Gene: Dmel\cher
Filamin, sko, shi kong, FLN, Fil
Please see the JBrowse view of Dmel\cher for information on other features
To submit a correction to a gene model please use the Contact FlyBase form
Low-frequency RNA-Seq exon junction(s) not annotated.
Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.
Gene model reviewed during 5.45
Gene model reviewed during 5.48
Gene model reviewed during 6.32
2210, 838 (aa); 240, 90 (kD observed)
Interacts with Ten-m.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\cher using the Feature Mapper tool.
At embryonic stage 8, cher (as well as Ten-m), is expressed in all furrows and in the posterior midgut. It is expressed in a repetitive pattern at stage 11. By stage 13, it is epressed in the epidermis. At stage 15, cher is expressed in bands of epidermal cells at segmental boundaries, where it colocalizes with Ten-m.
Protein is detected throughout the ovariole. A marked increase in protein is observed in the follicle cells in the germarium and in the region with stage 9 oocytes. cher protein is enriched on the ring canals, nurse cell plasma membranes and the fusome. Ovarian somatic cells and regions that display enrichment of cher include the lateral membranes of the interfollicular stalk, and apical, basal and lateral follicle cell membranes. cher protein is also enriched in the ring canals, the fusome and the plasma membrane of the nurse cells. Expression is high in migrating follicle cell populations.
The 240 kD cher protein is germline-specific, as only the 90 kD protein is detected in ovarectomized flies. The 240 kD cher protein is abundant in ring canals, and is found in both the inner and outer rim of the ring canal. Expression is detected starting between germarium regions 1-2a, and continues through egg chamber development. Nurse cell plasma membrane also stains weakly for the 240 kD protein. The follicular epithelium stains starting at region 2a and continues though oogenesis.
The 90 kD protein is not germline specific. It is detected in the muscle sheath surrounding ovarioles.
GBrowse - Visual display of RNA-Seq signals
View Dmel\cher in GBrowse 2Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Source for merge of: cher sko Filamin
Although dispensable in normal epithelium, cher becomes required in the tumor cells for their growth and invasiveness.
One of 42 Drosophila genes identified as being most likely to reveal molecular and cellular mechanisms of nervous system development or plasticity relevant to human Mental Retardation disorders.
dsRNA made from templates generated with primers directed against this gene is tested in an RNAi screen for effects on actin-based lamella formation.
Reduced expression of cher in follicle cells causes defects in the initial encapsulation of germline cysts and in the migration of border cells through the germline cyst. However, follicle cell morphogenesis is unaffected by point mutations that produce truncated cher proteins. These mutations instead cause defects in ring canal formation.
Mutations in cher disrupt actin filament organisation and compromise membrane integrity during oocyte development, resulting in female sterility.
An EMS mutation exhibits an arrest of ovarian development late in stage 10, nurse cell nuclei fail to be retained in the nurse cell complex.
Defects in cher affect ring canal morphology.
