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General Information
Symbol
Dmel\ago
Species
D. melanogaster
Name
archipelago
Annotation Symbol
CG15010
Feature Type
FlyBase ID
FBgn0041171
Gene Model Status
Stock Availability
Gene Summary
archipelago (ago) encodes a protein that is the founding member of the Ago/Fbxw7 family of tumor suppressor genes and the substrate adaptor component of the SCF-Ago ubiquitin ligase. The product of ago has various substrates including the bHLH transcription factors encoded by Myc, trh, and sima, and the G1 cell cycle kinase component encoded by CycE. The product of ago presents tissue specific roles in cell proliferation, survival, and hypoxia-induced gene expression. [Date last reviewed: 2019-03-07] (FlyBase Gene Snapshot)
Key Links
Genomic Location
Cytogenetic map
Sequence location
Recombination map
3-10
RefSeq locus
NT_037436 REGION:4246343..4254306
Sequence
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
Function
GO Summary Ribbons
Gene Ontology (GO) Annotations (18 terms)
Molecular Function (3 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
inferred from physical interaction with FLYBASE:mi; FB:FBgn0261786
inferred from physical interaction with UniProtKB:Q9VC56
(assigned by UniProt )
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
traceable author statement
Biological Process (14 terms)
Terms Based on Experimental Evidence (11 terms)
CV Term
Evidence
References
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from genetic interaction with FLYBASE:Vhl; FB:FBgn0041174
inferred from high throughput mutant phenotype
Terms Based on Predictions or Assertions (3 terms)
CV Term
Evidence
References
traceable author statement
traceable author statement
Cellular Component (1 term)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
inferred from sequence or structural similarity with HGNC:16712
Gene Group (FlyBase)
Protein Family (UniProt)
-
Summaries
Gene Snapshot
archipelago (ago) encodes a protein that is the founding member of the Ago/Fbxw7 family of tumor suppressor genes and the substrate adaptor component of the SCF-Ago ubiquitin ligase. The product of ago has various substrates including the bHLH transcription factors encoded by Myc, trh, and sima, and the G1 cell cycle kinase component encoded by CycE. The product of ago presents tissue specific roles in cell proliferation, survival, and hypoxia-induced gene expression. [Date last reviewed: 2019-03-07]
Gene Group (FlyBase)
F BOX AND WD DOMAIN PROTEINS -
F box and WD domain (FBXW) proteins are F box proteins which contain WD40 repeats. F box proteins are the variable substrate adapters for Skp, Cullin, F-box (SCF) E3 ubiquitin ligase complexes. (Adapted from FBrf0225076).
Protein Function (UniProtKB)
Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins (By similarity). Probably recognizes and binds to phosphorylated target proteins (By similarity). In the wing and eye, negatively regulates cell growth and proliferation by mediating the degradation of Myc and cyclin E, respectively (PubMed:11565033, PubMed:15182669). Required for endocycles, but not mitosis in follicle cell epithelium (PubMed:15175253).
(UniProt, Q9VZF4)
Summary (Interactive Fly)
Gene Model and Products
Number of Transcripts
3
Number of Unique Polypeptides
1

Please see the JBrowse view of Dmel\ago for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Structure New Section
Protein 3D structure   (Predicted by AlphaFold)   (AlphaFold entry Q9VZF4)

If you don't see the viewer to the right, refresh your browser.
Model Confidence:
  • Very high (pLDDT > 90)
  • Confident (90 > pLDDT > 70)
  • Low (70 > pLDDT > 50)
  • Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Annotated transcripts do not represent all supported alternative splices within 5' UTR.

Gene model reviewed during 5.46

Sequence Ontology: Class of Gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
FBtr0073247
5223
1326
FBtr0073246
5268
1326
FBtr0073245
5332
1326
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
FBpp0073103
141.4
1326
4.90
FBpp0073102
141.4
1326
4.90
FBpp0073101
141.4
1326
4.90
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

1326 aa isoforms: ago-PA, ago-PB, ago-PC
Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Subunit Structure (UniProtKB)

Part of a SCF E3 ubiquitin-protein ligase complex. Interacts with Myc and puf (PubMed:15182669, PubMed:24173801). Interacts with CycE (PubMed:11565033).

(UniProt, Q9VZF4)
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\ago using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data

The ventrolateral body wall muscle 12 (VLM12) expresses endogenous, nuclear ago protein.

A strong pulse of nuclear ago protein is seen in the area of the morphogenetic furrow (MF) and are subsequently refined into a restricted pattern in the first few rows of cells posterior to the MF. ago becomes restricted to a single cell nucleus that occupies an anterior location within the nascent elav-positive cluster.

ago is widely expressed during most of embryogenesis and is concentrated in the nervous system at later stages.

Marker for
 
Subcellular Localization
CV Term
Evidence
References
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\ago in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 26 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 14 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of ago
Transgenic constructs containing regulatory region of ago
Aberrations (Deficiencies and Duplications) ( 1 )
Inferred from experimentation ( 1 )
Gene disrupted in
Inferred from location ( 0 )
Alleles Representing Disease-Implicated Variants
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
Sterility
Allele
Other Phenotypes
Allele
Phenotype manifest in
Allele
follicle cell & nucleus | somatic clone
nurse cell & nucleus | germ-line clone
Orthologs
Human Orthologs (via DIOPT v8.0)
Homo sapiens (Human) (16)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
8 of 15
Yes
Yes
2 of 15
No
No
1  
2 of 15
No
No
1  
1 of 15
No
Yes
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
Yes
Model Organism Orthologs (via DIOPT v8.0)
Mus musculus (laboratory mouse) (14)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
8 of 15
Yes
Yes
2 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
No
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
No
Rattus norvegicus (Norway rat) (10)
7 of 13
Yes
Yes
1 of 13
No
No
1 of 13
No
Yes
1 of 13
No
Yes
1 of 13
No
Yes
1 of 13
No
Yes
1 of 13
No
Yes
1 of 13
No
No
1 of 13
No
Yes
1 of 13
No
Yes
Xenopus tropicalis (Western clawed frog) (27)
7 of 12
Yes
Yes
4 of 12
No
Yes
2 of 12
No
No
2 of 12
No
Yes
2 of 12
No
Yes
2 of 12
No
Yes
2 of 12
No
Yes
2 of 12
No
Yes
2 of 12
No
Yes
2 of 12
No
Yes
2 of 12
No
Yes
2 of 12
No
Yes
2 of 12
No
Yes
2 of 12
No
Yes
1 of 12
No
No
1 of 12
No
Yes
1 of 12
No
Yes
1 of 12
No
Yes
1 of 12
No
Yes
1 of 12
No
Yes
1 of 12
No
Yes
1 of 12
No
Yes
1 of 12
No
Yes
1 of 12
No
Yes
1 of 12
No
Yes
1 of 12
No
Yes
1 of 12
No
Yes
Danio rerio (Zebrafish) (12)
6 of 15
Yes
Yes
2 of 15
No
No
2 of 15
No
No
1 of 15
No
No
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
Yes
Caenorhabditis elegans (Nematode, roundworm) (4)
8 of 15
Yes
Yes
2 of 15
No
No
1 of 15
No
Yes
1 of 15
No
No
Arabidopsis thaliana (thale-cress) (3)
1 of 9
Yes
Yes
1 of 9
Yes
No
1 of 9
Yes
No
Saccharomyces cerevisiae (Brewer's yeast) (6)
6 of 15
Yes
Yes
4 of 15
No
Yes
3 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
Yes
Schizosaccharomyces pombe (Fission yeast) (6)
5 of 12
Yes
Yes
4 of 12
No
Yes
2 of 12
No
Yes
1 of 12
No
No
1 of 12
No
Yes
1 of 12
No
No
Other Organism Orthologs (via OrthoDB)
Paralogs
Paralogs (via DIOPT v8.0)
Drosophila melanogaster (Fruit fly) (4)
3 of 10
2 of 10
1 of 10
1 of 10
Human Disease Associations
FlyBase Human Disease Model Reports
Disease Model Summary Ribbon
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Allele
Disease
Evidence
References
Potential Models Based on Orthology ( 0 )
Human Ortholog
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)
Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
Homo sapiens (Human)
Gene name
Score
OMIM
OMIM Phenotype
DO term
Complementation?
Transgene?
Functional Complementation Data
Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
Interactions
Summary of Physical Interactions
Summary of Genetic Interactions
esyN Network Diagram
esyN Network Key:
Suppression
Enhancement

Please look at the allele data for full details of the genetic interactions
Starting gene(s)
Interaction type
Interacting gene(s)
Reference
Starting gene(s)
Interaction type
Interacting gene(s)
Reference
External Data
Subunit Structure (UniProtKB)
Part of a SCF E3 ubiquitin-protein ligase complex. Interacts with Myc and puf (PubMed:15182669, PubMed:24173801). Interacts with CycE (PubMed:11565033).
(UniProt, Q9VZF4 )
Linkouts
BioGRID - A database of protein and genetic interactions.
DroID - A comprehensive database of gene and protein interactions.
MIST (genetic) - An integrated Molecular Interaction Database
MIST (protein-protein) - An integrated Molecular Interaction Database
Pathways
Signaling Pathways (FlyBase)
Metabolic Pathways
External Data
Linkouts
KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.
Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
Genomic Location and Detailed Mapping Data
Chromosome (arm)
3L
Recombination map
3-10
Cytogenetic map
Sequence location
FlyBase Computed Cytological Location
Cytogenetic map
Evidence for location
64A11-64A12
Limits computationally determined from genome sequence between P{PZ}l(3)rG166rG166 and P{PZ}sinu06524
Experimentally Determined Cytological Location
Cytogenetic map
Notes
References
Experimentally Determined Recombination Data
Left of (cM)
Right of (cM)
Notes
Stocks and Reagents
Stocks (8)
Genomic Clones (18)
 

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (88)
 

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced
BDGP DGC clones
Other clones
Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

cDNA Clones, End Sequenced (ESTs)
RNAi and Array Information
Linkouts
DRSC - Results frm RNAi screens
GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
Antibody Information
Laboratory Generated Antibodies
 
Commercially Available Antibodies
 
Other Information
Relationship to Other Genes
Source for database identify of
Source for database merge of

Source for merge of: ago CG15010

Source for merge of: ago anon-WO0118547.345

Additional comments

Source for merge of ago anon-WO0118547.345 was sequence comparison ( date:051113 ).

Other Comments

ago acts an antagonist of the physiological response to hypoxia. ago protein physically associates with sima protein and restricts sima protein levels in vivo. Reducing ago activity in larval muscle cells elicits enhanced branching of nearby tracheal cells in normoxia.

dsRNA has been made from templates generated with primers directed against this gene.

Mutants in ago show increased tissue growth.

dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.

Area matching Drosophila EST AA392932 (inverted).

ago is involved in developmental regulation of CycE degradation.

Clones of mutant cells in the eye disc result in cell fate changes and overrepresentation of mutant cells in the eye.

Origin and Etymology
Discoverer
Etymology
Identification
External Crossreferences and Linkouts ( 64 )
Sequence Crossreferences
NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.
UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
Other crossreferences
AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.
Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
KEGG Genes - Molecular building blocks of life in the genomic space.
MARRVEL_MODEL - MARRVEL (model organism gene)
modMine - A data warehouse for the modENCODE project
SignaLink - A signaling pathway resource with multi-layered regulatory networks.
Linkouts
BioGRID - A database of protein and genetic interactions.
DroID - A comprehensive database of gene and protein interactions.
DRSC - Results frm RNAi screens
FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
FlyMine - An integrated database for Drosophila genomics
Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.
MIST (genetic) - An integrated Molecular Interaction Database
MIST (protein-protein) - An integrated Molecular Interaction Database
Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
Synonyms and Secondary IDs (11)
Reported As
Symbol Synonym
anon-WO0118547.345
Secondary FlyBase IDs
  • FBgn0035516
  • FBgn0062079
Datasets (8)
Study focus (0)
Experimental Role
Project
Project Type
Title
Study result (8)
Result
Result Type
Title
Clustering analysis of hemocytes from non-infested third instar larvae
Clustering analysis of hemocytes from wasp-infested third instar larvae
Clustering analysis of lymph gland cells from non-infested larvae at 72 h after egg-laying
Clustering analysis of lymph gland cells from non-infested larvae at 96 h after egg-laying
Clustering analysis of lymph gland cells from non-infested larvae at 120 h after egg-laying
Clustering analysis of lymph gland cells from wasp-infested larvae at 96 h after egg-laying
Clustering analysis of circulating hemocytes from non-infested larvae at 96 h after egg-laying
Clustering analysis of circulating hemocytes from non-infested larvae at 120 h after egg-laying
References (134)