FB2020_05, released Oct 14, 2020

Gene: Dmel\AMPKα

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General Information
Symbol
Dmel\AMPKα
Species
D. melanogaster
Name
AMP-activated protein kinase α subunit
Annotation Symbol
CG3051
Feature Type
protein_coding_gene
FlyBase ID
FBgn0023169
Gene Model Status
Current
Stock Availability
25 publicly available
Gene Snapshot
AMP-activated protein kinase α subunit (AMPKα) encodes the α subunit of the AMP-activated protein kinase (AMPK) complex. In general AMPK helps cells conserve energy through inhibition of energy consuming processes (fatty acid synthesis, protein synthesis). AMPK is usually active when the TOR pathway is inhibited. [Date last reviewed: 2019-03-07]
Other Summaries
Also Known As

AMPK, SNF1A, AMP-activated protein kinase, EG:132E8.2 , dAMPKα

Key Links
Genomic Location
Cytogenetic map
2A1-2A1
Sequence location
X:1,375,536..1,378,896 [+]
Recombination map

1-0.2

RefSeq locus
NC_004354 REGION:1375536..1378896
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
GO Summary Ribbons
Gene Ontology (GO) Annotations (25 terms)
Molecular Function (4 terms)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
AMP-activated protein kinase activity
inferred from direct assay
(Kim and Lee, 2015)
inferred from mutant phenotype
(Pan and Hardie, 2002)
Terms Based on Predictions or Assertions (3 terms)
CV Term
Evidence
References
ATP binding
inferred from electronic annotation with InterPro:IPR000719, InterPro:IPR002290, InterPro:IPR017441
(assigned by InterPro )
(FlyBase Curators et al., 2004-)
G protein-coupled receptor kinase activity
inferred from sequence or structural similarity
(Cassill et al., 1991)
protein serine/threonine kinase activity
inferred from biological aspect of ancestor with PANTHER:PTN000678822
(assigned by GO_Central )
(Gaudet et al., 2011)
Biological Process (16 terms)
Terms Based on Experimental Evidence (14 terms)
CV Term
Evidence
References
behavioral response to starvation
inferred from mutant phenotype
(Johnson et al., 2010)
cellular response to starvation
inferred from mutant phenotype
(Johnson et al., 2010)
dendrite morphogenesis
inferred from mutant phenotype
(Swick et al., 2013)
establishment or maintenance of epithelial cell apical/basal polarity
inferred from mutant phenotype
(Lee et al., 2007)
lipid metabolic process
inferred from mutant phenotype
(Johnson et al., 2010)
mitotic cell cycle
inferred from mutant phenotype
(Lee et al., 2007)
negative regulation of cell size
inferred from mutant phenotype
(Haack et al., 2013)
negative regulation of TORC1 signaling
inferred from mutant phenotype
(Kim and Lee, 2015)
positive regulation of axon guidance
inferred from genetic interaction with FLYBASE:Rheb; FB:FBgn0041191
(Dimitroff et al., 2012)
positive regulation of lipophagy
inferred from mutant phenotype
(Lee et al., 2018)
protein phosphorylation
inferred from mutant phenotype
(Pan and Hardie, 2002)
regulation of cell growth
inferred from mutant phenotype
(Bland et al., 2010)
regulation of digestive system process
inferred from mutant phenotype
(Bland et al., 2010)
sequestering of triglyceride
inferred from mutant phenotype
(Johnson et al., 2010)
Terms Based on Predictions or Assertions (3 terms)
CV Term
Evidence
References
intracellular signal transduction
inferred from biological aspect of ancestor with PANTHER:PTN000678822
(assigned by GO_Central )
(Gaudet et al., 2011)
protein phosphorylation
inferred from sequence or structural similarity
(Cassill et al., 1991)
inferred from biological aspect of ancestor with PANTHER:PTN000678822
(assigned by GO_Central )
(Gaudet et al., 2011)
signal transduction
inferred from sequence or structural similarity
(Cassill et al., 1991)
Cellular Component (5 terms)
Terms Based on Experimental Evidence (3 terms)
CV Term
Evidence
References
basolateral part of cell
inferred from direct assay
(O'Farrell et al., 2017)
endosome
inferred from direct assay
(O'Farrell et al., 2017)
nucleus
inferred from direct assay
(Pan and Hardie, 2002)
Terms Based on Predictions or Assertions (3 terms)
CV Term
Evidence
References
cytoplasm
inferred from biological aspect of ancestor with PANTHER:PTN000678822
(assigned by GO_Central )
(Gaudet et al., 2011)
nucleotide-activated protein kinase complex
inferred by curator from GO:0004679
(Pan and Hardie, 2002)
nucleus
inferred from biological aspect of ancestor with PANTHER:PTN000678822
(assigned by GO_Central )
(Gaudet et al., 2011)
Gene Group (FlyBase)
Protein Family (UniProt)
-
Protein Signatures (InterPro)
Summaries
Gene Group (FlyBase)
CALCIUM/CALMODULIN-DEPENDENT PROTEIN KINASES -
The Calcium/Calmodulin-dependent kinase family are serine/threonine kinases which, in general, prefer substrates containing basic residues. Many, but not all, members are regulated by calcium or calmodulin. (Adapted from FBrf0132098).
Summary (Interactive Fly)

adenosine 5'monophosphate-activated kinase - cellular energy sensor - coordinates epithelial polarity and proliferation with cellular energy status

Gene Model and Products
Number of Transcripts
3
Number of Unique Polypeptides
1

Please see the JBrowse view of Dmel\AMPKα for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model

Gene model reviewed during 5.50

Sequence Ontology: Class of Gene
gene_with_edited_transcript
(Graveley et al., 2011)
nuclear_gene
protein_coding_gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
AMPKα-RA
FBtr0070216
NM_057965
2708
582
AMPKα-RB
FBtr0070217
NM_166880
2853
582
AMPKα-RC
FBtr0070218
NM_206604
2739
582
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
AMPKα-PA
FBpp0070208
64.6
582
8.60
NP_477313
AAF45614
AMPKα-PB
FBpp0070209
64.6
582
8.60
NP_726730
AAN09043
AMPKα-PC
FBpp0089374
64.6
582
8.60
NP_996327
AAS65245
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

582 aa isoforms: AMPKα-PA, AMPKα-PB, AMPKα-PC
Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\AMPKα using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage
adult head
(Aradska et al., 2015)
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
basolateral part of cell
inferred from direct assay
(O'Farrell et al., 2017)
endosome
inferred from direct assay
(O'Farrell et al., 2017)
nucleus
inferred from direct assay
(Pan and Hardie, 2002)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\AMPKα in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
FLIGHT - Cell culture data for RNAi and other high-throughput technologies
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyBase Wiki
Image Based Resources
Alleles, Insertions, and Transgenic Constructs
Classical and Insertion Alleles ( 12 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 24 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of AMPKα
Transgenic constructs containing regulatory region of AMPKα
Deletions and Duplications ( 2 )
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
lethal, with Scer\GAL4Mef2.PR
lethal | embryonic stage | maternal effect
lethal | P-stage
lethal | pupal stage
lethal | third instar larval stage
lethal - all die before end of larval stage
lethal - all die before end of larval stage, with Scer\GAL4αTub84B.PL
lethal - all die during P-stage, with Scer\GAL4Act.PU
lethal - all die during P-stage, with Scer\GAL4Tub.PU
lethal - all die during P-stage | recessive
partially lethal | heat sensitive, with Scer\GAL4Ubi.PU
viable, with Dcr-2UAS.cDa, Scer\GAL4elav.PLu
viable, with Scer\GAL4pnr-MD237
viable, with Scer\GAL4tin.CΔ4
Sterility
Allele
female sterile, with Scer\GAL4nos.PG, Scer\GAL4VP16.nos.UTR, Scer\GAL4VP16.otu
Other Phenotypes
Allele
cell cycle defective
cell polarity defective
cytokinesis defective
developmental rate defective | second instar larval stage
developmental rate defective | third instar larval stage
feeding behavior defective, with Scer\GAL4Ubi.PU
hyperactive | nutrition conditional, with Scer\GAL4Ubi.PU
immune response defective | adult stage, with Scer\GAL4hs.PB
immune response defective | adult stage, with Scer\GAL4NP0001
immune response defective | dominant
increased cell size | somatic clone
increased mortality | adult stage | conditional, with Scer\GAL4hs.PB
increased mortality | adult stage | conditional, with Scer\GAL4NP0001
locomotor rhythm defective | nutrition conditional, with Scer\GAL4Ubi.PU
long lived | RU486 conditional, with Scer\GAL4Mhc.Switch.PO
long lived | RU486 conditional, with Scer\GAL4Switch1.106
mitotic cell cycle defective | embryonic stage | maternal effect
mitotic cell cycle defective | larval stage
neuroanatomy defective
neuroanatomy defective, with Scer\GAL4109(2)80
neuroanatomy defective | second instar larval stage
neuroanatomy defective | third instar larval stage, with Scer\GAL4elav-C155
short lived, with Scer\GAL4hs.PB
short lived, with Scer\GAL4Ubi.PU
short lived | RU486 conditional, with Scer\GAL4Mhc.Switch.PO
short lived | RU486 conditional, with Scer\GAL4Switch1.106
size defective | adult stage | RU486 conditional, with Scer\GAL4GSG5966
small body
small body | larval stage
small body | second instar larval stage
small body | third instar larval stage
some die during larval stage
some die during larval stage, with Scer\GAL4αTub84B.PL
some die during second instar larval stage
some die during third instar larval stage
starvation stress response defective
starvation stress response defective, with AMPKαK57A.UAS, Scer\GAL4hs.PB
starvation stress response defective, with AMPKαUAS.cMa, Scer\GAL4hs.PB
starvation stress response defective, with Scer\GAL4hs.PB
starvation stress response defective, with Scer\GAL4Ubi.PU
stress response defective | dominant
visible | adult stage, with Scer\GAL4eya.enh1+E+2
Phenotype manifest in
Allele
adult midgut, with Scer\GAL4GSG5966
adult midgut, with Scer\GAL4hs.PB
adult midgut | conditional, with Scer\GAL4NP0001
autophagosome | adult stage, with Scer\GAL4hs.PB
autophagosome | adult stage | conditional, with Scer\GAL4NP0001
centrosome
circular visceral muscle fiber
dendritic arborizing neuron | second instar larval stage
denticle belt | maternal effect
embryo | maternal effect, with Scer\GAL4nos.PG, Scer\GAL4VP16.nos.UTR, Scer\GAL4VP16.otu
embryonic/first instar larval cuticle | maternal effect
embryonic/larval digestive system
embryonic/larval fat body
embryonic/larval hindgut muscle
embryonic/larval midgut
embryonic/larval midgut muscle
embryonic/larval neuromuscular junction | third instar larval stage, with Scer\GAL4elav-C155
embryonic epidermis | maternal effect
eye, with Scer\GAL4eya.enh1+E+2
follicle cell
hindgut visceral muscle fiber
larval multidendritic class IV neuron
larval multidendritic class IV neuron, with Scer\GAL4109(2)80
lipid droplet | adult stage, with Scer\GAL4hs.PB
lipid droplet | adult stage | conditional, with Scer\GAL4NP0001
lipid droplet | adult stage | RU486 conditional, with Scer\GAL4GSG5966
longitudinal visceral muscle fiber
neuroblast
NMJ bouton | third instar larval stage, with Scer\GAL4elav-C155
oenocyte
oenocyte, with Scer\GAL4Ubi.PU
sensory neuron
spindle
Orthologs
Downloads
Download All DIOPT Orthologs
Download All OrthoDB Orthologs
Human Orthologs (via DIOPT v8.0)
Homo sapiens (Human) (5)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Hsap\PRKAA2
14 of 15
Yes
Yes
Hsap\PRKAA1
11 of 15
No
Yes
2  
Hsap\BRSK1
1 of 15
No
No
Hsap\BRSK2
1 of 15
No
No
Hsap\CHEK1
1 of 15
No
No
Model Organism Orthologs (via DIOPT v8.0)
Mus musculus (laboratory mouse) (16)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Mmus\Prkaa2
14 of 15
Yes
Yes
Mmus\Prkaa1
12 of 15
No
Yes
Mmus\4921509C19Rik
1 of 15
No
Yes
Mmus\Brsk1
1 of 15
No
No
Mmus\Brsk2
1 of 15
No
No
Mmus\Gm4922
1 of 15
No
Yes
Mmus\Gm6811
1 of 15
No
Yes
Mmus\Gm7168
1 of 15
No
Yes
Mmus\Gm7356
1 of 15
No
Yes
Mmus\Gm14151
1 of 15
No
Yes
Mmus\Smok1
1 of 15
No
Yes
Mmus\Smok2a
1 of 15
No
Yes
Mmus\Smok2b
1 of 15
No
Yes
Mmus\Smok3b
1 of 15
No
Yes
Mmus\Smok3c
1 of 15
No
Yes
Mmus\Smok4a
1 of 15
No
Yes
Rattus norvegicus (Norway rat) (10)
Rnor\Prkaa2
13 of 13
Yes
Yes
Rnor\Prkaa1
11 of 13
No
Yes
Rnor\Brsk1
1 of 13
No
No
Rnor\LOC690235
1 of 13
No
Yes
Rnor\LOC100911229
1 of 13
No
Yes
Rnor\RGD1560303
1 of 13
No
Yes
Rnor\RGD1560718
1 of 13
No
Yes
Rnor\RGD1561667
1 of 13
No
Yes
Rnor\RGD1562638
1 of 13
No
Yes
Rnor\Smok2a
1 of 13
No
Yes
Xenopus tropicalis (Western clawed frog) (2)
Xtro\prkaa2
9 of 12
Yes
Yes
Xtro\prkaa1
6 of 12
No
Yes
Danio rerio (Zebrafish) (6)
Drer\prkaa1
11 of 15
Yes
Yes
Drer\prkaa2
9 of 15
No
Yes
Drer\brsk2a
1 of 15
No
No
Drer\brsk2b
1 of 15
No
No
Drer\si:ch211-255p10.4
1 of 15
No
No
Drer\si:dkey-16p21.7
1 of 15
No
No
Caenorhabditis elegans (Nematode, roundworm) (6)
Cele\aak-2
13 of 15
Yes
Yes
Cele\aak-1
7 of 15
No
Yes
Cele\C27D6.11
1 of 15
No
No
Cele\sad-1
1 of 15
No
No
Cele\tag-344
1 of 15
No
No
Cele\Y38H8A.4
1 of 15
No
No
Arabidopsis thaliana (thale-cress) (14)
Atha\KIN10
5 of 9
Yes
Yes
Atha\KIN11
5 of 9
Yes
Yes
Atha\SnRK1.3
5 of 9
Yes
Yes
Atha\AT4G11373
1 of 9
No
Yes
Atha\OST1
1 of 9
No
Yes
Atha\SNRK2-8
1 of 9
No
No
Atha\SNRK2.1
1 of 9
No
No
Atha\SNRK2.2
1 of 9
No
No
Atha\SNRK2.3
1 of 9
No
No
Atha\SNRK2.4
1 of 9
No
Yes
Atha\SNRK2.5
1 of 9
No
No
Atha\SNRK2.7
1 of 9
No
Yes
Atha\SNRK2.9
1 of 9
No
No
Atha\SNRK2.10
1 of 9
No
Yes
Saccharomyces cerevisiae (Brewer's yeast) (19)
Scer\SNF1
12 of 15
Yes
Yes
Scer\HAL5
2 of 15
No
Yes
Scer\KIN1
2 of 15
No
Yes
Scer\KIN2
2 of 15
No
No
Scer\KKQ8
2 of 15
No
Yes
Scer\NPR1
2 of 15
No
Yes
Scer\PRR2
2 of 15
No
Yes
Scer\PTK1
2 of 15
No
Yes
Scer\PTK2
2 of 15
No
Yes
Scer\FRK1
1 of 15
No
Yes
Scer\GIN4
1 of 15
No
No
Scer\HRK1
1 of 15
No
No
Scer\HSL1
1 of 15
No
No
Scer\KCC4
1 of 15
No
No
Scer\KIN4
1 of 15
No
No
Scer\RTK1
1 of 15
No
No
Scer\SAT4
1 of 15
No
No
Scer\SKS1
1 of 15
No
No
Scer\VHS1
1 of 15
No
No
Schizosaccharomyces pombe (Fission yeast) (9)
Spom\ssp2
10 of 12
Yes
Yes
Spom\cdr1
1 of 12
No
No
Spom\cdr2
1 of 12
No
No
Spom\hal4
1 of 12
No
Yes
Spom\kin1
1 of 12
No
No
Spom\oca2
1 of 12
No
Yes
Spom\ppk8
1 of 12
No
Yes
Spom\ppk9
1 of 12
No
Yes
Spom\ppk25
1 of 12
No
Yes
Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG091905MV )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila suzukii
Spotted wing Drosophila
Dsuz\DS10_00005118
Drosophila simulans
Dsim\GD16554
Drosophila sechellia
Dsec\GM19132
Drosophila erecta
Dere\GG12850
Drosophila yakuba
Dyak\GE16684
Drosophila ananassae
Dana\GF21013
Drosophila pseudoobscura pseudoobscura
Dpse\GA15892
Drosophila persimilis
Dper\GL14247
Drosophila willistoni
Dwil\GK16296
Drosophila virilis
Dvir\GJ15670
Drosophila mojavensis
Dmoj\GI16329
Drosophila grimshawi
Dgri\GH12117
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091503FL )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Mdoa\17014477
Glossina morsitans
Tsetse fly
Gmor\GMOY009836
Lucilia cuprina
Australian sheep blowfly
Lcup\FF38_04772
Mayetiola destructor
Hessian fly
Mdes\Mdes006636
Aedes aegypti
Yellow fever mosquito
Aaeg\AAEL007153
Anopheles darlingi
American malaria mosquito
Adar\ADAC002246
Anopheles gambiae
Malaria mosquito
Agam\AGAP002686
Culex quinquefasciatus
Southern house mosquito
Cqui\CPIJ002898
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W03RE )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Bmor\BGIBMGA013139
Danaus plexippus
Monarch butterfly
Dple\DPOGS212769-PA
Heliconius melpomene
Postman butterfly
Hmel\HMEL008325
Apis mellifera
Western honey bee
Amel\SNF1A
Bombus impatiens
Common eastern bumble bee
Bimp\BIMP17954
Bombus terrestris
Buff-tailed bumblebee
Bter\15209036
Linepithema humile
Argentine ant
Lhum\LH16947
Megachile rotundata
Alfalfa leafcutting bee
Mrot\MROTU:0025f7
Nasonia vitripennis
Parasitic wasp
Nvit\Nasvi2EG007650
Dendroctonus ponderosae
Mountain pine beetle
Dpod\YQE_12628
Tribolium castaneum
Red flour beetle
Tcas\Snf1a
Pediculus humanus
Human body louse
Phum\PHUM029040
Rhodnius prolixus
Kissing bug
Rpro\RPRC012957
Cimex lectularius
Bed bug
Clec\CLEC000224
Acyrthosiphon pisum
Pea aphid
Apim\ACYPI005350
Zootermopsis nevadensis
Nevada dampwood termite
Znev\Znev_09755
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X03TQ )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strigamia maritima
European centipede
Smar\SMAR013267
Ixodes scapularis
Black-legged tick
Isca\ISCW020637
Stegodyphus mimosarum
African social velvet spider
Smim\KFM77240.1
Stegodyphus mimosarum
African social velvet spider
Smim\KFM77241.1
Tetranychus urticae
Two-spotted spider mite
Turt\tetur08g07470
Tetranychus urticae
Two-spotted spider mite
Turt\tetur08g07480
Daphnia pulex
Water flea
Dpux\DAPPUDRAFT_192309
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G03TG )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Ampk
Strongylocentrotus purpuratus
Purple sea urchin
Spur\SPU_020337gn
Ciona intestinalis
Vase tunicate
Cint\LOC100184788
Gallus gallus
Domestic chicken
Ggal\PRKAA2
Gallus gallus
Domestic chicken
Ggal\ENSGALG00000014832
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v8.0)
Drosophila melanogaster (Fruit fly) (11)
Gene Symbol
Score
Source
Compara
eggNOG
Homologene
Inparanoid
Isobase
OrthoDB
OrthoFinder
orthoMCL
Panther
RoundUp
Alignment
sff
3 of 10
CG4629
2 of 10
CG8485
2 of 10
CG9222
2 of 10
CG14305
2 of 10
grp
2 of 10
Sik2
2 of 10
CG10177
1 of 10
CG17698
1 of 10
Nuak
1 of 10
Sik3
1 of 10
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Model Summary Ribbon
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 0 )
    Human Ortholog
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 5 )
    Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    PRKAA2; protein kinase AMP-activated catalytic subunit alpha 2
    14 of 15
    600497
        PRKAA1; protein kinase AMP-activated catalytic subunit alpha 1
        11 of 15
        602739
             
            Functional Complementation Data
            Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
            Interactions
            Summary of Physical Interactions
            esyN Network Diagram
            Show neighbor-neighbor interactions:
            Select Layout:
            Legend:
            Protein
            RNA
            Selected Interactor(s)
            Interactions Browser
            View in Interactions Browser
            Please see the Physical Interaction reports below for full details
            protein-protein
            Physical Interaction
            Assay
            References
            AMPKα - Lkb1
            anti tag coimmunoprecipitation, western blot
            (Dogliotti et al., 2017)
            Summary of Genetic Interactions
            esyN Network Diagram
            esyN Network Key:
            Suppression
            Enhancement
            View in Interactions Browser
            Please look at the allele data for full details of the genetic interactions
            Starting gene(s)
            Interaction type
            Interacting gene(s)
            Reference
            AMPKα
            suppressible
            ACC
            (Lee et al., 2018)
            AMPKα
            suppressible
            bmm
            (Lee et al., 2018)
            AMPKα
            suppressible
            S6k
            (Onyenwoke et al., 2015)
            AMPKα
            suppressible
            sqh
            (Bland et al., 2010, Lee et al., 2007)
            AMPKα
            suppressible
            Tor
            (Onyenwoke et al., 2015, Johnson et al., 2010)
            AMPKα
            suppressible
            Trpml
            (Onyenwoke et al., 2015)
            AMPKα
            suppressible
            Zw
            (Lee et al., 2018)
            Starting gene(s)
            Interaction type
            Interacting gene(s)
            Reference
            AdSS
            suppressible
            AMPKα
            (Stenesen et al., 2013)
            LamC
            suppressible
            AMPKα
            (Chandran et al., 2019)
            LamC
            enhanceable
            AMPKα
            (Chandran et al., 2019)
            Lkb1
            suppressible
            AMPKα
            (Lee et al., 2007)
            park
            suppressible
            AMPKα
            (Ng et al., 2012)
            Rheb
            suppressible
            AMPKα
            (Dimitroff et al., 2012)
            Rheb
            enhanceable
            AMPKα
            (Dimitroff et al., 2012)
            External Data
            Linkouts
            BioGRID - A database of protein and genetic interactions.
            DroID - A comprehensive database of gene and protein interactions.
            InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
            MIST (genetic) - An integrated Molecular Interaction Database
            MIST (protein-protein) - An integrated Molecular Interaction Database
            Pathways
            Signaling Pathways (FlyBase)
            Metabolic Pathways
            External Data
            Genomic Location and Detailed Mapping Data
            Chromosome (arm)
            X
            Recombination map

            1-0.2

            Cytogenetic map
            2A1-2A1
            Sequence location
            X:1,375,536..1,378,896 [+]
            FlyBase Computed Cytological Location
            Cytogenetic map
            Evidence for location
            2A1-2A1
            Limits computationally determined from genome sequence between P{EP}EP1542 and P{EP}futschEP1419
            Experimentally Determined Cytological Location
            Cytogenetic map
            Notes
            References
            Experimentally Determined Recombination Data
            Location

            1-0.2

            (FlyBase, 2016)

            1-0.1

            (Comeron et al., 2012)
            Left of (cM)
            Right of (cM)
            Notes
            Stocks and Reagents
            Stocks (25)
            Genomic Clones (12)
             

            Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

            cDNA Clones (104)
             

            Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

            cDNA clones, fully sequenced
            BDGP DGC clones
            Other clones
            Drosophila Genomics Resource Center cDNA clones

            For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

            cDNA Clones, End Sequenced (ESTs)
            BDGP DGC clones
            Other clones
            RNAi and Array Information
            Linkouts
            DRSC - Results frm RNAi screens
            GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
            Antibody Information
            Laboratory Generated Antibodies
             

            polyclonal

            (Bland et al., 2010)
            Commercially Available Antibodies
             
            Cell Signaling Technology - Commercial vendor for primary antibodies and antibody conjugates.
            Other Information
            Relationship to Other Genes
            Source for database identify of

            Source for identity of: AMPKα SNF1A

            (FlyBase, 2009-)
            Source for database merge of

            Source for merge of: SNF1A CG3051

            (Bayraktaroglu, 2000.4.12)

            Source for merge of: SNF1A Gprk4

            (Misra, 2000.7.28)
            Additional comments

            Changed symbol from 'SNF1A' to 'AMPKα' to reflect overwhelming usage in the published literature, including all research papers to characterize the gene.

            (FlyBase, 2009-)
            Other Comments

            Reduced SNF1A signaling leads to hypersensitivity to starvation conditions as measured by lifespan and locomotor activity.

            (Johnson et al., 2010)

            Reduced SNF1A function results in hyperphagia.

            (Johnson et al., 2010)

            S2 cells treated with dsRNA generated against this gene show reduced phagocytosis of Candida albicans compared to untreated cells.

            (Stroschein-Stevenson et al., 2006)

            When dsRNA constructs are made and transiently transfected into S2 cells in RNAi experiments, an increase in the proportion of S phase cells, an increase in the proportion of G2/M phase cells, a whole range of mitotic abnormalities, and centrosome abnormalities are seen.

            (Bettencourt-Dias et al., 2004)
            Origin and Etymology
            Discoverer
            Etymology
            Identification
            External Crossreferences and Linkouts ( 67 )
            Sequence Crossreferences
            NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
            GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
            GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
            RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
            UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
            Other crossreferences
            BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
            Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
            Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
            Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
            Flygut - An atlas of the Drosophila adult midgut
            GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
            iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
            InterPro - A database of protein families, domains and functional sites
            KEGG Genes - Molecular building blocks of life in the genomic space.
            modMine - A data warehouse for the modENCODE project
            SignaLink - A signaling pathway resource with multi-layered regulatory networks.
            Linkouts
            BioGRID - A database of protein and genetic interactions.
            Cell Signaling Technology - Commercial vendor for primary antibodies and antibody conjugates.
            Cell Signaling Technology - Commercial vendor for primary antibodies and antibody conjugates.
            DroID - A comprehensive database of gene and protein interactions.
            DRSC - Results frm RNAi screens
            FLIGHT - Cell culture data for RNAi and other high-throughput technologies
            FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
            FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
            FlyMine - An integrated database for Drosophila genomics
            Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
            InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
            KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.
            MIST (genetic) - An integrated Molecular Interaction Database
            MIST (protein-protein) - An integrated Molecular Interaction Database
            Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
            Synonyms and Secondary IDs (32)
            Reported As
            Symbol Synonym
            AK
            (Ng et al., 2012)
            AMPK
            (Bednářová et al., 2018, Lee et al., 2018, Staats et al., 2018, Dogliotti et al., 2017, Evans et al., 2017, Galasso et al., 2017, Ng et al., 2017, Richardson and Portela, 2017, Lee et al., 2016, Li et al., 2016, Wang et al., 2016, Kim and Lee, 2015, Yang et al., 2015, Gündner et al., 2014, Lin et al., 2014, Ulgherait et al., 2014, Wang et al., 2014, Yin et al., 2014, Di Cara et al., 2013, Hur et al., 2013, Kim et al., 2013, Owusu-Ansah et al., 2013, Stenesen et al., 2013, Tixier et al., 2013, Tixier et al., 2013, Dimitroff et al., 2012, Ng et al., 2012, Onyenwoke et al., 2012, Slack et al., 2012, Wang et al., 2012, Wang et al., 2011, Lee et al., 2010, Mirouse et al., 2009, Mirouse et al., 2009, Brenman et al., 2008, Hoshizaki and Gibbs, 2007, Lee et al., 2007, Libert, 2007, Spasic et al., 2007, Wang et al., 2007, Edgar, 2006, McBride et al., 2006, Pan et al., 2004)
            AMPK alpha
            (Lee et al., 2019)
            AMPK-α
            (Sopko et al., 2015)
            AMPKalpha
            (Molaei et al., 2019, Zheng, 2019.3.1, Transgenic RNAi Project members, 2017-, Haelterman, 2015.3.20, Sopko et al., 2014, Yamamoto et al., 2013-, Frei et al., 2007)
            AMPKα
            (Chittoor-Vinod et al., 2020, Graves et al., 2020, Kierdorf et al., 2020, Yuan et al., 2020, Chandran et al., 2019, Cho et al., 2019, Drummond-Barbosa, 2019, Evangelakou et al., 2019, Hegazi et al., 2019, Maier et al., 2019, Su et al., 2019, van den Ameele and Brand, 2019, Yurgel et al., 2019, Nagy et al., 2018, Galasso et al., 2017, Dobson et al., 2016, Laws and Drummond-Barbosa, 2016, Kanoh et al., 2015, Radermacher et al., 2014, Swick et al., 2013, Andersen et al., 2012, Braco et al., 2012, Pimenta de Castro et al., 2012, Choi et al., 2011, Johnson et al., 2010, Kazgan et al., 2010, Moser et al., 2010)
            AmpKα
            (Pimenta de Castro et al., 2012)
            CG3051
            (Yurgel et al., 2019, Hosono et al., 2015, Schertel et al., 2013, Swick et al., 2013, Christis and Munro, 2012, Choi et al., 2011, Ni et al., 2011.7.19, Bland et al., 2010, Ni et al., 2010.12.1, Sun et al., 2010, Tohyama and Yamaguchi, 2010, Zhang et al., 2010, Keleman et al., 2009.8.5, Perkins et al., 2009.1.15, Benos, 2000.12.13, Morrison et al., 2000)
            DmAMPK α
            (Pan and Hardie, 2002)
            EG:132E8.2
            (Benos et al., 2001, Benos, 2000.12.13)
            FBgn0023169
            (Pan and Hardie, 2002)
            Gprk-4
            (Cassill et al., 1991)
            Gprk4
            SNF1A
            (Tsuyama et al., 2017, Brewer-Jensen et al., 2016, Izutsu et al., 2015, Onyenwoke et al., 2015, Ugrankar et al., 2015, Nie et al., 2014, Pogson et al., 2014, Sopko et al., 2014, Lu and Johnston, 2013, Schertel et al., 2013, Japanese National Institute of Genetics, 2012.5.21, Papatheodorou et al., 2012, Lindquist et al., 2011, Brenman, 2010.9.17, Fernández-Ayala et al., 2010, Moser et al., 2010, Sun et al., 2010, Zhou et al., 2008, Stroschein-Stevenson et al., 2006)
            SNF4A-a
            (Sopko et al., 2015)
            ampk
            (Gailite et al., 2015, Wong et al., 2015, Amin et al., 2009)
            ampkα
            (Haack et al., 2013, Chakrabarti et al., 2012, Amin et al., 2009)
            dAMPK
            (Ng et al., 2012)
            dAMPKa
            (Bland et al., 2007)
            dAMPKα
            (Bland et al., 2010, Tohyama and Yamaguchi, 2010)
            snf1A
            (Braco et al., 2012)
            snf1a
            (Andolfatto and Wall, 2003)
            Name Synonyms
            AMP activated kinase
            (Di Cara et al., 2013)
            AMP-activated kinase
            (Wang et al., 2012)
            AMP-activated protein kinase
            (Laws and Drummond-Barbosa, 2016, Kim and Lee, 2015, Gündner et al., 2014, Owusu-Ansah et al., 2013, Braco et al., 2012, Onyenwoke et al., 2012, Mirouse et al., 2009, Lee et al., 2007, Spasic et al., 2007, Baehrecke, 2006)
            AMP-activated protein kinase α subunit
            (Graves et al., 2020)
            AMP-dependent kinase
            (Edgar, 2006)
            AMPK alpha subunit
            (Piper and Partridge, 2018)
            AMPK catalytic subunit
            (Swick et al., 2013)
            G protein-coupled receptor kinase 4
            SNF1A/AMP-activated protein kinase
            adenosine monophosphate-activated protein kinase
            (Lee et al., 2010)
            Secondary FlyBase IDs
            • FBgn0004836
            • FBgn0029574
            Datasets (0)
            Study focus (0)
            Experimental Role
            Project
            Project Type
            Title
            References (174)