FlyBase Gene Report: Dmel\HtrA2

General Information

Symbol

Dmel\HtrA2

Species

D. melanogaster

Name

HTRA2-related serine protease

Annotation Symbol

CG8464

Feature Type

protein_coding_gene

FlyBase ID

FBgn0038233

Gene Model Status

Current

Stock Availability

7 publicly available

Gene Snapshot

Contributions welcome

Other Summaries

Alliance
Auto summary
Gene Group
UniProtKB
Interactive Fly

Also Known As

omi, dOmi, DmHtrA2

Key Links

Genomic Location

Cytogenetic map

88C3-88C3

Sequence location

3R:14,509,049..14,511,712 [-]

Recombination map

3-55

RefSeq locus

NT_033777 REGION:14509049..14511712

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse GBrowse

Help me choose

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

Gene Ontology (GO) Annotations (13 terms)

Molecular Function (3 terms)

Terms Based on Experimental Evidence (3 terms)

CV Term

Evidence

References

enables peptidase activity

inferred from direct assay

(Tain et al., 2009, Khan et al., 2008)

enables protein binding

inferred from physical interaction with FLYBASE:rho-7; FB:FBgn0033672

(Whitworth et al., 2008)

inferred from physical interaction with UniProtKB:Q24306

(assigned by UniProt )

(Khan et al., 2008, Igaki et al., 2007)

enables serine-type endopeptidase activity

inferred from direct assay

(Challa et al., 2007)

inferred from mutant phenotype

(assigned by UniProt )

(Khan et al., 2008, Igaki et al., 2007)

Terms Based on Predictions or Assertions (0 terms)

Biological Process (6 terms)

Terms Based on Experimental Evidence (6 terms)

CV Term

Evidence

References

involved_in ectopic germ cell programmed cell death

inferred from mutant phenotype

(Yacobi-Sharon et al., 2013)

involved_in mitochondrion organization

inferred from mutant phenotype

(Tain et al., 2009)

involved_in positive regulation of apoptotic process

inferred from mutant phenotype

(Challa et al., 2007)

involved_in positive regulation of cysteine-type endopeptidase activity

inferred from direct assay

(Challa et al., 2007)

involved_in regulation of apoptotic process

inferred from mutant phenotype

(assigned by UniProt )

(Khan et al., 2008, Challa et al., 2007, Igaki et al., 2007)

involved_in spermatogenesis

inferred from mutant phenotype

(Yun et al., 2008)

Terms Based on Predictions or Assertions (0 terms)

Cellular Component (4 terms)

Terms Based on Experimental Evidence (4 terms)

CV Term

Evidence

References

located_in cytosol

inferred from direct assay

(Challa et al., 2007)

located_in mitochondrial intermembrane space

inferred from direct assay

(assigned by UniProt )

(Khan et al., 2008, Igaki et al., 2007)

inferred from direct assay

(Challa et al., 2007)

located_in mitochondrion

inferred from direct assay

(Khan et al., 2008, Whitworth et al., 2008)

located_in Nebenkern

inferred from direct assay

(Yun et al., 2008)

Terms Based on Predictions or Assertions (0 terms)

Gene Group (FlyBase)

RHG PROTEINS

S1C SERINE PEPTIDASES

Protein Family (UniProt)

Belongs to the peptidase S1C family. (Q9VFJ3)

Protein Signatures (InterPro)

Summaries

Gene Group (FlyBase)

RHG PROTEINS -: RHG proteins possess a 12 amino acid RHG motif. This motif, also known as the IAP-binding motif (IBM), interacts with the BIR domain of Inhibitor of apoptosis proteins (IAPs) and relieving IAP-mediated caspase inhibition. Over-expression of individual RHG proteins is sufficient to induce apoptosis. (Adapted from FBrf0225510).
S1C SERINE PEPTIDASES -: S1C serine peptidases belong to MEROPS family S1, subfamily C. It is a relatively small family in Drosophila melanogaster. (Adapted from FBrf0239469.)

Protein Function (UniProtKB)

Serine protease that shows proteolytic activity against a non-specific substrate beta-casein. Promotes or induces cell death either by direct binding to and inhibition of BIRC proteins (also called inhibitor of apoptosis proteins, IAPs), leading to an increase in caspase activity, or by a BIRC inhibition-independent, caspase-independent and serine protease activity-dependent mechanism. Can antagonize antiapoptotic activity of th by directly inducing the degradation of th.

(UniProt, Q9VFJ3)

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\HtrA2 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Comments on Gene Model

Gene model reviewed during 5.44

Gene model reviewed during 5.48

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

HtrA2-RA

FBtr0082987

NM_142109

1415

422

HtrA2-RB

FBtr0330045

NM_001275636

2512

422

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

HtrA2-PA

FBpp0082446

46.0

422

10.00

NP_650366

AAF55062

HtrA2-PB

FBpp0303078

46.0

422

10.00

NP_001262565

AGB95946

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

422 aa isoforms: HtrA2-PA, HtrA2-PB

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Subunit Structure (UniProtKB)

Interacts with th/DIAP1 (via BIR 2 domain).

(UniProt, Q9VFJ3)

Post Translational Modification

Ubiquitinated by th, thereby antagonizing induced cell death.

Autoproteolytically cleaved into active forms in the mitochondria.

(UniProt, Q9VFJ3)

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\HtrA2 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

HtrA2_1

Linkouts

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 7 -- 8

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 9 -- 10

organism | faint | ubiquitous

(Fisher et al., 2012)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in cytosol

inferred from direct assay

(Challa et al., 2007)

located_in mitochondrial intermembrane space

inferred from direct assay

(assigned by UniProt )

(Khan et al., 2008, Igaki et al., 2007)

inferred from direct assay

(Challa et al., 2007)

located_in mitochondrion

inferred from direct assay

(Khan et al., 2008, Whitworth et al., 2008)

located_in Nebenkern

inferred from direct assay

(Yun et al., 2008)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\HtrA2 in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\HtrA2

View exonic expression by tissue for Dmel\HtrA2

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0038233

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0038233

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG8464-RA

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG8464

Flygut - An atlas of the Drosophila adult midgut

FBgn0038233

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\HtrA2

Stages(s) 1-3
Stages(s) 7-8
Stages(s) 9-10
Stages(s) 11-12
Stages(s) 13-16

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 6 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 29 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of HtrA2

Transgenic constructs containing regulatory region of HtrA2

Aberrations (Deficiencies and Duplications) ( 3 )

Inferred from experimentation

Gene partially disrupted in

Df(3R)HtrA2^Δ1

(Tain et al., 2009)

Gene disrupted in

Df(3R)HtrA2^Df1

(Yacobi-Sharon et al., 2013)

Df(3R)HtrA2^Df2

(Yacobi-Sharon et al., 2013)

Inferred from location

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

partially lethal - majority die

HtrA2^NSO

viable

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

HtrA2^GD13932

viable, with Scer\GAL4^da.G32

HtrA2^dsRNA.UAS

viable, with Scer\GAL4^Mef2.PR

HtrA2^GD13932

viable, with Scer\GAL4^pnr-MD237

HtrA2^GD13932

viable, with Scer\GAL4^tin.CΔ4

HtrA2^GD13932

viable, with Scer\GAL4^Tub.PU

HtrA2^{dsRNA.UAS.cYa}

viable (with Df(3R)ED5644)

HtrA2^NSO

Sterility

Allele

female fertile

fertile, with Scer\GAL4^da.G32

HtrA2^dsRNA.UAS

male fertile

male sterile

male sterile (with Df(3R)ED5644)

male sterile (with HtrA2^NSO)

HtrA2^V110E

male sterile (with HtrA2^V110E)

HtrA2^NSO

Other Phenotypes

Allele

abnormal locomotor behavior

HtrA2^Δ1

chemical resistant | adult stage, with Scer\GAL4^da.G32

HtrA2^dsRNA.UAS

chemical sensitive

HtrA2^Δ1

decreased cell death (with Df(3R)HtrA2^Df1)

HtrA2^IFD

decreased cell death (with Df(3R)HtrA2^Df2)

HtrA2^IFD

decreased cell death | dominant

HtrA2^IFD

flightless

HtrA2^Δ1

radiation resistant | third instar larval stage, with Scer\GAL4^da.G32

HtrA2^dsRNA.UAS

short lived

HtrA2^Δ1

short lived | heat sensitive, with Scer\GAL4^tin.CΔ4

HtrA2^GD13932

visible

HtrA2^ΔPDZ.GMR

visible, with Scer\GAL4^GMR.PF

visible | heat sensitive

HtrA2^GMR.PK

visible | heat sensitive, with Scer\GAL4^GMR.PF

HtrA2^UAS.cYa

visible | pupal stage, with Scer\GAL4^da.G32

HtrA2^UAS.cIa

Phenotype manifest in

Allele

eye

HtrA2^ΔPDZ.GMR

eye, with Scer\GAL4^GMR.PF

eye | heat sensitive

HtrA2^GMR.PK

eye | heat sensitive, with Scer\GAL4^GMR.PF

HtrA2^UAS.cYa

eye | pupal stage, with Scer\GAL4^da.G32

HtrA2^UAS.cIa

indirect flight muscle, with Scer\GAL4^Mef2.PR

investment cone (with Df(3R)ED5644)

HtrA2^NSO

male germline cell

male germline cell (with Df(3R)HtrA2^Df1)

HtrA2^IFD

male germline cell (with Df(3R)HtrA2^Df2)

HtrA2^IFD

mitochondrion & indirect flight muscle

HtrA2^Δ1

spermatid cyst (with Df(3R)ED5644)

HtrA2^NSO

spermatogonial cyst

spermatozoon

HtrA2^Δ1

Orthologs

Downloads

Download All DIOPT Orthologs

Download All OrthoDB Orthologs

Human Orthologs (via DIOPT v8.0)

Homo sapiens (Human) (7)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Hsap\HTRA2

13 of 15

Yes

Hsap\HTRA1

11 of 15

Yes

Hsap\HTRA3

10 of 15

Yes

Hsap\HTRA4

6 of 15

Yes

Hsap\PSMD9

1 of 15

Hsap\TYR

1 of 15

Yes

Hsap\TYSND1

1 of 15

Model Organism Orthologs (via DIOPT v8.0)

Mus musculus (laboratory mouse) (6)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Mmus\Htra2

13 of 15

Yes

Mmus\Htra1

10 of 15

Yes

Mmus\Htra3

8 of 15

Yes

Mmus\Htra4

8 of 15

Yes

Mmus\Psmd9

1 of 15

Mmus\Tysnd1

1 of 15

Rattus norvegicus (Norway rat) (6)

Rnor\Htra2

12 of 13

Yes

Rnor\Htra1

8 of 13

Yes

Rnor\Htra3

7 of 13

Yes

Rnor\Htra4

5 of 13

Yes

Rnor\Psmd9

1 of 13

Rnor\Tysnd1

1 of 13

Xenopus tropicalis (Western clawed frog) (6)

Xtro\htra1

8 of 12

Yes

Xtro\htra4

8 of 12

Yes

Xtro\htra2

2 of 12

Yes

Xtro\htra3

2 of 12

Yes

Xtro\psmd9

1 of 12

Xtro\tysnd1

1 of 12

Danio rerio (Zebrafish) (53)

Drer\htra1b

10 of 15

Yes

Drer\htra1a

9 of 15

Yes

Drer\si:busm1-sl7.7

9 of 15

Yes

Drer\si:dkey-10b15.8

9 of 15

Yes

Drer\si:dkey-84o3.7

9 of 15

Yes

Drer\htra4

8 of 15

Yes

Drer\si:dkey-19p15.4

8 of 15

Yes

Drer\si:dkey-33c12.12

8 of 15

Yes

Drer\htra3a

7 of 15

Yes

Drer\htra3b

7 of 15

Yes

Drer\LOC100331049

7 of 15

Yes

Drer\si:dkey-112g5.13

7 of 15

Yes

Drer\zgc:174193

7 of 15

Yes

Drer\LOC793110

6 of 15

Yes

Drer\LOC797799

6 of 15

Yes

Drer\si:dkey-33c12.10

6 of 15

Yes

Drer\si:dkey-84o3.2

6 of 15

Yes

Drer\si:dkey-112g5.16

6 of 15

Yes

Drer\si:dkey-33c12.13

5 of 15

Yes

Drer\LOC799006

4 of 15

Yes

Drer\LOC560917

3 of 15

Yes

Drer\LOC793521

3 of 15

Yes

Drer\LOC100331451

3 of 15

Yes

Drer\LOC103909414

3 of 15

Yes

Drer\LOC110437759

3 of 15

Yes

Drer\LOC562245

2 of 15

Yes

Drer\LOC799834

2 of 15

Yes

Drer\LOC100149563

2 of 15

Yes

Drer\LOC100330882

2 of 15

Yes

Drer\LOC100333082

2 of 15

Yes

Drer\LOC100535800

2 of 15

Yes

Drer\LOC101883763

2 of 15

Yes

Drer\LOC103911769

2 of 15

Yes

Drer\LOC108179121

2 of 15

Yes

Drer\LOC110437735

2 of 15

Yes

Drer\LOC110437830

2 of 15

Yes

Drer\LOC110437862

2 of 15

Yes

Drer\LOC110438860

2 of 15

Yes

Drer\LOC110438863

2 of 15

Yes

Drer\LOC110438864

2 of 15

Yes

Drer\LOC110438948

2 of 15

Yes

Drer\LOC110438950

2 of 15

Yes

Drer\LOC110438955

2 of 15

Yes

Drer\LOC110438956

2 of 15

Yes

Drer\LOC110438965

2 of 15

Yes

Drer\si:dkey-33c12.11

2 of 15

Yes

Drer\LOC559585

1 of 15

Yes

Drer\LOC100149285

1 of 15

Yes

Drer\LOC110437853

1 of 15

Yes

Drer\LOC110437868

1 of 15

Yes

Drer\LOC110438960

1 of 15

Yes

Drer\LOC110438961

1 of 15

Yes

Drer\psmd9

1 of 15

Caenorhabditis elegans (Nematode, roundworm) (1)

Cele\psmd-9

1 of 15

Yes

Arabidopsis thaliana (thale-cress) (6)

Atha\DEG14

7 of 9

Yes

Atha\DEG1

2 of 9

Yes

Atha\DEG8

2 of 9

Yes

Atha\DEG5

1 of 9

Yes

Atha\DEG6

1 of 9

Yes

Atha\DEG16

1 of 9

Yes

Saccharomyces cerevisiae (Brewer's yeast) (2)

Scer\NMA111

6 of 15

Yes

Scer\NAS2

1 of 15

Schizosaccharomyces pombe (Fission yeast) (2)

Spom\SPAC23G3.12c

4 of 12

Yes

Spom\SPBC1685.05

2 of 12

Yes

Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG091908Z4 )

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila suzukii

Spotted wing Drosophila

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA21097

Drosophila persimilis

Dper\GL24014

Drosophila willistoni

Dwil\GK12147

Drosophila virilis

Dvir\GJ24448

Drosophila mojavensis

Dmoj\GI24802

Drosophila grimshawi

Dgri\GH13631

Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091508S2 )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Musca domestica

House fly

Mdoa\17020097

Glossina morsitans

Tsetse fly

Gmor\GMOY006773

Lucilia cuprina

Australian sheep blowfly

Lcup\FF38_06301

Mayetiola destructor

Hessian fly

Mdes\Mdes008009

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL006373

Anopheles darlingi

American malaria mosquito

Adar\ADAC007338

Anopheles gambiae

Malaria mosquito

Agam\AGAP000240

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ019291

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ005160

Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W047S )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Bombyx mori

Silkmoth

Bmor\BGIBMGA002317

Bombyx mori

Silkmoth

Bmor\BGIBMGA002318

Danaus plexippus

Monarch butterfly

Dple\DPOGS208208-PA

Danaus plexippus

Monarch butterfly

Dple\DPOGS208209-PA

Heliconius melpomene

Postman butterfly

Hmel\HMEL002358

Apis florea

Little honeybee

Aflo\2747350788

Apis florea

Little honeybee

Aflo\3153106676

Apis mellifera

Western honey bee

Amel\GB52923

Apis mellifera

Western honey bee

Amel\HtrA2

Bombus impatiens

Common eastern bumble bee

Bimp\BIMP18011

Bombus impatiens

Common eastern bumble bee

Bimp\BIMP22581

Bombus terrestris

Buff-tailed bumblebee

Bter\15210725

Bombus terrestris

Buff-tailed bumblebee

Bter\15210720

Linepithema humile

Argentine ant

Lhum\LH18248

Linepithema humile

Argentine ant

Lhum\LH18202

Megachile rotundata

Alfalfa leafcutting bee

Mrot\MROTU:000a66

Megachile rotundata

Alfalfa leafcutting bee

Mrot\MROTU:000a5e

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG000369

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG003441

Dendroctonus ponderosae

Mountain pine beetle

Dpod\YQE_10852

Dendroctonus ponderosae

Mountain pine beetle

Dpod\YQE_07422

Tribolium castaneum

Red flour beetle

Tcas\TC012681

Tribolium castaneum

Red flour beetle

Tcas\TC003009

Tribolium castaneum

Red flour beetle

Tcas\TC003010

Pediculus humanus

Human body louse

Phum\PHUM228500

Pediculus humanus

Human body louse

Phum\PHUM529710

Rhodnius prolixus

Kissing bug

Rpro\RPRC014366

Rhodnius prolixus

Kissing bug

Rpro\RPRC014391

Rhodnius prolixus

Kissing bug

Rpro\RPRC009595

Rhodnius prolixus

Kissing bug

Rpro\RPRC014390

Cimex lectularius

Bed bug

Clec\CLEC000674

Cimex lectularius

Bed bug

Clec\CLEC002152

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI007786

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_02435

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_02436

Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X0DQL )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strigamia maritima

European centipede

Smar\SMAR000386

Strigamia maritima

European centipede

Smar\SMAR003311

Ixodes scapularis

Black-legged tick

Isca\ISCW020167

Ixodes scapularis

Black-legged tick

Isca\ISCW007523

Stegodyphus mimosarum

African social velvet spider

Smim\KFM57881.1

Stegodyphus mimosarum

African social velvet spider

Smim\KFM64276.1

Tetranychus urticae

Two-spotted spider mite

Turt\tetur04g01740

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_51732

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_307522

Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G0LXR )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Nagk_1

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Omi/HtrA2

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Nagk

Ciona intestinalis

Vase tunicate

Cint\ENSCING00000025030

Ciona intestinalis

Vase tunicate

Cint\ENSCING00000009973

Gallus gallus

Domestic chicken

Ggal\HTRA1

Gallus gallus

Domestic chicken

Ggal\ENSGALG00000027539

Gallus gallus

Domestic chicken

Ggal\HTRA3

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v8.0)

Drosophila melanogaster (Fruit fly) (3)

Gene Symbol

Score

Source

Alignment

1 of 10

1 of 10

1 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

Parkinson disease 13, susceptibility to

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 2 )

Allele

Disease

Evidence

References

HtrA2^ΔPDZ.GMR

model of Parkinson's disease

CEA

(Khan et al., 2008)

HtrA2^Δ1

model of Parkinson's disease

CEA

(Tain et al., 2009)

Potential Models Based on Orthology ( 4 )

Human Ortholog

Disease

Evidence

References

HTRA2; HtrA serine peptidase 2

model of 3-methylglutaconic aciduria type 8

IEA

(FlyBase, 2019-)

HTRA1; HtrA serine peptidase 1

IEA

(FlyBase, 2019-)

model of CADASIL 2

IEA

(FlyBase, 2019-)

model of CADASIL

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 5 )

Allele

Disease

Interaction

References

HtrA2^ΔPDZ.GMR

model of Parkinson's disease

is ameliorated by Diap1^GMR.PH

(Khan et al., 2008)

HtrA2^UASp.cTa

ameliorates Parkinson's disease 6

modeled by Pink1^B9

(Tain et al., 2009)

HtrA2^UAS.cYa

ameliorates Parkinson's disease 1

modeled by Hsap\SNCA^UAS.cFa

(Chung et al., 2020)

HtrA2^HM05030

exacerbates Parkinson's disease 1

modeled by Hsap\SNCA^UAS.cFa

(Chung et al., 2020)

HtrA2^DF1

DOES NOT exacerbate testicular cancer

modeled by p53^GD11134

(Napoletano et al., 2017)

Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

HTRA2; HtrA serine peptidase 2

13 of 15

606441

3-methylglutaconic aciduria type 8

HTRA1; HtrA serine peptidase 1

11 of 15

602194

HTRA3; HtrA serine peptidase 3

10 of 15

608785

HTRA4; HtrA serine peptidase 4

6 of 15

610700

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

View in Interactions Browser

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

HtrA2 - CG4461

experimental knowledge based

(Guruharsha et al., 2011)

HtrA2 - Diap1

pull down, molecular weight estimation by staining, autoradiography, western blot, protease assay

(Khan et al., 2008, Challa et al., 2007)

HtrA2 - Diap2

pull down, autoradiography, protease assay

(Khan et al., 2008)

HtrA2 - Opa1

anti bait coimmunoprecipitation, peptide massfingerprinting

(Banerjee and Chinthapalli, 2014)

HtrA2 - Pink1

anti tag coimmunoprecipitation, anti tag western blot

(Whitworth et al., 2008)

HtrA2 - rho-7

anti tag coimmunoprecipitation, anti tag western blot

(Whitworth et al., 2008)

Summary of Genetic Interactions

esyN Network Diagram

esyN Network Key:

Suppression

Enhancement

View in Interactions Browser

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

HtrA2

suppressible

Diap1

(Khan et al., 2008)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

park

enhanceable

HtrA2

(Tain et al., 2009)

Pink1

suppressible

HtrA2

(Tain et al., 2009, Whitworth et al., 2008)

Pink1

enhanceable

HtrA2

(Whitworth et al., 2008, Yun et al., 2008)

Pink1|park

suppressible

HtrA2

(Whitworth et al., 2008)

rho-7

suppressible

HtrA2

(Whitworth et al., 2008)

rho-7

enhanceable

HtrA2

(Whitworth et al., 2008)

YME1L

suppressible

HtrA2

(Qi et al., 2016)

External Data

Subunit Structure (UniProtKB)

Interacts with th/DIAP1 (via BIR 2 domain).

(UniProt, Q9VFJ3 )

Linkouts

BioGRID - A database of protein and genetic interactions.

66831

DroID - A comprehensive database of gene and protein interactions.

FBgn0038233

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

41756

MIST (genetic) - An integrated Molecular Interaction Database

HtrA2

MIST (protein-protein) - An integrated Molecular Interaction Database

HtrA2

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

External Data

Linkouts

KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-55

Cytogenetic map

88C3-88C3

Sequence location

3R:14,509,049..14,511,712 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

88C3-88C3

Limits computationally determined from genome sequence between P{PZ}cv-c⁰⁶⁹⁵¹ and P{PZ}put¹⁰⁴⁶⁰

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

3-55

(FlyBase, 2016)

3-54.1

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (7)

Bloomington

28544

y¹ v¹; P{TRiP.HM05030}attP2

76446

y¹ sc^* v¹ sev²¹; P{TKO.GS00619}attP40

78250

y¹ sc^* v¹ sev²¹; P{TOE.GS01465}attP40

55165

y¹ sc^* v¹ sev²¹; P{TRiP.HMC03843}attP40

VDRC

v24104

w¹¹¹⁸; P{GD13932}v24104

v24106

w¹¹¹⁸; P{GD13932}v24106

v318584

PBac{fTRG00524.sfGFP-TVPTBF}VK00033

Genomic Clones (16)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (1)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

AT14262

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0038233

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0038233

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

41756

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Khan et al., 2008, Igaki et al., 2007)

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for identity of: HtrA2 CG8464

(Igaki et al., 2003.6.16)

Source for database merge of

Additional comments

Other Comments

dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R⁺ cell morphology.

(Kiger et al., 2003)

Origin and Etymology

Discoverer

Etymology

Identification

External Crossreferences and Linkouts ( 41 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

41756

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

Q9VFJ3

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

A0A0B4KG52

Other crossreferences

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0038233

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0038233

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

HtrA2_1

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG8464

Flygut - An atlas of the Drosophila adult midgut

FBgn0038233

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

41756

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG8464

modMine - A data warehouse for the modENCODE project

FBgn0038233

Linkouts

BioGRID - A database of protein and genetic interactions.

66831

DroID - A comprehensive database of gene and protein interactions.

FBgn0038233

DRSC - Results frm RNAi screens

FBgn0038233

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0038233

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG8464-RA

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0038233

FlyMine - An integrated database for Drosophila genomics

FBgn0038233

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

41756

KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.

MIST (genetic) - An integrated Molecular Interaction Database

HtrA2

MIST (protein-protein) - An integrated Molecular Interaction Database

HtrA2

Synonyms and Secondary IDs (15)

Reported As

Symbol Synonym

CG8464

(Jiang, 2019.3.1, Banerjee and Chinthapalli, 2014, Ni et al., 2010.12.1, Perkins et al., 2009, Tain et al., 2009, Khan et al., 2008, Shah et al., 2008, Yun et al., 2008, Challa et al., 2007, Tomancak et al., 2007, Giot et al., 2003)

DmHtrA2

(Igaki et al., 2007, Igaki et al., 2003.6.16)

Dmel_CG8464

(Rawlings and Barrett, 1996-)

HtrA2/Omi

(Biskup, 2006)

Omi

(Kutscher and Shaham, 2017, Spinazzi and De Strooper, 2016, Muñoz-Soriano and Paricio, 2011, Whitworth et al., 2008, Yun et al., 2008)

Omi/HtrA2

(Guo, 2012)

dOmi

(Chung et al., 2020, Qi et al., 2016, Abdelwahid et al., 2011, Xu et al., 2009, Khan et al., 2008, Challa et al., 2007)

domi

(Challa et al., 2007, Park et al., 2006)

htrA2

(Chan and McQuibban, 2013, Yacobi-Sharon et al., 2013, Whitworth et al., 2008)

htrA2/omi

(Yacobi-Sharon et al., 2013)

omi

(Napoletano et al., 2017, Chan and McQuibban, 2013, Yacobi-Sharon et al., 2013, Whitworth et al., 2008, Yun et al., 2008, Park et al., 2006)

Name Synonyms

HTRA2-related serine protease

(Steller et al., 2014.7.9)

HtrA2

Serine protease HTRA2

(Banerjee and Chinthapalli, 2014)

high temperature requirement A2

(Chan and McQuibban, 2013)

Secondary FlyBase IDs

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

References (66)

The following external sites may use different assemblies or annotations than FlyBase.

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