FlyBase Gene Report: Dmel\Tip60

General Information

Symbol

Dmel\Tip60

Species

D. melanogaster

Name

Tat interactive protein 60kDa

Annotation Symbol

CG6121

Feature Type

protein_coding_gene

FlyBase ID

FBgn0026080

Gene Model Status

Current

Stock Availability

10 publicly available

Gene Snapshot

Tat interactive protein 60kDa (Tip60) encodes a lysine acetyltransferase that acetylates histone proteins to regulate chromatin packaging and epigenetic gene control. It also acetylates non-histone proteins, and plays a role in apoptosis, DNA repair and various neural processes. [Date last reviewed: 2019-03-14]

Other Summaries

Alliance
Auto summary
Gene Group
UniProtKB
Interactive Fly

Also Known As

dTip60, EG0007.7, EG:EG0007.7 , Dmel/TIP60

Key Links

Genomic Location

Cytogenetic map

4A6-4B1

Sequence location

X:4,114,800..4,117,487 [-]

Recombination map

1-6

RefSeq locus

NC_004354 REGION:4114800..4117487

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse GBrowse

Help me choose

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

Gene Ontology (GO) Annotations (19 terms)

Molecular Function (4 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

enables euchromatin binding

inferred from direct assay

(Panikker et al., 2018)

enables histone acetyltransferase activity

inferred from mutant phenotype

(Lorbeck et al., 2011)

inferred from direct assay

(assigned by UniProt )

(Kusch et al., 2004)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

enables histone acetyltransferase activity

inferred from biological aspect of ancestor with PANTHER:PTN004172615

(assigned by GO_Central )

(Gaudet et al., 2011)

enables histone binding

inferred from biological aspect of ancestor with PANTHER:PTN000064894

(assigned by GO_Central )

(Gaudet et al., 2011)

enables transcription coregulator activity

inferred from biological aspect of ancestor with PANTHER:PTN000064894

(assigned by GO_Central )

(Gaudet et al., 2011)

Biological Process (11 terms)

Terms Based on Experimental Evidence (9 terms)

CV Term

Evidence

References

involved_in histone acetylation

inferred from direct assay

(Kusch et al., 2004)

inferred from mutant phenotype

(Scacchetti et al., 2020)

involved_in histone exchange

inferred from direct assay

(Kusch et al., 2004)

involved_in histone H4 acetylation

inferred from mutant phenotype

(Lorbeck et al., 2011)

involved_in negative regulation of neuron apoptotic process

inferred from mutant phenotype

(Pirooznia et al., 2012)

involved_in nervous system development

inferred from mutant phenotype

(Lorbeck et al., 2011)

involved_in regulation of gene expression

inferred from mutant phenotype

(Pirooznia et al., 2012)

involved_in regulation of synaptic plasticity

inferred from direct assay

(Sarthi and Elefant, 2011)

involved_in regulation of terminal button organization

inferred from direct assay

(Sarthi and Elefant, 2011)

involved_in regulation of transcription, DNA-templated

inferred from mutant phenotype

(Scacchetti et al., 2020)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

involved_in negative regulation of transcription, DNA-templated

inferred from biological aspect of ancestor with PANTHER:PTN000064894

(assigned by GO_Central )

(Gaudet et al., 2011)

involved_in positive regulation of transcription by RNA polymerase II

inferred from biological aspect of ancestor with PANTHER:PTN000064894

(assigned by GO_Central )

(Gaudet et al., 2011)

Cellular Component (4 terms)

Terms Based on Experimental Evidence (4 terms)

CV Term

Evidence

References

part_of histone acetyltransferase complex

inferred from physical interaction with FLYBASE:pont; FB:FBgn0040078

(Kusch et al., 2004)

part_of NuA4 histone acetyltransferase complex

inferred from direct assay

(Kusch et al., 2004)

located_in nucleus

inferred from direct assay

(Panikker et al., 2018, Schirling et al., 2010, Kusch et al., 2004)

located_in polytene chromosome interband

inferred from direct assay

(Schirling et al., 2010)

Terms Based on Predictions or Assertions (0 terms)

Gene Group (FlyBase)

TIP60 COMPLEX

HISTONE ACETYLTRANSFERASES

Protein Family (UniProt)

Belongs to the MYST (SAS/MOZ) family. (Q960X4)

Protein Signatures (InterPro)

Summaries

Gene Group (FlyBase)

TIP60 COMPLEX -: The Tip60 chromatin remodeling complex acetylates nucleosomal phospho-histone H2Av and exchanges it with unmodified H2Av. (Adapted from FBrf0185742).
HISTONE ACETYLTRANSFERASES -: Histone acetyltransferases catalyze the acetylation of conserved lysine residues on histone proteins by transferring an acetyl group from acetyl CoA to form ε-N-acetyl lysine. (Adapted from PMID:11395403).

Protein Function (UniProtKB)

Catalytic subunit of the Tip60 chromatin-remodeling complex which is involved in DNA repair. Upon induction of DNA double-strand breaks, acetylates phosphorylated histone H2AV in nucleosomes on 'Lys-4' and exchanges it with unmodified H2AV.

(UniProt, Q960X4)

Summary (Interactive Fly)

histone acetyltransferase that regulates general metabolism, axon growth and dendritic targeting and sleep-wake cycle - regulates acetylation of histone H2A and promotes the generation of silent chromatin

Interactive Fly

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Tip60 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Comments on Gene Model

Stop Codon spans the intron.

Gene model reviewed during 5.46

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Tip60-RA

FBtr0070668

NM_131923

2390

541

Tip60-RB

FBtr0333185

NM_001272304

2368

473

Tip60-RC

FBtr0333186

NM_001272305

1852

541

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

Tip60-PA

FBpp0070636

61.2

541

8.77

NP_572151

AAF45923

Tip60-PB

FBpp0305387

53.0

473

8.39

NP_001259233

AGB95079

Tip60-PC

FBpp0305388

61.2

541

8.77

NP_001259234

AGB95080

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

541 aa isoforms: Tip60-PA, Tip60-PC

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Subunit Structure (UniProtKB)

Component of the Tip60 chromatin-remodeling complex which contains the catalytic subunit Tip60 and the subunits Domino, Tra1, Brd8, E(Pc), DMAP1, Pontin, Reptin, Ing3, Act87E, BAP55, Mrg15, MrgBP, Gas41 and YL-1.

(UniProt, Q960X4)

Post Translational Modification

Autoacetylation at Lys-355 is required for proper function.

(UniProt, Q960X4)

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Tip60 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Linkouts

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

third instar larval stage

NMJ bouton

(Sarthi and Elefant, 2011)

embryonic/larval neuromuscular junction

(Sarthi and Elefant, 2011)

Additional Descriptive Data

Tip60 is localized at both the pre and postsynaptic sides of the neuromuscular junction and in presynaptic boutons.

(Sarthi and Elefant, 2011)

Marker for

Subcellular Localization

CV Term

Evidence

References

part_of histone acetyltransferase complex

inferred from physical interaction with FLYBASE:pont; FB:FBgn0040078

(Kusch et al., 2004)

part_of NuA4 histone acetyltransferase complex

inferred from direct assay

(Kusch et al., 2004)

located_in nucleus

inferred from direct assay

(Panikker et al., 2018, Schirling et al., 2010, Kusch et al., 2004)

located_in polytene chromosome interband

inferred from direct assay

(Schirling et al., 2010)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Tip60 in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Tip60

View exonic expression by tissue for Dmel\Tip60

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0026080

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG6121-RA

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG6121

Flygut - An atlas of the Drosophila adult midgut

FBgn0026080

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 3 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 16 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Tip60

Transgenic constructs containing regulatory region of Tip60

Aberrations (Deficiencies and Duplications) ( 0 )

Inferred from experimentation

Inferred from location

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal, with Scer\GAL4^179Y

Tip60^E431Q.UAS

lethal, with Scer\GAL4^337Y

lethal, with Scer\GAL4^da.G32

Tip60^{dsRNA.UAS.cSa}

lethal | heat sensitive, with Scer\GAL4^T80

Tip60^{dsRNA.UAS.cSa}

lethal | pupal stage, with Scer\GAL4^pnr-MD237

Tip60^GD11826

lethal - all die before end of prepupal stage, with Scer\GAL4^69B

Tip60^GD11826

lethal - all die before end of pupal stage, with Scer\GAL4^337Y

Tip60^E431Q.UAS

lethal - all die before end of pupal stage, with Scer\GAL4^Act5C.PI

lethal - all die before end of pupal stage, with Scer\GAL4^elav-C155

lethal - all die before end of pupal stage, with Scer\GAL4^Mef2.247

partially lethal, with Scer\GAL4^how-24B

Tip60^dsRNA.Z.UAS

partially lethal - majority die, with Scer\GAL4^60IIA

Tip60^E431Q.UAS

partially lethal - majority die, with Scer\GAL4^179Y

partially lethal - majority die, with Scer\GAL4^337Y

Tip60^UAS.cPa

partially lethal - majority die, with Scer\GAL4^elav.PLu

Tip60^E431Q.UAS

partially lethal - majority die | pupal stage, with Scer\GAL4^pnr-MD237

Tip60^GD11826

viable

Tip60^e02395

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Tip60^GD11826

viable, with Scer\GAL4^337Y

Tip60^UAS.cLa

viable, with Scer\GAL4^Act5C.PI

viable, with Scer\GAL4^en-e16E

Tip60^{dsRNA.UAS.cSa}

viable, with Scer\GAL4^tin.CΔ4

Tip60^GD11826

Sterility

Allele

female semi-fertile, with Scer\GAL4^{VP16.mat.αTub67C}

female semi-sterile, with Scer\GAL4^nos.PG, Scer\GAL4^VP16.nos.UTR, Scer\GAL4^VP16.otu

fertile

Tip60^e02395

Other Phenotypes

Allele

abnormal locomotor behavior | third instar larval stage, with Scer\GAL4^elav-C155

abnormal memory | adult stage, with Scer\GAL4^ey-OK107

abnormal neuroanatomy | adult stage, with Scer\GAL4^ey-OK107

abnormal neuroanatomy | adult stage, with Scer\GAL4^P2.4.Pdf

abnormal neuroanatomy | larval stage, with Scer\GAL4^elav-C155

abnormal neuroanatomy | larval stage, with Scer\GAL4^Mef2.247

abnormal sleep | adult stage, with Scer\GAL4^P2.4.Pdf

flightless, with Scer\GAL4^Mef2.PR

Tip60^GD11826

increased cell death, with Scer\GAL4^en-e16E

Tip60^{dsRNA.UAS.cSa}

increased cell death | third instar larval stage, with Scer\GAL4^179Y

Tip60^E431Q.UAS

increased cell number | spermatogenesis, with Scer\GAL4^NP1624

increased mortality during development, with Scer\GAL4^179Y

Tip60^E431Q.UAS

increased mortality during development, with Scer\GAL4^337Y

Tip60^E431Q.UAS

majority die during third instar larval stage, with Scer\GAL4^179Y

Tip60^E431Q.UAS

majority die during third instar larval stage, with Scer\GAL4^337Y

Tip60^E431Q.UAS

neoplasia | spermatogenesis, with Scer\GAL4^NP1624

some die during pupal stage, with Scer\GAL4^337Y

Tip60^E431Q.UAS

some die during pupal stage, with Scer\GAL4^Act5C.PI

some die during pupal stage, with Scer\GAL4^elav-C155

some die during pupal stage, with Scer\GAL4^Mef2.247

visible, with Scer\GAL4^en-e16E

Tip60^{dsRNA.UAS.cSa}

visible, with Scer\GAL4^pnr-MD237

Tip60^GD11826

visible, with Scer\GAL4^T80

Tip60^{dsRNA.UAS.cSa}

Phenotype manifest in

Allele

adult mushroom body, with Scer\GAL4^ey-OK107

adult mushroom body alpha'-lobe, with Scer\GAL4^ey-OK107

Tip60^E431Q.UAS

adult mushroom body alpha-lobe, with Scer\GAL4^ey-OK107

Tip60^E431Q.UAS

adult mushroom body beta'-lobe, with Scer\GAL4^ey-OK107

Tip60^E431Q.UAS

adult mushroom body beta-lobe, with Scer\GAL4^ey-OK107

Tip60^E431Q.UAS

adult mushroom body gamma-lobe, with Scer\GAL4^ey-OK107

Tip60^E431Q.UAS

axon | adult stage, with Scer\GAL4^P2.4.Pdf

cyst progenitor cell | increased number, with Scer\GAL4^NP1624

egg, with Scer\GAL4^{VP16.mat.αTub67C}

egg chamber, with Scer\GAL4^nos.PG, Scer\GAL4^VP16.nos.UTR, Scer\GAL4^VP16.otu

Tip60^HM05049

egg chamber, with Scer\GAL4^{VP16.mat.αTub67C}

embryonic/larval brain | third instar larval stage, with Scer\GAL4^179Y

Tip60^E431Q.UAS

indirect flight muscle, with Scer\GAL4^Mef2.PR

Tip60^GD11826

male germline cell | increased number, with Scer\GAL4^NP1624

male germline stem cell | increased number, with Scer\GAL4^NP1624

myofibril, with Scer\GAL4^Mef2.PR

Tip60^GD11826

nurse cell, with Scer\GAL4^{VP16.mat.αTub67C}

ovary | adult stage, with Scer\GAL4^nos.PG, Scer\GAL4^VP16.nos.UTR, Scer\GAL4^VP16.otu

Tip60^HM05049

sarcomere, with Scer\GAL4^Mef2.PR

Tip60^GD11826

s-LNv Pdf neuron | adult stage, with Scer\GAL4^P2.4.Pdf

spermatogonial cyst, with Scer\GAL4^NP1624

terminal bouton, with Scer\GAL4^elav-C155

terminal bouton, with Scer\GAL4^Mef2.247

trichogen cell, with Scer\GAL4^pnr-MD237

Tip60^GD11826

type I bouton | ectopic, with Scer\GAL4^elav-C155

type I bouton | ectopic, with Scer\GAL4^Mef2.247

type Ib terminal bouton | ectopic, with Scer\GAL4^elav-C155

type Ib terminal bouton | ectopic, with Scer\GAL4^Mef2.247

type Is terminal bouton, with Scer\GAL4^Mef2.247

wing, with Scer\GAL4^en-e16E

Tip60^{dsRNA.UAS.cSa}

wing, with Scer\GAL4^T80

Tip60^{dsRNA.UAS.cSa}

wing disc, with Scer\GAL4^en-e16E

Tip60^{dsRNA.UAS.cSa}

wing vein | ectopic, with Scer\GAL4^en-e16E

Tip60^{dsRNA.UAS.cSa}

wing vein | ectopic, with Scer\GAL4^T80

Tip60^{dsRNA.UAS.cSa}

Z disc, with Scer\GAL4^Mef2.PR

Tip60^GD11826

Orthologs

Downloads

Download All DIOPT Orthologs

Download All OrthoDB Orthologs

Human Orthologs (via DIOPT v8.0)

Homo sapiens (Human) (6)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Hsap\KAT5

13 of 15

Yes

5 of 15

3 of 15

2 of 15

2 of 15

1 of 15

Model Organism Orthologs (via DIOPT v8.0)

Mus musculus (laboratory mouse) (6)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Mmus\Kat5

14 of 15

Yes

4 of 15

3 of 15

2 of 15

2 of 15

1 of 15

Rattus norvegicus (Norway rat) (5)

Rnor\Kat5

9 of 13

Yes

4 of 13

1 of 13

1 of 13

1 of 13

Xenopus tropicalis (Western clawed frog) (6)

Xtro\kat5

8 of 12

Yes

Xtro\kat8

4 of 12

Xtro\kat7

2 of 12

Xtro\kat6a

1 of 12

Yes

Xtro\trim69.1

1 of 12

Yes

Xtro\xnf7

1 of 12

Yes

Danio rerio (Zebrafish) (8)

Drer\kat5a

14 of 15

Yes

Drer\kat5b

14 of 15

Yes

4 of 15

3 of 15

2 of 15

2 of 15

1 of 15

1 of 15

Caenorhabditis elegans (Nematode, roundworm) (5)

Cele\mys-1

11 of 15

Yes

5 of 15

4 of 15

3 of 15

1 of 15

Yes

Arabidopsis thaliana (thale-cress) (2)

Atha\HAM1

5 of 9

Yes

Atha\HAM2

5 of 9

Yes

Saccharomyces cerevisiae (Brewer's yeast) (3)

Scer\ESA1

11 of 15

Yes

Scer\SAS3

5 of 15

Yes

Scer\SAS2

3 of 15

Schizosaccharomyces pombe (Fission yeast) (2)

Spom\mst1

10 of 12

Yes

Spom\mst2

3 of 12

Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG091907LG )

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila suzukii

Spotted wing Drosophila

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA25056

Drosophila pseudoobscura pseudoobscura

Dpse\GA19369

Drosophila persimilis

Dper\GL21384

Drosophila persimilis

Dper\GL14311

Drosophila persimilis

Dper\GL18118

Drosophila willistoni

Dwil\GK23796

Drosophila willistoni

Dwil\GK17633

Drosophila virilis

Dvir\GJ16425

Drosophila virilis

Dvir\GJ13223

Drosophila mojavensis

Dmoj\GI20630

Drosophila mojavensis

Dmoj\GI16149

Drosophila grimshawi

Dgri\GH24817

Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091503KM )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Musca domestica

House fly

Mdoa\17015179

Glossina morsitans

Tsetse fly

Gmor\GMOY010149

Mayetiola destructor

Hessian fly

Mdes\Mdes010651

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL014072

Anopheles darlingi

American malaria mosquito

Adar\ADAC009917

Anopheles gambiae

Malaria mosquito

Agam\AGAP001539

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ001206

Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W0477 )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Bombyx mori

Silkmoth

Bmor\BGIBMGA008516

Danaus plexippus

Monarch butterfly

Dple\DPOGS206682-PA

Heliconius melpomene

Postman butterfly

Hmel\HMEL006569

Apis florea

Little honeybee

Aflo\3429924596

Apis mellifera

Western honey bee

Amel\Tip60

Bombus impatiens

Common eastern bumble bee

Bimp\BIMP14192

Bombus terrestris

Buff-tailed bumblebee

Bter\15213639

Linepithema humile

Argentine ant

Lhum\LH22423

Megachile rotundata

Alfalfa leafcutting bee

Mrot\MROTU:00193e

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG000377

Dendroctonus ponderosae

Mountain pine beetle

Dpod\YQE_03759

Dendroctonus ponderosae

Mountain pine beetle

Dpod\YQE_10390

Tribolium castaneum

Red flour beetle

Tcas\GLEAN_08142

Pediculus humanus

Human body louse

Phum\PHUM229890

Rhodnius prolixus

Kissing bug

Rpro\RPRC012392

Cimex lectularius

Bed bug

Clec\CLEC002716

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI007943

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_00128

Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X043R )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strigamia maritima

European centipede

Smar\SMAR009060

Ixodes scapularis

Black-legged tick

Isca\ISCW020584

Stegodyphus mimosarum

African social velvet spider

Smim\KFM57827.1

Stegodyphus mimosarum

African social velvet spider

Smim\KFM57828.1

Tetranychus urticae

Two-spotted spider mite

Turt\tetur22g00860

Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G0B73 )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Myst2

Strongylocentrotus purpuratus

Purple sea urchin

Spur\SPU_028153gn

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Kat5

Ciona intestinalis

Vase tunicate

Cint\LOC100177949

Ciona intestinalis

Vase tunicate

Cint\ENSCING00000024909

Ciona intestinalis

Vase tunicate

Cint\LOC100179857

Gallus gallus

Domestic chicken

Ggal\ENSGALG00000029060

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v8.0)

Drosophila melanogaster (Fruit fly) (8)

Gene Symbol

Score

Source

Alignment

mof

7 of 10

chm

5 of 10

enok

5 of 10

CG1894

4 of 10

2 of 10

e(y)3

2 of 10

tth

2 of 10

CG8677

1 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 3 )

Allele

Disease

Interaction

References

Tip60^UAS.cLa

ameliorates Alzheimer's disease

modeled by Hsap\APP^{695.UAS.Tag:MYC}

(Xu et al., 2016, Xu et al., 2014, Johnson et al., 2013, Pirooznia et al., 2012)

Tip60^UAS.cPa

ameliorates Alzheimer's disease

modeled by Hsap\APP^{695.UAS.Tag:MYC}

(Panikker et al., 2018, Pirooznia et al., 2012)

Tip60^E431Q.UAS

exacerbates Alzheimer's disease

modeled by Hsap\APP^{695.UAS.Tag:MYC}

(Xu et al., 2016, Johnson et al., 2013)

Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

KAT5; lysine acetyltransferase 5

13 of 15

601409

NEURODEVELOPMENTAL DISORDER WITH DYSMORPHIC FACIES, SLEEP DISTURBANCE, AND BRAIN ABNORMALITIES; NEDFASB

KAT8; lysine acetyltransferase 8

5 of 15

609912

LI-GHORBANI-WEISZ-HUBSHMAN SYNDROME; LIGOWS

KAT7; lysine acetyltransferase 7

3 of 15

609880

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

View in Interactions Browser

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Tip60 - Bacc

anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based

(Rhee et al., 2014)

Tip60 - Blos1

two hybrid array

(Shokri et al., 2019)

Tip60 - CG8677

anti bait coimmunoprecipitation, western blot

(Hanai et al., 2008)

Tip60 - Dp

anti tag coimmunoprecipitation, western blot

(Flegel et al., 2016)

Tip60 - E(Pc)

anti bait coimmunoprecipitation, western blot

(Kusch et al., 2004)

Tip60 - Gas41

anti bait coimmunoprecipitation, western blot

(Kusch et al., 2004)

Tip60 - His2A

enzymatic study, autoradiography

(Kusch et al., 2014)

Tip60 - His2Av

enzymatic study, western blot, anti bait coimmunoprecipitation

(Kusch et al., 2014, Kusch et al., 2004)

Tip60 - His2B

anti bait coimmunoprecipitation, western blot

(Kusch et al., 2004)

Tip60 - His4

enzymatic study, autoradiography

(Kusch et al., 2014)

Tip60 - Hsf

anti bait coimmunoprecipitation, western blot

(Kusch et al., 2014)

Tip60 - Ing3

anti bait coimmunoprecipitation, western blot

(Kusch et al., 2004)

Tip60 - MRG15

anti bait coimmunoprecipitation, western blot

(Kusch et al., 2004)

Tip60 - Nipped-A

anti bait coimmunoprecipitation, western blot

(Kusch et al., 2004)

Tip60 - Ubx

bimolecular fluorescence complementation, fluorescence microscopy

(Bischof et al., 2018)

Tip60 - dom

anti tag coimmunoprecipitation, Identification by mass spectrometry, anti bait coimmunoprecipitation, western blot

(Scacchetti et al., 2020, Kusch et al., 2004)

Tip60 - pont

anti tag coimmunoprecipitation, peptide massfingerprinting, anti bait coimmunoprecipitation, anti tag western blot, western blot

(Kusch et al., 2004)

Tip60 - rept

anti bait coimmunoprecipitation, anti tag western blot

(Kusch et al., 2004)

Summary of Genetic Interactions

esyN Network Diagram

esyN Network Key:

Suppression

Enhancement

View in Interactions Browser

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Tip60

enhanceable

E(Pc)

(Feng et al., 2017)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

dom

enhanceable

Tip60

(Kwon et al., 2013)

E(Pc)

enhanceable

Tip60

(Feng et al., 2017)

External Data

Subunit Structure (UniProtKB)

(UniProt, Q960X4 )

Linkouts

BioGRID - A database of protein and genetic interactions.

57879

DroID - A comprehensive database of gene and protein interactions.

FBgn0026080

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

31362

MIST (genetic) - An integrated Molecular Interaction Database

Tip60

MIST (protein-protein) - An integrated Molecular Interaction Database

Tip60

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

External Data

Linkouts

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

1-6

Cytogenetic map

4A6-4B1

Sequence location

X:4,114,800..4,117,487 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

4A6-4B1

Limits computationally determined from genome sequence between P{EP}CG2930^EP1352 and P{EP}EP425

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

1-6

(FlyBase, 2016)

1-6.0

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (10)

Bloomington

18052

w¹¹¹⁸ PBac{RB}Tip60^e02395

28563

y¹ v¹; P{TRiP.HM05049}attP2

15630

y¹ w^67c23 P{Mae-UAS.6.11}Tip60^GG01739

35243

y¹ sc^* v¹ sev²¹; P{TRiP.GL00130}attP2

79937

y¹ sc^* v¹ sev²¹; P{TOE.GS02223}attP40

91561

y^* w¹¹¹⁸ Tip60⁷⁰⁴ P{ey-FLP.N}2 P{neoFRT}19A/FM0; P{PRE.Scr-PS1}Q1

FlyORF

F003462

M{UAS-Tip60.ORF-CC}ZH-21F

F000567

M{UAS-Tip60.ORF.3xHA.GW}ZH-86Fb

VDRC

v110617

P{KK107642}VIE-260B

v22233

w¹¹¹⁸ P{GD11826}v22233

Genomic Clones (13)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (52)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

LD31064

Other clones

DPiM_LD31064

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0026080

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0026080

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

31362

Antibody Information

Laboratory Generated Antibodies

monoclonal

(Scacchetti et al., 2020)

polyclonal

(Schirling et al., 2010, Kusch et al., 2004)

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for identity of: EG:EG0007.7 CG6121

(Zhao, 1999.11)

Source for identity of: Tip60 CG6121

(Kusch et al., 2004)

Source for database merge of

Additional comments

Other Comments

Tip60 controls neuronal specific gene expression profiles that are required for appropriate development and function of the nervous system.

(Lorbeck et al., 2011)

DNA-protein interactions: genome-wide binding profile assayed for Tip60 protein in Kc167 cells; see Chromatin_types_NKI collection report. Individual protein-binding experiments listed under "Samples" at GEO_GSE22069 (http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE22069).

(Filion et al., 2010)

Identified in an RNAi screen for host factors that alter infection of SL2 cells by L.monocytogenes.

(Agaisse et al., 2005)

Origin and Etymology

Discoverer

Etymology

Identification

External Crossreferences and Linkouts ( 56 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

31362

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

Q960X4

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0026080

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG6121

Flygut - An atlas of the Drosophila adult midgut

FBgn0026080

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

31362

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG6121

modMine - A data warehouse for the modENCODE project

FBgn0026080

Linkouts

BioGRID - A database of protein and genetic interactions.

57879

DPiM - Drosophila Protein interaction map

FBgn0026080

DroID - A comprehensive database of gene and protein interactions.

FBgn0026080

DRSC - Results frm RNAi screens

FBgn0026080

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0026080

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG6121-RA

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0026080

FlyMine - An integrated database for Drosophila genomics

FBgn0026080

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

31362

MIST (genetic) - An integrated Molecular Interaction Database

Tip60

MIST (protein-protein) - An integrated Molecular Interaction Database

Tip60

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Synonyms and Secondary IDs (17)

Reported As

Symbol Synonym

6121

(Ravi et al., 2009)

CG6121

(Hosono et al., 2015, Ni et al., 2011.7.19, Keleman et al., 2009.8.5, Perkins et al., 2009, Chen et al., 2008, Zhu et al., 2007, Agaisse et al., 2005, de la Cruz et al., 2005, Kusch et al., 2004, Yang, 2004, Brody et al., 2002)

DmEG0007.7

(Pannuti and Lucchesi, 2000)

Dmel/TIP60

(Toth and Elefant, 2007, Zhu and Elefant, 2007)

Dmel\TIP60

(Zhu et al., 2007)

EG0007.7

(Pilot et al., 2006, Sanjuan and Marin, 2001)

EG:EG0007.7

(Giot et al., 2003)

TIP-60

(Bouazoune and Brehm, 2006)

TIP60

(Scacchetti et al., 2020, Perez-Perri et al., 2016, Puram and Bonni, 2013, Filion et al., 2010)

Tip60

(Beaver et al., 2020, Kennison, 2020.10.22, Mahesh et al., 2020, Zhang et al., 2020, Bailetti et al., 2019, Herrera and Bach, 2019, Shimaji et al., 2019, Shokri et al., 2019, Bischof et al., 2018, McCarthy et al., 2018, Rust et al., 2018, Feng et al., 2017, Merigliano et al., 2017, Transgenic RNAi Project members, 2017-, Börner and Becker, 2016, Hirano et al., 2016, Nakajima et al., 2016, Schwartz et al., 2016, Schertel et al., 2015, Xu and Elefant, 2015, Xu et al., 2014, Yasunaga et al., 2014, Kwon et al., 2013, Neumüller et al., 2013, Smith et al., 2013, Japanese National Institute of Genetics, 2012.5.21, Pirooznia et al., 2012, Lorbeck et al., 2011, Mourikis et al., 2010, Schiemann et al., 2010, Schirling et al., 2010, Clapier and Cairns, 2009, Chen et al., 2008, FlyBase Curators, 2008-, Jin, 2005)

dTIP60

(Pirooznia et al., 2012, Sarthi and Elefant, 2011, Qi et al., 2006)

dTip60

(Giaimo et al., 2018, Xu et al., 2016, Xu et al., 2014, Johnson et al., 2013, Pirooznia et al., 2012, Lorbeck et al., 2011, Hanai et al., 2008, Kaeser and Emerson, 2006, Agaisse et al., 2005, Jin, 2005, Kusch et al., 2004, Kusch et al., 2004)

dmHAG405

(Pandey et al., 2002)

tip60

(Janssens et al., 2017)

Name Synonyms

Tat interactive protein 60kDa

Tat-interactive protein

(Kaeser and Emerson, 2006)

Tat-interactive protein-60 KDa

(Lorbeck et al., 2011)

Tip60

Secondary FlyBase IDs

Datasets (1)

Study focus (1)

Experimental Role

Project

Project Type

Title

transgene_used

FBGSE22069

genome binding

Protein profiling reveals five principal chromatin types in Drosophila cells.

References (102)

The following external sites may use different assemblies or annotations than FlyBase.

Report Sections