roX1, roX, RNA on the X 1, yang, RNA on the X1
X chomosome associated RNA - chromatin associated non-translated RNA involved in dosage compensation - ectopically expressed roX1 and roX2 RNAs target high-affinity sites on the X chromosome in trans and, via spatial proximity, induce spreading of the MSL complex in cis, leading to increased expression of neighboring autosomal genes - The MSL complex regulates nucleosome positioning at high-affinity sites, therefore acting locally rather than influencing the overall chromosomal architecture.
Gene model reviewed during 5.48
Low-frequency RNA-Seq exon junction(s) not annotated.
Gene model reviewed during 5.56
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\lncRNA:roX1 using the Feature Mapper tool.
In adult males, roX1 transcripts are expressed in the cell bodies of the central brain and mushroom bodies, the neurons of the optic lobe, and the interneurons of the antennal lobes. They are also expressed in peripheral neurons including the photoreceptor cells and the olfactory neurons of the third antennal segment. Strong expression is observed in the thoracic ganglia and weak expression is seen in the gut. Expression appears to be confined to the nucleus. No expression is observed in females.
In cryosections of adults, roX1 transcription is strongest in the central nervous system of the male brain. Most cells of the brain and thoracic ganglia stain strongly. Weaker expression is observed in the gut, parts of the reproductive tract (especially the ejaculatory bulb), and in fat cells. In embryos, roX1 transcripts are expressed in both sexes starting at about 2.5 hours after egg laying. Expression is strong in cells of the CNS until ~stage 13 at which point the expression in females subsides. The staining disappears first from the ventral nerve cord and then from the brain. In male larvae, strong staining is seen in many tissues, including the large polyploid cells of the gastric caecae, the ring gland and the nephrocytes, and the salivary glands. This staining is nuclear and localizes to the X chromosome. roX1 expression is controlled by the dosage compensation pathway and not by tra.
GBrowse - Visual display of RNA-Seq signalsView Dmel\lncRNA:roX1 in GBrowse 2
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Transcription of the roX1 gene is directly regulated by the DNaseI hypersensitive site (DHS) within the roX1 transcription unit. This regulation requires the msl-1, msl-2, msl-3 and mle proteins but not roX (roX1 and roX2) RNAs. The DHS is not required for initiation of cis spreading of the MSL complex.
New annotation (CR32777) in release 3 of the genome annotation.
The "roX1 chromatin entry site" (roX1-CES) is a region within the roX1 gene that is able to recruit the MSL complex. A 217bp fragment is sufficient for recruitment and spreading of the MSL complex along the flanking chromatin. This short element is sufficient to activate MSL spreading in the absence of active roX1 transcription.
The roX1 gene contains sequences that seem to provide an entry site for the MSL complex to recognize the X chromosome. This site is termed the "roX1 chromatin entry site" (roX1-CES). The roX1-CES provides a nucleation site for extensive spreading (in cis or trans) of the complex into flanking chromatin, even when moved to an autosome.
roX1 encodes an RNA with no apparent reading frame that shows male-specific expression in the adult fly, and is regulated by genes of the dosage compensation pathway. It is closely linked to yin, a female-specific gene expressed in the ovary.