FlyBase Gene Report: Dmel\Nhe2

General Information

Symbol

Dmel\Nhe2

Species

D. melanogaster

Name

Na⁺/H⁺ hydrogen exchanger 2

Annotation Symbol

CG9256

Feature Type

protein_coding_gene

FlyBase ID

FBgn0040297

Gene Model Status

Current

Stock Availability

18 publicly available

Gene Summary

Na[+]/H[+] hydrogen exchanger 2 (Nhe2) encodes a Na-H exchanger of the SLC family 9A that increases intracellular pH. The product of Nhe2 activity promotes proliferation, morphogenesis and planar cell polarity, and contributes to oncogene-induced metastatic cell behaviors. [Date last reviewed: 2019-09-26] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
Interactive Fly

Also Known As

DmNHE2

Key Links

Genomic Location

Cytogenetic map

39A2-39A3

Sequence location

2L:21,104,568..21,134,255 [+]

Recombination map

2-54

RefSeq locus

NT_033779 REGION:21104568..21134255

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse GBrowse

Help me choose

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

Gene Ontology (GO) Annotations (13 terms)

Molecular Function (2 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

enables potassium:proton antiporter activity

inferred from biological aspect of ancestor with PANTHER:PTN000011605

(assigned by GO_Central )

(Gaudet et al., 2011)

enables sodium:proton antiporter activity

inferred from biological aspect of ancestor with PANTHER:PTN000011605

(assigned by GO_Central )

(Gaudet et al., 2011)

Biological Process (8 terms)

Terms Based on Experimental Evidence (5 terms)

CV Term

Evidence

References

involved_in cellular ion homeostasis

inferred from expression pattern

(Day et al., 2008)

involved_in compound eye morphogenesis

inferred from mutant phenotype

(Grillo-Hill et al., 2015)

involved_in intracellular pH elevation

inferred from mutant phenotype

(Ulmschneider et al., 2016)

involved_in regulation of cellular pH

inferred from mutant phenotype

(Grillo-Hill et al., 2015)

involved_in renal system process

inferred from genetic interaction with FLYBASE:CG10939; FB:FBgn0010620

(Ghimire et al., 2019)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

involved_in potassium ion transmembrane transport

inferred from biological aspect of ancestor with PANTHER:PTN000011605

(assigned by GO_Central )

(Gaudet et al., 2011)

involved_in regulation of intracellular pH

inferred from biological aspect of ancestor with PANTHER:PTN000011605

(assigned by GO_Central )

(Gaudet et al., 2011)

involved_in sodium ion import across plasma membrane

inferred from biological aspect of ancestor with PANTHER:PTN000011605

(assigned by GO_Central )

(Gaudet et al., 2011)

Cellular Component (3 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

located_in apical plasma membrane

inferred from direct assay

(Ghimire et al., 2019)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

located_in integral component of membrane

inferred from electronic annotation with InterPro:IPR004709, InterPro:IPR006153, InterPro:IPR018422

(assigned by InterPro )

(FlyBase Curators et al., 2004-)

located_in plasma membrane

inferred from biological aspect of ancestor with PANTHER:PTN000011605

(assigned by GO_Central )

(Gaudet et al., 2011)

Gene Group (FlyBase)

SLC9 FAMILY OF SODIUM/PROTON EXCHANGERS

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

Gene Group (FlyBase)

SLC9 FAMILY OF SODIUM/PROTON EXCHANGERS -: The solute carrier family 9 (SLC9) members are Na⁺/H⁺ exchangers that maintain cellular pH by utilising the sodium gradient across the plasma membrane to extrude protons produced by metabolism. (Adapted from FBrf0229646).

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Nhe2 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Comments on Gene Model

Gene model reviewed during 5.47

Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.

Low-frequency RNA-Seq exon junction(s) not annotated.

Annotated transcripts do not represent all supported alternative splices within 5' UTR.

Gene model reviewed during 5.53

Gene model reviewed during 6.13

Sequence Ontology: Class of Gene

gene_with_dicistronic_mRNA

(FlyBase Genome Annotators, 2016, Mackowiak et al., 2015)

gene_with_edited_transcript

(Rodriguez et al., 2012, Graveley et al., 2011)

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Nhe2-RA

FBtr0081466

NM_165355

4788

1291

Nhe2-RB

FBtr0081467

NM_136244

4794

1291

Nhe2-RC

FBtr0299908

NM_001144375

4578

1219

Nhe2-RD

FBtr0299909

NM_001144376

2838

672

Nhe2-RE

FBtr0299910

NM_001144377

4602

1229

Nhe2-RF

FBtr0299911

NM_001144378

2741

723

Nhe2-RG

FBtr0299912

NM_001144379

6233

1322

Nhe2-RH

FBtr0306015

NM_001259187

5898

1222

Nhe2-RI

FBtr0306016

NM_001259188

6466

1166

Nhe2-RJ

FBtr0334171

NM_001273731

4432

1305

Nhe2-RK

FBtr0334172

NM_001273732

4792

1177

Nhe2-RL

FBtr0334173

NM_001273733

4686

1257

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

Nhe2-PA

FBpp0080995

142.3

1291

6.84

NP_724311

AAF53960

Nhe2-PB

FBpp0080996

142.3

1291

6.84

NP_610088

AAF53961

Nhe2-PC

FBpp0289186

134.4

1219

7.10

NP_001137847

ACL83053

Nhe2-PD

FBpp0289187

74.4

672

8.79

NP_001137848

ACL83054

Nhe2-PE

FBpp0289188

135.6

1229

7.19

NP_001137849

ACL83055

Nhe2-PF

FBpp0289189

80.4

723

9.33

NP_001137850

ACL83056

Nhe2-PG

FBpp0289190

146.1

1322

7.46

NP_001137851

ACL83057

Nhe2-PH

FBpp0297157

134.6

1222

8.98

NP_001246116

AFH03790

Nhe2-PI

FBpp0297158

129.4

1166

9.06

NP_001246117

AFH03791

Nhe2-PJ

FBpp0306288

143.9

1305

6.97

NP_001260660

AGB93195

Nhe2-PK

FBpp0306289

129.7

1177

8.92

NP_001260661

AGB93196

Nhe2-PL

FBpp0306290

139.2

1257

7.43

NP_001260662

AGB93197

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

1291 aa isoforms: Nhe2-PA, Nhe2-PB

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Nhe2 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Linkouts

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 4 -- 6

organism | faint | ubiquitous

(Fisher et al., 2012)

embryonic stage 7 -- 8

head mesoderm

(Fisher et al., 2012)

trunk mesoderm

(Fisher et al., 2012)

embryonic stage 9 -- 10

antennal primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)

central brain primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)

visual primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)

dorsal head epidermis primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)

lateral head epidermis primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)

ventral head epidermis primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)

anterior endoderm

(Fisher et al., 2012)

head mesoderm

(Fisher et al., 2012)

posterior endoderm

(Fisher et al., 2012)

trunk mesoderm

(Fisher et al., 2012)

ventral nerve cord primordium

(Fisher et al., 2012)

microinjection

Stage

Tissue/Position (including subcellular localization)

Reference

larval stage

embryonic/larval midgut

Comment: enriched in acidic region

(Overend et al., 2016)

Additional Descriptive Data

RNA-seq data show that Nhe2 is expressed throughout the larval midgut but is enriched in the acidic region of the midgut.

(Overend et al., 2016)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

(Day et al., 2008)

(Day et al., 2008)

(Day et al., 2008)

adult Malpighian tubule

(Day et al., 2008)

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in apical plasma membrane

inferred from direct assay

(Ghimire et al., 2019)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Nhe2 in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Nhe2

View exonic expression by tissue for Dmel\Nhe2

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0040297

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0040297

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG9256-RA

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG9256

Flygut - An atlas of the Drosophila adult midgut

FBgn0040297

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\Nhe2

Stages(s) 4-6
Stages(s) 7-8
Stages(s) 9-10
Stages(s) 11-12
Stages(s) 13-16

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 26 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 11 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Nhe2

Transgenic constructs containing regulatory region of Nhe2

Aberrations (Deficiencies and Duplications) ( 2 )

Inferred from experimentation ( 2 )

Gene disrupted in

Df(2L)Exel6047

(Ryder, 2004.4.26)

Gene partially disrupted in

Df(2L)Nhe2^null

(Grillo-Hill et al., 2015)

Inferred from location ( 0 )

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

cell lethal | recessive | somatic clone

Nhe2¹

lethal - all die before end of larval stage | recessive

Nhe2¹

partially lethal - majority die

Nhe2^null

viable

Nhe2^EY11323

viable, with Scer\GAL4^Mef2.PR

Nhe2^GD3477

viable (with Df(2L)BSC302)

Nhe2^{MI03660-TG4.1}

Sterility

Allele

fertile

Nhe2^EY11323

Other Phenotypes

Allele

abnormal cell shape | oogenesis | oogenesis

Nhe2^null

abnormal planar polarity, with Nhe2¹, Scer\GAL4^hs.2sev

Nhe2^{dsRNA.UAS.WIZ}

abnormal planar polarity, with Nhe2^{dsRNA.UAS.WIZ}, Scer\GAL4^hs.2sev

Nhe2¹

abnormal planar polarity, with Scer\GAL4^hs.2sev

abnormal planar polarity (with Nhe2¹), with Scer\GAL4^hs.2sev

Nhe2^{dsRNA.UAS.WIZ}

abnormal planar polarity (with Nhe2^{dsRNA.UAS.WIZ}), with Scer\GAL4^hs.2sev

Nhe2¹

decreased cell number | oogenesis | oogenesis

Nhe2^null

decreased fecundity

Nhe2^null

increased cell number, with Scer\GAL4^en.PU

Nhe2^UAS.cGa

increased occurrence of cell division | larval stage, with Scer\GAL4^GMR.PU

Nhe2^UAS.cGa

increased occurrence of cell division | pupal stage, with Scer\GAL4^GMR.PU

Nhe2^UAS.cGa

some die during larval stage | recessive

Nhe2¹

visible, with Scer\GAL4^ey.3.5.Exel

Nhe2^UAS.cGa

visible, with Scer\GAL4^GMR.PU

Nhe2^{UAS.E358I.Tag:HA}

visible | adult stage, with Scer\GAL4^GMR.PU

Nhe2^UAS.cGa

Phenotype manifest in

Allele

basal stalk

Nhe2^null

basal stalk, with Scer\GAL4^109-30

cone cell | pupal stage, with Scer\GAL4^GMR.PU

Nhe2^UAS.cGa

embryonic/larval digestive system

Nhe2¹

embryonic/larval fat body

Nhe2¹

eye, with Nhe2¹, Scer\GAL4^hs.2sev

Nhe2^{dsRNA.UAS.WIZ}

eye, with Nhe2^{dsRNA.UAS.WIZ}, Scer\GAL4^hs.2sev

Nhe2¹

eye, with Scer\GAL4^ey.3.5.Exel

Nhe2^UAS.cGa

eye, with Scer\GAL4^GMR.PU

eye, with Scer\GAL4^hs.2sev

Nhe2^long.UAS

eye (with Nhe2¹), with Scer\GAL4^hs.2sev

Nhe2^{dsRNA.UAS.WIZ}

eye (with Nhe2^{dsRNA.UAS.WIZ}), with Scer\GAL4^hs.2sev

Nhe2¹

eye disc posterior to the morphogenetic furrow, with Scer\GAL4^GMR.PU

Nhe2^UAS.cGa

eye photoreceptor cell, with Scer\GAL4^GMR.PU

Nhe2^UAS.cGa

eye photoreceptor cell, with Scer\GAL4^hs.2sev

Nhe2^short.UAS

female germline cyst

Nhe2^null

female germline cyst, with Scer\GAL4^109-30

female germline stem cell | oogenesis | oogenesis

Nhe2^null

female germline stem cell | oogenesis | oogenesis, with Scer\GAL4^109-30

follicle cell

Nhe2^null

follicle cell, with Scer\GAL4^109-30

germarium

Nhe2^null

germarium, with Scer\GAL4^109-30

interommatidial bristle, with Scer\GAL4^GMR.PU

Nhe2^{UAS.E358I.Tag:HA}

ommatidium, with Nhe2¹, Scer\GAL4^hs.2sev

Nhe2^{dsRNA.UAS.WIZ}

ommatidium, with Nhe2^{dsRNA.UAS.WIZ}, Scer\GAL4^hs.2sev

Nhe2¹

ommatidium, with Scer\GAL4^GMR.PU

Nhe2^UAS.cGa

ommatidium, with Scer\GAL4^hs.2sev

Nhe2^short.UAS

ommatidium (with Nhe2¹), with Scer\GAL4^hs.2sev

Nhe2^{dsRNA.UAS.WIZ}

ommatidium (with Nhe2^{dsRNA.UAS.WIZ}), with Scer\GAL4^hs.2sev

Nhe2¹

ommatidium | pupal stage, with Scer\GAL4^GMR.PU

Nhe2^UAS.cGa

primary pigment cell, with Scer\GAL4^GMR.PU

Nhe2^UAS.cGa

retina

Nhe2^null

retina | pupal stage, with Scer\GAL4^GMR.PU

Nhe2^UAS.cGa

secondary pigment cell

Nhe2^null

stalk follicle cell

Nhe2^null

stalk follicle cell, with Scer\GAL4^109-30

wing disc posterior compartment, with Scer\GAL4^en.PU

Nhe2^UAS.cGa

Orthologs

Downloads

Download All DIOPT Orthologs

Download All OrthoDB Orthologs

Human Orthologs (via DIOPT v8.0)

Homo sapiens (Human) (13)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Hsap\SLC9A3

12 of 15

Yes

Hsap\SLC9A5

12 of 15

Yes

Hsap\SLC9A2

6 of 15

Yes

Hsap\SLC9A1

5 of 15

Yes

Hsap\SLC9A4

5 of 15

Yes

Hsap\SLC9A9

2 of 15

Hsap\SLC9C1

2 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Yes

Model Organism Orthologs (via DIOPT v8.0)

Mus musculus (laboratory mouse) (12)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Mmus\Slc9a5

12 of 15

Yes

Mmus\Slc9a3

10 of 15

Yes

Mmus\Slc9a2

6 of 15

Yes

Mmus\Slc9a1

5 of 15

Yes

Mmus\Slc9a4

5 of 15

Yes

Mmus\Slc9a9

2 of 15

Mmus\Slc9c1

2 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Rattus norvegicus (Norway rat) (12)

Rnor\Slc9a3

9 of 13

Yes

Rnor\Slc9a5

8 of 13

Yes

Rnor\Slc9a1

5 of 13

Yes

Rnor\Slc9a2

5 of 13

Yes

Rnor\Slc9a4

4 of 13

Yes

2 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Xenopus tropicalis (Western clawed frog) (11)

Xtro\slc9a5

7 of 12

Yes

Xtro\slc9a1

4 of 12

Yes

Xtro\LOC100488264

3 of 12

Yes

Xtro\slc9a3

2 of 12

Yes

Xtro\slc9a9

2 of 12

Xtro\slc9a2

1 of 12

Yes

Xtro\slc9a4

1 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

Danio rerio (Zebrafish) (10)

Drer\slc9a3.1

10 of 15

Yes

Drer\slc9a5

8 of 15

Yes

Drer\slc9a3.2

7 of 15

Yes

Drer\slc9a2

5 of 15

Yes

Drer\slc9a1b

2 of 15

Yes

Drer\si:dkey-162b23.4

1 of 15

Drer\slc9a1a

1 of 15

Yes

Drer\slc9a6a

1 of 15

Drer\slc9a6b

1 of 15

Drer\slc9a8

1 of 15

Caenorhabditis elegans (Nematode, roundworm) (11)

Cele\nhx-3

10 of 15

Yes

Cele\nhx-2

9 of 15

Yes

Cele\nhx-9

9 of 15

Yes

Cele\nhx-6

8 of 15

Yes

Cele\nhx-1

5 of 15

Yes

Cele\nhx-4

3 of 15

Yes

Cele\pbo-4

3 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

Arabidopsis thaliana (thale-cress) (6)

Atha\NHX1

1 of 9

Yes

Atha\NHX2

1 of 9

Yes

Atha\NHX3

1 of 9

Yes

Atha\NHX4

1 of 9

Yes

Atha\NHX5

1 of 9

Yes

Atha\NHX6

1 of 9

Yes

Saccharomyces cerevisiae (Brewer's yeast) (2)

Scer\NHX1

2 of 15

Yes

Scer\NHA1

1 of 15

Yes

Schizosaccharomyces pombe (Fission yeast) (1)

Spom\SPAC15A10.06

1 of 12

Yes

Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG0919011B )

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila suzukii

Spotted wing Drosophila

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA21648

Drosophila persimilis

Dper\GL25889

Drosophila willistoni

Dwil\GK24318

Drosophila virilis

Dvir\GJ16135

Drosophila mojavensis

Dmoj\GI21064

Drosophila grimshawi

Dgri\GH13669

Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091500LX )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Musca domestica

House fly

Mdoa\17027459

Musca domestica

House fly

Mdoa\17006283

Musca domestica

House fly

Mdoa\17006312

Glossina morsitans

Tsetse fly

Gmor\GMOY006250

Lucilia cuprina

Australian sheep blowfly

Lcup\FF38_07080

Mayetiola destructor

Hessian fly

Mdes\Mdes008546

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL001503

Anopheles darlingi

American malaria mosquito

Adar\ADAC004664

Anopheles gambiae

Malaria mosquito

Agam\AGAP009036

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ009357

Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W00WD )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Bombyx mori

Silkmoth

Bmor\BGIBMGA011831

Danaus plexippus

Monarch butterfly

Dple\DPOGS213550-PA

Heliconius melpomene

Postman butterfly

Hmel\HMEL003119

Apis florea

Little honeybee

Aflo\2611049652

Apis florea

Little honeybee

Aflo\2611049796

Apis mellifera

Western honey bee

Amel\GB42942

Bombus impatiens

Common eastern bumble bee

Bimp\BIMP10068

Bombus terrestris

Buff-tailed bumblebee

Bter\15213939

Linepithema humile

Argentine ant

Lhum\LH22274

Megachile rotundata

Alfalfa leafcutting bee

Mrot\MROTU:0002c5

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG021504

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG021178b

Dendroctonus ponderosae

Mountain pine beetle

Dpod\YQE_03811

Dendroctonus ponderosae

Mountain pine beetle

Dpod\YQE_03812

Tribolium castaneum

Red flour beetle

Tcas\TC031052

Pediculus humanus

Human body louse

Phum\PHUM467900

Rhodnius prolixus

Kissing bug

Rpro\RPRC004960

Cimex lectularius

Bed bug

Clec\CLEC008515

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_01169

Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X023O )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strigamia maritima

European centipede

Smar\SMAR014264

Ixodes scapularis

Black-legged tick

Isca\ISCW017041

Stegodyphus mimosarum

African social velvet spider

Smim\KFM62765.1

Stegodyphus mimosarum

African social velvet spider

Smim\KFM58751.1

Stegodyphus mimosarum

African social velvet spider

Smim\KFM62766.1

Tetranychus urticae

Two-spotted spider mite

Turt\tetur07g01290

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_217278

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_129880

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_301159

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_300379

Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G02Q0 )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Slc9a2

Ciona intestinalis

Vase tunicate

Cint\LOC100177620

Gallus gallus

Domestic chicken

Ggal\ENSGALG00000025791

Gallus gallus

Domestic chicken

Ggal\ENSGALG00000016791

Gallus gallus

Domestic chicken

Ggal\ENSGALG00000007867

Gallus gallus

Domestic chicken

Ggal\ENSGALG00000016790

Gallus gallus

Domestic chicken

Ggal\ENSGALG00000000921

Gallus gallus

Domestic chicken

Ggal\ENSGALG00000012517

Gallus gallus

Domestic chicken

Ggal\ENSGALG00000007118

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v8.0)

Drosophila melanogaster (Fruit fly) (4)

Gene Symbol

Score

Source

Alignment

3 of 10

3 of 10

1 of 10

1 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Allele

Disease

Evidence

References

Nhe2^KK102518

model of nephrolithiasis

CEA

(Ghimire et al., 2019)

Potential Models Based on Orthology ( 2 )

Human Ortholog

Disease

Evidence

References

SLC9A3; solute carrier family 9 member A3

model of congenital secretory sodium diarrhea 8

IEA

(FlyBase, 2019-)

SLC9A1; solute carrier family 9 member A1

model of autosomal recessive spinocerebellar ataxia 19

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

SLC9A3; solute carrier family 9 member A3

12 of 15

182307

DIARRHEA 8, SECRETORY SODIUM, CONGENITAL; DIAR8

congenital secretory sodium diarrhea 8

SLC9A5; solute carrier family 9 member A5

12 of 15

600477

SLC9A2; solute carrier family 9 member A2

6 of 15

600530

SLC9A1; solute carrier family 9 member A1

5 of 15

107310

LICHTENSTEIN-KNORR SYNDROME; LIKNS

autosomal recessive spinocerebellar ataxia 19

SLC9A4; solute carrier family 9 member A4

5 of 15

600531

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

View in Interactions Browser

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Nhe2 - fz

anti tag coimmunoprecipitation, anti tag western blot

(Simons et al., 2009)

Summary of Genetic Interactions

esyN Network Diagram

esyN Network Key:

Suppression

Enhancement

View in Interactions Browser

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Ae2

suppressible

Nhe2

(Benitez et al., 2019)

suppressible

Nhe2

(Simons et al., 2009)

ptc

suppressible

Nhe2

(Ulmschneider et al., 2016)

Raf

enhanceable

Nhe2

(Grillo-Hill et al., 2015)

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

61332

DroID - A comprehensive database of gene and protein interactions.

FBgn0040297

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

35376

MIST (protein-protein) - An integrated Molecular Interaction Database

Nhe2

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

External Data

Linkouts

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Sodium/Proton exchangers

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-54

Cytogenetic map

39A2-39A3

Sequence location

2L:21,104,568..21,134,255 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

39A2-39A3

Limits computationally determined from genome sequence between P{PZ}l(2)05287⁰⁵²⁸⁷ and P{lacW}l(2)k07215^k07215&P{lacW}snRNA:U4:39B^k09410

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

39A3-39B1

(Lin, 2000.2.16)

Experimentally Determined Recombination Data

Location

2-54

(FlyBase, 2016)

2-54.5

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (18)

Bloomington

51491

y¹ v¹; P{TRiP.HMC03243}attP2

65676

w¹¹¹⁸; PBac{IT.GAL4}Nhe2^1359-G4

36988

y¹ w^*; Mi{MIC}Nhe2^MI03660

37201

y¹ w^*; Mi{MIC}Nhe2^MI03496

37911

y¹ w^*; Mi{MIC}Nhe2^MI04913

43103

y¹ w^*; Mi{MIC}Nhe2^MI07256

44320

y¹ w^*; Mi{MIC}Nhe2^MI07035

55574

y¹ w^*; Mi{MIC}Nhe2^MI11025

65515

w¹¹¹⁸; PBac{IT.GAL4}Nhe2^1219-G4/CyO

20292

y¹ w^67c23; P{EPgy2}Nhe2^EY11323

VDRC

v330363

P{VSH330363}attP40

v106053

P{KK102518}VIE-260B

More stocks available...

Genomic Clones (31)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (18)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

FI19315

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0040297

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0040297

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

35376

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for database merge of

Source for merge of: NHE2 CG9256

(FlyBase, 1996-)

Source for merge of: NHE2 CG9255

(FlyBase Genome Annotators, 2002-2003)

Additional comments

One or more of the processed transcripts for these genes contain several non-overlapping open reading frames (ORFs). The non-overlapping ORFs are represented by CG46307 (FBgn0284223) and CG9256 (FBgn0040297).

(FlyBase Genome Annotators, 2016)

Annotations CG9256 and CG9255 merged as CG9256 in release 3 of the genome annotation.

(FlyBase Genome Annotators, 2002-2003)

Source for merge of NHE2 CG9256 was sequence comparison ( date:000401 ).

(FlyBase, 1996-)

Other Comments

ChEST reveals this is a target of Mef2.

(Junion et al., 2005)

Origin and Etymology

Discoverer

Etymology

Identification

External Crossreferences and Linkouts ( 99 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

35376

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0040297

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0040297

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG9256

Flygut - An atlas of the Drosophila adult midgut

FBgn0040297

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

35376

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC031052

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG9256

modMine - A data warehouse for the modENCODE project

FBgn0040297

Linkouts

BioGRID - A database of protein and genetic interactions.

61332

DroID - A comprehensive database of gene and protein interactions.

FBgn0040297

DRSC - Results frm RNAi screens

FBgn0040297

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0040297

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG9256-RA

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0040297

FlyMine - An integrated database for Drosophila genomics

FBgn0040297

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

35376

MIST (protein-protein) - An integrated Molecular Interaction Database

Nhe2

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Sodium/Proton exchangers

Synonyms and Secondary IDs (11)

Reported As

Symbol Synonym

CG9255

CG9256

(Meadows, 2017.1.11, Balakrishnan et al., 2015, Ni et al., 2010.12.1, Keleman et al., 2009.8.5, Simons et al., 2009)

DNhe2

(Ulmschneider et al., 2016)

DmNHE2

(Prasad and Rao, 2018, Brett et al., 2005, Brett et al., 2005, Giannakou and Dow, 2001, Giannikou et al., 2000)

Dnhe2

(Grillo-Hill et al., 2015)

NHE2

(Ghimire et al., 2019, Koh et al., 2004, Marie et al., 2004, Stanyon et al., 2004)

Nhe2

(Theis et al., 2020, Lee et al., 2018, Clandinin and Owens, 2016-, FlyBase Genome Annotators, 2016, Gene Disruption Project members, 2016-, Gene Disruption Project members, 2015-, Mackowiak et al., 2015, Ashwal-Fluss et al., 2014, Kwon et al., 2013, Petzoldt et al., 2013, Sen et al., 2013, Japanese National Institute of Genetics, 2012.5.21, Rodriguez et al., 2012, Simons et al., 2009)

dNhe2

(Benitez et al., 2019)

nhe2

(Overend et al., 2016, Day et al., 2008)

Name Synonyms

Drosophila sodium-proton exchanger 2

(Benitez et al., 2019)

Na+/H+ hydrogen exchanger 2

(Brett et al., 2005)

Nhe2

Secondary FlyBase IDs

FBgn0032917
FBgn0032918

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

References (70)

The following external sites may use different assemblies or annotations than FlyBase.

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