FB2021_02, released April 13, 2021

Gene: Dmel\Eps-15

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General Information
Symbol
Dmel\Eps-15
Species
D. melanogaster
Name
Epidermal growth factor receptor pathway substrate clone 15
Annotation Symbol
CG16932
Feature Type
protein_coding_gene
FlyBase ID
FBgn0035060
Gene Model Status
Current
Stock Availability
13 publicly available
Gene Summary
Epidermal growth factor receptor pathway substrate clone 15 (Eps-15) encodes a multi-domain adaptor protein involved in endocytosis. It interacts with several other endocytic factors such as those encoded by shi, stnB, Dap160 and AP-2α. It is enriched at sites of endocytosis and is required for normal synaptic bouton development and vesicle endocytosis during high frequency stimulation. [Date last reviewed: 2018-10-18] (FlyBase Gene Snapshot)
All Summaries
Also Known As

Eps15

Key Links
Genomic Location
Cytogenetic map
60E1-60E1
Sequence location
2R:24,768,314..24,775,265 [-]
Recombination map
2-107
RefSeq locus
NT_033778 REGION:24768314..24775265
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
GO Summary Ribbons
Gene Ontology (GO) Annotations (9 terms)
Molecular Function (1 term)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
enables calcium ion binding
inferred from electronic annotation with InterPro:IPR002048
(assigned by InterPro )
(FlyBase Curators et al., 2004-)
Biological Process (5 terms)
Terms Based on Experimental Evidence (3 terms)
CV Term
Evidence
References
involved_in negative regulation of Notch signaling pathway
inferred from mutant phenotype
(Tang et al., 2005)
involved_in regulation of synaptic growth at neuromuscular junction
inferred from mutant phenotype
(Koh et al., 2007)
involved_in synaptic vesicle endocytosis
inferred from mutant phenotype
(Koh et al., 2007)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
involved_in endocytosis
inferred from biological aspect of ancestor with PANTHER:PTN000130974
(assigned by GO_Central )
(Gaudet et al., 2011)
involved_in endosomal transport
inferred from biological aspect of ancestor with PANTHER:PTN000130974
(assigned by GO_Central )
(Gaudet et al., 2011)
Cellular Component (3 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
located_in cytoplasm
inferred from high throughput direct assay
(Lee et al., 2014)
colocalizes_with synaptic vesicle
inferred from direct assay
(Koh et al., 2007)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
part_of clathrin coat of coated pit
inferred from biological aspect of ancestor with PANTHER:PTN001677755
(assigned by GO_Central )
(Gaudet et al., 2011)
Gene Group (FlyBase)
Pathway (FlyBase)
Protein Family (UniProt)
-
Protein Signatures (InterPro)
Summaries
Gene Snapshot
Epidermal growth factor receptor pathway substrate clone 15 (Eps-15) encodes a multi-domain adaptor protein involved in endocytosis. It interacts with several other endocytic factors such as those encoded by shi, stnB, Dap160 and AP-2α. It is enriched at sites of endocytosis and is required for normal synaptic bouton development and vesicle endocytosis during high frequency stimulation. [Date last reviewed: 2018-10-18]
Pathway (FlyBase)
Negative Regulators of Notch Signaling Pathway -
The Notch receptor signaling pathway is activated by the binding of the transmembrane receptor Notch (N) to transmembrane ligands, Dl or Ser, presented on adjacent cells. This results in the proteolytic cleavage of N, releasing the intracellular domain (NICD). NICD translocates into the nucleus, interacting with Su(H) and mam to form a transcription complex, which up-regulates transcription of Notch-responsive genes. Negative regulators of the pathway down-regulate the signal from the sending cell or the response in the receiving cell. (Adapted from FBrf0225731 and FBrf0192604).
Gene Model and Products
Number of Transcripts
4
Number of Unique Polypeptides
4

Please see the JBrowse view of Dmel\Eps-15 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model

Gene model reviewed during 5.39

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.52

Sequence Ontology: Class of Gene
nuclear_gene
protein_coding_gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
Eps-15-RA
FBtr0072435
NM_138121
4421
1253
Eps-15-RB
FBtr0072434
NM_166687
4358
1232
Eps-15-RC
FBtr0072433
NM_166686
3980
1106
Eps-15-RD
FBtr0308684
NM_001259573
3695
792
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
Eps-15-PA
FBpp0072340
134.4
1253
4.82
NP_611965
AAF47267
Eps-15-PB
FBpp0072339
132.1
1232
4.81
NP_726482
AAM70793
Eps-15-PC
FBpp0072338
119.6
1106
4.74
NP_726481
AAM70792
Eps-15-PD
FBpp0300890
85.5
792
4.91
NP_001246502
AFH08255
Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Eps-15 using the Feature Mapper tool.

External Data
Crossreferences
Linkouts
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 10 -- 15
presumptive embryonic/larval central nervous system
(Tang et al., 2005)
embryonic stage 16
embryonic/larval central nervous system
(Tang et al., 2005)
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage
photoreceptor cell R1 | restricted
(Hamanaka and Meinertzhagen, 2010)
photoreceptor cell R2 | restricted
(Hamanaka and Meinertzhagen, 2010)
photoreceptor cell R3 | restricted
(Hamanaka and Meinertzhagen, 2010)
photoreceptor cell R4 | restricted
(Hamanaka and Meinertzhagen, 2010)
photoreceptor cell R5 | restricted
(Hamanaka and Meinertzhagen, 2010)
photoreceptor cell R6 | restricted
(Hamanaka and Meinertzhagen, 2010)
medulla | restricted
(Hamanaka and Meinertzhagen, 2010)
lamina | restricted
(Hamanaka and Meinertzhagen, 2010)
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage
adult head
(Aradska et al., 2015)
Additional Descriptive Data

Eps-15 is expressed in central brain neuropils. It is uniformly high amongst the different neuropils. In cross sections of the lamina, Eps-15 labelling reveals the outline of photoreceptor R1-R6 terminals, and particularly strong staining was seen at the sites facing the interior of the cartridge. Via immunoelectron microscopy, Eps-15 is seen amongst the vesicle pool in the cytoplasm of the photoreceptors, and often close to the capitate projection (invaginations from surrounding epithelial glial and specialised organelles for the endocytosis of vesicle membrane).

(Hamanaka and Meinertzhagen, 2010)
Marker for
 
Subcellular Localization
CV Term
Evidence
References
located_in cytoplasm
inferred from high throughput direct assay
(Lee et al., 2014)
colocalizes_with synaptic vesicle
inferred from direct assay
(Koh et al., 2007)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Eps-15 in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
FLIGHT - Cell culture data for RNAi and other high-throughput technologies
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 10 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 15 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of Eps-15
Transgenic constructs containing regulatory region of Eps-15
Aberrations (Deficiencies and Duplications) ( 6 )
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
lethal
lethal (with Df(2R)BSC606)
lethal | recessive, with Scer\GAL4- :
lethal - all die before end of larval stage (with Df(2R)Dll-MP)
lethal - all die before end of P-stage
lethal - all die before end of P-stage (with Eps-15e75)
lethal - all die before end of P-stage (with Eps-15Δ29)
lethal - all die before end of pupal stage
lethal - all die before end of pupal stage (with Df(2R)Dll-MP)
lethal - all die before end of pupal stage (with Eps-152)
lethal - all die before end of pupal stage (with Eps-15EP2513)
partially lethal - majority die
viable, with Dcr-2UAS.cDa, Scer\GAL4elav.PLu
viable, with Scer\GAL4Mef2.PR
viable, with Scer\GAL4pnr-MD237
viable, with Scer\GAL4tin.CΔ4
Sterility
Allele
fertile
Other Phenotypes
Allele
abnormal neuroanatomy, with Scer\GAL4da.G32
abnormal neuroanatomy | larval stage (with Df(2R)Dll-MP)
abnormal neuroanatomy | larval stage (with Eps-15e75)
abnormal neuroanatomy | larval stage (with Eps-15Δ29)
abnormal neurophysiology, with Scer\GAL4- :
abnormal neurophysiology | larval stage (with Df(2R)Dll-MP)
abnormal neurophysiology | larval stage (with Eps-15e75)
abnormal neurophysiology | larval stage (with Eps-15Δ29)
abnormal pain response | adult stage, with Dcr-2UAS.cDa, Scer\GAL4elav.PLu
some die during pharate adult stage
some die during pharate adult stage (with Eps-15e75)
some die during pharate adult stage (with Eps-15Δ29)
some die during pupal stage
some die during pupal stage (with Df(2R)Dll-MP)
some die during pupal stage (with Eps-152)
some die during pupal stage (with Eps-15EP2513)
some die during third instar larval stage (with Df(2R)Dll-MP)
visible, with Scer\GAL4en.PU
Phenotype manifest in
Allele
bouton (with Df(2R)Dll-MP)
bouton (with Eps-15e75)
bouton (with Eps-15Δ29)
bouton | increased number (with Df(2R)Dll-MP)
bouton | increased number (with Eps-15e75)
bouton | increased number (with Eps-15Δ29)
embryonic/larval neuromuscular junction
embryonic/larval neuromuscular junction (with Df(2R)Dll-MP)
embryonic/larval neuromuscular junction (with Eps-15e75)
embryonic/larval neuromuscular junction (with Eps-15Δ29)
eo sensory structure | increased number, with Scer\GAL4sca-537.4
eye disc, with Scer\GAL4da.G32
glial cell, with Scer\GAL4da.G32
macrochaeta, with Scer\GAL4sca-537.4
macrochaeta (with Df(2R)Dll-MP), with Scer\GAL4sca-537.4
macrochaeta (with Eps-152), with Scer\GAL4sca-537.4
macrochaeta (with Eps-15EP2513), with Scer\GAL4sca-537.4
macrochaeta | increased number, with Scer\GAL4sca-537.4
tormogen cell, with Scer\GAL4sca-537.4
tormogen cell (with Df(2R)Dll-MP), with Scer\GAL4sca-537.4
tormogen cell (with Eps-152), with Scer\GAL4sca-537.4
tormogen cell (with Eps-15EP2513), with Scer\GAL4sca-537.4
tormogen cell | increased number, with Scer\GAL4sca-537.4
wing, with Scer\GAL4Ilp2.PR
Orthologs
Downloads
Download All DIOPT Orthologs
Download All OrthoDB Orthologs
Human Orthologs (via DIOPT v8.0)
Homo sapiens (Human) (8)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Hsap\EPS15L1
13 of 15
Yes
Yes
1  
Hsap\EPS15
11 of 15
No
Yes
Hsap\EHD2
1 of 15
No
No
2  
Hsap\EHD3
1 of 15
No
No
1  
Hsap\ITSN1
1 of 15
No
No
Hsap\ITSN2
1 of 15
No
No
1  
Hsap\REPS1
1 of 15
No
No
Hsap\SYNRG
1 of 15
No
Yes
Model Organism Orthologs (via DIOPT v8.0)
Mus musculus (laboratory mouse) (8)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Mmus\Eps15l1
13 of 15
Yes
Yes
Mmus\Eps15
10 of 15
No
Yes
Mmus\Ehd2
1 of 15
No
No
Mmus\Ehd3
1 of 15
No
No
Mmus\Itsn1
1 of 15
No
No
Mmus\Itsn2
1 of 15
No
No
Mmus\Reps1
1 of 15
No
No
Mmus\Synrg
1 of 15
No
Yes
Rattus norvegicus (Norway rat) (7)
Rnor\Eps15l1
11 of 13
Yes
Yes
Rnor\Eps15
5 of 13
No
Yes
Rnor\Ehd2
1 of 13
No
No
Rnor\Ehd3
1 of 13
No
No
Rnor\Itsn1
1 of 13
No
No
Rnor\Itsn2
1 of 13
No
No
Rnor\LOC103689946
1 of 13
No
Yes
Xenopus tropicalis (Western clawed frog) (7)
Xtro\eps15l1
8 of 12
Yes
Yes
Xtro\eps15
6 of 12
No
Yes
Xtro\ehd2
1 of 12
No
No
Xtro\ehd3
1 of 12
No
No
Xtro\itsn1
1 of 12
No
No
Xtro\itsn2
1 of 12
No
No
Xtro\synrg
1 of 12
No
Yes
Danio rerio (Zebrafish) (12)
Drer\eps15l1a
12 of 15
Yes
Yes
Drer\eps15
10 of 15
No
Yes
Drer\eps15l1b
5 of 15
No
Yes
Drer\si:ch73-140j24.4
2 of 15
No
Yes
Drer\ehd2a
1 of 15
No
No
Drer\ehd2b
1 of 15
No
No
Drer\ehd3
1 of 15
No
No
Drer\itsn1
1 of 15
No
No
Drer\itsn2a
1 of 15
No
No
Drer\itsn2b
1 of 15
No
No
Drer\reps1
1 of 15
No
No
Drer\synrg
1 of 15
No
Yes
Caenorhabditis elegans (Nematode, roundworm) (3)
Cele\ehs-1
13 of 15
Yes
Yes
Cele\R10E11.6
1 of 15
No
Yes
Cele\reps-1
1 of 15
No
No
Arabidopsis thaliana (thale-cress) (2)
Atha\AT1G20760
4 of 9
Yes
Yes
Atha\AT1G21630
4 of 9
Yes
Yes
Saccharomyces cerevisiae (Brewer's yeast) (6)
Scer\EDE1
10 of 15
Yes
Yes
Scer\END3
3 of 15
No
Yes
Scer\IRS4
3 of 15
No
Yes
Scer\TAX4
3 of 15
No
Yes
Scer\PAN1
2 of 15
No
No
Scer\TPM1
1 of 15
No
No
Schizosaccharomyces pombe (Fission yeast) (5)
Spom\SPBC800.10c
8 of 12
Yes
Yes
Spom\ucp8
5 of 12
No
Yes
Spom\end3
2 of 12
No
Yes
Spom\SPAC1687.09
2 of 12
No
Yes
Spom\pan1
1 of 12
No
No
Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG091901LX )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila simulans
Dsim\GD24921
Drosophila sechellia
Dsec\GM11795
Drosophila erecta
Dere\GG19894
Drosophila yakuba
Dyak\GE11419
Drosophila ananassae
Dana\GF14485
Drosophila pseudoobscura pseudoobscura
Dpse\GA14224
Drosophila persimilis
Dper\GL17701
Drosophila willistoni
Dwil\GK21653
Drosophila virilis
Dvir\GJ21083
Drosophila mojavensis
Dmoj\GI19514
Drosophila grimshawi
Dgri\GH21541
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091503PQ )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Mdoa\17017453
Glossina morsitans
Tsetse fly
Gmor\GMOY010888
Lucilia cuprina
Australian sheep blowfly
Lcup\FF38_11522
Mayetiola destructor
Hessian fly
Mdes\Mdes008683
Mayetiola destructor
Hessian fly
Mdes\Mdes015494
Aedes aegypti
Yellow fever mosquito
Aaeg\AAEL007950
Anopheles darlingi
American malaria mosquito
Adar\ADAC008164
Anopheles gambiae
Malaria mosquito
Agam\GSTe8
Culex quinquefasciatus
Southern house mosquito
Cqui\CPIJ018635
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W016Q )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Bmor\BGIBMGA012628
Danaus plexippus
Monarch butterfly
Dple\DPOGS210526-PA
Apis florea
Little honeybee
Aflo\2568059620
Apis mellifera
Western honey bee
Amel\GB44307
Bombus impatiens
Common eastern bumble bee
Bimp\BIMP19568
Bombus terrestris
Buff-tailed bumblebee
Bter\15209950
Linepithema humile
Argentine ant
Lhum\LH13250
Linepithema humile
Argentine ant
Lhum\LH13248
Megachile rotundata
Alfalfa leafcutting bee
Mrot\MROTU:000d7f
Nasonia vitripennis
Parasitic wasp
Nvit\Nasvi2EG019743
Nasonia vitripennis
Parasitic wasp
Nvit\Nasvi2EG036388
Dendroctonus ponderosae
Mountain pine beetle
Dpod\YQE_11273
Tribolium castaneum
Red flour beetle
Tcas\TC015931
Pediculus humanus
Human body louse
Phum\PHUM591970
Rhodnius prolixus
Kissing bug
Rpro\RPRC007263
Cimex lectularius
Bed bug
Clec\CLEC003446
Acyrthosiphon pisum
Pea aphid
Apim\ACYPI004565
Acyrthosiphon pisum
Pea aphid
Apim\ACYPI084991
Zootermopsis nevadensis
Nevada dampwood termite
Znev\Znev_06003
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X0151 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strigamia maritima
European centipede
Smar\SMAR000118
Ixodes scapularis
Black-legged tick
Isca\ISCW001324
Stegodyphus mimosarum
African social velvet spider
Smim\KFM78649.1
Stegodyphus mimosarum
African social velvet spider
Smim\KFM78650.1
Tetranychus urticae
Two-spotted spider mite
Turt\tetur11g02520
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G01RG )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strongylocentrotus purpuratus
Purple sea urchin
Spur\SPU_008123gn
Strongylocentrotus purpuratus
Purple sea urchin
Spur\SPU_024295gn
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Eps15l1l
Ciona intestinalis
Vase tunicate
Cint\ENSCING00000009283
Gallus gallus
Domestic chicken
Ggal\EPS15L1
Gallus gallus
Domestic chicken
Ggal\EPS15
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v8.0)
Drosophila melanogaster (Fruit fly) (4)
Gene Symbol
Score
Source
Compara
eggNOG
Homologene
Inparanoid
Isobase
OrthoDB
OrthoFinder
orthoMCL
Panther
RoundUp
Alignment
CG9297
2 of 10
Dap160
2 of 10
Past1
2 of 10
Reps
1 of 10
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Model Summary Ribbon
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 0 )
    Human Ortholog
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 2 )
    Allele
    Disease
    Interaction
    References
    Eps-15e75
    exacerbates  Troyer syndrome
    modeled by spartin1
    (Nahm et al., 2013)
    Eps-15EP2513
    ameliorates  severe acute respiratory syndrome
    modeled by HCoV-SARS\3aUAS.CUHK-W1.EGFP
    (Wong et al., 2005)
    Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    EPS15L1; epidermal growth factor receptor pathway substrate 15 like 1
    13 of 15
    616826
         
        EPS15; epidermal growth factor receptor pathway substrate 15
        11 of 15
        600051
            Functional Complementation Data
            Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
            Interactions
            Summary of Physical Interactions
            Summary of Genetic Interactions
            esyN Network Diagram
            esyN Network Key:
            Suppression
            Enhancement
            View in Interactions Browser
            Please look at the allele data for full details of the genetic interactions
            Starting gene(s)
            Interaction type
            Interacting gene(s)
            Reference
            Eps-15
            suppressible
            Su(H)
            (Tang et al., 2005)
            Starting gene(s)
            Interaction type
            Interacting gene(s)
            Reference
            H
            enhanceable
            Eps-15
            (Tang et al., 2005)
            rho
            suppressible
            Eps-15
            (Tjota et al., 2011)
            External Data
            Linkouts
            BioGRID - A database of protein and genetic interactions.
            DroID - A comprehensive database of gene and protein interactions.
            InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
            MIST (protein-protein) - An integrated Molecular Interaction Database
            Pathways
            Signaling Pathways (FlyBase)
            Negative Regulators of Notch Signaling Pathway -
            The Notch receptor signaling pathway is activated by the binding of the transmembrane receptor Notch (N) to transmembrane ligands, Dl or Ser, presented on adjacent cells. This results in the proteolytic cleavage of N, releasing the intracellular domain (NICD). NICD translocates into the nucleus, interacting with Su(H) and mam to form a transcription complex, which up-regulates transcription of Notch-responsive genes. Negative regulators of the pathway down-regulate the signal from the sending cell or the response in the receiving cell. (Adapted from FBrf0225731 and FBrf0192604).
            Metabolic Pathways
            External Data
            Linkouts
            KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.
            Genomic Location and Detailed Mapping Data
            Chromosome (arm)
            2R
            Recombination map
            2-107
            Cytogenetic map
            60E1-60E1
            Sequence location
            2R:24,768,314..24,775,265 [-]
            FlyBase Computed Cytological Location
            Cytogenetic map
            Evidence for location
            60E1-60E1
            Limits computationally determined from genome sequence between P{lacW}Mmp1k04809 and P{EP}Reg-5EP1065
            Experimentally Determined Cytological Location
            Cytogenetic map
            Notes
            References
            60E-60E
            (Lloyd et al., 2000)
            Determined by comparing Celera genomic sequence with sequence from BDGP BAC and P1 clones.
            (Lloyd et al., 2000)
            Experimentally Determined Recombination Data
            Location

            2-107

            (FlyBase, 2016)

            2-111.4

            (Comeron et al., 2012)
            Left of (cM)
            Right of (cM)
            Notes
            Stocks and Reagents
            Stocks (13)
            Genomic Clones (24)
            cDNA Clones (100)
             

            Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

            cDNA clones, fully sequenced
            BDGP DGC clones
            Other clones
            Drosophila Genomics Resource Center cDNA clones

            For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

            cDNA Clones, End Sequenced (ESTs)
            BDGP DGC clones
            Other clones
            RNAi and Array Information
            Linkouts
            DRSC - Results frm RNAi screens
            GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
            Antibody Information
            Laboratory Generated Antibodies
             

            monoclonal

            (Halder et al., 2011)

            polyclonal

            (Koh et al., 2007, Korolchuk et al., 2007)
            Commercially Available Antibodies
             
            Other Information
            Relationship to Other Genes
            Source for database identify of

            Source for identity of: Eps-15 CG16932

            (Verstreken and Bellen, 2000.9.4)
            Source for database merge of

            Source for merge of: Eps-15 anon-WO0118547.85

            (FlyBase, 1996-)
            Additional comments

            Source for merge of Eps-15 anon-WO0118547.85 was sequence comparison ( date:051113 ).

            (FlyBase, 1996-)
            Other Comments

            dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.

            (Kiger et al., 2003)

            Area matching Drosophila EST AI260872 (inverted). This EST has sequence similarity to mouse signalling factor U29156.

            (Davies, 2001.3.15)
            Origin and Etymology
            Discoverer
            Etymology
            Identification
            External Crossreferences and Linkouts ( 51 )
            Sequence Crossreferences
            NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
            GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
            GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
            RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
            UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
            Other crossreferences
            Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
            Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
            Flygut - An atlas of the Drosophila adult midgut
            GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
            iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
            InterPro - A database of protein families, domains and functional sites
            KEGG Genes - Molecular building blocks of life in the genomic space.
            modMine - A data warehouse for the modENCODE project
            Linkouts
            BioGRID - A database of protein and genetic interactions.
            DroID - A comprehensive database of gene and protein interactions.
            DRSC - Results frm RNAi screens
            FLIGHT - Cell culture data for RNAi and other high-throughput technologies
            FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
            FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
            FlyMine - An integrated database for Drosophila genomics
            InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
            KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.
            MIST (protein-protein) - An integrated Molecular Interaction Database
            Synonyms and Secondary IDs (13)
            Reported As
            Symbol Synonym
            Aa2/2
            (Tsai et al., 2019)
            CG16932
            (Jensen et al., 2013, Ni et al., 2010.12.1, Keleman et al., 2009.8.5, Perkins et al., 2009, Majumdar et al., 2006, Metta et al., 2006, Molnar et al., 2006, Tang et al., 2005, Battersby et al., 2003)
            EH1
            (Tang et al., 2005)
            EPS-15
            (Tsai et al., 2019, Hamanaka and Meinertzhagen, 2010, Lloyd et al., 2000)
            EPS15
            (Wu et al., 2014, Tjota et al., 2011)
            Eps-15
            (Hope et al., 2020, Hall et al., 2019, Gene Disruption Project members, 2018-, Lee et al., 2018, Chen et al., 2015, Li et al., 2015, Ramirez et al., 2015, Ashwal-Fluss et al., 2014, Lee et al., 2014, Mukherjee et al., 2014, Kwon et al., 2013, Kwon et al., 2013, Friedman et al., 2011, Christensen et al., 2009.7.22, Venken et al., 2009, Christensen et al., 2008.9.3, Christensen et al., 2008.9.3, Christensen et al., 2008.9.3, Christensen et al., 2008.9.3, Koh, 2008.12.18, Stanyon et al., 2004)
            Eps15
            (Li et al., 2020, Yu et al., 2020, Latcheva et al., 2019, Gan and Watanabe, 2018, Li et al., 2015, Winther et al., 2015, Jensen et al., 2013, Xie et al., 2012, Franco et al., 2011, Wang and Pai, 2011, Yuva-Aydemir et al., 2011, Li et al., 2010, Giagtzoglou et al., 2009, Peng et al., 2009, Koh et al., 2007, Korolchuk et al., 2007, Majumdar et al., 2006, Molnar et al., 2006, Venken et al., 2006, Verstreken and Bellen, 2006, Crosetto et al., 2005, Tang et al., 2005, Wong et al., 2005, Gonzalez-Gaitan, 2003, Wairkar et al., 2003, Miller, 2002, Stewart, 2002)
            anon-WO0118547.85
            eps-15
            (Majumdar et al., 2003)
            eps15
            (Nahm et al., 2013, Jha et al., 2012, Matta et al., 2012, Vijayakrishnan et al., 2010, Venken et al., 2009, Kasprowicz et al., 2008, Koh et al., 2007, Korolchuk et al., 2007, Venken, 2006, Majumdar et al., 2003, Wairkar et al., 2003)
            i31
            (Battersby et al., 2003)
            Name Synonyms
            Epidermal growth factor receptor pathway substrate clone 15
            Eps-15
            Eps15 homologue 1
            (Tang et al., 2005)
            Secondary FlyBase IDs
            • FBgn0061582
            Datasets (0)
            Study focus (0)
            Experimental Role
            Project
            Project Type
            Title
            References (99)