Pros26.4, S4, p56, dRpt2
Gene model reviewed during 5.47
Gene model reviewed during 5.56
Interacts with PSMD5.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Rpt2 using the Feature Mapper tool.
Maternal Rpt2 transcripts are distributed uniformly in a stage 4 embryo. Transcripts begin to accumulate in presumptive neuronal cells at stage 11. From stage 11-14, expression is mainly observed in the brain and ventral nerve cord and is found in neuroblasts. Expression is also observed in the posterior spiracles. Expression remains high in proliferating neuroblasts in the ventral nerve cord in third instar larvae and is also observed in the ring gland. In eye disc, expression is mainly seen anterior to the morphogenetic furrow, whereas in the antennal disc, transcript distribution is more uniform. In the wing disc, expression is observed all over in a modulated pattern; it is weaker in presumptive wing vein areas and in the zone of non-proliferation. A similar modulated pattern is observed in the leg disc.
In early stages, Rpt2 protein is detected uniformly in the cytoplasm of all cell types, somatic follicle cells as well as nurse cells and the oocyte. In stage 9, conspicuous accumulation is observed in the geminal vesicle. In embryos, protein expression is uniform and can be detected in the cytoplasm and nuclie starting at stage 5 through to dorsal closure. By stage 14, Rpt2 protein is enriched in the neuromeres of the ventral nerve cord and in the midgut. Nuclear accumulation is mostly apparent in the amnioserosa.
GBrowse - Visual display of RNA-Seq signalsView Dmel\Rpt2 in GBrowse 2
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
The nomenclature of genes encoding subunits of the 26S proteasome of D. melanogaster have been standardized according to FBrf0215459. These symbols/names largely follow those used already in FlyBase, and largely mirror fly community usage. HOWEVER, note that at least one other nomenclature system exists that is followed by the HUGO Gene Nomenclature Committee (HGNC), for example, with the unfortunate result that several D. melanogaster genes have shared synonyms.
RNAi screen using dsRNA made from templates generated with primers directed against this gene results in the formation of short, monastral spindles and severe proliferation defects when assayed in S2 cells. This phenotype can be observed when the screen is performed with or without Cdc27 dsRNA.
RNAi generated by PCR using primers directed to this gene causes a cell growth and viability phenotype when assayed in Kc167 and S2R+ cells.
RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a cell growth and viability phenotype when assayed in Kc167 and S2R+ cells.
p50, p48 and p42b subunits of the regulator complex are selectively protected against proteolysis in a reconstituted 26S protease.