FlyBase Gene Report: Dmel\Ran

General Information

Symbol

Dmel\Ran

Species

D. melanogaster

Name

Ran

Annotation Symbol

CG1404

Feature Type

protein_coding_gene

FlyBase ID

FBgn0020255

Gene Model Status

Current

Stock Availability

7 publicly available

Gene Snapshot

Contributions welcome

Other Summaries

Alliance
Auto summary
Gene Group
UniProtKB
Interactive Fly

Also Known As

l(1)G0075, Ran GTPase

Key Links

Genomic Location

Cytogenetic map

10A8-10A8

Sequence location

X:11,144,852..11,148,716 [-]

Recombination map

1-33

RefSeq locus

NC_004354 REGION:11144852..11148716

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse GBrowse

Help me choose

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

Gene Ontology (GO) Annotations (17 terms)

Molecular Function (3 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

enables GTP binding

inferred from direct assay

(assigned by UniProt )

(Trieselmann and Wilde, 2002)

enables protein binding

inferred from physical interaction with UniProtKB:Q9W1A4

(assigned by UniProt )

(Minakhina et al., 2003)

inferred from physical interaction with FLYBASE:cno; FB:FBgn0259212

(Wee et al., 2011)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

enables GTPase activity

inferred from biological aspect of ancestor with PANTHER:PTN000632582

(assigned by GO_Central )

(Gaudet et al., 2011)

inferred from sequence or structural similarity with SGD:S000005711

(FlyBase Curators, 2008--)

Biological Process (9 terms)

Terms Based on Experimental Evidence (5 terms)

CV Term

Evidence

References

involved_in establishment of mitotic spindle orientation

inferred from mutant phenotype

(Wee et al., 2011)

inferred from genetic interaction with FLYBASE:dlg1; FB:FBgn0001624

(Wee et al., 2011)

inferred from genetic interaction with FLYBASE:cno; FB:FBgn0259212

(Wee et al., 2011)

involved_in meiotic spindle organization

inferred from mutant phenotype

(Cesario and McKim, 2011)

involved_in nuclear pore complex assembly

inferred from mutant phenotype

(assigned by UniProt )

(Hampoelz et al., 2019)

involved_in regulation of mitotic cell cycle

inferred from direct assay

(Trieselmann and Wilde, 2002)

involved_in regulation of protein localization

inferred from mutant phenotype

(Wee et al., 2011)

Terms Based on Predictions or Assertions (4 terms)

CV Term

Evidence

References

involved_in intracellular protein transport

traceable author statement

(assigned by UniProt )

(Trieselmann and Wilde, 2002)

involved_in protein export from nucleus

inferred from sequence or structural similarity with HGNC:9846

(FlyBase Curators, 2008--)

involved_in protein import into nucleus

inferred from biological aspect of ancestor with PANTHER:PTN000632582

(assigned by GO_Central )

(Gaudet et al., 2011)

involved_in ribosomal subunit export from nucleus

inferred from biological aspect of ancestor with PANTHER:PTN000632582

(assigned by GO_Central )

(Gaudet et al., 2011)

Cellular Component (5 terms)

Terms Based on Experimental Evidence (5 terms)

CV Term

Evidence

References

located_in cell cortex

inferred from direct assay

(Wee et al., 2011)

located_in cytoplasm

inferred from direct assay

(assigned by UniProt )

(Minakhina et al., 2003)

located_in mitotic spindle microtubule

inferred from direct assay

(assigned by UniProt )

(Trieselmann and Wilde, 2002)

colocalizes_with mitotic spindle

inferred from direct assay

(Cesario and McKim, 2011)

located_in nucleus

inferred from direct assay

(Cesario and McKim, 2011)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

located_in cytoplasm

inferred from biological aspect of ancestor with PANTHER:PTN000632582

(assigned by GO_Central )

(Gaudet et al., 2011)

located_in nucleus

inferred from biological aspect of ancestor with PANTHER:PTN000632582

(assigned by GO_Central )

(Gaudet et al., 2011)

Gene Group (FlyBase)

RAN GTPASES

Protein Family (UniProt)

Belongs to the small GTPase superfamily. Ran family. (Q9VZ23)

Protein Signatures (InterPro)

Summaries

Gene Group (FlyBase)

RAN GTPASES -: Ran (Ras-like nuclear) GTPases are members of the Ras superfamily of small GTPases. Ran GTPases are principally involved in the nucleocytoplasmic transport of RNA and protein. (Adapted from PMID:15731001).

Protein Function (UniProtKB)

GTPase involved in nucleocytoplasmic transport, participating both to the import and the export from the nucleus of proteins and RNAs. Switches between a cytoplasmic GDP- and a nuclear GTP-bound state by nucleotide exchange and GTP hydrolysis. Nuclear import receptors such as importin beta bind their substrates only in the absence of GTP-bound RAN and release them upon direct interaction with GTP-bound RAN, while export receptors behave in the opposite way. Thereby, RAN controls cargo loading and release by transport receptors in the proper compartment and ensures the directionality of the transport. Interaction with RANBP1 induces a conformation change in the complex formed by XPO1 and RAN that triggers the release of the nuclear export signal of cargo proteins. RAN (GTP-bound form) triggers microtubule assembly at mitotic chromosomes and is required for normal mitotic spindle assembly and chromosome segregation. Required for normal progress through mitosis (By similarity). GTP-bound Ran modulates both spindle and nuclear envelope assembly, supporting a role during mitosis (PubMed:12121620). During oogenesis, modulates formation of Nup358-containing granules and biogenesis of the nuclear pore complex probably by mediating the transport of their components along microtubules (PubMed:31626769).

(UniProt, Q9VZ23)

Summary (Interactive Fly)

Ran GTPase activity required nucleo-cytoplasmic transport - also required for microtubule organization during mitosis and for alignment of chromosomes at the metaphase plate

Interactive Fly

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Ran for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Comments on Gene Model

Gene model reviewed during 5.51

Annotated transcripts do not represent all supported alternative splices within 5' UTR.

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.50

Gene model reviewed during 5.56

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Ran-RA

FBtr0073472

NM_143712

1667

216

Ran-RB

FBtr0073471

NM_167272

1127

216

Ran-RC

FBtr0340164

NM_001298182

1581

216

Ran-RD

FBtr0340165

NM_001298183

1263

216

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

Ran-PA

FBpp0073328

24.7

216

7.96

NP_651969

AAF48008

Ran-PB

FBpp0073327

24.7

216

7.96

NP_727499

AAN09287

Ran-PC

FBpp0309150

24.7

216

7.96

NP_001285111

AHN59581

Ran-PD

FBpp0309151

24.7

216

7.96

NP_001285112

AHN59582

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

216 aa isoforms: Ran-PA, Ran-PB, Ran-PC, Ran-PD

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Subunit Structure (UniProtKB)

Found in a nuclear export complex with RanGTP, exportin and pre-miRNA (By similarity). Interacts with tamo (PubMed:12653959).

(UniProt, Q9VZ23)

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Ran using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Ran_1

Linkouts

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 7 -- 8

head mesoderm

(Fisher et al., 2012)

organism | ubiquitous

(Fisher et al., 2012)

trunk mesoderm

(Fisher et al., 2012)

ventral nerve cord primordium

Comment: reported as ventral nerve cord anlage

(Fisher et al., 2012)

embryonic stage 9 -- 10

antennal primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)

central brain primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)

visual primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)

dorsal head epidermis primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)

lateral head epidermis primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)

ventral head epidermis primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)

head mesoderm

(Fisher et al., 2012)

organism | ubiquitous

(Fisher et al., 2012)

trunk mesoderm

(Fisher et al., 2012)

ventral nerve cord primordium

(Fisher et al., 2012)

embryonic stage 13 -- 16

embryonic brain

(Fisher et al., 2012)

embryonic central brain neuron

(Fisher et al., 2012)

embryonic central brain surface glia

(Fisher et al., 2012)

embryonic hindgut

(Fisher et al., 2012)

embryonic Malpighian tubule

(Fisher et al., 2012)

embryonic/larval midgut

(Fisher et al., 2012)

embryonic/larval nervous system

(Fisher et al., 2012)

mushroom body primordium

Comment: reported as embryonic central brain mushroom body

(Fisher et al., 2012)

organism | faint | ubiquitous

(Fisher et al., 2012)

ventral midline of embryo

(Fisher et al., 2012)

ventral nerve cord

(Fisher et al., 2012)

embryonic stage 9 -- 12

midline primordium | subset

(Kearney et al., 2004)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

distribution deduced from reporter or direct label

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

organism

• spindle

Comment: mitosis

(Trieselmann and Wilde, 2002)

organism

• nucleus & nuclear membrane

Comment: interphase

(Trieselmann and Wilde, 2002)

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

day 7 of adulthood -- day 49 of adulthood

adult heart

(Cammarato et al., 2011)

adult stage

adult head

• membrane

(Aradska et al., 2015)

Additional Descriptive Data

Expression of ranprotein was inferred by the localization of human rhodamine labeled Ran injected into embryos. The protein was partially excluded from the chromatin. Based on the localization of mutant proteins locked into either the GTP or GDP bound states it was concluded that the majority of the labeled ran protein injected into the embryo was bound to GTP.

(Trieselmann and Wilde, 2002)

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in cell cortex

inferred from direct assay

(Wee et al., 2011)

located_in cytoplasm

inferred from direct assay

(assigned by UniProt )

(Minakhina et al., 2003)

located_in mitotic spindle microtubule

inferred from direct assay

(assigned by UniProt )

(Trieselmann and Wilde, 2002)

colocalizes_with mitotic spindle

inferred from direct assay

(Cesario and McKim, 2011)

located_in nucleus

inferred from direct assay

(Cesario and McKim, 2011)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Ran in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Ran

View exonic expression by tissue for Dmel\Ran

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0020255

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0020255

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG1404-RB;CG14991-RA

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG1404

Flygut - An atlas of the Drosophila adult midgut

FBgn0020255

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\Ran

Stages(s) 1-3
Stages(s) 4-6
Stages(s) 7-8
Stages(s) 9-10
Stages(s) 11-12
Stages(s) 13-16

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 2 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 12 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Ran

Transgenic constructs containing regulatory region of Ran

Aberrations (Deficiencies and Duplications) ( 1 )

Inferred from experimentation

Gene duplicated in

Dp(1;3)DC235

(Popodi et al., 2010-, Venken et al., 2010)

Inferred from location

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal, with Scer\GAL4^Mef2.PR

Ran^GD8203

lethal, with Scer\GAL4^pnr-MD237

Ran^GD8203

lethal, with Scer\GAL4^αTub84B.PL

lethal - all die before end of larval stage, with Scer\GAL4^elav.PLu

Ran^{G19V.Q69L.UAS}

lethal - all die before end of larval stage, with Scer\GAL4^h-1J3

Ran^{G19V.Q69L.UAS}

lethal - all die before end of larval stage, with Scer\GAL4^nub.PK

Ran^{G19V.Q69L.UAS}

lethal - all die before end of P-stage

Ran^G0075

lethal - all die before end of P-stage, with Scer\GAL4^pros.PMG

Ran^{G19V.Q69L.UAS}

partially lethal - majority die | recessive

Ran^G0075

viable

Ran^EY05705

viable, with Scer\GAL4^elav.PLu

Ran^UAS.cKa

viable, with Scer\GAL4^h-1J3

Ran^UAS.cKa

viable, with Scer\GAL4^nub.PK

Ran^UAS.cKa

viable, with Scer\GAL4^pros.PMG

Ran^UAS.cKa

viable, with Scer\GAL4^tin.CΔ4

Ran^GD8203

viable, with Scer\GAL4^unspecified

viable, with Scer\GAL4^αTub84B.PL

Ran^UASp.Tag:HA

Sterility

Allele

female sterile, with Scer\GAL4^VP16.nos.UTR

fertile

Ran^EY05705

fertile, with Scer\GAL4^VP16.nos.UTR

Ran^UASp.Tag:HA

male sterile, with Scer\GAL4^VP16.nos.UTR

Ran^{T24N.UASp.Tag:HA}

Other Phenotypes

Allele

some die during larval stage, with Scer\GAL4^elav.PLu

Ran^{G19V.Q69L.UAS}

some die during larval stage, with Scer\GAL4^h-1J3

Ran^{G19V.Q69L.UAS}

some die during larval stage, with Scer\GAL4^nub.PK

Ran^{G19V.Q69L.UAS}

some die during pharate adult stage

Ran^G0075

some die during pharate adult stage, with Scer\GAL4^pros.PMG

Ran^{G19V.Q69L.UAS}

visible

Ran^{T24N.UASp.Tag:HA}

visible, with Scer\GAL4^sev.EP.M

Ran^{G19V.Q69L.UAS}

wild-type, with Scer\GAL4^dpp.blk1

wild-type, with Scer\GAL4^GMR.PF

Ran^UAS.cKa

wild-type, with Scer\GAL4^sev.EP.M

Ran^UAS.cKa

Phenotype manifest in

Allele

annulate lamellae | oogenesis, with Scer\GAL4^VP16.nos.UTR

cone cell, with Scer\GAL4^GMR.PF

Ran^{G19V.Q69L.UAS}

eye

Ran^{T24N.UASp.Tag:HA}

karyosome, with Scer\GAL4^VP16.nos.UTR

meiotic spindle, with Scer\GAL4^VP16.nos.UTR

ommatidium, with Scer\GAL4^sev.EP.M

Ran^{G19V.Q69L.UAS}

oocyte, with Scer\GAL4^VP16.nos.UTR

photoreceptor cell, with Scer\GAL4^GMR.PF

Ran^{G19V.Q69L.UAS}

photoreceptor cell, with Scer\GAL4^sev.EP.M

Ran^{G19V.Q69L.UAS}

photoreceptor cell R7, with Scer\GAL4^sev.EP.M

Ran^{G19V.Q69L.UAS}

pigment cell, with Scer\GAL4^GMR.PF

Ran^{G19V.Q69L.UAS}

Orthologs

Downloads

Download All DIOPT Orthologs

Download All OrthoDB Orthologs

Human Orthologs (via DIOPT v8.0)

Homo sapiens (Human) (1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Hsap\RAN

13 of 15

Yes

Model Organism Orthologs (via DIOPT v8.0)

Mus musculus (laboratory mouse) (3)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Mmus\Ran

13 of 15

Yes

Mmus\1700009N14Rik

9 of 15

Yes

Mmus\Rasl2-9

9 of 15

Yes

Rattus norvegicus (Norway rat) (3)

Rnor\Ran

11 of 13

Yes

Rnor\Rasl2-9

10 of 13

Yes

Rnor\RGD1306195

10 of 13

Yes

Xenopus tropicalis (Western clawed frog) (1)

Xtro\ran

10 of 12

Yes

Danio rerio (Zebrafish) (1)

Drer\ran

13 of 15

Yes

Caenorhabditis elegans (Nematode, roundworm) (1)

Cele\ran-1

13 of 15

Yes

Arabidopsis thaliana (thale-cress) (4)

Atha\RAN2

9 of 9

Yes

Atha\RAN3

9 of 9

Yes

Atha\RAN-1

8 of 9

Yes

Atha\RAN4

3 of 9

Yes

Saccharomyces cerevisiae (Brewer's yeast) (2)

Scer\GSP1

13 of 15

Yes

Scer\GSP2

13 of 15

Yes

Schizosaccharomyces pombe (Fission yeast) (1)

Spom\spi1

11 of 12

Yes

Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG09190EQS )

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila suzukii

Spotted wing Drosophila

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA12719

Drosophila persimilis

Dper\GL20255

Drosophila willistoni

Dwil\GK17213

Drosophila virilis

Dvir\GJ16931

Drosophila mojavensis

Dmoj\GI15278

Drosophila grimshawi

Dgri\GH24573

Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG09150A6U )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Musca domestica

House fly

Mdoa\17004702

Glossina morsitans

Tsetse fly

Gmor\GMOY000905

Glossina morsitans

Tsetse fly

Gmor\GMOY004834

Lucilia cuprina

Australian sheep blowfly

Lcup\FF38_04276

Mayetiola destructor

Hessian fly

Mdes\Mdes004218

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL009287

Anopheles darlingi

American malaria mosquito

Adar\ADAC004927

Anopheles gambiae

Malaria mosquito

Agam\AGAP007699

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ008595

Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W0BHT )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Bombyx mori

Silkmoth

Bmor\BGIBMGA006751

Danaus plexippus

Monarch butterfly

Dple\DPOGS212140-PA

Heliconius melpomene

Postman butterfly

Hmel\HMEL007729

Apis florea

Little honeybee

Aflo\2163579940

Apis mellifera

Western honey bee

Amel\ran

Bombus impatiens

Common eastern bumble bee

Bimp\BIMP22896

Bombus terrestris

Buff-tailed bumblebee

Bter\15210502

Linepithema humile

Argentine ant

Lhum\LH10986

Megachile rotundata

Alfalfa leafcutting bee

Mrot\MROTU:0000fa

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG009302

Dendroctonus ponderosae

Mountain pine beetle

Dpod\YQE_04346

Tribolium castaneum

Red flour beetle

Tcas\TC012793

Pediculus humanus

Human body louse

Phum\PHUM095420

Rhodnius prolixus

Kissing bug

Rpro\RPRC011075

Cimex lectularius

Bed bug

Clec\CLEC011878

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI004024

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_01785

Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X0BFF )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strigamia maritima

European centipede

Smar\SMAR004591

Ixodes scapularis

Black-legged tick

Isca\ISCW023654

Ixodes scapularis

Black-legged tick

Isca\ISCW021828

Stegodyphus mimosarum

African social velvet spider

Smim\KFM80570.1

Tetranychus urticae

Two-spotted spider mite

Turt\tetur27g01880

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_305970

Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G0L3X )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Ran

Ciona intestinalis

Vase tunicate

Cint\ENSCING00000003944

Gallus gallus

Domestic chicken

Ggal\RAN

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v8.0)

Drosophila melanogaster (Fruit fly) (1)

Gene Symbol

Score

Source

Alignment

4 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Allele

Disease

Interaction

References

Ran^{T24N.UASp.Tag:HA}

exacerbates amyotrophic lateral sclerosis

modeled by Zzzz\GGGGCC^58.UAS.EGFP

(Freibaum et al., 2015)

exacerbates frontotemporal dementia

modeled by Zzzz\GGGGCC^58.UAS.EGFP

(Freibaum et al., 2015)

Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

RAN; RAN, member RAS oncogene family

13 of 15

601179

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

View in Interactions Browser

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Ran - CG3342

two hybrid

(Hu et al., 2017.6.13)

Ran - Gsc

two hybrid

(Hu et al., 2017.6.13)

Ran - Mer

anti tag coimmunoprecipitation, peptide massfingerprinting

(Kwon et al., 2013)

Ran - cno

anti tag coimmunoprecipitation, anti tag western blot, pull down, western blot

(Wee et al., 2011)

Ran - ex

anti tag coimmunoprecipitation, peptide massfingerprinting

(Kwon et al., 2013)

Ran - hpo

anti tag coimmunoprecipitation, peptide massfingerprinting

(Kwon et al., 2013)

Ran - sav

anti tag coimmunoprecipitation, peptide massfingerprinting

(Kwon et al., 2013)

Ran - tamo

pull down, autoradiography

(Minakhina et al., 2003)

Ran - tim

pull down, western blot

(Jang et al., 2015)

Ran - vtd

anti bait coimmunoprecipitation, Identification by mass spectrometry, anti tag coimmunoprecipitation

(Ribeiro et al., 2016)

RNA-protein

Physical Interaction

Assay

References

Ran - hpRNA:CR46342

anti tag coimmunoprecipitation, northern blot

(Zhou et al., 2008)

Ran - mir-ban

anti tag coimmunoprecipitation, northern blot

(Zhou et al., 2008)

Summary of Genetic Interactions

esyN Network Diagram

esyN Network Key:

Suppression

Enhancement

View in Interactions Browser

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Ran

suppressible

sub

(Cesario and McKim, 2011)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

RanGAP

suppressible

Ran

(Kusano et al., 2001)

sub

suppressible

Ran

(Cesario and McKim, 2011)

External Data

Subunit Structure (UniProtKB)

Found in a nuclear export complex with RanGTP, exportin and pre-miRNA (By similarity). Interacts with tamo (PubMed:12653959).

(UniProt, Q9VZ23 )

Linkouts

BioGRID - A database of protein and genetic interactions.

68814

DroID - A comprehensive database of gene and protein interactions.

FBgn0020255

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

44072

MIST (genetic) - An integrated Molecular Interaction Database

MIST (protein-protein) - An integrated Molecular Interaction Database

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

External Data

Linkouts

KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

1-33

Cytogenetic map

10A8-10A8

Sequence location

X:11,144,852..11,148,716 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

10A8-10A8

Limits computationally determined from genome sequence between P{EP}EP1321^EP1321&P{EP}CG2061^EP1537 and P{EP}CG11756^EP1610

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

10A7-10A9

(determined by in situ hybridisation)

(Koizumi et al., 2001)

10A-10A

(Frasch, 2000.2.11)

10A4-10A9

(determined by in situ hybridisation)

(Schäfer et al., 1999.6.15)

9D-9D

(determined by in situ hybridisation)

(Schäfer et al., 1999.3.29)

10A7-10A9

(Stivers et al., 1997)

Experimentally Determined Recombination Data

Location

1-33

(FlyBase, 2016)

1-34.8

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (7)

Bloomington

11800

w^67c23 P{lacW}Ran^G0075/FM7c

31392

y¹ v¹; P{TRiP.JF01381}attP2

16670

y¹ w^67c23 P{EPgy2}Ran^EY05705

42482

y¹ sc^* v¹ sev²¹; P{TRiP.GL01341}attP40

44587

y¹ sc^* v¹ sev²¹; P{TRiP.HMS02885}attP2

VDRC

v104417

P{KK108549}VIE-260B

v24835

w¹¹¹⁸; P{GD8203}v24835/CyO

Genomic Clones (14)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (302)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

DPiM_GM14354

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0020255

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0020255

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

44072

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for identity of: Ran ran

(FlyBase, 2009-)

Source for database merge of

Source for merge of: ran CG1404

(Bayraktaroglu, 2000.8.7)

Source for merge of: ran l(1)G0075

(Peter et al., 2002)

Additional comments

The ran-like gene may have been derived from the ran gene by retroposition.

(Dai et al., 2006)

Other Comments

Expression is enriched in embryonic gonads.

(Shigenobu et al., 2006)

dsRNA made from templates generated with primers directed against ran that is transfected into S2 treated with Listeria monocytogenes reveals ran to be involved in Listeria monocytogenes entry and vacuolar escape.

(Cheng et al., 2005)

dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R⁺ cell morphology.

(Kiger et al., 2003)

RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a phenotype when assayed in Kc167 cells: change from round to spindle-shaped, with the formation of F-actin puncta and microtubule extensions. Alterations of cell shape are also evident in S2R+ cells; cells become round and detached.

(Kiger et al., 2003)

Identification: Screen for genes required for late lineage CNS development.

(Stivers et al., 1997)

Origin and Etymology

Discoverer

Etymology

Identification

External Crossreferences and Linkouts ( 61 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

44072

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

Q9VZ23

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

A4V4A5

Other crossreferences

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0020255

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0020255

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Ran_1

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG1404

Flygut - An atlas of the Drosophila adult midgut

FBgn0020255

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

44072

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG1404

modMine - A data warehouse for the modENCODE project

FBgn0020255

Linkouts

BioGRID - A database of protein and genetic interactions.

68814

DPiM - Drosophila Protein interaction map

FBgn0020255

DroID - A comprehensive database of gene and protein interactions.

FBgn0020255

DRSC - Results frm RNAi screens

FBgn0020255

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0020255

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG1404-RB;CG14991-RA

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0020255

FlyMine - An integrated database for Drosophila genomics

FBgn0020255

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

44072

KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.

MIST (genetic) - An integrated Molecular Interaction Database

MIST (protein-protein) - An integrated Molecular Interaction Database

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Synonyms and Secondary IDs (11)

Reported As

Symbol Synonym

AAF30287

(Jekely, 2003)

Q9VZ23

(Rojas et al., 2012)

RanGTP

(Wee et al., 2011)

dran

(Stivers et al., 1997)

l(1)G0075

(Schäfer et al., 1999.3.29, Schäfer et al., 1999.6.15)

ran10A

(Frasch, 2000.2.11)

Name Synonyms

Ran

Ran GTPase

(Freibaum et al., 2015, Sepp et al., 2008)

ran

(Cammarato et al., 2011, Somma et al., 2008)

ran GTPase

(Lindquist et al., 2011)

Secondary FlyBase IDs

FBgn0026685
FBgn0030273

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

References (125)

The following external sites may use different assemblies or annotations than FlyBase.

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