cofilin, ntf, l(2)k05633, no terminal filament, ADF
AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.
Low-frequency RNA-Seq exon junction(s) not annotated.
Gene model reviewed during 5.52
There is only one protein coding transcript and one polypeptide associated with this gene
Phosphorylated in vitro by protein kinase LIMK1 (PubMed:20026655). Phosphorylation is required for inactivation of tsr and for cell proliferation and axon growth (PubMed:15572110). Phosphorylation is negatively regulated by the panthothenate kinase fbl which catalyzes the first step in the conversion of panthothenic acid to coenzyme A (PubMed:22912811).
Dephosphorylated by protein phosphatase ssh which activates tsr.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\tsr using the Feature Mapper tool.
In stage S9,10 egg chambers, total tsr staining is strong in nurse cells and follicle epithelium, while phosphorylated tsr is strong in nurse cell cytoplasm and ring canals but weak in the follicular epithelium. Both are present in border cells but in distinct patterns. Total tsr is present in an asymmetric pattern with more staining at the leading edge while phosphorylated tsr staining is uniform.
GBrowse - Visual display of RNA-Seq signalsView Dmel\tsr in GBrowse 2
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
dsRNA made from templates generated with primers directed against this gene was tested in an RNAi screen to identify genes that regulate Ca2+ release.
dsRNA directed against this gene causes defects in cytokinesis when tested in an RNAi screen in S2 cells.
dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.
RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a phenotype when assayed in Kc167 and S2R+ cells: binucleate cells with increased or polarized (uneven) accumulation of F-actin.
dsRNA made from templates generated with primers directed against this gene is tested in an RNAi screen for effects on actin-based lamella formation.
tsr regulates actin filament formation throughout the cell cortex in the follicular epithelium.
tsr is required for the formation of terminal filaments during larval development and is also required for the migration of border cells during oogenesis.
tsr mutants show disruption of terminal filament (TF) formation in the ovary. TF precursor cells can be seen scattered in a ring in the anterior region of late third larval instar tsr mutant ovaries. tsr is pupal lethal.
Mutants isolated in a screen of the second chromosome identifying genes affecting disc morphology.
tsr mutant spermatocytes exhibit abnormal positioning and delayed migration of asters to cell poles.
Induced expression of tsr proteins in yeast causes aberrant cell shapes reflecting defects in cytokinesis and/or cell shape maintenance.