DmKlp10A, kinesin-13
mitotic kinesin required to depolymerize microtubules at their pole-associated minus ends thereby moving chromatids by means of poleward flux - a stem cell centrosome-enriched kinesin that balances asymmetries in Drosophila male germline stem cell division
Please see the JBrowse view of Dmel\Klp10A for information on other features
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Gene model reviewed during 5.55
Tissue-specific extension of 3' UTRs observed during later stages (FBrf0218523, FBrf0219848); all variants may not be annotated
Gene model reviewed during 5.46
Low-frequency RNA-Seq exon junction(s) not annotated.
Annotated transcripts do not represent all supported alternative splices within 5' UTR.
Gene model reviewed during 5.50
Gene model reviewed during 5.56
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Klp10A using the Feature Mapper tool.
GBrowse - Visual display of RNA-Seq signals
View Dmel\Klp10A in GBrowse 21-33
1-33
1-34.8
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Source for identity of: Klp10A CG1453
Source for merge of: Klp10A anon-WO03040301.142
Source for merge of Klp10A anon-WO03040301.142 was sequence comparison ( date:051113 ).
S2 cells treated with RNAi against Klp10A show reduced poleward flux rates of tubulin subunits in mitotic spindles and suppressed α-tubulin turnover at the microtubule minus ends of the spindles. The chromatid-to-pole velocity during anaphase is decreased in these cells. Pole-to-pole spindle lengths are significantly increased in the RNAi treated cells compared to controls.
RNAi screen using dsRNA made from templates generated with primers directed against this gene results in aberrantly long spindles when assayed in S2 cells. This phenotype can be observed when the screen is performed with or without Cdc27 dsRNA.
S2 cells transfected with dsRNA made from templates generated with primers directed against this gene show an expansion of the metaphase spindle.
Klp10A is required to depolymerise microtubules at their pole associated minus ends, thereby moving chromatids by means of poleward flux.
The introduction of Klp10A inhibitors into embryos rapidly alters the organisation of mitotic spindle microtubules. 30% form monopolar spindles, which assemble when centrosomes collapse back together after nuclear envelope breakdown. About 61% display abnormally long bipolar spindles. These bipolar spindles elongate continuously during prometaphase through anaphase and attain metaphase lengths approximately twice that of controls. The movement and segregation of chromosomes is also perturbed.
dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.
