Gene: Dmel\spas
Dspastin, D-spastin
AAA (TPase ssociated with diverse cellular ctivities) family member - regulates microtubule stability - mutants show defects in synaptic growth and neurotransmission
Please see the JBrowse view of Dmel\spas for information on other features
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Gene model reviewed during 5.56
Gene model reviewed during 5.43
Gene model reviewed during 5.46
Gene model reviewed during 6.07
Homohexamer. The homohexamer is stabilized by ATP-binding. The homohexamer may adopt a ring conformation through which microtubules pass prior to being severed (PubMed:18202664). Interacts with microtubules (PubMed:15823537, PubMed:18202664). Interacts with atl; may be involved in microtubule dynamics (PubMed:19341724).
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\spas using the Feature Mapper tool.
High levels of maternally supplied spas transcript is ubiquitously distributed in embryos through embryonic stage 4. At cellularization, expression is strongest near the basal side of the forming cell layer. During germband extension, spas is expressed in the ventral head and trunk ectoderm, in cells near the cephalic furrow, and in the invaginating midgut and hindgut primordia. At embryonic stage 13, spas transcript is expressed cells of all CNS neuromeres, including the subesophageal and supraesophageal ganglia. At embryonic stages 16-17, expression is pronounced in cell clusters throughout the supraesophageal ganglion, in the subesophageal ganglion, in two broad longitudial stripes in the ventral nerve cord, as well as weaker expression in some midline cells of the ventral nerve cord. Expression is also observed in the peripheral sense organs of the head, including Bolwig's organ and the dorsal organs.
GBrowse - Visual display of RNA-Seq signals
View Dmel\spas in GBrowse 23-82
3-82.5
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Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
polyclonal
Source for identity of: spas CG5977
spas loss causes neuronal defects that cannot be overcome by bypassing normal nociceptive transduction mechanisms.
High levels of spas disrupt microtubule organization within dendritic arbors.
The microtubule interacting and ATPase domains are sufficient for the ATP hydrolysis-dependent microtubule disassembly activity of spas.
spas appears to have a role in destabilising the microtubule cytoskeleton in neurons.
Targeted removal of spas from neurons result in loss of synaptic area but, paradoxically, strengthened neurotransmission. Overexpression of spas in neurons reduced synaptic efficiency. Loss of spas function leads to a dramatic increase in stabilised microtubules, which, in the synaptic terminal, inappropriately ramify beyond their normal axonal compartments into the synaptic boutons. Overexpression of spas causes an erosion of the stabilised microtubule network.
