FlyBase Gene Report: Dmel\Snap29

General Information

Symbol

Dmel\Snap29

Species

D. melanogaster

Name

Synaptosomal-associated protein 29kDa

Annotation Symbol

CG11173

Feature Type

protein_coding_gene

FlyBase ID

FBgn0034913

Gene Model Status

Current

Stock Availability

11 publicly available

Gene Summary

Synaptosomal-associated protein 29kDa (Snap29) encodes a member of the SNARE (soluble N-ethylmaleimide-sensitive factor-attachment protein (SNAP) receptor) family. It is involved in regulation of autophagy, endocytic recycling and cell division. [Date last reviewed: 2018-10-18] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
Interactive Fly

Also Known As

usnp, ubisnap, SNAP-29

Key Links

Genomic Location

Cytogenetic map

60A3-60A3

Sequence location

2R:23,843,483..23,844,763 [-]

Recombination map

2-105

RefSeq locus

NT_033778 REGION:23843483..23844763

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse GBrowse

Help me choose

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

Gene Ontology (GO) Annotations (14 terms)

Molecular Function (3 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

enables SNAP receptor activity

inferred from physical interaction with FLYBASE:Syx17; FB:FBgn0035540, FLYBASE:Vamp7; FB:FBgn0266186

(Takáts et al., 2013)

enables SNARE binding

inferred from physical interaction with FLYBASE:Syb; FB:FBgn0003660

(Morelli et al., 2014)

inferred from physical interaction with FLYBASE:Syx1A; FB:FBgn0013343

(Morelli et al., 2014)

inferred from physical interaction with FLYBASE:Syx4; FB:FBgn0024980

(Morelli et al., 2014)

inferred from physical interaction with FLYBASE:Sec22; FB:FBgn0260855

(Morelli et al., 2014)

inferred from physical interaction with FLYBASE:Vamp7; FB:FBgn0266186

(Morelli et al., 2014)

inferred from physical interaction with FLYBASE:Syx7; FB:FBgn0267849

(Morelli et al., 2014)

inferred from physical interaction with FLYBASE:Syx17; FB:FBgn0035540, FLYBASE:Vamp7; FB:FBgn0266186

(Takáts et al., 2013)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

enables SNAP receptor activity

inferred from biological aspect of ancestor with PANTHER:PTN000447753

(assigned by GO_Central )

(Gaudet et al., 2011)

inferred from sequence or structural similarity

(Littleton, 2000)

enables syntaxin binding

inferred from biological aspect of ancestor with PANTHER:PTN000447753

(assigned by GO_Central )

(Gaudet et al., 2011)

Biological Process (9 terms)

Terms Based on Experimental Evidence (4 terms)

CV Term

Evidence

References

involved_in autophagosome maturation

inferred from mutant phenotype

(Morelli et al., 2014, Takáts et al., 2013)

involved_in Golgi organization

inferred from mutant phenotype

(Morelli et al., 2014)

involved_in negative regulation of secretion by cell

inferred from mutant phenotype

(Morelli et al., 2014)

involved_in regulation of endocytosis

inferred from mutant phenotype

(Morelli et al., 2014)

Terms Based on Predictions or Assertions (5 terms)

CV Term

Evidence

References

involved_in exocytosis

inferred from biological aspect of ancestor with PANTHER:PTN000447753

(assigned by GO_Central )

(Gaudet et al., 2011)

involved_in synaptic vesicle fusion to presynaptic active zone membrane

inferred from biological aspect of ancestor with PANTHER:PTN000447754

(assigned by GO_Central )

(Gaudet et al., 2011)

involved_in synaptic vesicle priming

inferred from biological aspect of ancestor with PANTHER:PTN000447754

(assigned by GO_Central )

(Gaudet et al., 2011)

involved_in vesicle fusion

inferred from biological aspect of ancestor with PANTHER:PTN000447753

(assigned by GO_Central )

(Gaudet et al., 2011)

involved_in vesicle-mediated transport

inferred from sequence or structural similarity

(Littleton, 2000)

Cellular Component (2 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

part_of SNARE complex

inferred from direct assay

(Takáts et al., 2013)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

located_in plasma membrane

inferred from biological aspect of ancestor with PANTHER:PTN000447752

(assigned by GO_Central )

(Gaudet et al., 2011)

part_of SNARE complex

inferred from biological aspect of ancestor with PANTHER:PTN000447753

(assigned by GO_Central )

(Gaudet et al., 2011)

Gene Group (FlyBase)

Qbc-SNAREs

Protein Family (UniProt)

Protein Signatures (InterPro)

Target SNARE coiled-coil homology domain

Summaries

Gene Snapshot

Gene Group (FlyBase)

Qbc-SNAREs -: Q-SNAREs are members of the SNARE (soluble N-ethylmaleimide-sensitive factor-attachment protein (SNAP) receptor) family that mediate vesicle-fusion. SNAREs are cytoplasmic, membrane-anchored proteins that contain a 60 amino acid SNARE domain. Three Q-SNAREs domains from the target membrane and one R-SNARE domain from the vesicle interact to form a four four-helix bundle that pulls the two membranes in close proximity and promotes fusion. After fusion, the complexes are dissembled by NSF (N-ethylmaleimide-sensitive factor) and SNAPs (Soluble NSF Attachment Proteins). Q-SNAREs are named from their contribution of a conserved glutamine (Q) residue to the helix bundle. Q-SNARE domains are designated a, b or c, depending on their position within the helix bundle. Qbc-SNAREs (or SNAPs (synaptosomal-associated proteins) possess a Qb and Qc domain, thus contribute two helices to the bundle. (Adapted from PMID:15893389 and FBrf0216182).

Summary (Interactive Fly)

during mitosis - Snap29 mutant imaginal discs exhibit impairment of a late step of autophagy

Interactive Fly

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Snap29 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Comments on Gene Model

Gene model reviewed during 5.52

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Snap29-RA

FBtr0072236

NM_079107

1212

284

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

Snap29-PA

FBpp0072145

31.6

284

4.80

NP_523831

AAF47071

Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Target SNARE coiled-coil homology domain

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Q9W1I8

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Snap29 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Snap29_1

Linkouts

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 11 -- 12

garland cell primordium

(Fisher et al., 2012)

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 13 -- 16

embryonic/larval garland cell

(Fisher et al., 2012)

organism | ubiquitous

(Fisher et al., 2012)

RT-PCR

Stage

Tissue/Position (including subcellular localization)

Reference

larval stage

eye disc

(Morelli et al., 2014)

wing disc

(Morelli et al., 2014)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

western blot

Stage

Tissue/Position (including subcellular localization)

Reference

larval stage

wing disc

(Morelli et al., 2014)

eye disc

(Morelli et al., 2014)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

part_of SNARE complex

inferred from direct assay

(Takáts et al., 2013)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Snap29 in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Snap29

View exonic expression by tissue for Dmel\Snap29

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0034913

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0034913

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG11173-RA

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG11173

Flygut - An atlas of the Drosophila adult midgut

FBgn0034913

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\Snap29

Stages(s) 1-3
Stages(s) 11-12
Stages(s) 13-16

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 2 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 16 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Snap29

Transgenic constructs containing regulatory region of Snap29

Aberrations (Deficiencies and Duplications) ( 3 )

Inferred from experimentation ( 3 )

Gene not disrupted in

Df(2R)egl2

(Morelli et al., 2014)

Gene disrupted in

Df(2R)3-659

(Morelli et al., 2014)

Df(2R)106

(Morelli et al., 2014)

Inferred from location ( 0 )

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal | pupal stage, with Scer\GAL4^pnr-MD237

Snap29^GD7222

partially lethal - majority die | pupal stage, with Scer\GAL4^pnr-MD237

Snap29^GD7222

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Snap29^GD7222

viable, with Scer\GAL4^Mef2.PR

Snap29^GD7222

viable, with Scer\GAL4^tin.CΔ4

Snap29^GD7222

Other Phenotypes

Allele

abnormal neuroanatomy | adult stage, with Scer\GAL4^ple.PU

Snap29^TRiP.cUa

short lived, with Scer\GAL4^Mef2.PR

Snap29^GD7222

visible, with Scer\GAL4^en.PU

Snap29^JF01883

Phenotype manifest in

Allele

autophagosome | somatic clone

Snap29^B6-21

dopaminergic PPL1 neuron, with Scer\GAL4^ple.PU

Snap29^TRiP.cUa

embryonic/larval fat body | third instar larval stage, with Scer\GAL4^Cg.PA

Snap29^JF01883

eye-antennal disc | somatic clone | third instar larval stage

Snap29^B6-21

Golgi cisterna | somatic clone

Snap29^B6-21

wing, with Scer\GAL4^en.PU

Snap29^JF01883

Orthologs

Downloads

Download All DIOPT Orthologs

Download All OrthoDB Orthologs

Human Orthologs (via DIOPT v8.0)

Homo sapiens (Human) (4)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Hsap\SNAP29

14 of 15

Yes

Hsap\SNAP23

2 of 15

Hsap\SNAP25

1 of 15

Hsap\SNAP47

1 of 15

Yes

Model Organism Orthologs (via DIOPT v8.0)

Mus musculus (laboratory mouse) (4)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Mmus\Snap29

14 of 15

Yes

Mmus\Snap23

1 of 15

Mmus\Snap25

1 of 15

Mmus\Snap47

1 of 15

Yes

Rattus norvegicus (Norway rat) (3)

Rnor\Snap29

13 of 13

Yes

Rnor\Snap23

1 of 13

Rnor\Snap47

1 of 13

Yes

Xenopus tropicalis (Western clawed frog) (4)

Xtro\snap29

13 of 12

Yes

Xtro\snap23

2 of 12

Xtro\snap25

1 of 12

Xtro\snap47

1 of 12

Yes

Danio rerio (Zebrafish) (6)

Drer\snap29

12 of 15

Yes

2 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Caenorhabditis elegans (Nematode, roundworm) (3)

Cele\snap-29

12 of 15

Yes

Cele\aex-4

1 of 15

Cele\ric-4

1 of 15

Arabidopsis thaliana (thale-cress) (3)

Atha\SNAP29

1 of 9

Yes

Atha\SNAP30

1 of 9

Yes

Atha\SNAP33

1 of 9

Yes

Saccharomyces cerevisiae (Brewer's yeast) (2)

Scer\SEC9

5 of 15

Yes

Scer\SPO20

3 of 15

Yes

Schizosaccharomyces pombe (Fission yeast) (1)

Spom\sec9

3 of 12

Yes

Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG09190DKX )

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila suzukii

Spotted wing Drosophila

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA10816

Drosophila persimilis

Dper\GL11066

Drosophila willistoni

Dwil\GK15806

Drosophila virilis

Dvir\GJ21482

Drosophila mojavensis

Dmoj\GI18402

Drosophila grimshawi

Dgri\GH22912

Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091508WQ )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Musca domestica

House fly

Mdoa\17018416

Lucilia cuprina

Australian sheep blowfly

Lcup\FF38_09371

Mayetiola destructor

Hessian fly

Mdes\Mdes007493

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL009280

Anopheles darlingi

American malaria mosquito

Adar\ADAC004377

Anopheles gambiae

Malaria mosquito

Agam\AGAP011135

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ011681

Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W0F1C )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Bombyx mori

Silkmoth

Bmor\BGIBMGA012985

Danaus plexippus

Monarch butterfly

Dple\DPOGS206893-PA

Heliconius melpomene

Postman butterfly

Hmel\HMEL006864

Apis florea

Little honeybee

Aflo\3955243380

Apis mellifera

Western honey bee

Amel\usnp

Bombus impatiens

Common eastern bumble bee

Bimp\BIMP17083

Bombus terrestris

Buff-tailed bumblebee

Bter\15203509

Linepithema humile

Argentine ant

Lhum\LH19265

Megachile rotundata

Alfalfa leafcutting bee

Mrot\MROTU:001995

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG005483

Dendroctonus ponderosae

Mountain pine beetle

Dpod\YQE_08400

Tribolium castaneum

Red flour beetle

Tcas\TC033325

Pediculus humanus

Human body louse

Phum\PHUM607940

Rhodnius prolixus

Kissing bug

Rpro\RPRC011013

Rhodnius prolixus

Kissing bug

Rpro\RPRC014243

Cimex lectularius

Bed bug

Clec\CLEC001049

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI005481

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_11765

Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X0MTI )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strigamia maritima

European centipede

Smar\SMAR003540

Ixodes scapularis

Black-legged tick

Isca\ISCW001316

Stegodyphus mimosarum

African social velvet spider

Smim\KFM78995.1

Tetranychus urticae

Two-spotted spider mite

Turt\tetur31g01350

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_194582

Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G0GKH )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Snap29

Ciona intestinalis

Vase tunicate

Cint\ENSCING00000004884

Gallus gallus

Domestic chicken

Ggal\SNAP29

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v8.0)

Drosophila melanogaster (Fruit fly) (2)

Gene Symbol

Score

Source

Alignment

3 of 10

3 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

CEDNIK syndrome

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Allele

Disease

Evidence

References

Snap29^B6-21

model of CEDNIK syndrome

CEA

(Morelli et al., 2014)

Potential Models Based on Orthology ( 1 )

Human Ortholog

Disease

Evidence

References

SNAP29; synaptosome associated protein 29

model of CEDNIK syndrome

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

SNAP29; synaptosome associated protein 29

14 of 15

604202

CEREBRAL DYSGENESIS, NEUROPATHY, ICHTHYOSIS, AND PALMOPLANTAR KERATODERMA SYNDROME

CEDNIK syndrome

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

View in Interactions Browser

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Snap29 - AttD

experimental knowledge based

(Guruharsha et al., 2011)

Snap29 - Bet1

experimental knowledge based

(Guruharsha et al., 2011)

Snap29 - Cenp-C

anti tag coimmunoprecipitation, peptide massfingerprinting

(Przewloka et al., 2011)

Snap29 - Dap160

pull down, western blot

(Xu et al., 2014)

Snap29 - Gos28

experimental knowledge based

(Guruharsha et al., 2011)

Snap29 - Membrin

experimental knowledge based

(Guruharsha et al., 2011)

Snap29 - Ndc80

anti tag coimmunoprecipitation, anti tag western blot

(Morelli et al., 2016)

Snap29 - Nsf2

anti bait coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based

(Morelli et al., 2014, Guruharsha et al., 2011)

Snap29 - Past1

experimental knowledge based, pull down, western blot

(Xu et al., 2014, Guruharsha et al., 2011)

Snap29 - Rme-8

experimental knowledge based

(Guruharsha et al., 2011)

Snap29 - Sec20

experimental knowledge based

(Guruharsha et al., 2011)

Snap29 - Sec22

experimental knowledge based, anti bait coimmunoprecipitation, peptide massfingerprinting

(Morelli et al., 2014, Guruharsha et al., 2011)

Snap29 - Slh

experimental knowledge based

(Guruharsha et al., 2011)

Snap29 - Snap24

experimental knowledge based

(Guruharsha et al., 2011)

Snap29 - Snap25

experimental knowledge based

(Guruharsha et al., 2011)

Snap29 - Syb

anti bait coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based

(Morelli et al., 2014, Guruharsha et al., 2011)

Snap29 - Syx13

anti tag coimmunoprecipitation, western blot, experimental knowledge based, anti tag western blot

(Csizmadia et al., 2018, Guruharsha et al., 2011)

Snap29 - Syx16

pull down, western blot, experimental knowledge based, anti bait coimmunoprecipitation

(Xu et al., 2014, Guruharsha et al., 2011)

Snap29 - Syx17

anti bait coimmunoprecipitation, western blot, anti tag coimmunoprecipitation, anti tag western blot, pull down, molecular weight estimation by staining, experimental knowledge based

(Takáts et al., 2018, Takáts et al., 2014, Takáts et al., 2013, Guruharsha et al., 2011)

Snap29 - Syx18

experimental knowledge based

(Guruharsha et al., 2011)

Snap29 - Syx1A

anti bait coimmunoprecipitation, western blot, pull down, experimental knowledge based, peptide massfingerprinting

(Morelli et al., 2014, Xu et al., 2014, Guruharsha et al., 2011)

Snap29 - Syx4

experimental knowledge based, anti bait coimmunoprecipitation, peptide massfingerprinting

(Morelli et al., 2014, Guruharsha et al., 2011)

Snap29 - Syx5

experimental knowledge based

(Guruharsha et al., 2011)

Snap29 - Syx7

experimental knowledge based, anti bait coimmunoprecipitation, peptide massfingerprinting

(Morelli et al., 2014, Guruharsha et al., 2011)

Snap29 - Syx8

experimental knowledge based

(Guruharsha et al., 2011)

Snap29 - Use1

experimental knowledge based

(Guruharsha et al., 2011)

Snap29 - Vamp7

anti bait coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based

(Morelli et al., 2014, Guruharsha et al., 2011)

Snap29 - Vti1a

experimental knowledge based

(Guruharsha et al., 2011)

Snap29 - Ykt6

experimental knowledge based

(Guruharsha et al., 2011)

Snap29 - koko

experimental knowledge based

(Guruharsha et al., 2011)

Snap29 - nSyb

experimental knowledge based

(Guruharsha et al., 2011)

Snap29 - αSnap

pull down, western blot, anti bait coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based

(Morelli et al., 2014, Xu et al., 2014, Guruharsha et al., 2011)

Snap29 - γSnap1

anti bait coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based

(Morelli et al., 2014, Guruharsha et al., 2011)

Snap29 - γSnap2

experimental knowledge based

(Guruharsha et al., 2011)

Summary of Genetic Interactions

esyN Network Diagram

esyN Network Key:

Suppression

Enhancement

View in Interactions Browser

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Snap29

enhanceable

Sec22

(Morelli et al., 2014)

Snap29

suppressible

Socs36E

(Morelli et al., 2014)

Snap29

enhanceable

Syx17

(Morelli et al., 2014)

Snap29

enhanceable

Vamp7

(Morelli et al., 2014)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

63369

DroID - A comprehensive database of gene and protein interactions.

FBgn0034913

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

37774

MIST (protein-protein) - An integrated Molecular Interaction Database

Snap29

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

External Data

Linkouts

KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-105

Cytogenetic map

60A3-60A3

Sequence location

2R:23,843,483..23,844,763 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

60A3-60A3

Limits computationally determined from genome sequence between P{EP}egl^EP938 and P{lacW}eIF6^k13214

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

60A3-60A4

(Boulianne, 2000.6.6)

Experimentally Determined Recombination Data

Location

2-105

(FlyBase, 2016)

2-109.6

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (11)

Bloomington

56817

w^*; M{UAS-CFP.Snap29}ZH-86Fb

81905

w^*; PBac{U6:3-Snap29.gRNA}VK00027

25862

y¹ v¹; P{TRiP.JF01883}attP2

51893

y¹ v¹; P{TRiP.HMC03467}attP40

81907

w^*; PBac{U6:3-Snap24-Snap25-Snap29.gRNA}VK00027

78220

y¹ sc^* v¹ sev²¹; P{TOE.GS01406}attP40

83927

y¹ sc^* v¹ sev²¹; P{TKO.GS03522}attP40/CyO

56818

w^*; P{neoFRT}42D Snap29^B6-21/CyO, P{GAL4-twi.G}2.2, P{UAS-2xEGFP}AH2.2

VDRC

v107947

P{KK108034}VIE-260B

v18172

w¹¹¹⁸; P{GD7222}v18172

v318224

PBac{fTRG00738.sfGFP-TVPTBF}VK00033

Genomic Clones (19)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (19)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

LD03592

Other clones

DPiM_LD03592

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0034913

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0034913

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

37774

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Gordon et al., 2017, Morelli et al., 2014, Xu et al., 2014, Takáts et al., 2013)

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for identity of: usnp CG11173

(Boulianne, 2000.6.6)

Source for identity of: Snap29 usnp

(FlyBase, 2009-)

Source for database merge of

Additional comments

Renamed from 'usnp' to 'Snap29' to better reflect usage in the literature and to improve consistency in naming of SNAP genes.

(FlyBase, 2009-)

Other Comments

Snap29 is required for the fusion of autophagosomes with degradative endocytic organelles. It is also required for proper Golgi apparatus morphology and has an inhibitory role in membrane fusion at the apical plasma membrane.

(Morelli et al., 2014)

Identification: as a protein that interacts strongly with Snap in a yeast two-hybrid system.

(Xu et al., 1999)

Origin and Etymology

Discoverer

Etymology

Identification

External Crossreferences and Linkouts ( 36 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

37774

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Q9W1I8

Other crossreferences

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0034913

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0034913

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Snap29_1

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG11173

Flygut - An atlas of the Drosophila adult midgut

FBgn0034913

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

37774

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC033325

InterPro - A database of protein families, domains and functional sites

Target SNARE coiled-coil homology domain

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG11173

modMine - A data warehouse for the modENCODE project

FBgn0034913

Linkouts

BioGRID - A database of protein and genetic interactions.

63369

DPiM - Drosophila Protein interaction map

FBgn0034913

DroID - A comprehensive database of gene and protein interactions.

FBgn0034913

DRSC - Results frm RNAi screens

FBgn0034913

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0034913

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG11173-RA

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0034913

FlyMine - An integrated database for Drosophila genomics

FBgn0034913

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

37774

KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.

MIST (protein-protein) - An integrated Molecular Interaction Database

Snap29

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Synonyms and Secondary IDs (13)

Reported As

Symbol Synonym

CG11173

(Hosono et al., 2015, Ni et al., 2010.12.1, Keleman et al., 2009.8.5, Perkins et al., 2009.1.15, Dorer et al., 2006, Bock, 2000.10.26, Boulianne, 2000.6.6, Niemeyer and Schwarz, 2000)

MENE (2R)-E

(Vaccari et al., 2009)

MENE(2R)-E

(Vaccari et al., 2009)

SNAP-29

(Zhang and Baehrecke, 2015, Dorer et al., 2006, Kirby et al., 2004, Littleton, 2000, Niemeyer and Schwarz, 2000)

SNAP29

(Fujita et al., 2017, Kloepper et al., 2008)

Snap29

(Cunningham et al., 2020, González-Méndez et al., 2020, Maurer et al., 2020, Xu et al., 2020, Kriegenburg et al., 2019, Meltzer et al., 2019, Poe et al., 2019, Texada et al., 2019, Csizmadia et al., 2018, Manent et al., 2017, Transgenic RNAi Project members, 2017-, Morelli et al., 2016, Morelli et al., 2014, Vaccari, 2014.8.4)

Ubisnap

(Xu et al., 1999)

Usnp

(Warecki et al., 2020, Stephano et al., 2018)

dSNAP-29

(Xu et al., 2014)

snap29

(González-Méndez et al., 2020, Robinson et al., 2020)

ubisnap

(Babcock et al., 2015, Mulakkal et al., 2014, Takáts et al., 2014)

usnp

(Lőrincz et al., 2016, Sarov et al., 2016, Takáts et al., 2014, Kwon et al., 2013, Oortveld et al., 2013, Takáts et al., 2013, Younger et al., 2013, Japanese National Institute of Genetics, 2012.5.21, Hendrix et al., 2008, Stuart et al., 2007)

Name Synonyms

Synaptosomal-associated protein 29kDa

Ubisnap

(Przewloka et al., 2011)

ubisnap

(Lőrincz et al., 2016, Hegedűs et al., 2013, Xu et al., 1999)

Secondary FlyBase IDs

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

References (78)

The following external sites may use different assemblies or annotations than FlyBase.

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