FlyBase Gene Report: Dmel\Atg9

General Information

Symbol

Dmel\Atg9

Species

D. melanogaster

Name

Autophagy-related 9

Annotation Symbol

CG3615

Feature Type

protein_coding_gene

FlyBase ID

FBgn0034110

Gene Model Status

Stock Availability

5 publicly available

Gene Summary

Autophagy-related 9 (Atg9) encodes an integral membrane protein acting as a membrane carrier for forming autophagosomes. It regulates TOR and JNK signaling pathways via autophagy. [Date last reviewed: 2019-07-11] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
Interactive Fly

Key Links

Genomic Location

Cytogenetic map

Sequence location

2R:16,294,400..16,297,232 [+]

Recombination map

2-79

RefSeq locus

NT_033778 REGION:16294400..16297232

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse GBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

Gene Ontology (GO) Annotations (18 terms)

Molecular Function (0 terms)

There is no information supporting a Molecular Function term for this gene. (Based on a review of available data on: 2012-05-10 )

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (0 terms)

Biological Process (13 terms)

Terms Based on Experimental Evidence (9 terms)

CV Term

Evidence

References

involved_in autophagy

inferred from mutant phenotype

(Wen et al., 2017, Xu et al., 2015, Lőw et al., 2013, Tang et al., 2013)

involved_in cellular response to oxidative stress

inferred from mutant phenotype

(Tang et al., 2013)

involved_in cellular response to starvation

inferred from mutant phenotype

(Tang et al., 2013, Zirin et al., 2013)

involved_in glycophagy

inferred from mutant phenotype

(Zirin et al., 2013)

involved_in intestinal epithelial structure maintenance

inferred from mutant phenotype

(Wen et al., 2017)

involved_in larval midgut cell programmed cell death

inferred from mutant phenotype

(Xu et al., 2015)

involved_in larval midgut histolysis

inferred from mutant phenotype

(Wen et al., 2017)

involved_in negative regulation of TOR signaling

inferred from mutant phenotype

(Wen et al., 2017)

involved_in positive regulation of JNK cascade

inferred from mutant phenotype

(Tang et al., 2013)

inferred from genetic interaction with FLYBASE:bsk; FB:FBgn0000229

(Tang et al., 2013)

inferred from genetic interaction with FLYBASE:puc; FB:FBgn0243512

(Tang et al., 2013)

Terms Based on Predictions or Assertions (4 terms)

CV Term

Evidence

References

involved_in autophagosome assembly

inferred from sequence or structural similarity with SGD:S000002308

(FlyBase Curators, 2008--)

involved_in autophagy of mitochondrion

inferred from biological aspect of ancestor with PANTHER:PTN000315580

(assigned by GO_Central )

(Gaudet et al., 2011)

involved_in late nucleophagy

inferred from biological aspect of ancestor with PANTHER:PTN000315580

(assigned by GO_Central )

(Gaudet et al., 2011)

involved_in protein localization to phagophore assembly site

inferred from biological aspect of ancestor with PANTHER:PTN000315580

(assigned by GO_Central )

(Gaudet et al., 2011)

Cellular Component (5 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

located_in cell junction

inferred from direct assay

(Tang et al., 2013)

located_in cytoplasm

inferred from direct assay

(Tang et al., 2013)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

is_active_in autophagosome

inferred from biological aspect of ancestor with PANTHER:PTN000315580

(assigned by GO_Central )

(Gaudet et al., 2011)

located_in integral component of membrane

inferred from sequence model

(FlyBase Curators, 2011-)

is_active_in phagophore assembly site

inferred from biological aspect of ancestor with PANTHER:PTN000315580

(assigned by GO_Central )

(Gaudet et al., 2011)

Gene Group (FlyBase)

AUTOPHAGY-RELATED GENES

Protein Family (UniProt)

-

Protein Signatures (InterPro)

Autophagy-related protein 9

Summaries

Gene Snapshot

Autophagy-related 9 (Atg9) encodes an integral membrane protein acting as a membrane carrier for forming autophagosomes. It regulates TOR and JNK signaling pathways via autophagy. [Date last reviewed: 2019-07-11]

Gene Group (FlyBase)

AUTOPHAGY-RELATED GENES -: The Autophagy-related genes (Atg), characterized extensively in yeast, are a set of approximately twenty evolutionarily conserved genes required for autophagy. Autophagy (specifically, macroautophagy) is the process of bulk degradation of cytoplasmic material. It takes place by the assembly of a double membrane vesicle, the autophagosome, which delivers cargo to the lysosome for recycling. (Adapted from FBrf0219844 and FBrf020902).

Summary (Interactive Fly)

Interactive Fly

Gene Model and Products

Number of Transcripts

2

Number of Unique Polypeptides

2

Please see the JBrowse view of Dmel\Atg9 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Comments on Gene Model

Stop-codon suppression (UAG) postulated; FBrf0216884

Gene model reviewed during 5.44

Gene model reviewed during 5.50

Sequence Ontology: Class of Gene

gene_with_stop_codon_read_through

(Jungreis et al., 2011)

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

FBtr0087141

2833

845

FBtr0329958

2833

852

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

FBpp0086285

95.4

845

6.66

FBpp0302990

96.3

852

6.65

Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Autophagy-related protein 9

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Atg9 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Atg9_1

Linkouts

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 7 -- 8

antennal anlage

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)

central brain anlage

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)

dorsal head epidermis anlage

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 9 -- 10

organism | faint | ubiquitous

(Fisher et al., 2012)

embryonic stage 11 -- 12

organism | faint | ubiquitous

(Fisher et al., 2012)

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

(Lee et al., 2003)

Additional Descriptive Data

The expression of this gene along with several others is induced in salivary glands in pupae at the time of head eversion. This stage is characterized by an increase in the ecdysone titer as well as large amounts of cell death in this tissue.

(Lee et al., 2003)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

• membrane

(Aradska et al., 2015)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in cell junction

inferred from direct assay

(Tang et al., 2013)

located_in cytoplasm

inferred from direct assay

(Tang et al., 2013)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Atg9 in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Atg9

View exonic expression by tissue for Dmel\Atg9

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0034110

DRscDB

36821/tissue=All

EMBL-EBI Single Cell Expression Atlas

FBgn0034110

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG3615-RA

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG3615

Flygut - An atlas of the Drosophila adult midgut

FBgn0034110

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\Atg9

Stages(s) 1-3
Stages(s) 4-6
Stages(s) 7-8
Stages(s) 9-10
Stages(s) 11-12

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 4 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 14 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Atg9

Transgenic constructs containing regulatory region of Atg9

Aberrations (Deficiencies and Duplications) ( 2 )

Inferred from experimentation ( 2 )

Gene disrupted in

(Ryder, 2004.4.26)

(Ryder, 2004.4.26)

Inferred from location ( 0 )

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

Atg9^Gal4KO

lethal (with Atg9^d51)

Atg9^Gal4KO

lethal (with Atg9^Gal4KO)

Atg9^d51

lethal | recessive

Atg9^d51

partially lethal

Atg9^B5

partially lethal (with Df(2R)ED2487)

Atg9^B5

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Atg9^GD2029

viable, with Scer\GAL4^Mef2.PR

Atg9^GD2029

viable, with Scer\GAL4^pnr-MD237

Atg9^GD2029

viable, with Scer\GAL4^tin.CΔ4

Atg9^GD2029

Sterility

Allele

female semi-sterile

Atg9^B5

Atg9^B5

sterile (with Atg9^d51)

Atg9^Gal4KO

sterile (with Atg9^Gal4KO)

Atg9^d51

Other Phenotypes

Allele

abnormal cell shape, with Scer\GAL4^NP0001

Atg9^HMS01246

abnormal cell shape | adult stage, with Scer\GAL4^Tub.PU

Atg9^HMS01246

abnormal cell shape | adult stage (with Atg9^d51)

Atg9^Gal4KO

abnormal cell shape | adult stage (with Atg9^Gal4KO)

Atg9^d51

abnormal locomotor behavior | adult stage (with Atg9^d51)

Atg9^Gal4KO

abnormal locomotor behavior | adult stage (with Atg9^Gal4KO)

Atg9^d51

abnormal size | adult stage (with Atg9^d51)

Atg9^Gal4KO

abnormal size | adult stage (with Atg9^Gal4KO)

Atg9^d51

abnormal size | larval stage (with Atg9^d51)

Atg9^Gal4KO

abnormal size | larval stage (with Atg9^Gal4KO)

Atg9^d51

abnormal size | P-stage, with Scer\GAL4^NP0001

Atg9^JF02891

abnormal starvation stress response | larval stage, with Scer\GAL4^Cg.PU

Atg9^JF02891

decreased cell death | P-stage, with Scer\GAL4^NP0001

Atg9^JF02891

decreased fecundity | female

Atg9^B5

increased cell death | third instar larval stage, with Scer\GAL4^GMR.PU

Atg9^UAS.Tag:FLAG

increased cell size, with Scer\GAL4^NP0001

Atg9^HMS01246

increased cell size | adult stage, with Scer\GAL4^Tub.PU

Atg9^HMS01246

increased cell size | adult stage (with Atg9^d51)

Atg9^Gal4KO

increased cell size | adult stage (with Atg9^Gal4KO)

Atg9^d51

increased cell size | adult stage | somatic clone

Atg9^d51

increased occurrence of cell division | adult stage, with Scer\GAL4^esg.PU

Atg9^HMS01246

short lived, with Scer\GAL4^Mef2.PU

Atg9^GD2029

short lived, with Scer\GAL4^tin.CΔ4

Atg9^GD2029

short lived, with Scer\GAL4^Tub.PU

Atg9^HMS01246

short lived (with Atg9^d51)

Atg9^Gal4KO

short lived (with Atg9^Gal4KO)

Atg9^d51

visible | adult stage, with Scer\GAL4^ap-md544

Atg9^VSH330100

visible | adult stage, with Scer\GAL4^GMR.PU

Atg9^UAS.Tag:FLAG

Phenotype manifest in

Allele

actin filament bundle | female

Atg9^B5

adult fat body (with Atg9^d51)

Atg9^Gal4KO

adult fat body (with Atg9^Gal4KO)

Atg9^d51

adult fat body | nutrition conditional, with Scer\GAL4^Tub.PU

Atg9^HMS01246

adult heart muscle | progressive, with Scer\GAL4^Mef2.PU

Atg9^GD2029

adult midgut (with Atg9^d51)

Atg9^Gal4KO

adult midgut (with Atg9^Gal4KO)

Atg9^d51

adult midgut epithelium, with Scer\GAL4^esg.PU

Atg9^HMS01246

adult midgut epithelium, with Scer\GAL4^NP0001

Atg9^HMS01246

adult midgut epithelium, with Scer\GAL4^Tub.PU

Atg9^HMS01246

adult midgut epithelium (with Atg9^d51)

Atg9^Gal4KO

adult midgut epithelium (with Atg9^Gal4KO)

Atg9^d51

autophagosome | :chemical conditional | nutrition conditional, with Scer\GAL4^Mef2.PR

Atg9^JF02891

autophagosome | adult stage | nutrition conditional, with Scer\GAL4^Tub.PU

Atg9^HMS01246

autophagosome | adult stage | nutrition conditional (with Atg9^d51)

Atg9^Gal4KO

autophagosome | adult stage | nutrition conditional (with Atg9^Gal4KO)

Atg9^d51

autophagosome | larval stage (with Atg9^d51)

Atg9^Gal4KO

autophagosome | larval stage (with Atg9^Gal4KO)

Atg9^d51

axon | embryonic stage

Atg9^B5

cytoplasmic vesicle | early third instar larval stage, with Scer\GAL4^Cg.PA

Atg9^HMS01246

Atg9^B5

egg | germline clone

Atg9^B5

embryonic/larval fat body | larval stage, with Scer\GAL4^Cg.PU

Atg9^JF02891

embryonic/larval fat body | larval stage (with Atg9^d51)

Atg9^Gal4KO

embryonic/larval fat body | larval stage (with Atg9^Gal4KO)

Atg9^d51

embryonic/larval fat body | somatic clone | third instar larval stage, with Scer\GAL4^Act5C.PP

Atg9^JF02891

embryonic/larval midgut | P-stage, with Scer\GAL4^NP0001

Atg9^JF02891

enterocyte of posterior adult midgut epithelium | somatic clone

Atg9^d51

eye, with Scer\GAL4^GMR.PU

Atg9^UAS.Tag:FLAG

eye disc | third instar larval stage, with Scer\GAL4^GMR.PU

Atg9^UAS.Tag:FLAG

fat cell | early third instar larval stage | somatic clone, with Scer\GAL4^Cg.PA

Atg9^HMS01246

filopodium | increased number

Atg9^B5

gastric caecum (with Atg9^d51)

Atg9^Gal4KO

gastric caecum (with Atg9^Gal4KO)

Atg9^d51

gastric caecum | P-stage, with Scer\GAL4^NP0001

Atg9^JF02891

indirect flight muscle | day 40 of adulthood, with Scer\GAL4^Mef2.PU

Atg9^GD2029

intestinal stem cell | chemical conditional, with Scer\GAL4^NP0001

Atg9^GD2029

intestinal stem cell | chemical conditional, with Scer\GAL4^NP5130

Atg9^GD2029

larval ventral nerve cord | embryonic stage

Atg9^B5

lysosome | adult stage | nutrition conditional, with Scer\GAL4^Tub.PU

Atg9^HMS01246

lysosome | adult stage | nutrition conditional (with Atg9^d51)

Atg9^Gal4KO

lysosome | adult stage | nutrition conditional (with Atg9^Gal4KO)

Atg9^d51

lysosome | larval stage (with Atg9^d51)

Atg9^Gal4KO

lysosome | larval stage (with Atg9^Gal4KO)

Atg9^d51

mitochondrion | adult stage, with Scer\GAL4^Mef2.PU

Atg9^GD2029

nucleus | female

Atg9^B5

Atg9^B5

nurse cell | germline clone

Atg9^B5

skeletal muscle of thorax | adult stage (with Atg9^d51)

Atg9^Gal4KO

skeletal muscle of thorax | adult stage (with Atg9^Gal4KO)

Atg9^d51

visceral mesoderm | adult stage (with Atg9^d51)

Atg9^Gal4KO

visceral mesoderm | adult stage (with Atg9^Gal4KO)

Atg9^d51

wing, with Scer\GAL4^ap-md544

Atg9^VSH330100

Orthologs

Downloads

Download All DIOPT Orthologs

Download All OrthoDB Orthologs

Human Orthologs (via DIOPT v8.0)

Homo sapiens (Human) (4)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

12 of 15

Yes

Yes

1

6 of 15

No

Yes

2 of 15

No

No

1 of 15

No

No

Model Organism Orthologs (via DIOPT v8.0)

Mus musculus (laboratory mouse) (2)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

10 of 15

Yes

Yes

10 of 15

Yes

Yes

Rattus norvegicus (Norway rat) (3)

11 of 13

Yes

Yes

10 of 13

No

Yes

Rnor\LOC102557368

1 of 13

No

Yes

Xenopus tropicalis (Western clawed frog) (2)

9 of 12

Yes

Yes

8 of 12

No

Yes

Danio rerio (Zebrafish) (2)

12 of 15

Yes

Yes

7 of 15

No

Yes

Caenorhabditis elegans (Nematode, roundworm) (1)

13 of 15

Yes

Yes

Arabidopsis thaliana (thale-cress) (1)

8 of 9

Yes

Yes

Saccharomyces cerevisiae (Brewer's yeast) (1)

11 of 15

Yes

Yes

Schizosaccharomyces pombe (Fission yeast) (1)

9 of 12

Yes

Yes

Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG091902Z2 )

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila suzukii

Spotted wing Drosophila

Dsuz\DS10_00003189

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Drosophila persimilis

Drosophila willistoni

Drosophila virilis

Drosophila mojavensis

Drosophila grimshawi

Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091501UT )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Musca domestica

House fly

Glossina morsitans

Tsetse fly

Gmor\GMOY009568

Lucilia cuprina

Australian sheep blowfly

Lcup\FF38_02719

Mayetiola destructor

Hessian fly

Mdes\Mdes002285

Aedes aegypti

Yellow fever mosquito

Anopheles darlingi

American malaria mosquito

Adar\ADAC010428

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ012964

Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W02WN )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Bombyx mori

Silkmoth

Bmor\BGIBMGA012307

Danaus plexippus

Monarch butterfly

Dple\DPOGS212572-PA

Heliconius melpomene

Postman butterfly

Hmel\HMEL008818

Apis florea

Little honeybee

Aflo\2998971332

Apis mellifera

Western honey bee

Bombus impatiens

Common eastern bumble bee

Bombus terrestris

Buff-tailed bumblebee

Linepithema humile

Argentine ant

Megachile rotundata

Alfalfa leafcutting bee

Mrot\MROTU:00095a

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG037281

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG021819

Dendroctonus ponderosae

Mountain pine beetle

Tribolium castaneum

Red flour beetle

Pediculus humanus

Human body louse

Phum\PHUM577780

Rhodnius prolixus

Kissing bug

Rpro\RPRC003473

Cimex lectularius

Bed bug

Clec\CLEC011740

Cimex lectularius

Bed bug

Clec\CLEC011737

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI000660

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI067571

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_01174

Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X04CB )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strigamia maritima

European centipede

Smar\SMAR011766

Stegodyphus mimosarum

African social velvet spider

Smim\KFM71307.1

Stegodyphus mimosarum

African social velvet spider

Smim\KFM71308.1

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_41567

Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G01WQ )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strongylocentrotus purpuratus

Purple sea urchin

Strongylocentrotus purpuratus

Purple sea urchin

Spur\SPU_018436gn

Strongylocentrotus purpuratus

Purple sea urchin

Spur\SPU_014942gn

Ciona intestinalis

Vase tunicate

Gallus gallus

Domestic chicken

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v8.0)

Drosophila melanogaster (Fruit fly) (0)

No records found.

Human Disease Associations

FlyBase Human Disease Model Reports

cancer, epithelial, RAS models, role of autophagy

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Allele

Disease

Interaction

References

ameliorates myotonic dystrophy type 1

modeled by Zzzz\CTG^i480.UAS.cGa

(Bargiela et al., 2015)

Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

ATG9A; autophagy related 9A

12 of 15

ATG9B; autophagy related 9B

6 of 15

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

View in Interactions Browser

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

anti tag coimmunoprecipitation, anti tag western blot

(Nagy et al., 2014)

experimental knowledge based

(Guruharsha et al., 2011)

anti tag coimmunoprecipitation, western blot

(Soukup et al., 2016)

anti tag coimmunoprecipitation, anti tag western blot, pull down, western blot

(Wen et al., 2017)

anti bait coimmunoprecipitation, anti tag western blot, pull down, anti tag coimmunoprecipitation, western blot

(Tang et al., 2013)

two hybrid, anti tag coimmunoprecipitation, western blot, pull down, anti tag western blot

(Kiss et al., 2020)

(Kiss et al., 2020)

anti tag coimmunoprecipitation, anti tag western blot

(Wen et al., 2017)

anti tag coimmunoprecipitation, anti tag western blot

(Nagy et al., 2014)

Summary of Genetic Interactions

esyN Network Diagram

esyN Network Key:

Suppression

Enhancement

View in Interactions Browser

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

suppressible

(Wen et al., 2017)

enhanceable

(Wen et al., 2017)

suppressible

(Wen et al., 2017)

suppressible

(Tang et al., 2013)

suppressible

(Tang et al., 2013)

enhanceable

(Kiss et al., 2020)

enhanceable

(Kiss et al., 2020)

enhanceable

(Kiss et al., 2020)

suppressible

(Wen et al., 2017)

suppressible

(Wen et al., 2017)

suppressible

(Wen et al., 2017)

suppressible

(Tang et al., 2013)

suppressible

(Wen et al., 2017)

suppressible

(Wen et al., 2017)

suppressible

(Tang et al., 2013)

suppressible

(Wen et al., 2017)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

suppressible

(Tang et al., 2018)

suppressible

(Tang et al., 2013)

suppressible

(Kiss et al., 2020)

suppressible

(Shinoda et al., 2019)

suppressible

(Shinoda et al., 2019)

enhanceable

(Kwon et al., 2013)

suppressible

(Tang et al., 2013)

suppressible

(Liu et al., 2018)

External Data

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0034110

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

36821

MIST (genetic) - An integrated Molecular Interaction Database

Atg9

MIST (protein-protein) - An integrated Molecular Interaction Database

Atg9

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

External Data

Linkouts

KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Macroautophagy

Genomic Location and Detailed Mapping Data

Chromosome (arm)

2R

Recombination map

2-79

Cytogenetic map

Sequence location

2R:16,294,400..16,297,232 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

53B1-53B2

Limits computationally determined from genome sequence between P{lacW}Lis-1^k11702&P{EP}CG8443^EP969 and P{lacW}l(2)k07824^k07824&P{lacW}veg^k03402

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

2-79

(FlyBase, 2016)

2-81.9

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (5)

28055

y¹ v¹; P{TRiP.JF02891}attP2

61054

y¹ w^*; Mi{MIC}Atg9^MI15447

34901

y¹ sc^* v¹ sev²¹; P{TRiP.HMS01246}attP2

v330100

P{VSH330100}attP40

v10045

w¹¹¹⁸; P{GD2029}v10045

Genomic Clones (18)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (201)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

DPiM_RE14003

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0034110

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0034110

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

36821

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Nagy et al., 2014, Tang et al., 2013)

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for identity of: Atg9 CG3615

(Scott et al., 2004)

Source for database merge of

Additional comments

Other Comments

Origin and Etymology

Discoverer

Etymology

Identification

External Crossreferences and Linkouts ( 45 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

36821

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0034110

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0034110

DRscDB

36821/tissue=All

EMBL-EBI Single Cell Expression Atlas

FBgn0034110

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Atg9_1

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG3615

Flygut - An atlas of the Drosophila adult midgut

FBgn0034110

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

36821

InterPro - A database of protein families, domains and functional sites

Autophagy-related protein 9

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG3615

MARRVEL_MODEL

36821

modMine - A data warehouse for the modENCODE project

FBgn0034110

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0034110

DRSC - Results frm RNAi screens

FBgn0034110

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG3615-RA

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0034110

FlyMine - An integrated database for Drosophila genomics

FBgn0034110

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

36821

KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.

MIST (genetic) - An integrated Molecular Interaction Database

Atg9

MIST (protein-protein) - An integrated Molecular Interaction Database

Atg9

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Macroautophagy

Synonyms and Secondary IDs (7)

Reported As

Symbol Synonym

ATG9

(Song et al., 2020, Yang et al., 2020, Kourtis and Tavernarakis, 2009)

Atg9

(Allen et al., 2020, Barthez et al., 2020, Chittoor-Vinod et al., 2020, Cunningham et al., 2020, Jacomin et al., 2020, Kiss et al., 2020, Khezri and Rusten, 2019, Meltzer et al., 2019, Prasad et al., 2019, Texada et al., 2019, Xu et al., 2019, Yan et al., 2019, Zhang et al., 2019, Billes et al., 2018, Csizmadia et al., 2018, Kuo et al., 2018, Liu et al., 2018, O'Keefe and Denton, 2018, Richardson and Portela, 2018, Tang et al., 2018, Bali and Shravage, 2017, Galluzzi et al., 2017, Kim et al., 2017, Kuleesha et al., 2017, Manent et al., 2017, Péan et al., 2017, Wen et al., 2017, Kuleesha et al., 2016, Soukup et al., 2016, Bargiela et al., 2015, Denton et al., 2015, Kim et al., 2015, Xu et al., 2015, Yadav et al., 2015, Zhang and Baehrecke, 2015, Zirin et al., 2015, Banreti et al., 2014, Mulakkal et al., 2014, Nagy et al., 2014, Denton et al., 2013, Kwon et al., 2013, Lőw et al., 2013, Nagy et al., 2013, Tang et al., 2013, Velentzas et al., 2013, Zirin et al., 2013, Érdi et al., 2012, Garcia et al., 2012, Japanese National Institute of Genetics, 2012.5.21, Pircs et al., 2012, Jungreis et al., 2011, Qin et al., 2011, Chang and Neufeld, 2010, Kadandale et al., 2010, Liu and Lehmann, 2008, Martin et al., 2007)

CG3615

(Meadows, 2017.1.11, Fukuoh et al., 2014, Érdi et al., 2012, Ni et al., 2011.7.19, Perkins et al., 2009, Dostert et al., 2005, Lee et al., 2003, Boutros et al., 2002)

atg9

(Kinser and Pincus, 2020, Bhukel et al., 2019, Shinoda et al., 2019, Singh and Chowdhuri, 2018, Zhang et al., 2016, Kwon et al., 2013, Chen et al., 2012, Shelly et al., 2009)

dAtg9

(Li et al., 2016)

Name Synonyms

Autophagy-related 9

Autophagy-specific gene 9

(Scott et al., 2004)

Secondary FlyBase IDs

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

References (122)

Report Sections