Gene model reviewed during 5.46
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Pex7 using the Feature Mapper tool.
A high level of Pex7 FISH signal is observed in the yolk and at the cortex of the syncytial blastoderm and persists during gastrulation. A time course from 2-22hr AEL was carried out. Roughly 30 min. after the maternal-zygotic transition (MZT) of gene transcription at 1.5 h AEL, a weak, variable signal of Pex7 FISH is observed in the yolk at the center of the embryo. These foci are cortically perinuclear in embryos just before syncytial cellularization and then became ubiquitous following formation of the anterior-posterior axis and cephalization. At 8-12h AEL, Pex7 transcription is restricted to the presumptive supraesophageal ganglion and ventral nerve cord. This pattern persists until 16-20h AEL, when Pex7 mRNA expression is also observed in cells on the cortical layer of the CNS in a pattern most similar to neuroblasts. Immediately prior to hatching (20-22 h AEL), the Pex7 mRNA pattern was observed to be restricted to the anterior half of the presumptive nerve cord and primitive optic lobes, with fewer overall foci observed compared to prior time points.
The Pex7 protein expression pattern is similar to the mRNA pattern. Signal is observed in both the yolk and the pre-cellular syncytium. At the completion of germ band elongation, signal becomes ubiquitous. Comparison to markers shows the folowing: Cells showing strong Pex7 signal are found in cells adjacent to elav expressing cells at 7.3-9.7hr AEL. At 12hr AEL, a pattern is observed characterized by para-segmental repeats of five small distinct branches and two foci adjacent to the ventral nerve cord. Some cells in the extreme anterior/posterior regions also express Pex7 but these do not overlap with the Nrg- or repo-expressing cells. At 16hr AEL, strong signal is observed in a subset of cells on the cortical layer of the CNS. At all subsequent stages, signal is restricted to the ventral nerve cord.
GBrowse - Visual display of RNA-Seq signalsView Dmel\Pex7 in GBrowse 2
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Source for identity of: Pex7 CG6486