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General Information
Symbol
Dmel\DAT
Species
D. melanogaster
Name
Dopamine transporter
Annotation Symbol
CG8380
Feature Type
FlyBase ID
FBgn0034136
Gene Model Status
Stock Availability
Gene Summary
Dopamine transporter (DAT) encodes a dopamine transporter that mediates uptake of dopamine from the synaptic cleft. Its loss increases extracellular dopamine and is associated with behavioral phenotypes including increased activity and decreased sleep. [Date last reviewed: 2019-03-07] (FlyBase Gene Snapshot)
Also Known As

fumin, fmn, dDAT, DmDAT

Key Links
Genomic Location
Cytogenetic map
Sequence location
Recombination map
2-80
RefSeq locus
NT_033778 REGION:16558567..16565238
Sequence
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
Function
GO Summary Ribbons
Gene Ontology (GO) Annotations (15 terms)
Molecular Function (2 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
inferred from direct assay
inferred from direct assay
inferred from direct assay
(assigned by UniProt )
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
inferred from biological aspect of ancestor with PANTHER:PTN000746001
(assigned by GO_Central )
Biological Process (9 terms)
Terms Based on Experimental Evidence (6 terms)
CV Term
Evidence
References
inferred from mutant phenotype
inferred from mutant phenotype
(assigned by UniProt )
inferred from direct assay
involved_in dopamine transport
inferred from direct assay
(assigned by UniProt )
inferred from direct assay
inferred from mutant phenotype
involved_in sleep
inferred from mutant phenotype
Terms Based on Predictions or Assertions (3 terms)
CV Term
Evidence
References
inferred from biological aspect of ancestor with PANTHER:PTN000746001
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN000746001
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN000177662
(assigned by GO_Central )
Cellular Component (4 terms)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
inferred from direct assay
(assigned by UniProt )
Terms Based on Predictions or Assertions (4 terms)
CV Term
Evidence
References
inferred from biological aspect of ancestor with PANTHER:PTN000177662
(assigned by GO_Central )
inferred from sequence model
is_active_in neuron projection
inferred from biological aspect of ancestor with PANTHER:PTN000746001
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN000746001
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN000746001
(assigned by GO_Central )
Protein Family (UniProt)
Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. (Q7K4Y6)
Summaries
Gene Snapshot
Dopamine transporter (DAT) encodes a dopamine transporter that mediates uptake of dopamine from the synaptic cleft. Its loss increases extracellular dopamine and is associated with behavioral phenotypes including increased activity and decreased sleep. [Date last reviewed: 2019-03-07]
Gene Group (FlyBase)
SLC6 FAMILY OF SODIUM/CHLORIDE-DEPENDENT NEUROTRANSMITTER TRANSPORTERS -
Solute carrier family 6 (SLC6) members are Na+/Cl--dependent transporters of extracellular neurotransmitters, amino acids and osmolytes across the plasma membrane into the cytosol. (Adapted from FBrf0193583).
Protein Function (UniProtKB)
Sodium-dependent dopamine transporter which terminates the action of dopamine by its high affinity sodium-dependent reuptake into presynaptic terminals (PubMed:11125028, PubMed:12606774, PubMed:24037379, PubMed:25970245). Also transports tyramine and norepinephrine, shows less efficient transport of octopamine and does not transport serotonin (PubMed:11125028, PubMed:12606774). Plays a role in the regulation of the rest/activity cycle (PubMed:16093388, PubMed:25232310).
(UniProt, Q7K4Y6)
Summary (Interactive Fly)
Gene Model and Products
Number of Transcripts
2
Number of Unique Polypeptides
2

Please see the JBrowse view of Dmel\DAT for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Structure New Section
Protein 3D structure   (Predicted by AlphaFold)   (AlphaFold entry Q7K4Y6)

If you don't see the viewer to the right, refresh your browser.
Model Confidence:
  • Very high (pLDDT > 90)
  • Confident (90 > pLDDT > 70)
  • Low (70 > pLDDT > 50)
  • Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Comments on Gene Model

Stop-codon suppression (UGA) postulated; FBrf0216884

Gene model reviewed during 5.44

Gene model reviewed during 5.50

Sequence Ontology: Class of Gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
FBtr0087125
3040
631
FBtr0330002
3040
644
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
FBpp0086271
70.3
631
6.31
FBpp0303037
71.9
644
6.70
Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\DAT using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
posterior ectoderm anlage

Comment: anlage in statu nascendi

antennal anlage in statu nascendi

Comment: reported as procephalic ectoderm anlage in statu nascendi

dorsal head epidermis anlage in statu nascendi

Comment: reported as procephalic ectoderm anlage in statu nascendi

visual anlage in statu nascendi

Comment: reported as procephalic ectoderm anlage in statu nascendi

northern blot
Stage
Tissue/Position (including subcellular localization)
Reference
adult thorax

Comment: Thorax and abdomen assayed together.

adult abdomen

Comment: Thorax and abdomen assayed together.

RT-PCR
Stage
Tissue/Position (including subcellular localization)
Reference
organism | faint

Comment: residual maternal transcript

Comment: 12-24 hr AEL; not expressed 4-12 hr AEL

Additional Descriptive Data

In situ hybridization in third instar larvae show that DAT is expressed in dopaminergic (DA) neurons. In the brain, it is observed in three DA neuron cell groups in each lobe. In the ventral nerve cord, it is seen in eight unpaired medial DA neurons along the midline and in two rows of seven dorsal lateral DA neurons. A developmental profile done by RT-PCR show robust expression in late embryos and larvae, a dip in expression in pupae and an increase in adults, with heads showing enhanced signal over bodies.

Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
inferred from direct assay
(assigned by UniProt )
Expression Deduced from Reporters
Reporter: P{DAT-B-GAL4}
Stage
Tissue/Position (including subcellular localization)
Reference
Reporter: P{DAT-GAL4.L}
Stage
Tissue/Position (including subcellular localization)
Reference
Reporter: P{GMR55C10-GAL4}
Stage
Tissue/Position (including subcellular localization)
Reference
Reporter: P{GMR58E02-GAL4}
Stage
Tissue/Position (including subcellular localization)
Reference
Reporter: P{GMR58E02-lexA}
Stage
Tissue/Position (including subcellular localization)
Reference
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\DAT in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyExpress - Embryonic expression images (BDGP data)
  • Stages(s) 4-6
  • Stages(s) 7-8
  • Stages(s) 13-16
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 9 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 10 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of DAT
Transgenic constructs containing regulatory region of DAT
Aberrations (Deficiencies and Duplications) ( 3 )
Inferred from experimentation ( 3 )
Gene not disrupted in
Inferred from location ( 0 )
Alleles Representing Disease-Implicated Variants
Phenotypes
Orthologs
Human Orthologs (via DIOPT v8.0)
Homo sapiens (Human) (19)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
14 of 15
Yes
Yes
10 of 15
No
Yes
 
19  
5 of 15
No
No
1  
3 of 15
No
No
3 of 15
No
No
3 of 15
No
No
3 of 15
No
No
3 of 15
No
No
3 of 15
No
No
3 of 15
No
No
3 of 15
No
No
3 of 15
No
No
3 of 15
No
No
3 of 15
No
No
2 of 15
No
No
1  
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
1  
2 of 15
No
No
Model Organism Orthologs (via DIOPT v8.0)
Mus musculus (laboratory mouse) (22)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
13 of 15
Yes
Yes
10 of 15
No
Yes
5 of 15
No
No
3 of 15
No
No
3 of 15
No
No
3 of 15
No
No
3 of 15
No
No
3 of 15
No
No
3 of 15
No
No
3 of 15
No
No
3 of 15
No
No
3 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
1 of 15
No
Yes
Rattus norvegicus (Norway rat) (21)
9 of 13
Yes
Yes
8 of 13
No
Yes
5 of 13
No
No
3 of 13
No
No
3 of 13
No
No
3 of 13
No
No
3 of 13
No
No
3 of 13
No
No
3 of 13
No
No
3 of 13
No
No
3 of 13
No
No
3 of 13
No
No
3 of 13
No
No
2 of 13
No
No
2 of 13
No
No
2 of 13
No
No
2 of 13
No
No
2 of 13
No
No
2 of 13
No
No
2 of 13
No
No
1 of 13
No
Yes
Xenopus tropicalis (Western clawed frog) (23)
8 of 12
Yes
Yes
6 of 12
No
Yes
4 of 12
No
No
3 of 12
No
No
3 of 12
No
No
3 of 12
No
No
3 of 12
No
No
3 of 12
No
No
3 of 12
No
No
3 of 12
No
No
3 of 12
No
Yes
3 of 12
No
No
3 of 12
No
No
3 of 12
No
No
2 of 12
No
Yes
2 of 12
No
No
2 of 12
No
No
2 of 12
No
No
2 of 12
No
No
1 of 12
No
Yes
1 of 12
No
Yes
1 of 12
No
Yes
1 of 12
No
Yes
Danio rerio (Zebrafish) (36)
13 of 15
Yes
Yes
5 of 15
No
No
5 of 15
No
No
4 of 15
No
Yes
3 of 15
No
No
3 of 15
No
No
3 of 15
No
No
3 of 15
No
No
3 of 15
No
No
3 of 15
No
No
3 of 15
No
No
3 of 15
No
No
3 of 15
No
No
3 of 15
No
No
3 of 15
No
No
3 of 15
No
No
3 of 15
No
No
3 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
1 of 15
No
No
Caenorhabditis elegans (Nematode, roundworm) (16)
15 of 15
Yes
Yes
4 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
Arabidopsis thaliana (thale-cress) (0)
No records found.
Saccharomyces cerevisiae (Brewer's yeast) (0)
No records found.
Schizosaccharomyces pombe (Fission yeast) (0)
No records found.
Other Organism Orthologs (via OrthoDB)
Paralogs
Paralogs (via DIOPT v8.0)
Drosophila melanogaster (Fruit fly) (22)
6 of 10
4 of 10
4 of 10
4 of 10
4 of 10
4 of 10
4 of 10
4 of 10
4 of 10
4 of 10
3 of 10
3 of 10
3 of 10
3 of 10
3 of 10
3 of 10
3 of 10
2 of 10
2 of 10
2 of 10
2 of 10
1 of 10
Human Disease Associations
FlyBase Human Disease Model Reports
Disease Model Summary Ribbon
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 2 )
Potential Models Based on Orthology ( 1 )
Modifiers Based on Experimental Evidence ( 1 )
Allele
Disease
Interaction
References
Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)
Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
Functional Complementation Data
Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
Dmel gene
Ortholog showing functional complementation
Supporting References
Interactions
Summary of Physical Interactions
esyN Network Diagram
Interactions Browser
Summary of Genetic Interactions
esyN Network Diagram
Starting gene(s)
Interaction type
Interacting gene(s)
Reference
Starting gene(s)
Interaction type
Interacting gene(s)
Reference
External Data
Linkouts
DroID - A comprehensive database of gene and protein interactions.
Pathways
Signaling Pathways (FlyBase)
Metabolic Pathways
FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.
External Data
Linkouts
Genomic Location and Detailed Mapping Data
Chromosome (arm)
2R
Recombination map
2-80
Cytogenetic map
Sequence location
FlyBase Computed Cytological Location
Cytogenetic map
Evidence for location
53C7-53C8
Limits computationally determined from genome sequence between P{PZ}Sema-2a03021&P{PZ}Cdk405428 and P{EP}BEST:SD02913EP2148&P{lacW}Dekk09907
Experimentally Determined Cytological Location
Cytogenetic map
Notes
References
53C7-53C14
Experimentally Determined Recombination Data
Left of (cM)
Notes
Stocks and Reagents
Stocks (15)
Genomic Clones (20)
cDNA Clones (7)
 

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced
BDGP DGC clones
Other clones
    Drosophila Genomics Resource Center cDNA clones

    For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

    cDNA Clones, End Sequenced (ESTs)
    BDGP DGC clones
    RNAi and Array Information
    Linkouts
    DRSC - Results frm RNAi screens
    GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
    Antibody Information
    Laboratory Generated Antibodies
    Commercially Available Antibodies
     
    Other Information
    Relationship to Other Genes
    Source for database identify of
    Source for database merge of

    Source for merge of: DAT CG8380

    Additional comments

    Source for merge of DAT CG8380 was sequence comparison ( date:000622 ).

    Other Comments

    The elimination of DAT activity generates a dramatic increase in the length of the active (waking) phase and a corresponding reduction of the inactive phase (sleep or rest), resulting a a nearly sleepless phenotype.

    Origin and Etymology
    Discoverer
    Etymology

    Called 'fumin' (fmn), meaning 'sleepless' in Japanese.

    Identification
    External Crossreferences and Linkouts ( 43 )
    Sequence Crossreferences
    NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
    GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
    GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
    RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
    UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.
    UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
    UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
    Other crossreferences
    AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.
    BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
    Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
    DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
    EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
    Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
    FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
    FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
    Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
    Flygut - An atlas of the Drosophila adult midgut
    FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.
    GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
    iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
    InterPro - A database of protein families, domains and functional sites
    KEGG Genes - Molecular building blocks of life in the genomic space.
    MARRVEL_MODEL - MARRVEL (model organism gene)
    modMine - A data warehouse for the modENCODE project
    Linkouts
    DroID - A comprehensive database of gene and protein interactions.
    DRSC - Results frm RNAi screens
    FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
    FlyMine - An integrated database for Drosophila genomics
    Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
    Synonyms and Secondary IDs (11)
    Reported As
    Symbol Synonym
    Secondary FlyBase IDs
      Datasets (7)
      Study focus (0)
      Experimental Role
      Project
      Project Type
      Title
      Study result (7)
      Result
      Result Type
      Title
      Clustering analysis of hemocytes from wasp-infested third instar larvae
      Clustering analysis of lymph gland cells from non-infested larvae at 72 h after egg-laying
      Clustering analysis of lymph gland cells from non-infested larvae at 96 h after egg-laying
      Clustering analysis of lymph gland cells from non-infested larvae at 120 h after egg-laying
      Clustering analysis of lymph gland cells from wasp-infested larvae at 96 h after egg-laying
      Clustering analysis of circulating hemocytes from non-infested larvae at 96 h after egg-laying
      Clustering analysis of circulating hemocytes from non-infested larvae at 120 h after egg-laying
      References (180)