FB2026_02 , released June 18, 2026
Gene: Dmel\msps
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General Information
Symbol
Dmel\msps
Species
D. melanogaster
Name
mini spindles
Annotation Symbol
CG5000
Feature Type
protein_coding_gene
FlyBase ID
FBgn0027948
Gene Model Status
Current
Stock Availability
13 publicly available
Gene Summary
Binds to the plus end of microtubules and regulates microtubule dynamics and microtubule organization (Probable) (PubMed:10477755, PubMed:11433295, PubMed:15530399, PubMed:15775959, PubMed:16303556, PubMed:26953351, PubMed:32066907). Function in neurons is essential for adult survival, and is important for climbing behavior and activity (PubMed:37041188). Promotes cytoplasmic microtubule nucleation and elongation (PubMed:16303556, PubMed:17889670). May act as a microtubule antipause factor that rapidly catalyzes the transition from pause to either growth or shrinkage (PubMed:15775959). Involved in mitotic spindle elongation (PubMed:16303556). Involved in the establishment of cell polarity and mitotic spindle orientation in neuroblasts (PubMed:26953351). Required for maintaining the bipolarity of acentrosomal meiotic spindles; the function is dependent on tacc and involves ncd (PubMed:11433295). Involved in oocyte microtubule cytoskeleton organization and bicoid mRNA localization (PubMed:15530399). Seems to be involved in elongation of kinetochore-derived microtubule fibers (PubMed:19836241). In fat body cells, essential component of perinuclear non-centrosomal microtubule-organizing centers (ncMTOCs) which function to accommodate the organization of microtubule (MT) networks to control nuclear positioning and dynein motor-based retrograde endosomal trafficking (PubMed:32066907). Within the ncMTOCs, Msp300 and shot anchors the ncMTOC at the nuclear surface and recruits the MT minus-end regulators Patronin and Nin for assembly, anchoring and/or stabilization of circumferential and radial MTs at the ncMTOCs (PubMed:32066907). Patronin, and perhaps Nin, then recruits msps to the ncMTOC where it is required for the gamma-tubulin-independent elongation and assembly of radial MTs (PubMed:32066907). (UniProt, Q9VEZ3)
Contribute a Gene Snapshot for this gene.
All Summaries
Also Known As

XMAP215, Dis1, TOG

Key Links
Genomic Location
Cytogenetic map
89B1-89B2
Sequence location
Recombination map
3-58
RefSeq locus
NT_033777 REGION:16050801..16060675
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
Gene Ontology (GO) Annotations (27 terms)
Molecular Function (4 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
enables protein binding
inferred from physical interaction with FLYBASE:tacc; FB:FBgn0026620
(Lee et al., 2001)
inferred from physical interaction with UniProtKB:Q9W060
(Lattao et al., 2021)
enables tubulin binding
inferred from direct assay
(Slep and Vale, 2007)
Terms Based on Predictions or Assertions (3 terms)
CV Term
Evidence
References
enables microtubule plus end polymerase activity
inferred from biological aspect of ancestor with PANTHER:PTN000288762
(GO Reference Genome Project, 2011-)
inferred from electronic annotation with InterPro:IPR045110
(InterPro Project Members, 2004-)
enables microtubule plus-end binding
inferred from electronic annotation with InterPro:IPR045110
(InterPro Project Members, 2004-)
enables tubulin binding
inferred from electronic annotation with InterPro:IPR048491
(InterPro Project Members, 2004-)
Biological Process (18 terms)
Terms Based on Experimental Evidence (13 terms)
CV Term
Evidence
References
involved_in axon guidance
inferred from mutant phenotype
(Lowery et al., 2010)
involved_in bicoid mRNA localization
inferred from mutant phenotype
(Moon and Hazelrigg, 2004)
involved_in cytoplasmic microtubule organization
inferred from mutant phenotype
(Pokrywka et al., 2009, Brittle and Ohkura, 2005, Moon and Hazelrigg, 2004)
involved_in endoplasmic reticulum organization
inferred from mutant phenotype
(Pokrywka et al., 2009)
involved_in establishment of mitotic spindle orientation
inferred from mutant phenotype
(Chen et al., 2016)
involved_in establishment or maintenance of neuroblast polarity
inferred from mutant phenotype
(Chen et al., 2016)
involved_in female meiotic nuclear division
inferred from mutant phenotype
(Cullen and Ohkura, 2001)
involved_in mitotic cell cycle
inferred from high throughput mutant phenotype
(Ducat et al., 2008)
involved_in mitotic spindle elongation
inferred from mutant phenotype
(Goshima et al., 2005)
involved_in oocyte microtubule cytoskeleton organization
inferred from mutant phenotype
(Moon and Hazelrigg, 2004)
involved_in oogenesis
inferred from mutant phenotype
(Pokrywka et al., 2009)
involved_in positive regulation of microtubule nucleation
inferred from direct assay
(Slep and Vale, 2007)
involved_in pronuclear fusion
inferred from mutant phenotype
(Lee et al., 2001)
Terms Based on Predictions or Assertions (5 terms)
CV Term
Evidence
References
involved_in centrosome duplication
inferred from biological aspect of ancestor with PANTHER:PTN000288762
(GO Reference Genome Project, 2011-)
involved_in establishment or maintenance of microtubule cytoskeleton polarity
inferred from biological aspect of ancestor with PANTHER:PTN000288762
(GO Reference Genome Project, 2011-)
inferred from electronic annotation with InterPro:IPR045110
(InterPro Project Members, 2004-)
involved_in microtubule polymerization
inferred from electronic annotation with InterPro:IPR045110
(InterPro Project Members, 2004-)
inferred from biological aspect of ancestor with PANTHER:PTN000288762
(GO Reference Genome Project, 2011-)
involved_in mitotic spindle organization
inferred from biological aspect of ancestor with PANTHER:PTN000288762
(GO Reference Genome Project, 2011-)
involved_in spindle organization
inferred from electronic annotation with InterPro:IPR045110
(InterPro Project Members, 2004-)
Cellular Component (5 terms)
Terms Based on Experimental Evidence (3 terms)
CV Term
Evidence
References
located_in centrosome
inferred from direct assay
(Lattao et al., 2021)
located_in microtubule plus-end
inferred from direct assay
(Slep and Vale, 2007)
located_in spindle
inferred from direct assay
(Lattao et al., 2021)
Terms Based on Predictions or Assertions (3 terms)
CV Term
Evidence
References
is_active_in centrosome
inferred from biological aspect of ancestor with PANTHER:PTN000288762
(GO Reference Genome Project, 2011-)
located_in centrosome
traceable author statement
(Lee et al., 2001)
is_active_in kinetochore
inferred from biological aspect of ancestor with PANTHER:PTN000288762
(GO Reference Genome Project, 2011-)
is_active_in spindle pole
inferred from biological aspect of ancestor with PANTHER:PTN000288762
(GO Reference Genome Project, 2011-)
Gene Group (FlyBase)
Protein Family (UniProt)
Belongs to the TOG/XMAP215 family. (Q9VEZ3)
Protein Signatures (InterPro)
Summaries
Gene Group (FlyBase)
MICROTUBULE PLUS END POLYMERASES -
Microtubule plus end polymerases catalyse the transfer of tubulin dimers to the plus end of a microtubule.
Protein Function (UniProtKB)
Binds to the plus end of microtubules and regulates microtubule dynamics and microtubule organization (Probable) (PubMed:10477755, PubMed:11433295, PubMed:15530399, PubMed:15775959, PubMed:16303556, PubMed:26953351, PubMed:32066907). Function in neurons is essential for adult survival, and is important for climbing behavior and activity (PubMed:37041188). Promotes cytoplasmic microtubule nucleation and elongation (PubMed:16303556, PubMed:17889670). May act as a microtubule antipause factor that rapidly catalyzes the transition from pause to either growth or shrinkage (PubMed:15775959). Involved in mitotic spindle elongation (PubMed:16303556). Involved in the establishment of cell polarity and mitotic spindle orientation in neuroblasts (PubMed:26953351). Required for maintaining the bipolarity of acentrosomal meiotic spindles; the function is dependent on tacc and involves ncd (PubMed:11433295). Involved in oocyte microtubule cytoskeleton organization and bicoid mRNA localization (PubMed:15530399). Seems to be involved in elongation of kinetochore-derived microtubule fibers (PubMed:19836241). In fat body cells, essential component of perinuclear non-centrosomal microtubule-organizing centers (ncMTOCs) which function to accommodate the organization of microtubule (MT) networks to control nuclear positioning and dynein motor-based retrograde endosomal trafficking (PubMed:32066907). Within the ncMTOCs, Msp300 and shot anchors the ncMTOC at the nuclear surface and recruits the MT minus-end regulators Patronin and Nin for assembly, anchoring and/or stabilization of circumferential and radial MTs at the ncMTOCs (PubMed:32066907). Patronin, and perhaps Nin, then recruits msps to the ncMTOC where it is required for the gamma-tubulin-independent elongation and assembly of radial MTs (PubMed:32066907).
(UniProt, Q9VEZ3)
Summary (Interactive Fly)

microtubule plus end binding protein - stimulates microtubule growth, microtubule destabilising activity, mitotic spindle

Gene Model and Products
Number of Transcripts
5
Number of Unique Polypeptides
4

Please see the JBrowse view of Dmel\msps for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Structure
Protein 3D structure   (Predicted by AlphaFold)   (AlphaFold entry Q9VEZ3)

If you don't see a structure in the viewer, refresh your browser.
Model Confidence:
  • Very high (pLDDT > 90)
  • Confident (90 > pLDDT > 70)
  • Low (70 > pLDDT > 50)
  • Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures
Crossreferences
PDB - An information portal to biological macromolecular structures
Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Annotated transcripts do not represent all supported alternative splices within 5' UTR.

Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.

Gene model reviewed during 5.47

Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
msps-RB
FBtr0301479
NM_001170156
7455
2050
msps-RC
FBtr0301480
NM_169698
7431
2042
msps-RD
FBtr0304801
NM_001260200
7551
2082
msps-RE
FBtr0304802
NM_001260201
7527
2074
msps-RF
FBtr0304803
NM_001260202
7118
2050
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
UniProt
RefSeq ID
GenBank
msps-PB
FBpp0290694
226.8
2050
8.27
E1JIM4
NP_001163627
ACZ94923
msps-PC
FBpp0290695
226.0
2042
8.21
Q9VEZ3
NP_732105
AAF55269
msps-PD
FBpp0293341
230.4
2082
8.39
A0A0B4K700
NP_001247129
AFH06447
msps-PE
FBpp0293342
229.5
2074
8.33
A0A0B4K664
NP_001247130
AFH06448
msps-PF
FBpp0293343
226.8
2050
8.27
E1JIM4
NP_001247131
AFH06449
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

2050 aa isoforms: msps-PB, msps-PF
Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Subunit Structure (UniProtKB)

Interacts with tacc, dgt6 (PubMed:11433295, PubMed:19836241). Interacts with mv (PubMed:33725482). Interacts with Patronin (PubMed:32066907).

(UniProt, Q9VEZ3)
Domain

The TOG (tumor overexpressed gene) domains are arranged in a N-terminal pentameric array with each domain composed of six (for the most part non-canonical) HEAT repeats forming a oblong paddle-like structure. Intra-HEAT loops are positioned along a face of the TOG domain and bind to a single alpha/beta-tubulin heterodimer. The TOG domains in the array seem to be structurally and functionally polarized. Differential functions may range from microtubule (MT) lattice binding and/or free tubulin heterodimer binding to potentiating stable incorporation of tubulin into the MT lattice. TOG 1-2 show strong and TOG 3-4 weak tubulin binding; TOG 1-5 are required for full ability to promote MT polymerization.

(UniProt, Q9VEZ3)
Crossreferences
InterPro - A database of protein families, domains and functional sites
PDB - An information portal to biological macromolecular structures
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\msps using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.07

Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 1 -- 3
organism

Comment: maternally deposited

(Fisher et al., 2012)
embryonic stage 4 -- 6
central brain anlage in statu nascendi
(Fisher et al., 2012)
antennal anlage in statu nascendi

Comment: reported as procephalic ectoderm anlage in statu nascendi

(Fisher et al., 2012)
dorsal head epidermis anlage in statu nascendi

Comment: reported as procephalic ectoderm anlage in statu nascendi

(Fisher et al., 2012)
visual anlage in statu nascendi

Comment: reported as procephalic ectoderm anlage in statu nascendi

(Fisher et al., 2012)
embryonic stage 7 -- 8
antennal anlage

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)
central brain anlage

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)
dorsal head epidermis anlage

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)
visual anlage

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)
embryonic stage 9 -- 10
antennal primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)
central brain primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)
visual primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)
dorsal head epidermis primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)
lateral head epidermis primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)
ventral head epidermis primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)
ventral nerve cord primordium
(Fisher et al., 2012)
embryonic stage 11 -- 12
central brain primordium
(Fisher et al., 2012)
embryonic central brain glial cell
(Fisher et al., 2012)
embryonic central brain neuron
(Fisher et al., 2012)
embryonic neuroblast of ventral nerve cord primordium
(Fisher et al., 2012)
larval lateral VNC glial cell
(Fisher et al., 2012)
lateral cord neuron
(Fisher et al., 2012)
procephalic neuroblast
(Fisher et al., 2012)
ventral nerve cord primordium
(Fisher et al., 2012)
embryonic stage 13 -- 16
embryonic brain
(Fisher et al., 2012)
larval nervous system
(Fisher et al., 2012)
larval ventral nerve cord
(Fisher et al., 2012)
oogenesis stage S1 -- S9
oocyte
(Moon and Hazelrigg, 2004)
oogenesis stage S8 -- S9
oocyte | anterior
(Moon and Hazelrigg, 2004)
oogenesis stage S10 -- S11
nurse cell
(Moon and Hazelrigg, 2004)
oogenesis stage S12 -- S14
oocyte
(Moon and Hazelrigg, 2004)
Additional Descriptive Data

msps is expressed throughout oogenesis, in both germ cells and follicle cells. In young egg chambers, msps mRNA accumulates in oocytes. At oogenesis stages 8-9, msps mRNA is localized to the anterior cell cortes in oocytes. Expression increases at stage 10, and mRNA is evenly distributed in late-stage (stage 12-14) oocytes.

(Moon and Hazelrigg, 2004)
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
oogenesis stage S1 -- S7
oocyte
(Moon and Hazelrigg, 2004)
oogenesis stage S1 -- S10
nurse cell
(Moon and Hazelrigg, 2004)
oogenesis stage S10
oocyte
(Moon and Hazelrigg, 2004)
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage
adult head
(Aradska et al., 2015)
day 5 of adulthood | male -- day 7 of adulthood | male
spermatozoon
(Wasbrough et al., 2010)
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
located_in centrosome
inferred from direct assay
(Lattao et al., 2021)
located_in microtubule plus-end
inferred from direct assay
(Slep and Vale, 2007)
located_in spindle
inferred from direct assay
(Lattao et al., 2021)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\msps in JBrowse
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 17 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 19 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of msps
Transgenic constructs containing regulatory region of msps
Aberrations (Deficiencies and Duplications) ( 1 )
Inferred from experimentation ( 1 )
Gene disrupted in
Df(3R)Exel7328
(Ryder, 2004.4.26)
Inferred from location ( 5 )
Variants
Variant Molecular Consequences
Alleles Representing Disease-Implicated Variants
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
lethal | recessive
lethal - all die before end of P-stage
lethal - all die before end of pupal stage
lethal - all die during P-stage, with Scer\GAL4Mef2.PR
partially lethal - majority die
viable, with Dcr-2UAS.cDa, Scer\GAL4elav.PLu
viable, with Scer\GAL4pnr-MD237
viable, with Scer\GAL4tin.CΔ4
viable (with mspsMJ208)
viable (with mspsP)
Sterility
Allele
female sterile
female sterile (with mspsMJ208)
female sterile (with mspsP)
Other Phenotypes
Allele
abnormal developmental rate | third instar larval stage
abnormal mitotic cell cycle | third instar larval stage
abnormal mitotic cell cycle | third instar larval stage (with mspsP18)
abnormal mitotic cell cycle | third instar larval stage (with mspsP)
abnormal neuroanatomy, with Scer\GAL4elav.PLu
abnormal neuroanatomy | larval stage, with Scer\GAL4RapGAP1-OK6
abnormal size | heat sensitive, with Scer\GAL4GMR.PF
abnormal size | third instar larval stage
decreased size | embryonic stage
decreased size | larval stage (with msps146)
decreased size | larval stage (with mspsA)
increased cell number | third instar larval stage
increased cell number | third instar larval stage, with Scer\GAL4ase.neuro
increased cell number | third instar larval stage, with Scer\GAL4wor.PA, Scer\GAL80ase.PN
increased cell number | third instar larval stage (with mspsP18)
increased cell number | third instar larval stage (with mspsP)
some die during pharate adult stage
some die during pupal stage
visible
visible, with Scer\GAL4pnr-MD237
wild-type
Phenotype manifest in
Allele
adult cuticle & abdomen
axon | embryonic stage
axon | larval stage (with msps146)
axon | larval stage (with mspsA)
axon microtubule bundle | embryonic stage
axon microtubule bundle | larval stage (with msps146)
axon microtubule bundle | larval stage (with mspsA)
cytoplasmic microtubule & embryonic/larval hemocyte
dendrite | larval stage | conditional, with Scer\GAL4477
eye
follicle cell & microtubule (with mspsMJ208)
follicle cell & microtubule (with mspsP)
imaginal disc | third instar larval stage
larval dorsal multidendritic neuron ddaC | larval stage | conditional, with Scer\GAL4477
larval dorsal multidendritic neuron ddaE | larval stage | conditional, with Scer\GAL4221
larval intersegmental nerve branch ISNb of A1-7, with Scer\GAL4insc-Mz1407
larval MP1 neuron, with Scer\GAL4elav.PLu
larval neuroblast | increased number | third instar larval stage
larval neuroblast | increased number | third instar larval stage, with Scer\GAL4ase.neuro
larval neuroblast | increased number | third instar larval stage, with Scer\GAL4wor.PA, Scer\GAL80ase.PN
larval neuroblast | increased number | third instar larval stage (with mspsP18)
larval neuroblast | increased number | third instar larval stage (with mspsP)
lateral longitudinal fascicle, with Scer\GAL4elav.PLu
macrochaeta
mesonotum, with Scer\GAL4pnr-MD237
microtubule & nurse cell | oogenesis stage S8 (with mspsMJ208)
microtubule & nurse cell | oogenesis stage S8 (with mspsP)
microtubule & nurse cell | oogenesis stage S9 (with mspsMJ208)
microtubule & nurse cell | oogenesis stage S9 (with mspsP)
microtubule & nurse cell | oogenesis stage S10B (with mspsMJ208)
microtubule & nurse cell | oogenesis stage S10B (with mspsP)
microtubule & oocyte | oogenesis stage S10B (with mspsMJ208)
microtubule & oocyte | oogenesis stage S10B (with mspsP)
microtubule plus-end | embryonic stage
mitotic anaphase
mitotic anaphase & nuclear chromosome
mitotic cell cycle
mitotic cell cycle & spindle
mitotic spindle | third instar larval stage
NMJ bouton | larval stage, with Scer\GAL4RapGAP1-OK6
nurse cell & microtubule (with mspsMJ208)
nurse cell & microtubule (with mspsP)
oocyte & microtubule (with mspsMJ208)
oocyte & microtubule (with mspsP)
oocyte & spindle
oocyte | oogenesis stage S7 (with mspsMJ208)
oocyte | oogenesis stage S7 (with mspsP)
oocyte nucleus | oogenesis stage S10 (with mspsMJ208)
oocyte nucleus | oogenesis stage S10 (with mspsP)
oocyte nucleus | oogenesis stage S11 (with mspsMJ208)
oocyte nucleus | oogenesis stage S11 (with mspsP)
oocyte nucleus | oogenesis stage S12 (with mspsMJ208)
oocyte nucleus | oogenesis stage S12 (with mspsP)
pronucleus
retina | heat sensitive, with Scer\GAL4GMR.PF
spindle
spindle | third instar larval stage
spindle | third instar larval stage (with mspsP18)
spindle | third instar larval stage (with mspsP)
type II neuroblast | somatic clone | third instar larval stage
type II neuroblast | third instar larval stage, with Scer\GAL4wor.PA, Scer\GAL80ase.PN
type I neuroblast | somatic clone | third instar larval stage
type I neuroblast | third instar larval stage, with Scer\GAL4ase.neuro
Orthologs
Downloads
Download All DIOPT Orthologs
Human Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Homo sapiens (Human) (1)
Hsap\CKAP5
14 of 14
Yes
Yes
Model Organism Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Rattus norvegicus (Norway rat) (1)
Rnor\Ckap5
13 of 14
Yes
Yes
Mus musculus (laboratory mouse) (1)
Mmus\Ckap5
13 of 14
Yes
Yes
Xenopus tropicalis (Western clawed frog) (3)
Xtro\ckap5
8 of 13
Yes
Yes
Xtro\nrros
1 of 13
No
No
Xtro\sgta
1 of 13
No
No
Danio rerio (Zebrafish) (1)
Drer\ckap5
13 of 14
Yes
Yes
Caenorhabditis elegans (Nematode, roundworm) (3)
Cele\zyg-9
9 of 14
Yes
Yes
Cele\F54A3.2
7 of 14
No
Yes
Cele\T08D2.8
3 of 14
No
Yes
Anopheles gambiae (African malaria mosquito) (1)
Agam\AgaP_AGAP011162
12 of 12
Yes
Yes
Arabidopsis thaliana (thale-cress) (2)
Atha\MOR1
13 of 13
Yes
Yes
Atha\AT3G28155
2 of 13
No
Yes
Saccharomyces cerevisiae (Brewer's yeast) (1)
Scer\STU2
6 of 13
Yes
Yes
Schizosaccharomyces pombe (Fission yeast) (2)
Spom\alp14
7 of 12
Yes
Yes
Spom\dis1
5 of 12
No
Yes
Escherichia coli (enterobacterium) (0)
Other Organism Orthologs (via OrthoDB)
Data provided directly from OrthoDB:msps. Refer to their site for version information.
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v9.1)
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 0 )
    Human Ortholog
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Interaction
    References
    Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    CKAP5; cytoskeleton associated protein 5
    14 of 14
    611142
        Functional Complementation Data
        Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
        Interactions
        Summary of Physical Interactions
        Interaction Browsers
        View in FlyBase Interactions Browser   View in MIST tool (external link)
        Please see the Physical Interaction reports below for full details
        protein-protein
        Physical Interaction
        Assay
        References
        msps - Arf1
        proximity ligation assay, fluorescence microscopy, bimolecular fluorescence complementation
        (Gujar et al., 2023)
        msps - CG10164
        experimental knowledge based
        (Guruharsha et al., 2011)
        msps - CycB
        anti bait coimmunoprecipitation, western blot, pull down
        (Basto et al., 2007)
        msps - Hsp83
        anti bait coimmunoprecipitation, western blot
        (Basto et al., 2007)
        msps - Patronin
        anti tag coimmunoprecipitation, western blot
        (Zheng et al., 2020)
        msps - chb
        anti bait coimmunoprecipitation, western blot, anti tag coimmunoprecipitation
        (Trogden and Rogers, 2015)
        msps - dgt6
        anti bait coimmunoprecipitation, western blot
        (Bucciarelli et al., 2009)
        msps - mael
        anti bait coimmunoprecipitation, peptide massfingerprinting, western blot
        (Sato et al., 2011)
        msps - mars
        anti bait coimmunoprecipitation, western blot
        (Tan et al., 2008)
        msps - mv
        anti tag coimmunoprecipitation, western blot, Identification by mass spectrometry
        (Lattao et al., 2021)
        msps - ssp2
        anti tag coimmunoprecipitation, anti tag western blot
        (Li et al., 2012)
        msps - tacc
        anti bait coimmunoprecipitation, western blot, anti tag coimmunoprecipitation, anti tag western blot, pull down, Identification by mass spectrometry, coimmunoprecipitation, experimental knowledge based
        (Tang et al., 2020, Guruharsha et al., 2011, Barros et al., 2005, Lee et al., 2001)
        Summary of Genetic Interactions
        Interaction Browsers
        View in FlyBase Interactions Browser   View in MIST tool (external link)
        Please look at the allele data for full details of the genetic interactions
        Starting gene(s)
        Interaction type
        Interacting gene(s)
        Reference
        msps
        suppressible
        Abl
        (Lowery et al., 2010)
        msps
        suppressible
        ball
        (Cullen et al., 2005)
        Eb1|tau|msps
        suppressible
        shot
        (Hahn et al., 2021)
        Starting gene(s)
        Interaction type
        Interacting gene(s)
        Reference
        Abl
        suppressible
        msps
        (Lowery et al., 2010)
        Arl2
        suppressible
        msps
        (Chen et al., 2016)
        chb
        suppressible
        msps
        (Lowery et al., 2010)
        chb
        enhanceable
        msps
        (Lowery et al., 2010)
        mv
        enhanceable
        msps
        (Lattao et al., 2021)
        External Data
        Subunit Structure (UniProtKB)
        Interacts with tacc, dgt6 (PubMed:11433295, PubMed:19836241). Interacts with mv (PubMed:33725482). Interacts with Patronin (PubMed:32066907).
        (UniProt, Q9VEZ3 )
        Linkouts
        BioGRID - A database of protein and genetic interactions.
        DroID - A comprehensive database of gene and protein interactions.
        MIST (genetic) - An integrated Molecular Interaction Database
        MIST (protein-protein) - An integrated Molecular Interaction Database
        Pathways
        Signaling Pathways (FlyBase)
        Metabolic Pathways
        FlyBase
        External Links
        External Data
        Linkouts
        Class of Gene
        Genomic Location and Detailed Mapping Data
        Chromosome (arm)
        3R
        Recombination map
        3-58
        Cytogenetic map
        89B1-89B2
        Sequence location
        FlyBase Computed Cytological Location
        Cytogenetic map
        Evidence for location
        89B1-89B2
        Limits computationally determined from genome sequence between P{PZ}blp01618 and P{PZ}gish04895&P{EP}EP3171
        Experimentally Determined Cytological Location
        Cytogenetic map
        Notes
        References
        89B-89B
        (Ohkura, 1999.7.30)
        89B-89B
        (determined by in situ hybridisation)
        (Cullen et al., 1999)
        Experimentally Determined Recombination Data
        Location

        3-58

        (FlyBase, 2016)

        3-57.9

        (Comeron et al., 2012)
        Left of (cM)
        Right of (cM)
        Notes
        Stocks and Reagents
        Stocks (13)
        Genomic Clones (25)
        cDNA Clones (68)
         

        Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

        cDNA clones, fully sequenced
        BDGP DGC clones
        Other clones
          Drosophila Genomics Resource Center cDNA clones

          For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

          cDNA Clones, End Sequenced (ESTs)
          BDGP DGC clones
          Other clones
          RNAi and Array Information
          Linkouts
          DRSC - Results frm RNAi screens
          Antibody Information
          Laboratory Generated Antibodies
           

          polyclonal

          (Lattao et al., 2021, Currie et al., 2011, Kao and Megraw, 2009)
          Commercially Available Antibodies
           
          Cell Line Information
          Publicly Available Cell Lines
           
          Other Stable Cell Lines
           
            Other Comments

            S2 cells treated with RNAi against msps show reduced poleward flux rates of tubulin subunits in mitotic spindles and sharply decreased α-tubulin turnover at the microtubule plus and minus ends of the spindles. There is a significantly increased scatter of chromosomes throughout the spindle in the RNAi treated cells compared to controls. Pole-to-pole spindle lengths are significantly reduced in the RNAi treated cells compared to controls.

            (Buster et al., 2007)

            RNAi screen using dsRNA made from templates generated with primers directed against this gene results in aberrantly short spindles when assayed in S2 cells. This phenotype can be observed when the screen is performed with or without Cdc27 dsRNA.

            (Goshima et al., 2007)

            S2 cells transfected with dsRNA made from templates generated with primers directed against this gene show shortening of the metaphase spindle.

            (Goshima et al., 2005)

            msps is required during oogenesis for the normal structure and function of cytoplasmic microtubules.

            (Moon and Hazelrigg, 2004)

            dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.

            (Kiger et al., 2003)

            sub mutants do not interact with alleles of γTub37C, αTub67C, msps, tacc or wisp.

            (Giunta et al., 2002)

            msps is required for the integrity of the mitotic spindle.

            (Cullen et al., 1999)

            Mutation in msps disrupts the integrity of the mitotic spindle, resulting in the formation of one or more small additional spindles in diploid cells.

            (Cullen et al., 1999)
            Relationship to Other Genes
            Source for database merge of

            Source for merge of: msps anon-WO0140519.136

            (FlyBase, 1996-)
            Additional comments

            Source for merge of msps anon-WO0140519.136 was sequence comparison ( date:051113 ).

            (FlyBase, 1996-)
            Nomenclature History
            Source for database identify of
            Nomenclature comments
            Etymology
            Synonyms and Secondary IDs (16)
            Reported As
            Symbol Synonym
            CG5000
            (Meadows, 2017.1.11, Çiçek et al., 2016, Hosono et al., 2015, Lowery et al., 2010, Müller et al., 2010, Ni et al., 2010.12.1, Ducat et al., 2008, Hughes et al., 2008, Ni et al., 2008, Goshima et al., 2007, Goldstein and Gunawardena, 2000)
            Dis1
            (Currie et al., 2011, Goshima et al., 2005, Odde, 2005)
            MSPS
            (Burgess et al., 2015, Moshkin et al., 2009)
            Msps
            (Gujar and Wang, 2025, Wong et al., 2025, Kloc, 2024, Rui, 2024, Chippalkatti et al., 2020, Pirani et al., 2020, Rui et al., 2020, Zheng et al., 2020, Costa and Ohkura, 2019, Lattao et al., 2017, Chen et al., 2016, Kupriyanova et al., 2016, Ogneva et al., 2016, Howard et al., 2015, Richens et al., 2015, Fahmy et al., 2014, Li et al., 2011, Sato et al., 2011, Wang et al., 2011, Conduit et al., 2010, Kallappagoudar et al., 2010, Lowery et al., 2010, Fernandez et al., 2009, Kao and Megraw, 2009, Moutinho-Pereira et al., 2009, Megraw et al., 2008, Rogers et al., 2008, Buster et al., 2007, Goshima et al., 2007, Slep and Vale, 2007, Tan et al., 2007, Zhang and Megraw, 2007, Basto et al., 2006, Barros et al., 2005, Pearson et al., 2005, Blagden and Glover, 2003, Raff, 2002)
            TOG
            (Goshima et al., 2005, Odde, 2005)
            XMAP215
            (Currie et al., 2011, Bucciarelli et al., 2009, Goshima et al., 2005, Odde, 2005)
            anon-WO0140519.136
            msps
            (Kittock et al., 2026, Lu et al., 2023, Zhu et al., 2023, Beaver et al., 2022, Bu et al., 2022, Cassella and Ephrussi, 2022, Vazquez-Pianzola and Beuchle, 2022.11.20, Deng et al., 2021, Lattao et al., 2021, Vicars et al., 2021, Chou et al., 2020, Karageorgiou et al., 2020, Tang et al., 2020, Blake-Hedges and Megraw, 2019, Hughes et al., 2018, Clandinin and Owens, 2016-, Lee et al., 2016, Dent et al., 2015, Ashwal-Fluss et al., 2014, Perkins and Tanentzapf, 2014, Pokrywka et al., 2014, Kwon et al., 2013, Moshkin et al., 2013, Japanese National Institute of Genetics, 2012.5.21, Currie et al., 2011, Friedman et al., 2011, Stone et al., 2010, Wasbrough et al., 2010, Bucciarelli et al., 2009, Pokrywka et al., 2009, Pokrywka et al., 2008, Somma et al., 2008, Bakal et al., 2007, Basto et al., 2007, Cullen et al., 2005, De Lucia et al., 2005, Still et al., 2004)
            Name Synonyms
            MINISPINDLES
            (Kramer and Hawley, 2003)
            Mini Spindles
            (Slep and Vale, 2007)
            Mini spindles
            (Howard et al., 2015, Currie et al., 2011, Slep and Vale, 2007, Pokrywka and Hazelrigg, 2006)
            Mini-spindles
            (Rogers et al., 2008)
            Minispindles
            (Ducat et al., 2008, Somma et al., 2008, Tan et al., 2007, Barros et al., 2005, Blagden and Glover, 2003)
            mini spindles
            (Applewhite et al., 2013, Li et al., 2011, Pokrywka et al., 2009, Basto et al., 2007, Cullen et al., 2005, Cullen et al., 1999)
            mini-spindles
            (Rogers et al., 2008)
            minispindles
            (Lowery et al., 2010, Fernandez et al., 2009, Tan et al., 2008, Tadros et al., 2007)
            Secondary FlyBase IDs
            • FBgn0044745
            Datasets (0)
            Study focus (0)
            Experimental Role
            Project
            Project Type
            Title
            Study result (0)
            Result
            Result Type
            Title
            External Crossreferences and Linkouts ( 68 )
            Sequence Crossreferences
            NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
            GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
            GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
            RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
            UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.
            UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
            UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
            Other crossreferences
            AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.
            BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
            DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
            EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
            FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
            FlyMine - An integrated database for Drosophila genomics
            InterPro - A database of protein families, domains and functional sites
            KEGG Genes - Molecular building blocks of life in the genomic space.
            MARRVEL_MODEL - MARRVEL (model organism gene)
            PDB - An information portal to biological macromolecular structures
            Linkouts
            BioGRID - A database of protein and genetic interactions.
            Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
            DroID - A comprehensive database of gene and protein interactions.
            DRSC - Results frm RNAi screens
            Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
            FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
            FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
            Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
            Flygut - An atlas of the Drosophila adult midgut
            iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
            Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
            MIST (genetic) - An integrated Molecular Interaction Database
            MIST (protein-protein) - An integrated Molecular Interaction Database
            References (179)