FlyBase Gene Report: Dmel\Eps-15

General Information

Symbol

Dmel\Eps-15

Species

D. melanogaster

Name

Epidermal growth factor receptor pathway substrate clone 15

Annotation Symbol

CG16932

Feature Type

protein_coding_gene

FlyBase ID

FBgn0035060

Gene Model Status

Current

Stock Availability

13 publicly available

Gene Summary

Epidermal growth factor receptor pathway substrate clone 15 (Eps-15) encodes a multi-domain adaptor protein involved in endocytosis. It interacts with several other endocytic factors such as those encoded by shi, stnB, Dap160 and AP-2α. It is enriched at sites of endocytosis and is required for normal synaptic bouton development and vesicle endocytosis during high frequency stimulation. [Date last reviewed: 2018-10-18] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Pathway
Interactive Fly

Also Known As

Eps15

Key Links

Genomic Location

Cytogenetic map

60E1-60E1

Sequence location

2R:24,768,314..24,775,265 [-]

Recombination map

2-107

RefSeq locus

NT_033778 REGION:24768314..24775265

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse GBrowse

Help me choose

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

Gene Ontology (GO) Annotations (9 terms)

Molecular Function (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

enables calcium ion binding

inferred from electronic annotation with InterPro:IPR002048

(assigned by InterPro )

(FlyBase Curators et al., 2004-)

Biological Process (5 terms)

Terms Based on Experimental Evidence (3 terms)

CV Term

Evidence

References

involved_in negative regulation of Notch signaling pathway

inferred from mutant phenotype

(Tang et al., 2005)

involved_in regulation of synaptic assembly at neuromuscular junction

inferred from mutant phenotype

(Koh et al., 2007)

involved_in synaptic vesicle endocytosis

inferred from mutant phenotype

(Koh et al., 2007)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

involved_in endocytosis

inferred from biological aspect of ancestor with PANTHER:PTN000130974

(assigned by GO_Central )

(Gaudet et al., 2011)

involved_in endosomal transport

inferred from biological aspect of ancestor with PANTHER:PTN000130974

(assigned by GO_Central )

(Gaudet et al., 2011)

Cellular Component (3 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

located_in cytoplasm

inferred from high throughput direct assay

(Lee et al., 2014)

colocalizes_with synaptic vesicle

inferred from direct assay

(Koh et al., 2007)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

part_of clathrin coat of coated pit

inferred from biological aspect of ancestor with PANTHER:PTN001677755

(assigned by GO_Central )

(Gaudet et al., 2011)

Gene Group (FlyBase)

Pathway (FlyBase)

Negative Regulators of Notch Signaling Pathway

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

Pathway (FlyBase)

Negative Regulators of Notch Signaling Pathway -: The Notch receptor signaling pathway is activated by the binding of the transmembrane receptor Notch (N) to transmembrane ligands, Dl or Ser, presented on adjacent cells. This results in the proteolytic cleavage of N, releasing the intracellular domain (NICD). NICD translocates into the nucleus, interacting with Su(H) and mam to form a transcription complex, which up-regulates transcription of Notch-responsive genes. Negative regulators of the pathway down-regulate the signal from the sending cell or the response in the receiving cell. (Adapted from FBrf0225731 and FBrf0192604).

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Eps-15 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure New Section

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q9W111)

If you don't see the viewer to the right, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Comments on Gene Model

Gene model reviewed during 5.39

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.52

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Eps-15-RA

FBtr0072435

NM_138121

4421

1253

Eps-15-RB

FBtr0072434

NM_166687

4358

1232

Eps-15-RC

FBtr0072433

NM_166686

3980

1106

Eps-15-RD

FBtr0308684

NM_001259573

3695

792

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

Eps-15-PA

FBpp0072340

134.4

1253

4.82

FBpp0072339

132.1

1232

4.81

FBpp0072338

119.6

1106

4.74

FBpp0300890

85.5

792

4.91

E3CTP7

NP_001246502

AFH08255

Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Eps-15 using the Feature Mapper tool.

External Data

Crossreferences

Linkouts

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that the Fly Cell Atlas project found the gene expressed in that cell type. Darker colors mean that more cells of that cell type express the gene:

low

high

Colorless tiles indicate that there is no scRNAseq data for the gene in that cell type.

Colored tiles in ribbon indicate that expression data (RNA and/or protein) has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

Colored tiles in the ribbon indicate the average RNA expression level of the gene at the indicated stages:

low

high

as determined by RNA-seq (RPKM) using whole organism samples modENCODE, Brown et al., 2014. For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 10 -- 15

presumptive embryonic/larval central nervous system

(Tang et al., 2005)

embryonic stage 16

embryonic/larval central nervous system

(Tang et al., 2005)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

Additional Descriptive Data

Eps-15 is expressed in central brain neuropils. It is uniformly high amongst the different neuropils. In cross sections of the lamina, Eps-15 labelling reveals the outline of photoreceptor R1-R6 terminals, and particularly strong staining was seen at the sites facing the interior of the cartridge. Via immunoelectron microscopy, Eps-15 is seen amongst the vesicle pool in the cytoplasm of the photoreceptors, and often close to the capitate projection (invaginations from surrounding epithelial glial and specialised organelles for the endocytosis of vesicle membrane).

(Hamanaka and Meinertzhagen, 2010)

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in cytoplasm

inferred from high throughput direct assay

(Lee et al., 2014)

colocalizes_with synaptic vesicle

inferred from direct assay

(Koh et al., 2007)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Eps-15 in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Eps-15

View exonic expression by tissue for Dmel\Eps-15

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

37961/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0035060

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0035060

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0035060

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG16932

Flygut - An atlas of the Drosophila adult midgut

FBgn0035060

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 10 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 15 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Eps-15

Transgenic constructs containing regulatory region of Eps-15

Aberrations (Deficiencies and Duplications) ( 6 )

Inferred from experimentation ( 6 )

Gene disrupted in

Df(2R)BSC602

(Christensen et al., 2008.9.3)

Df(2R)BSC604

(Christensen et al., 2008.9.3)

Df(2R)BSC605

(Christensen et al., 2008.9.3)

Df(2R)BSC606

(Christensen et al., 2008.9.3)

Df(2R)BSC856

(Christensen et al., 2009.7.22)

Df(2R)Dll-MP

(Koh et al., 2007)

Inferred from location ( 0 )

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal

lethal (with Df(2R)BSC606)

Eps-15^{CR00466-TG4.0}

lethal | recessive, with Scer\GAL4^- :

Eps-15^EP2513

lethal - all die before end of larval stage (with Df(2R)Dll-MP)

Eps-15^e75

lethal - all die before end of P-stage

Eps-15^Δ29

lethal - all die before end of P-stage (with Eps-15^e75)

Eps-15^Δ29

lethal - all die before end of P-stage (with Eps-15^Δ29)

Eps-15^e75

lethal - all die before end of pupal stage

Eps-15^EP2513

lethal - all die before end of pupal stage (with Df(2R)Dll-MP)

Eps-15^EP2513

lethal - all die before end of pupal stage (with Eps-15²)

Eps-15^EP2513

lethal - all die before end of pupal stage (with Eps-15^EP2513)

Eps-15²

partially lethal - majority die

Eps-15^EP2513

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Eps-15^GD8784

viable, with Scer\GAL4^Mef2.PR

Eps-15^GD8784

viable, with Scer\GAL4^pnr-MD237

Eps-15^GD8784

viable, with Scer\GAL4^tin.CΔ4

Eps-15^GD8784

Sterility

Allele

fertile

Eps-15^EP2513

Other Phenotypes

Allele

abnormal neuroanatomy, with Scer\GAL4^da.G32

Eps-15^VDRC.cUa

abnormal neuroanatomy | larval stage (with Df(2R)Dll-MP)

Eps-15^Δ29

abnormal neuroanatomy | larval stage (with Eps-15^e75)

Eps-15^Δ29

abnormal neuroanatomy | larval stage (with Eps-15^Δ29)

Eps-15^e75

abnormal neurophysiology, with Scer\GAL4^- :

Eps-15^EP2513

abnormal neurophysiology | larval stage (with Df(2R)Dll-MP)

Eps-15^e75

abnormal neurophysiology | larval stage (with Eps-15^e75)

Eps-15^Δ29

abnormal neurophysiology | larval stage (with Eps-15^Δ29)

Eps-15^e75

abnormal pain response | adult stage, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Eps-15^GD8784

some die during pharate adult stage

Eps-15^Δ29

some die during pharate adult stage (with Eps-15^e75)

Eps-15^Δ29

some die during pharate adult stage (with Eps-15^Δ29)

Eps-15^e75

some die during pupal stage

Eps-15^EP2513

some die during pupal stage (with Df(2R)Dll-MP)

Eps-15^EP2513

some die during pupal stage (with Eps-15²)

Eps-15^EP2513

some die during pupal stage (with Eps-15^EP2513)

Eps-15²

some die during third instar larval stage (with Df(2R)Dll-MP)

Eps-15^e75

visible, with Scer\GAL4^en.PU

Eps-15^UAS.cLa

Phenotype manifest in

Allele

bouton (with Df(2R)Dll-MP)

Eps-15^Δ29

bouton (with Eps-15^e75)

Eps-15^Δ29

bouton (with Eps-15^Δ29)

Eps-15^e75

bouton | increased number (with Df(2R)Dll-MP)

Eps-15^Δ29

bouton | increased number (with Eps-15^e75)

Eps-15^Δ29

bouton | increased number (with Eps-15^Δ29)

Eps-15^e75

embryonic/larval neuromuscular junction

Eps-15^EP2513

embryonic/larval neuromuscular junction (with Df(2R)Dll-MP)

embryonic/larval neuromuscular junction (with Eps-15^e75)

Eps-15^Δ29

embryonic/larval neuromuscular junction (with Eps-15^Δ29)

Eps-15^e75

eo sensory structure | increased number, with Scer\GAL4^sca-537.4

Eps-15^UAS.cTa

eye disc, with Scer\GAL4^da.G32

Eps-15^VDRC.cUa

glial cell, with Scer\GAL4^da.G32

Eps-15^VDRC.cUa

macrochaeta, with Scer\GAL4^sca-537.4

macrochaeta (with Df(2R)Dll-MP), with Scer\GAL4^sca-537.4

Eps-15^EP2513

macrochaeta (with Eps-15²), with Scer\GAL4^sca-537.4

Eps-15^EP2513

macrochaeta (with Eps-15^EP2513), with Scer\GAL4^sca-537.4

Eps-15²

macrochaeta | increased number, with Scer\GAL4^sca-537.4

Eps-15^UAS.cTa

tormogen cell, with Scer\GAL4^sca-537.4

tormogen cell (with Df(2R)Dll-MP), with Scer\GAL4^sca-537.4

Eps-15^EP2513

tormogen cell (with Eps-15²), with Scer\GAL4^sca-537.4

Eps-15^EP2513

tormogen cell (with Eps-15^EP2513), with Scer\GAL4^sca-537.4

Eps-15²

tormogen cell | increased number, with Scer\GAL4^sca-537.4

Eps-15^UAS.cTa

wing, with Scer\GAL4^Ilp2.PR

Eps-15^UAS.cLa

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v8.0)

Homo sapiens (Human) (8)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Hsap\EPS15L1

13 of 15

Yes

Hsap\EPS15

11 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Yes

Model Organism Orthologs (via DIOPT v8.0)

Mus musculus (laboratory mouse) (8)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Mmus\Eps15l1

13 of 15

Yes

Mmus\Eps15

10 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Yes

Rattus norvegicus (Norway rat) (7)

Rnor\Eps15l1

11 of 13

Yes

Rnor\Eps15

5 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Xenopus tropicalis (Western clawed frog) (7)

Xtro\eps15l1

8 of 12

Yes

Xtro\eps15

6 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Yes

Danio rerio (Zebrafish) (12)

Drer\eps15l1a

12 of 15

Yes

Drer\eps15

10 of 15

Yes

Drer\eps15l1b

5 of 15

Yes

Drer\si:ch73-140j24.4

2 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Yes

Caenorhabditis elegans (Nematode, roundworm) (3)

Cele\ehs-1

13 of 15

Yes

Cele\R10E11.6

1 of 15

Yes

Cele\reps-1

1 of 15

Arabidopsis thaliana (thale-cress) (2)

Atha\AT1G20760

4 of 9

Yes

Atha\AT1G21630

4 of 9

Yes

Saccharomyces cerevisiae (Brewer's yeast) (6)

Scer\EDE1

10 of 15

Yes

Scer\END3

3 of 15

Yes

Scer\IRS4

3 of 15

Yes

Scer\TAX4

3 of 15

Yes

Scer\PAN1

2 of 15

Scer\TPM1

1 of 15

Schizosaccharomyces pombe (Fission yeast) (5)

Spom\SPBC800.10c

8 of 12

Yes

Spom\ucp8

5 of 12

Yes

Spom\end3

2 of 12

Yes

Spom\SPAC1687.09

2 of 12

Yes

Spom\pan1

1 of 12

Other Organism Orthologs (via OrthoDB)

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v8.0)

Drosophila melanogaster (Fruit fly) (4)

Gene Symbol

Score

Source

Alignment

2 of 10

2 of 10

2 of 10

1 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

cancer, invasive collective cell migration, border cell model

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 2 )

Allele

Disease

Interaction

References

Eps-15^e75

exacerbates Troyer syndrome

modeled by spartin¹

(Nahm et al., 2013)

Eps-15^EP2513

ameliorates severe acute respiratory syndrome

modeled by HCoV-SARS\3a^{UAS.CUHK-W1.EGFP}

(Wong et al., 2005)

Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

EPS15L1; epidermal growth factor receptor pathway substrate 15 like 1

13 of 15

616826

EPS15; epidermal growth factor receptor pathway substrate 15

11 of 15

600051

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

View in Interactions Browser

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Eps-15 - AP-2α

anti bait coimmunoprecipitation, western blot

(Koh et al., 2007, Majumdar et al., 2006)

Eps-15 - Dap160

anti bait coimmunoprecipitation, peptide massfingerprinting, western blot

(Winther et al., 2015, Koh et al., 2007)

Eps-15 - Nak

anti tag coimmunoprecipitation, anti tag western blot

(Peng et al., 2009)

Eps-15 - Ptpmeg

anti tag coimmunoprecipitation, anti tag western blot, Identification by mass spectrometry

(Li et al., 2015)

Eps-15 - ct

anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based

(Rhee et al., 2014)

Eps-15 - hpo

anti tag coimmunoprecipitation, peptide massfingerprinting

(Kwon et al., 2013)

Eps-15 - lqf

pull down, western blot, anti bait coimmunoprecipitation

(Xie et al., 2012, Koh et al., 2007)

Eps-15 - numb

two hybrid, pull down, western blot

(Tang et al., 2005)

Eps-15 - shi

anti bait coimmunoprecipitation, western blot

(Koh et al., 2007, Majumdar et al., 2006)

Eps-15 - spartin

anti tag coimmunoprecipitation, anti tag western blot, pull down

(Nahm et al., 2013)

Eps-15 - stnB

anti bait coimmunoprecipitation, western blot

(Majumdar et al., 2006)

Eps-15 - stumps

two hybrid

(Battersby et al., 2003)

Summary of Genetic Interactions

esyN Network Diagram

esyN Network Key:

Suppression

Enhancement

View in Interactions Browser

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Eps-15

suppressible

Su(H)

(Tang et al., 2005)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

enhanceable

Eps-15

(Tang et al., 2005)

rho

suppressible

Eps-15

(Tjota et al., 2011)

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

63531

DroID - A comprehensive database of gene and protein interactions.

FBgn0035060

MIST (protein-protein) - An integrated Molecular Interaction Database

Eps-15

Pathways

Signaling Pathways (FlyBase)

Negative Regulators of Notch Signaling Pathway -: The Notch receptor signaling pathway is activated by the binding of the transmembrane receptor Notch (N) to transmembrane ligands, Dl or Ser, presented on adjacent cells. This results in the proteolytic cleavage of N, releasing the intracellular domain (NICD). NICD translocates into the nucleus, interacting with Su(H) and mam to form a transcription complex, which up-regulates transcription of Notch-responsive genes. Negative regulators of the pathway down-regulate the signal from the sending cell or the response in the receiving cell. (Adapted from FBrf0225731 and FBrf0192604).

Metabolic Pathways

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0035060

External Data

Linkouts

KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.

Endocytosis

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-107

Cytogenetic map

60E1-60E1

Sequence location

2R:24,768,314..24,775,265 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

60E1-60E1

Limits computationally determined from genome sequence between P{lacW}Mmp1^k04809 and P{EP}Reg-5^EP1065

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

60E-60E

(Lloyd et al., 2000)

Determined by comparing Celera genomic sequence with sequence from BDGP BAC and P1 clones.

(Lloyd et al., 2000)

Experimentally Determined Recombination Data

Location

2-107

(FlyBase, 2016)

2-111.4

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (13)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

29578

y¹ v¹; P{TRiP.JF03257}attP2

17068

w¹¹¹⁸; P{EP}Eps-15^EP2554/CyO

17252

w¹¹¹⁸; P{EP}Eps-15^EP2513/CyO

24888

w^*; P+PBac{XP3.WH3}Eps-15^Δ29/SM6a

39671

w^*; l(2)*^*/CyO; P{UAS-Eps-15.K}3/TM6B, Tb⁺

39672

w^*; l(2)*^*/CyO; P{UAS-Eps-15.ΔDPF}3/TM6B, Tb⁺

33942

y¹ sc^* v¹ sev²¹; P{TRiP.HMS00893}attP2

33987

y¹ sc^* v¹ sev²¹; P{TRiP.HMS00947}attP2

78918

y¹ w^*; TI{CRIMIC.TG4.0}Eps-15^{CR00466-TG4.0}/SM6a

24900

y¹ w^*; Eps-15^e75/CyO, P{GAL4-twi.G}2.2, P{UAS-2xEGFP}AH2.2

VDRC

v106678

P{KK101943}VIE-260B

v19165

w¹¹¹⁸; P{GD8784}v19165

More stocks available...

Genomic Clones (24)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (100)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

SD09478

Other clones

MIP26607

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0035060

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0035060

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

37961

Antibody Information

Laboratory Generated Antibodies

monoclonal

(Halder et al., 2011)

polyclonal

(Koh et al., 2007, Korolchuk et al., 2007)

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for identity of: Eps-15 CG16932

(Verstreken and Bellen, 2000.9.4)

Source for database merge of

Source for merge of: Eps-15 anon-WO0118547.85

(FlyBase, 1996-)

Additional comments

Source for merge of Eps-15 anon-WO0118547.85 was sequence comparison ( date:051113 ).

(FlyBase, 1996-)

Other Comments

dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R⁺ cell morphology.

(Kiger et al., 2003)

Area matching Drosophila EST AI260872 (inverted). This EST has sequence similarity to mouse signalling factor U29156.

(Davies, 2001.3.15)

Origin and Etymology

Discoverer

Etymology

Identification

External Crossreferences and Linkouts ( 56 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

37961

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q9W111

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q9W111

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0035060

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

37961/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0035060

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0035060

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0035060

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG16932

Flygut - An atlas of the Drosophila adult midgut

FBgn0035060

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0035060

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

37961

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC015931

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG16932

MARRVEL_MODEL - MARRVEL (model organism gene)

37961

modMine - A data warehouse for the modENCODE project

FBgn0035060

Linkouts

BioGRID - A database of protein and genetic interactions.

63531

DroID - A comprehensive database of gene and protein interactions.

FBgn0035060

DRSC - Results frm RNAi screens

FBgn0035060

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0035060

FlyMine - An integrated database for Drosophila genomics

FBgn0035060

KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.

Endocytosis

MIST (protein-protein) - An integrated Molecular Interaction Database

Eps-15

Synonyms and Secondary IDs (13)

Reported As

Symbol Synonym

Aa2/2

(Tsai et al., 2019)

CG16932

(Jensen et al., 2013, Ni et al., 2010.12.1, Keleman et al., 2009.8.5, Perkins et al., 2009, Majumdar et al., 2006, Metta et al., 2006, Molnar et al., 2006, Tang et al., 2005, Battersby et al., 2003)

EH1

(Tang et al., 2005)

EPS-15

(Tsai et al., 2019, Hamanaka and Meinertzhagen, 2010, Lloyd et al., 2000)

EPS15

(Wu et al., 2014, Tjota et al., 2011)

anon-WO0118547.85

eps-15

(Majumdar et al., 2003)

eps15

(Nahm et al., 2013, Jha et al., 2012, Matta et al., 2012, Vijayakrishnan et al., 2010, Venken et al., 2009, Kasprowicz et al., 2008, Koh et al., 2007, Korolchuk et al., 2007, Venken, 2006, Majumdar et al., 2003, Wairkar et al., 2003)

i31

(Battersby et al., 2003)

Name Synonyms

Epidermal growth factor receptor pathway substrate clone 15

Eps-15

Eps15 homologue 1

(Tang et al., 2005)

Secondary FlyBase IDs

FBgn0061582

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

References (100)

The following external sites may use different assemblies or annotations than FlyBase.

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