DDT-R, cytochrome P450, Rst(2)DDT, 6g1
Gene model reviewed during 5.49
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Cyp6g1 using the Feature Mapper tool.
GBrowse - Visual display of RNA-Seq signalsView Dmel\Cyp6g1 in GBrowse 2
2-56.2 is the map position of DDT resistance in the 91R strain, assuming a single locus is involved. The 95% confidence interval is 2-55.1 to 2-56.7.
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
FlyBase curator comment: the relationship between the "Rst(2)DDT-WIS1", "Cyp6g1", "Rst(2)DDT-EMS1" and "Rst(2)DDT-EMS2" genes is unclear. The "Rst(2)DDT-WIS1WIS1", "Cyp6g1DDT-R", "Rst(2)DDT-EMS1EMS1" and "Rst(2)DDT-EMS2EMS2" DDT resistant strains all map to the same region of the genome, which contains a cluster of P450 genes, but it has not been established whether or not they represent lesions in the same gene.
Source for merge of Cyp6g1 anon-WO03025223.16 anon-WO03025223.17 was sequence comparison ( date:051113 ).
Possible to select new resistant alleles by growing the offspring of irradiated wild-type flies on parathion (Kikkawa, 1968). DDT resistance in the resistant 91R population mapped by Dapkus (1992) to 2-56.2 (95% confidence limit, 2-55.1 to 2-56.2), assuming a single locus. In other studies, with other strains, DDT resistance shown to be due to more than one locus (Crow 1954; Crow, 1957; King and Somme, 1958; Dapkus and Merrell, 1977). The relationship between Rst(2)DDT and Cyp6a2 is unclear.
Overtranscription of the Cyp6g1 gene alone is necessary and sufficient for resistance to DDT.
GH03082 is a chimeric cDNA clone containing two cDNAs in a tail-to-tail orientation that correspond to hoip (5' clone, mapped at 30C) and Cyp6g1 (GenBank accession number AF083946; 3' clone, mapped at 48F).
Tsukamoto and Ogaki, 1953.