FB2022_03, released June 9, 2022

Gene: Dmel\Nhe2

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General Information
Symbol
Dmel\Nhe2
Species
D. melanogaster
Name
Na+/H+ hydrogen exchanger 2
Annotation Symbol
CG9256
Feature Type
protein_coding_gene
FlyBase ID
FBgn0040297
Gene Model Status
Current
Stock Availability
18 publicly available
Gene Summary
Na[+]/H[+] hydrogen exchanger 2 (Nhe2) encodes a Na-H exchanger of the SLC family 9A that increases intracellular pH. The product of Nhe2 activity promotes proliferation, morphogenesis and planar cell polarity, and contributes to oncogene-induced metastatic cell behaviors. [Date last reviewed: 2019-09-26] (FlyBase Gene Snapshot)
All Summaries
Also Known As

DmNHE2

Key Links
Genomic Location
Cytogenetic map
39A2-39A3
Sequence location
Recombination map
2-54
RefSeq locus
NT_033779 REGION:21104568..21134255
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
GO Summary Ribbons
Gene Ontology (GO) Annotations (13 terms)
Molecular Function (2 terms)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
enables potassium:proton antiporter activity
inferred from biological aspect of ancestor with PANTHER:PTN000011605
(assigned by GO_Central )
(Gaudet et al., 2011)
enables sodium:proton antiporter activity
inferred from biological aspect of ancestor with PANTHER:PTN000011605
(assigned by GO_Central )
(Gaudet et al., 2011)
Biological Process (8 terms)
Terms Based on Experimental Evidence (5 terms)
CV Term
Evidence
References
involved_in cellular ion homeostasis
inferred from expression pattern
(Day et al., 2008)
involved_in compound eye morphogenesis
inferred from mutant phenotype
(Grillo-Hill et al., 2015)
involved_in intracellular pH elevation
inferred from mutant phenotype
(Ulmschneider et al., 2016)
involved_in regulation of cellular pH
inferred from mutant phenotype
(Grillo-Hill et al., 2015)
involved_in renal system process
inferred from genetic interaction with FLYBASE:CG10939; FB:FBgn0010620
(Ghimire et al., 2019)
Terms Based on Predictions or Assertions (3 terms)
CV Term
Evidence
References
involved_in potassium ion transmembrane transport
inferred from biological aspect of ancestor with PANTHER:PTN000011605
(assigned by GO_Central )
(Gaudet et al., 2011)
involved_in regulation of intracellular pH
inferred from biological aspect of ancestor with PANTHER:PTN000011605
(assigned by GO_Central )
(Gaudet et al., 2011)
involved_in sodium ion import across plasma membrane
inferred from biological aspect of ancestor with PANTHER:PTN000011605
(assigned by GO_Central )
(Gaudet et al., 2011)
Cellular Component (3 terms)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
located_in apical plasma membrane
inferred from direct assay
(Ghimire et al., 2019)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
located_in integral component of membrane
inferred from electronic annotation with InterPro:IPR004709, InterPro:IPR006153, InterPro:IPR018422
(assigned by InterPro )
(FlyBase Curators et al., 2004-)
is_active_in plasma membrane
inferred from biological aspect of ancestor with PANTHER:PTN000011605
(assigned by GO_Central )
(Gaudet et al., 2011)
Gene Group (FlyBase)
Protein Family (UniProt)
-
Protein Signatures (InterPro)
Summaries
Gene Snapshot
Na[+]/H[+] hydrogen exchanger 2 (Nhe2) encodes a Na-H exchanger of the SLC family 9A that increases intracellular pH. The product of Nhe2 activity promotes proliferation, morphogenesis and planar cell polarity, and contributes to oncogene-induced metastatic cell behaviors. [Date last reviewed: 2019-09-26]
Gene Group (FlyBase)
SLC9 FAMILY OF SODIUM/PROTON EXCHANGERS -
The solute carrier family 9 (SLC9) members are Na+/H+ exchangers that maintain cellular pH by utilising the sodium gradient across the plasma membrane to extrude protons produced by metabolism. (Adapted from FBrf0229646).
Gene Model and Products
Number of Transcripts
12
Number of Unique Polypeptides
11

Please see the JBrowse view of Dmel\Nhe2 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Structure New Section
Protein 3D structure   (Predicted by AlphaFold)   (AlphaFold entry B7YZY2)

If you don't see the viewer to the right, refresh your browser.
Model Confidence:
  • Very high (pLDDT > 90)
  • Confident (90 > pLDDT > 70)
  • Low (70 > pLDDT > 50)
  • Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Comments on Gene Model

Gene model reviewed during 5.47

Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.

Low-frequency RNA-Seq exon junction(s) not annotated.

Annotated transcripts do not represent all supported alternative splices within 5' UTR.

Gene model reviewed during 5.53

Gene model reviewed during 6.13

Sequence Ontology: Class of Gene
gene_with_dicistronic_mRNA
(FlyBase Genome Annotators, 2016, Mackowiak et al., 2015)
gene_with_edited_transcript
(Rodriguez et al., 2012, Graveley et al., 2011)
nuclear_gene
protein_coding_gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
Nhe2-RA
FBtr0081466
NM_165355
4788
1291
Nhe2-RB
FBtr0081467
NM_136244
4794
1291
Nhe2-RC
FBtr0299908
NM_001144375
4578
1219
Nhe2-RD
FBtr0299909
NM_001144376
2838
672
Nhe2-RE
FBtr0299910
NM_001144377
4602
1229
Nhe2-RF
FBtr0299911
NM_001144378
2741
723
Nhe2-RG
FBtr0299912
NM_001144379
6233
1322
Nhe2-RH
FBtr0306015
NM_001259187
5898
1222
Nhe2-RI
FBtr0306016
NM_001259188
6466
1166
Nhe2-RJ
FBtr0334171
NM_001273731
4432
1305
Nhe2-RK
FBtr0334172
NM_001273732
4792
1177
Nhe2-RL
FBtr0334173
NM_001273733
4686
1257
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
UniProt
RefSeq ID
GenBank
Nhe2-PA
FBpp0080995
142.3
1291
6.84
Q9VIF9
NP_724311
AAF53960
Nhe2-PB
FBpp0080996
142.3
1291
6.84
Q9VIF9
NP_610088
AAF53961
Nhe2-PC
FBpp0289186
134.4
1219
7.10
B7YZY3
NP_001137847
ACL83053
Nhe2-PD
FBpp0289187
74.4
672
8.79
B7YZY5
NP_001137848
ACL83054
Nhe2-PE
FBpp0289188
135.6
1229
7.19
B7YZY1
NP_001137849
ACL83055
Nhe2-PF
FBpp0289189
80.4
723
9.33
B7YZY4
NP_001137850
ACL83056
Nhe2-PG
FBpp0289190
146.1
1322
7.46
B7YZY2
NP_001137851
ACL83057
Nhe2-PH
FBpp0297157
134.6
1222
8.98
M9NDU7
NP_001246116
AFH03790
Nhe2-PI
FBpp0297158
129.4
1166
9.06
M9NFD1
NP_001246117
AFH03791
Nhe2-PJ
FBpp0306288
143.9
1305
6.97
M9PDW6
NP_001260660
AGB93195
Nhe2-PK
FBpp0306289
129.7
1177
8.92
M9PDJ7
NP_001260661
AGB93196
Nhe2-PL
FBpp0306290
139.2
1257
7.43
M9PGE2
NP_001260662
AGB93197
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

1291 aa isoforms: Nhe2-PA, Nhe2-PB
Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Nhe2 using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that the Fly Cell Atlas project found the gene expressed in that cell type. Darker colors mean that more cells of that cell type express the gene:
 low
high 
Colorless tiles indicate that there is no scRNAseq data for the gene in that cell type.
Colored tiles in ribbon indicate that expression data (RNA and/or protein) has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
Colored tiles in the ribbon indicate the average RNA expression level of the gene at the indicated stages:
 low
high 
as determined by RNA-seq (RPKM) using whole organism samples modENCODE, Brown et al., 2014. For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 4 -- 6
organism | faint | ubiquitous
(Fisher et al., 2012)
embryonic stage 7 -- 8
head mesoderm
(Fisher et al., 2012)
trunk mesoderm
(Fisher et al., 2012)
embryonic stage 9 -- 10
antennal primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)
central brain primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)
visual primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)
dorsal head epidermis primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)
lateral head epidermis primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)
ventral head epidermis primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)
anterior endoderm
(Fisher et al., 2012)
head mesoderm
(Fisher et al., 2012)
posterior endoderm
(Fisher et al., 2012)
trunk mesoderm
(Fisher et al., 2012)
ventral nerve cord primordium
(Fisher et al., 2012)
embryonic stage 11 -- 12
anterior midgut primordium
(Fisher et al., 2012)
cardiogenic mesoderm
(Fisher et al., 2012)
central brain primordium
(Fisher et al., 2012)
dorsal pharyngeal muscle primordium
(Fisher et al., 2012)
embryonic central brain glial cell
(Fisher et al., 2012)
embryonic ganglion mother cell
(Fisher et al., 2012)
embryonic neuroblast of ventral nerve cord primordium
(Fisher et al., 2012)
fat body/gonad primordium
(Fisher et al., 2012)
garland cell primordium
(Fisher et al., 2012)
lateral cord glial cell
(Fisher et al., 2012)
lateral cord neuron
(Fisher et al., 2012)
plasmatocyte primordium

Comment: reported as plasmatocytes anlage

(Fisher et al., 2012)
posterior midgut primordium
(Fisher et al., 2012)
somatic muscle primordium
(Fisher et al., 2012)
trunk mesoderm
(Fisher et al., 2012)
embryonic stage 13 -- 16
embryonic/larval crystal cell
(Fisher et al., 2012)
dorsal pharyngeal muscle cell
(Fisher et al., 2012)
embryonic dorsal epidermis
(Fisher et al., 2012)
embryonic foregut
(Fisher et al., 2012)
embryonic/larval dorsal vessel
(Fisher et al., 2012)
embryonic/larval fat body
(Fisher et al., 2012)
embryonic/larval garland cell
(Fisher et al., 2012)
embryonic/larval midgut
(Fisher et al., 2012)
embryonic/larval muscle system
(Fisher et al., 2012)
embryonic/larval oenocyte
(Fisher et al., 2012)
embryonic/larval salivary gland
(Fisher et al., 2012)
embryonic/larval somatic muscle cell
(Fisher et al., 2012)
gonad
(Fisher et al., 2012)
embryonic/larval plasmatocyte
(Fisher et al., 2012)
larval ventral nerve cord
(Fisher et al., 2012)
microinjection
Stage
Tissue/Position (including subcellular localization)
Reference
larval stage
embryonic/larval midgut

Comment: enriched in acidic region

(Overend et al., 2016)
Additional Descriptive Data

RNA-seq data show that Nhe2 is expressed throughout the larval midgut but is enriched in the acidic region of the midgut.

(Overend et al., 2016)
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage | male
testis
(Day et al., 2008)
ejaculatory duct
(Day et al., 2008)
adult stage
adult copper cell
(Day et al., 2008)
adult Malpighian tubule
(Day et al., 2008)
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage
adult head
(Aradska et al., 2015)
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
located_in apical plasma membrane
inferred from direct assay
(Ghimire et al., 2019)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Nhe2 in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyExpress - Embryonic expression images (BDGP data)
  • Stages(s) 4-6
  • Stages(s) 7-8
  • Stages(s) 9-10
  • Stages(s) 11-12
  • Stages(s) 13-16
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 26 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 11 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of Nhe2
Transgenic constructs containing regulatory region of Nhe2
Aberrations (Deficiencies and Duplications) ( 2 )
Inferred from experimentation ( 2 )
Gene disrupted in
Df(2L)Exel6047
(Ryder, 2004.4.26)
Gene partially disrupted in
Df(2L)Nhe2null
(Grillo-Hill et al., 2015)
Inferred from location ( 0 )
Alleles Representing Disease-Implicated Variants
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
cell lethal | recessive | somatic clone
lethal - all die before end of larval stage | recessive
partially lethal - majority die
viable
viable, with Scer\GAL4Mef2.PR
viable (with Df(2L)BSC302)
Sterility
Allele
fertile
Other Phenotypes
Allele
abnormal cell shape | oogenesis | oogenesis
abnormal planar polarity, with Nhe21, Scer\GAL4hs.2sev
abnormal planar polarity, with Nhe2dsRNA.UAS.WIZ, Scer\GAL4hs.2sev
abnormal planar polarity, with Scer\GAL4hs.2sev
abnormal planar polarity (with Nhe21), with Scer\GAL4hs.2sev
abnormal planar polarity (with Nhe2dsRNA.UAS.WIZ), with Scer\GAL4hs.2sev
decreased cell number | oogenesis | oogenesis
decreased fecundity
increased cell number, with Scer\GAL4en.PU
increased occurrence of cell division | larval stage, with Scer\GAL4GMR.PU
increased occurrence of cell division | pupal stage, with Scer\GAL4GMR.PU
some die during larval stage | recessive
visible, with Scer\GAL4ey.3.5.Exel
visible, with Scer\GAL4GMR.PU
visible | adult stage, with Scer\GAL4GMR.PU
Phenotype manifest in
Allele
adult Malpighian tubule, with Scer\GAL4ClC-a.PU
basal stalk
basal stalk, with Scer\GAL4109-30
cone cell | pupal stage, with Scer\GAL4GMR.PU
embryonic/larval digestive system
embryonic/larval fat body
eye, with Nhe21, Scer\GAL4hs.2sev
eye, with Nhe2dsRNA.UAS.WIZ, Scer\GAL4hs.2sev
eye, with Scer\GAL4ey.3.5.Exel
eye, with Scer\GAL4GMR.PU
eye, with Scer\GAL4hs.2sev
eye (with Nhe21), with Scer\GAL4hs.2sev
eye (with Nhe2dsRNA.UAS.WIZ), with Scer\GAL4hs.2sev
eye disc posterior to the morphogenetic furrow, with Scer\GAL4GMR.PU
eye photoreceptor cell, with Scer\GAL4GMR.PU
eye photoreceptor cell, with Scer\GAL4hs.2sev
female germline cyst
female germline cyst, with Scer\GAL4109-30
female germline stem cell | oogenesis | oogenesis
female germline stem cell | oogenesis | oogenesis, with Scer\GAL4109-30
follicle cell
follicle cell, with Scer\GAL4109-30
germarium
germarium, with Scer\GAL4109-30
interommatidial bristle, with Scer\GAL4GMR.PU
ommatidium, with Nhe21, Scer\GAL4hs.2sev
ommatidium, with Nhe2dsRNA.UAS.WIZ, Scer\GAL4hs.2sev
ommatidium, with Scer\GAL4GMR.PU
ommatidium, with Scer\GAL4hs.2sev
ommatidium (with Nhe21), with Scer\GAL4hs.2sev
ommatidium (with Nhe2dsRNA.UAS.WIZ), with Scer\GAL4hs.2sev
ommatidium | pupal stage, with Scer\GAL4GMR.PU
primary pigment cell, with Scer\GAL4GMR.PU
retina
retina | pupal stage, with Scer\GAL4GMR.PU
secondary pigment cell
stalk follicle cell
stalk follicle cell, with Scer\GAL4109-30
wing disc posterior compartment, with Scer\GAL4en.PU
Orthologs
Downloads
Download All DIOPT Orthologs
Human Orthologs (via DIOPT v8.0)
Homo sapiens (Human) (13)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Hsap\SLC9A3
12 of 15
Yes
Yes
Hsap\SLC9A5
12 of 15
Yes
Yes
1  
Hsap\SLC9A2
6 of 15
No
Yes
Hsap\SLC9A1
5 of 15
No
Yes
Hsap\SLC9A4
5 of 15
No
Yes
Hsap\SLC9A9
2 of 15
No
No
Hsap\SLC9C1
2 of 15
No
Yes
Hsap\SLC9A6
1 of 15
No
No
Hsap\SLC9A7
1 of 15
No
No
Hsap\SLC9A8
1 of 15
No
No
Hsap\SLC9B1
1 of 15
No
No
Hsap\SLC9B2
1 of 15
No
No
Hsap\SLC9C2
1 of 15
No
Yes
Model Organism Orthologs (via DIOPT v8.0)
Mus musculus (laboratory mouse) (12)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Mmus\Slc9a5
12 of 15
Yes
Yes
Mmus\Slc9a3
10 of 15
No
Yes
Mmus\Slc9a2
6 of 15
No
Yes
Mmus\Slc9a1
5 of 15
No
Yes
Mmus\Slc9a4
5 of 15
No
Yes
Mmus\Slc9a9
2 of 15
No
No
Mmus\Slc9c1
2 of 15
No
Yes
Mmus\Slc9a6
1 of 15
No
No
Mmus\Slc9a7
1 of 15
No
No
Mmus\Slc9a8
1 of 15
No
No
Mmus\Slc9b1
1 of 15
No
No
Mmus\Slc9b2
1 of 15
No
No
Rattus norvegicus (Norway rat) (12)
Rnor\Slc9a3
9 of 13
Yes
Yes
Rnor\Slc9a5
8 of 13
No
Yes
Rnor\Slc9a1
5 of 13
No
Yes
Rnor\Slc9a2
5 of 13
No
Yes
Rnor\Slc9a4
4 of 13
No
Yes
Rnor\Slc9a9
2 of 13
No
No
Rnor\Slc9a6
1 of 13
No
No
Rnor\Slc9a7
1 of 13
No
No
Rnor\Slc9a8
1 of 13
No
No
Rnor\Slc9b1
1 of 13
No
No
Rnor\Slc9b2
1 of 13
No
No
Rnor\Slc9c1
1 of 13
No
Yes
Xenopus tropicalis (Western clawed frog) (11)
Xtro\slc9a5
7 of 12
Yes
Yes
Xtro\slc9a1
4 of 12
No
Yes
Xtro\LOC100488264
3 of 12
No
Yes
Xtro\slc9a3
2 of 12
No
Yes
Xtro\slc9a9
2 of 12
No
No
Xtro\slc9a2
1 of 12
No
Yes
Xtro\slc9a4
1 of 12
No
Yes
Xtro\slc9a6
1 of 12
No
No
Xtro\slc9a7
1 of 12
No
No
Xtro\slc9a8
1 of 12
No
No
Xtro\slc9b2
1 of 12
No
No
Danio rerio (Zebrafish) (10)
Drer\slc9a3.1
10 of 15
Yes
Yes
Drer\slc9a5
8 of 15
No
Yes
Drer\slc9a3.2
7 of 15
No
Yes
Drer\slc9a2
5 of 15
No
Yes
Drer\slc9a1b
2 of 15
No
Yes
Drer\si:dkey-162b23.4
1 of 15
No
No
Drer\slc9a1a
1 of 15
No
Yes
Drer\slc9a6a
1 of 15
No
No
Drer\slc9a6b
1 of 15
No
No
Drer\slc9a8
1 of 15
No
No
Caenorhabditis elegans (Nematode, roundworm) (11)
Cele\nhx-3
10 of 15
Yes
Yes
Cele\nhx-2
9 of 15
No
Yes
Cele\nhx-9
9 of 15
No
Yes
Cele\nhx-6
8 of 15
No
Yes
Cele\nhx-1
5 of 15
No
Yes
Cele\nhx-4
3 of 15
No
Yes
Cele\pbo-4
3 of 15
No
Yes
Cele\F41E7.1
1 of 15
No
No
Cele\F57G8.5
1 of 15
No
No
Cele\nhx-5
1 of 15
No
No
Cele\nhx-8
1 of 15
No
No
Arabidopsis thaliana (thale-cress) (6)
Atha\NHX1
1 of 9
Yes
No
Atha\NHX2
1 of 9
Yes
No
Atha\NHX3
1 of 9
Yes
No
Atha\NHX4
1 of 9
Yes
No
Atha\NHX5
1 of 9
Yes
No
Atha\NHX6
1 of 9
Yes
No
Saccharomyces cerevisiae (Brewer's yeast) (2)
Scer\NHX1
2 of 15
Yes
No
Scer\NHA1
1 of 15
No
Yes
Schizosaccharomyces pombe (Fission yeast) (1)
Spom\SPAC15A10.06
1 of 12
Yes
No
Other Organism Orthologs (via OrthoDB)
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v8.0)
Drosophila melanogaster (Fruit fly) (4)
Gene Symbol
Score
Source
Compara
eggNOG
Homologene
Inparanoid
Isobase
OrthoDB
OrthoFinder
orthoMCL
Panther
RoundUp
Alignment
Nhe1
3 of 10
Nhe3
3 of 10
Nha1
1 of 10
Nha2
1 of 10
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Model Summary Ribbon
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 1 )
    Allele
    Disease
    Evidence
    References
    Nhe2KK102518
    model of  nephrolithiasis
    CEA
    (Ghimire et al., 2019)
    Potential Models Based on Orthology ( 2 )
    Modifiers Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Interaction
    References
    Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    SLC9A3; solute carrier family 9 member A3
    12 of 15
    182307
    SLC9A5; solute carrier family 9 member A5
    12 of 15
    600477
         
        SLC9A2; solute carrier family 9 member A2
        6 of 15
        600530
            SLC9A1; solute carrier family 9 member A1
            5 of 15
            107310
            SLC9A4; solute carrier family 9 member A4
            5 of 15
            600531
                Functional Complementation Data
                Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
                Interactions
                Summary of Physical Interactions
                esyN Network Diagram
                Show neighbor-neighbor interactions:
                Select Layout:
                Legend:
                Protein
                RNA
                Selected Interactor(s)
                Interactions Browser
                View in Interactions Browser
                Please see the Physical Interaction reports below for full details
                protein-protein
                Physical Interaction
                Assay
                References
                Nhe2 - fz
                anti tag coimmunoprecipitation, anti tag western blot
                (Simons et al., 2009)
                Summary of Genetic Interactions
                esyN Network Diagram
                esyN Network Key:
                Suppression
                Enhancement
                View in Interactions Browser
                Please look at the allele data for full details of the genetic interactions
                Starting gene(s)
                Interaction type
                Interacting gene(s)
                Reference
                Starting gene(s)
                Interaction type
                Interacting gene(s)
                Reference
                Ae2
                suppressible
                Nhe2
                (Benitez et al., 2019)
                fz
                suppressible
                Nhe2
                (Simons et al., 2009)
                ptc
                suppressible
                Nhe2
                (Ulmschneider et al., 2016)
                Raf
                enhanceable
                Nhe2
                (Grillo-Hill et al., 2015)
                External Data
                Linkouts
                BioGRID - A database of protein and genetic interactions.
                DroID - A comprehensive database of gene and protein interactions.
                MIST (protein-protein) - An integrated Molecular Interaction Database
                Pathways
                Signaling Pathways (FlyBase)
                Metabolic Pathways
                FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.
                External Data
                Linkouts
                Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
                Genomic Location and Detailed Mapping Data
                Chromosome (arm)
                2L
                Recombination map
                2-54
                Cytogenetic map
                39A2-39A3
                Sequence location
                FlyBase Computed Cytological Location
                Cytogenetic map
                Evidence for location
                39A2-39A3
                Limits computationally determined from genome sequence between P{PZ}l(2)0528705287 and P{lacW}l(2)k07215k07215&P{lacW}snRNA:U4:39Bk09410
                Experimentally Determined Cytological Location
                Cytogenetic map
                Notes
                References
                39A3-39B1
                (Lin, 2000.2.16)
                Experimentally Determined Recombination Data
                Location

                2-54

                (FlyBase, 2016)

                2-54.5

                (Comeron et al., 2012)
                Left of (cM)
                Right of (cM)
                Notes
                Stocks and Reagents
                Stocks (18)
                Genomic Clones (31)
                cDNA Clones (18)
                 

                Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

                cDNA clones, fully sequenced
                BDGP DGC clones
                Other clones
                Drosophila Genomics Resource Center cDNA clones

                For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

                cDNA Clones, End Sequenced (ESTs)
                BDGP DGC clones
                Other clones
                RNAi and Array Information
                Linkouts
                DRSC - Results frm RNAi screens
                GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
                Antibody Information
                Laboratory Generated Antibodies
                 
                Commercially Available Antibodies
                 
                Other Information
                Relationship to Other Genes
                Source for database identify of
                Source for database merge of

                Source for merge of: NHE2 CG9256

                (FlyBase, 1996-)

                Source for merge of: NHE2 CG9255

                (FlyBase Genome Annotators, 2002-2003)
                Additional comments

                One or more of the processed transcripts for these genes contain several non-overlapping open reading frames (ORFs). The non-overlapping ORFs are represented by CG46307 (FBgn0284223) and CG9256 (FBgn0040297).

                (FlyBase Genome Annotators, 2016)

                Annotations CG9256 and CG9255 merged as CG9256 in release 3 of the genome annotation.

                (FlyBase Genome Annotators, 2002-2003)

                Source for merge of NHE2 CG9256 was sequence comparison ( date:000401 ).

                (FlyBase, 1996-)
                Other Comments

                ChEST reveals this is a target of Mef2.

                (Junion et al., 2005)
                Origin and Etymology
                Discoverer
                Etymology
                Identification
                External Crossreferences and Linkouts ( 104 )
                Sequence Crossreferences
                NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
                GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
                GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
                RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
                UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.
                UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
                Other crossreferences
                AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.
                BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
                Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
                DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
                EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
                Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
                FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
                FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
                Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
                Flygut - An atlas of the Drosophila adult midgut
                FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.
                GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
                iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
                InterPro - A database of protein families, domains and functional sites
                KEGG Genes - Molecular building blocks of life in the genomic space.
                MARRVEL_MODEL - MARRVEL (model organism gene)
                modMine - A data warehouse for the modENCODE project
                Linkouts
                BioGRID - A database of protein and genetic interactions.
                DroID - A comprehensive database of gene and protein interactions.
                DRSC - Results frm RNAi screens
                FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
                FlyMine - An integrated database for Drosophila genomics
                MIST (protein-protein) - An integrated Molecular Interaction Database
                Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
                Synonyms and Secondary IDs (11)
                Datasets (0)
                Study focus (0)
                Experimental Role
                Project
                Project Type
                Title
                Study result (0)
                Result
                Result Type
                Title
                References (73)