FB2020_06, released Dec 22, 2020

Gene: Dmel\Rab14

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General Information
Symbol
Dmel\Rab14
Species
D. melanogaster
Name
Rab14
Annotation Symbol
CG4212
Feature Type
protein_coding_gene
FlyBase ID
FBgn0015791
Gene Model Status
Current
Stock Availability
17 publicly available
Gene Snapshot
Rab14 (Rab14) encodes a protein involved in phagocytosis, phagolysosome assembly and phagosome acidification. [Date last reviewed: 2019-08-01]
Other Summaries
Also Known As

BG:DS01068.7 , DmRab14, ESTS:57H4T

Key Links
Genomic Location
Cytogenetic map
35A4-35A4
Sequence location
2L:14,355,145..14,358,764 [+]
Recombination map

2-50

RefSeq locus
NT_033779 REGION:14355145..14358764
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
GO Summary Ribbons
Gene Ontology (GO) Annotations (21 terms)
Molecular Function (2 terms)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
enables GTP binding
(assigned by InterPro )
(FlyBase Curators et al., 2004-)
enables GTPase activity
inferred from biological aspect of ancestor with PANTHER:PTN001292747
(assigned by GO_Central )
(Gaudet et al., 2011)
inferred from sequence or structural similarity with FLYBASE:Rab11; FB:FBgn0015790
(FlyBase Curators, 2008--)
Biological Process (10 terms)
Terms Based on Experimental Evidence (4 terms)
CV Term
Evidence
References
involved_in defense response to bacterium
inferred from mutant phenotype
(Garg and Wu, 2014)
involved_in phagolysosome assembly
inferred from mutant phenotype
(Garg and Wu, 2014)
involved_in phagosome acidification
inferred from mutant phenotype
(Garg and Wu, 2014)
involved_in viral entry into host cell
inferred from high throughput mutant phenotype
(Hodgson et al., 2019)
Terms Based on Predictions or Assertions (6 terms)
CV Term
Evidence
References
involved_in endocytic recycling
inferred from electronic annotation with InterPro:IPR030702
(assigned by InterPro )
(FlyBase Curators et al., 2004-)
involved_in Golgi to endosome transport
inferred from biological aspect of ancestor with PANTHER:PTN000633928
(assigned by GO_Central )
(Gaudet et al., 2011)
involved_in intracellular protein transport
inferred from biological aspect of ancestor with PANTHER:PTN001292747
(assigned by GO_Central )
(Gaudet et al., 2011)
involved_in phagolysosome assembly involved in apoptotic cell clearance
inferred from biological aspect of ancestor with PANTHER:PTN000633928
(assigned by GO_Central )
(Gaudet et al., 2011)
involved_in Rab protein signal transduction
inferred from sequence or structural similarity with FLYBASE:Rab11; FB:FBgn0015790
(FlyBase Curators, 2008--)
involved_in vesicle-mediated transport
inferred from sequence or structural similarity with FLYBASE:Rab11; FB:FBgn0015790
(FlyBase Curators, 2008--)
Cellular Component (9 terms)
Terms Based on Experimental Evidence (4 terms)
CV Term
Evidence
References
located_in early endosome
inferred from direct assay
(Garg and Wu, 2014)
located_in late endosome
inferred from direct assay
(Garg and Wu, 2014)
inferred from high throughput direct assay
(Chan et al., 2011)
located_in neuronal cell body
inferred from direct assay
(Jin et al., 2012)
inferred from high throughput direct assay
(Chan et al., 2011)
located_in synapse
inferred from direct assay
(Jin et al., 2012)
Terms Based on Predictions or Assertions (6 terms)
CV Term
Evidence
References
located_in early endosome
inferred from biological aspect of ancestor with PANTHER:PTN000633928
(assigned by GO_Central )
(Gaudet et al., 2011)
located_in endomembrane system
inferred from biological aspect of ancestor with PANTHER:PTN001292747
(assigned by GO_Central )
(Gaudet et al., 2011)
located_in phagocytic vesicle
inferred from biological aspect of ancestor with PANTHER:PTN000633928
(assigned by GO_Central )
(Gaudet et al., 2011)
located_in recycling endosome
inferred from electronic annotation with InterPro:IPR030702
(assigned by InterPro )
(FlyBase Curators et al., 2004-)
located_in trans-Golgi network
inferred from electronic annotation with InterPro:IPR030702
(assigned by InterPro )
(FlyBase Curators et al., 2004-)
located_in vesicle
inferred from sequence or structural similarity with FLYBASE:Rab11; FB:FBgn0015790
(FlyBase Curators, 2008--)
Gene Group (FlyBase)
Protein Family (UniProt)
-
Protein Signatures (InterPro)
Summaries
Gene Group (FlyBase)
RAB GTPASES -
The Rab family are members of the Ras superfamily of small GTPases. Rabs regulate vesicle trafficking including cargo selection, vesicle budding, transport, docking and targeting. They localize to different intracellular compartments directed by specific isoprenylation of C-terminal motifs. (Adapted from PMID:15731001).
Gene Model and Products
Number of Transcripts
3
Number of Unique Polypeptides
2

Please see the JBrowse view of Dmel\Rab14 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model

Gene model reviewed during 5.40

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.52

Sequence Ontology: Class of Gene
nuclear_gene
protein_coding_gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
Rab14-RA
FBtr0080626
NM_057823
1147
215
Rab14-RB
FBtr0080627
NM_176042
1468
239
Rab14-RD
FBtr0343678
NM_176043
878
215
Additional Transcript Data and Comments
Reported size (kB)

1.116 (longest cDNA)

(Satoh et al., 1997)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
Rab14-PA
FBpp0080199
24.3
215
6.13
NP_477171
AAF53390
Rab14-PB
FBpp0080200
27.1
239
7.02
NP_788056
AAO41193
Rab14-PD
FBpp0310268
24.3
215
6.13
NP_788057
AAO41194
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

215 aa isoforms: Rab14-PA, Rab14-PD
Additional Polypeptide Data and Comments
Reported size (kDa)

215 (aa)

(Satoh et al., 1997)
Comments
External Data
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Rab14 using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
expression microarray
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage
eye
(Xu et al., 2004)
Additional Descriptive Data

Eye-enriched transcripts determined by ratio of expression level in wild-type heads. versus expression level in so heads.

(Xu et al., 2004)
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
day 5 of adulthood & male -- day 7 of adulthood & male
spermatozoon
(Wasbrough et al., 2010)
adult stage
adult head
(Aradska et al., 2015)
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
located_in early endosome
inferred from direct assay
(Garg and Wu, 2014)
located_in late endosome
inferred from direct assay
(Garg and Wu, 2014)
inferred from high throughput direct assay
(Chan et al., 2011)
located_in neuronal cell body
inferred from direct assay
(Jin et al., 2012)
inferred from high throughput direct assay
(Chan et al., 2011)
located_in synapse
inferred from direct assay
(Jin et al., 2012)
Expression Deduced from Reporters
Reporter: PBac{I-SceI(FRT.Rab14-GAL4.ATG(loxP.3xP3-RFP))}
Stage
Tissue/Position (including subcellular localization)
Reference
third instar larval stage
neuron | subset of embryonic/larval brain
(Chan et al., 2011)
glial cell | subset of embryonic/larval brain
(Chan et al., 2011)
embryonic/larval salivary gland | restricted
(Jin et al., 2012, Chan et al., 2011)
neuron | subset of ventral nerve cord
(Chan et al., 2011)
wing disc | restricted
(Jin et al., 2012, Chan et al., 2011)
eye disc | restricted
(Jin et al., 2012, Chan et al., 2011)
ventral thoracic disc | restricted
(Jin et al., 2012, Chan et al., 2011)
neuropil glial sheath
(Jin et al., 2012)
Bolwig organ | restricted
(Jin et al., 2012)
embryonic/larval mushroom body | intense
(Jin et al., 2012)
embryonic/larval optic stalk sheath cell
(Jin et al., 2012)
pupal stage P6 -- P7
neuron | subset of pupal brain

Comment: 30-40h APF

(Chan et al., 2011)
mushroom body

Comment: 30-40h APF

(Chan et al., 2011)
neuropil glial sheath

Comment: 30-40h APF

(Jin et al., 2012)
neuron of adult optic lobe

Comment: 30-40h APF

(Jin et al., 2012)
mushroom body | intense

Comment: 30-40h APF

(Jin et al., 2012)
eye photoreceptor cell | intense

Comment: 30-40h APF

(Jin et al., 2012)
adult stage
neuron | subset of adult brain | ubiquitous
(Chan et al., 2011)
adult mushroom body | intense
(Jin et al., 2012, Chan et al., 2011)
adult ensheathing glial cell
(Jin et al., 2012)
adult inner optic chiasma giant glial cell
(Jin et al., 2012)
neuron of adult optic lobe
(Jin et al., 2012)
adult antennal lobe
(Jin et al., 2012)
adult insulin secreting cell of pars intercerebralis | faint
(Jin et al., 2012)
neuron of lamina
(Jin et al., 2012)
ellipsoid body | intense
(Jin et al., 2012)
fan-shaped body
(Jin et al., 2012)
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Rab14 in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
FLIGHT - Cell culture data for RNAi and other high-throughput technologies
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyBase Wiki
Image Based Resources
Alleles, Insertions, and Transgenic Constructs
Classical and Insertion Alleles ( 6 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 10 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of Rab14
Transgenic constructs containing regulatory region of Rab14
Deletions and Duplications ( 2 )
Phenotypes
Orthologs
Downloads
Download All DIOPT Orthologs
Download All OrthoDB Orthologs
Human Orthologs (via DIOPT v8.0)
Homo sapiens (Human) (3)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Hsap\RAB14
13 of 15
Yes
Yes
Hsap\RAB4A
2 of 15
No
No
Hsap\RAB4B
2 of 15
No
No
Model Organism Orthologs (via DIOPT v8.0)
Mus musculus (laboratory mouse) (3)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Mmus\Rab14
12 of 15
Yes
Yes
Mmus\Rab4a
2 of 15
No
No
Mmus\Rab4b
2 of 15
No
No
Rattus norvegicus (Norway rat) (3)
Rnor\Rab14
12 of 13
Yes
Yes
Rnor\Rab4a
2 of 13
No
No
Rnor\Rab4b
2 of 13
No
No
Xenopus tropicalis (Western clawed frog) (2)
Xtro\rab14
8 of 12
Yes
Yes
Xtro\rab4b
2 of 12
No
No
Danio rerio (Zebrafish) (4)
Drer\rab14
14 of 15
Yes
Yes
Drer\rab14l
9 of 15
No
Yes
Drer\rab4a
2 of 15
No
No
Drer\rab4b
2 of 15
No
No
Caenorhabditis elegans (Nematode, roundworm) (2)
Cele\rab-14
12 of 15
Yes
Yes
Cele\F11A5.4
1 of 15
No
No
Arabidopsis thaliana (thale-cress) (8)
Atha\GB2
1 of 9
Yes
No
Atha\RABA1b
1 of 9
Yes
No
Atha\RABA1c
1 of 9
Yes
No
Atha\RABA1d
1 of 9
Yes
No
Atha\RABA6a
1 of 9
Yes
Yes
Atha\RABA6b
1 of 9
Yes
Yes
Atha\RABB1a
1 of 9
Yes
No
Atha\RABB1C
1 of 9
Yes
No
Saccharomyces cerevisiae (Brewer's yeast) (0)
No records found.
Schizosaccharomyces pombe (Fission yeast) (1)
Spom\ypt4
3 of 12
Yes
No
Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG09190EN8 )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila suzukii
Spotted wing Drosophila
Dsuz\DS10_00000882
Drosophila simulans
Dsim\GD23961
Drosophila sechellia
Dsec\GM15560
Drosophila yakuba
Dyak\GE19031
Drosophila ananassae
Dana\GF14883
Drosophila pseudoobscura pseudoobscura
Dpse\GA18036
Drosophila persimilis
Dper\GL21269
Drosophila willistoni
Dwil\GK18273
Drosophila virilis
Dvir\GJ18183
Drosophila mojavensis
Dmoj\GI12880
Drosophila grimshawi
Dgri\GH11344
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG09150BCI )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Mdoa\17013050
Glossina morsitans
Tsetse fly
Gmor\GMOY004495
Lucilia cuprina
Australian sheep blowfly
Lcup\FF38_02563
Mayetiola destructor
Hessian fly
Mdes\Mdes010671
Aedes aegypti
Yellow fever mosquito
Aaeg\AAEL013252
Anopheles darlingi
American malaria mosquito
Adar\ADAC007617
Anopheles gambiae
Malaria mosquito
Agam\AGAP008419
Culex quinquefasciatus
Southern house mosquito
Cqui\CPIJ004858
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W0CQG )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Bmor\BGIBMGA006221
Bombyx mori
Silkmoth
Bmor\BGIBMGA001928
Bombyx mori
Silkmoth
Bmor\BGIBMGA006641
Danaus plexippus
Monarch butterfly
Dple\DPOGS207506-PA
Danaus plexippus
Monarch butterfly
Dple\DPOGS214204-PA
Danaus plexippus
Monarch butterfly
Dple\DPOGS209180-PA
Heliconius melpomene
Postman butterfly
Hmel\HMEL017959
Heliconius melpomene
Postman butterfly
Hmel\HMEL006820
Apis florea
Little honeybee
Aflo\4039127684
Apis florea
Little honeybee
Aflo\3035667908
Apis mellifera
Western honey bee
Amel\Rab14
Apis mellifera
Western honey bee
Amel\LOC409126
Bombus impatiens
Common eastern bumble bee
Bimp\BIMP10322
Bombus impatiens
Common eastern bumble bee
Bimp\BIMP21731
Bombus terrestris
Buff-tailed bumblebee
Bter\15205436
Bombus terrestris
Buff-tailed bumblebee
Bter\15213018
Linepithema humile
Argentine ant
Lhum\LH20371
Megachile rotundata
Alfalfa leafcutting bee
Mrot\MROTU:00261b
Megachile rotundata
Alfalfa leafcutting bee
Mrot\MROTU:0015bb
Nasonia vitripennis
Parasitic wasp
Nvit\Nasvi2EG014315
Nasonia vitripennis
Parasitic wasp
Nvit\Nasvi2EG008053
Dendroctonus ponderosae
Mountain pine beetle
Dpod\YQE_07225
Tribolium castaneum
Red flour beetle
Tcas\TC010138
Tribolium castaneum
Red flour beetle
Tcas\TC013891
Pediculus humanus
Human body louse
Phum\PHUM517060
Pediculus humanus
Human body louse
Phum\PHUM225030
Pediculus humanus
Human body louse
Phum\PHUM137540
Rhodnius prolixus
Kissing bug
Rpro\RPRC006184
Rhodnius prolixus
Kissing bug
Rpro\RPRC015391
Rhodnius prolixus
Kissing bug
Rpro\RPRC004106
Rhodnius prolixus
Kissing bug
Rpro\RPRC013377
Cimex lectularius
Bed bug
Clec\CLEC013456
Cimex lectularius
Bed bug
Clec\CLEC007120
Cimex lectularius
Bed bug
Clec\CLEC009026
Acyrthosiphon pisum
Pea aphid
Apim\ACYPI000787
Acyrthosiphon pisum
Pea aphid
Apim\ACYPI005434
Zootermopsis nevadensis
Nevada dampwood termite
Znev\Znev_02573
Zootermopsis nevadensis
Nevada dampwood termite
Znev\Znev_10816
Zootermopsis nevadensis
Nevada dampwood termite
Znev\Znev_10541
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X0D9I )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strigamia maritima
European centipede
Smar\SMAR006559
Strigamia maritima
European centipede
Smar\SMAR008992
Ixodes scapularis
Black-legged tick
Isca\ISCW018962
Ixodes scapularis
Black-legged tick
Isca\ISCW020598
Ixodes scapularis
Black-legged tick
Isca\ISCW013805
Stegodyphus mimosarum
African social velvet spider
Smim\KFM62298.1
Stegodyphus mimosarum
African social velvet spider
Smim\KFM63990.1
Stegodyphus mimosarum
African social velvet spider
Smim\KFM80634.1
Tetranychus urticae
Two-spotted spider mite
Turt\tetur14g01590
Tetranychus urticae
Two-spotted spider mite
Turt\tetur09g02960
Tetranychus urticae
Two-spotted spider mite
Turt\tetur14g01950
Daphnia pulex
Water flea
Dpux\DAPPUDRAFT_304738
Daphnia pulex
Water flea
Dpux\DAPPUDRAFT_304304
Daphnia pulex
Water flea
Dpux\DAPPUDRAFT_231914
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G0QOB )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Rab2
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Rab4
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Rab14_1
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Rab14
Ciona intestinalis
Vase tunicate
Cint\LOC100176126
Ciona intestinalis
Vase tunicate
Cint\rab2
Ciona intestinalis
Vase tunicate
Cint\LOC100177325
Gallus gallus
Domestic chicken
Ggal\RAB2A
Gallus gallus
Domestic chicken
Ggal\RAB4A
Gallus gallus
Domestic chicken
Ggal\RAB14
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v8.0)
Drosophila melanogaster (Fruit fly) (34)
Gene Symbol
Score
Source
Compara
eggNOG
Homologene
Inparanoid
Isobase
OrthoDB
OrthoFinder
orthoMCL
Panther
RoundUp
Alignment
Rab4
5 of 10
CG4789
2 of 10
Rab1
2 of 10
Rab2
2 of 10
Rab3
2 of 10
Rab5
2 of 10
Rab6
2 of 10
Rab7
2 of 10
Rab8
2 of 10
Rab9Db
2 of 10
Rab9D
2 of 10
Rab9E
2 of 10
Rab9Fa
2 of 10
Rab9Fb
2 of 10
Rab9
2 of 10
Rab10
2 of 10
Rab11
2 of 10
Rab18
2 of 10
Rab19
2 of 10
Rab21
2 of 10
Rab23
2 of 10
Rab26
2 of 10
Rab27
2 of 10
Rab30
2 of 10
Rab32
2 of 10
Rab35
2 of 10
Rab39
2 of 10
RabX1
2 of 10
RabX2
2 of 10
RabX5
2 of 10
CG15399
1 of 10
Rab40
1 of 10
RabX4
1 of 10
RabX6
1 of 10
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Model Summary Ribbon
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 0 )
    Human Ortholog
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Interaction
    References
    Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    RAB14; RAB14, member RAS oncogene family
    13 of 15
    612673
        Functional Complementation Data
        Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
        Interactions
        Summary of Physical Interactions
        esyN Network Diagram
        Show neighbor-neighbor interactions:
        Select Layout:
        Legend:
        Protein
        RNA
        Selected Interactor(s)
        Interactions Browser
        View in Interactions Browser
        Please see the Physical Interaction reports below for full details
        protein-protein
        Physical Interaction
        Assay
        References
        Rab14 - CG32219
        experimental knowledge based
        (Guruharsha et al., 2011)
        Rab14 - CG6607
        pull down, peptide massfingerprinting
        (Gillingham et al., 2014)
        Rab14 - CG7956
        pull down, peptide massfingerprinting
        (Gillingham et al., 2014, Gillingham et al., 2014)
        Rab14 - CG9590
        pull down, anti tag western blot, two hybrid
        (Gillingham et al., 2014)
        Rab14 - CG9986
        pull down, peptide massfingerprinting
        (Gillingham et al., 2014)
        Rab14 - Dys
        pull down, peptide massfingerprinting
        (Gillingham et al., 2014)
        Rab14 - E(spl)m8-HLH
        experimental knowledge based
        (Guruharsha et al., 2011)
        Rab14 - Evi5
        two hybrid, pull down, anti tag western blot
        (Gillingham et al., 2014)
        Rab14 - Fbp2
        experimental knowledge based
        (Guruharsha et al., 2011)
        Rab14 - Fis1
        pull down, peptide massfingerprinting
        (Gillingham et al., 2014)
        Rab14 - Gdi
        pull down, peptide massfingerprinting
        (Gillingham et al., 2014)
        Rab14 - Klp98A
        anti tag coimmunoprecipitation, anti tag western blot
        (Mauvezin et al., 2016)
        Rab14 - Ocrl
        pull down, peptide massfingerprinting
        (Gillingham et al., 2014)
        Rab14 - Rab7
        experimental knowledge based
        (Guruharsha et al., 2011)
        Rab14 - Rabex-5
        pull down, peptide massfingerprinting
        (Gillingham et al., 2014)
        Rab14 - Rbpn-5
        pull down, peptide massfingerprinting
        (Gillingham et al., 2014)
        Rab14 - RpS15Ab
        experimental knowledge based
        (Guruharsha et al., 2011)
        Rab14 - Tbc1d15-17
        pull down, peptide massfingerprinting
        (Gillingham et al., 2014, Gillingham et al., 2014)
        Rab14 - gsb
        experimental knowledge based
        (Guruharsha et al., 2011)
        Rab14 - pcs
        pull down, peptide massfingerprinting
        (Gillingham et al., 2014)
        Rab14 - trio
        pull down, peptide massfingerprinting
        (Gillingham et al., 2014)
        Rab14 - βTub85D
        experimental knowledge based
        (Guruharsha et al., 2011)
        Summary of Genetic Interactions
        esyN Network Diagram
        Starting gene(s)
        Interaction type
        Interacting gene(s)
        Reference
        Starting gene(s)
        Interaction type
        Interacting gene(s)
        Reference
        External Data
        Linkouts
        DroID - A comprehensive database of gene and protein interactions.
        InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
        MIST (protein-protein) - An integrated Molecular Interaction Database
        Pathways
        Signaling Pathways (FlyBase)
        Metabolic Pathways
        External Data
        Linkouts
        Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
        Genomic Location and Detailed Mapping Data
        Chromosome (arm)
        2L
        Recombination map

        2-50

        Cytogenetic map
        35A4-35A4
        Sequence location
        2L:14,355,145..14,358,764 [+]
        FlyBase Computed Cytological Location
        Cytogenetic map
        Evidence for location
        35A4-35A4
        Limits computationally determined from genome sequence between P{lacW}l(2)34Fak00811&P{PZ}wb09437 and P{EP}elBEP2039&P{PZ}osprJ571
        Experimentally Determined Cytological Location
        Cytogenetic map
        Notes
        References
        35A3-35A4
        (determined by in situ hybridisation)
        (Ashburner et al., 1999, Spradling et al., 1999)
        34F-35A
        (determined by in situ hybridisation)
        (Louis et al., 1997, EDGP Project Members, 1994-)
        Cytological site tentative; in situ of cosmid 57H4 gives repetitive signals.
        (EDGP Project Members, 1994-)
        Experimentally Determined Recombination Data
        Location

        2-50

        (FlyBase, 2016)

        2-46.2

        (Comeron et al., 2012)
        Left of (cM)
        Right of (cM)
        Notes

        Complementation data suggests that l(2)34Fd is distal to Rab14.

        (Ashburner et al., 1999)

        Excisions of Rab14k08712 indicate that Rab14 is between l(2)34Fd and Pgant35A.

        (Ashburner et al., 1999.10.12)
        Stocks and Reagents
        Stocks (17)
        Genomic Clones (14)
         

        Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

        cDNA Clones (125)
         

        Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

        cDNA clones, fully sequenced
        BDGP DGC clones
        Other clones
        Drosophila Genomics Resource Center cDNA clones

        For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

        cDNA Clones, End Sequenced (ESTs)
        BDGP DGC clones
        Other clones
        RNAi and Array Information
        Linkouts
        DRSC - Results frm RNAi screens
        GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
        Antibody Information
        Laboratory Generated Antibodies
         
        Commercially Available Antibodies
         
        Other Information
        Relationship to Other Genes
        Source for database identify of

        Source for identity of: Rab14 CG4212

        (Kodira, 1999.11)
        Source for database merge of

        Source for merge of: Rab14 ESTS:57H4T

        (Ashburner et al., 1999, Ashburner et al., 1999.10.12)
        Additional comments
        Other Comments

        Rab14 positively regulates phagosome maturation. Rab14 regulates fusion of phagosomes with late endosomes and lysosomes.

        (Garg and Wu, 2014)

        dsRNA has been made from templates generated with primers directed against this gene.

        (Kouranti et al., 2006)

        dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.

        (Kiger et al., 2003)

        Data suggests that Rab14 is not a vital gene.

        (Ashburner et al., 1999)

        Isolated from a Drosophila head cDNA library.

        (Satoh et al., 1997)

        This gene was first recognised by a match of an STS sequence from a European Drosophila Genome Mapping Project cosmid.

        Origin and Etymology
        Discoverer
        Etymology
        Identification
        External Crossreferences and Linkouts ( 58 )
        Sequence Crossreferences
        NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
        GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
        GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
        RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
        UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
        Other crossreferences
        Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
        Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
        Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
        Flygut - An atlas of the Drosophila adult midgut
        GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
        iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
        InterPro - A database of protein families, domains and functional sites
        KEGG Genes - Molecular building blocks of life in the genomic space.
        modMine - A data warehouse for the modENCODE project
        Linkouts
        DPiM - Drosophila Protein interaction map
        DroID - A comprehensive database of gene and protein interactions.
        DRSC - Results frm RNAi screens
        FLIGHT - Cell culture data for RNAi and other high-throughput technologies
        FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
        FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
        FlyMine - An integrated database for Drosophila genomics
        InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
        MIST (protein-protein) - An integrated Molecular Interaction Database
        Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
        Synonyms and Secondary IDs (14)
        Datasets (1)
        Study focus (1)
        Experimental Role
        Project
        Project Type
        Title
        • bait_protein
        Rab_Effectors_interaction_map
        Interaction map generated by purification of most Rab factors, with identification of copurifying effectors by mass spectrometry.
        References (93)