FlyBase Gene Report: Dmel\ari-1

General Information

Symbol

Dmel\ari-1

Species

D. melanogaster

Name

ariadne 1

Annotation Symbol

CG5659

Feature Type

protein_coding_gene

FlyBase ID

FBgn0017418

Gene Model Status

Current

Stock Availability

35 publicly available

Enzyme Name (EC)

Gene Summary

ariadne 1 (ari-1) encodes an E3 ubiquitin ligase that interacts with the E2 ubiquitin conjugating enzyme encoded by Ubc10. The product of ari-1 and Ubc10 jointly ubiquitylate isoform A of the ecdysone receptor encoded by EcR. [Date last reviewed: 2019-03-07] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
UniProtKB
Interactive Fly

Also Known As

ari, ariadne, HL-V

Key Links

Genomic Location

Cytogenetic map

16F7-16F7

Sequence location

X:18,130,392..18,137,784 [-]

Recombination map

1-59

RefSeq locus

NC_004354 REGION:18130392..18137784

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (16 terms)

Molecular Function (5 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

enables ubiquitin conjugating enzyme binding

inferred from physical interaction with FLYBASE:Ubc10; FB:FBgn0026316

(Tan et al., 2018, Aguilera et al., 2000)

enables ubiquitin protein ligase activity

inferred from direct assay

(Gradilla et al., 2011)

inferred from mutant phenotype

(Tan et al., 2018)

Terms Based on Predictions or Assertions (5 terms)

CV Term

Evidence

References

enables metal ion binding

inferred from electronic annotation with InterPro:IPR018957

(InterPro Project Members, 2004-)

enables ubiquitin conjugating enzyme binding

inferred from biological aspect of ancestor with PANTHER:PTN000188395

(GO Reference Genome Project, 2011-)

enables ubiquitin protein ligase activity

inferred from biological aspect of ancestor with PANTHER:PTN000188395

(GO Reference Genome Project, 2011-)

enables ubiquitin-protein transferase activity

inferred from electronic annotation with InterPro:IPR031127

(InterPro Project Members, 2004-)

enables zinc ion binding

inferred from sequence model

(Ying et al., 2011)

inferred from electronic annotation with InterPro:IPR002867

(InterPro Project Members, 2004-)

Biological Process (7 terms)

Terms Based on Experimental Evidence (7 terms)

CV Term

Evidence

References

involved_in endoplasmic reticulum organization

inferred from mutant phenotype

(Aguilera et al., 2000)

involved_in oogenesis

inferred from mutant phenotype

(Aguilera et al., 2000)

involved_in positive regulation of neurogenesis

inferred from mutant phenotype

(Aguilera et al., 2000)

involved_in positive regulation of proteasomal protein catabolic process

inferred from mutant phenotype

(Tan et al., 2018)

involved_in protein monoubiquitination

inferred from direct assay

(Tan et al., 2018)

involved_in protein ubiquitination

inferred from direct assay

(Gradilla et al., 2011)

involved_in ubiquitin-dependent protein catabolic process

inferred from mutant phenotype

(Gradilla et al., 2011)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

involved_in protein ubiquitination

inferred from electronic annotation with InterPro:IPR031127

(InterPro Project Members, 2004-)

involved_in ubiquitin-dependent protein catabolic process

inferred from biological aspect of ancestor with PANTHER:PTN000188395

(GO Reference Genome Project, 2011-)

Cellular Component (4 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

located_in cytosol

inferred from direct assay

(Gradilla et al., 2011, Aguilera et al., 2000)

located_in nucleus

inferred from direct assay

(Gradilla et al., 2011)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

is_active_in cytoplasm

inferred from biological aspect of ancestor with PANTHER:PTN000188395

(GO Reference Genome Project, 2011-)

is_active_in nucleus

inferred from biological aspect of ancestor with PANTHER:PTN000188512

(GO Reference Genome Project, 2011-)

part_of ubiquitin ligase complex

inferred from biological aspect of ancestor with PANTHER:PTN000188395

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

RING-BETWEEN-RING UBIQUITIN LIGASES

Protein Family (UniProt)

Belongs to the RBR family. Ariadne subfamily. (Q94981)

Protein Signatures (InterPro)

Catalytic Activity (EC/Rhea)

ubiquitin protein ligase activity

Summaries

Gene Snapshot

Gene Group (FlyBase)

RING-BETWEEN-RING UBIQUITIN LIGASES -: E3 (ubiquitin) ligases interact with ubiquitin-E2 (ubiquitin-conjugating) enzymes and the target protein, transferring the ubiquitin from the E2 to the substrate. RING-between-RING (RBR) E3 ligases are characterised by three closely spaced domains: RING1 (a classical RING finger domain), a In-Betweeen-RING (IBR) domain and a RING2 domain. The mechanism of action is described as RING/HECT-like, with the RING1 domain facilitating E2-transfer (E3 RING-like) to form a thioester intermediate with a cysteine in RING2 which then modifies the substrate protein (HECT-like). (Adapted from PMID:24469331).

Protein Function (UniProtKB)

Atypical E3 ubiquitin-protein ligase, which catalyzes ubiquitination of target proteins together with ubiquitin-conjugating enzyme E2 Ubc10 (PubMed:10880484, PubMed:21900267, PubMed:29689197). Controls the subcellular localization and morphology of muscle nuclei (myonuclei) by regulating the protein levels and distribution of the LINC (LInker of Nucleoskeleton and Cytoskeleton) complex (PubMed:29689197). Functions by mediating the monoubiquitination of the LINC complex subunit koi leading to its subsequent proteasomal degradation (PubMed:29689197). Appears to function, at least partially redundantly, with the RBR E3 ligase family member park in nuclear localization and morphology (PubMed:29689197). Likely to function in metamorphosis by regulating the proteins levels of EcR isoform A (ECR-A) and its heterodimeric partner usp, via the ubiquitination and subsequent degradation of ECR-A (PubMed:21900267).

(UniProt, Q94981)

Summary (Interactive Fly)

E3 ubiquitin-ligase essential for neuronal development - mutants display a lower rate of spontaneous neurotransmitter release due to failures at the pre-synaptic side - evoked release in Ari-1 mutants was enhanced in a Ca(2+) dependent manner without modifications in the number of active zones, indicating that the probability of release per synapse is increased in these mutants - regulates myonuclear organization together with Parkin and is associated with aortic aneurysms

Interactive Fly

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\ari-1 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q94981)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.53

Gene model reviewed during 5.46

Low-frequency RNA-Seq exon junction(s) not annotated.

Annotated transcripts do not represent all supported alternative splices within 5' UTR.

Gene model reviewed during 5.45

Tissue-specific extension of 3' UTRs observed during later stages (FBrf0218523, FBrf0219848); all variants may not be annotated

Gene model reviewed during 5.56

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

ari-1-RA

FBtr0089506

NM_078675

2656

503

ari-1-RB

FBtr0089507

NM_167610

2635

503

ari-1-RC

FBtr0089508

NM_206777

2811

503

ari-1-RD

FBtr0306057

NM_001258807

4416

503

ari-1-RE

FBtr0332850

NM_001272742

3234

503

ari-1-RF

FBtr0332851

NM_001272743

3350

503

Additional Transcript Data and Comments

Reported size (kB)

4.3 (northern blot)

(Aguilera et al., 2000)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

ari-1-PA

FBpp0088499

58.9

503

5.01

FBpp0088500

58.9

503

5.01

FBpp0089023

58.9

503

5.01

FBpp0297199

58.9

503

5.01

FBpp0305073

58.9

503

5.01

FBpp0305074

58.9

503

5.01

Q94981

NP_001259672

AGB95514

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

503 aa isoforms: ari-1-PA, ari-1-PB, ari-1-PC, ari-1-PD, ari-1-PE, ari-1-PF

Additional Polypeptide Data and Comments

Reported size (kDa)

503 (aa); 59 (kD observed); 59 (kD predicted)

(Aguilera et al., 2000)

Comments

External Data

Subunit Structure (UniProtKB)

Can form homodimers (PubMed:10880484, PubMed:29689197). Interacts (via RING-type 1 zinc finger) with Ubc10 (PubMed:10880484, PubMed:29689197). Interacts with the LINC complex member koi (PubMed:29689197). Interacts with park (PubMed:29689197). Interacts with ari-2 (PubMed:29689197). Specifically interacts with isoform ECR-A of EcR (PubMed:21900267).

(UniProt, Q94981)

Post Translational Modification

Autophosphorylated.

(UniProt, Q94981)

Domain

Members of the RBR family are atypical E3 ligases. They interact with E2 conjugating enzymes such as Ubc10 and function like HECT-type E3 enzymes: they bind E2s via the first RING-type zinc finger, but require an obligate trans-thiolation step during the ubiquitin transfer, requiring a conserved active site Cys residue in the second RING-type zinc finger. The active site probably forms a thioester intermediate with ubiquitin taken from the active-site cysteine of the E2 before ultimately transferring it to a Lys residue on the substrate.

(UniProt, Q94981)

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\ari-1 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

ari-1_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-1.51

Transcript Expression

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage -- adult stage

(Aguilera et al., 2000)

larval stage

organism | ubiquitous

(Aguilera et al., 2000)

adult stage

adult central nervous system

(Aguilera et al., 2000)

adult stage | female

gonad

(Aguilera et al., 2000)

adult stage & oogenesis

nurse cell

(Aguilera et al., 2000)

follicle cell

(Aguilera et al., 2000)

Additional Descriptive Data

ari-1 transcripts are detected at all developmental stages, with levels increasing from embryogenesis onwards. The peak is reached at metamorphosis and maintained in adults. ari-1 is detected in all tissues, with high levels in the adult CNS and female gonads.

(Aguilera et al., 2000)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

western blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage -- adult stage

(Aguilera et al., 2000)

unfertilized egg stage

unfertilized egg

(Aguilera et al., 2000)

Additional Descriptive Data

Although ari-1 protein is detected mainly in the cytoplasm in Western blots of nuclear vs. cytoplasm enriched fractions, it does contain a putative nuclear localization signal.

(Aguilera et al., 2000)

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in cytosol

inferred from direct assay

(Gradilla et al., 2011, Aguilera et al., 2000)

located_in nucleus

inferred from direct assay

(Gradilla et al., 2011)

Expression Deduced from Reporters

Reporter: TI{2A-GAL4}ari-1^T-TG4.0

Stage

Tissue/Position (including subcellular localization)

Reference

third instar larval stage

larval muscle system

(Tan et al., 2018)

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\ari-1 in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\ari-1

View exonic expression by tissue for Dmel\ari-1

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

32796/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0017418

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0017418

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0017418

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG5659

Flygut - An atlas of the Drosophila adult midgut

FBgn0017418

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 19 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 7 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of ari-1

Transgenic constructs containing regulatory region of ari-1

Aberrations (Deficiencies and Duplications) ( 3 )

Inferred from experimentation ( 3 )

Gene duplicated in

Dp(1;3)JC153

(Aguilera et al., 2000, de la Pompa, 1994)

Dp(1;3)JC153-R4-III

(Aguilera et al., 2000)

Gene not duplicated in

Dp(1;3)JC153-R5

(Aguilera et al., 2000)

Inferred from location ( 11 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal

lethal | embryonic stage | germline clone | non-rescuable maternal effect | recessive

ari-1³⁷⁴⁷

lethal | pupal stage | recessive

ari-1³⁷⁴⁷

lethal - all die before end of prepupal stage, with Scer\GAL4^how-24B

ari-1^UAS.cFa

lethal - all die before end of prepupal stage, with Scer\GAL4^αTub84B.PL

lethal - all die before end of pupal stage | recessive

partially lethal (with ari-1¹¹⁹⁹)

ari-1³⁷⁴⁷

partially lethal (with ari-1³⁷⁴⁷)

ari-1¹¹⁹⁹

partially lethal | recessive

ari-1^T-GT15

partially lethal - majority die, with Scer\GAL4^phtm.PU

partially lethal - majority die, with Scer\GAL4^pnr-MD237

ari-1^GD11725

partially lethal - majority die (with Df(1)BSC352)

viable

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

ari-1^GD11725

viable, with Scer\GAL4^NP1063

ari-1^UAS.cFa

viable, with Scer\GAL4^tin.CΔ4

ari-1^GD11725

Sterility

Allele

female sterile (with Dp(1;3)JC153)

ari-1³⁷⁴⁷

fertile

ari-1^EY08909

Other Phenotypes

Allele

abnormal courtship behavior | male | recessive

ari-1^unspecified

abnormal jumping | recessive

ari-1^unspecified

abnormal locomotor behavior | adult stage | recessive

ari-1^T-GT15

abnormal locomotor behavior | recessive

ari-1^unspecified

abnormal neuroanatomy | adult stage | somatic clone

ari-1⁵⁷⁷⁷

abnormal neurophysiology | adult stage | somatic clone

abnormal neurophysiology | larval stage | recessive

abnormal size | recessive | third instar larval stage

abnormal size | somatic clone

abnormal size | third instar larval stage (with ari-1^A)

ari-1^D

abnormal size | third instar larval stage (with ari-1^B)

ari-1^D

abnormal size | third instar larval stage (with ari-1^D)

flightless | recessive

short lived, with Scer\GAL4^da.G32, ari-1^A

ari-1^UAS.cFa

short lived, with Scer\GAL4^da.G32, ari-1^UAS.cFa

ari-1^A

short lived (with ari-1^A)

ari-1^D

short lived (with ari-1^B)

ari-1^D

short lived (with ari-1^D)

short lived (with Df(1)BSC352)

short lived (with Dp(1;3)DC342)

ari-1^A

short lived | recessive

some die during prepupal stage | recessive, with Scer\GAL4^how-24B

ari-1^UAS.cFa

some die during prepupal stage | recessive, with Scer\GAL4^αTub84B.PL

some die during pupal stage, with Scer\GAL4^pnr-MD237

ari-1^GD11725

some die during pupal stage | recessive

visible, with Scer\GAL4^Bx-MS1096

ari-1^UAS.cFa

visible, with Scer\GAL4^Dll-md23

ari-1^UAS.cFa

visible, with Scer\GAL4^dpp.PS

ari-1^UAS.cFa

visible | recessive

ari-1^unspecified

Phenotype manifest in

Allele

adult brain

ari-1⁵⁷⁷⁷

adult cervical connective

ari-1⁵⁷⁷⁷

adult optic lobe

ari-1³⁷⁴⁷

adult ventral nerve cord

ari-1⁵⁷⁷⁷

coxal tergal remotor muscle cell (with ari-1¹¹⁹⁹)

ari-1³⁷⁴⁷

coxal tergal remotor muscle cell (with ari-1³⁷⁴⁷)

ari-1¹¹⁹⁹

embryonic/larval hypodermal muscle cell | third instar larval stage

embryonic/larval hypodermal muscle cell | third instar larval stage (with ari-1^A)

embryonic/larval hypodermal muscle cell | third instar larval stage (with ari-1^B)

embryonic/larval hypodermal muscle cell | third instar larval stage (with ari-1^D)

embryonic/larval hypodermal muscle cell | third instar larval stage (with Df(1)BSC352)

embryonic/larval neuromuscular junction | larval stage

eye | adult stage | somatic clone

germline cyst (with ari-1¹¹⁹⁹)

ari-1³⁷⁴⁷

germline cyst (with ari-1³⁷⁴⁷)

ari-1¹¹⁹⁹

germline cyst (with Dp(1;3)JC153)

ari-1³⁷⁴⁷

macrochaeta

mesothoracic tergotrochanter muscle cell (with ari-1¹¹⁹⁹)

ari-1³⁷⁴⁷

mesothoracic tergotrochanter muscle cell (with ari-1³⁷⁴⁷)

ari-1¹¹⁹⁹

microchaeta

microtubule | third instar larval stage

nuclear envelope | third instar larval stage

nuclear envelope | third instar larval stage (with ari-1^A)

ari-1^D

nuclear envelope | third instar larval stage (with ari-1^B)

ari-1^D

nuclear envelope | third instar larval stage (with ari-1^D)

nucleus | third instar larval stage

nucleus | third instar larval stage (with ari-1^A)

nucleus | third instar larval stage (with ari-1^B)

nucleus | third instar larval stage (with ari-1^D)

nucleus | third instar larval stage (with Df(1)BSC352)

ovariole (with ari-1¹¹⁹⁹)

ari-1³⁷⁴⁷

ovariole (with ari-1³⁷⁴⁷)

ari-1¹¹⁹⁹

ovariole (with Dp(1;3)JC153)

ari-1³⁷⁴⁷

photoreceptor cell R1 & rough endoplasmic reticulum | somatic clone

ari-1³⁷⁴⁷

photoreceptor cell R2 & rough endoplasmic reticulum | somatic clone

ari-1³⁷⁴⁷

photoreceptor cell R3 & rough endoplasmic reticulum | somatic clone

ari-1³⁷⁴⁷

photoreceptor cell R4 & rough endoplasmic reticulum | somatic clone

ari-1³⁷⁴⁷

photoreceptor cell R5 & rough endoplasmic reticulum | somatic clone

ari-1³⁷⁴⁷

photoreceptor cell R6 & rough endoplasmic reticulum | somatic clone

ari-1³⁷⁴⁷

photoreceptor cell R7 & rough endoplasmic reticulum | somatic clone

ari-1³⁷⁴⁷

rhabdomere R1 | somatic clone

ari-1³⁷⁴⁷

rhabdomere R2 | somatic clone

ari-1³⁷⁴⁷

rhabdomere R3 | somatic clone

ari-1³⁷⁴⁷

rhabdomere R4 | somatic clone

ari-1³⁷⁴⁷

rhabdomere R5 | somatic clone

ari-1³⁷⁴⁷

rhabdomere R6 | somatic clone

ari-1³⁷⁴⁷

wing, with Scer\GAL4^Bx-MS1096

ari-1^UAS.cFa

wing, with Scer\GAL4^Dll-md23

ari-1^UAS.cFa

wing, with Scer\GAL4^dpp.PS

ari-1^UAS.cFa

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (16)

Hsap\ARIH1

14 of 14

Yes

Hsap\ARIH2

5 of 14

Hsap\ANKIB1

3 of 14

Hsap\CUL9

3 of 14

Yes

Hsap\RBCK1

2 of 14

Yes

Hsap\RNF19A

2 of 14

Yes

Hsap\RNF19B

2 of 14

Yes

Hsap\RNF144A

2 of 14

Hsap\RNF144B

2 of 14

Hsap\RNF217

2 of 14

Yes

Hsap\CUL7

1 of 14

Yes

Hsap\PRKN

1 of 14

Hsap\RNF14

1 of 14

Yes

Hsap\RNF31

1 of 14

Hsap\RNF216

1 of 14

Hsap\SHARPIN

1 of 14

Yes

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (14)

Rnor\Arih1

14 of 14

Yes

Rnor\Arih2

5 of 14

Rnor\Ankib1

3 of 14

Rnor\Cul9

3 of 14

Yes

Rnor\Rnf19a

2 of 14

Yes

Rnor\Rnf19b

2 of 14

Yes

Rnor\Rnf144a

2 of 14

Rnor\Rnf144b

2 of 14

Rnor\Cul7

1 of 14

Yes

Rnor\Prkn

1 of 14

Rnor\Rnf14

1 of 14

Yes

Rnor\Rnf31

1 of 14

Rnor\Rnf217

1 of 14

Rnor\Sharpin

1 of 14

Yes

Mus musculus (laboratory mouse) (15)

Mmus\Arih1

14 of 14

Yes

Mmus\Arih2

5 of 14

Mmus\Cul9

3 of 14

Yes

Mmus\Ankib1

2 of 14

Mmus\Rnf19a

2 of 14

Yes

Mmus\Rnf19b

2 of 14

Yes

Mmus\Rnf144a

2 of 14

Mmus\Rnf144b

2 of 14

Mmus\Rnf217

2 of 14

Yes

Mmus\Cul7

1 of 14

Yes

Mmus\Prkn

1 of 14

Mmus\Rbck1

1 of 14

Yes

Mmus\Rnf14

1 of 14

Yes

Mmus\Rnf31

1 of 14

Mmus\Sharpin

1 of 14

Yes

Xenopus tropicalis (Western clawed frog) (13)

Xtro\arih1

11 of 13

Yes

3 of 13

1 of 13

1 of 13

1 of 13

Yes

Xtro\LOC108645237

1 of 13

Yes

Xtro\rbck1

1 of 13

Yes

Xtro\rnf14.2

1 of 13

Yes

Xtro\rnf14.5

1 of 13

Yes

Xtro\rnf19b

1 of 13

Yes

Xtro\rnf31

1 of 13

Xtro\rnf144a

1 of 13

Xtro\rnf144b

1 of 13

Danio rerio (Zebrafish) (25)

Drer\arih1

14 of 14

Yes

Drer\arih1l

14 of 14

Yes

5 of 14

2 of 14

2 of 14

2 of 14

Yes

Drer\rnf19b

2 of 14

Yes

Drer\rnf144ab

2 of 14

Drer\rnf144b

2 of 14

Drer\rnf217

2 of 14

Yes

Drer\shrprbck1r

2 of 14

Yes

Drer\si:ch211-278j3.3

2 of 14

Yes

Drer\cul9

1 of 14

Yes

Drer\LOC558876

1 of 14

Yes

Drer\LOC794117

1 of 14

Yes

Drer\LOC100334178

1 of 14

Drer\LOC108179742

1 of 14

Drer\LOC110437942

1 of 14

Yes

Drer\prkn

1 of 14

Drer\rbck1

1 of 14

Yes

Drer\rnf14

1 of 14

Yes

Drer\rnf144aa

1 of 14

Drer\rnf216

1 of 14

Drer\sharpin

1 of 14

Yes

Drer\si:dkey-181m9.8

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (14)

Cele\ari-1.1

13 of 14

Yes

Cele\ari-1.3

13 of 14

Yes

Cele\ari-1.2

12 of 14

Yes

Cele\ari-1.4

11 of 14

Yes

Cele\ari-2

5 of 14

Cele\C17H11.4

5 of 14

Yes

Cele\C17H11.6

1 of 14

Cele\F56D2.2

1 of 14

Cele\F56D2.5

1 of 14

Yes

Cele\pdr-1

1 of 14

Cele\T20F5.6

1 of 14

Yes

Cele\tag-314

1 of 14

Yes

Cele\Y49F6B.9

1 of 14

Cele\Y57A10A.31

1 of 14

Anopheles gambiae (African malaria mosquito) (7)

Agam\AgaP_AGAP010891

12 of 12

Yes

Agam\AgaP_AGAP005977

5 of 12

Agam\AgaP_AGAP004573

2 of 12

Yes

Agam\AgaP_AGAP005143

2 of 12

Agam\AgaP_AGAP009239

2 of 12

Yes

Agam\AgaP_AGAP006580

1 of 12

Agam\AgaP_AGAP007788

1 of 12

Arabidopsis thaliana (thale-cress) (45)

Atha\ARI1

12 of 13

Yes

Atha\ARI2

12 of 13

Yes

Atha\ARI3

11 of 13

Yes

Atha\ARI4

11 of 13

Yes

9 of 13

8 of 13

8 of 13

8 of 13

8 of 13

7 of 13

7 of 13

6 of 13

4 of 13

Yes

Atha\ARI14

4 of 13

Yes

Atha\ARI15

4 of 13

Yes

Atha\ARI16

4 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Atha\NHL8

1 of 13

Saccharomyces cerevisiae (Brewer's yeast) (2)

Scer\HEL1

12 of 13

Yes

Scer\ITT1

1 of 13

Schizosaccharomyces pombe (Fission yeast) (2)

Spom\SPAC328.02

12 of 12

Yes

Spom\itt1

1 of 12

Yes

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:ari-1. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (6)

10 of 13

9 of 13

4 of 13

3 of 13

1 of 13

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

muscle and aortic defects, ARIH1-related

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Allele

Disease

Interaction

References

ari-1³⁷⁴⁷

exacerbates Parkinson's disease

modeled by comt⁶

(Ramírez et al., 2021)

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

ARIH1; ariadne RBR E3 ubiquitin protein ligase 1

14 of 14

ARIADNE RBR E3 UBIQUITIN PROTEIN LIGASE 1; ARIH1

ARIH2; ariadne RBR E3 ubiquitin protein ligase 2

5 of 14

605615

ANKIB1; ankyrin repeat and IBR domain containing 1

3 of 14

620069

CUL9; cullin 9

3 of 14

607489

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Dmel gene

Ortholog showing functional complementation

Supporting References

ari-1

Hsap\ARIH1

(Tan et al., 2018)

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

ari-1

two hybrid, anti tag coimmunoprecipitation, anti tag western blot

(Tan et al., 2018, Aguilera et al., 2000)

ari-1 - EcR

two hybrid, anti tag coimmunoprecipitation, anti tag western blot, enzymatic study, western blot

(Gradilla et al., 2011)

ari-1 - Ubc10

anti tag coimmunoprecipitation, anti tag western blot, two hybrid

(Tan et al., 2018, Aguilera et al., 2000)

ari-1 - ari-2

anti tag coimmunoprecipitation, anti tag western blot

(Tan et al., 2018)

ari-1 - koi

anti tag coimmunoprecipitation, anti tag western blot, enzymatic study, western blot

(Tan et al., 2018)

ari-1 - park

anti tag coimmunoprecipitation, anti tag western blot

(Tan et al., 2018)

ari-1 - usp

two hybrid

(Gradilla et al., 2011)

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

ari-1

suppressible

bon

(Gradilla et al., 2011)

ari-1

suppressible

EcR

(Gradilla et al., 2011)

ari-1

suppressible

ftz-f1

(Gradilla et al., 2011)

suppressible

(Tan et al., 2018)

enhanceable

(Tan et al., 2018)

suppressible

(Gradilla et al., 2011)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

cindr

suppressible

ari-1

(Ketosugbo et al., 2017)

comt

enhanceable

ari-1

(Ramírez et al., 2021)

External Data

Subunit Structure (UniProtKB)

(UniProt, Q94981 )

Linkouts

BioGRID - A database of protein and genetic interactions.

59116

DroID - A comprehensive database of gene and protein interactions.

FBgn0017418

MIST (genetic) - An integrated Molecular Interaction Database

32796

MIST (protein-protein) - An integrated Molecular Interaction Database

32796

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Mitophagy - animal

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

1-59

Cytogenetic map

16F7-16F7

Sequence location

X:18,130,392..18,137,784 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

16F7-16F7

Limits computationally determined from genome sequence between P{EP}EP970 and P{EP}ari-1^EP317

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

1-59

(FlyBase, 2016)

1-59.1

(Comeron et al., 2012)

1-57.6--58.7

(Barbas, 1996.6.5)

1-57.6-58.7

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (35)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

49069

w¹¹¹⁸; P{GMR24B09-GAL4}attP2

49105

w¹¹¹⁸; P{GMR25A06-GAL4}attP2

52671

w¹¹¹⁸; P{GMR23H11-lexA}attP40

68667

w¹¹¹⁸; P{R25A06-p65.AD}attP40

70135

w¹¹¹⁸; P{R23H11-p65.AD}attP40

10142

w¹¹¹⁸ P{EP}ari-1^EP317

Korea

12284

P{VT063626-GAL4}attP2

16029

P{GMR23H11-lexA}attP2

10675

P{EP}ari-1^G523

16686

P{VT063626-lexA::GADfl}attP40

VDRC

v35029

w¹¹¹⁸ P{GD11725}v35029

v39591

w¹¹¹⁸ P{GD11725}v39591

More stocks available...

Genomic Clones (13)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (124)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

RE69116

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0017418

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0017418

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Gradilla et al., 2011, Aguilera et al., 2000)

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

ari-1 specifically binds the A isoform of EcR and ubiquitylates it.

(Gradilla et al., 2011)

ari-1 is required for the correct differentiation of most cell types in the adult organism.

(Aguilera et al., 2000)

Relationship to Other Genes

Source for database merge of

Source for merge of: ari l(1)16Fj

(Ferrus, 1999.3.4)

Additional comments

Nomenclature History

Source for database identify of

Source for identity of: ari CG5659

(Li, 1999.11)

Nomenclature comments

Etymology

The name "ariadne" refers to the loss of clues to find the correct path of axonal projection, and it evokes the Greek myth of the thread that Ariadne gave to Theseus to find his way in the Minotaur's labyrinth.

(Aguilera et al., 2000)

Synonyms and Secondary IDs (13)

Reported As

Symbol Synonym

AAS65398.1

(Eisenhaber et al., 2007)

ARI1_DROME

(Storm and Sonnhammer, 2003)

Ari-1

(Azevedo and Baylies, 2020, Oriel and Lasko, 2018, Ying et al., 2011, Gradilla and Ferrus, 2004)

CG5659

(Lin et al., 2015, Haelterman et al., 2014, Ying et al., 2011, Perkins et al., 2009, Misra, 2001.8.16, Mount, 2001.8.14)

HL-V

(Ferrus, 1999.3.4, Prado et al., 1999)

ari

(Moynihan et al., 1999, Cowley, 1998.9.24, de la Pompa, 1994, Ferrus et al., 1990)

ari-1

(Bellen, 2024.9.12, Peng et al., 2024, Ecovoiu et al., 2022, Baek et al., 2021, Meltzer et al., 2019, Story et al., 2019, Baranski et al., 2018, Tan et al., 2018, Ketosugbo et al., 2017, Eliazer et al., 2014, Gonzalez et al., 2014, Haelterman et al., 2014, McPhee et al., 2013, Vaque et al., 2013, Du et al., 2011, Langille and Clark, 2007, Kuo et al., 2006, Martin et al., 2006, Gleason, 2005)

ari-1a

(Gradilla et al., 2011)

l(1)16Fj

Name Synonyms

Ariadne

(Felix et al., 2012)

Ariadne-1

(Oriel and Lasko, 2018, Gradilla and Ferrus, 2004)

ariadne

(Dason et al., 2009, Gleason, 2005, Barbas, 1996.6.5, Barbas, 1996.6.5)

ariadne 1

Secondary FlyBase IDs

FBgn0014203

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 67 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

32796

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q94981

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

Q94981

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q94981

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

32796/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0017418

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0017418

FlyMine - An integrated database for Drosophila genomics

FBgn0017418

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG5659

MARRVEL_MODEL - MARRVEL (model organism gene)

32796

Linkouts

BioGRID - A database of protein and genetic interactions.

59116

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0017418

DroID - A comprehensive database of gene and protein interactions.

FBgn0017418

DRSC - Results frm RNAi screens

FBgn0017418

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

ari-1_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0017418

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0017418

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG5659

Flygut - An atlas of the Drosophila adult midgut

FBgn0017418

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0017418

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Mitophagy - animal

MIST (genetic) - An integrated Molecular Interaction Database

32796

MIST (protein-protein) - An integrated Molecular Interaction Database

32796

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

References (72)

The following external sites may use different assemblies or annotations than FlyBase.

-1.51

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