 12 -- 13

cardiogenic mesoderm

(Gajewski et al., 1997)

embryonic stage 13 -- 17

(Gajewski et al., 1997)

embryonic stage 16

(Kremser et al., 1999)

(Gajewski et al., 1997)

embryonic stage 17

(Gajewski et al., 1997)

Reporter: P{MB-Switch}

Stage

Tissue/Position (including subcellular localization)

Reference

alpha/beta Kenyon cell

(Buchanan and Davis, 2010)

gamma Kenyon cell

(Buchanan and Davis, 2010)

adult mushroom body

(Awata et al., 2019, Goguel et al., 2011)

Reporter: P{Mef2-GAL4.5xCD}

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 14 -- 15

(Beckett et al., 2008)

myotube

(Beckett et al., 2008)

fusion competent cell

(Beckett et al., 2008)

embryonic/larval hypodermal muscle cell

(Beckett et al., 2008)

(Panz et al., 2012)

(Drechsler et al., 2013)

Reporter: P{Mef2-GAL4.247}

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 4

yolk

(Kim et al., 2007)

embryonic/larval midgut

(Kim et al., 2007)

embryonic/larval hindgut

(Kim et al., 2007)

glial cell of larval ventral nerve cord

(Pauls et al., 2010)

surface layer of the larval mushroom body

(Pauls et al., 2010)

outer layer of the larval mushroom body

(Pauls et al., 2010)

inner layer of the larval mushroom body

(Pauls et al., 2010)

adult mushroom body alpha-lobe

(Tan et al., 2010, Zhao et al., 2009, Krashes et al., 2007)

adult mushroom body beta-lobe

(Tan et al., 2010, Zhao et al., 2009, Krashes et al., 2007)

gamma Kenyon cell

(Flybrain Neuron Database et al., 2018.6.28, Tanaka et al., 2008)

calyx of adult mushroom body

(Tanaka et al., 2008)

adult mushroom body dorsal accessory calyx

(Tanaka et al., 2008)

mushroom body beta lobe posterior layer

(Tanaka et al., 2008)

mushroom body beta lobe surface layer

(Tanaka et al., 2008)

mushroom body alpha lobe posterior layer

(Tanaka et al., 2008)

mushroom body alpha lobe surface layer

(Tanaka et al., 2008)

adult mushroom body | restricted

(Zhao et al., 2009)

ellipsoid body

(Poeck et al., 2008)

nodulus

(Poeck et al., 2008)

fan-shaped body | faint | restricted

(Poeck et al., 2008)

ellipsoid body outer central domain | faint | restricted

(Poeck et al., 2008)

adult CNS glial cell of second optic chiasma | restricted

(Poeck et al., 2008)

adult mushroom body

(Poeck et al., 2008, Thum et al., 2006, Baier et al., 2002, McGuire et al., 2001, Zars et al., 2000)

adult mushroom body gamma-lobe

(Riemensperger et al., 2013, Pauls et al., 2010, Tan et al., 2010, Aso et al., 2009, Zhao et al., 2009, Krashes et al., 2007)

adult mushroom body beta'-lobe | restricted

(Krashes et al., 2007)

adult mushroom body beta'-lobe | faint

(Zhao et al., 2009)

adult mushroom body alpha'-lobe | faint

(Zhao et al., 2009)

adult mushroom body beta'-lobe

(Tan et al., 2010)

adult mushroom body alpha'-lobe

(Tan et al., 2010)

adult mushroom body alpha'-lobe | restricted

(Krashes et al., 2007)

mushroom body beta lobe surface layer | intense

(Aso et al., 2009)

mushroom body alpha lobe surface layer | intense

(Aso et al., 2009)

mushroom body beta lobe posterior layer | intense

(Aso et al., 2009)

mushroom body alpha lobe posterior layer | intense

(Aso et al., 2009)

mushroom body beta lobe core layer

(Aso et al., 2009)

mushroom body alpha lobe core layer

(Aso et al., 2009)

lobula plate | faint

(Aso et al., 2009)

adult gnathal ganglion | faint

(Aso et al., 2009)

glial cell of adult brain

(Aso et al., 2009)

adult mushroom body alpha-lobe

(Riemensperger et al., 2013, Pauls et al., 2010)

adult mushroom body beta-lobe

(Riemensperger et al., 2013, Pauls et al., 2010)

alpha/beta surface Kenyon cell

(Flybrain Neuron Database et al., 2018.6.28)

alpha/beta posterior Kenyon cell

(Flybrain Neuron Database et al., 2018.6.28)

adult mushroom body alpha-lobe

(Flybrain Neuron Database et al., 2018.6.28)

adult Kenyon cell

(Cachero et al., 2020)

adult stage | male

(Blum et al., 2009)

adult mushroom body beta-lobe

(Blum et al., 2009)

adult mushroom body gamma-lobe

(Blum et al., 2009)

Reporter: P{Mef2-lacZ.5}

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 10

(Cripps et al., 1999)

(Cripps et al., 1999)

embryonic stage 11

(Cripps et al., 1999)

trunk visceral muscle primordium

(Cripps et al., 1999)

(Cripps et al., 1999)

embryonic somatic muscle cell | ventral

(Cripps et al., 1999)

(Cripps et al., 1999)

(Cripps et al., 1999)

(Cripps et al., 1999)

(Cripps et al., 1999)

(Cripps et al., 1999)

embryonic stage 14

(Cripps et al., 1999)

Reporter: P{Mef2-lacZ.175.wt}

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 9 -- 10

embryonic myoblast | segmentally repeated

(Cripps et al., 1998)

embryonic stage 11 -- 15

Comment: expression levels substantially decreased at stage 15

(Cripps et al., 1998)

Reporter: P{Mef2-lacZ.I.N}

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 9 -- 11

(Nguyen and Xu, 1998)

somatic muscle primordium

(Nguyen and Xu, 1998)

Reporter: P{Mef2-lacZ.II.N}

Stage

Tissue/Position (including subcellular localization)

Reference

somatic muscle primordium

(Nguyen and Xu, 1998)

somatic trunk mesoderm | restricted | segmentally repeated

(Nguyen and Xu, 1998)

embryonic stage 14

embryonic cardioblast

(Nguyen and Xu, 1998)

(Nguyen and Xu, 1998)

embryonic stage 15 -- 17

(Nguyen and Xu, 1998)

Reporter: P{Mef2-lacZ.mE}

Stage

Tissue/Position (including subcellular localization)

Reference

(Cripps et al., 1999)

(Cripps et al., 1999)

Reporter: P{Mef2-lacZ.mtin1}

Stage

Tissue/Position (including subcellular localization)

Reference

(Cripps et al., 1999)

(Cripps et al., 1999)

(Cripps et al., 1999)

(Cripps et al., 1999)

Reporter: P{Mef2-LexA-VP16.247}

Stage

Tissue/Position (including subcellular localization)

Reference

larval Kenyon cell

(Awasaki et al., 2011)

larval Kenyon cell

(Mohamed et al., 2023)

P-stage

(Awasaki et al., 2011)

(Perisse et al., 2013)

Reporter: P{Mef2-QF2.L}

Stage

Tissue/Position (including subcellular localization)

Reference

muscle cell

(Parisi et al., 2023)

View Dmel\Mef2 in JBrowse

adult muscle cell

(Venkatasubramanian et al., 2019)

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Mef2

View exonic expression by tissue for Dmel\Mef2

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0011656

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

36032/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0011656

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0011656

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0011656

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

Flygut - An atlas of the Drosophila adult midgut

FBgn0011656

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\Mef2

Stages(s) 1-3
Stages(s) 7-8
Stages(s) 9-10
Stages(s) 11-12
Stages(s) 13-16

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 67 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 31 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Mef2

Transgenic constructs containing regulatory region of Mef2

Aberrations (Deficiencies and Duplications) ( 17 )

Inferred from experimentation ( 17 )

Gene not disrupted in

Ab(2R)55-20

(Goldstein et al., 2001)

Df(2R)61-25

(Goldstein et al., 2001)

Df(2R)64-10

(Goldstein et al., 2001)

Df(2R)eve

(Goldstein et al., 2001, Schulz et al., 1996, Lilly et al., 1995, Ranganayakulu et al., 1995)

Df(2R)stan2

(Goldstein et al., 2001)

Inferred from location ( 7 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal, with Scer\GAL4^Mef2.PR

lethal, with Scer\GAL4^tim.UAS

Mef2^EnR.UAS

lethal, with Scer\GAL4^tsh-md621

Mef2^JF03115

lethal (with Df(2R)01D09Y-M073)

Mef2^25-34

lethal (with Df(2R)BSC158)

Mef2^EP2002a

lethal (with Df(2R)X1)

lethal (with Mef2^22-21)

lethal (with Mef2^22-24)

lethal (with Mef2^25-34)

lethal (with Mef2^25-42)

lethal (with Mef2^26-6)

lethal (with Mef2^26-7)

lethal (with Mef2^26-49)

lethal (with Mef2^30-5)

lethal (with Mef2^44-5)

lethal (with Mef2^48-7)

lethal (with Mef2^55-5)

Mef2^22-21

lethal (with Mef2^66-25)

lethal (with Mef2^78-11)

lethal | recessive

lethal - all die before end of P-stage, with Scer\GAL4¹¹⁵¹

lethal - all die during embryonic stage, with Scer\GAL4^69B

Mef2^UAS.cBa

lethal - all die during embryonic stage, with Scer\GAL4^how-24B

Mef2^UAS.cBa

lethal - all die during embryonic stage, with Scer\GAL4^hs.2sev

Mef2^UAS.cBa

lethal - all die during embryonic stage, with Scer\GAL4^insc-Mz1407

Mef2^UAS.cBa

lethal - all die during embryonic stage (with Mef2^30-5)

Mef2^44-5

lethal - all die during embryonic stage (with Mef2^44-5)

Mef2^30-5

lethal - all die during embryonic stage | recessive

lethal - all die during larval stage | heat sensitive (with Mef2^30-5)

Mef2^44-5

lethal - all die during larval stage | heat sensitive (with Mef2^44-5)

Mef2^30-5

lethal - all die during P-stage | heat sensitive (with Mef2^30-5)

Mef2^44-5

lethal - all die during P-stage | heat sensitive (with Mef2^44-5)

Mef2^30-5

partially lethal

partially lethal (with Mef2^22-24)

Mef2^55-5

partially lethal (with Mef2^25-34)

partially lethal (with Mef2^25-42)

Mef2^55-5

partially lethal (with Mef2^26-6)

Mef2^55-5

partially lethal (with Mef2^26-7)

Mef2^55-5

partially lethal (with Mef2^26-49)

partially lethal (with Mef2^30-5)

partially lethal (with Mef2^44-5)

partially lethal (with Mef2^48-7)

Mef2^55-5

partially lethal (with Mef2^55-5)

partially lethal (with Mef2^66-25)

partially lethal (with Mef2^78-11)

partially lethal | heat sensitive (with Mef2^30-5)

Mef2^44-5

partially lethal | heat sensitive (with Mef2^44-5)

Mef2^30-5

partially lethal - majority die, with Scer\GAL4^elav.PLu

Mef2^GD5039

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Mef2^GD5039

viable, with Scer\GAL4^Mhc.PU

Mef2^UAS.cUa

viable, with Scer\GAL4^pnr-MD237

Mef2^GD5039

viable, with Scer\GAL4^tin.CΔ4

Mef2^GD5039

viable (with Mef2^25-34)

Mef2^55-5

viable (with Mef2^55-5)

Mef2^25-34

Sterility

Allele

female sterile

Other Phenotypes

Allele

abnormal circadian rhythm, with Scer\GAL4^cry.PZ

Mef2^EnR.UAS

abnormal flight, with Scer\GAL4^elav.PLu

Mef2^GD5039

abnormal locomotor behavior, with Scer\GAL4^Appl.G1a

Mef2^JF03115

abnormal locomotor behavior, with Scer\GAL4^elav.PLu

Mef2^JF03115

abnormal locomotor rhythm, with Scer\GAL4^P0.5.Pdf

abnormal locomotor rhythm, with Scer\GAL4^tim.UAS

abnormal locomotor rhythm | heat sensitive, with Scer\GAL4^tim.UAS, Scer\GAL80^ts.αTub84B

Mef2^EP1751

abnormal neuroanatomy | adult stage, with Scer\GAL4^elav-C155

Mef2^{UAS.cMa.Tag:HA}

abnormal neurophysiology | adult stage, with Scer\GAL4^ninaE.PT

Mef2^UAS.Tag:HA

abnormal size | adult stage, with Scer\GAL4^tin.CΔ4

Mef2^UAS.cBa

abnormal song, with Scer\GAL4^Appl.G1a

Mef2^JF03115

abnormal song, with Scer\GAL4^elav.PLu

Mef2^JF03115

abnormal song, with Scer\GAL4^nrv2.PS

Mef2^JF03115

decreased efficacy of long-term memory | adult stage, with Scer\GAL4^ey-OK107

Mef2^{UAS.cMa.Tag:MYC}

decreased size | adult stage, with Scer\GAL4^org-1.HN39

Mef2^GD5039

flightless, with Scer\GAL4^Mhc.PU

Mef2^UAS.cUa

flightless | heat sensitive (with Mef2^30-5)

Mef2^44-5

flightless | heat sensitive (with Mef2^44-5)

Mef2^30-5

short lived, with Scer\GAL4^da.Switch.PT

Mef2^JF03115

short lived, with Scer\GAL4^EDTP-DJ694

Mef2^RNAi.2.UAS

short lived, with Scer\GAL4^nSyb.PS

Mef2^UAS.Tag:HA

some die during embryonic stage, with Scer\GAL4^69B

Mef2^UAS.cBa

some die during embryonic stage, with Scer\GAL4^how-24B

Mef2^UAS.cBa

some die during embryonic stage, with Scer\GAL4^hs.2sev

Mef2^UAS.cBa

some die during embryonic stage, with Scer\GAL4^insc-Mz1407

Mef2^UAS.cBa

some die during embryonic stage | recessive

some die during pharate adult stage, with Scer\GAL4¹¹⁵¹

visible, with Scer\GAL4^Bx-MS1096

Mef2^{UAS.ORF.GW.Tag:HA}

visible, with Scer\GAL4^ey.PH

Mef2^UAS.cBa

visible, with Scer\GAL4^pnr-MD237

Mef2^GD5039

visible, with Scer\GAL4^sca-537.4

Mef2^EP2002a

Phenotype manifest in

Allele

A1-7 lateral transverse muscle cell, with Scer\GAL4^twi.PB

Mef2^UAS.cBa

adult head, with Scer\GAL4^ey.PH

Mef2^UAS.cBa

adult heart, with Scer\GAL4^tin.CΔ4

Mef2^UAS.cBa

adult heart ventral longitudinal muscle cell, with Scer\GAL4^org-1.HN39

Mef2^GD5039

adult muscle system, with Scer\GAL4¹¹⁵¹

Mef2^RNAi.2.UAS

adult somatic muscle cell, with Scer\GAL4¹¹⁵¹

antenna, with Scer\GAL4^ey.PH

Mef2^UAS.cBa

centripetal follicle cell

coxal tergal remotor muscle cell (with Mef2^30-5)

Mef2^44-5

coxal tergal remotor muscle cell (with Mef2^44-5)

Mef2^30-5

dorsal appendage

dorsal longitudinal indirect flight muscle cell

dorsal longitudinal indirect flight muscle cell (with Mef2^25-34)

Mef2⁶⁵

dorsal longitudinal indirect flight muscle cell (with Mef2^25-42)

Mef2⁶⁵

dorsal longitudinal indirect flight muscle cell (with Mef2^26-6)

Mef2⁶⁵

dorsal longitudinal indirect flight muscle cell (with Mef2⁶⁵)

dorsal longitudinal indirect flight muscle cell (with Mef2¹¹³)

Mef2⁶⁵

dorsal longitudinal indirect flight muscle cell | heat sensitive (with Mef2^30-5)

Mef2^44-5

dorsal longitudinal indirect flight muscle cell | heat sensitive (with Mef2^44-5)

Mef2^30-5

dorsal longitudinal indirect flight muscle cell | pupal stage, with Scer\GAL4¹¹⁵¹

Mef2^GD5039

dorsal longitudinal indirect flight muscle cell | pupal stage, with Scer\GAL4^Act88F.PB

dorsal vessel wall cell, with Scer\GAL4^twi.PB

Mef2^UAS.cBa

egg

embryo | dorsal closure stage, with Scer\GAL4^da.G32

Mef2^III.UAS

embryo | dorsal closure stage, with Scer\GAL4^en-e16E

Mef2^UAS.cBa

embryonic/first instar larval cuticle, with Scer\GAL4^da.G32

embryonic/larval anterior midgut

Mef2¹¹³

embryonic/larval dorsal vessel | heat sensitive (with Mef2^30-5)

Mef2^44-5

embryonic/larval dorsal vessel | heat sensitive (with Mef2^44-5)

Mef2^30-5

embryonic/larval heart, with Scer\GAL4^twi.PB

Mef2^UAS.cBa

embryonic/larval heart | embryonic stage

embryonic/larval midgut

embryonic/larval midgut | embryonic stage

embryonic/larval midgut | embryonic stage, with Scer\GAL4^twi.PB

Mef2^UAS.cBa

embryonic epidermis, with Scer\GAL4^da.G32

Mef2^UAS.cBa

embryonic epidermis, with Scer\GAL4^en-e16E

Mef2^UAS.cBa

embryonic midgut constriction

Mef2^22-21

embryonic midgut constriction, with Scer\GAL4^twi.PB

Mef2^UAS.cBa

embryonic myoblast

embryonic myoblast, with Scer\GAL4^twi.PB

Mef2^UAS.cBa

gastric caecum primordium

Mef2^P544

eye, with Scer\GAL4^ey.PH

Mef2^UAS.cBa

eye photoreceptor cell | adult stage, with Scer\GAL4^ninaE.PT

Mef2^UAS.Tag:HA

gastric caecum

Mef2¹¹³

Mef2¹¹³

indirect flight muscle cell, with Scer\GAL4^Mhc.PU

Mef2^UAS.cUa

indirect flight muscle cell | pupal stage, with Mef2^GD5039, Scer\GAL4^Act88F.PB

indirect flight muscle cell | pupal stage, with Mef2^P544, Scer\GAL4^Act88F.PB

Mef2^GD5039

indirect flight muscle cell | pupal stage, with Mef2^RNAi.2.UAS, Scer\GAL4^Act88F.PB

Mef2^GD5039

indirect flight muscle cell | pupal stage, with Scer\GAL4¹¹⁵¹

Mef2^GD5039

indirect flight muscle cell | pupal stage, with Scer\GAL4^Act88F.PB

Mef2^GD5039

larval cardial cell, with Scer\GAL4^Mef2.PR

Mef2^UAS.Tag:MYC

larval intersegmental nerve

Mef2^22-21

larval muscle system

Mef2¹¹³

larval pericardial cell, with Scer\GAL4^twi.PB

Mef2^UAS.cBa

mesothoracic tergotrochanter muscle cell

larval somatic muscle cell, with Scer\GAL4^how-24B

Mef2^UAS.cBa

larval somatic muscle cell, with Scer\GAL4^Mef2.PR

Mef2^GD5039

larval somatic muscle cell, with Scer\GAL4^twi.PB

Mef2^UAS.cBa

larval somatic muscle cell | heat sensitive (with Mef2^30-5)

Mef2^44-5

larval somatic muscle cell | heat sensitive (with Mef2^44-5)

Mef2^30-5

lateral oblique dorsal muscle cell (with Mef2^30-5)

Mef2^44-5

lateral oblique dorsal muscle cell (with Mef2^44-5)

Mef2^30-5

lateral transverse muscle cell (with Mef2^30-5)

Mef2^44-5

lateral transverse muscle cell (with Mef2^44-5)

Mef2^30-5

lobe system of adult mushroom body, with Scer\GAL4^elav-C155

Mef2^{UAS.cMa.Tag:HA}

mesonotum, with Scer\GAL4^pnr-MD237

Mef2^GD5039

Mef2^P544

mesothoracic tergotrochanter muscle cell, with Scer\GAL4^Act79B.PB

mesothoracic tergotrochanter muscle cell | heat sensitive (with Mef2^30-5)

Mef2^44-5

mesothoracic tergotrochanter muscle cell | heat sensitive (with Mef2^44-5)

Mef2^30-5

muscle attachment site

Mef2^22-21

muscle cell, with Scer\GAL4¹¹⁵¹

Mef2^UAS.cCa

muscle cell of A1-7 dorsal acute muscle 1 | embryonic stage 15

Mef2^22-21

muscle cell of A1-7 lateral transverse muscle 1 (with Mef2^30-5)

Mef2^44-5

muscle cell of A1-7 lateral transverse muscle 1 (with Mef2^44-5)

Mef2^30-5

muscle cell of A1-7 lateral transverse muscle 2 (with Mef2^30-5)

Mef2^44-5

muscle cell of A1-7 lateral transverse muscle 2 (with Mef2^44-5)

Mef2^30-5

muscle cell of A1-7 lateral transverse muscle 3 (with Mef2^30-5)

Mef2^44-5

muscle cell of A1-7 lateral transverse muscle 3 (with Mef2^44-5)

Mef2^30-5

muscle cell of A1-7 lateral transverse muscle 4, with Scer\GAL4^twi.PB

Mef2^UAS.cBa

muscle founder cell | embryonic stage

Mef2^22-21

myoblast

myoblast, with Scer\GAL4¹¹⁵¹

Mef2^UAS.cCa

myofibril, with Scer\GAL4^Mhc.PU

Mef2^UAS.cUa

neuromuscular junction | embryonic stage

oblique muscle cell

pharyngeal muscle cell

Mef2^22-21

presumptive embryonic/larval muscle system

Mef2^22-21

sarcomere, with Scer\GAL4^Mhc.PU

Mef2^UAS.cUa

somatic muscle cell

tergosternal muscle cell (with Mef2^30-5)

Mef2^44-5

tergosternal muscle cell (with Mef2^44-5)

Mef2^30-5

tormogen cell, with Scer\GAL4^sca-537.4

Mef2^EP2002a

tracheal section, with Scer\GAL4^69B

Mef2^UAS.cBa

tracheal section, with Scer\GAL4^hs.2sev

Mef2^UAS.cBa

trichogen cell, with Scer\GAL4^pnr-MD237

Mef2^GD5039

ventral denticle belt, with Scer\GAL4^da.G32

visceral muscle fiber

wing, with Scer\GAL4^Bx-MS1096

Mef2^{UAS.ORF.GW.Tag:HA}

wing, with Scer\GAL4^elav.PLu

Mef2^GD5039

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (6)

Hsap\MEF2A

11 of 14

Yes

Hsap\MEF2C

11 of 14

Yes

Hsap\MEF2D

10 of 14

Yes

Hsap\MEF2B

7 of 14

Yes

Hsap\BORCS8-MEF2B

5 of 14

Yes

Hsap\SRF

2 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (5)

Rnor\Mef2a

11 of 14

Yes

Rnor\Mef2c

11 of 14

Yes

Rnor\Mef2d

10 of 14

Yes

Rnor\Mef2b

6 of 14

Yes

Rnor\Srf

2 of 14

Mus musculus (laboratory mouse) (6)

Mmus\Mef2a

11 of 14

Yes

Mmus\Mef2c

11 of 14

Yes

Mmus\Mef2d

9 of 14

Yes

Mmus\Gm45929

5 of 14

Yes

Mmus\Mef2b

4 of 14

Yes

Mmus\Srf

2 of 14

Xenopus tropicalis (Western clawed frog) (4)

Xtro\mef2a

9 of 13

Yes

Xtro\mef2c

8 of 13

Yes

Xtro\mef2d

7 of 13

Yes

Xtro\arhgap4

1 of 13

Yes

Danio rerio (Zebrafish) (8)

Drer\mef2aa

11 of 14

Yes

Drer\mef2cb

11 of 14

Yes

Drer\mef2ab

9 of 14

Yes

Drer\mef2ca

9 of 14

Yes

Drer\mef2d

9 of 14

Yes

Drer\mef2b

6 of 14

Yes

Drer\srfa

2 of 14

Drer\srfb

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (2)

Cele\mef-2

9 of 14

Yes

Cele\unc-120

2 of 14

Anopheles gambiae (African malaria mosquito) (3)

Agam\AgaP_AGAP007608

12 of 12

Yes

Agam\AgaP_AGAP010512

2 of 12

Yes

Agam\AgaP_AGAP010511

1 of 12

Arabidopsis thaliana (thale-cress) (120)

Atha\AG

7 of 13

Yes

Atha\AGL6

7 of 13

Yes

Atha\AGL8

7 of 13

Yes

Atha\AGL13

7 of 13

Yes

Atha\AGL15

7 of 13

Yes

Atha\AGL18

7 of 13

Yes

Atha\AGL79

7 of 13

Yes

Atha\AP1

7 of 13

Yes

Atha\CAL

7 of 13

Yes

Atha\SEP1

7 of 13

Yes

Atha\SEP2

7 of 13

Yes

Atha\SEP3

7 of 13

Yes

Atha\SEP4

7 of 13

Yes

Atha\SHP1

7 of 13

Yes

Atha\SHP2

7 of 13

Yes

Atha\STK

7 of 13

Yes

Atha\AGL12

6 of 13

Yes

Atha\AGL14

6 of 13

Yes

Atha\AGL16

6 of 13

Yes

Atha\AGL19

6 of 13

Yes

Atha\AGL20

6 of 13

Yes

Atha\AGL21

6 of 13

Yes

Atha\AGL42

6 of 13

Yes

Atha\AGL44

6 of 13

Yes

Atha\AGL72

6 of 13

Yes

Atha\FLC

6 of 13

Yes

Atha\SVP

6 of 13

Yes

Atha\AGL17

5 of 13

Yes

Atha\AGL24

5 of 13

Yes

Atha\AGL31

5 of 13

Yes

Atha\AGL71

5 of 13

Yes

Atha\AP3

5 of 13

Yes

Atha\MAF3

5 of 13

Yes

Atha\PI

5 of 13

Yes

Atha\TT16

5 of 13

Yes

Atha\MAF1

4 of 13

Yes

Atha\AGL30

3 of 13

Yes

Atha\AGL65

3 of 13

Yes

Atha\AGL66

3 of 13

Yes

Atha\AGL67

3 of 13

Yes

Atha\AGL94

3 of 13

Yes

Atha\AGL104

3 of 13

Yes

Atha\AT4G37435

3 of 13

Yes

Atha\GOA

3 of 13

Yes

Atha\MAF4

3 of 13

Yes

Atha\MAF5

3 of 13

Yes

Atha\AGL23

2 of 13

Yes

Atha\AGL28

2 of 13

Yes

Atha\AGL29

2 of 13

Yes

Atha\AGL33

2 of 13

Yes

Atha\AGL34

2 of 13

Yes

Atha\AGL36

2 of 13

Yes

Atha\AGL38

2 of 13

Yes

Atha\AGL46

2 of 13

Yes

Atha\AGL48

2 of 13

Yes

Atha\AGL49

2 of 13

Yes

Atha\AGL52

2 of 13

Yes

Atha\AGL58

2 of 13

Yes

Atha\AGL59

2 of 13

Yes

Atha\AGL61

2 of 13

Yes

Atha\AGL62

2 of 13

Yes

Atha\AGL64

2 of 13

Yes

Atha\AGL78

2 of 13

Yes

Atha\AGL80

2 of 13

Yes

Atha\AGL82

2 of 13

Yes

Atha\AGL86

2 of 13

Yes

Atha\AGL87

2 of 13

Yes

Atha\AGL90

2 of 13

Yes

Atha\AGL92

2 of 13

Yes

Atha\AGL95

2 of 13

Yes

Atha\AGL96

2 of 13

Yes

Atha\AGL97

2 of 13

Yes

Atha\AGL98

2 of 13

Yes

Atha\AGL99

2 of 13

Yes

Atha\AGL102

2 of 13

Yes

Atha\AGL103

2 of 13

Yes

Atha\AT1G17310

2 of 13

Yes

Atha\AT1G48150

2 of 13

Yes

Atha\AT1G72350

2 of 13

Yes

Atha\AT3G05860

2 of 13

Yes

Atha\AT4G36590

2 of 13

Yes

Atha\AT5G26630

2 of 13

Yes

Atha\AT5G27810

2 of 13

Yes

Atha\AT5G49420

2 of 13

Yes

Atha\AT5G55690

2 of 13

Yes

Atha\PHE1

2 of 13

Yes

Atha\AGL26

1 of 13

Yes

Atha\AGL39

1 of 13

Atha\AGL41

1 of 13

Yes

Atha\AGL43

1 of 13

Yes

Atha\AGL50

1 of 13

Yes

Atha\AGL51

1 of 13

Yes

Atha\AGL53

1 of 13

Yes

Atha\AGL54

1 of 13

Yes

Atha\AGL55

1 of 13

Yes

Atha\AGL56

1 of 13

Atha\AGL57

1 of 13

Yes

Atha\AGL75

1 of 13

Yes

Atha\AGL76

1 of 13

Yes

Atha\AGL77

1 of 13

Yes

Atha\AGL81

1 of 13

Yes

Atha\AGL83

1 of 13

Yes

Atha\AGL85

1 of 13

Yes

Atha\AGL89

1 of 13

Yes

Atha\AGL91

1 of 13

Yes

Atha\AGL93

1 of 13

Yes

Atha\AGL101

1 of 13

Yes

Atha\AGL105

1 of 13

Yes

Atha\AT1G31150

1 of 13

Yes

Atha\AT1G33070

1 of 13

Yes

Atha\AT1G59920

1 of 13

Atha\AT1G59930

1 of 13

Atha\AT3G12510

1 of 13

Yes

Atha\AT4G14530

1 of 13

Yes

Atha\AT5G26865

1 of 13

Yes

Atha\AT5G27944

1 of 13

Yes

Atha\AT5G35120

1 of 13

Atha\AT5G38620

1 of 13

Yes

Atha\AT5G40070

1 of 13

Yes

Atha\AT5G46320

1 of 13

Yes

Saccharomyces cerevisiae (Brewer's yeast) (4)

Scer\SMP1

9 of 13

Yes

Scer\RLM1

8 of 13

Yes

Scer\ARG80

2 of 13

Scer\MCM1

2 of 13

Schizosaccharomyces pombe (Fission yeast) (3)

Spom\mbx2

6 of 12

Yes

Spom\mbx1

3 of 12

Yes

Spom\map1

2 of 12

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Mef2. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (1)

2 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 2 )

Allele

Disease

Evidence

References

Mef2^GD5039

model of attention deficit hyperactivity disorder

CEA

(Klein et al., 2020)

Mef2^UAS.cUa

model of carcinoma

CEA with N^ICN.UAS

(Pallavi et al., 2012)

model of cancer

CEA with N^ICN.UAS

(Harsh and Eleftherianos, 2020)

Potential Models Based on Orthology ( 2 )

Human Ortholog

Disease

Evidence

References

MEF2C; myocyte enhancer factor 2C

model of neurodevelopmental disorder with hypotonia, stereotypic hand movements, and impaired language

IEA

(FlyBase, 2019-)

MEF2A; myocyte enhancer factor 2A

model of coronary artery disease

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 4 )

Allele

Disease

Interaction

References

Mef2^22-21

ameliorates alveolar rhabdomyosarcoma

modeled by Hsap\FOXO1::Hsap\PAX7^UAS.cGa

(Galindo et al., 2014)

Mef2^KG01211a

ameliorates Huntington's disease

modeled by Hsap\HTT^{128Q.1-336.UAS}

(Kaltenbach et al., 2007)

Mef2^X1

ameliorates cardiomyopathy

modeled by Pp2B-14D^{act.tin.C.Tag:FLAG}

(Lee et al., 2014)

modeled by Pp2B-14D^act.UAS.EYFP

(Lee et al., 2014)

ameliorates Huntington's disease

modeled by Hsap\HTT^{128Q.1-336.UAS}

(Kaltenbach et al., 2007)

Mef2^GD5039

ameliorates Friedreich ataxia

modeled by fh¹

(Chen et al., 2016)

DOES NOT exacerbate neurodegenerative disease

modeled by Zzzz\CAG^{CAA.94.UAS.Tag:MYC,Tag:FLAG}

(van Eyk et al., 2011)

modeled by Zzzz\CAG^{93.UAS.Tag:MYC,Tag:FLAG}

(van Eyk et al., 2011)

exacerbates neurodegenerative disease

modeled by Zzzz\CTG^{86.UAS.Tag:MYC,Tag:FLAG}

(van Eyk et al., 2011)

ameliorates amyotrophic lateral sclerosis type 8

modeled by Vap33^{P58S.UAS.Tag:FLAG}

(Sanhueza et al., 2015)

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

MEF2A; myocyte enhancer factor 2A

11 of 14

600660

CORONARY ARTERY DISEASE, AUTOSOMAL DOMINANT, 1; ADCAD1

coronary artery disease

MEF2C; myocyte enhancer factor 2C

11 of 14

600662

NEURODEVELOPMENTAL DISORDER WITH HYPOTONIA, STEREOTYPIC HAND MOVEMENTS, AND IMPAIRED LANGUAGE; NEDHSIL

neurodevelopmental disorder with hypotonia, stereotypic hand movements, and impaired language

MEF2D; myocyte enhancer factor 2D

10 of 14

600663

MEF2B; myocyte enhancer factor 2B

7 of 14

600661

BORCS8-MEF2B; BORCS8-MEF2B readthrough

5 of 14

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

electrophoretic mobility shift assay, autoradiography

(Lovato et al., 2009)

Mef2 - Abd-B

bimolecular fluorescence complementation, fluorescence microscopy

(Baëza et al., 2015)

Mef2 - Ada2b

anti tag coimmunoprecipitation, peptide massfingerprinting

(Weake et al., 2011)

Mef2 - Antp

bimolecular fluorescence complementation, fluorescence microscopy

(Baëza et al., 2015)

Mef2 - Eip78C

two hybrid array

(Shokri et al., 2019)

Mef2 - Hsdl2

anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based

(Rhee et al., 2014)

Mef2 - Paics

anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based

(Rhee et al., 2014)

Mef2 - Rel

two hybrid array

(Shokri et al., 2019)

Mef2 - Scr

bimolecular fluorescence complementation, fluorescence microscopy

(Baëza et al., 2015)

Mef2 - Svil

anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based

(Rhee et al., 2014)

Mef2 - Tbp

anti bait coimmunoprecipitation, western blot, anti tag coimmunoprecipitation

(Clark et al., 2013)

Mef2 - TfIIFβ

anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based

(Rhee et al., 2014)

Mef2 - TfIIS

anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based

(Rhee et al., 2014)

Mef2 - Ubx

bimolecular fluorescence complementation, fluorescence microscopy

(Bischof et al., 2018, Baëza et al., 2015)

Mef2 - abd-A

bimolecular fluorescence complementation, fluorescence microscopy, anti tag coimmunoprecipitation, anti tag western blot

(Bischof et al., 2018, Baëza et al., 2015)

Mef2 - nej

two hybrid

(Lin and Baines, 2019)

Mef2 - sd

anti tag coimmunoprecipitation, anti tag western blot, pull down, western blot

(Bernard et al., 2009, Deng et al., 2009)

Mef2 - twi

pull down, autoradiography

(Bernard et al., 2009)

Mef2 - vg

pull down, autoradiography, anti tag coimmunoprecipitation, anti tag western blot

(Deng et al., 2009)

RNA-RNA

Physical Interaction

Assay

References

Mef2 - mir-124

quantitative reverse transcription pcr

(Sun et al., 2012)

Mef2 - mir-92b

quantitative reverse transcription pcr, luminiscence technology, necessary binding region, western blot

(Chen et al., 2012)

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

enhanceable

(Lovato et al., 2005)

twi|Mef2

enhanceable

(Lovato et al., 2005)

suppressible

CycE

(Jiao et al., 2001)

suppressible

Myc

(Jiao et al., 2001)

suppressible

p38b

(Vrailas-Mortimer et al., 2014)

br|Mef2

enhanceable

twi

(Lovato et al., 2005)

enhanceable

twi

(Lovato et al., 2005)

suppressible

zfh1

(Boukhatmi and Bray, 2018)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

enhanceable

Mef2|twi

(Lovato et al., 2005)

suppressible

(Chen et al., 2016)

enhanceable

(Tan et al., 2024)

suppressible

(Main et al., 2021)

Him

suppressible

(Liotta et al., 2007)

mir-92b

suppressible

(Chen et al., 2012)

p38b

suppressible

(Vrailas-Mortimer et al., 2014)

pnr

enhanceable

(Pena-Rangel et al., 2002)

suppressible

(Lee et al., 2014)

enhanceable

(Lovato et al., 2005)

twi|br

enhanceable