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General Information
Symbol
Dmel\Atg5
Species
D. melanogaster
Name
Autophagy-related 5
Annotation Symbol
CG1643
Feature Type
FlyBase ID
FBgn0029943
Gene Model Status
Stock Availability
Gene Summary
Involved in autophagic vesicle formation. Conjugation with Atg12, through a ubiquitin-like conjugating system involving Atg7 as an E1-like activating enzyme and Atg10 as an E2-like conjugating enzyme, is essential for its function. The Atg12-Atg5 conjugate acts as an E3-like enzyme which is required for lipidation of Atg8 and its association to the vesicle membranes (By similarity). (UniProt, Q9W3R7)
Contribute a Gene Snapshot for this gene.
Also Known As

Autophagy-specific gene 5

Key Links
Genomic Location
Cytogenetic map
Sequence location
X:7,314,622..7,323,770 [-]
Recombination map
1-20
RefSeq locus
NC_004354 REGION:7314622..7323770
Sequence
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
Function
GO Summary Ribbons
Gene Ontology (GO) Annotations (15 terms)
Molecular Function (1 term)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
contributes_to Atg8 ligase activity
inferred from sequence or structural similarity with SGD:S000006070
Biological Process (10 terms)
Terms Based on Experimental Evidence (5 terms)
CV Term
Evidence
References
involved_in autophagy
inferred from mutant phenotype
inferred from mutant phenotype
involved_in glycophagy
inferred from mutant phenotype
involved_in macroautophagy
inferred from mutant phenotype
Terms Based on Predictions or Assertions (7 terms)
CV Term
Evidence
References
inferred from biological aspect of ancestor with PANTHER:PTN000315667
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN000315667
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN000315667
(assigned by GO_Central )
involved_in autophagy
inferred from sequence or structural similarity with UniProtKB:Q99J83
(assigned by UniProt )
inferred from biological aspect of ancestor with PANTHER:PTN000315667
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN000315667
(assigned by GO_Central )
involved_in macroautophagy
inferred from sequence or structural similarity with SGD:S000006070
Cellular Component (4 terms)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
located_in autophagosome
inferred from direct assay
Terms Based on Predictions or Assertions (3 terms)
CV Term
Evidence
References
inferred from sequence or structural similarity with SGD:S000006070
inferred from biological aspect of ancestor with PANTHER:PTN000315667
(assigned by GO_Central )
located_in cytoplasm
inferred from sequence or structural similarity with UniProtKB:Q99J83
(assigned by UniProt )
inferred from biological aspect of ancestor with PANTHER:PTN000315667
(assigned by GO_Central )
Protein Family (UniProt)
Belongs to the ATG5 family. (Q9W3R7)
Summaries
Gene Group (FlyBase)
AUTOPHAGY-RELATED GENES -
The Autophagy-related genes (Atg), characterized extensively in yeast, are a set of approximately twenty evolutionarily conserved genes required for autophagy. Autophagy (specifically, macroautophagy) is the process of bulk degradation of cytoplasmic material. It takes place by the assembly of a double membrane vesicle, the autophagosome, which delivers cargo to the lysosome for recycling. (Adapted from FBrf0219844 and FBrf020902).
ATG12-ATG5-ATG16 COMPLEX -
The Atg12-Atg5-Atg16 complex is needed for autophagosome formation. It is composed of Atg16 interacting with covalently linked Atg12-Atg5. (Adapted from PMID:23321721 and PMID:23999079).
ATG8 TRANSFERASES -
Atg8 transferases catalyse the transfer of the ubiquitin-like protein Atg8 from one substrate to another. Analogous to ubiquitination, Atg8 is first activated by the E1-like enzyme Atg7, and is then transferred to the E2-like enzyme Atg3. In the final step, the E3-like Atg5-Atg12 heterodimer acts to conjugate Atg8 to phosphatidylethanolamine in a membrane-associated form. Atg8 transferase activity is required for autophagosome biogenesis. (Adapted from PMID:18704115.)
Protein Function (UniProtKB)
Involved in autophagic vesicle formation. Conjugation with Atg12, through a ubiquitin-like conjugating system involving Atg7 as an E1-like activating enzyme and Atg10 as an E2-like conjugating enzyme, is essential for its function. The Atg12-Atg5 conjugate acts as an E3-like enzyme which is required for lipidation of Atg8 and its association to the vesicle membranes (By similarity).
(UniProt, Q9W3R7)
Gene Model and Products
Number of Transcripts
1
Number of Unique Polypeptides
1

Please see the JBrowse view of Dmel\Atg5 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model

Gene model reviewed during 5.50

Sequence Ontology: Class of Gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
FBtr0071065
1418
269
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
FBpp0071023
31.5
269
6.08
Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Post Translational Modification

Conjugated to Atg12, which is essential for autophagy.

(UniProt, Q9W3R7)
Crossreferences
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Atg5 using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
organism

Comment: maternally deposited

northern blot
Stage
Tissue/Position (including subcellular localization)
Reference
RT-PCR
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data

qRT-PCR of adult female heads assayed at weekly intervals shows that transcript levels decline significantly by 3 weeks and remain supressed.

The expression of this gene along with several others is induced in salivary glands in pupae at the time of head eversion. This stage is characterized by an increase in the ecdysone titer as well as large amounts of cell death in this tissue.

Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
located_in autophagosome
inferred from direct assay
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Atg5 in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
EMBL-EBI Single Cell Expression Atlas
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 4 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 16 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of Atg5
Transgenic constructs containing regulatory region of Atg5
Aberrations (Deficiencies and Duplications) ( 0 )
Inferred from experimentation ( 0 )
Inferred from location ( 0 )
Alleles Representing Disease-Implicated Variants
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
Sterility
Allele
Other Phenotypes
Allele
Phenotype manifest in
Allele
Orthologs
Human Orthologs (via DIOPT v8.0)
Homo sapiens (Human) (1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
14 of 15
Yes
Yes
 
2  
Model Organism Orthologs (via DIOPT v8.0)
Mus musculus (laboratory mouse) (1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
13 of 15
Yes
Yes
Rattus norvegicus (Norway rat) (1)
7 of 13
Yes
Yes
Xenopus tropicalis (Western clawed frog) (1)
13 of 12
Yes
Yes
Danio rerio (Zebrafish) (1)
14 of 15
Yes
Yes
Caenorhabditis elegans (Nematode, roundworm) (1)
12 of 15
Yes
Yes
Arabidopsis thaliana (thale-cress) (2)
6 of 9
Yes
Yes
1 of 9
No
Yes
Saccharomyces cerevisiae (Brewer's yeast) (1)
10 of 15
Yes
Yes
Schizosaccharomyces pombe (Fission yeast) (1)
10 of 12
Yes
Yes
Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG09190D1E )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila suzukii
Spotted wing Drosophila
Drosophila simulans
Drosophila sechellia
Drosophila erecta
Drosophila yakuba
Drosophila ananassae
Drosophila pseudoobscura pseudoobscura
Drosophila persimilis
Drosophila willistoni
Drosophila virilis
Drosophila mojavensis
Drosophila grimshawi
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091509HL )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Glossina morsitans
Tsetse fly
Lucilia cuprina
Australian sheep blowfly
Mayetiola destructor
Hessian fly
Aedes aegypti
Yellow fever mosquito
Anopheles darlingi
American malaria mosquito
Culex quinquefasciatus
Southern house mosquito
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W0BDF )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Danaus plexippus
Monarch butterfly
Heliconius melpomene
Postman butterfly
Apis florea
Little honeybee
Apis mellifera
Western honey bee
Bombus impatiens
Common eastern bumble bee
Bombus terrestris
Buff-tailed bumblebee
Linepithema humile
Argentine ant
Megachile rotundata
Alfalfa leafcutting bee
Nasonia vitripennis
Parasitic wasp
Dendroctonus ponderosae
Mountain pine beetle
Tribolium castaneum
Red flour beetle
Pediculus humanus
Human body louse
Rhodnius prolixus
Kissing bug
Cimex lectularius
Bed bug
Cimex lectularius
Bed bug
Acyrthosiphon pisum
Pea aphid
Zootermopsis nevadensis
Nevada dampwood termite
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X0BB3 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strigamia maritima
European centipede
Ixodes scapularis
Black-legged tick
Stegodyphus mimosarum
African social velvet spider
Tetranychus urticae
Two-spotted spider mite
Daphnia pulex
Water flea
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G0JU7 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Ciona intestinalis
Vase tunicate
Gallus gallus
Domestic chicken
Paralogs
Paralogs (via DIOPT v8.0)
Drosophila melanogaster (Fruit fly) (0)
No records found.
Human Disease Associations
FlyBase Human Disease Model Reports
Disease Model Summary Ribbon
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 1 )
Allele
Disease
Evidence
References
Potential Models Based on Orthology ( 1 )
Human Ortholog
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 2 )
Allele
Disease
Interaction
References
Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)
Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
Homo sapiens (Human)
Gene name
Score
OMIM
OMIM Phenotype
DO term
Complementation?
Transgene?
Functional Complementation Data
Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
Dmel gene
Ortholog showing functional complementation
Supporting References
Interactions
Summary of Physical Interactions
Summary of Genetic Interactions
esyN Network Diagram
esyN Network Key:
Suppression
Enhancement

Please look at the allele data for full details of the genetic interactions
Starting gene(s)
Interaction type
Interacting gene(s)
Reference
Starting gene(s)
Interaction type
Interacting gene(s)
Reference
External Data
Linkouts
BioGRID - A database of protein and genetic interactions.
DroID - A comprehensive database of gene and protein interactions.
InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
MIST (protein-protein) - An integrated Molecular Interaction Database
Pathways
Signaling Pathways (FlyBase)
Metabolic Pathways
External Data
Linkouts
Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
Genomic Location and Detailed Mapping Data
Chromosome (arm)
X
Recombination map
1-20
Cytogenetic map
Sequence location
X:7,314,622..7,323,770 [-]
FlyBase Computed Cytological Location
Cytogenetic map
Evidence for location
7B1-7B1
Limits computationally determined from genome sequence between P{EP}CG9650EP1340&P{EP}CG9650EP1617 and P{EP}CBPEP1643&P{EP}EP1523EP1523
Experimentally Determined Cytological Location
Cytogenetic map
Notes
References
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Stocks and Reagents
Stocks (12)
Genomic Clones (9)
 

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (26)
 

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced
BDGP DGC clones
Other clones
Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

cDNA Clones, End Sequenced (ESTs)
RNAi and Array Information
Linkouts
DRSC - Results frm RNAi screens
GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
Antibody Information
Laboratory Generated Antibodies
 
Commercially Available Antibodies
 
Other Information
Relationship to Other Genes
Source for database identify of

Source for identity of: Atg5 CG1643

Source for identity of: atg5 CG1643

Source for database merge of
Additional comments
Other Comments

Disruption of Atg1 or Atg5 prevents induction of autophagy in response to starvation.

Origin and Etymology
Discoverer
Etymology
Identification
External Crossreferences and Linkouts ( 38 )
Sequence Crossreferences
NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
Other crossreferences
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
EMBL-EBI Single Cell Expression Atlas
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
KEGG Genes - Molecular building blocks of life in the genomic space.
MARRVEL_MODEL
modMine - A data warehouse for the modENCODE project
Linkouts
BioGRID - A database of protein and genetic interactions.
DroID - A comprehensive database of gene and protein interactions.
DRSC - Results frm RNAi screens
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
FlyMine - An integrated database for Drosophila genomics
InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
MIST (protein-protein) - An integrated Molecular Interaction Database
Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
Synonyms and Secondary IDs (10)
Reported As
Symbol Synonym
Atg5
(Han et al., 2021, Ma et al., 2021, Barthez et al., 2020, Bjedov and Rallis, 2020, Bjedov et al., 2020, Cunningham et al., 2020, Du et al., 2020, Jacomin et al., 2020, Khan et al., 2020, Kiral et al., 2020, Kiss et al., 2020, Lin et al., 2020, Nagai et al., 2020, Nakajima et al., 2020, Sênos Demarco et al., 2020, Vernizzi et al., 2020, Xu et al., 2020, Aparicio et al., 2019, Bhattacharjee et al., 2019, Bourouis et al., 2019, Bouska et al., 2019, Doktór et al., 2019, Jiang et al., 2019, Khezri and Rusten, 2019, Meltzer et al., 2019, Nilangekar et al., 2019, Sênos Demarco et al., 2019, Sim et al., 2019, Su et al., 2019, Tettamanti and Casartelli, 2019, Yan et al., 2019, Zhang et al., 2019, Billes et al., 2018, Gene Disruption Project members, 2018-, Issa et al., 2018, Kuo et al., 2018, Lee et al., 2018, Levis, 2018.8.30, Liu et al., 2018, Nakamura and Yoshimori, 2018, O'Keefe and Denton, 2018, Swevers et al., 2018, Bali and Shravage, 2017, Galluzzi et al., 2017, Hu et al., 2017.6.13, Kim et al., 2017, Kuleesha et al., 2017, Lei et al., 2017, Lo Piccolo et al., 2017, Manent et al., 2017, Nagy et al., 2017, Paiardi et al., 2017, Péan et al., 2017, Transgenic RNAi Project members, 2017-, Tusco et al., 2017, Yan et al., 2017, Kim et al., 2016, Kuleesha et al., 2016, Liu et al., 2016, Nagy et al., 2016, Sarov et al., 2016, Babcock et al., 2015, Denton et al., 2015, Fan et al., 2015, Kim et al., 2015, Kuhn et al., 2015, Park et al., 2015, Rojas-Ríos et al., 2015, Xu et al., 2015, Yang et al., 2015, Zhang and Baehrecke, 2015, Zirin et al., 2015, Ambrosio et al., 2014, Banreti et al., 2014, Ling et al., 2014, Moy et al., 2014, Mulakkal et al., 2014, Zacharogianni et al., 2014, Bai et al., 2013, Chang et al., 2013, Denton et al., 2013, Levis et al., 2013-, Lin et al., 2013, Moy and Cherry, 2013, Nowak et al., 2013, Shin et al., 2013, Takáts et al., 2013, Wen et al., 2013, Zhang et al., 2013, Zirin et al., 2013, Eleftherianos and Castillo, 2012, Érdi et al., 2012, Garcia et al., 2012, Igboin et al., 2012, Immonen and Ritchie, 2012, Japanese National Institute of Genetics, 2012.5.21, Nakamoto et al., 2012, Taillebourg et al., 2012, Wong et al., 2012, Barth et al., 2011, Lee et al., 2011, Milton et al., 2011, Qin et al., 2011, Yano and Kurata, 2011, Bjedov et al., 2010, Chang and Neufeld, 2010, Chi et al., 2010, Demontis and Perrimon, 2010, Filimonenko et al., 2010, Haberman et al., 2010, Kim et al., 2010, Nezis et al., 2010, Nisoli et al., 2010, Zirin and Perrimon, 2010, Ziviani et al., 2010, Denton et al., 2009, Köhler et al., 2009, Ling et al., 2009, Ren et al., 2009, Shelly et al., 2009, Tain et al., 2009, Chen et al., 2008, Hong et al., 2008, Hou et al., 2008, Liu and Lehmann, 2008, Simonsen et al., 2008, Yano et al., 2008, Bilen and Bonini, 2007, Engström et al., 2007, Quinones-Coello, 2007, Scott et al., 2007, Zeitouni et al., 2007)
Name Synonyms
Autophagy-related 5
autophagy-specific gene 5
Secondary FlyBase IDs
    Datasets (0)
    Study focus (0)
    Experimental Role
    Project
    Project Type
    Title
    References (225)