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General Information
Symbol
Dmel\Lkb1
Species
D. melanogaster
Name
Lkb1 kinase
Annotation Symbol
CG9374
Feature Type
FlyBase ID
FBgn0038167
Gene Model Status
Stock Availability
Gene Summary
Lkb1 kinase (Lkb1) encodes a serine/threonine kinase that plays a master role for activating the AMP-dependent protein kinase (AMPK) family kinases. It is a well-characterized tumor suppressor and regulates processes like cell polarity, metabolism, apoptosis, and cell growth. [Date last reviewed: 2019-03-14] (FlyBase Gene Snapshot)
Also Known As

PAR-4, dlkb1, STK11

Key Links
Genomic Location
Cytogenetic map
Sequence location
3R:13,676,549..13,679,430 [+]
Recombination map
3-54
RefSeq locus
NT_033777 REGION:13676549..13679430
Sequence
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
Function
GO Summary Ribbons
Gene Ontology (GO) Annotations (25 terms)
Molecular Function (4 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
inferred from physical interaction with FLYBASE:Wdfy2; FB:FBgn0032246
inferred from mutant phenotype
Terms Based on Predictions or Assertions (3 terms)
CV Term
Evidence
References
enables ATP binding
inferred from electronic annotation with InterPro:IPR000719, InterPro:IPR002290, InterPro:IPR017441
(assigned by InterPro )
inferred from electronic annotation with InterPro:IPR039154
(assigned by InterPro )
inferred from sequence or structural similarity with HGNC:11389
inferred from biological aspect of ancestor with PANTHER:PTN002519176
(assigned by GO_Central )
Biological Process (17 terms)
Terms Based on Experimental Evidence (13 terms)
CV Term
Evidence
References
inferred from mutant phenotype
inferred from mutant phenotype
inferred from direct assay
involved_in dorsal closure
inferred from mutant phenotype
inferred from direct assay
inferred from mutant phenotype
inferred from mutant phenotype
inferred from direct assay
inferred from mutant phenotype
inferred from mutant phenotype
inferred from direct assay
Terms Based on Predictions or Assertions (6 terms)
CV Term
Evidence
References
inferred from electronic annotation with InterPro:IPR039154
(assigned by InterPro )
inferred from electronic annotation with InterPro:IPR039154
(assigned by InterPro )
inferred from biological aspect of ancestor with PANTHER:PTN002519176
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN002804554
(assigned by GO_Central )
inferred from sequence or structural similarity with HGNC:11389
inferred from electronic annotation with InterPro:IPR039154
(assigned by InterPro )
Cellular Component (4 terms)
Terms Based on Experimental Evidence (4 terms)
CV Term
Evidence
References
located_in cell cortex
inferred from direct assay
located_in cytoplasm
inferred from direct assay
located_in endosome
inferred from direct assay
located_in nucleus
inferred from direct assay
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
is_active_in cytoplasm
inferred from biological aspect of ancestor with PANTHER:PTN002519176
(assigned by GO_Central )
is_active_in nucleus
inferred from biological aspect of ancestor with PANTHER:PTN002804554
(assigned by GO_Central )
Protein Family (UniProt)
-
Summaries
Gene Snapshot
Lkb1 kinase (Lkb1) encodes a serine/threonine kinase that plays a master role for activating the AMP-dependent protein kinase (AMPK) family kinases. It is a well-characterized tumor suppressor and regulates processes like cell polarity, metabolism, apoptosis, and cell growth. [Date last reviewed: 2019-03-14]
Gene Group (FlyBase)
CALCIUM/CALMODULIN-DEPENDENT PROTEIN KINASES -
The Calcium/Calmodulin-dependent kinase family are serine/threonine kinases which, in general, prefer substrates containing basic residues. Many, but not all, members are regulated by calcium or calmodulin. (Adapted from FBrf0132098).
Summary (Interactive Fly)

Par-4 kinase - required for the early A-P polarity of the oocyte and for the repolarization of the oocyte cytoskeleton that defines the embryonic A-P axis

Gene Model and Products
Number of Transcripts
9
Number of Unique Polypeptides
1

Please see the JBrowse view of Dmel\Lkb1 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model

Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.

Gene model reviewed during 5.47

Sequence Ontology: Class of Gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
FBtr0082808
2428
567
FBtr0082809
2397
567
FBtr0082810
2391
567
FBtr0082811
2422
567
FBtr0082812
2553
567
FBtr0082813
2407
567
FBtr0082814
2401
567
FBtr0082815
2403
567
FBtr0301488
2487
567
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
FBpp0082276
62.9
567
5.58
FBpp0082277
62.9
567
5.58
FBpp0082278
62.9
567
5.58
FBpp0082279
62.9
567
5.58
FBpp0082280
62.9
567
5.58
FBpp0082281
62.9
567
5.58
FBpp0082282
62.9
567
5.58
FBpp0082283
62.9
567
5.58
FBpp0290703
62.9
567
5.58
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Lkb1 using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
organism

Comment: maternally deposited

Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
located_in cell cortex
inferred from direct assay
located_in cytoplasm
inferred from direct assay
located_in endosome
inferred from direct assay
located_in nucleus
inferred from direct assay
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Lkb1 in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
EMBL-EBI Single Cell Expression Atlas
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 9 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 33 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of Lkb1
Transgenic constructs containing regulatory region of Lkb1
Aberrations (Deficiencies and Duplications) ( 3 )
Inferred from experimentation ( 3 )
Inferred from location ( 0 )
Alleles Representing Disease-Implicated Variants
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
Sterility
Allele
Other Phenotypes
Allele
Phenotype manifest in
Allele
centrosome & larval neuroblast (with Df(3R)urd)
oocyte & microtubule | germ-line clone
spindle & larval neuroblast (with Df(3R)urd)
Orthologs
Human Orthologs (via DIOPT v8.0)
Homo sapiens (Human) (2)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
12 of 15
Yes
Yes
2  
1 of 15
No
No
Model Organism Orthologs (via DIOPT v8.0)
Mus musculus (laboratory mouse) (1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
12 of 15
Yes
Yes
Rattus norvegicus (Norway rat) (2)
9 of 13
Yes
Yes
1 of 13
No
No
Xenopus tropicalis (Western clawed frog) (2)
6 of 12
Yes
Yes
1 of 12
No
No
Danio rerio (Zebrafish) (3)
7 of 15
Yes
Yes
1 of 15
No
No
1 of 15
No
No
Caenorhabditis elegans (Nematode, roundworm) (1)
11 of 15
Yes
Yes
Arabidopsis thaliana (thale-cress) (0)
No records found.
Saccharomyces cerevisiae (Brewer's yeast) (2)
1 of 15
Yes
No
1 of 15
Yes
No
Schizosaccharomyces pombe (Fission yeast) (1)
2 of 12
Yes
Yes
Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG09190736 )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila suzukii
Spotted wing Drosophila
Drosophila suzukii
Spotted wing Drosophila
Drosophila simulans
Drosophila sechellia
Drosophila erecta
Drosophila yakuba
Drosophila ananassae
Drosophila pseudoobscura pseudoobscura
Drosophila persimilis
Drosophila willistoni
Drosophila virilis
Drosophila mojavensis
Drosophila grimshawi
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091504W7 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Glossina morsitans
Tsetse fly
Lucilia cuprina
Australian sheep blowfly
Mayetiola destructor
Hessian fly
Aedes aegypti
Yellow fever mosquito
Anopheles darlingi
American malaria mosquito
Anopheles gambiae
Malaria mosquito
Anopheles gambiae
Malaria mosquito
Culex quinquefasciatus
Southern house mosquito
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W04BW )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Danaus plexippus
Monarch butterfly
Heliconius melpomene
Postman butterfly
Apis florea
Little honeybee
Apis mellifera
Western honey bee
Bombus impatiens
Common eastern bumble bee
Bombus terrestris
Buff-tailed bumblebee
Linepithema humile
Argentine ant
Megachile rotundata
Alfalfa leafcutting bee
Nasonia vitripennis
Parasitic wasp
Dendroctonus ponderosae
Mountain pine beetle
Tribolium castaneum
Red flour beetle
Pediculus humanus
Human body louse
Rhodnius prolixus
Kissing bug
Cimex lectularius
Bed bug
Acyrthosiphon pisum
Pea aphid
Acyrthosiphon pisum
Pea aphid
Zootermopsis nevadensis
Nevada dampwood termite
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X048H )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strigamia maritima
European centipede
Ixodes scapularis
Black-legged tick
Ixodes scapularis
Black-legged tick
Stegodyphus mimosarum
African social velvet spider
Stegodyphus mimosarum
African social velvet spider
Stegodyphus mimosarum
African social velvet spider
Tetranychus urticae
Two-spotted spider mite
Daphnia pulex
Water flea
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G0BNP )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strongylocentrotus purpuratus
Purple sea urchin
Ciona intestinalis
Vase tunicate
Paralogs
Paralogs (via DIOPT v8.0)
Drosophila melanogaster (Fruit fly) (8)
2 of 10
1 of 10
1 of 10
1 of 10
1 of 10
1 of 10
1 of 10
1 of 10
Human Disease Associations
FlyBase Human Disease Model Reports
Disease Model Summary Ribbon
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 4 )
Potential Models Based on Orthology ( 3 )
Modifiers Based on Experimental Evidence ( 3 )
Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)
Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
Functional Complementation Data
Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
Interactions
Summary of Physical Interactions
esyN Network Diagram
Show neighbor-neighbor interactions:
Select Layout:
Legend:
Protein
RNA
Selected Interactor(s)
Interactions Browser

Please see the Physical Interaction reports below for full details
protein-protein
Physical Interaction
Assay
References
Summary of Genetic Interactions
esyN Network Diagram
esyN Network Key:
Suppression
Enhancement

Please look at the allele data for full details of the genetic interactions
Starting gene(s)
Interaction type
Interacting gene(s)
Reference
Starting gene(s)
Interaction type
Interacting gene(s)
Reference
External Data
Linkouts
DroID - A comprehensive database of gene and protein interactions.
InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
MIST (genetic) - An integrated Molecular Interaction Database
MIST (protein-protein) - An integrated Molecular Interaction Database
Pathways
Signaling Pathways (FlyBase)
Metabolic Pathways
External Data
Linkouts
KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.
Genomic Location and Detailed Mapping Data
Chromosome (arm)
3R
Recombination map
3-54
Cytogenetic map
Sequence location
3R:13,676,549..13,679,430 [+]
FlyBase Computed Cytological Location
Cytogenetic map
Evidence for location
87F7-87F9
Limits computationally determined from genome sequence between P{lacW}B52s2249&P{lacW}flflL4179 and P{PZ}flfl01949
Experimentally Determined Cytological Location
Cytogenetic map
Notes
References
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Stocks and Reagents
Stocks (12)
Genomic Clones (24)
cDNA Clones (145)
 

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced
BDGP DGC clones
Other clones
Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

cDNA Clones, End Sequenced (ESTs)
RNAi and Array Information
Linkouts
DRSC - Results frm RNAi screens
GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
Antibody Information
Laboratory Generated Antibodies
Commercially Available Antibodies
 
Other Information
Relationship to Other Genes
Source for database identify of

Source for identity of: lkb1 CG9374

Source for identity of: Lkb1 lkb1

Source for database merge of

Source for merge of: CG9374 anon- EST:Posey135

Additional comments
Other Comments

The kinase activity of lkb1 is essential for its genetic interaction with Sln.

BrdU incorporation analysis and phosphohistone H3 immunostaining reveal that lkb1 expression does not affect G1/S or G2/M cell cycle progression.

lkb1 induces apoptotic cell death but not cell cycle arrest.

lkb1 regulates organ size of the central nervous system by inducing developmental apoptosis during embryogenesis.

lkb1 is upstream of grim, rpr, and W in the pro-apoptotic pathway.

p53 is dispensable for lkb1-dependent apoptosis and vice versa.

lkb1-induced apoptosis is caspase-dependent.

The tumor-suppressing activity of lkb1 is unrelated to the negative regulation of cell growth control.

When dsRNA constructs are made and transiently transfected into S2 cells in RNAi experiments, a whole range of mitotic abnormalities, centrosome abnormalities and spindle abnormalities are seen.

dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.

Identification: lkb1 was identified in a genetic screen for germ-line clone mutants that disrupt the localization of the product of the transgene stauαTub67C.T:Avic\GFP-m6 in the oocyte.

lkb1 is required for early A-P polarity of the oocyte and for the repolarization of the oocyte cytoskeleton that defines the embryonic A-P axis.

Identification: in a germline clone screen for mutants that are defective in localisation of an Avic\GFP-stau marker in living oocytes. 2 alleles of lkb1 have been identified in the screen.

Origin and Etymology
Discoverer
Etymology
Identification
External Crossreferences and Linkouts ( 65 )
Sequence Crossreferences
NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
Other crossreferences
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
EMBL-EBI Single Cell Expression Atlas
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
KEGG Genes - Molecular building blocks of life in the genomic space.
MARRVEL_MODEL
modMine - A data warehouse for the modENCODE project
SignaLink - A signaling pathway resource with multi-layered regulatory networks.
Linkouts
DroID - A comprehensive database of gene and protein interactions.
DRSC - Results frm RNAi screens
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
FlyMine - An integrated database for Drosophila genomics
Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.
MIST (genetic) - An integrated Molecular Interaction Database
MIST (protein-protein) - An integrated Molecular Interaction Database
Synonyms and Secondary IDs (16)
Reported As
Symbol Synonym
LKB1/PEUTZ JEGHERS KINASE
anon-EST:Posey135
par-4
Name Synonyms
Lkb1 kinase
lkb1
Secondary FlyBase IDs
  • FBgn0027757
  • FBgn0045844
Datasets (0)
Study focus (0)
Experimental Role
Project
Project Type
Title
References (119)