dClk, dClock, Jrk
Gene model reviewed during 5.47
Low-frequency RNA-Seq exon junction(s) not annotated.
Gene model reviewed during 5.55
5 (northern blot)
Efficient DNA binding requires dimerization with another bHLH protein. Forms a heterodimer with Cycle.
Contains three polyglutamine repeats which could correspond to the transactivation domain. The length of the repeats is polymorphic. In the arrhythmic mutant JRK, deletion of this region leads to the loss of circadian rhythmicity and altered light response.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Clk using the Feature Mapper tool.
Expression oscillates with the circadian cycle and peaks at ZT0. The relative amount of mRNA is less in bodies compared to heads.
Expressed cyclically in the adult fat body.
RT-PCR analysis indicates that the levels of the Clk transcript cycle when in light:dark conditions, in anti-phase with the levels of the per transcript. Clk transcript levels decrease gradually from ZT02 to ZT14, and increase after that until ZT20. In conditions of constant darkness the cycling behaviour is abolished. Clk transcript levels gradually increase from CT02 until CT14 and are maintained after that until CT20.
The Clk protein is detected in the dorsal and ventral lateral (LNd and LNv) and dorsal (DN1) clock neurons in the adult brain. Western blot analysis in adult head extracts indicates that in light:dark conditions, Clk protein shows a cycling behaviour, with its peak level at ZT08. In conditions of constant darkness, cycling is dampened.
Clk protein levels undergo circadian cycling. In adult heads, protein levels reach a peak between (zeitgeber times) ZT23.5 and ZT4 and a minimum between ZT11.5 and ZT16. The time course for the accumulation of Clk mRNA and Clk protein is similar. Circadian cycling of Clk protein levels continues for at least 2 days under dark conditions.
GBrowse - Visual display of RNA-Seq signalsView Dmel\Clk in GBrowse 2
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
DNA-protein interactions: genome-wide binding profile (ChIP-chip) assayed for Clk protein in adult heads; see GEO_GSE32613 (http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE32613).
Clk makes a major contribution to the strength and amplitude of circadian rhythms.
Clk is able to induce and organise the core elements of interdependent feedback loops necessary for circadian rhythms.
Posttranscriptional mechanisms make substantial contributions to the temporal changes in the abundance of Clk protein.
Shows particularly robust cycling of transcription in adult heads, as assessed by expression analysis using high density oligonucleotide arrays with probe generated during three 12-point time course experiments over the course of 6 days. Shows significant change of expression pattern in circadian mutant background; decreased expression in per01, tim01 and increased expression in ClkJrk background.
Identified as one of 10 highest fold cycling genes as assessed by expression analysis using high density oligonucleotide arrays with probe generated from adult heads harvested over six time points over the course of a day.
Sensitization to repeated cocaine exposures, a phenomenon also seen in humans and animal models and associated with enhanced drug craving, is eliminated in flies mutant for per, dco, Clk, and cyc but not tim.
Identification: Low stringency screen using the mouse Clock gene (bHLH and PAS domains) as a probe.