FlyBase Gene Report: Dmel\ari-1

General Information

Symbol

Dmel\ari-1

Species

D. melanogaster

Name

ariadne 1

Annotation Symbol

CG5659

Feature Type

protein_coding_gene

FlyBase ID

FBgn0017418

Gene Model Status

Current

Stock Availability

11 publicly available

Gene Snapshot

ariadne 1 (ari-1) encodes an E3 ubiquitin ligase that interacts with the E2 ubiquitin conjugating enzyme encoded by Ubc10. The product of ari-1 and Ubc10 jointly ubiquitylate isoform A of the ecdysone receptor encoded by EcR. [Date last reviewed: 2019-03-07]

Other Summaries

Alliance
Auto summary
Gene Group
UniProtKB
Interactive Fly

Also Known As

ari, ariadne, HL-V

Key Links

Genomic Location

Cytogenetic map

16F7-16F7

Sequence location

X:18,130,392..18,137,784 [-]

Recombination map

1-59

RefSeq locus

NC_004354 REGION:18130392..18137784

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse GBrowse

Help me choose

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

Gene Ontology (GO) Annotations (17 terms)

Molecular Function (4 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

enables ubiquitin conjugating enzyme binding

inferred from physical interaction with FLYBASE:Ubc10; FB:FBgn0026316

(Tan et al., 2018, Aguilera et al., 2000)

enables ubiquitin protein ligase activity

inferred from direct assay

(Gradilla et al., 2011)

inferred from mutant phenotype

(Tan et al., 2018)

Terms Based on Predictions or Assertions (4 terms)

CV Term

Evidence

References

enables metal ion binding

inferred from electronic annotation with InterPro:IPR018957

(assigned by InterPro )

(FlyBase Curators et al., 2004-)

enables ubiquitin conjugating enzyme binding

inferred from biological aspect of ancestor with PANTHER:PTN000188395

(assigned by GO_Central )

(Gaudet et al., 2011)

enables ubiquitin protein ligase activity

inferred from biological aspect of ancestor with PANTHER:PTN000188395

(assigned by GO_Central )

(Gaudet et al., 2011)

enables zinc ion binding

inferred from sequence model

(Ying et al., 2011)

Biological Process (9 terms)

Terms Based on Experimental Evidence (7 terms)

CV Term

Evidence

References

involved_in endoplasmic reticulum organization

inferred from mutant phenotype

(Aguilera et al., 2000)

involved_in oogenesis

inferred from mutant phenotype

(Aguilera et al., 2000)

involved_in positive regulation of neurogenesis

inferred from mutant phenotype

(Aguilera et al., 2000)

involved_in positive regulation of proteasomal protein catabolic process

inferred from mutant phenotype

(Tan et al., 2018)

involved_in protein monoubiquitination

inferred from direct assay

(Tan et al., 2018)

involved_in protein ubiquitination

inferred from direct assay

(Gradilla et al., 2011)

involved_in ubiquitin-dependent protein catabolic process

inferred from mutant phenotype

(Gradilla et al., 2011)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

involved_in positive regulation of proteasomal ubiquitin-dependent protein catabolic process

inferred from biological aspect of ancestor with PANTHER:PTN000188395

(assigned by GO_Central )

(Gaudet et al., 2011)

involved_in protein polyubiquitination

inferred from biological aspect of ancestor with PANTHER:PTN000188395

(assigned by GO_Central )

(Gaudet et al., 2011)

involved_in ubiquitin-dependent protein catabolic process

inferred from biological aspect of ancestor with PANTHER:PTN000188395

(assigned by GO_Central )

(Gaudet et al., 2011)

Cellular Component (4 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

located_in cytosol

inferred from direct assay

(Gradilla et al., 2011, Aguilera et al., 2000)

located_in nucleus

inferred from direct assay

(Gradilla et al., 2011)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

located_in cytoplasm

inferred from biological aspect of ancestor with PANTHER:PTN000188395

(assigned by GO_Central )

(Gaudet et al., 2011)

located_in nucleus

inferred from biological aspect of ancestor with PANTHER:PTN000188512

(assigned by GO_Central )

(Gaudet et al., 2011)

part_of ubiquitin ligase complex

inferred from biological aspect of ancestor with PANTHER:PTN000188395

(assigned by GO_Central )

(Gaudet et al., 2011)

Gene Group (FlyBase)

RING-BETWEEN-RING UBIQUITIN LIGASES

Protein Family (UniProt)

Belongs to the RBR family. Ariadne subfamily. (Q94981)

Protein Signatures (InterPro)

Summaries

Gene Group (FlyBase)

RING-BETWEEN-RING UBIQUITIN LIGASES -: E3 (ubiquitin) ligases interact with ubiquitin-E2 (ubiquitin-conjugating) enzymes and the target protein, transferring the ubiquitin from the E2 to the substrate. RING-between-RING (RBR) E3 ligases are characterised by three closely spaced domains: RING1 (a classical RING finger domain), a In-Betweeen-RING (IBR) domain and a RING2 domain. The mechanism of action is described as RING/HECT-like, with the RING1 domain facilitating E2-transfer (E3 RING-like) to form a thioester intermediate with a cysteine in RING2 which then modifies the substrate protein (HECT-like). (Adapted from PMID:24469331).

Protein Function (UniProtKB)

Atypical E3 ubiquitin-protein ligase, which catalyzes ubiquitination of target proteins together with ubiquitin-conjugating enzyme E2 Ubc10 (PubMed:10880484, PubMed:21900267, PubMed:29689197). Controls the subcellular localization and morphology of muscle nuclei (myonuclei) by regulating the protein levels and distribution of the LINC (LInker of Nucleoskeleton and Cytoskeleton) complex (PubMed:29689197). Functions by mediating the monoubiquitination of the LINC complex subunit koi leading to its subsequent proteasomal degradation (PubMed:29689197). Appears to function, at least partially redundantly, with the RBR E3 ligase family member park in nuclear localization and morphology (PubMed:29689197). Likely to function in metamorphosis by regulating the proteins levels of EcR isoform A (ECR-A) and its heterodimeric partner usp, via the ubiquitination and subsequent degradation of ECR-A (PubMed:21900267).

(UniProt, Q94981)

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\ari-1 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Comments on Gene Model

Gene model reviewed during 5.53

Gene model reviewed during 5.46

Low-frequency RNA-Seq exon junction(s) not annotated.

Annotated transcripts do not represent all supported alternative splices within 5' UTR.

Gene model reviewed during 5.45

Tissue-specific extension of 3' UTRs observed during later stages (FBrf0218523, FBrf0219848); all variants may not be annotated

Gene model reviewed during 5.56

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

ari-1-RA

FBtr0089506

NM_078675

2656

503

ari-1-RB

FBtr0089507

NM_167610

2635

503

ari-1-RC

FBtr0089508

NM_206777

2811

503

ari-1-RD

FBtr0306057

NM_001258807

4416

503

ari-1-RE

FBtr0332850

NM_001272742

3234

503

ari-1-RF

FBtr0332851

NM_001272743

3350

503

Additional Transcript Data and Comments

Reported size (kB)

4.3 (northern blot)

(Aguilera et al., 2000)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

ari-1-PA

FBpp0088499

58.9

503

5.01

NP_523399

AAN09462

ari-1-PB

FBpp0088500

58.9

503

5.01

NP_728145

AAF48807

ari-1-PC

FBpp0089023

58.9

503

5.01

NP_996500

AAS65398

ari-1-PD

FBpp0297199

58.9

503

5.01

NP_001245736

AFH07449

ari-1-PE

FBpp0305073

58.9

503

5.01

NP_001259671

AGB95513

ari-1-PF

FBpp0305074

58.9

503

5.01

NP_001259672

AGB95514

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

503 aa isoforms: ari-1-PA, ari-1-PB, ari-1-PC, ari-1-PD, ari-1-PE, ari-1-PF

Additional Polypeptide Data and Comments

Reported size (kDa)

503 (aa); 59 (kD observed); 59 (kD predicted)

(Aguilera et al., 2000)

Comments

External Data

Subunit Structure (UniProtKB)

Can form homodimers (PubMed:10880484, PubMed:29689197). Interacts (via RING-type 1 zinc finger) with Ubc10 (PubMed:10880484, PubMed:29689197). Interacts with the LINC complex member koi (PubMed:29689197). Interacts with park (PubMed:29689197). Interacts with ari-2 (PubMed:29689197). Specifically interacts with isoform ECR-A of EcR (PubMed:21900267).

(UniProt, Q94981)

Post Translational Modification

Autophosphorylated.

(UniProt, Q94981)

Domain

Members of the RBR family are atypical E3 ligases. They interact with E2 conjugating enzymes such as Ubc10 and function like HECT-type E3 enzymes: they bind E2s via the first RING-type zinc finger, but require an obligate trans-thiolation step during the ubiquitin transfer, requiring a conserved active site Cys residue in the second RING-type zinc finger. The active site probably forms a thioester intermediate with ubiquitin taken from the active-site cysteine of the E2 before ultimately transferring it to a Lys residue on the substrate.

(UniProt, Q94981)

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\ari-1 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

ari-1_1

Linkouts

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage & oogenesis

nurse cell

(Aguilera et al., 2000)

follicle cell

(Aguilera et al., 2000)

larval stage

organism | ubiquitous

(Aguilera et al., 2000)

adult stage

adult central nervous system

(Aguilera et al., 2000)

adult stage | female

gonad

(Aguilera et al., 2000)

embryonic stage -- adult stage

(Aguilera et al., 2000)

Additional Descriptive Data

ari-1 transcripts are detected at all developmental stages, with levels increasing from embryogenesis onwards. The peak is reached at metamorphosis and maintained in adults. ari-1 is detected in all tissues, with high levels in the adult CNS and female gonads.

(Aguilera et al., 2000)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

western blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage -- adult stage

(Aguilera et al., 2000)

unfertilized egg stage

unfertilized egg

(Aguilera et al., 2000)

Additional Descriptive Data

Although ari-1 protein is detected mainly in the cytoplasm in Western blots of nuclear vs. cytoplasm enriched fractions, it does contain a putative nuclear localization signal.

(Aguilera et al., 2000)

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in cytosol

inferred from direct assay

(Gradilla et al., 2011, Aguilera et al., 2000)

located_in nucleus

inferred from direct assay

(Gradilla et al., 2011)

Expression Deduced from Reporters

Reporter: TI{2A-GAL4}ari-1^T-TG4.0

Stage

Tissue/Position (including subcellular localization)

Reference

third instar larval stage

embryonic/larval muscle system

(Tan et al., 2018)

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\ari-1 in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\ari-1

View exonic expression by tissue for Dmel\ari-1

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0017418

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG5659-RA

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG5659

Flygut - An atlas of the Drosophila adult midgut

FBgn0017418

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, and Transgenic Constructs

Classical and Insertion Alleles ( 18 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 5 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of ari-1

Transgenic constructs containing regulatory region of ari-1

Deletions and Duplications ( 3 )

Duplicated in

Dp(1;3)JC153

(Aguilera et al., 2000, de la Pompa, 1994)

Dp(1;3)JC153-R4-III

(Aguilera et al., 2000)

Not duplicated in

Dp(1;3)JC153-R5

(Aguilera et al., 2000)

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal

lethal | embryonic stage | germline clone | non-rescuable maternal effect | recessive

ari-1³⁷⁴⁷

lethal | pupal stage | recessive

ari-1³⁷⁴⁷

lethal - all die before end of prepupal stage, with Scer\GAL4^how-24B

ari-1^UAS.cFa

lethal - all die before end of prepupal stage, with Scer\GAL4^αTub84B.PL

lethal - all die before end of pupal stage | recessive

partially lethal (with ari-1¹¹⁹⁹)

ari-1³⁷⁴⁷

partially lethal (with ari-1³⁷⁴⁷)

ari-1¹¹⁹⁹

partially lethal | recessive

ari-1^T-GT15

partially lethal - majority die, with Scer\GAL4^phm.PU

partially lethal - majority die, with Scer\GAL4^pnr-MD237

ari-1^GD11725

partially lethal - majority die (with Df(1)BSC352)

viable

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

ari-1^GD11725

viable, with Scer\GAL4^NP1063

ari-1^UAS.cFa

viable, with Scer\GAL4^tin.CΔ4

ari-1^GD11725

Sterility

Allele

female sterile (with Dp(1;3)JC153)

ari-1³⁷⁴⁷

fertile

ari-1^EY08909

Other Phenotypes

Allele

courtship behavior defective | male | recessive

ari-1^unspecified

flightless | recessive

jumping defective | recessive

ari-1^unspecified

locomotor behavior defective | adult stage | recessive

ari-1^T-GT15

locomotor behavior defective | recessive

ari-1^unspecified

neuroanatomy defective | adult stage | somatic clone

ari-1⁵⁷⁷⁷

neurophysiology defective | adult stage | somatic clone

short lived, with Scer\GAL4^da.G32, ari-1^A

ari-1^UAS.cFa

short lived, with Scer\GAL4^da.G32, ari-1^UAS.cFa

ari-1^A

short lived (with ari-1^A)

ari-1^D

short lived (with ari-1^B)

ari-1^D

short lived (with ari-1^D)

short lived (with Df(1)BSC352)

short lived (with Dp(1;3)DC342)

ari-1^A

short lived | recessive

size defective | recessive | third instar larval stage

size defective | somatic clone

size defective | third instar larval stage (with ari-1^A)

ari-1^D

size defective | third instar larval stage (with ari-1^B)

ari-1^D

size defective | third instar larval stage (with ari-1^D)

some die during prepupal stage | recessive, with Scer\GAL4^how-24B

ari-1^UAS.cFa

some die during prepupal stage | recessive, with Scer\GAL4^αTub84B.PL

some die during pupal stage, with Scer\GAL4^pnr-MD237

ari-1^GD11725

some die during pupal stage | recessive

visible, with Scer\GAL4^Bx-MS1096

ari-1^UAS.cFa

visible, with Scer\GAL4^Dll-md23

ari-1^UAS.cFa

visible, with Scer\GAL4^dpp.PS

ari-1^UAS.cFa

visible | recessive

ari-1^unspecified

Phenotype manifest in

Allele

adult brain

ari-1⁵⁷⁷⁷

adult cervical connective

ari-1⁵⁷⁷⁷

adult optic lobe

ari-1³⁷⁴⁷

adult ventral nervous system

ari-1⁵⁷⁷⁷

coxal tergal remotor muscle (with ari-1¹¹⁹⁹)

ari-1³⁷⁴⁷

coxal tergal remotor muscle (with ari-1³⁷⁴⁷)

ari-1¹¹⁹⁹

embryonic/larval hypodermal muscle | third instar larval stage

embryonic/larval hypodermal muscle | third instar larval stage (with ari-1^A)

embryonic/larval hypodermal muscle | third instar larval stage (with ari-1^B)

embryonic/larval hypodermal muscle | third instar larval stage (with ari-1^D)

embryonic/larval hypodermal muscle | third instar larval stage (with Df(1)BSC352)

eye | adult stage | somatic clone

germline cyst (with ari-1¹¹⁹⁹)

ari-1³⁷⁴⁷

germline cyst (with ari-1³⁷⁴⁷)

ari-1¹¹⁹⁹

germline cyst (with Dp(1;3)JC153)

ari-1³⁷⁴⁷

macrochaeta

mesothoracic extracoxal depressor muscle 66 (with ari-1¹¹⁹⁹)

ari-1³⁷⁴⁷

mesothoracic extracoxal depressor muscle 66 (with ari-1³⁷⁴⁷)

ari-1¹¹⁹⁹

microchaeta

microtubule | third instar larval stage

nuclear envelope | third instar larval stage

nuclear envelope | third instar larval stage (with ari-1^A)

ari-1^D

nuclear envelope | third instar larval stage (with ari-1^B)

ari-1^D

nuclear envelope | third instar larval stage (with ari-1^D)

nucleus | third instar larval stage

nucleus | third instar larval stage (with ari-1^A)

nucleus | third instar larval stage (with ari-1^B)

nucleus | third instar larval stage (with ari-1^D)

nucleus | third instar larval stage (with Df(1)BSC352)

ovariole (with ari-1¹¹⁹⁹)

ari-1³⁷⁴⁷

ovariole (with ari-1³⁷⁴⁷)

ari-1¹¹⁹⁹

ovariole (with Dp(1;3)JC153)

ari-1³⁷⁴⁷

photoreceptor cell R1 & rough endoplasmic reticulum | somatic clone

ari-1³⁷⁴⁷

photoreceptor cell R2 & rough endoplasmic reticulum | somatic clone

ari-1³⁷⁴⁷

photoreceptor cell R3 & rough endoplasmic reticulum | somatic clone

ari-1³⁷⁴⁷

photoreceptor cell R4 & rough endoplasmic reticulum | somatic clone

ari-1³⁷⁴⁷

photoreceptor cell R5 & rough endoplasmic reticulum | somatic clone

ari-1³⁷⁴⁷

photoreceptor cell R6 & rough endoplasmic reticulum | somatic clone

ari-1³⁷⁴⁷

photoreceptor cell R7 & rough endoplasmic reticulum | somatic clone

ari-1³⁷⁴⁷

rhabdomere R1 | somatic clone

ari-1³⁷⁴⁷

rhabdomere R2 | somatic clone

ari-1³⁷⁴⁷

rhabdomere R3 | somatic clone

ari-1³⁷⁴⁷

rhabdomere R4 | somatic clone

ari-1³⁷⁴⁷

rhabdomere R5 | somatic clone

ari-1³⁷⁴⁷

rhabdomere R6 | somatic clone

ari-1³⁷⁴⁷

wing, with Scer\GAL4^Bx-MS1096

ari-1^UAS.cFa

wing, with Scer\GAL4^Dll-md23

ari-1^UAS.cFa

wing, with Scer\GAL4^dpp.PS

ari-1^UAS.cFa

Orthologs

Downloads

Download All DIOPT Orthologs

Download All OrthoDB Orthologs

Human Orthologs (via DIOPT v8.0)

Homo sapiens (Human) (13)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Hsap\ARIH1

14 of 15

Yes

Hsap\ARIH2

3 of 15

Hsap\ANKIB1

2 of 15

Hsap\CUL9

2 of 15

Yes

Hsap\MIR630

2 of 15

Yes

Hsap\CUL7

1 of 15

Yes

Hsap\RBCK1

1 of 15

Yes

Hsap\RNF19A

1 of 15

Yes

Hsap\RNF19B

1 of 15

Yes

Hsap\RNF144A

1 of 15

Hsap\RNF144B

1 of 15

Hsap\RNF217

1 of 15

Yes

Hsap\SHARPIN

1 of 15

Yes

Model Organism Orthologs (via DIOPT v8.0)

Mus musculus (laboratory mouse) (12)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Mmus\Arih1

15 of 15

Yes

Mmus\Arih2

3 of 15

Mmus\Cul9

2 of 15

Yes

Mmus\Ankib1

1 of 15

Mmus\Cul7

1 of 15

Yes

Mmus\Rbck1

1 of 15

Yes

Mmus\Rnf19a

1 of 15

Yes

Mmus\Rnf19b

1 of 15

Yes

Mmus\Rnf144a

1 of 15

Mmus\Rnf144b

1 of 15

Mmus\Rnf217

1 of 15

Yes

Mmus\Sharpin

1 of 15

Yes

Rattus norvegicus (Norway rat) (10)

Rnor\Arih1

13 of 13

Yes

Rnor\Arih2

3 of 13

Rnor\Ankib1

2 of 13

Yes

Rnor\Cul9

2 of 13

Yes

Rnor\LOC680835

1 of 13

Yes

Rnor\Rnf19a

1 of 13

Yes

Rnor\Rnf19b

1 of 13

Yes

Rnor\Rnf144a

1 of 13

Rnor\Rnf144b

1 of 13

Rnor\Sharpin

1 of 13

Yes

Xenopus tropicalis (Western clawed frog) (8)

Xtro\arih1

9 of 12

Yes

Xtro\arih2

2 of 12

Xtro\cul9

2 of 12

Yes

Xtro\ankib1

1 of 12

Yes

Xtro\rnf19a

1 of 12

Yes

Xtro\rnf144a

1 of 12

Xtro\rnf144b

1 of 12

Xtro\rnf217

1 of 12

Yes

Danio rerio (Zebrafish) (16)

Drer\arih1

14 of 15

Yes

Drer\arih1l

14 of 15

Yes

Drer\ankib1a

3 of 15

Yes

Drer\arih2

3 of 15

Drer\ankib1b

2 of 15

Yes

Drer\cul9

1 of 15

Yes

Drer\rbck1

1 of 15

Yes

Drer\rnf19a

1 of 15

Yes

Drer\rnf19b

1 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

Yes

Drer\sharpin

1 of 15

Yes

Drer\shrprbck1r

1 of 15

Yes

Drer\si:ch211-278j3.3

1 of 15

Yes

Caenorhabditis elegans (Nematode, roundworm) (12)

Cele\ari-1.4

12 of 15

Yes

Cele\ari-1.1

11 of 15

Yes

Cele\ari-1.2

11 of 15

Yes

Cele\ari-1.3

11 of 15

Yes

Cele\ari-2

3 of 15

Cele\C17H11.4

3 of 15

Yes

Cele\C17H11.6

1 of 15

Yes

Cele\F56D2.2

1 of 15

Yes

Cele\F56D2.5

1 of 15

Yes

Cele\pdr-1

1 of 15

Cele\tag-314

1 of 15

Yes

Cele\Y49F6B.9

1 of 15

Yes

Arabidopsis thaliana (thale-cress) (15)

Atha\ARI1

8 of 9

Yes

Atha\ARI2

8 of 9

Yes

Atha\ARI3

6 of 9

Yes

Atha\ARI4

6 of 9

Yes

5 of 9

5 of 9

5 of 9

5 of 9

4 of 9

4 of 9

3 of 9

2 of 9

Yes

Atha\ARI14

2 of 9

Yes

Atha\ARI15

2 of 9

Yes

Atha\ARI16

2 of 9

Yes

Saccharomyces cerevisiae (Brewer's yeast) (1)

Scer\HEL1

13 of 15

Yes

Schizosaccharomyces pombe (Fission yeast) (1)

Spom\SPAC328.02

11 of 12

Yes

Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG091905O6 )

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila suzukii

Spotted wing Drosophila

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA22657

Drosophila persimilis

Dper\GL26892

Drosophila willistoni

Dwil\GK16299

Drosophila virilis

Dvir\GJ16609

Drosophila mojavensis

Dmoj\GI15200

Drosophila grimshawi

Dgri\GH24087

Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091503DO )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Musca domestica

House fly

Mdoa\17009715

Glossina morsitans

Tsetse fly

Gmor\GMOY007991

Glossina morsitans

Tsetse fly

Gmor\GMOY001743

Lucilia cuprina

Australian sheep blowfly

Lcup\FF38_08363

Mayetiola destructor

Hessian fly

Mdes\Mdes010351

Mayetiola destructor

Hessian fly

Mdes\Mdes016048

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL013028

Anopheles darlingi

American malaria mosquito

Adar\ADAC000947

Anopheles gambiae

Malaria mosquito

Agam\AGAP010891

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ005416

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ016079

Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W017I )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Bombyx mori

Silkmoth

Bmor\BGIBMGA001417

Danaus plexippus

Monarch butterfly

Dple\DPOGS204817-PA

Heliconius melpomene

Postman butterfly

Hmel\HMEL007427

Apis florea

Little honeybee

Aflo\2238932676

Apis mellifera

Western honey bee

Amel\LOC413468

Bombus impatiens

Common eastern bumble bee

Bimp\BIMP20631

Bombus terrestris

Buff-tailed bumblebee

Bter\15210549

Linepithema humile

Argentine ant

Lhum\LH16658

Megachile rotundata

Alfalfa leafcutting bee

Mrot\MROTU:00058f

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG002045

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG002044

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG006648

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG007639

Dendroctonus ponderosae

Mountain pine beetle

Dpod\YQE_03269

Dendroctonus ponderosae

Mountain pine beetle

Dpod\YQE_09958

Tribolium castaneum

Red flour beetle

Tcas\TC004596

Tribolium castaneum

Red flour beetle

Tcas\TC002351

Pediculus humanus

Human body louse

Phum\PHUM400430

Rhodnius prolixus

Kissing bug

Rpro\RPRC014598

Cimex lectularius

Bed bug

Clec\CLEC012536

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI004803

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_04865

Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X015R )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strigamia maritima

European centipede

Smar\SMAR012328

Ixodes scapularis

Black-legged tick

Isca\ISCW001725

Stegodyphus mimosarum

African social velvet spider

Smim\KFM82463.1

Stegodyphus mimosarum

African social velvet spider

Smim\KFM56590.1

Tetranychus urticae

Two-spotted spider mite

Turt\tetur05g08730

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_307315

Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G01SC )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Arih1

Ciona intestinalis

Vase tunicate

Cint\ENSCING00000000894

Gallus gallus

Domestic chicken

Ggal\ARIH1

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v8.0)

Drosophila melanogaster (Fruit fly) (4)

Gene Symbol

Score

Source

Alignment

7 of 10

6 of 10

3 of 10

2 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

muscle and aortic defects, ARIH1-related

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

ARIH1; ariadne RBR E3 ubiquitin protein ligase 1

14 of 15

605624

ARIH2; ariadne RBR E3 ubiquitin protein ligase 2

3 of 15

605615

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Dmel gene

Ortholog showing functional complementation

Supporting References

ari-1

Hsap\ARIH1

(Tan et al., 2018)

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

View in Interactions Browser

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

ari-1

anti tag coimmunoprecipitation, anti tag western blot, two hybrid

(Tan et al., 2018, Aguilera et al., 2000)

ari-1 - EcR

anti tag coimmunoprecipitation, western blot, two hybrid, enzymatic study, anti tag western blot

(Gradilla et al., 2011)

ari-1 - Ubc10

two hybrid, anti tag coimmunoprecipitation, anti tag western blot

(Tan et al., 2018, Aguilera et al., 2000)

ari-1 - ari-2

anti tag coimmunoprecipitation, anti tag western blot

(Tan et al., 2018)

ari-1 - koi

enzymatic study, western blot, anti tag coimmunoprecipitation, anti tag western blot

(Tan et al., 2018)

ari-1 - park

anti tag coimmunoprecipitation, anti tag western blot

(Tan et al., 2018)

ari-1 - usp

two hybrid

(Gradilla et al., 2011)

Summary of Genetic Interactions

esyN Network Diagram

esyN Network Key:

Suppression

Enhancement

View in Interactions Browser

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

ari-1

suppressible

bon

(Gradilla et al., 2011)

ari-1

suppressible

EcR

(Gradilla et al., 2011)

ari-1

suppressible

ftz-f1

(Gradilla et al., 2011)

suppressible

(Tan et al., 2018)

enhanceable

(Tan et al., 2018)

suppressible

(Gradilla et al., 2011)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Subunit Structure (UniProtKB)

(UniProt, Q94981 )

Linkouts

BioGRID - A database of protein and genetic interactions.

59116

DroID - A comprehensive database of gene and protein interactions.

FBgn0017418

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

32796

MIST (genetic) - An integrated Molecular Interaction Database

ari-1

MIST (protein-protein) - An integrated Molecular Interaction Database

ari-1

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

External Data

Linkouts

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

ISG15 antiviral mechanism

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

1-59

Cytogenetic map

16F7-16F7

Sequence location

X:18,130,392..18,137,784 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

16F7-16F7

Limits computationally determined from genome sequence between P{EP}EP970 and P{EP}ari-1^EP317

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

1-59

(FlyBase, 2016)

1-59.1

(Comeron et al., 2012)

1-57.6--58.7

(Barbas, 1996.6.5)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (11)

Bloomington

10142

w¹¹¹⁸ P{EP}ari-1^EP317

29416

y¹ v¹; P{TRiP.JF03352}attP2

80125

y¹ w^*; PBac{UAS-ari-1.V5}VK00037

17507

y¹ w^67c23 P{EPgy2}ari-1^EY08909

80121

y¹ w^* TI{2A-GAL4}ari-1^T-TG4.0/FM7c

80122

y¹ w^* ari-1^A P{neoFRT}19A/FM7c, P{GAL4-Kr.C}DC1, P{UAS-GFP.S65T}DC5, sn⁺

80123

y¹ w^* ari-1^B P{neoFRT}19A/FM7c, P{GAL4-Kr.C}DC1, P{UAS-GFP.S65T}DC5, sn⁺

Kyoto

124486

w¹¹¹⁸ P{RS3}ari-1^UM-8334-3

103873

y^* w^* P{GawB}ari-1^NP1063 / FM7c

VDRC

v35029

w¹¹¹⁸ P{GD11725}v35029

v39591

w¹¹¹⁸ P{GD11725}v39591

Genomic Clones (13)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (135)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

RE69116

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0017418

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0017418

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

32796

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Gradilla et al., 2011, Aguilera et al., 2000)

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for identity of: ari CG5659

(Li, 1999.11)

Source for database merge of

Source for merge of: ari l(1)16Fj

(Ferrus, 1999.3.4)

Additional comments

Other Comments

ari-1 specifically binds the A isoform of EcR and ubiquitylates it.

(Gradilla et al., 2011)

ari-1 is required for the correct differentiation of most cell types in the adult organism.

(Aguilera et al., 2000)

Origin and Etymology

Discoverer

Etymology

The name "ariadne" refers to the loss of clues to find the correct path of axonal projection, and it evokes the Greek myth of the thread that Ariadne gave to Theseus to find his way in the Minotaur's labyrinth.

(Aguilera et al., 2000)

Identification

External Crossreferences and Linkouts ( 59 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

32796

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

Q94981

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0017418

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

ari-1_1

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG5659

Flygut - An atlas of the Drosophila adult midgut

FBgn0017418

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

32796

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG5659

modMine - A data warehouse for the modENCODE project

FBgn0017418

Linkouts

BioGRID - A database of protein and genetic interactions.

59116

DroID - A comprehensive database of gene and protein interactions.

FBgn0017418

DRSC - Results frm RNAi screens

FBgn0017418

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0017418

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG5659-RA

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0017418

FlyMine - An integrated database for Drosophila genomics

FBgn0017418

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

32796

MIST (genetic) - An integrated Molecular Interaction Database

ari-1

MIST (protein-protein) - An integrated Molecular Interaction Database

ari-1

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

ISG15 antiviral mechanism

Synonyms and Secondary IDs (13)

Reported As

Symbol Synonym

AAS65398.1

(Eisenhaber et al., 2007)

ARI1_DROME

(Storm and Sonnhammer, 2003)

Ari-1

(Azevedo and Baylies, 2020, Oriel and Lasko, 2018, Ying et al., 2011, Gradilla and Ferrus, 2004)

CG5659

(Haelterman et al., 2014, Ying et al., 2011, Perkins et al., 2009, Misra, 2001.8.16, Mount, 2001.8.14)

HL-V

(Ferrus, 1999.3.4, Prado et al., 1999)

ari

(Moynihan et al., 1999, Cowley, 1998.9.24, de la Pompa, 1994, Ferrus et al., 1990)

ari-1

(Meltzer et al., 2019, Story et al., 2019, Baranski et al., 2018, Tan et al., 2018, Ketosugbo et al., 2017, Eliazer et al., 2014, Gonzalez et al., 2014, Haelterman et al., 2014, McPhee et al., 2013, Vaque et al., 2013, Du et al., 2011, Langille and Clark, 2007, Kuo et al., 2006, Martin et al., 2006, Gleason, 2005)

ari-1a

(Gradilla et al., 2011)

l(1)16Fj

Name Synonyms

Ariadne

(Felix et al., 2012)

Ariadne-1

(Oriel and Lasko, 2018, Gradilla and Ferrus, 2004)

ariadne

(Dason et al., 2009, Gleason, 2005, Barbas, 1996.6.5, Barbas, 1996.6.5)

ariadne 1

Secondary FlyBase IDs

FBgn0014203

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

References (64)

The following external sites may use different assemblies or annotations than FlyBase.

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