Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\scs using the Feature Mapper tool.
GBrowse - Visual display of RNA-Seq signalsView Dmel\scs in GBrowse 2
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Xenopus oocyte assay demonstrates the scs and scs' insulator elements do not require chromosomal context to block enhancer-activated transcription. A single insulator element partially blocks enhancer-activated transcription indicating that each element operates independently rather than as a pair. Deletion analysis identifies a 220bp fragment that has full enhancer blocking activity. A time course of transcription shows the scs element blocks enhancer-activated transcription prior to complete chromatin assembly. The scs and scs' elements do not block site-specific recombination by Scer\FLP1. These data are most consistent with a model for insulator action in which direct interaction between the insulator and either the enhancer or promoter confers directionality to enhancer-activated transcription.
Results suggest that scs may act as flexible regulatory element that modulates enhancer-promoter interactions within complex promoters and complex genetic loci. The insulator does not propagate changes in chromatin structure and may not be restricted to the functional isolation of neighbouring genetic loci.
The full length or 0.9kb fragment of scs is a chromatin insulator that prevents an enhancer located on one side of a boundary from acting on promoters of neighbouring genes located in the adjacent domain.
The 87A7 Hsp70B region is bordered on the proximal and distal sides by two special chromatin structures, scs and scs'. An enhancer blocking assay based on the w gene demonstrates that the two nuclease hypersensitive regions that define part of the special chromatin structure are essential for the blocking activity. The DNA sequence spanning the nuclease resistant core located between the two hypersensitive regions is dispensable.
The Hsp70Aa and Hsp70Ab loci are flanked by special chromatin structures, scs and scs'. Each structure is defined by a pair of nuclease hypersensitivity sites bordering a nuclease resistant core of 250-350bp in length. Both scs and scs' have properties that suggest they may correspond to the boundaries of the 87A7 chromomere.
scs and scs' are capable of establishing a domain of independent gene activity: w gene flanked by scs and scs' inserted into the genome by P element mediated transformation is isolated against both positive and negative position effects at most insertion sites. scs and scs' also have enhancer blocking activities: when inserted between a Yp1 enhancer and a heat shock promoter-Ecol\lacZ fusion little or no Ecol\lacZ expression is detected even though the promoter is fully heat shock inducible.