FlyBase Gene Report: Dmel\RtGEF

General Information

Symbol

Dmel\RtGEF

Species

D. melanogaster

Name

Rho-type guanine nucleotide exchange factor

Annotation Symbol

CG10043

Feature Type

protein_coding_gene

FlyBase ID

FBgn0015803

Gene Model Status

Current

Stock Availability

17 publicly available

Gene Summary

Rho-type guanine nucleotide exchange factor (RtGEF) encodes a putative Rho-type guanine nucleotide exchange factor for the GTPases encoded by Rac1 and Cdc42. It signals through the sterile-20 kinases encoded by Pak and hpo. The product of RtGEF contributes to regulation of postsynaptic structure, post synaptic muscle development, protein localization and the growth of epithelial tissue. [Date last reviewed: 2019-03-14] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Pathway
Gene Group
Interactive Fly

Also Known As

dpix, 38C.40, pix, rhoGEF, β-PIX

Key Links

Genomic Location

Cytogenetic map

38C5-38C5

Sequence location

2L:20,350,434..20,365,254 [+]

Recombination map

2-54

RefSeq locus

NT_033779 REGION:20350434..20365254

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (7 terms)

Molecular Function (2 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

enables molecular adaptor activity

inferred from mutant phenotype

(Dent et al., 2015)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

enables guanyl-nucleotide exchange factor activity

inferred from sequence or structural similarity with UniProtKB:Q14155

(Gene Ontology Curators, 2002-)

inferred from electronic annotation with InterPro:IPR000219

(InterPro Project Members, 2004-)

inferred from biological aspect of ancestor with PANTHER:PTN001106899

(GO Reference Genome Project, 2011-)

Biological Process (3 terms)

Terms Based on Experimental Evidence (3 terms)

CV Term

Evidence

References

involved_in negative regulation of organ growth

inferred from genetic interaction with FLYBASE:Git; FB:FBgn0033539

(Dent et al., 2015)

inferred from genetic interaction with FLYBASE:yki; FB:FBgn0034970

(Dent et al., 2015)

inferred from genetic interaction with FLYBASE:hpo; FB:FBgn0261456

(Dent et al., 2015)

involved_in positive regulation of hippo signaling

inferred from mutant phenotype

(Portela et al., 2024)

inferred from genetic interaction with FLYBASE:yki; FB:FBgn0034970

(Dent et al., 2015)

inferred from genetic interaction with FLYBASE:hpo; FB:FBgn0261456

(Dent et al., 2015)

involved_in regulation of synapse organization

inferred from mutant phenotype

(Parnas et al., 2001)

Terms Based on Predictions or Assertions (0 terms)

Cellular Component (2 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

located_in muscle cell postsynaptic specialization

inferred from direct assay

(Parnas et al., 2001)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

is_active_in cytoplasm

inferred from biological aspect of ancestor with PANTHER:PTN001106899

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

RHO GUANINE NUCLEOTIDE EXCHANGE FACTORS

Pathway (FlyBase)

POSITIVE REGULATORS OF HIPPO SIGNALING PATHWAY

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

Gene Group (FlyBase)

RHO GUANINE NUCLEOTIDE EXCHANGE FACTORS -: RhoGEFs are Guanine Nucleotide Exchange Factors (GEFs) for Rho family GTPases. They include Dbl-homology domain-containing proteins (the largest family of RhoGEFs) and CZH2 domain/Dock family GEFs. (Adapted from FBrf0190957).

Pathway (FlyBase)

POSITIVE REGULATORS OF HIPPO SIGNALING PATHWAY -: The Hippo signaling pathway is an intracellular kinase cascade in which hpo kinase in complex with sav, phosphorylates wts kinase which, in turn, phosphorylates yki transcriptional co-activator leading to its cytosolic retention. Positive regulators of the pathway, enhance the cytosolic retention of yki, negatively regulating tissue growth (Adapted from FBrf0224870).

Summary (Interactive Fly)

RhoGEF - homolog of mammalian Pix - regulates postsynaptic structure and protein localization at the glutamatergic neuromuscular junction

Interactive Fly

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\RtGEF for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry A8DZ19)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Alternative translation stop created by use of multiphasic reading frames within coding region.

Annotated transcripts do not represent all supported alternative splices within 5' UTR.

Low-frequency RNA-Seq exon junction(s) not annotated.

Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.

Gene model reviewed during 5.48

Gene model reviewed during 6.32

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

RtGEF-RA

FBtr0081356

NM_057826

3336

687

RtGEF-RB

FBtr0081357

NM_165327

2976

687

RtGEF-RD

FBtr0112885

NM_001103715

4194

973

RtGEF-RE

FBtr0302106

NM_001169550

2995

635

RtGEF-RF

FBtr0336747

NM_001103714

5284

1310

RtGEF-RG

FBtr0336748

NM_001273693

3938

923

RtGEF-RH

FBtr0336749

NM_001273694

4343

1198

RtGEF-RI

FBtr0479856

NM_001382006

4943

1310

Additional Transcript Data and Comments

Reported size (kB)

3.8 (northern blot)

(Werner and Manseau, 1997)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

RtGEF-PA

FBpp0080888

75.0

687

6.84

FBpp0080889

75.0

687

6.84

FBpp0111798

106.1

973

8.50

FBpp0291316

69.1

635

7.21

FBpp0307723

144.4

1310

6.31

FBpp0307724

100.5

923

8.95

FBpp0307725

133.1

1198

5.45

FBpp0428192

144.4

1310

6.31

A8DZ19

QJC20915

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

687 aa isoforms: RtGEF-PA, RtGEF-PB

1310 aa isoforms: RtGEF-PF, RtGEF-PI

Additional Polypeptide Data and Comments

Reported size (kDa)

687 (aa)

(Werner and Manseau, 1997)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\RtGEF using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-1.08

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 5

(Werner and Manseau, 1997)

organism | ubiquitous

(Werner and Manseau, 1997)

embryonic stage 6 -- 9

ventral furrow

(Werner and Manseau, 1997)

amnioproctodeal invagination

(Werner and Manseau, 1997)

posterior plate of cibarium

(Werner and Manseau, 1997)

cephalic furrow

(Werner and Manseau, 1997)

stomodeal invagination

(Werner and Manseau, 1997)

embryonic stage 7 -- 8

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 9 -- 10

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 10 -- 11

mesoderm

(Werner and Manseau, 1997)

embryonic stage 11

anterior midgut primordium

(Werner and Manseau, 1997)

posterior midgut primordium

(Werner and Manseau, 1997)

inclusive hindgut primordium

(Werner and Manseau, 1997)

neuroblast | ventral

(Werner and Manseau, 1997)

embryonic stage 11 -- 12

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 12 -- 15

larval digestive system

(Werner and Manseau, 1997)

larval ventral nerve cord

(Werner and Manseau, 1997)

embryonic stage 13 -- 16

organism | ubiquitous

(Fisher et al., 2012)

prepupal stage P3

leg | ubiquitous

(Greenberg and Hatini, 2011)

adult stage & oogenesis

follicle cell

(Werner and Manseau, 1997)

nurse cell

(Werner and Manseau, 1997)

adult stage oogenesis stage S1 -- S8

oocyte

(Werner and Manseau, 1997)

Additional Descriptive Data

Expression in stage P3 pupal leg discs is ubiquitous.

(Greenberg and Hatini, 2011)

The RtGEF transcript is detected throughout oogenesis in nurse and follicle cells, and in the oocyte up to stage S9. During early embryogenesis, the RtGEF transcript is ubiquitous, but at gastrulation it is most abundant in or near cells involved in morphogenetic movements, such as the ventral and cephalic furrows, the posterior plate, and the midgut invaginations. Ventral neuroblasts express RtGEF at stage 11, and the transcript is detected along the ventral nerve cord at stages 12 to 15. At stage 11, RtGEF transcript is detecte in the anterior and posterior midgut, and the hindgut, and gut staining persists through stages 12-15.

(Werner and Manseau, 1997)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in muscle cell postsynaptic specialization

inferred from direct assay

(Parnas et al., 2001)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\RtGEF in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\RtGEF

View exonic expression by tissue for Dmel\RtGEF

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0015803

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

35306/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0015803

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0015803

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0015803

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG10043

Flygut - An atlas of the Drosophila adult midgut

FBgn0015803

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 14 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 22 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of RtGEF

Transgenic constructs containing regulatory region of RtGEF

Aberrations (Deficiencies and Duplications) ( 4 )

Inferred from experimentation ( 4 )

Gene disrupted in

Df(2L)Exel6046

(Ryder, 2004.4.26)

Df(2L)PJ2

(Bai and Tolias, 2000)

Df(2L)PJ19

(Parnas et al., 2001, Bai and Tolias, 2000)

Gene not disrupted in

Df(2L)PJ17

(Parnas et al., 2001)

Inferred from location ( 6 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

partially lethal - majority die (with Df(2L)PJ19)

RtGEF^p1036

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

RtGEF^GD6845

viable, with Scer\GAL4^Mef2.PR

RtGEF^GD6845

viable, with Scer\GAL4^pnr-MD237

RtGEF^GD6845

viable, with Scer\GAL4^tin.CΔ4

RtGEF^GD6845

viable (with Df(2L)PJ19)

Other Phenotypes

Allele

abnormal neuroanatomy

RtGEF¹

abnormal neuroanatomy (with Df(2L)PJ19)

abnormal neuroanatomy (with RtGEF¹)

RtGEF²

abnormal neuroanatomy (with RtGEF²)

RtGEF¹

abnormal neurophysiology (with Df(2L)PJ19)

Phenotype manifest in

Allele

NMJ bouton

RtGEF¹

NMJ bouton (with Df(2L)PJ19)

RtGEF^p1036

postsynaptic membrane (with Df(2L)PJ19)

RtGEF^p1036

postsynaptic membrane (with RtGEF¹)

RtGEF²

postsynaptic membrane (with RtGEF²)

RtGEF¹

synaptic vesicle (with Df(2L)PJ19)

t-bar (with Df(2L)PJ19)

RtGEF^p1036

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (53)

Hsap\ARHGEF6

8 of 14

Yes

Hsap\ARHGEF7

8 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (34)

Rnor\Arhgef6

8 of 14

Yes

Rnor\Arhgef7

8 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Rnor\Dnmbp

1 of 14

Rnor\Ect2l

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Mus musculus (laboratory mouse) (32)

Mmus\Arhgef6

8 of 14

Yes

Mmus\Arhgef7

8 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Xenopus tropicalis (Western clawed frog) (20)

Xtro\arhgef6

7 of 13

Yes

Xtro\arhgef7

7 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Xtro\plekhg2

1 of 13

Xtro\plekhg4

1 of 13

Xtro\prex1

1 of 13

Yes

Xtro\prex2

1 of 13

Xtro\sos2

1 of 13

Xtro\trio

1 of 13

Danio rerio (Zebrafish) (35)

Drer\arhgef6

8 of 14

Yes

Drer\arhgef7a

8 of 14

Yes

Drer\arhgef7b

8 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (7)

Cele\pix-1

9 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Anopheles gambiae (African malaria mosquito) (12)

Agam\AgaP_AGAP007877

9 of 12

Yes

Agam\AgaP_AGAP006382

2 of 12

Agam\AgaP_AGAP001238

1 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (0)

Saccharomyces cerevisiae (Brewer's yeast) (3)

Scer\CDC24

1 of 13

Yes

Scer\GGA2

1 of 13

Yes

Scer\HSE1

1 of 13

Yes

Schizosaccharomyces pombe (Fission yeast) (3)

Spom\hse1

1 of 12

Yes

Spom\scd1

1 of 12

Yes

Spom\SPAC1F3.05

1 of 12

Yes

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:RtGEF. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (14)

CG30440

2 of 13

GEFmeso

2 of 13

Pura

2 of 13

sif

2 of 13

trio

2 of 13

Vav

2 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

ARHGEF6; Rac/Cdc42 guanine nucleotide exchange factor 6

8 of 14

300267

ARHGEF7; Rho guanine nucleotide exchange factor 7

8 of 14

605477

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

RtGEF - Git

experimental knowledge based, anti tag coimmunoprecipitation, anti tag western blot

(Dent et al., 2015, Guruharsha et al., 2011, Bahri et al., 2009)

RtGEF - Pi3K92E

anti tag coimmunoprecipitation, peptide massfingerprinting

(Glatter et al., 2011)

RtGEF - hpo

anti tag coimmunoprecipitation, anti tag western blot, peptide massfingerprinting

(Dent et al., 2015)

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

RtGEF|yki

suppressible

(Dent et al., 2015)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

hpo

enhanceable

RtGEF|Git

(Dent et al., 2015)

hpo

enhanceable

RtGEF

(Dent et al., 2015)

yki

enhanceable

RtGEF

(Dent et al., 2015)

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

61272

DroID - A comprehensive database of gene and protein interactions.

FBgn0015803

MIST (protein-protein) - An integrated Molecular Interaction Database

35306

Pathways

Signaling Pathways (FlyBase)

POSITIVE REGULATORS OF HIPPO SIGNALING PATHWAY

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

SignaLink - A signaling pathway resource with multi-layered regulatory networks.

A8DZ19

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-54

Cytogenetic map

38C5-38C5

Sequence location

2L:20,350,434..20,365,254 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

38C5-38C5

Limits computationally determined from genome sequence between P{lacW}k09005 and P{EP}CG16798^EP401&P{lacW}k07219

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

38C-38C

(Pinter, 1998.3.9, Werner, 1996.4.28)

Experimentally Determined Recombination Data

Location

2-54

(FlyBase, 2016)

2-54.0

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (17)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

604918

w^*; TI{TI}RtGEF^Venus

29166

w¹¹¹⁸; Mi{ET1}RtGEF^MB10902

27123

y¹ w^*; P{EP}RtGEF^G3647

77656

w¹¹¹⁸; PBac{IT.GAL4}RtGEF^4046-G4/CyO

53466

y¹ w^*; Mi{MIC}RtGEF^MI10186/SM6a

Kyoto

104218

w^*; P{GawB}RtGEF^NP2491 / CyO

NIG-Fly

10043R-3

P{NIG.10043R}3

M2L-3353

y¹ w¹¹¹⁸; RtGEF^SK13 P{neoFRT}40A/CyO-Tb

M2L-3354

y¹ w¹¹¹⁸; RtGEF^SK14 P{neoFRT}40A/CyO-Tb

VDRC

v100583

P{KK108300}VIE-260B

v105093

P{KK113571}VIE-260B

v17966

w¹¹¹⁸; P{GD6845}v17966

More stocks available...

Genomic Clones (13)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (97)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0015803

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0015803

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Parnas et al., 2001)

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

RtGEF and Git regulate hpo-pathway dependent tissue growth. They appear to influence the activity of the hpo kinase by acting as a scaffold complex, promoting hpo dimerisation and autophosphorylation of hpo's activation loop.

(Dent et al., 2015)

One of 42 Drosophila genes identified as being most likely to reveal molecular and cellular mechanisms of nervous system development or plasticity relevant to human Mental Retardation disorders.

(Inlow and Restifo, 2004)

dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R⁺ cell morphology.

(Kiger et al., 2003)

rtGEF is necessary for correct postsynaptic structure and assembly of postsynaptic proteins.

(Parnas et al., 2001)

Encodes a guanine nucleotide exchange factor. Expressed throughout oogenesis and embryogenesis and in the embryo mRNA is abundant in cells undergoing morphogenetic movements.

(Werner and Manseau, 1997)

"RhoGEF" gene symbol is temporary.

(Werner, 1996.4.28)

Relationship to Other Genes

Source for database merge of

Additional comments

Nomenclature History

Source for database identify of

Source for identity of: rtGEF CG10043

(Lasko, 1999.11)

Source for identity of: RtGEF rtGEF

(FlyBase, 2009-)

Nomenclature comments

Etymology

Synonyms and Secondary IDs (21)

Reported As

Symbol Synonym

38C.40

(Butler, 2001, Butler et al., 2001, McGill Drosophila Genome Project, 2001.9.25)

ARHGEF7

(Garcia-Mata and Burridge, 2007)

CG10043

(National Institute of Genetics Fly Stocks, 2022-, Glatter et al., 2011, Greenberg and Hatini, 2011, Ni et al., 2010.12.1, Keleman et al., 2009.8.5)

DrhoGEF

(Hicks et al., 2001)

DrtGEF

(Werner and Manseau, 1997)

Pix

(Dent et al., 2015, Parnas et al., 2001)

Rho/RacGEF

(Werner et al., 1996)

RhoGEF

(Werner, 1996.4.28)

RtGEF

(Durel et al., 2025, di Pietro et al., 2023, Ho and Treisman, 2020, Treisman, 2019.8.23, Clandinin and Owens, 2016-, Dent et al., 2015, Kavi et al., 2015, Steele et al., 2015)

dPIX

(Bahri et al., 2009)

dPix

(Bischoff et al., 2025, Ho and Treisman, 2020, Lee and Schwarz, 2016, Mosca and Schwarz, 2010, Rohrbough et al., 2007)

dpix

(Dent et al., 2019, Rui et al., 2017, Bahri et al., 2010, Bahri et al., 2009, Parnas et al., 2001)

pix

(Dent et al., 2015, Ramos et al., 2015)

rhoGEF

(Zhu et al., 2002, Bai and Tolias, 2000)

rtGEF

(Lindsey et al., 2021, Ashwal-Fluss et al., 2014, Das et al., 2013, Japanese National Institute of Genetics, 2012.5.21, Friedman et al., 2011, Greenberg and Hatini, 2011, Bakal et al., 2007)

β-PIX

(Stephens et al., 2018, Garcia-Mata and Burridge, 2007)

βPIX

(Bahri et al., 2010)

Name Synonyms

Drosophila rho-type guanine exchange factor

(Werner and Manseau, 1997)

PAK-interacting exchange factor

(Dent et al., 2015)

Rho-type guanine nucleotide exchange factor

rho-type guanine exchange factor

(Werner and Manseau, 1997)

Secondary FlyBase IDs

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 84 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

35306

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

A8DZ19

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

A8DZ19

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0015803

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

35306/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0015803

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0015803

FlyMine - An integrated database for Drosophila genomics

FBgn0015803

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG10043

MARRVEL_MODEL - MARRVEL (model organism gene)

35306

Linkouts

BioGRID - A database of protein and genetic interactions.

61272

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0015803

DroID - A comprehensive database of gene and protein interactions.

FBgn0015803

DRSC - Results frm RNAi screens

FBgn0015803

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0015803

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0015803

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG10043

Flygut - An atlas of the Drosophila adult midgut

FBgn0015803

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0015803

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC014788

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

MIST (protein-protein) - An integrated Molecular Interaction Database

35306

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

SignaLink - A signaling pathway resource with multi-layered regulatory networks.

A8DZ19

References (79)

The following external sites may use different assemblies or annotations than FlyBase.

-1.08

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