FlyBase Gene Report: Dmel\E23

General Information

Symbol

Dmel\E23

Species

D. melanogaster

Name

Early gene at 23

Annotation Symbol

CG3327

Feature Type

protein_coding_gene

FlyBase ID

FBgn0020445

Gene Model Status

Current

Stock Availability

9 publicly available

Gene Snapshot

Contributions welcome

Other Summaries

Alliance
Auto summary
Gene Group
Interactive Fly

Key Links

Genomic Location

Cytogenetic map

23E4-23E5

Sequence location

2L:3,334,875..3,354,829 [-]

Recombination map

2-9

RefSeq locus

NT_033779 REGION:3334875..3354829

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse GBrowse

Help me choose

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

Gene Ontology (GO) Annotations (6 terms)

Molecular Function (3 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

enables ATP binding

inferred from electronic annotation with InterPro:IPR003439, InterPro:IPR017871

(assigned by InterPro )

(FlyBase Curators et al., 2004-)

enables ATPase activity

inferred from electronic annotation with InterPro:IPR003439, InterPro:IPR017871

(assigned by InterPro )

(FlyBase Curators et al., 2004-)

enables ATPase-coupled transmembrane transporter activity

inferred from sequence or structural similarity with UniProtKB:P10090

(FlyBase Curators, 2008--)

Biological Process (2 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

involved_in regulation of circadian rhythm

inferred from direct assay

(Itoh et al., 2011)

inferred from mutant phenotype

(Matsumoto et al., 2007)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

involved_in transmembrane transport

inferred from sequence or structural similarity with UniProtKB:P10090

(FlyBase Curators, 2008--)

Cellular Component (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

located_in plasma membrane

inferred from biological aspect of ancestor with PANTHER:PTN000443095

(assigned by GO_Central )

(Gaudet et al., 2011)

Gene Group (FlyBase)

ABCG ATP-BINDING CASSETTE TRANSPORTER SUBFAMILY

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Gene Group (FlyBase)

ABCG ATP-BINDING CASSETTE TRANSPORTER SUBFAMILY -: The ATP-binding cassette (ABC) transporter family are primary active transporters that use ATP hydrolysis to drive the transport of substrates across the membrane. In metazoans the ABCG subfamily are half transporters. Half transporters must dimerize to form a functional transporter. (Adapted from FBrf0224053).

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\E23 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Comments on Gene Model

Gene model reviewed during 5.49

Annotated transcripts do not represent all supported alternative splices within 5' UTR.

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.53

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

E23-RC

FBtr0089979

NM_205900

4514

1017

E23-RD

FBtr0339548

NM_079909

4397

1017

E23-RE

FBtr0339549

NM_134924

5149

1017

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

E23-PC

FBpp0088917

112.4

1017

7.23

NP_995622

AAS64617

E23-PD

FBpp0308631

112.4

1017

7.23

NP_524648

AAF51121

E23-PE

FBpp0308632

112.4

1017

7.23

NP_608768

AAF51122

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

1017 aa isoforms: E23-PC, E23-PD, E23-PE

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\E23 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

E23_1

Linkouts

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\E23 in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\E23

View exonic expression by tissue for Dmel\E23

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0020445

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG3327-RA

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG3327

Flygut - An atlas of the Drosophila adult midgut

FBgn0020445

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 13 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 10 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of E23

Transgenic constructs containing regulatory region of E23

Aberrations (Deficiencies and Duplications) ( 0 )

Inferred from experimentation

Inferred from location

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal, with Dcr-2^UAS.cDa, Scer\GAL4^e22c, Scer\GAL4^sqh.PW

E23^KK113210

viable, with Dcr-2^UAS.cDa, Scer\GAL4^e22c, Scer\GAL4^sqh.PW

E23^GD866

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

E23^GD866

viable, with Scer\GAL4^Mef2.PR

E23^GD866

viable, with Scer\GAL4^pnr-MD237

E23^GD866

viable, with Scer\GAL4^tin.CΔ4

E23^GD866

Other Phenotypes

Allele

abnormal circadian rhythm, with Scer\GAL4^P2.4.Pdf

abnormal locomotor rhythm, with Scer\GAL4^P2.4.Pdf

E23^dsRNA.UAS

abnormal locomotor rhythm, with Scer\GAL4^tim.UAS

E23^dsRNA.UAS

Phenotype manifest in

Allele

Orthologs

Downloads

Download All DIOPT Orthologs

Download All OrthoDB Orthologs

Human Orthologs (via DIOPT v8.0)

Homo sapiens (Human) (18)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Hsap\ABCG1

3 of 15

Yes

Hsap\ABCG2

3 of 15

Yes

Hsap\ABCG5

3 of 15

Yes

Hsap\ABCG8

3 of 15

Yes

2 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Model Organism Orthologs (via DIOPT v8.0)

Mus musculus (laboratory mouse) (23)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Mmus\Abcg1

3 of 15

Yes

Mmus\Abcg2

3 of 15

Yes

Mmus\Abcg5

3 of 15

Yes

Mmus\Abcg8

3 of 15

Yes

2 of 15

2 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Rattus norvegicus (Norway rat) (24)

Rnor\Abcg1

3 of 13

Yes

Rnor\Abcg2

3 of 13

Yes

Rnor\Abcg3l1

3 of 13

Yes

Rnor\Abcg5

3 of 13

Yes

Rnor\Abcg3l3

2 of 13

Rnor\Abcg3l4

2 of 13

Yes

Rnor\Abcg3

2 of 13

Yes

2 of 13

2 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Rnor\LOC103690063

1 of 13

Yes

Xenopus tropicalis (Western clawed frog) (12)

Xtro\abcg2

3 of 12

Yes

Xtro\abcg5

3 of 12

Yes

Xtro\abcg8

3 of 12

Yes

Xtro\abcg1

2 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Danio rerio (Zebrafish) (15)

Drer\abcg1

3 of 15

Yes

Drer\abcg2a

3 of 15

Yes

Drer\abcg2b

3 of 15

Yes

Drer\abcg2c

3 of 15

Yes

Drer\abcg5

3 of 15

Yes

Drer\abcg8

3 of 15

Yes

2 of 15

2 of 15

2 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Caenorhabditis elegans (Nematode, roundworm) (18)

Cele\wht-4

4 of 15

Yes

3 of 15

3 of 15

3 of 15

3 of 15

3 of 15

3 of 15

2 of 15

2 of 15

2 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Arabidopsis thaliana (thale-cress) (24)

Atha\ABCG22

7 of 9

Yes

Atha\ABCG25

7 of 9

Yes

Atha\ABCG9

6 of 9

Yes

Atha\ABCG14

6 of 9

Yes

Atha\ABCG21

6 of 9

Yes

Atha\ABCG26

6 of 9

Yes

Atha\ABCG27

6 of 9

Yes

2 of 9

2 of 9

2 of 9

2 of 9

2 of 9

2 of 9

2 of 9

2 of 9

2 of 9

2 of 9

2 of 9

2 of 9

2 of 9

2 of 9

2 of 9

Yes

Atha\ABCG28

2 of 9

Yes

Atha\NAP12

2 of 9

Yes

Saccharomyces cerevisiae (Brewer's yeast) (10)

Scer\ADP1

5 of 15

Yes

2 of 15

2 of 15

2 of 15

2 of 15

2 of 15

2 of 15

2 of 15

2 of 15

2 of 15

Schizosaccharomyces pombe (Fission yeast) (2)

Spom\pdr1

2 of 12

Yes

Spom\bfr1

1 of 12

Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG091902UR )

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila suzukii

Spotted wing Drosophila

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA17380

Drosophila persimilis

Dper\GL19524

Drosophila willistoni

Dwil\GK18354

Drosophila virilis

Dvir\GJ10392

Drosophila mojavensis

Dmoj\GI15918

Drosophila grimshawi

Dgri\GH10473

Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091501MJ )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Musca domestica

House fly

Mdoa\17008892

Glossina morsitans

Tsetse fly

Gmor\GMOY005277

Lucilia cuprina

Australian sheep blowfly

Lcup\FF38_13991

Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W02KX )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Apis florea

Little honeybee

Aflo\3197176868

Apis mellifera

Western honey bee

Amel\GB49098

Bombus impatiens

Common eastern bumble bee

Bimp\BIMP13037

Bombus terrestris

Buff-tailed bumblebee

Bter\15207598

Linepithema humile

Argentine ant

Lhum\LH19678

Linepithema humile

Argentine ant

Lhum\LH19680

Megachile rotundata

Alfalfa leafcutting bee

Mrot\MROTU:0003b9

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG006951

Dendroctonus ponderosae

Mountain pine beetle

Dpod\YQE_05792

Tribolium castaneum

Red flour beetle

Tcas\TC005701

Pediculus humanus

Human body louse

Phum\PHUM409050

Rhodnius prolixus

Kissing bug

Rpro\RPRC002302

Rhodnius prolixus

Kissing bug

Rpro\RPRC002303

Cimex lectularius

Bed bug

Clec\CLEC025094

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI007020

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_03289

Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X01YJ )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strigamia maritima

European centipede

Smar\SMAR003997

Ixodes scapularis

Black-legged tick

Isca\ISCW014282

Stegodyphus mimosarum

African social velvet spider

Smim\KFM57254.1

Stegodyphus mimosarum

African social velvet spider

Smim\KFM68696.1

Tetranychus urticae

Two-spotted spider mite

Turt\tetur17g02510

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_347416

Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G02ZS )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Abcg9

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Abcg2

Ciona intestinalis

Vase tunicate

Cint\ENSCING00000010518

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v8.0)

Drosophila melanogaster (Fruit fly) (28)

Gene Symbol

Score

Source

Alignment

5 of 10

4 of 10

4 of 10

4 of 10

4 of 10

4 of 10

4 of 10

4 of 10

3 of 10

3 of 10

3 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

osy

1 of 10

snu

1 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 2 )

Human Ortholog

Disease

Evidence

References

ABCG8; ATP binding cassette subfamily G member 8

model of sitosterolemia

IEA

(FlyBase, 2019-)

model of gallbladder disease

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

ABCG1; ATP binding cassette subfamily G member 1

3 of 15

603076

ABCG2; ATP binding cassette subfamily G member 2 (Junior blood group)

3 of 15

603756

ABCG5; ATP binding cassette subfamily G member 5

3 of 15

605459

SITOSTEROLEMIA 2; STSL2

ABCG8; ATP binding cassette subfamily G member 8

3 of 15

605460

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

Summary of Genetic Interactions

esyN Network Diagram

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

68699

DroID - A comprehensive database of gene and protein interactions.

FBgn0020445

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

43895

MIST (protein-protein) - An integrated Molecular Interaction Database

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

External Data

Linkouts

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-9

Cytogenetic map

23E4-23E5

Sequence location

2L:3,334,875..3,354,829 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

23E4-23E5

Limits computationally determined from genome sequence between P{lacW}Mad^k00237 and P{lacW}Pdsw^k10101

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

23E-23E

(Hock et al., 1997)

Experimentally Determined Recombination Data

Location

2-9

(FlyBase, 2016)

2-7.8

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (9)

Bloomington

25636

w¹¹¹⁸; Mi{ET1}E23^MB07029

26252

y¹ v¹; P{TRiP.JF02151}attP2

55506

y¹ w^*; Mi{MIC}E23^MI10587

60988

y¹ w^*; Mi{MIC}E23^MI15151

78756

y¹ sc^* v¹ sev²¹; P{TKO.GS02453}attP40

51675

y¹ sc^* v¹ sev²¹; P{TRiP.GLC01359}attP40

57782

y¹ sc^* v¹ sev²¹; P{TRiP.HMC04976}attP40

VDRC

v105055

P{KK113210}VIE-260B

v2620

w¹¹¹⁸; P{GD866}v2620/CyO

Genomic Clones (27)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (43)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

RE53253

Other clones

FI20292

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0020445

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0020445

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

43895

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for database merge of

Source for merge of: E23 CG15410

(Garza, 2002.2.9)

Source for merge of: E23 CG3327

(FlyBase, 1996-)

Additional comments

Source for merge of E23 CG3327 was sequence comparison ( date:000915 ).

(FlyBase, 1996-)

Other Comments

RNAi generated by PCR using primers directed to this gene causes a cell growth and viability phenotype when assayed in Kc167 and S2R⁺ cells.

(Boutros et al., 2004)

E23 corresponds to the early ecdysone puff at 23E.

(Hock et al., 1997)

Origin and Etymology

Discoverer

Etymology

Identification

External Crossreferences and Linkouts ( 49 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

43895

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones