FB2026_02 , released June 18, 2026
Gene: Dmel\maf-S
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General Information
Symbol
Dmel\maf-S
Species
D. melanogaster
Name
maf-S
Annotation Symbol
CG9954
Feature Type
protein_coding_gene
FlyBase ID
FBgn0034534
Gene Model Status
Current
Stock Availability
12 publicly available
Gene Summary
Contribute a Gene Snapshot for this gene.
All Summaries
Also Known As

maf2, Maf

Key Links
Genomic Location
Cytogenetic map
57A8-57A8
Sequence location
Recombination map
2-91
RefSeq locus
NT_033778 REGION:20640976..20641668
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
Gene Ontology (GO) Annotations (10 terms)
Molecular Function (5 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
contributes_to DNA-binding transcription factor activity
inferred from direct assay
(Veraksa et al., 2000)
enables protein heterodimerization activity
inferred from physical interaction with FLYBASE:cnc; FB:FBgn0262975
(Veraksa et al., 2000)
Terms Based on Predictions or Assertions (4 terms)
CV Term
Evidence
References
enables DNA binding
inferred from electronic annotation with InterPro:IPR004826, InterPro:IPR008917
(InterPro Project Members, 2004-)
enables DNA-binding transcription factor activity, RNA polymerase II-specific
inferred from biological aspect of ancestor with PANTHER:PTN002561999
(GO Reference Genome Project, 2011-)
enables DNA-binding transcription factor activity
inferred from electronic annotation with InterPro:IPR004827, InterPro:IPR024874
(InterPro Project Members, 2004-)
enables RNA polymerase II cis-regulatory region sequence-specific DNA binding
inferred from biological aspect of ancestor with PANTHER:PTN002561999
(GO Reference Genome Project, 2011-)
Biological Process (4 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
involved_in head development
inferred from mutant phenotype
(Veraksa et al., 2000)
involved_in positive regulation of transcription by RNA polymerase II
inferred from direct assay
(Veraksa et al., 2000)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
involved_in regulation of DNA-templated transcription
(InterPro Project Members, 2004-)
involved_in regulation of transcription by RNA polymerase II
inferred from biological aspect of ancestor with PANTHER:PTN002561999
(GO Reference Genome Project, 2011-)
Cellular Component (1 term)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
is_active_in nucleus
inferred from biological aspect of ancestor with PANTHER:PTN002561999
(GO Reference Genome Project, 2011-)
Gene Group (FlyBase)
Protein Family (UniProt)
-
Protein Signatures (InterPro)
Summaries
Gene Group (FlyBase)
BASIC LEUCINE ZIPPER TRANSCRIPTION FACTORS -
The basic leucine zipper (bZIP) transcription factors are sequence-specific DNA-binding proteins that regulate transcription. They are characterized by a 60-80 amino acid bZIP domain: a basic DNA binding domain followed by a leucine zipper dimerization domain. (Adapted from FBrf0152056).
Gene Model and Products
Number of Transcripts
2
Number of Unique Polypeptides
2

Please see the JBrowse view of Dmel\maf-S for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Structure
Protein 3D structure   (Predicted by AlphaFold)   (AlphaFold entry A0A0B4LH09)

If you don't see a structure in the viewer, refresh your browser.
Model Confidence:
  • Very high (pLDDT > 90)
  • Confident (90 > pLDDT > 70)
  • Low (70 > pLDDT > 50)
  • Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures
Crossreferences
Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.51

Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
maf-S-RA
FBtr0086230
NM_137656
631
132
maf-S-RB
FBtr0342716
NM_001299710
646
137
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
UniProt
RefSeq ID
GenBank
maf-S-PA
FBpp0085544
15.7
132
9.22
Q4QPP9
NP_611500
AAF57439
maf-S-PB
FBpp0309590
16.3
137
8.94
A0A0B4LH09
NP_001286639
AHN56434
Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\maf-S using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

1.07

Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage
organism | ubiquitous
(Veraksa et al., 2000)
embryonic stage 1 -- 3
organism

Comment: maternally deposited

(Fisher et al., 2012)
embryonic stage 4 -- 6
organism | ubiquitous
(Fisher et al., 2012)
embryonic stage 7 -- 8
organism | ubiquitous
(Fisher et al., 2012)
embryonic stage 8
cephalic furrow
(Veraksa et al., 2000)
mesoderm
(Veraksa et al., 2000)
embryonic stage 9 -- 10
anterior endoderm
(Fisher et al., 2012)
organism | faint | ubiquitous
(Fisher et al., 2012)
posterior endoderm
(Fisher et al., 2012)
trunk mesoderm
(Fisher et al., 2012)
embryonic stage 11 -- 12
anterior midgut primordium
(Fisher et al., 2012)
central brain primordium
(Fisher et al., 2012)
fat body/gonad primordium
(Fisher et al., 2012)
organism | faint | ubiquitous
(Fisher et al., 2012)
posterior midgut primordium
(Fisher et al., 2012)
ventral nerve cord primordium
(Fisher et al., 2012)
embryonic stage 11 -- 17
embryonic/larval midgut primordium
(Veraksa et al., 2000)
embryonic intercalary segment
(Veraksa et al., 2000)
presumptive embryonic/larval central nervous system
(Veraksa et al., 2000)
embryonic stage 13 -- 16
embryonic brain
(Fisher et al., 2012)
embryonic/larval midgut
(Fisher et al., 2012)
gonad
(Fisher et al., 2012)
organism | faint | ubiquitous
(Fisher et al., 2012)
larval ventral nerve cord
(Fisher et al., 2012)
Additional Descriptive Data

maf-S expression is ubiquitous in embryos at all stages, with enrichment in specific spatiotemporal areas. At embryonic stage 8, transcript levels are slightly higher in the mesoderm and the cephalic furrow than in the rest of the embryo. Starting at embryonic stage 11, expression is slightly enriched in the anterior and posterior midgut, in the central nervous system, and in the At stage 13, expression is slightly higher in the midgut, central nervous system, in the involuting intercalary segment that gives rise to the ventral pharynx.

(Veraksa et al., 2000)
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\maf-S in JBrowse
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 2 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 14 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of maf-S
Transgenic constructs containing regulatory region of maf-S
Aberrations (Deficiencies and Duplications) ( 3 )
Variants
Variant Molecular Consequences
Alleles Representing Disease-Implicated Variants
Phenotypes
Orthologs
Downloads
Download All DIOPT Orthologs
Human Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Homo sapiens (Human) (10)
Hsap\MAFK
13 of 14
Yes
Yes
1  
Hsap\MAFF
11 of 14
No
Yes
1  
Hsap\MAFG
11 of 14
No
Yes
1  
Hsap\MAFA
4 of 14
No
No
Hsap\MAFB
4 of 14
No
No
Hsap\MAF
4 of 14
No
No
Hsap\NRL
4 of 14
No
No
Hsap\HYDIN
1 of 14
No
No
Hsap\NFE2L1
1 of 14
No
No
Hsap\NFE2L2
1 of 14
No
No
Model Organism Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Rattus norvegicus (Norway rat) (9)
Rnor\Mafk
13 of 14
Yes
Yes
Rnor\Maff
11 of 14
No
Yes
Rnor\Mafg
11 of 14
No
Yes
Rnor\Mafa
4 of 14
No
No
Rnor\Mafb
4 of 14
No
No
Rnor\Maf
4 of 14
No
No
Rnor\Nrl
4 of 14
No
No
Rnor\Nfe2l1
1 of 14
No
No
Rnor\Nfe2l3
1 of 14
No
No
Mus musculus (laboratory mouse) (10)
Mmus\Mafk
13 of 14
Yes
Yes
Mmus\Mafg
11 of 14
No
Yes
Mmus\Maff
10 of 14
No
Yes
Mmus\Mafa
4 of 14
No
No
Mmus\Mafb
4 of 14
No
No
Mmus\Maf
4 of 14
No
No
Mmus\Nrl
4 of 14
No
No
Mmus\Nfe2l1
1 of 14
No
No
Mmus\Nfe2l3
1 of 14
No
No
Mmus\Spertl
1 of 14
No
Yes
Xenopus tropicalis (Western clawed frog) (13)
Xtro\mafk
7 of 13
Yes
Yes
Xtro\maff
6 of 13
No
Yes
Xtro\mafb
3 of 13
No
No
Xtro\mafa
2 of 13
No
No
Xtro\maf
2 of 13
No
No
Xtro\nrl
2 of 13
No
No
Xtro\mafg
1 of 13
No
Yes
Xtro\nfe2l1
1 of 13
No
No
Xtro\nfe2l3
1 of 13
No
No
Xtro\nfe2
1 of 13
No
No
Xtro\rab35
1 of 13
No
No
Xtro\tspan6
1 of 13
No
No
Xtro\xbp1
1 of 13
No
No
Danio rerio (Zebrafish) (14)
Drer\mafk
12 of 14
Yes
Yes
Drer\maff
11 of 14
No
Yes
Drer\mafga
10 of 14
No
Yes
Drer\mafgb
10 of 14
No
Yes
Drer\mafaa
4 of 14
No
No
Drer\mafba
4 of 14
No
No
Drer\mafbb
4 of 14
No
No
Drer\mafb
4 of 14
No
No
Drer\nrl
4 of 14
No
No
Drer\mafa
3 of 14
No
No
Drer\atf6b
1 of 14
No
No
Drer\bach1a
1 of 14
No
Yes
Drer\nfe2
1 of 14
No
No
Drer\nfe2l2a
1 of 14
No
No
Caenorhabditis elegans (Nematode, roundworm) (2)
Cele\maf-1
7 of 14
Yes
Yes
Cele\mrck-1
1 of 14
No
No
Anopheles gambiae (African malaria mosquito) (4)
Agam\AgaP_AGAP010405
12 of 12
Yes
Yes
Agam\AgaP_AGAP010030
4 of 12
No
No
Agam\AgaP_AGAP005300
1 of 12
No
No
Agam\AgaP_AGAP010623
1 of 12
No
No
Arabidopsis thaliana (thale-cress) (0)
Saccharomyces cerevisiae (Brewer's yeast) (0)
Schizosaccharomyces pombe (Fission yeast) (0)
Escherichia coli (enterobacterium) (0)
Other Organism Orthologs (via OrthoDB)
Data provided directly from OrthoDB:maf-S. Refer to their site for version information.
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v9.1)
Gene Symbol
Score
Source
Compara
Domainoid
eggNOG
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Drosophila melanogaster (Fruit fly) (3)
tj
6 of 13
cnc
1 of 13
kay
1 of 13
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 0 )
    Human Ortholog
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 2 )
    Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    MAFK; MAF bZIP transcription factor K
    13 of 14
    600197
         
        MAFF; MAF bZIP transcription factor F
        11 of 14
        604877
             
            MAFG; MAF bZIP transcription factor G
            11 of 14
            602020
                 
                MAFA; MAF bZIP transcription factor A
                4 of 14
                610303
                  MAFB; MAF bZIP transcription factor B
                  4 of 14
                  608968
                  MAF; MAF bZIP transcription factor
                  4 of 14
                  177075
                  NRL; neural retina leucine zipper
                  4 of 14
                  162080
                  Functional Complementation Data
                  Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
                  Interactions
                  Summary of Physical Interactions
                  Summary of Genetic Interactions
                  Interaction Browsers
                  View in FlyBase Interactions Browser   View in MIST tool (external link)
                  Please look at the allele data for full details of the genetic interactions
                  Starting gene(s)
                  Interaction type
                  Interacting gene(s)
                  Reference
                  Starting gene(s)
                  Interaction type
                  Interacting gene(s)
                  Reference
                  cnc
                  enhanceable
                  maf-S
                  (Rahman et al., 2013)
                  cnc
                  suppressible
                  maf-S
                  (Rahman et al., 2013)
                  dom
                  suppressible
                  maf-S
                  (Ellis et al., 2015)
                  External Data
                  Linkouts
                  BioGRID - A database of protein and genetic interactions.
                  DroID - A comprehensive database of gene and protein interactions.
                  MIST (genetic) - An integrated Molecular Interaction Database
                  MIST (protein-protein) - An integrated Molecular Interaction Database
                  Pathways
                  Signaling Pathways (FlyBase)
                  Metabolic Pathways
                  FlyBase
                  External Links
                  External Data
                  Linkouts
                  Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
                  Class of Gene
                  Genomic Location and Detailed Mapping Data
                  Chromosome (arm)
                  2R
                  Recombination map
                  2-91
                  Cytogenetic map
                  57A8-57A8
                  Sequence location
                  FlyBase Computed Cytological Location
                  Cytogenetic map
                  Evidence for location
                  57A8-57A8
                  Limits computationally determined from genome sequence between P{EP}l(2)05510EP2587&P{lacW}rigk07917 and P{lacW}l(2)k02206k02206
                  Experimentally Determined Cytological Location
                  Cytogenetic map
                  Notes
                  References
                  57A-57A
                  (determined by in situ hybridisation)
                  (Vani et al., 1997)
                  Experimentally Determined Recombination Data
                  Location

                  2-91

                  (FlyBase, 2016)

                  2-98.1

                  (Comeron et al., 2012)
                  Left of (cM)
                  Right of (cM)
                  Notes
                  Stocks and Reagents
                  Stocks (12)
                  Genomic Clones (19)
                   

                  Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

                  cDNA Clones (20)
                   

                  Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

                  cDNA clones, fully sequenced
                  BDGP DGC clones
                  Other clones
                  Drosophila Genomics Resource Center cDNA clones

                  For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

                  cDNA Clones, End Sequenced (ESTs)
                  BDGP DGC clones
                  Other clones
                  RNAi and Array Information
                  Linkouts
                  DRSC - Results frm RNAi screens
                  Antibody Information
                  Laboratory Generated Antibodies
                   
                  Commercially Available Antibodies
                   
                  Cell Line Information
                  Publicly Available Cell Lines
                   
                    Other Stable Cell Lines
                     
                      Other Comments

                      Isolated in a yeast two-hybrid screen for proteins interacting with cnc.

                      (Mohler et al., 1997)
                      Relationship to Other Genes
                      Source for database merge of

                      Source for merge of: CG9954 anon- EST:Posey173

                      (Bayraktaroglu, 2000.11.2)

                      Source for merge of: maf-S maf2

                      (Mohler, 2006.5.4)
                      Additional comments
                      Nomenclature History
                      Source for database identify of

                      Source for identity of: maf-S CG9954

                      (Veraksa et al., 2000)
                      Nomenclature comments
                      Etymology
                      Synonyms and Secondary IDs (13)
                      Datasets (0)
                      Study focus (0)
                      Experimental Role
                      Project
                      Project Type
                      Title
                      Study result (0)
                      Result
                      Result Type
                      Title
                      External Crossreferences and Linkouts ( 39 )
                      Sequence Crossreferences
                      NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
                      GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
                      GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
                      RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
                      UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.
                      UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
                      Other crossreferences
                      AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.
                      BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
                      DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
                      EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
                      FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
                      FlyMine - An integrated database for Drosophila genomics
                      InterPro - A database of protein families, domains and functional sites
                      KEGG Genes - Molecular building blocks of life in the genomic space.
                      MARRVEL_MODEL - MARRVEL (model organism gene)
                      Linkouts
                      BioGRID - A database of protein and genetic interactions.
                      Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
                      DroID - A comprehensive database of gene and protein interactions.
                      DRSC - Results frm RNAi screens
                      Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
                      FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
                      FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
                      Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
                      Flygut - An atlas of the Drosophila adult midgut
                      iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
                      MIST (genetic) - An integrated Molecular Interaction Database
                      MIST (protein-protein) - An integrated Molecular Interaction Database
                      Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
                      References (79)