FlyBase Gene Report: Dmel\Pdp1

adult head

(Kadener et al., 2006)

adult brain

Comment: Pdp1 mRNA levels do not significantly differ between ZT4 and ZT16.

(Kadener et al., 2006)

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

posterior ectoderm anlage

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

Comment: anlage in statu nascendi

(Fisher et al., 2012)

embryonic stage 7 -- 8

posterior endoderm

(Fisher et al., 2012)

embryonic stage 9 -- 10

anterior endoderm

(Fisher et al., 2012)

posterior endoderm

(Fisher et al., 2012)

embryonic stage 9 -- 11

posterior endoderm

(Lin et al., 1997)

embryonic stage 9 -- 17

anterior midgut primordium

Comment: extended 3' UTR isoform

(Hilgers et al., 2011)

embryonic stage 11

(Lin et al., 1997)

head mesoderm

(Lin et al., 1997)

posterior midgut primordium

(Reddy et al., 2000)

embryonic stage 11 -- 12

anterior midgut primordium

(Fisher et al., 2012, Reddy et al., 2000)

anal pad primordium

Comment: reported as anal pad specific anlage

(Fisher et al., 2012)

posterior midgut primordium

(Fisher et al., 2012)

embryonic stage 12 -- 14

fused midgut primordium

(Reddy et al., 2000)

embryonic Malpighian tubule

(Reddy et al., 2000)

somatic muscle primordium

(Reddy et al., 2000)

dorsal pharyngeal muscle primordium

(Reddy et al., 2000)

embryonic hindgut

(Reddy et al., 2000)

embryonic stage 13

myoblast

(Lin et al., 1997)

embryonic stage 13 -- 14

presumptive embryonic/larval central nervous system

(Reddy et al., 2000)

embryonic stage 13 -- 16

dorsal pharyngeal muscle cell

(Fisher et al., 2012)

embryonic anal pad

(Fisher et al., 2012)

embryonic/larval midgut

(Fisher et al., 2012)

larval muscle system

(Fisher et al., 2012)

embryonic/larval esophageal visceral muscle

(Fisher et al., 2012)

embryonic stage 14

pharyngeal muscle cell

(Lin et al., 1997)

somatic muscle cell

(Lin et al., 1997)

endoderm of midgut primordium

(Lin et al., 1997)

epithelium of hindgut

(Lin et al., 1997)

fat body primordium

(Reddy et al., 2000, Lin et al., 1997)

embryonic midgut constriction 2

(Reddy et al., 2000)

embryonic stage 14 -- 15

gastric caecum primordium

(Reddy et al., 2000)

embryonic stage 15

embryonic epidermis | segmentally repeated

(Lin et al., 1997)

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 17

Comment: 0-21 hr AEL

(Lin et al., 1997)

adult stage | female

ovary

(Lin et al., 1997)

RT-PCR

Stage

Tissue/Position (including subcellular localization)

Reference

adult head

Comment: expression specific to isoforms Pdp1-RD, RJ

(Zheng et al., 2009)

adult stage | male

anterior midgut primordium

Comment: circadian oscillating expression

(Rakshit et al., 2012)

Additional Descriptive Data

Expression oscillates with the circadian cycle and peaks at ZT16, with the peak becoming less pronounced as flies age. The relative amount of mRNA is less in bodies compared to heads.

(Rakshit et al., 2012)

Zygotic-specific isoforms of Pdp1 with long 3' UTR extensions were observed.

(Hilgers et al., 2011)

The Pdp1 isoforms Pdp1-RD and RJ, collectively referred in this paper as Pdp1-ε isoform, are detected in the heads of adult flies by RT-PCR. In conditions of constant darkness, the levels of both isoforms cycle, increasing gradually from CT02 until CT14, and decreasing after that until CT20.

(Zheng et al., 2009)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 10 -- 11

(Reddy et al., 2000)

posterior midgut primordium

(Reddy et al., 2000)

somatic trunk mesoderm | segmentally repeated of germ band

(Reddy et al., 2000)

head mesoderm

(Reddy et al., 2000)

embryonic stage 13

embryonic hindgut

(Reddy et al., 2000)

embryonic Malpighian tubule

(Reddy et al., 2000)

embryonic stage 14

interstitial cell precursor

(Reddy et al., 2000)

somatic muscle primordium

(Reddy et al., 2000)

fat body primordium

(Reddy et al., 2000)

embryonic stage 14 -- 15

fused midgut primordium | posterior

(Reddy et al., 2000)

gastric caecum primordium

(Reddy et al., 2000)

embryonic esophagus

(Reddy et al., 2000)

embryonic stage 15

pharyngeal muscle cell

(Reddy et al., 2000)

embryonic stage 15 -- 16

larval central nervous system

• nucleus

(Reddy et al., 2000)

embryonic stage 16

embryonic/larval fat body

• nucleus

(Reddy et al., 2000)

gustatory receptor neuron of the labellum

adult s-LNv neuron

(Nitabach et al., 2006)

LNd neuron

Comment: at ZT20

(Zheng et al., 2009, Nitabach et al., 2006, Shafer et al., 2006)

DN1 neuron

(Nitabach et al., 2006)

DN2 neuron

(Nitabach et al., 2006)

DN1p neuron

(Shafer et al., 2006)

LNv neuron

Comment: at ZT20

(Zheng et al., 2009)

(Chatterjee et al., 2010)

western blot

Stage

Tissue/Position (including subcellular localization)

Reference

View Dmel\Pdp1 in JBrowse

adult head

Comment: expression specific to isoforms Pdp1-PD, PJ

(Zheng et al., 2009)

Additional Descriptive Data

The Pdp1 isoforms Pdp1-PD and PD, collectively referred in this paper as Pdp1-ε isoform, are detected in head extracts of adult flies by western blot. The isoform Pdp1-PJ is the predominant one. Both isoforms show a cycling behaviour, with levels gradually increasing from CT02 until CT18, and decrease after that until ZT22. Immunostaining reveals that at ZT20, Pdp1 protein is expressed in the dorsal and ventral lateral clock neurons (LNd and LNv) in the adult brain.

(Zheng et al., 2009)

Pdp1 is predominantly nuclear at ZT18.

(Shafer et al., 2006)

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in nucleus

inferred from direct assay

(Reddy et al., 2000)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Pdp1

View exonic expression by tissue for Dmel\Pdp1

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0016694

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

45588/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0016694

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0016694

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0016694

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG17888

Flygut - An atlas of the Drosophila adult midgut

FBgn0016694

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 33 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 11 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Pdp1

Transgenic constructs containing regulatory region of Pdp1

Aberrations (Deficiencies and Duplications) ( 5 )

Inferred from experimentation ( 5 )

Gene disrupted in

Df(3L)BSC375

(Christensen et al., 2007.10.29)

Df(3L)BSC388

(Christensen et al., 2007.10.29)

Df(3L)BSC459

(Christensen et al., 2008.4.15)

Df(3L)BSC631

(Christensen et al., 2008.10.20)

Df(3L)BSC807

(Christensen et al., 2009.6.15)

Inferred from location ( 7 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal, with Scer\GAL4^pnr-MD237

Pdp1^GD4606

lethal | larval stage

Pdp1^qq

lethal | pupal stage

Pdp1^qq

lethal | recessive

Pdp1^P205

lethal - all die before end of larval stage | recessive

Pdp1^P205

viable

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Pdp1^GD4606

viable, with Scer\GAL4^tin.CΔ4

Pdp1^GD4606

Sterility

Allele

fertile

Pdp1^EY14882

Other Phenotypes

Allele

abnormal circadian behavior (with Pdp1³¹³⁵)

Pdp1^d11071

abnormal circadian behavior (with Pdp1^d11071)

Pdp1³¹³⁵

abnormal circadian behavior | recessive

abnormal developmental rate

Pdp1^qq

abnormal developmental rate | recessive

Pdp1^P205

decreased body size

Pdp1^qq

decreased body size | larval stage | recessive

Pdp1^P205

decreased occurrence of cell division | larval stage

Pdp1^P205

flightless, with Scer\GAL4^Mef2.PR

Pdp1^GD4606

some die during larval stage, with Scer\GAL4^da.PU

Pdp1^RNAi.WIZ.UAS

some die during larval stage | recessive

Pdp1^P205

some die during pupal stage, with Scer\GAL4^da.PU

Pdp1^RNAi.WIZ.UAS

visible, with Scer\GAL4^Bx-MS1096

visible | cold sensitive, with Scer\GAL4^Bx-MS1096

Pdp1^{UAS.ORF.GW.Tag:HA}

visible | male limited, with Scer\GAL4^Bx-MS1096

Pdp1^KK109014

Phenotype manifest in

Allele

M band, with Scer\GAL4^Mef2.PR

Pdp1^GD4606

myofibril, with Scer\GAL4^Mef2.PR

Pdp1^GD4606

sarcomere, with Scer\GAL4^Mef2.PR

Pdp1^GD4606

wing, with Scer\GAL4^Bx-MS1096

wing | cold sensitive, with Scer\GAL4^Bx-MS1096

Pdp1^{UAS.ORF.GW.Tag:HA}

wing | male limited, with Scer\GAL4^Bx-MS1096

Pdp1^KK109014

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (32)

Hsap\HLF

11 of 14

Yes

Hsap\TEF

9 of 14

Yes

Hsap\DBP

8 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

Yes

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (27)

Rnor\Hlf

10 of 14

Yes

Rnor\Tef

9 of 14

Yes

Rnor\Dbp

8 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

Yes

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Mus musculus (laboratory mouse) (28)

Mmus\Hlf

11 of 14

Yes

Mmus\Tef

9 of 14

Yes

Mmus\Dbp

7 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

Yes

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Xenopus tropicalis (Western clawed frog) (24)

Xtro\hlf

8 of 13

Yes

Xtro\tef

7 of 13

Yes

Xtro\dbp

5 of 13

Yes

Xtro\tap1

4 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Xtro\tfap2c

1 of 13

Xtro\thibz

1 of 13

Xtro\xbp1

1 of 13

Danio rerio (Zebrafish) (39)

Drer\hlfa

11 of 14

Yes

Drer\hlfb

10 of 14

Yes

Drer\dbpa

9 of 14

Yes

Drer\dbpb

9 of 14

Yes

Drer\tefa

9 of 14

Yes

Drer\tefb

8 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Drer\ddit3

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Drer\si:ch211-153j24.3

1 of 14

Drer\si:dkey-172o19.2

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (10)

Cele\atf-8

4 of 14

Yes

Cele\ces-2

4 of 14

Yes

Cele\zip-7

3 of 14

Yes

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Cele\zip-8

1 of 14

Yes

Anopheles gambiae (African malaria mosquito) (8)

Agam\AgaP_AGAP006376

8 of 12

Yes

2 of 12

2 of 12

2 of 12

2 of 12

1 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (15)

Atha\ABF1

1 of 13

Yes

Atha\ABF3

1 of 13

Yes

Atha\ABI5

1 of 13

Yes

Atha\AT5G07160

1 of 13

Yes

Atha\bZIP5

1 of 13

Yes

Atha\BZIP9

1 of 13

Yes

Atha\bZIP16

1 of 13

Yes

Atha\BZIP28

1 of 13

Yes

Atha\BZIP34

1 of 13

Yes

Atha\BZIP61

1 of 13

Yes

Atha\bZIP68

1 of 13

Yes

Atha\bZIP70

1 of 13

Yes

Atha\GBF1

1 of 13

Yes

Atha\GBF3

1 of 13

Yes

Atha\HYH

1 of 13

Yes

Saccharomyces cerevisiae (Brewer's yeast) (1)

Scer\ACA1

1 of 13

Yes

Schizosaccharomyces pombe (Fission yeast) (1)

Spom\atf1

1 of 12

Yes

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Pdp1. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (10)

CG7786

4 of 13

3 of 13

Irbp18

2 of 13

slbo

2 of 13

vri

2 of 13

Atf3

1 of 13

crc

1 of 13

CrebB

1 of 13

Jra

1 of 13

kay

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Allele

Disease

Interaction

References

Pdp1^RNAi.WIZ.UAS

exacerbates tauopathy

modeled by Hsap\MAPT^UAS.cWa

(Feuillette et al., 2020)

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

HLF; HLF transcription factor, PAR bZIP family member

11 of 14

142385

TEF; TEF transcription factor, PAR bZIP family member

9 of 14

188595

DBP; D-box binding PAR bZIP transcription factor

8 of 14

124097

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

RNA-protein

Physical Interaction

Assay

References

Pdp1 - Atx2

anti tag coimmunoprecipitation, primer specific pcr, quantitative reverse transcription pcr

(Lee et al., 2017, Lim and Allada, 2013)

Pdp1 - tyf

anti tag coimmunoprecipitation, primer specific pcr

(Lim and Allada, 2013)

protein-protein

Physical Interaction

Assay

References

Pdp1

fluorescent resonance energy transfer

(Reinke et al., 2013)

Pdp1 - Bro

(Shokri et al., 2019)

Pdp1 - BuGZ

(Shokri et al., 2019)

Pdp1 - CG4328

(Shokri et al., 2019)

Pdp1 - Mondo

enzymatic study, autoradiography, anti tag coimmunoprecipitation, anti tag western blot

(Shokri et al., 2019)

Pdp1 - dco

(Choi et al., 2009)

Pdp1 - fd3F