FlyBase Gene Report: Dmel\pins

General Information

Symbol

Dmel\pins

Species

D. melanogaster

Name

partner of inscuteable

Annotation Symbol

CG5692

Feature Type

protein_coding_gene

FlyBase ID

FBgn0040080

Gene Model Status

Stock Availability

13 publicly available

Gene Snapshot

partner of inscuteable (pins) encodes a GDP-dissociation inhibitor involved in asymmetric cell division and mitotic spindle orientation. [Date last reviewed: 2019-06-13]

Other Summaries

Alliance
Auto summary
Interactive Fly

Also Known As

raps, Rapsynoid, Rad

Key Links

Genomic Location

Cytogenetic map

Sequence location

3R:27,582,840..27,586,376 [+]

Recombination map

3-94

RefSeq locus

NT_033777 REGION:27582840..27586376

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse GBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

Gene Ontology (GO) Annotations (12 terms)

Molecular Function (3 terms)

Terms Based on Experimental Evidence (3 terms)

CV Term

Evidence

References

enables GDP-dissociation inhibitor activity

inferred from direct assay

(assigned by UniProt )

(Yu et al., 2005)

enables protein binding

inferred from physical interaction with FLYBASE:Gαi; FB:FBgn0001104

(Nipper et al., 2007)

inferred from physical interaction with FLYBASE:mud; FB:FBgn0002873

(Nipper et al., 2007)

inferred from physical interaction with UniProtKB:P20353

(assigned by UniProt )

(Yu et al., 2005)

enables protein domain specific binding

inferred from physical interaction with FLYBASE:cno; FB:FBgn0259212

(Wee et al., 2011)

Terms Based on Predictions or Assertions (0 terms)

Biological Process (7 terms)

Terms Based on Experimental Evidence (5 terms)

CV Term

Evidence

References

involved_in asymmetric cell division

inferred from mutant phenotype

(Yu et al., 2003, Parmentier et al., 2000)

inferred from direct assay

(Januschke and Gonzalez, 2010)

involved_in asymmetric neuroblast division

inferred from mutant phenotype

(Bonaccorsi et al., 2007, Cai et al., 2003)

involved_in establishment of mitotic spindle orientation

inferred from mutant phenotype

(Ségalen et al., 2010, Yu et al., 2003)

inferred from direct assay

(Wee et al., 2011)

involved_in establishment of spindle orientation

inferred from mutant phenotype

(Siegrist and Doe, 2005)

involved_in neuroblast proliferation

inferred from mutant phenotype

(Caussinus and Gonzalez, 2005)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

involved_in asymmetric protein localization involved in cell fate determination

traceable author statement

(Gonczy, 2002)

involved_in basal protein localization

traceable author statement

(Peng et al., 2000)

Cellular Component (2 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

located_in apical cortex

inferred from direct assay

(Tsankova et al., 2017, Nipper et al., 2007, Siegrist and Doe, 2005, Yu et al., 2005)

located_in cell cortex

inferred from direct assay

(Wee et al., 2011)

Terms Based on Predictions or Assertions (0 terms)

Gene Group (FlyBase)

Protein Family (UniProt)

-

Protein Signatures (InterPro)

Summaries

Summary (Interactive Fly)

involved in asymmetric cell divisions and establishment of cell polarity - Prefoldin acts synergistically with Pins to regulate asymmetric division of both neuroblasts and intermediate neural progenitors

Interactive Fly

Gene Model and Products

Number of Transcripts

1

Number of Unique Polypeptides

1

Please see the JBrowse view of Dmel\pins for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Comments on Gene Model

Gene model reviewed during 5.46

Sequence Ontology: Class of Gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

FBtr0085189

3279

658

Additional Transcript Data and Comments

Reported size (kB)

3.3 (northern blot)

(Yu et al., 2000)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

FBpp0084559

71.5

658

6.73

Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments

Reported size (kDa)

658 (aa); 72 (kD predicted)

(Yu et al., 2000)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\pins using the Feature Mapper tool.

External Data

Crossreferences

Linkouts

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 5

epithelial cell

• centrosome & spindle microtubule

(Lecuyer et al., 2007)

• centrosome & spindle microtubule

(Lecuyer et al., 2007)

embryonic stage 1 -- 3

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

(Fisher et al., 2012)

embryonic stage 7 -- 8

organism | faint | ubiquitous

(Fisher et al., 2012)

embryonic stage 9 -- 10

organism | faint | ubiquitous

(Fisher et al., 2012)

embryonic stage 11 -- 12

anterior midgut primordium

(Fisher et al., 2012)

organism | faint | ubiquitous

(Fisher et al., 2012)

posterior midgut primordium

(Fisher et al., 2012)

embryonic stage 13 -- 16

embryonic/larval midgut

(Fisher et al., 2012)

embryonic/larval muscle system

(Fisher et al., 2012)

organism | faint | ubiquitous

(Fisher et al., 2012)

wandering third instar larval stage

wing pouch | restricted

(Molnar et al., 2012)

wing hinge primordium | restricted

(Molnar et al., 2012)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

mitotic domain 9

(Yu et al., 2000)

(Yu et al., 2000)

neuroblast MP2 | apical

• apical cortex

(Rath et al., 2002)

type I neuroblast | apical

(Chai et al., 2013)

Additional Descriptive Data

raps is observed as a crescent in the apical cortex in neuroblasts after delamination. It is also observed in cortical crescents in the dividing cells of procephalic mitotic domain 9.

(Yu et al., 2000)

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in apical cortex

inferred from direct assay

(Tsankova et al., 2017, Nipper et al., 2007, Siegrist and Doe, 2005, Yu et al., 2005)

located_in cell cortex

inferred from direct assay

(Wee et al., 2011)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\pins in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\pins

View exonic expression by tissue for Dmel\pins

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0040080

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0040080

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG5692-RA;CG3949-RA

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG5692

Flygut - An atlas of the Drosophila adult midgut

FBgn0040080

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\pins

Stages(s) 1-3
Stages(s) 4-6
Stages(s) 9-10
Stages(s) 11-12
Stages(s) 13-16

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 16 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 35 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of pins

Transgenic constructs containing regulatory region of pins

Aberrations (Deficiencies and Duplications) ( 5 )

Inferred from experimentation

Gene disrupted in

(Christensen et al., 2008.4.15)

(Christensen et al., 2008.4.15)

(Cook et al., 2008.12.28)

(Cook et al., 2009.2.28)

(Parmentier et al., 2000)

Inferred from location

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal - all die before end of pupal stage | recessive

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

pins^GD11737

viable, with Scer\GAL4^Mef2.PR

pins^GD11737

viable, with Scer\GAL4^pnr-MD237

pins^GD11737

viable, with Scer\GAL4^tin.CΔ4

pins^GD11737

Other Phenotypes

Allele

abnormal cell cycle

pins^P62

abnormal cell cycle (with pins^P62)

pins^P89

abnormal cell cycle (with pins^P89)

pins^P62

abnormal cell polarity

abnormal cell polarity (with pins^P62)

pins^P89

abnormal cell polarity (with pins^P89)

pins^P62

abnormal mitotic cell cycle

abnormal mitotic cell cycle (with pins¹⁹³)

pins^Δ50

abnormal mitotic cell cycle (with pins^Δ50)

pins¹⁹³

abnormal mitotic cell cycle | embryonic stage

pins^P89

abnormal mitotic cell cycle | P-stage

pins^P62

abnormal neuroanatomy

pins^P62

abnormal neuroanatomy, with Scer\GAL4^elav-C155

pins^EP3113

abnormal neuroanatomy | embryonic stage

pins^P89

abnormal neuroanatomy | larval stage, with Scer\GAL4^elav-C155

pins^EP3113

abnormal neuroanatomy | third instar larval stage

pins^P89

decreased cell number | larval stage

pins^P62

decreased cell number | larval stage | somatic clone

pins^P62

decreased cell number | third instar larval stage

pins^P89

some die during pupal stage | recessive

visible, with Scer\GAL4^pnr-MD237

pins^GD11737

Phenotype manifest in

Allele

anterior fascicle & synapse, with Scer\GAL4^elav-C155

pins^EP3113

anterior fascicle & synapse | supernumerary, with Scer\GAL4^elav-C155

pins^EP3113

pins^P62

centrosome (with pins^P62)

pins^P89

centrosome (with pins^P89)

pins^P62

embryonic neuroblast

pins^P62

embryonic neuroblast & spindle

ganglion mother cell GMC4-2a

pins^P89

intersegmental nerve, with Scer\GAL4^elav-C155

pins^EP3113

larval brain & neuroblast

pins^P62

larval neuroblast

pins^P62

mitotic cell cycle & spindle

mitotic domain 9 & epithelial cell

pins^P62

mitotic domain 9 & spindle

pins^P89

neuroblast | larval stage

pins¹⁹³

neuroblast | P-stage

pins^unspecified

neuroblast | third instar larval stage

pins^P89

RP2 motor neuron

RP2 motor neuron | ectopic

secondary neuroblast | third instar larval stage

pins^P89

sensory mother cell | P-stage

pins^P62

spindle & external sensory organ precursor cell I

pins^P62

spindle & larval neuroblast

pins^P62

spindle | P-stage

pins^P62

tormogen cell, with Scer\GAL4^pnr-MD237

pins^GD11737

pins^Δ50

wing margin (with pins¹⁹³)

pins^Δ50

wing margin (with pins^Δ50)

pins¹⁹³

Orthologs

Downloads

Download All DIOPT Orthologs

Download All OrthoDB Orthologs

Human Orthologs (via DIOPT v8.0)

Homo sapiens (Human) (4)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

13 of 15

Yes

Yes

12 of 15

No

Yes

2 of 15

No

No

1 of 15

No

Yes

Model Organism Orthologs (via DIOPT v8.0)

Mus musculus (laboratory mouse) (5)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

13 of 15

Yes

Yes

3

12 of 15

No

Yes

1 of 15

No

Yes

1 of 15

No

Yes

1 of 15

No

No

Rattus norvegicus (Norway rat) (5)

10 of 13

Yes

Yes

9 of 13

No

Yes

2 of 13

No

No

1 of 13

No

Yes

1 of 13

No

No

Xenopus tropicalis (Western clawed frog) (4)

8 of 12

Yes

Yes

7 of 12

No

Yes

2 of 12

No

No

Xtro\LOC108645506

1 of 12

No

Yes

Danio rerio (Zebrafish) (7)

12 of 15

Yes

Yes

8 of 15

No

Yes

7 of 15

No

Yes

2 of 15

No

Yes

1 of 15

No

Yes

1 of 15

No

No

1 of 15

No

No

Caenorhabditis elegans (Nematode, roundworm) (2)

13 of 15

Yes

Yes

1 of 15

No

No

Arabidopsis thaliana (thale-cress) (1)

1 of 9

Yes

Yes

Saccharomyces cerevisiae (Brewer's yeast) (0)

No records found.

Schizosaccharomyces pombe (Fission yeast) (0)

No records found.

Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG091904T9 )

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila suzukii

Spotted wing Drosophila

Dsuz\DS10_00009919

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Drosophila persimilis

Drosophila willistoni

Drosophila virilis

Drosophila mojavensis

Drosophila grimshawi

Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG0915012P )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Musca domestica

House fly

Glossina morsitans

Tsetse fly

Gmor\GMOY004448

Lucilia cuprina

Australian sheep blowfly

Lcup\FF38_11626

Mayetiola destructor

Hessian fly

Mdes\Mdes000288

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL008688

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL014195

Anopheles darlingi

American malaria mosquito

Adar\ADAC003392

Anopheles gambiae

Malaria mosquito

Agam\AGAP008977

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ010095

Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W00VJ )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Bombyx mori

Silkmoth

Bmor\BGIBMGA003867

Danaus plexippus

Monarch butterfly

Dple\DPOGS203008-PA

Heliconius melpomene

Postman butterfly

Hmel\HMEL011024

Apis florea

Little honeybee

Aflo\2563883492

Apis mellifera

Western honey bee

Bombus impatiens

Common eastern bumble bee

Bombus terrestris

Buff-tailed bumblebee

Linepithema humile

Argentine ant

Megachile rotundata

Alfalfa leafcutting bee

Mrot\MROTU:002878

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG005928

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG005926

Dendroctonus ponderosae

Mountain pine beetle

Tribolium castaneum

Red flour beetle

Pediculus humanus

Human body louse

Phum\PHUM426090

Rhodnius prolixus

Kissing bug

Rpro\RPRC007912

Cimex lectularius

Bed bug

Clec\CLEC000320

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI005773

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_06059

Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X05CB )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strigamia maritima

European centipede

Smar\SMAR000357

Stegodyphus mimosarum

African social velvet spider

Smim\KFM59201.1

Tetranychus urticae

Two-spotted spider mite

Turt\tetur09g05650

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_22062

Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G04C9 )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strongylocentrotus purpuratus

Purple sea urchin

Strongylocentrotus purpuratus

Purple sea urchin

Spur\SPU_008293gn

Strongylocentrotus purpuratus

Purple sea urchin

Strongylocentrotus purpuratus

Purple sea urchin

Spur\SPU_020502gn

Ciona intestinalis

Vase tunicate

Cint\ENSCING00000022449

Ciona intestinalis

Vase tunicate

Cint\ENSCING00000009076

Gallus gallus

Domestic chicken

Gallus gallus

Domestic chicken

Gallus gallus

Domestic chicken

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v8.0)

Drosophila melanogaster (Fruit fly) (2)

Gene Symbol

Score

Source

Alignment

2 of 10

1 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

cancer, neural stem cell, disruption of asymmetric division

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 2 )

Allele

Disease

Evidence

References

model of brain cancer

CEA with pins^P89

(Caussinus and Gonzalez, 2005)

model of cancer

CEA with pins^P89

(Janic et al., 2010)

model of brain cancer

CEA with pins^P62

(Caussinus and Gonzalez, 2005)

model of cancer

CEA with pins^P62

(Janic et al., 2010)

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

GPSM2; G protein signaling modulator 2

13 of 15

CHUDLEY-MCCULLOUGH SYNDROME; CMCS

GPSM1; G protein signaling modulator 1

12 of 15

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Dmel gene

Ortholog showing functional complementation

Supporting References

(Yu et al., 2003)

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

View in Interactions Browser

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

anti tag coimmunoprecipitation, western blot, anti tag western blot, pull down, anti bait coimmunoprecipitation, two hybrid, autoradiography

(Yoshiura et al., 2012, Kopein and Katanaev, 2009, Bowman et al., 2006, Hampoelz et al., 2005, Wang et al., 2005, Yu et al., 2005, Schaefer et al., 2001, Parmentier et al., 2000, Schaefer et al., 2000)

experimental knowledge based, pull down, western blot, anti bait coimmunoprecipitation, two hybrid, fluorescent resonance energy transfer, anti tag coimmunoprecipitation, autoradiography

(Lüchtenborg et al., 2015, Yoshiura et al., 2012, Guruharsha et al., 2011, Kopein and Katanaev, 2009, Katanaev and Tomlinson, 2006, Schaefer et al., 2000)

anti bait coimmunoprecipitation, western blot

(Blumer et al., 2003)

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

pins - RhoGAP18B

experimental knowledge based

(Guruharsha et al., 2011)

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

enzymatic study, autoradiography

(Johnston et al., 2009)

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

anti bait coimmunoprecipitation, western blot, pull down

(Wee et al., 2011, Speicher et al., 2008)

pull down, molecular weight estimation by staining, two hybrid, anti bait coimmunoprecipitation, western blot, anti tag coimmunoprecipitation, anti tag western blot

(Mauser and Prehoda, 2012, Siegrist and Doe, 2005, Bellaiche et al., 2001)

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

anti bait coimmunoprecipitation, western blot, two hybrid, pull down, molecular weight estimation by staining, autoradiography, anti tag coimmunoprecipitation, peptide massfingerprinting, static light scattering, molecular weight, x-ray crystallography, molecular sieving

(Culurgioni et al., 2018, Januschke et al., 2013, Mauser and Prehoda, 2012, Culurgioni et al., 2011, Schaefer et al., 2000, Yu et al., 2000)

anti bait coimmunoprecipitation, western blot, pull down

(Wang et al., 2005)

Summary of Genetic Interactions

esyN Network Diagram

esyN Network Key:

Suppression

Enhancement

View in Interactions Browser

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

enhanceable

(Katanaev and Tomlinson, 2006)

enhanceable

(Cai et al., 2003)

enhanceable

(Banerjee et al., 2017)

suppressible

(Zhang et al., 2016)

suppressible

(Zhang et al., 2016)

suppressible

(Andersen et al., 2012)

enhanceable

(Andersen et al., 2012)

suppressible

(Zhang et al., 2016)

suppressible

(Zhang et al., 2016)

suppressible

(Zhang et al., 2016)

suppressible

(Zhang et al., 2016)

suppressible

(Zhang et al., 2016)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

suppressible

(Guilgur et al., 2012)

suppressible

(Cai et al., 2003, Yu et al., 2003)

enhanceable

(Katanaev and Tomlinson, 2006)

enhanceable

(Yu et al., 2005)

enhanceable

(Banerjee et al., 2017)

enhanceable

(Zhang et al., 2016)

enhanceable

(Zhang et al., 2016)

enhanceable

(Andersen et al., 2012)

suppressible

(Andersen et al., 2012)

enhanceable

(Zhang et al., 2016)

enhanceable

(Zhang et al., 2016)

enhanceable

(Zhang et al., 2016)

enhanceable

(Zhang et al., 2016)

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

72799

DroID - A comprehensive database of gene and protein interactions.

FBgn0040080

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

53569

MIST (genetic) - An integrated Molecular Interaction Database

pins

MIST (protein-protein) - An integrated Molecular Interaction Database

pins

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

External Data

Linkouts

Genomic Location and Detailed Mapping Data

Chromosome (arm)

3R

Recombination map

3-94

Cytogenetic map

Sequence location

3R:27,582,840..27,586,376 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

98A8-98A8

Limits computationally determined from genome sequence between P{PZ}sda⁰³⁸⁸⁴ and P{PZ}btz^rL203

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

98A-98B

(Parmentier, 2000.2.15, Schaefer, 2000.3.6, Yu, 2000.1.31)

Experimentally Determined Recombination Data

Location

3-94

(FlyBase, 2016)

3-91.1

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (13)

32607

w¹¹¹⁸; P{EP}pins^G6902

29310

y¹ v¹; P{TRiP.JF02450}attP2

53968

y¹ v¹; P{TRiP.HMJ21351}attP40

6491

w^*; pins¹⁹³/TM6B, Tb¹

31808

w¹¹¹⁸; P{EP}pins^G16080/TM6C, Sb¹

6497

w^*; P{hs-pins.P}I1; pins⁸³/TM6B, Tb¹

77190

y¹ sc^* v¹ sev²¹; P{TKO.GS00670}attP40

79682

y¹ sc^* v¹ sev²¹; P{TOE.GS01341}attP40

35048

y¹ sc^* v¹ sev²¹; P{TRiP.HMS01462}attP2

37479

y¹ sc^* v¹ sev²¹; P{TRiP.GL00622}attP40

v330242

P{VSH330242}attP40

v27486

w¹¹¹⁸; P{GD11737}v27486/TM3

More stocks available...

Genomic Clones (20)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (126)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0040080

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0040080

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

53569

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Siegrist and Doe, 2005, Yu et al., 2002, Schaefer et al., 2001, Parmentier et al., 2000, Schaefer et al., 2000, Yu et al., 2000)

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for identity of: pins raps

(FlyBase, 2009-)

Source for database merge of

Source for merge of: Rad pins

(Parmentier et al., 2000)

Source for merge of: raps CG5692

(Bayraktaroglu, 2000.8.7)

Additional comments

'raps' renamed to 'pins' based on clear preference for the latter over the former in the published literature, and at the request of author O'Kane who first characterized and named the gene in FBrf0128604.

(FlyBase, 2009-)

Other Comments

baz, raps and dlg1 establish and maintain planar polarity in pI cells.

(Bellaiche et al., 2001)

raps is involved in the control of asymmetric divisions of neuroblasts.

(Parmentier et al., 2000)

In raps mutants, the asymmetric divisions of neuroblasts are altered; insc protein is no longer asymmetrically localized in the dividing neuroblast and the neuroblast division produces two daughter cells of similar sizes. However, the morphologically symmetrical divisions of raps mutant neuroblasts still lead to daughter cells with different fates, as shown by differences in gene expression.

(Parmentier et al., 2000)

raps and insc might orient asymmetric cell divisions by localising or locally modulating a heterotrimeric G-protein signalling cascade at the apical cell cortex.

(Schaefer et al., 2000)

Identification: identified as a protein that binds to insc in a yeast two-hybrid screen.

(Yu et al., 2000)

raps is required for all aspects of insc function.

(Yu et al., 2000)

raps is required for insc protein to asymmetrically localise.

(Yu et al., 2000)

Isolated using a yeast two-hybrid system.

(Woods et al., 1997)

raps encodes a putative SH3 binding protein, that co-localises with dlg1 at septate junctions.

(Woods et al., 1997)

Origin and Etymology

Discoverer

Etymology

The raps protein contains 7 tetratricopeptide repeats which are similar to those existing in "rapsyn", a mouse protein, hence the name "rapsynoid". However, raps is not a homologue of rapsyn.

(Parmentier et al., 2000)

Identification

External Crossreferences and Linkouts ( 46 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

53569

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0040080

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0040080

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG5692

Flygut - An atlas of the Drosophila adult midgut

FBgn0040080

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

53569

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC003257

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG5692

modMine - A data warehouse for the modENCODE project

FBgn0040080

Linkouts

BioGRID - A database of protein and genetic interactions.

72799

DroID - A comprehensive database of gene and protein interactions.

FBgn0040080

DRSC - Results frm RNAi screens

FBgn0040080

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0040080

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG5692-RA;CG3949-RA

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0040080

FlyMine - An integrated database for Drosophila genomics

FBgn0040080

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

53569

MIST (genetic) - An integrated Molecular Interaction Database

pins

MIST (protein-protein) - An integrated Molecular Interaction Database

pins

Synonyms and Secondary IDs (19)

Reported As

Symbol Synonym

CG5692

(Meadows, 2017.1.11, Neuert et al., 2017, Ni et al., 2011.7.19, Ni et al., 2010.12.1, Perkins et al., 2009, Molnar et al., 2006, Kraut et al., 2001, Kraut et al., 2001)

PINS

(Osses and Henríquez, 2014, Gonzalez, 2013, Saini and Reichert, 2012, Kurusu et al., 2009, Morrison and Kimble, 2006, Baas et al., 2004)

Pins

(Aguilar-Aragon et al., 2020, Majumdar and Liu, 2020, Magnaghi-Jaulin et al., 2019, Carmena, 2018, Dewey and Johnston, 2017, Lang and Munro, 2017, Richardson and Portela, 2017, Ajduk and Zernicka-Goetz, 2016, Moreira and Morais-de-Sá, 2016, Yasugi and Nishimura, 2016, Zhang et al., 2016, Bajaj et al., 2015, Bell et al., 2015, Hanlon and Andrew, 2015, Mauri et al., 2014, Tipping and Perrimon, 2014, Bergstralh et al., 2013, Chai et al., 2013, Culurgioni and Mapelli, 2013, Lu and Johnston, 2013, Lu and Prehoda, 2013, Andersen et al., 2012, Guilgur et al., 2012, Homem and Knoblich, 2012, Kelsom and Lu, 2012, Smith and Prehoda, 2011, Wee et al., 2011, Cabernard et al., 2010, Januschke and Gonzalez, 2010, Cabernard and Doe, 2009, Gomes et al., 2009, Johnston et al., 2009, Kopein and Katanaev, 2009, Mummery-Widmer et al., 2009, Sahota et al., 2009, Boone and Doe, 2008, Chia et al., 2008, Krahn and Wodarz, 2008, Lin, 2008, Speicher et al., 2008, Yamashita and Fuller, 2008, Slack et al., 2007, Smith et al., 2007, Sommer et al., 2007, Bowman et al., 2006, Hutterer et al., 2006, Katanaev and Tomlinson, 2006, Lee et al., 2006, Lee et al., 2006, Ahringer, 2005, Budde, 2005, David et al., 2005, Gomes and Schweisguth, 2005, Hampoelz et al., 2005, Izumi et al., 2005, Jafar-Nejad et al., 2005, Siegrist and Doe, 2005, Wang et al., 2005, Yamashita et al., 2005, Yu et al., 2005, Bardin et al., 2004, Bellaiche et al., 2004, Betschinger and Knoblich, 2004, Dunlop et al., 2004, Hampoelz and Knoblich, 2004, Izumi et al., 2004, Roegiers and Jan, 2004, Siegrist and Doe, 2004, Torban et al., 2004, Blumer et al., 2003, Hidalgo and ffrench-Constant, 2003, Izumi et al., 2003, Rolls et al., 2003, Rothenberg et al., 2003, Schweisguth et al., 2003, Wodarz and Huttner, 2003, Gonczy, 2002, Le Borgne et al., 2002, Bellaiche et al., 2001, Cai et al., 2001, Dawes-Hoang and Wieschaus, 2001, Doe, 2001, Knust, 2001, Ohno, 2001, Roegiers et al., 2001, Schaefer et al., 2001, Schaefer et al., 2001, Schaefer et al., 2000)

RAPS

(Gonzalez, 2013)

Rad

(Woods et al., 1997)

Raps

(Auld et al., 2018, Lu and Johnston, 2013, Mudiganti et al., 2010, Sahota et al., 2009, Parmentier et al., 2000)

dLGN

(Culurgioni et al., 2018)

dPins

(Saadaoui et al., 2017)

pins

(Ryder et al., 2020, Finegan et al., 2019, Franco and Carmena, 2019, Hu and Jasper, 2019, Pham et al., 2019, Saito et al., 2018, Banerjee et al., 2017, Roubinet et al., 2017, Transgenic RNAi Project members, 2017-, Tsankova et al., 2017, Bosveld et al., 2016, Cate et al., 2016, Zhang et al., 2016, Bell et al., 2015, Bergstralh et al., 2015, Besson et al., 2015, Keder et al., 2015, Schweisguth, 2015, Poulton et al., 2014, Bergstralh et al., 2013, Januschke et al., 2013, Jauffred et al., 2013, Andersen et al., 2012, Carney et al., 2012, Guilgur et al., 2012, Yoshiura et al., 2012, Furman and Bukharina, 2011, Miles et al., 2011, Wang et al., 2011, Chang et al., 2010, Janic et al., 2010, Januschke and Gonzalez, 2010, Ségalen et al., 2010, Bonaccorsi et al., 2007, Nipper et al., 2007, Rebollo et al., 2007, Tountas and Fortini, 2007, Izumi et al., 2006, Lee et al., 2006, Siller et al., 2006, Wang et al., 2006, Wang et al., 2006, Wodarz and Gonzalez, 2006, Budde, 2005, Caussinus and Gonzalez, 2005, David et al., 2005, Jafar-Nejad et al., 2005, Langevin et al., 2005, Yu et al., 2005, Lee et al., 2004, Ahringer, 2003, Albertson and Doe, 2003, Cai et al., 2003, Fuse et al., 2003, Le Borgne and Schweisguth, 2003, Radovic and Bryant, 2003, Tuxworth and Chia, 2003, Wallenfang and Matunis, 2003, Yu et al., 2003, Yu et al., 2003, Adler, 2002, Chia and Yang, 2002, Kaltschmidt and Brand, 2002, Rath et al., 2002, Yu et al., 2002, Bellaiche et al., 2001, Chase, 2001, Doe, 2001, Doe and Bowerman, 2001, Gulli and Peter, 2001, Kraut et al., 2001, Kraut et al., 2001, Parmentier et al., 2001, Schaefer and Knoblich, 2001, Schaefer et al., 2001, Tio et al., 2001, Wodarz, 2001, Brazil and Hemmings, 2000, Jan and Jan, 2000, Leevers, 2000, Ohshiro et al., 2000, Peng et al., 2000, Schaefer, 2000.3.6, Schaefer et al., 2000, Schweisguth, 2000, Yu, 2000.1.31, Yu et al., 2000, Yu et al., 2000)

pins/raps

(Azevedo and Baylies, 2020)

rad

(Radovic et al., 2000)

raps

(Neuert et al., 2017, Álvarez-Fernández et al., 2015, Schulman et al., 2014, Vaque et al., 2013, Friedman et al., 2011, Cook et al., 2009.2.28, Christensen et al., 2008.4.15, Christensen et al., 2008.4.15, Cook et al., 2008.12.28, Lecuyer et al., 2007, Zhang et al., 2007, Molnar et al., 2006, Caussinus and Gonzalez, 2005, Clevers, 2005)

rapsyn

(Littleton and Ganetzky, 2000)

Name Synonyms

Partner of Inscutable

(Tepass, 2002)

Partner of Inscuteable

(Magnaghi-Jaulin et al., 2019, Bergstralh et al., 2013, Gonzalez, 2013, Saini and Reichert, 2012, Sahota et al., 2009, Boone and Doe, 2008, Chia et al., 2008, Lin, 2008, Speicher et al., 2008, Nipper et al., 2007, David et al., 2005, Bardin et al., 2004, Lai and Orgogozo, 2004, Roegiers and Jan, 2004, Anonymous, 2003, Yu et al., 2002, Bellaiche et al., 2001)

Rapsinoid

(Kopein and Katanaev, 2009)

Rapsynoid

(Mummery-Widmer et al., 2009, Sahota et al., 2009, Kraut et al., 2001, Kraut et al., 2001, Woods et al., 1997)

partner of inscuteable

(Azevedo and Baylies, 2020, Furman and Bukharina, 2011, Miles et al., 2011, Janic et al., 2010, Tountas and Fortini, 2007, Wodarz and Gonzalez, 2006, Ahringer, 2003, Skeath and Thor, 2003, Brazil and Hemmings, 2000, Cheah et al., 2000, Yu, 2000.1.31, Yu et al., 2000)

rapsynoid

(Taniguchi et al., 2014, Cheah et al., 2000)

Secondary FlyBase IDs

FBgn0020384
FBgn0039526

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

References (260)