Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\SAR using the Feature Mapper tool.
GBrowse - Visual display of RNA-Seq signalsView Dmel\SAR in GBrowse 2
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
The 12 restriction fragments with strong SAR activity (7 of which also show ARS activity) in an 830kb region of the Drosophila X chromosome show a statistically strong coincidence or contiguity with regions of high topoisomerase II cleavage site frequency.
11 anchorage sites delimiting 10 DNA loops have been mapped in a 500kb region of the D.melanogaster X chromosome. 10 of these sites co-localise with previously mapped SARs, although a number of other SARs are located in loop DNA.
SARs are demonstrated to have a stimulatory effect on gene expression in a heterologous system. This stimulatory effect can be blocked by a relatively short GC-rich DNA sequence.
A number of subfragments of an 800kb DNA walk from the X chromosome have been tested for their ability to promote autonomously replicating sequence (ARS) activity in S.cerevisiae. 25 out of 27 identified ARSs were present on fragments previously shown to contain scaffold attachment regions (SARs), while the remaining 2 ARSs were adjacent to previously identified SARs. 16 SAR containing fragments from the chromosomal walk have been tested for association with yeast nuclear scaffolds in vitro.