FlyBase Gene Report: Dmel\MTF-1

General Information

Symbol

Dmel\MTF-1

Species

D. melanogaster

Name

Metal response element-binding Transcription Factor-1

Annotation Symbol

CG3743

Feature Type

protein_coding_gene

FlyBase ID

FBgn0040305

Gene Model Status

Current

Stock Availability

16 publicly available

Gene Summary

Metal response element-binding Transcription Factor-1 (MTF-1) encodes a zinc finger protein that primarily controls the homeostasis of physiological trace metals, such as zinc and copper, and the detoxification of non-essential, toxic heavy metals, such as cadmium, mercury, and silver. It activates a number of genes in response to heavy metal load but also induces transcription of the copper importer encoded by Ctr1B upon copper depletion. Variations in the DNA binding site can affect its response to a specific metal. [Date last reviewed: 2019-03-14] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
Interactive Fly

Also Known As

dMTF-1, metal responsive transcription factor

Key Links

Genomic Location

Cytogenetic map

67B5-67B6

Sequence location

3L:9,430,312..9,439,490 [+]

Recombination map

3-29

RefSeq locus

NT_037436 REGION:9430312..9439490

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse JBrowse2 (beta)

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (10 terms)

Molecular Function (2 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

enables DNA-binding transcription factor activity

inferred from direct assay

(Marr et al., 2006)

enables transcription cis-regulatory region binding

inferred from direct assay

(Atanesyan et al., 2011)

Terms Based on Predictions or Assertions (0 terms)

Biological Process (6 terms)

Terms Based on Experimental Evidence (5 terms)

CV Term

Evidence

References

involved_in cellular response to oxidative stress

inferred from mutant phenotype

(Bahadorani et al., 2010)

involved_in positive regulation of transcription by RNA polymerase II

inferred from direct assay

(Marr et al., 2006)

involved_in response to copper ion

inferred from mutant phenotype

(Southon et al., 2004)

involved_in response to metal ion

inferred from mutant phenotype

(Selvaraj et al., 2005, Egli et al., 2003)

involved_in stress response to metal ion

inferred from mutant phenotype

(Bahadorani et al., 2010)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

involved_in regulation of transcription by RNA polymerase II

inferred from biological aspect of ancestor with PANTHER:PTN000578629

(GO Reference Genome Project, 2011-)

Cellular Component (2 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

located_in cytoplasm

inferred from direct assay

(Marr et al., 2006)

inferred from high throughput direct assay

(Sarov et al., 2016)

located_in nucleus

inferred from direct assay

(Marr et al., 2006)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

is_active_in nucleus

inferred from biological aspect of ancestor with PANTHER:PTN000578629

(GO Reference Genome Project, 2011-)

Gene Group / Complex (FlyBase)

C2H2 ZINC FINGER TRANSCRIPTION FACTORS

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

Gene Group / Complex (FlyBase)

C2H2 ZINC FINGER TRANSCRIPTION FACTORS -: Zinc finger C2H2 transcription factors are sequence-specific DNA binding proteins that regulate transcription. They possess DNA-binding domains that are formed from repeated Cys₂His₂ zinc finger motifs. (Adapted from PMID:1835093, FBrf0220103 and FBrf0155739).

Summary (Interactive Fly)

zinc finger transcription factor known familiarly as metal transcription factor - controls heavy metal detoxification

Interactive Fly

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\MTF-1 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry A8JNP3)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Annotated transcripts do not represent all supported alternative splices within 5' UTR.

Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.

Gene model reviewed during 5.45

Gene model reviewed during 5.53

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

MTF-1-RA

FBtr0076459

NM_140054

3375

791

MTF-1-RB

FBtr0076460

NM_168343

3272

570

MTF-1-RC

FBtr0113322

NM_001104090

4020

1006

MTF-1-RD

FBtr0113323

NM_001104091

3872

570

MTF-1-RE

FBtr0113324

NM_001104092

2812

570

MTF-1-RF

FBtr0344236

NM_001300073

3182

822

MTF-1-RG

FBtr0344237

NM_001300074

3523

570

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

MTF-1-PA

FBpp0076187

85.1

791

6.46

FBpp0076188

60.1

570

5.66

FBpp0112234

108.6

1006

4.89

FBpp0112235

60.1

570

5.66

FBpp0112236

60.1

570

5.66

FBpp0310640

90.9

822

4.63

FBpp0310641

60.1

570

5.66

Q8IQC5

NP_001287003

AHN58028

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

570 aa isoforms: MTF-1-PB, MTF-1-PD, MTF-1-PE, MTF-1-PG

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\MTF-1 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.95

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism | intense | ubiquitous

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 7 -- 8

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 9 -- 10

garland cell primordium

(Fisher et al., 2012)

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 11 -- 12

anterior midgut primordium

(Fisher et al., 2012)

garland cell primordium

(Fisher et al., 2012)

organism | ubiquitous

(Fisher et al., 2012)

posterior midgut primordium

(Fisher et al., 2012)

embryonic stage 13 -- 16

embryonic hindgut

(Fisher et al., 2012)

embryonic/larval garland cell

(Fisher et al., 2012)

embryonic/larval midgut

(Fisher et al., 2012)

larval muscle system

(Fisher et al., 2012)

larval visceral muscle cell

(Fisher et al., 2012)

organism | faint | ubiquitous

(Fisher et al., 2012)

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage -- larval stage

organism | faint

(Zhang et al., 2001)

pupal stage

organism | intense

(Zhang et al., 2001)

Additional Descriptive Data

MTF-1 transcript is expressed at a low level during embryonic development (pooled 0-20 hr AEL embryos), with expression levels increasing slightly during larval and strongly during pupal stages.

(Zhang et al., 2001)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in cytoplasm

inferred from direct assay

(Marr et al., 2006)

inferred from high throughput direct assay

(Sarov et al., 2016)

located_in nucleus

inferred from direct assay

(Marr et al., 2006)

Expression Deduced from Reporters

Reporter: P{Switch2}MTF-1^GSGB3b1-3

Stage

Tissue/Position (including subcellular localization)

Reference

third instar larval stage

larval somatic muscle cell

(Nicholson et al., 2008)

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\MTF-1 in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\MTF-1

View exonic expression by tissue for Dmel\MTF-1

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0040305

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

39089/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0040305

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0040305

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0040305

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG3743

Flygut - An atlas of the Drosophila adult midgut

FBgn0040305

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\MTF-1

Stages(s) 1-3
Stages(s) 4-6
Stages(s) 11-12
Stages(s) 13-16

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 10 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 28 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of MTF-1

Transgenic constructs containing regulatory region of MTF-1

Aberrations (Deficiencies and Duplications) ( 1 )

Inferred from experimentation ( 1 )

Gene disrupted in

Df(3L)AC1

(Egli et al., 2003)

Inferred from location ( 1 )

Dp(3;2)GV-CH321-17F07

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal - all die before end of larval stage

MTF-1^140-1R

partially lethal - majority die

partially lethal - majority die, with Scer\GAL4^pnr-MD237

MTF-1^GD4114

viable

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

MTF-1^GD4114

viable, with Scer\GAL4^Mef2.PR

MTF-1^GD4114

viable, with Scer\GAL4^tin.CΔ4

MTF-1^GD4114

viable (with Df(3L)AC1)

Sterility

Allele

female sterile

fertile

fertile (with Df(3L)AC1)

Other Phenotypes

Allele

abnormal developmental rate

MTF-1^140-1R

abnormal oxidative stress response, with Scer\GAL4^Toll-6-D42

MTF-1^UAS.cEa

abnormal starvation stress response

MTF-1^Tub.PY

abnormal starvation stress response, with Scer\GAL4^bbg-C96

MTF-1^UAS.cEa

abnormal starvation stress response, with Scer\GAL4^Toll-6-D42

MTF-1^UAS.cEa

chemical resistant

MTF-1^Tub.PY

chemical resistant, with MTF-1^140-1R, Scer\GAL4^NP2020

MTF-1^UAS.cEa

chemical resistant, with MTF-1^UAS.cEa, Scer\GAL4^NP2020

MTF-1^140-1R

chemical sensitive

chemical sensitive, with MTF-1^140-1R, Scer\GAL4^NP2020

MTF-1^UAS.cEa

chemical sensitive, with MTF-1^140-1R, Scer\GAL4^NP6219

MTF-1^UAS.cEa

chemical sensitive, with MTF-1^UAS.cEa, Scer\GAL4^NP2020

MTF-1^140-1R

chemical sensitive, with MTF-1^UAS.cEa, Scer\GAL4^NP6219

MTF-1^140-1R

chemical sensitive, with Scer\GAL4^NP2020

MTF-1^UAS.cEa

chemical sensitive, with Scer\GAL4^Toll-6-D42

MTF-1^UAS.cEa

chemical sensitive | recessive

MTF-1^140-1R

long lived | chemical conditional, with Scer\GAL4^bbg-C96

MTF-1^UAS.cEa

long lived | chemical conditional, with Scer\GAL4^Toll-6-D42

MTF-1^UAS.cEa

short lived

short lived, with Scer\GAL4^NP6219

MTF-1^UAS.cEa

short lived | calorie restriction conditional, with Scer\GAL4^bbg-C96

MTF-1^UAS.cEa

short lived | calorie restriction conditional, with Scer\GAL4^Toll-6-D42

MTF-1^UAS.cEa

short lived | chemical conditional

short lived | chemical conditional, with MTF-1^140-1R, Scer\GAL4^NP2020

MTF-1^UAS.cEa

short lived | chemical conditional, with MTF-1^140-1R, Scer\GAL4^NP6219

MTF-1^UAS.cEa

short lived | chemical conditional, with MTF-1^UAS.cEa, Scer\GAL4^NP2020

MTF-1^140-1R

short lived | chemical conditional, with MTF-1^UAS.cEa, Scer\GAL4^NP6219

MTF-1^140-1R

short lived | chemical conditional, with Scer\GAL4^NP2020

MTF-1^UAS.cEa

short lived | chemical conditional, with Scer\GAL4^Toll-6-D42

MTF-1^UAS.cEa

some die during pupal stage, with Scer\GAL4^pnr-MD237

MTF-1^GD4114

some die during third instar larval stage

MTF-1^140-1R

visible

visible, with Scer\GAL4^pnr-MD237

MTF-1^GD4114

Phenotype manifest in

Allele

mesonotum, with Scer\GAL4^pnr-MD237

MTF-1^GD4114

wing

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (218)

Hsap\MTF1

7 of 14

Yes

Hsap\GLIS1

2 of 14

Hsap\GLIS2

2 of 14

Hsap\ZNF155

2 of 14

Yes

Hsap\ZNF223

2 of 14

Yes

Hsap\ZNF230

2 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Hsap\KLF1

1 of 14

Hsap\LOC344065

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Hsap\ZNF225

1 of 14

Hsap\ZNF232

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Hsap\ZNF414

1 of 14

Yes

Hsap\ZNF417

1 of 14

Hsap\ZNF419

1 of 14

Yes

Hsap\ZNF426

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Hsap\ZNF653

1 of 14

Yes

Hsap\ZNF658B

1 of 14

Hsap\ZNF658

1 of 14

Hsap\ZNF660

1 of 14

Hsap\ZNF660-ZNF197

1 of 14

Hsap\ZNF664

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Hsap\ZXDA

1 of 14

Hsap\ZXDB

1 of 14

Hsap\ZXDC

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (75)

Rnor\Mtf1

6 of 14

Yes

Rnor\LOC102548695

2 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Rnor\LOC102546648

1 of 14

Rnor\LOC102547287

1 of 14

Rnor\LOC102556092

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Rnor\Zfp108

1 of 14

Rnor\Zfp143

1 of 14

Rnor\Zfp174

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Rnor\Zfp263

1 of 14

Rnor\Zfp287

1 of 14

Rnor\Zfp300

1 of 14

Yes

Rnor\Zfp317

1 of 14

Rnor\Zfp354c

1 of 14

Rnor\Zfp358

1 of 14

Yes

Rnor\Zfp367

1 of 14

Yes

Rnor\Zfp410

1 of 14

Yes

Rnor\Zfp414

1 of 14

Yes

Rnor\Zfp420

1 of 14

Rnor\Zfp523

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Rnor\Zfp697

1 of 14

Yes

Rnor\Zfp709

1 of 14

Rnor\Zfp764l1

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Rnor\Znf8

1 of 14

Rnor\Zscan2

1 of 14

Rnor\Zscan22

1 of 14

Yes

Rnor\Zxdc

1 of 14

Mus musculus (laboratory mouse) (113)

Mmus\Mtf1

6 of 14

Yes

Mmus\Zfp61

2 of 14

Yes

Mmus\Zfp93

2 of 14

Yes

Mmus\Zfp108

2 of 14

Yes

Mmus\Zfp109

2 of 14

Yes

Mmus\Zfp235

2 of 14

Yes

Mmus\A430033K04Rik

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Mmus\Gm14401

1 of 14

Yes

Mmus\Gm14403

1 of 14

Yes

Mmus\Gm14409

1 of 14

Mmus\Gm14418

1 of 14

Mmus\Gm14430

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Mmus\Zfp1

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Mmus\Zfp276

1 of 14

Yes

Mmus\Zfp282

1 of 14

Yes

Mmus\Zfp287

1 of 14

Yes

Mmus\Zfp317

1 of 14

Mmus\Zfp324

1 of 14

Mmus\Zfp341

1 of 14

Yes

Mmus\Zfp358

1 of 14

Mmus\Zfp367

1 of 14

Yes

Mmus\Zfp410

1 of 14

Yes

Mmus\Zfp414

1 of 14

Yes

Mmus\Zfp420

1 of 14

Mmus\Zfp523

1 of 14

Yes

Mmus\Zfp560

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Mmus\Zfp658

1 of 14

Mmus\Zfp689

1 of 14

Yes

Mmus\Zfp692

1 of 14

Yes

Mmus\Zfp697

1 of 14

Yes

Mmus\Zfp760

1 of 14

Mmus\Zfp771

1 of 14

Mmus\Zfp772

1 of 14

Yes

Mmus\Zfp872

1 of 14

Yes

Mmus\Zfp879

1 of 14

Mmus\Zfp931

1 of 14

Yes

Mmus\Zfp935

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Mmus\Zkscan8

1 of 14

Yes

Mmus\Zscan2

1 of 14

Mmus\Zxdc

1 of 14

Xenopus tropicalis (Western clawed frog) (66)

Xtro\mtf1

4 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Xtro\LOC100498327

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Xtro\rest

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Xtro\znf229

1 of 13

Xtro\znf282

1 of 13

Xtro\znf292

1 of 13

Yes

Xtro\znf367

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Danio rerio (Zebrafish) (133)

Drer\mtf1

5 of 14

Yes

Drer\aebp2

1 of 14

Drer\bif1.1

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Drer\LOC100329844

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Drer\LOC101883936

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Drer\LOC108183899

1 of 14

Yes

Drer\LOC108183927

1 of 14

Yes

Drer\LOC110437746

1 of 14

Drer\LOC110437786

1 of 14

Yes

Drer\LOC110437815

1 of 14

Drer\LOC110437819

1 of 14

Yes

Drer\LOC110437839

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Drer\rreb1a

1 of 14

Drer\si:cabz01021430.2

1 of 14

Yes

Drer\si:cabz01069022.1

1 of 14

Yes

Drer\si:ch73-138e16.3

1 of 14

Yes

Drer\si:ch73-144d13.4

1 of 14

Yes

Drer\si:ch73-367f21.6

1 of 14

Drer\si:ch211-79k12.2

1 of 14

Yes

Drer\si:ch211-119o8.6

1 of 14

Yes

Drer\si:ch211-142b24.6

1 of 14

Yes

Drer\si:ch211-161m3.6

1 of 14

Drer\si:ch211-223a21.4

1 of 14

Yes

Drer\si:ch211-229l10.11

1 of 14

Drer\si:ch211-241b2.1

1 of 14

Yes

Drer\si:ch211-241e1.5

1 of 14

Yes

Drer\si:dkey-14d8.1

1 of 14

Yes

Drer\si:dkey-30f3.2

1 of 14

Yes

Drer\si:dkey-40j3.3

1 of 14

Yes

Drer\si:dkey-182i3.8

1 of 14

Drer\si:dkey-182i3.9

1 of 14

Yes

Drer\si:dkey-201g16.1

1 of 14

Yes

Drer\si:dkey-262g12.3

1 of 14

Yes

Drer\si:dkey-262g12.9

1 of 14

Yes

Drer\si:dkeyp-113d7.1

1 of 14

Yes

Drer\si:rp71-1h20.5

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Drer\zgc:113209

1 of 14

Drer\zgc:113343

1 of 14

Drer\zgc:162971

1 of 14

Yes

Drer\zgc:162972

1 of 14

Drer\zgc:171220

1 of 14

Yes

Drer\zgc:171422

1 of 14

Yes

Drer\zgc:171435

1 of 14

Drer\zgc:171599

1 of 14

Yes

Drer\zgc:172128

1 of 14

Drer\zgc:173486

1 of 14

Yes

Drer\zgc:173619

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Drer\znf143a

1 of 14

Yes

Drer\znf143b

1 of 14

Yes

Drer\znf367

1 of 14

Drer\znf410

1 of 14

Yes

Drer\znf414

1 of 14

Yes

Drer\znf526

1 of 14

Yes

Drer\znf569l

1 of 14

Drer\znf653

1 of 14

Yes

Drer\znf668

1 of 14

Yes

Drer\znf971

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Drer\znf1158

1 of 14

Drer\znf1161

1 of 14

Yes

Drer\znf1168

1 of 14

Yes

Drer\znf1179

1 of 14

Yes

Drer\znf1184

1 of 14

Yes

Caenorhabditis elegans (Nematode, roundworm) (9)

Cele\lin-29

1 of 14

Yes

Cele\pag-3

1 of 14

Yes

Cele\pat-9

1 of 14

Yes

Cele\ref-2

1 of 14

Yes

Cele\scrt-1

1 of 14

Yes

Cele\tra-1

1 of 14

Yes

Cele\Y5F2A.4

1 of 14

Yes

Cele\znf-782

1 of 14

Yes

Cele\ztf-14

1 of 14

Yes

Anopheles gambiae (African malaria mosquito) (41)

Agam\AgaP_AGAP007377

10 of 12

Yes

Agam\AgaP_AGAP000286

1 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Yes

Agam\AgaP_AGAP004637

1 of 12

Agam\AgaP_AGAP004729

1 of 12

Agam\AgaP_AGAP004764

1 of 12

Yes

Agam\AgaP_AGAP005348

1 of 12

Agam\AgaP_AGAP005390

1 of 12

Agam\AgaP_AGAP005570

1 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Yes

Agam\AgaP_AGAP008901

1 of 12

Agam\AgaP_AGAP009566

1 of 12

Agam\AgaP_AGAP009764

1 of 12

Yes

Agam\AgaP_AGAP009889

1 of 12

Agam\AgaP_AGAP010516

1 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

Yes

Agam\AgaP_AGAP012594

1 of 12

Agam\AgaP_AGAP012834

1 of 12

Yes

Agam\AgaP_AGAP013536

1 of 12

Arabidopsis thaliana (thale-cress) (7)

Atha\AT5G22890

1 of 13

Yes

Atha\DOT5

1 of 13

Yes

Atha\IDD14

1 of 13

Yes

Atha\MGP

1 of 13

Yes

Atha\STOP1

1 of 13

Yes

Atha\WIP4

1 of 13

Yes

Atha\YY1

1 of 13

Yes

Saccharomyces cerevisiae (Brewer's yeast) (3)

Scer\AZF1

1 of 13

Yes

Scer\PZF1

1 of 13

Yes

Scer\ZAP1

1 of 13

Yes

Schizosaccharomyces pombe (Fission yeast) (0)

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:MTF-1. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (60)

2 of 13

2 of 13

2 of 13

2 of 13

esg

2 of 13

pad

2 of 13

Phs

2 of 13

wdn

2 of 13

BEAF-75

1 of 13

BroN

1 of 13

Cf2

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

dwg

1 of 13

hkb

1 of 13

jing

1 of 13

Kah

1 of 13

Kr-h1

1 of 13

l(3)neo38

1 of 13

lmd

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

pho

1 of 13

prg

1 of 13

rgr

1 of 13

Sag1

1 of 13

scrape

1 of 13

scrt

1 of 13

sna

1 of 13

sqz

1 of 13

su(Hw)

1 of 13

sug

1 of 13

wek

1 of 13

wor

1 of 13

Zaf1

1 of 13

ZIPIC

1 of 13

zld

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 7 )

Allele

Disease

Interaction

References

MTF-1^140-1R

ameliorates Friedreich ataxia 1

modeled by fh^RNAi.UAS.cAa

(Soriano et al., 2016)

exacerbates Parkinson's disease

modeled by park²⁵

(Saini et al., 2011)

MTF-1^unspecified

exacerbates Alzheimer's disease

modeled by Hsap\APP^{Aβ42.UAS.Tag:SS(hh)}

(Hua et al., 2011)

MTF-1^UAS.cSa

ameliorates Parkinson's disease

modeled by park²⁵

(Saini et al., 2011)

MTF-1^αTub84B.PS

ameliorates Parkinson's disease

modeled by park²⁵

(Saini et al., 2011)

MTF-1^Tub.PY

ameliorates Friedreich ataxia 1

modeled by fh^RNAi.UAS.cAa

(Soriano et al., 2016)

ameliorates Menkes disease

modeled by ATP7^RNAi.Sym.UAS

(Bahadorani et al., 2010)

MTF-1^UAS.cHa

ameliorates Alzheimer's disease

modeled by Hsap\APP^{Aβ42.UAS.Tag:SS(hh)}

(Hua et al., 2011)

MTF-1^EY03895

ameliorates Friedreich ataxia 1

modeled by fh^RNAi.UAS.cAa

(Soriano et al., 2016)

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

MTF1; metal regulatory transcription factor 1

7 of 14

600172

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Dmel gene

Ortholog showing functional complementation

Supporting References

MTF-1

Hsap\MTF1

(Balamurugan et al., 2004)

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

MTF-1 - MED17

pull down, western blot

(Marr et al., 2012)

MTF-1 - MED26

pull down, western blot

(Marr et al., 2012)

MTF-1 - Tbp

pull down, western blot

(Marr et al., 2012)

MTF-1 - gw

coimmunoprecipitation, western blot, anti tag coimmunoprecipitation, anti tag western blot

(Jia et al., 2021)

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

MTF-1

suppressible

CG10505

(Yepiskoposyan et al., 2006)

MTF-1

suppressible

Ctr1B

(Günther et al., 2012, Selvaraj et al., 2005)

MTF-1

enhanceable

MtnA|MtnB|MtnC|MtnD

(Günther et al., 2012)

MTF-1

suppressible

ZnT35C

(Yepiskoposyan et al., 2006)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

ATP7

suppressible

MTF-1

(Bahadorani et al., 2010)

Ctr1B

suppressible

MTF-1

(Egli et al., 2006)

Dpy-30L1

suppressible

MTF-1

(Vardanyan et al., 2008)

park

suppressible

MTF-1

(Saini et al., 2011)

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

64482

DroID - A comprehensive database of gene and protein interactions.

FBgn0040305

MIST (genetic) - An integrated Molecular Interaction Database

39089

MIST (protein-protein) - An integrated Molecular Interaction Database

39089

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-29

Cytogenetic map

67B5-67B6

Sequence location

3L:9,430,312..9,439,490 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

67B5-67B6

Limits computationally determined from genome sequence between P{EP}Hsp26^EP3336&P{EP}Hsp26^EP3315 and P{PZ}fry⁰²²⁴⁰

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

3-29

(FlyBase, 2016)

3-23.0

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (16)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

9241

MTF-1^140-1R

59954

w^*; P{Switch2}MTF-1^GSGB3b1-3

64808

y¹ w^*; P{MTF-1-GFP.FPTB}attP40

52986

y¹ v¹; P{TRiP.HMJ21677}attP40/CyO

16580

y¹ w^67c23; P{EPgy2}MTF-1^EY03895

86118

w¹¹¹⁸; PBac{WH}MTF-1^f07255/TM6B, Tb¹

80188

y¹ sc^* v¹ sev²¹; P{TOE.GS02503}attP40

FlyORF

F001779

M{UAS-MTF-1.ORF.3xHA.GW}ZH-86Fb

VDRC

v107124

P{KK113730}VIE-260B

v30455

w¹¹¹⁸; P{GD4114}v30455

v318883

PBac{fTRG00308.sfGFP-TVPTBF}VK00033

v342394

P{hsFLP}1, y¹ w¹¹¹⁸; P{HD_CFD00471}attP40/CyO-GFP

More stocks available...

Genomic Clones (25)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (27)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

FI01022

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0040305

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0040305

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Sims et al., 2012)

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

Gene expression is increased in response to the presence of two copies of Scer\GAL4^hs.PB.

(Liu and Lehmann, 2008)

Regulation of Ctr1B upon copper starvation is lost in the absence of MTF-1.

(Selvaraj et al., 2005)

dsRNA made from templates generated with primers directed against this gene used to investigate the role of this gene in copper homoeostasis in S2 cells.

(Southon et al., 2004)

S2 cells treated with MTF-1 dsRNA show reduced tolerance to increased copper in the culture medium.

(Southon et al., 2004)

MTF-1 is essential for the control of heavy metal homeostasis.

(Egli et al., 2003)

Area matching Drosophila EST AI532170. Forms a 1565bp EST contig with AI544333 and AI062662, sequence similarity to MTF-1 gene. Probable intron in gene represented by EST AI532170.

(Davies, 2001.3.15)

Relationship to Other Genes

Source for database merge of

Source for merge of: MTF-1 CG3743

(Bayraktaroglu, 2000.8.7)

Source for merge of: MTF-1 anon-WO0118547.411

(FlyBase, 1996-)

Additional comments

Source for merge of MTF-1 anon-WO0118547.411 was sequence comparison ( date:051113 ).

(FlyBase, 1996-)

Nomenclature History

Source for database identify of

Nomenclature comments

Etymology

Synonyms and Secondary IDs (15)

Reported As

Symbol Synonym

CG3743

(Hosono et al., 2015, Ni et al., 2010.12.1, Keleman et al., 2009.8.5)

MTF-1

(Hanson et al., 2025, Shin et al., 2025, Victor Atoki et al., 2025, Wang et al., 2025, Everman and Macdonald, 2024, Yu et al., 2023, Burke, 2022, Ding et al., 2022, Missirlis, 2021, Slobodian et al., 2021, Romine et al., 2019, Villanueva-Cañas et al., 2019, Calap-Quintana et al., 2018, Crofton et al., 2018, Monnier et al., 2018, Transgenic RNAi Project members, 2017-, Brewer-Jensen et al., 2016, Sarov et al., 2016, Soriano et al., 2016, Stepien et al., 2016, Lenartowicz et al., 2015, model organism Encyclopedia of Regulatory Network (modERN) Project, 2015-, Schertel et al., 2015, Luck et al., 2014, Montgomery et al., 2014, Cole et al., 2012.7.6, Günther et al., 2012, Marr et al., 2012, Sims et al., 2012, Atanesyan et al., 2011, Hua et al., 2011, Lindquist et al., 2011, Saini et al., 2011, Bahadorani et al., 2010, Bahadorani et al., 2010, Gutiérrez et al., 2010, Balamurugan et al., 2009, Hua et al., 2008, Liu and Lehmann, 2008, Zhou et al., 2008, Egli et al., 2006, Egli et al., 2006, Marr et al., 2006, Yepiskoposyan et al., 2006, Rehwinkel et al., 2005, Zhang et al., 2001)

Mtf-1

(Li et al., 2024, Burke et al., 2008)

anon-WO0118547.411

dMTF-1

(Fasae and Abolaji, 2022, Xiao, 2022, Marr et al., 2012, Chen et al., 2008, Vardanyan et al., 2008, Balamurugan et al., 2007, Egli et al., 2006, Selvaraj et al., 2005, Yepiskoposyan et al., 2005, Balamurugan et al., 2004, Yepiskoposyan et al., 2003)

mtf-1

(Selvaraj, 2003)

Name Synonyms

MTF-1

Metal response element-binding Transcription Factor-1

Metal-responsive transcription factor 1

(Vardanyan et al., 2008)

metal response element binding Transcription Factor 1

(Balamurugan et al., 2009)

metal response element binding transcription factor

(Hua et al., 2011)

metal response element-binding transcription factor-1

(Marr et al., 2006)

metal responsive transcription factor

(Hua et al., 2011, Bahadorani et al., 2010)

metal responsive transcription factor 1

(Balamurugan et al., 2009)

metal-regulatory transcription factor 1

(Atanesyan et al., 2011)

metal-responsive transcription factor-1

(Bahadorani et al., 2010)

Secondary FlyBase IDs

FBgn0035991
FBgn0062029

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 65 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

39089

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

A8JNP3

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

A8JNP3

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0040305

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

39089/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0040305

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0040305

FlyMine - An integrated database for Drosophila genomics

FBgn0040305

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG3743

MARRVEL_MODEL - MARRVEL (model organism gene)

39089

Linkouts

BioGRID - A database of protein and genetic interactions.

64482

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0040305

DroID - A comprehensive database of gene and protein interactions.

FBgn0040305

DRSC - Results frm RNAi screens

FBgn0040305

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0040305

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

DMEL:FBGN0040305

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG3743

Flygut - An atlas of the Drosophila adult midgut

FBgn0040305

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC013432

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

MIST (genetic) - An integrated Molecular Interaction Database

39089

MIST (protein-protein) - An integrated Molecular Interaction Database

39089

References (109)

The following external sites may use different assemblies or annotations than FlyBase.

-0.95

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