dMBD2/3, MBD2/3, MBD3, MBD2
Gene model reviewed during 5.47
1.2, 1 (northern blot)
None of the polypeptides share 100% sequence identity.
30, 25 (kD)
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\MBD-like using the Feature Mapper tool.
Developmental western analysis shows that the long MBD-like isoform is present in embryonic extracts from 0-12 hours of development.
Developmental western analysis shows that the short MBD-like isoform is present in embryonic extracts from 6-20 hours of development.
Homogenous expression of MBD-like protein is observed in cleavage embryos. The staining intensity increases and staining is cytoplasmic. At cellular blastoderm stage there is a transient colocalization of the protein and DNA indicating a nuclear localization of MBD-like protein at this stage. However in gastrula and later embryonic development protein staining is detected primarily in the cytoplasm and nuclear periphery. Nuclear accumulation of MBD-like protein coincides with zygotic genome activation. Nuclear MBD-like protein was also observed in primary spermatocytes at the time of Y-chromosome activation. The protein was predominately cytoplasmic at earlier stages of spermatogenesis.
GBrowse - Visual display of RNA-Seq signalsView Dmel\MBD-like in GBrowse 2
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Source for identity of: methyl-CpG-binding-domain-like-protein MBD2-3
Shows particularly robust cycling of transcription in adult heads, as assessed by expression analysis using high density oligonucleotide arrays with probe generated during three 12-point time course experiments over the course of 6 days.