Gene model reviewed during 5.51
2186 (aa); 220 (kD observed); 255 (kD predicted)
O-glycosylation by pgant3 is required for proper secretion and localization to the basal cell layer interface during wing development.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Tig using the Feature Mapper tool.
On northern blots, Tig transcripts are first detected at 6-8hrs, peak at 12-14hrs and decline to a low level by the end embryogenesis. A second peak occurs in the late 2nd instar larval to early third instar larval period. By in situ hybridization, transcripts are first detected in hemocytes at stage 12. By stage 14, they are seen in hemocytes throughout the embryo and in fat body.
Isolated from a pullout assay for proteins contained in clots from larval hemolymph and identified by mass spec.
Tig protein is first detected in 8-10hr embryos on western blots. Levels continue to increase throughout embryogenesis. Tig protein is found throughout the larval stages with a peak at 3rd instar. Levels drop markedly at pupation and remain low in pupae and adults. In embryos, Tig protein is detected in hemocytes and fat body by immunolocalization. It accumulates generally in basement membrane and is found at segmental furrows, at sites where muscle apodemes form, and later, at muscle-epidermis attachment sites. Ventral nerve cord commissures are also stained. In larval musculature, Tig protein is most prominent at the muscle-epidermal attachment sites and is also present in ECM surrounding somatic muscles and in basement membrane underlying the epidermis. In adults, it is found at the Z bands of striated jump muscle.
GBrowse - Visual display of RNA-Seq signalsView Dmel\Tig in GBrowse 2
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Source for identity of: Tig CG11527
S2 cells treated with dsRNA generated against this gene show reduced phagocytosis of Candida albicans compared to untreated cells.
dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.