FlyBase Gene Report: Dmel\mia

General Information

Symbol

Dmel\mia

Species

D. melanogaster

Name

meiosis I arrest

Annotation Symbol

CG10390

Feature Type

protein_coding_gene

FlyBase ID

FBgn0014342

Gene Model Status

Current

Stock Availability

8 publicly available

Gene Summary

meiosis I arrest (mia) encodes a paralog of the generally expressed TBP-associated factor encoded by Taf6. mia is expressed only in differentiating male germ cells, starting in the spermatocyte stage. Along with four other testis-specific TBP-associated factors, the product of mia is required for full levels of transcript production from multiple genes expressed in primary spermatocytes in preparation for meiotic cell cycle progression and spermatid differentiation. [Date last reviewed: 2018-09-13] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
Interactive Fly

Also Known As

TFIID, Taf6L, Taf60-2

Key Links

Genomic Location

Cytogenetic map

83B1-83B1

Sequence location

3R:5,548,115..5,551,168 [-]

Recombination map

3-47.5

RefSeq locus

NT_033777 REGION:5548115..5551168

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse GBrowse

Help me choose

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

Gene Ontology (GO) Annotations (17 terms)

Molecular Function (3 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

enables protein heterodimerization activity

inferred from electronic annotation with InterPro:IPR009072

(assigned by InterPro )

(FlyBase Curators et al., 2004-)

contributes_to RNA polymerase II general transcription initiation factor activity

inferred from biological aspect of ancestor with PANTHER:PTN000027265

(assigned by GO_Central )

(Gaudet et al., 2011)

enables transcription coactivator activity

inferred from biological aspect of ancestor with PANTHER:PTN000027265

(assigned by GO_Central )

(Gaudet et al., 2011)

Biological Process (9 terms)

Terms Based on Experimental Evidence (4 terms)

CV Term

Evidence

References

involved_in male meiosis I

inferred from mutant phenotype

(Lin et al., 1996)

involved_in regulation of gene expression

inferred from mutant phenotype

(White-Cooper et al., 1998)

involved_in spermatid differentiation

inferred from mutant phenotype

(White-Cooper et al., 1998)

involved_in spermatogenesis

inferred from mutant phenotype

(Lin et al., 1996)

Terms Based on Predictions or Assertions (5 terms)

CV Term

Evidence

References

involved_in histone acetylation

inferred from biological aspect of ancestor with PANTHER:PTN000027265

(assigned by GO_Central )

(Gaudet et al., 2011)

involved_in meiotic cell cycle

traceable author statement

(Fuller, 1998)

involved_in positive regulation of transcription by RNA polymerase II

inferred from biological aspect of ancestor with PANTHER:PTN000027265

(assigned by GO_Central )

(Gaudet et al., 2011)

involved_in spermatid development

traceable author statement

(Fuller, 1998)

involved_in transcription initiation from RNA polymerase II promoter

inferred from sequence or structural similarity with HGNC:17305

(Aoyagi and Wassarman, 2000)

inferred from sequence or structural similarity with SGD:S000003080

(Aoyagi and Wassarman, 2000)

inferred from biological aspect of ancestor with PANTHER:PTN000027265

(assigned by GO_Central )

(Gaudet et al., 2011)

Cellular Component (5 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

located_in male germ cell nucleus

inferred from direct assay

(Chen et al., 2005)

located_in nucleolus

inferred from direct assay

(Metcalf and Wassarman, 2007, Chen et al., 2005)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

part_of SAGA complex

inferred from electronic annotation with InterPro:IPR037796

(assigned by InterPro )

(FlyBase Curators et al., 2004-)

part_of SLIK (SAGA-like) complex

inferred from electronic annotation with InterPro:IPR037796

(assigned by InterPro )

(FlyBase Curators et al., 2004-)

part_of transcription factor TFIID complex

inferred from sequence or structural similarity with HGNC:17305

(Aoyagi and Wassarman, 2000)

inferred from sequence or structural similarity with SGD:S000003080

(Aoyagi and Wassarman, 2000)

inferred from biological aspect of ancestor with PANTHER:PTN000027265

(assigned by GO_Central )

(Gaudet et al., 2011)

Gene Group (FlyBase)

TESTIS-SPECIFIC TBP-ASSOCIATED FACTORS

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

Gene Group (FlyBase)

TESTIS-SPECIFIC TBP-ASSOCIATED FACTORS -: Testis-specific TBP-associated factors (tTAFs) are paralogs of the generally expressed TAF subunits of transcription factor IID (TFIID). They are predominantly localized to spermatocyte nucleoli and regulate the transcription of genes necessary for spermatocyte entry into meiosis. (Adapted from FBrf0200958).

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\mia for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.48

Gene model reviewed during 5.43

Gene model reviewed during 5.56

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

mia-RA

FBtr0089313

NM_141290

2054

589

mia-RB

FBtr0300009

NM_001144538

1812

589

mia-RC

FBtr0346136

NM_001300252

2348

589

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

mia-PA

FBpp0088355

66.0

589

9.72

NP_649547

AAF52013

mia-PB

FBpp0289286

66.0

589

9.72

NP_001138010

ACL83469

mia-PC

FBpp0311964

66.0

589

9.72

NP_001287181

AHN57180

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

589 aa isoforms: mia-PA, mia-PB, mia-PC

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\mia using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

mia_1
mia_2

Linkouts

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

RT-PCR

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage | male

testis

(Misra et al., 2017)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage | male

testis

(Metcalf and Wassarman, 2007)

spermatocyte

(Metcalf and Wassarman, 2007)

primary spermatocyte

• nucleolus

(Chen et al., 2005)

Additional Descriptive Data

mia colocalizes with Taf1 in spermatocyte nucleoli.

(Metcalf and Wassarman, 2007)

Turns on specifically in male germ cells soon after initiation of spermatocyte differentiation and persists throughout the remainder of the primary spermatocyte stage. Localizes to the nucleolus.

(Chen et al., 2005)

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in male germ cell nucleus

inferred from direct assay

(Chen et al., 2005)

located_in nucleolus

inferred from direct assay

(Metcalf and Wassarman, 2007, Chen et al., 2005)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\mia in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\mia

View exonic expression by tissue for Dmel\mia

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0014342

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG10390-RA

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG10390

Flygut - An atlas of the Drosophila adult midgut

FBgn0014342

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 7 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 5 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of mia

Transgenic constructs containing regulatory region of mia

Aberrations (Deficiencies and Duplications) ( 6 )

Inferred from experimentation ( 6 )

Gene partially disrupted in

Df(3R)LT

(O'Connor and Tanini, 2009.6.24)

Gene disrupted in

Df(3R)Exel6144

(Ryder, 2004.4.26)

Gene not disrupted in

Df(3L)Pc-MK

(Lin et al., 1996, Lin, 1995.4.19)

Df(3L)ri-79c

(Lin et al., 1996, Lin, 1995.4.19)

Df(3R)2-2

(Lin et al., 1996, Lin, 1995.4.19)

Df(3R)Tpl10

(Lin et al., 1996, Lin, 1995.4.19)

Inferred from location ( 0 )

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

viable

mia^EY07883

viable, with Scer\GAL4^Mef2.PR

mia^GD4141

viable, with Scer\GAL4^pnr-MD237

mia^GD4141

viable (with mia¹)

mia^z-3348

viable (with mia^z-3348)

mia¹

Sterility

Allele

fertile

mia^EY07883

fertile (with mia¹)

mia^z-3348

fertile (with mia^z-3348)

mia¹

male sterile | recessive

mia¹

Phenotype manifest in

Allele

spermatocyte

mia¹

testis

mia¹

Orthologs

Downloads

Download All DIOPT Orthologs

Download All OrthoDB Orthologs

Human Orthologs (via DIOPT v8.0)

Homo sapiens (Human) (2)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Hsap\TAF6

6 of 15

Yes

Hsap\TAF6L

2 of 15

Yes

Model Organism Orthologs (via DIOPT v8.0)

Mus musculus (laboratory mouse) (2)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Mmus\Taf6

5 of 15

Yes

Mmus\Taf6l

2 of 15

Yes

Rattus norvegicus (Norway rat) (2)

Rnor\Taf6

6 of 13

Yes

Rnor\Taf6l

2 of 13

Yes

Xenopus tropicalis (Western clawed frog) (2)

Xtro\taf6

5 of 12

Yes

Xtro\taf6l

2 of 12

Yes

Danio rerio (Zebrafish) (2)

Drer\taf6

6 of 15

Yes

Drer\taf6l

2 of 15

Yes

Caenorhabditis elegans (Nematode, roundworm) (3)

Cele\taf-6.1

9 of 15

Yes

Cele\taf-6.2

3 of 15

Cele\eri-3

1 of 15

Yes

Arabidopsis thaliana (thale-cress) (2)

Atha\TAF6B

3 of 9

Yes

Atha\TAFII59

3 of 9

Yes

Saccharomyces cerevisiae (Brewer's yeast) (1)

Scer\TAF6

7 of 15

Yes

Schizosaccharomyces pombe (Fission yeast) (1)

Spom\taf6

4 of 12

Yes

Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG091906SW )

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila suzukii

Spotted wing Drosophila

Drosophila simulans

Drosophila sechellia

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila willistoni

Dwil\GK13782

Drosophila virilis

Dvir\GJ24553

Drosophila mojavensis

Dmoj\GI24488

Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG0915049T )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Musca domestica

House fly

Mdoa\17014399

Glossina morsitans

Tsetse fly

Gmor\GMOY005929

Lucilia cuprina

Australian sheep blowfly

Lcup\FF38_10042

Mayetiola destructor

Hessian fly

Mdes\Mdes007299

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL006988

Anopheles darlingi

American malaria mosquito

Adar\ADAC010381

Anopheles gambiae

Malaria mosquito

Agam\AGAP004254

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ002544

Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W03UX )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Bombyx mori

Silkmoth

Bmor\BGIBMGA007579

Danaus plexippus

Monarch butterfly

Dple\DPOGS204303-PA

Heliconius melpomene

Postman butterfly

Hmel\HMEL015163

Apis florea

Little honeybee

Aflo\2957033156

Apis mellifera

Western honey bee

Amel\Taf6

Bombus impatiens

Common eastern bumble bee

Bimp\BIMP21831

Bombus terrestris

Buff-tailed bumblebee

Bter\15211625

Linepithema humile

Argentine ant

Lhum\LH19437

Megachile rotundata

Alfalfa leafcutting bee

Mrot\MROTU:0000e3

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG021418

Dendroctonus ponderosae

Mountain pine beetle

Dpod\YQE_06063

Tribolium castaneum

Red flour beetle

Tcas\TC013033

Pediculus humanus

Human body louse

Phum\PHUM376110

Rhodnius prolixus

Kissing bug

Rpro\RPRC006499

Cimex lectularius

Bed bug

Clec\CLEC001878

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI002370

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_14198

Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X03S2 )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strigamia maritima

European centipede

Smar\SMAR009442

Ixodes scapularis

Black-legged tick

Isca\ISCW010253

Stegodyphus mimosarum

African social velvet spider

Smim\KFM60438.1

Stegodyphus mimosarum

African social velvet spider

Smim\KFM60439.1

Tetranychus urticae

Two-spotted spider mite

Turt\tetur02g04120

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_244529

Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G05S9 )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Taf6

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Taf6ll

Ciona intestinalis

Vase tunicate

Cint\ENSCING00000004695

Ciona intestinalis

Vase tunicate

Cint\ENSCING00000018562

Gallus gallus

Domestic chicken

Ggal\ENSGALG00000027878

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v8.0)

Drosophila melanogaster (Fruit fly) (1)

Gene Symbol

Score

Source

Alignment

6 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

TAF6; TATA-box binding protein associated factor 6

6 of 15

602955

ALAZAMI-YUAN SYNDROME; ALYUS

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

Summary of Genetic Interactions

esyN Network Diagram

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0014342

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

40667

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

External Data

Linkouts

KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.

Basal transcription factors

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-47.5

Cytogenetic map

83B1-83B1

Sequence location

3R:5,548,115..5,551,168 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

83B1-83B1

Limits computationally determined from genome sequence between P{PZ}Snr1⁰¹³¹⁹&P{PZ}Itp-r83A⁰⁵⁶¹⁶ and P{lacW}Atu^s1938

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Placed in 78C9--81F or 83A--C2 based on complementation by Df(3L)ri-79c, Df(3L)Pc-MK, Df(3R)2-2, and Df(3R)Tpl10.

(Lin, 1995.4.19)

Experimentally Determined Recombination Data

Location

3-47.5

(FlyBase, 2016)

3-44.1

(Comeron et al., 2012)

3-47.0 +/- 0.2

(Lin et al., 1996, Lin, 1995.4.19)

Left of (cM)

(Lin et al., 1996, Lin, 1995.4.19)

Right of (cM)

(Lin et al., 1996, Lin, 1995.4.19)

Notes

Mapping based on 14 recombinants between kni and Ki.

(Lin et al., 1996)

Stocks and Reagents

Stocks (8)

Bloomington

59528

y¹ w^*; Mi{MIC}mia^MI14528

16865

y¹ w^67c23; P{EPgy2}mia^EY07883

27595

y¹ w^*; Df(3R)LT, mia^LT Pcmt^LT

79914

y¹ sc^* v¹ sev²¹; P{TOE.GS02170}attP40

57790

y¹ sc^* v¹ sev²¹; P{TRiP.HMC04984}attP40/CyO

16244

y¹ w¹¹¹⁸; PBac{5HPw⁺}mia^B560/TM3, Sb¹ Ser¹

VDRC

v100313

P{KK107112}VIE-260B

v37563

w¹¹¹⁸; P{GD4141}v37563/CyO

Genomic Clones (18)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (20)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0014342

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0014342

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

40667

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for identity of: Taf60-2 CG10390

(Aoyagi and Wassarman, 2000)

Source for database merge of

Source for merge of: mia Taf6L

(Hiller et al., 2004)

Additional comments

Other Comments

can, mia and sa are required for accumulation of twe^T:Ecol\lacZ transgene in late primary spermatocytes.

(White-Cooper et al., 1998)

Wild type function of aly, can, mia and sa is required for cell-cycle progression through the G₂/M transition of meiosis I in males and for onset of spermatid differentiation. The phenotype of these meiotic arrest mutants is strikingly similar to the histopathological features of meiosis I maturation arrest infertility in human males, suggesting that the control point may be conserved from flies to man.

(Lin et al., 1996)

mia is required for both the progression of the male meiotic cell cycle and the onset of postmeiotic differentiation.

(Lin et al., 1995)

Mutations in mia cause a complete arrest of spermatogenesis at the G2/M transition to meiosis I, similar to aly, can, and sa.

(Lin, 1995.4.19)

Origin and Etymology

Discoverer

Etymology

Identification

External Crossreferences and Linkouts ( 43 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

40667

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones