FlyBase Gene Report: Dmel\ana

General Information

Symbol

Dmel\ana

Species

D. melanogaster

Name

anachronism

Annotation Symbol

CG8084

Feature Type

protein_coding_gene

FlyBase ID

FBgn0011746

Gene Model Status

Current

Stock Availability

28 publicly available

Gene Summary

anachronism (ana) encodes a secreted glycoprotein that is expressed in glia and at lower levels in neuroblasts. It suppresses neuroblast proliferation. [Date last reviewed: 2019-03-07] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
UniProtKB
Interactive Fly

Also Known As

med

Key Links

Genomic Location

Cytogenetic map

45A1-45A1

Sequence location

2R:9,062,295..9,077,554 [+]

Recombination map

2-60

RefSeq locus

NT_033778 REGION:9062295..9077554

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (1 term)

Molecular Function (0 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (0 terms)

Biological Process (1 term)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

involved_in negative regulation of neuroblast proliferation

inferred from mutant phenotype

(Weng and Cohen, 2012)

Terms Based on Predictions or Assertions (0 terms)

Cellular Component (0 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (0 terms)

Gene Group (FlyBase)

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

anachronism (ana) encodes a secreted glycoprotein that is expressed in glia and at lower levels in neuroblasts. It suppresses neuroblast proliferation. [Date last reviewed: 2019-03-07]

Protein Function (UniProtKB)

Negatively regulates proliferation of neuronal precursor cells, thereby controlling the timing of postembryonic neurogenesis.

(UniProt, Q26307)

Summary (Interactive Fly)

secreted glial glycoprotein that inhibits premature neuroblast proliferation - expressed in glia and at lower levels in neuroblasts

Interactive Fly

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\ana for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q26307)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.49

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

ana-RA

FBtr0088559

NM_057690

2417

474

ana-RB

FBtr0300501

NM_001169622

2426

477

ana-RC

FBtr0339284

NM_001299297

2072

390

Additional Transcript Data and Comments

Reported size (kB)

2.4, 1.7 (northern blot)

(Ebens et al., 1993)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

ana-PA

FBpp0087642

54.0

474

7.53

FBpp0289728

54.4

477

7.68

FBpp0308393

44.4

390

8.53

A0A0B4LFV7

NP_001286226

AHN56024

Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments

Reported size (kDa)

474 (aa); 55 (kD)

(Ebens et al., 1993)

Comments

External Data

Crossreferences

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\ana using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

ana_1
ana_2

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

3.52

Transcript Expression

No Assay Recorded

Stage

Tissue/Position (including subcellular localization)

Reference

larval stage

glial cell

(Ebens et al., 1993)

larval brain

(Ebens et al., 1993)

larval central nervous system | restricted

(Ebens et al., 1993)

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 14 -- 17

presumptive embryonic/larval central nervous system | restricted

(Ebens et al., 1993)

larval stage

adult optic lobe

(Ebens et al., 1993)

larval ventral nerve cord

(Ebens et al., 1993)

third instar larval stage

wing pouch | restricted

(Hersh et al., 2007)

mesonotum | restricted

(Hersh et al., 2007)

Additional Descriptive Data

ana is expressed in four spots in the wing pouch and an additional spot in the notum.

(Hersh et al., 2007)

ana transcripts are expressed in scattered cells throughout the larval CNS. Based on the distribution, morphology and nonneural identity (as demonstrated by double labeling with a neuron-specific marker), these cells are identified as glia.

(Ebens et al., 1993)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

No Assay Recorded

Stage

Tissue/Position (including subcellular localization)

Reference

larval stage

glial cell

(Ebens et al., 1993)

larval brain

(Ebens et al., 1993)

larval central nervous system | restricted

(Ebens et al., 1993)

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 14 -- 17

presumptive embryonic/larval central nervous system | restricted

(Ebens et al., 1993)

larval stage

adult optic lobe

(Ebens et al., 1993)

larval ventral nerve cord

(Ebens et al., 1993)

Additional Descriptive Data

ana protein expression occurs in scattered cells throughout the larval CNS. Based on the distribution, morphology and nonneural identity (as demonstrated by double labeling with a neuron-specific marker), these cells are identified as glia.

(Ebens et al., 1993)

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

Reporter: P{ana-spot.GFP}

Stage

Tissue/Position (including subcellular localization)

Reference

third instar larval stage

wing pouch | restricted

(Hersh et al., 2007)

mesonotum | restricted

(Hersh et al., 2007)

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\ana in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\ana

View exonic expression by tissue for Dmel\ana

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

35913/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0011746

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0011746

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0011746

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG8084

Flygut - An atlas of the Drosophila adult midgut

FBgn0011746

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 13 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 7 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of ana

Transgenic constructs containing regulatory region of ana

Aberrations (Deficiencies and Duplications) ( 4 )

Inferred from experimentation ( 4 )

Gene disrupted in

Df(2R)Np1

(Ebens et al., 1993)

Df(2R)Np6

(Ebens et al., 1993)

Gene not disrupted in

Df(2R)w45-31n

(Ebens et al., 1993)

Df(2R)w45-47n

(Ebens et al., 1993)

Inferred from location ( 5 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

viable

Other Phenotypes

Allele

abnormal smell perception | recessive

decreased occurrence of cell division | somatic clone | third instar larval stage, with Scer\GAL4^elav.PU

ana^LA00274

increased occurrence of cell division | larval stage

ana⁹

increased occurrence of cell division | third instar larval stage

ana⁸

short lived | conditional

ana⁹

wild-type

Phenotype manifest in

Allele

adult optic lobe | adult stage

ana¹

adult optic lobe | third instar larval stage

ana⁸

neuroblast

ana¹

neuroblast | third instar larval stage

ana⁸

thoracic neuroblast | third instar larval stage

ana⁸

type I neuroblast | somatic clone | third instar larval stage

ana^LA00274

type I neuroblast | somatic clone | third instar larval stage, with Scer\GAL4^elav.PU

ana^LA00274

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (0)

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (0)

Mus musculus (laboratory mouse) (0)

Xenopus tropicalis (Western clawed frog) (0)

Danio rerio (Zebrafish) (0)

Caenorhabditis elegans (Nematode, roundworm) (0)

Anopheles gambiae (African malaria mosquito) (1)

Agam\AgaP_AGAP005987

5 of 12

Yes

Arabidopsis thaliana (thale-cress) (0)

Saccharomyces cerevisiae (Brewer's yeast) (0)

Schizosaccharomyces pombe (Fission yeast) (0)

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:ana. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (0)

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

RNA-RNA

Physical Interaction

Assay

References

ana - mir-124

quantitative reverse transcription pcr, luminiscence technology, necessary binding region

(Weng and Cohen, 2012)

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

mir-124

suppressible

ana

(Weng and Cohen, 2012)

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

61751

DroID - A comprehensive database of gene and protein interactions.

FBgn0011746

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-60

Cytogenetic map

45A1-45A1

Sequence location

2R:9,062,295..9,077,554 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

45A1-45A1

Limits computationally determined from genome sequence between P{lacW}babo^k16912 and P{lacW}l(2)k16529^k16529

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

45A-45B

(determined by in situ hybridisation)

(Ebens et al., 1993)

Experimentally Determined Recombination Data

Location

2-60

(FlyBase, 2016)

2-58.1

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (28)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

45502

w¹¹¹⁸; P{GMR24F07-GAL4}attP2

49033

w¹¹¹⁸; P{GMR23E11-GAL4}attP2

49074

w¹¹¹⁸; P{GMR24C07-GAL4}attP2

54738

w¹¹¹⁸; P{GMR23E11-lexA}attP40

70132

w¹¹¹⁸; P{R23E11-p65.AD}attP40

69272

w¹¹¹⁸; P{R24C07-GAL4.DBD}attP2

69830

w¹¹¹⁸; P{R23E11-GAL4.DBD}attP2

27515

y¹ v¹; P{TRiP.JF02665}attP2

Korea

16217

P{R23E11-lexA::GADfl}attP2

16213

P{R23E11-lexA::GADfl}attP40

16215

P{R23E11-lexA::GADfl}su(Hw)attP1

VDRC

v330710

P{VSH330710}attP40

More stocks available...

Genomic Clones (28)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (78)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0011746

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0011746

Antibody Information

Laboratory Generated Antibodies

monoclonal

(Ebens et al., 1993)

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

Gene expression is increased in response to the presence of two copies of Scer\GAL4^hs.PB.

(Liu and Lehmann, 2008)

Shows particularly robust cycling of transcription in adult heads, as assessed by expression analysis using high density oligonucleotide arrays with probe generated during three 12-point time course experiments over the course of 6 days.

(Claridge-Chang et al., 2001)

ana encodes a secreted glial glycoprotein that represses neuroblast proliferation in the larval brain.

(Datta, 1995)

trol acts downstream of ana, it is possible that trol may function to inactivate or bypass ana repression to activate cell proliferation.

(Datta, 1995)

The ana locus controls the proliferation of neuroblasts, but is expressed in glia. In mutants, quiescent neuroblasts enter S phase precociously.

(Ebens et al., 1993)

Required for neuroblast proliferation but expressed in glial cells.

Relationship to Other Genes

Source for database merge of

Additional comments

Nomenclature History

Source for database identify of

Nomenclature comments

Etymology

Synonyms and Secondary IDs (7)

Reported As

Name Synonyms

Anachronism

(Awofala et al., 2012)

anachronism

(Ou et al., 2016, Potdar and Sheeba, 2013, Weng and Cohen, 2012, Ou et al., 2011, Hersh et al., 2007, Barmina et al., 2005, Cirelli et al., 2005, Harbison et al., 2005)

medullaless

Secondary FlyBase IDs

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 45 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

35913

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q26307

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

Q26307

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q26307

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

35913/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0011746

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0011746

FlyMine - An integrated database for Drosophila genomics

FBgn0011746

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG8084

MARRVEL_MODEL - MARRVEL (model organism gene)

35913

Linkouts

BioGRID - A database of protein and genetic interactions.

61751

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0011746

DroID - A comprehensive database of gene and protein interactions.

FBgn0011746

DRSC - Results frm RNAi screens

FBgn0011746

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

ana_1
ana_2

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0011746

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0011746

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG8084

Flygut - An atlas of the Drosophila adult midgut

FBgn0011746

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

References (83)

The following external sites may use different assemblies or annotations than FlyBase.

3.52

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