Please see the JBrowse view of Dmel\dj for information on other features
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AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.
Gene model reviewed during 5.50
Multiphase exon postulated: reading frame of first coding exon differs in alternative transcripts.
1.3 (northern blot)
None of the polypeptides share 100% sequence identity.
248 (aa); 32 (kD observed); 29.2 (kD predicted)
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\dj using the Feature Mapper tool.
The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).
dj transcript is expressed in male germ cells. A djFBtr0091350:pb-XREcollacZ construct was used to distinguish dj protein and transcript expression in the male germline. EcollacZ transcript was expressed only in premeiotic cells, while the EcollacZ protein was detected only postmeiotically, in elongated sperm bundles. Similarly, a djFBtr0091350:pb-XRGFP fusion protein was detected only in individualized sperm.
In adult testis, expression is restricted to fully elongated spermatids and is associated primarily with the spermatid axonemes. In the testis, no GFP fluorescence observed in premeiotic cells.
A 32 kD dj protein was detected in Western blots of testis extracts. A djFBtr0091350:pb-XRlacZ construct was used to distinguish dj protein and transcript expression in the male germline. lacZ transcript was expressed only in premeiotic cells, while the lacZ protein was detected only postmeiotically, in elongated sperm bundles. Similarly, a djFBtr0091350:pb-XRGFP fusion protein was detected only in individualized sperm.
JBrowse - Visual display of RNA-Seq signals
View Dmel\dj in JBrowse3-48
3-44.2
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Isolated from a Drosophila testis cDNA expression library, using the regulatory upstream sequence of the βTub85D promoter as a probe.
Source for identity of: dj CG1980
