FB2022_02, released March 29, 2022

Gene: Dmel\Obp99b

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General Information
Symbol
Dmel\Obp99b
Species
D. melanogaster
Name
Odorant-binding protein 99b
Annotation Symbol
CG7592
Feature Type
protein_coding_gene
FlyBase ID
FBgn0039685
Gene Model Status
Current
Stock Availability
2 publicly available
Gene Summary
Present in the aqueous fluid surrounding olfactory sensory dendrites and are thought to aid in the capture and transport of hydrophobic odorants into and through this fluid. (UniProt, Q9VAI6)
Contribute a Gene Snapshot for this gene.
All Summaries
Also Known As

tsx, 99b, Obp99d, turn on sex-specificity

Key Links
Genomic Location
Cytogenetic map
99B8-99B8
Sequence location
Recombination map
3-99
RefSeq locus
NT_033777 REGION:29716254..29716896
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
GO Summary Ribbons
Gene Ontology (GO) Annotations (6 terms)
Molecular Function (1 term)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
enables odorant binding
inferred from sequence or structural similarity with FLYBASE:lush; FB:FBgn0020277
(Vieira and Rozas, 2011)
non-traceable author statement
(assigned by UniProt )
(Kim and Smith, 2001)
Biological Process (4 terms)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (4 terms)
CV Term
Evidence
References
involved_in olfactory behavior
inferred from sequence or structural similarity with UniProtKB:O02372
(assigned by UniProt )
(UniProt Curators, 2002-)
involved_in response to pheromone
inferred from sequence or structural similarity with UniProtKB:O02372
(assigned by UniProt )
(UniProt Curators, 2002-)
involved_in sensory perception of chemical stimulus
inferred from sequence or structural similarity with FLYBASE:lush; FB:FBgn0020277
(Vieira and Rozas, 2011)
involved_in sensory perception of smell
inferred from biological aspect of ancestor with PANTHER:PTN000220814
(assigned by GO_Central )
(Gaudet et al., 2011)
Cellular Component (1 term)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
located_in extracellular region
inferred from sequence model
(Vieira and Rozas, 2011)
inferred from sequence or structural similarity with UniProtKB:O02372
(assigned by UniProt )
(UniProt Curators, 2002-)
Gene Group (FlyBase)
Protein Family (UniProt)
Belongs to the PBP/GOBP family. (Q9VAI6)
Protein Signatures (InterPro)
Summaries
Gene Group (FlyBase)
ODORANT BINDING PROTEINS -
Odorant Binding Proteins (OBP) are small (10 to 30 kDa) proteins secreted by auxiliary cells surrounding the olfactory receptor neurons. OBPs are characterized by a specific protein domain that comprises six α-helices joined by three disulfide bonds. Although the full function of the OBPs is not well established, it is believed that they may act as molecular carriers that transport odorants and deliver them to the olfactory receptors located on the sensory neurons. (Adapted from FBrf0205523).
Protein Function (UniProtKB)
Present in the aqueous fluid surrounding olfactory sensory dendrites and are thought to aid in the capture and transport of hydrophobic odorants into and through this fluid.
(UniProt, Q9VAI6)
Gene Model and Products
Number of Transcripts
2
Number of Unique Polypeptides
1

Please see the JBrowse view of Dmel\Obp99b for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Structure New Section
Protein 3D structure   (Predicted by AlphaFold)   (AlphaFold entry Q9VAI6)

If you don't see the viewer to the right, refresh your browser.
Model Confidence:
  • Very high (pLDDT > 90)
  • Confident (90 > pLDDT > 70)
  • Low (70 > pLDDT > 50)
  • Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Comments on Gene Model

Gene model reviewed during 5.46

Sequence Ontology: Class of Gene
nuclear_gene
protein_coding_gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
Obp99b-RA
FBtr0085462
NM_143456
574
149
Obp99b-RB
FBtr0333198
NM_001276149
586
149
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
Obp99b-PA
FBpp0084828
17.2
149
6.59
NP_651713
AAF56921
Obp99b-PB
FBpp0305400
17.2
149
6.59
NP_001263078
AGB96458
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

149 aa isoforms: Obp99b-PA, Obp99b-PB
Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Nucleotide / Polypeptide Records
 
UniProt
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Obp99b using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 11 -- 12
amnioserosa
(Fisher et al., 2012)
embryonic stage 13 -- 16
yolk nucleus
(Fisher et al., 2012)
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Obp99b in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyExpress - Embryonic expression images (BDGP data)
  • Stages(s) 13-16
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 0 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 4 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of Obp99b
Transgenic constructs containing regulatory region of Obp99b
Aberrations (Deficiencies and Duplications) ( 0 )
Inferred from experimentation ( 0 )
Inferred from location ( 0 )
Alleles Representing Disease-Implicated Variants
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
viable, with Dcr-2UAS.cDa, Scer\GAL4elav.PLu
viable, with Scer\GAL4Mef2.PR
viable, with Scer\GAL4pnr-MD237
Other Phenotypes
Allele
abnormal mating | female, with Scer\GAL4Act5C.PI
Phenotype manifest in
Allele
Orthologs
Downloads
Download All DIOPT Orthologs
Human Orthologs (via DIOPT v8.0)
Homo sapiens (Human) (0)
No records found.
Model Organism Orthologs (via DIOPT v8.0)
Mus musculus (laboratory mouse) (0)
No records found.
Rattus norvegicus (Norway rat) (0)
No records found.
Xenopus tropicalis (Western clawed frog) (0)
No records found.
Danio rerio (Zebrafish) (0)
No records found.
Caenorhabditis elegans (Nematode, roundworm) (0)
No records found.
Arabidopsis thaliana (thale-cress) (0)
No records found.
Saccharomyces cerevisiae (Brewer's yeast) (0)
No records found.
Schizosaccharomyces pombe (Fission yeast) (0)
No records found.
Other Organism Orthologs (via OrthoDB)
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v8.0)
Drosophila melanogaster (Fruit fly) (29)
Gene Symbol
Score
Source
Compara
eggNOG
Homologene
Inparanoid
Isobase
OrthoDB
OrthoFinder
orthoMCL
Panther
RoundUp
Alignment
Obp44a
3 of 10
Obp83g
3 of 10
Obp99a
3 of 10
Obp8a
2 of 10
Obp19d
2 of 10
Obp28a
2 of 10
Obp83a
2 of 10
Obp83b
2 of 10
Obp99c
2 of 10
Obp19b
1 of 10
Obp19c
1 of 10
Obp47a
1 of 10
Obp51a
1 of 10
Obp56a
1 of 10
Obp56b
1 of 10
Obp56c
1 of 10
Obp56d
1 of 10
Obp56e
1 of 10
Obp56f
1 of 10
Obp56g
1 of 10
Obp56h
1 of 10
Obp56i
1 of 10
Obp57c
1 of 10
Obp57d
1 of 10
Obp57e
1 of 10
Obp69a
1 of 10
Obp83cd
1 of 10
Obp83ef
1 of 10
Obp99d
1 of 10
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Model Summary Ribbon
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 0 )
    Human Ortholog
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Interaction
    References
    Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    Functional Complementation Data
    Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
    Interactions
    Summary of Physical Interactions
    esyN Network Diagram
    Interactions Browser
    Summary of Genetic Interactions
    esyN Network Diagram
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    External Data
    Linkouts
    DroID - A comprehensive database of gene and protein interactions.
    Pathways
    Signaling Pathways (FlyBase)
    Metabolic Pathways
    External Data
    Linkouts
    Genomic Location and Detailed Mapping Data
    Chromosome (arm)
    3R
    Recombination map
    3-99
    Cytogenetic map
    99B8-99B8
    Sequence location
    FlyBase Computed Cytological Location
    Cytogenetic map
    Evidence for location
    99B8-99B8
    Limits computationally determined from genome sequence between P{PZ}l(3)0674306743 and P{PZ}ncd05884
    Experimentally Determined Cytological Location
    Cytogenetic map
    Notes
    References
    Experimentally Determined Recombination Data
    Location

    3-99

    (FlyBase, 2016)

    3-96.7

    (Comeron et al., 2012)
    Left of (cM)
    Right of (cM)
    Notes
    Stocks and Reagents
    Stocks (2)
    Genomic Clones (11)
     

    Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

    cDNA Clones (295)
     

    Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

    cDNA clones, fully sequenced
    BDGP DGC clones
    Other clones
    Drosophila Genomics Resource Center cDNA clones

    For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

    cDNA Clones, End Sequenced (ESTs)
    BDGP DGC clones
    Other clones
    RNAi and Array Information
    Linkouts
    DRSC - Results frm RNAi screens
    GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
    Antibody Information
    Laboratory Generated Antibodies
     
    Commercially Available Antibodies
     
    Other Information
    Relationship to Other Genes
    Source for database identify of

    Source for identity of: Obp99b tsx

    (Fujii and Amrein, 2002)
    Source for database merge of

    Source for merge of: Obp99b BcDNA:LP06572

    (FlyBase, 1996-)
    Additional comments

    FlyBase curator comment: The gene referred to as "Obp99d" in FBrf0151704 is not the same gene as the one referred to as "Obp99d" in FlyBase (FBgn0039684). The gene referred to as "Obp99d" in FBrf0151704 actually corresponds to FBgn0039685 which is referred to in FlyBase with the symbol "Obp99b" (and corresponds to release 3 annotation CG7592).

    (Hekmat-Scafe et al., 2002)

    FlyBase curator comment: The gene referred to as "Obp99d" in FBrf0151651 is not the same gene as the one referred to as "Obp99d" in FlyBase (FBgn0039684). The gene referred to as "Obp99d" in FBrf0151651 actually corresponds to FBgn0039685 which is referred to in FlyBase with the symbol "Obp99b" (and corresponds to release 3 annotation CG7592).

    (Hekmat-Scafe, 2002.3.5)

    Source for merge of Obp99b BcDNA:LP06572 was a shared cDNA ( date:030728 ).

    (FlyBase, 1996-)
    Other Comments

    Identified as a gene with significant level of mRNA cycling as assessed by expression analysis using high density oligonucleotide arrays with probe generated from adult heads harvested over six time points over the course of a day. Shows alteration in expression in a Clk mutant background.

    (McDonald and Rosbash, 2001)
    Origin and Etymology
    Discoverer
    Etymology
    Identification
    External Crossreferences and Linkouts ( 34 )
    Sequence Crossreferences
    NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
    GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
    GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
    RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
    UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.
    UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
    UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
    Other crossreferences
    AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.
    BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
    Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
    DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
    EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
    Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
    FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
    FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
    Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
    Flygut - An atlas of the Drosophila adult midgut
    GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
    InterPro - A database of protein families, domains and functional sites
    KEGG Genes - Molecular building blocks of life in the genomic space.
    MARRVEL_MODEL - MARRVEL (model organism gene)
    modMine - A data warehouse for the modENCODE project
    Linkouts
    DroID - A comprehensive database of gene and protein interactions.
    DRSC - Results frm RNAi screens
    FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
    FlyMine - An integrated database for Drosophila genomics
    Synonyms and Secondary IDs (15)
    Reported As
    Symbol Synonym
    99b
    (Graham and Davies, 2002, Smith, 2002.1.14, Galindo and Smith, 2001)
    BcDNA:LP06572
    CG7592
    (Yadav et al., 2017, Çiçek et al., 2016, Palu and Thummel, 2016, Hosono et al., 2015, Vartiainen et al., 2014, Schertel et al., 2013, Fujikawa et al., 2009, Keleman et al., 2009.8.5, Liu et al., 2009, Reumer et al., 2009, Lai et al., 2007, Gorski et al., 2003, Vierstraete et al., 2003, Fujii and Amrein, 2002, Graham, 2002.6.18, Hekmat-Scafe, 2002.3.5, Hekmat-Scafe et al., 2002, Vogt et al., 2002, McDonald and Rosbash, 2001)
    DmelObp99b
    (Vieira and Rozas, 2011)
    OBP99b
    (Reumer et al., 2009)
    Obp99b
    (Pang et al., 2021, Rihani et al., 2021, Wang et al., 2021, Stoffel et al., 2020, He et al., 2019, Crocker et al., 2016, Kučerová et al., 2016, Moskalev et al., 2015, Paparazzo et al., 2015, Ratliff et al., 2015, Spratford and Kumar, 2015, Alic et al., 2014, Lai et al., 2014, Vartiainen et al., 2014, Handke et al., 2013, Kemp et al., 2013, Librado and Rozas, 2013, Martin et al., 2013, Schertel et al., 2013, Short and Lazzaro, 2013, Wang et al., 2013, Farhadian et al., 2012, Cherbas et al., 2011, Anholt and Williams, 2010, Buffin and Gho, 2010, Dalton et al., 2010, Fernández-Ayala et al., 2010, Wang et al., 2010, Dworkin et al., 2009, Fujikawa et al., 2009, Graze et al., 2009, Zhou et al., 2009, Allen and Spradling, 2008, Xun et al., 2008, Carney, 2007, Vieira et al., 2007, Wang et al., 2007, Wang et al., 2007)
    Obp99d
    (Hekmat-Scafe, 2002.3.5, Hekmat-Scafe et al., 2002)
    obp99b
    (Fan et al., 2020, Yamamoto et al., 2013)
    tsx
    (Fernández-Ayala et al., 2010, Goldman and Arbeitman, 2007, Wolfner, 2003, Anonymous, 2002, Fujii and Amrein, 2002)
    Name Synonyms
    General odorant-binding protein 99b precursor
    (Reumer et al., 2009)
    Odorant Binding Protein 99b
    (Lorbeck et al., 2011)
    Odorant binding protein 99B
    (Mosqueira et al., 2010)
    Odorant-binding protein 99b
    (Gao et al., 2021, Lai et al., 2014, Vartiainen et al., 2014, Zhang et al., 2011, Buffin and Gho, 2010, Carney, 2007, Galindo and Smith, 2001)
    Odordant-binding protein 99b
    (Liu et al., 2009)
    turn on sex-specificity
    (Goldman and Arbeitman, 2007, Anonymous, 2002)
    Secondary FlyBase IDs
    • FBgn0063111
    • FBgn0064137
    Datasets (8)
    Study focus (0)
    Experimental Role
    Project
    Project Type
    Title
    Study result (8)
    Result
    Result Type
    Title
    scRNAseq_2020_Cattenoz_NI_seq_clustering
    Clustering analysis of hemocytes from non-infested third instar larvae
    scRNAseq_2020_Cattenoz_WI_seq_clustering
    Clustering analysis of hemocytes from wasp-infested third instar larvae
    scRNAseq_2020_Cho_NI72LG_seq_clustering
    Clustering analysis of lymph gland cells from non-infested larvae at 72 h after egg-laying
    scRNAseq_2020_Cho_NI96LG_seq_clustering
    Clustering analysis of lymph gland cells from non-infested larvae at 96 h after egg-laying
    scRNAseq_2020_Cho_NI120LG_seq_clustering
    Clustering analysis of lymph gland cells from non-infested larvae at 120 h after egg-laying
    scRNAseq_2020_Cho_WI96LG_seq_clustering
    Clustering analysis of lymph gland cells from wasp-infested larvae at 96 h after egg-laying
    scRNAseq_2020_Cho_NI96HL_seq_clustering
    Clustering analysis of circulating hemocytes from non-infested larvae at 96 h after egg-laying
    scRNAseq_2020_Cho_NI120HL_seq_clustering
    Clustering analysis of circulating hemocytes from non-infested larvae at 120 h after egg-laying
    References (94)