DRE-binding factor, dDREF, DNA replication-related element-binding factor, DNA replication-related element binding factor
5' terminus determined by primer extension (FBrf0086456)
Gene model reviewed during 5.43
Gene model reviewed during 5.52
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Dref using the Feature Mapper tool.
Dref expression is high in the dividing and growing cells of the third instar larval eye disc, which are located anterior to the morphogenetic furrow.
Ovarian expression of Dref is strong in nurse cells and follicle cells at 5 days post eclosion, but by 60 days post eclosion very little remains.
Dref is detected in the nuclei in most cells of the adult midgut including intestinal stem cells and enteroblasts.
Up until nuclear cycle 7, weak staining is observed in the nuclei and surrounding cytoplasm of embryos. After cycle 8, strong and uniform nuclear staining is observed.
GBrowse - Visual display of RNA-Seq signalsView Dmel\Dref in GBrowse 2
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
RNAi screen using dsRNA made from templates generated with primers directed against this gene results in chromosome misalignment on the metaphase spindle when assayed in S2 cells. This phenotype can be observed when the screen is performed with or without Cdc27 dsRNA.
Dref is required for normal DNA replication in both the mitotic cell cycle and endo cycle.
A Dref cDNA has been cloned and sequenced. Deletion analysis indicates that part of the N-terminal basic amino acid region is responsible for the specific binding of Dref to the DNA replication-related element (DRE).
Analysis of DNApol-α73-Ecol\CAT deletion constructs shows that full promoter activity is located within the region from -285 to +129bp of DNApol-α73. Three DREs (DNA-replication related elements) are located within this region, and DNA footprinting analysis shows that Dref can bind to all three sites. Mutation of any one of the DRE sites causes extensive reductions in promoter activity and Dref binding to the promoter fragment.
Expression pattern and subcellular distribution of the protein during development is studied using antibodies.
DNApol-α180 and mus209 are negatively regulated by zen protein. This repression is mediated by the DRE (DNA replication-related element) sites in the DNApol-α180 and mus209 promoters. The amount of Dref is reduced in transfected Kc cells expressing zen, suggesting that zen represses expression of DNA replication-related genes by reducing the amount of Dref.
Specific binding factor that binds to the 8bp palindrome required for high expression of DNApol-α and mus209. The 86kD gene product has been purified.