FB2021_01, released Feb 18, 2021

Gene: Dmel\ago

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General Information
Symbol
Dmel\ago
Species
D. melanogaster
Name
archipelago
Annotation Symbol
CG15010
Feature Type
protein_coding_gene
FlyBase ID
FBgn0041171
Gene Model Status
Current
Stock Availability
8 publicly available
Gene Snapshot
archipelago (ago) encodes a protein that is the founding member of the Ago/Fbxw7 family of tumor suppressor genes and the substrate adaptor component of the SCF-Ago ubiquitin ligase. The product of ago has various substrates including the bHLH transcription factors encoded by Myc, trh, and sima, and the G1 cell cycle kinase component encoded by CycE. The product of ago presents tissue specific roles in cell proliferation, survival, and hypoxia-induced gene expression. [Date last reviewed: 2019-03-07]
Other Summaries
Key Links
Genomic Location
Cytogenetic map
64A11-64A12
Sequence location
3L:4,246,343..4,254,306 [-]
Recombination map

3-10

RefSeq locus
NT_037436 REGION:4246343..4254306
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
GO Summary Ribbons
Gene Ontology (GO) Annotations (18 terms)
Molecular Function (3 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
enables protein binding
inferred from physical interaction with FLYBASE:mi; FB:FBgn0261786
(Szuplewski et al., 2009)
enables ubiquitin-specific protease binding
inferred from physical interaction with UniProtKB:Q9VC56
(assigned by UniProt )
(Li et al., 2013)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
enables cyclin binding
traceable author statement
(Lee and Orr-Weaver, 2003)
Biological Process (14 terms)
Terms Based on Experimental Evidence (11 terms)
CV Term
Evidence
References
involved_in branch fusion, open tracheal system
inferred from mutant phenotype
(Mortimer and Moberg, 2007)
involved_in cellular response to hypoxia
inferred from mutant phenotype
(Mortimer and Moberg, 2009)
involved_in DNA endoreduplication
inferred from mutant phenotype
(Shcherbata et al., 2004)
involved_in negative regulation of cellular response to hypoxia
inferred from mutant phenotype
(Mortimer and Moberg, 2013)
involved_in negative regulation of glial cell proliferation
inferred from genetic interaction with FLYBASE:slmb; FB:FBgn0283468
(Ho et al., 2009)
involved_in negative regulation of growth
inferred from mutant phenotype
(Moberg et al., 2004)
involved_in negative regulation of imaginal disc growth
inferred from mutant phenotype
(Gerlach et al., 2018)
involved_in regulation of autophagy of mitochondrion
inferred from mutant phenotype
(Ivatt et al., 2014)
involved_in regulation of mitotic nuclear division
inferred from mutant phenotype
(Moberg et al., 2001)
involved_in trachea development
inferred from mutant phenotype
(Mortimer and Moberg, 2009)
inferred from genetic interaction with FLYBASE:Vhl; FB:FBgn0041174
(Mortimer and Moberg, 2009)
involved_in ventral cord development
inferred from high throughput mutant phenotype
(Bivik et al., 2015)
Terms Based on Predictions or Assertions (3 terms)
CV Term
Evidence
References
involved_in regulation of exit from mitosis
traceable author statement
(Lee and Orr-Weaver, 2003)
involved_in regulation of proteolysis
traceable author statement
(Lee and Orr-Weaver, 2003)
involved_in SCF-dependent proteasomal ubiquitin-dependent protein catabolic process
inferred from sequence or structural similarity with HGNC:16712
(FlyBase Curators, 2008--)
Cellular Component (1 term)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
part_of SCF ubiquitin ligase complex
inferred from sequence or structural similarity with HGNC:16712
(FlyBase Curators, 2008--)
Gene Group (FlyBase)
Protein Family (UniProt)
-
Protein Signatures (InterPro)
Summaries
Gene Group (FlyBase)
F BOX AND WD DOMAIN PROTEINS -
F box and WD domain (FBXW) proteins are F box proteins which contain WD40 repeats. F box proteins are the variable substrate adapters for Skp, Cullin, F-box (SCF) E3 ubiquitin ligase complexes. (Adapted from FBrf0225076).
Protein Function (UniProtKB)
Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins (By similarity). Probably recognizes and binds to phosphorylated target proteins (By similarity). In the wing and eye, negatively regulates cell growth and proliferation by mediating the degradation of Myc and cyclin E, respectively (PubMed:11565033, PubMed:15182669). Required for endocycles, but not mitosis in follicle cell epithelium (PubMed:15175253).
(UniProt, Q9VZF4)
Summary (Interactive Fly)
Gene Model and Products
Number of Transcripts
3
Number of Unique Polypeptides
1

Please see the JBrowse view of Dmel\ago for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Annotated transcripts do not represent all supported alternative splices within 5' UTR.

Gene model reviewed during 5.46

Sequence Ontology: Class of Gene
nuclear_gene
protein_coding_gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
ago-RA
FBtr0073247
NM_168072
5223
1326
ago-RB
FBtr0073246
NM_168073
5268
1326
ago-RC
FBtr0073245
NM_079198
5332
1326
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
ago-PA
FBpp0073103
141.4
1326
4.90
NP_728964
AAF47869
ago-PB
FBpp0073102
141.4
1326
4.90
NP_728965
AAG22247
ago-PC
FBpp0073101
141.4
1326
4.90
NP_523922
AAG22246
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

1326 aa isoforms: ago-PA, ago-PB, ago-PC
Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Subunit Structure (UniProtKB)

Part of a SCF E3 ubiquitin-protein ligase complex. Interacts with Myc and puf (PubMed:15182669, PubMed:24173801). Interacts with CycE (PubMed:11565033).

(UniProt, Q9VZF4)
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\ago using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 3 -- 13
organism | ubiquitous

Comment: but not in pole cells

(Mortimer and Moberg, 2007)
embryonic stage 15
embryonic/larval central nervous system
(Mortimer and Moberg, 2007)
larval stage
morphogenetic furrow
(Nicholson et al., 2011)
third instar larval stage
A1-7 ventral longitudinal muscle 1
(Mortimer and Moberg, 2013)
Additional Descriptive Data

The ventrolateral body wall muscle 12 (VLM12) expresses endogenous, nuclear ago protein.

(Mortimer and Moberg, 2013)

A strong pulse of nuclear ago protein is seen in the area of the morphogenetic furrow (MF) and are subsequently refined into a restricted pattern in the first few rows of cells posterior to the MF. ago becomes restricted to a single cell nucleus that occupies an anterior location within the nascent elav-positive cluster.

(Nicholson et al., 2011)

ago is widely expressed during most of embryogenesis and is concentrated in the nervous system at later stages.

(Mortimer and Moberg, 2007)
Marker for
 
Subcellular Localization
CV Term
Evidence
References
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\ago in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
FLIGHT - Cell culture data for RNAi and other high-throughput technologies
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 26 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 13 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of ago
Transgenic constructs containing regulatory region of ago
Aberrations (Deficiencies and Duplications) ( 1 )
Inferred from experimentation
Gene disrupted in
Df(3L)Exel9000
(Ryder, 2004.4.26)
Inferred from location
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
lethal
lethal (with ago1)
lethal (with ago3)
lethal (with agoΔ3-7)
lethal - all die before end of first instar larval stage (with ago1)
lethal - all die before end of first instar larval stage (with ago3)
lethal - all die before end of first instar larval stage (with Df(3L)Exel9000)
Other Phenotypes
Allele
abnormal cell number | germline clone | oogenesis
abnormal mitotic cell cycle | somatic clone
abnormal neuroanatomy
abnormal neuroanatomy | somatic clone | third instar larval stage
abnormal oxidative stress response (with agoEP1135)
abnormal oxidative stress response (with agoΔ3-7)
decreased body size, with Scer\GAL4en-e16E
decreased cell number | adult stage | somatic clone
decreased cell size, with Scer\GAL4en-e16E
flightless, with Scer\GAL4Mef2.PR
increased cell number | somatic clone
increased cell size, with Scer\GAL4en.PU
increased occurrence of cell division | larval stage | somatic clone
increased occurrence of cell division | pupal stage | somatic clone
increased occurrence of cell division | somatic clone
some die during first instar larval stage (with ago1)
some die during first instar larval stage (with ago3)
some die during first instar larval stage (with Df(3L)Exel9000)
some die during second instar larval stage
some die during third instar larval stage
visible, with Scer\GAL4Bx-MS1096
visible, with Scer\GAL4salm.EPv
visible | recessive | somatic clone
Phenotype manifest in
Allele
apterous-expressing neuron of the lateral cluster of the thorax
apterous-expressing neuron of the lateral cluster of the thorax Ap4
embryonic/larval dorsal branch (with ago1)
embryonic/larval dorsal branch (with ago3)
embryonic/larval dorsal trunk, with Scer\GAL4btl.PS
embryonic/larval dorsal trunk (with ago1)
embryonic/larval dorsal trunk (with ago3)
embryonic/larval ganglionic branch (with ago1)
embryonic/larval ganglionic branch (with ago3)
embryonic/larval ganglionic branch (with agoΔ3-7)
embryonic/larval lateral trunk (with ago1)
embryonic/larval lateral trunk (with ago3)
embryonic/larval trachea | conditional
embryonic/larval tracheal system, with Scer\GAL4btl.PS
embryonic/larval tracheal system (with ago1)
embryonic/larval tracheal system (with ago3)
eye | somatic clone
eye disc | pupal stage | somatic clone
follicle cell & nucleus | somatic clone
interommatidial bristle | ectopic | somatic clone
lateral F branch | cell non-autonomous, with Scer\GAL4tey-5053A
lateral G branch (with ago1)
lateral G branch (with agoΔ3-7)
lateral H branch (with ago1)
lateral H branch (with ago3)
lateral H branch (with agoΔ3-7)
lateral H branch | cell non-autonomous, with Scer\GAL4tey-5053A
mitotic cell cycle | somatic clone
nurse cell & nucleus | germ-line clone
nurse cell | germline clone
RP2 motor neuron | ectopic
RP2 motor neuron | ectopic (with Df(3L)GN50)
secondary pigment cell | ectopic | somatic clone
tertiary pigment cell | ectopic | somatic clone
tracheal fusion cell (with ago1)
tracheal fusion cell (with ago3)
tracheal lumen (with ago1)
tracheal lumen (with ago3)
tracheal primordium (with ago1)
tracheal primordium (with ago3)
tracheole (with ago1)
tracheole (with agoΔ3-7)
tracheole | cell non-autonomous, with Scer\GAL4tey-5053A
tracheole | increased number (with ago1)
tracheole | increased number (with ago3)
tracheole | increased number (with agoΔ3-7)
tracheole | increased number | heat sensitive, with Scer\GAL4hs.PB
wing, with Scer\GAL4Bx-MS1096
wing, with Scer\GAL4salm.EPv
wing | posterior, with Scer\GAL4en.PU
wing blade posterior compartment, with Scer\GAL4en-e16E
wing disc | larval stage | somatic clone
wing disc | somatic clone
wing vein, with Scer\GAL4en.PU
Orthologs
Downloads
Download All DIOPT Orthologs
Download All OrthoDB Orthologs
Human Orthologs (via DIOPT v8.0)
Homo sapiens (Human) (16)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Hsap\FBXW7
8 of 15
Yes
Yes
Hsap\BTRC
2 of 15
No
No
1  
Hsap\FBXW11
2 of 15
No
No
Hsap\CDRT1
1 of 15
No
Yes
Hsap\DAW1
1 of 15
No
No
Hsap\ECT2L
1 of 15
No
No
Hsap\EFCAB8
1 of 15
No
No
Hsap\FBXO16
1 of 15
No
Yes
Hsap\FBXO24
1 of 15
No
Yes
Hsap\FBXW2
1 of 15
No
Yes
Hsap\FBXW8
1 of 15
No
Yes
Hsap\FBXW9
1 of 15
No
Yes
Hsap\FBXW10
1 of 15
No
Yes
Hsap\TRAF7
1 of 15
No
Yes
Hsap\TRIM16L
1 of 15
No
Yes
Hsap\WDR86
1 of 15
No
Yes
Model Organism Orthologs (via DIOPT v8.0)
Mus musculus (laboratory mouse) (14)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Mmus\Fbxw7
8 of 15
Yes
Yes
Mmus\Btrc
2 of 15
No
No
Mmus\Daw1
1 of 15
No
No
Mmus\Ect2l
1 of 15
No
No
Mmus\Fbxo16
1 of 15
No
Yes
Mmus\Fbxo24
1 of 15
No
Yes
Mmus\Fbxw2
1 of 15
No
Yes
Mmus\Fbxw8
1 of 15
No
Yes
Mmus\Fbxw9
1 of 15
No
Yes
Mmus\Fbxw10
1 of 15
No
Yes
Mmus\Fbxw11
1 of 15
No
No
Mmus\Traf7
1 of 15
No
Yes
Mmus\Wdr86
1 of 15
No
Yes
Mmus\Wdr95
1 of 15
No
No
Rattus norvegicus (Norway rat) (10)
Rnor\Fbxw7
7 of 13
Yes
Yes
Rnor\Btrc
1 of 13
No
No
Rnor\Fbxo16
1 of 13
No
Yes
Rnor\Fbxo24
1 of 13
No
Yes
Rnor\Fbxw2
1 of 13
No
Yes
Rnor\Fbxw8
1 of 13
No
Yes
Rnor\Fbxw9
1 of 13
No
Yes
Rnor\Fbxw11
1 of 13
No
No
Rnor\Traf7
1 of 13
No
Yes
Rnor\Wdr95
1 of 13
No
Yes
Xenopus tropicalis (Western clawed frog) (27)
Xtro\fbxw7
7 of 12
Yes
Yes
Xtro\LOC100488030
4 of 12
No
Yes
Xtro\btrc
2 of 12
No
No
Xtro\LOC100487231
2 of 12
No
Yes
Xtro\LOC100491484
2 of 12
No
Yes
Xtro\LOC100492083
2 of 12
No
Yes
Xtro\LOC100492569
2 of 12
No
Yes
Xtro\LOC100492746
2 of 12
No
Yes
Xtro\LOC101733582
2 of 12
No
Yes
Xtro\LOC101734025
2 of 12
No
Yes
Xtro\LOC101734139
2 of 12
No
Yes
Xtro\LOC101734255
2 of 12
No
Yes
Xtro\LOC108644489
2 of 12
No
Yes
Xtro\LOC108644802
2 of 12
No
Yes
Xtro\daw1
1 of 12
No
No
Xtro\efcab8
1 of 12
No
Yes
Xtro\fbxo16
1 of 12
No
Yes
Xtro\fbxo24
1 of 12
No
Yes
Xtro\fbxw2
1 of 12
No
Yes
Xtro\fbxw8
1 of 12
No
Yes
Xtro\fbxw9
1 of 12
No
Yes
Xtro\LOC733932
1 of 12
No
Yes
Xtro\LOC100158595
1 of 12
No
Yes
Xtro\LOC100491574
1 of 12
No
Yes
Xtro\LOC100496047
1 of 12
No
Yes
Xtro\LOC108644804
1 of 12
No
Yes
Xtro\traf7
1 of 12
No
Yes
Danio rerio (Zebrafish) (12)
Drer\fbxw7
6 of 15
Yes
Yes
Drer\fbxw11a
2 of 15
No
No
Drer\fbxw11b
2 of 15
No
No
Drer\daw1
1 of 15
No
No
Drer\ect2l
1 of 15
No
Yes
Drer\fbxo16
1 of 15
No
Yes
Drer\fbxw2
1 of 15
No
Yes
Drer\fbxw8
1 of 15
No
Yes
Drer\fbxw9
1 of 15
No
Yes
Drer\fbxw10
1 of 15
No
Yes
Drer\si:ch211-154o6.3
1 of 15
No
No
Drer\traf7
1 of 15
No
Yes
Caenorhabditis elegans (Nematode, roundworm) (4)
Cele\sel-10
8 of 15
Yes
Yes
Cele\lin-23
2 of 15
No
No
Cele\mec-15
1 of 15
No
Yes
Cele\scp-1
1 of 15
No
No
Arabidopsis thaliana (thale-cress) (3)
Atha\AT3G52030
1 of 9
Yes
Yes
Atha\WDR5a
1 of 9
Yes
No
Atha\WDR5b
1 of 9
Yes
No
Saccharomyces cerevisiae (Brewer's yeast) (6)
Scer\CDC4
6 of 15
Yes
Yes
Scer\RRT13
4 of 15
No
Yes
Scer\MET30
3 of 15
No
Yes
Scer\CAF4
1 of 15
No
Yes
Scer\MDV1
1 of 15
No
Yes
Scer\UFO1
1 of 15
No
Yes
Schizosaccharomyces pombe (Fission yeast) (6)
Spom\pop2
5 of 12
Yes
Yes
Spom\pop1
4 of 12
No
Yes
Spom\pof1
2 of 12
No
Yes
Spom\pof11
1 of 12
No
No
Spom\raf1
1 of 12
No
Yes
Spom\swd3
1 of 12
No
No
Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG091902NN )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila suzukii
Spotted wing Drosophila
Dsuz\DS10_00010058
Drosophila simulans
Dsim\GD13273
Drosophila sechellia
Dsec\GM13994
Drosophila erecta
Dere\GG14203
Drosophila yakuba
Dyak\GE20631
Drosophila ananassae
Dana\GF23871
Drosophila pseudoobscura pseudoobscura
Dpse\GA13429
Drosophila persimilis
Dper\GL12727
Drosophila willistoni
Dwil\GK10570
Drosophila virilis
Dvir\GJ12563
Drosophila mojavensis
Dmoj\GI16816
Drosophila grimshawi
Dgri\anon-2
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091500R5 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Mdoa\17020453
Glossina morsitans
Tsetse fly
Gmor\GMOY001726
Lucilia cuprina
Australian sheep blowfly
Lcup\FF38_02600
Mayetiola destructor
Hessian fly
Mdes\Mdes000320
Aedes aegypti
Yellow fever mosquito
Aaeg\AAEL015522
Aedes aegypti
Yellow fever mosquito
Aaeg\AAEL011215
Anopheles darlingi
American malaria mosquito
Adar\ADAC010478
Anopheles gambiae
Malaria mosquito
Agam\AGAP005359
Culex quinquefasciatus
Southern house mosquito
Cqui\CPIJ012122
Culex quinquefasciatus
Southern house mosquito
Cqui\CPIJ011984
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W026W )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Bmor\BGIBMGA012354
Bombyx mori
Silkmoth
Bmor\BGIBMGA012355
Danaus plexippus
Monarch butterfly
Dple\DPOGS208365-PA
Heliconius melpomene
Postman butterfly
Hmel\HMEL008790
Apis florea
Little honeybee
Aflo\2446442708
Apis mellifera
Western honey bee
Amel\GB45868
Bombus impatiens
Common eastern bumble bee
Bimp\BIMP22449
Bombus terrestris
Buff-tailed bumblebee
Bter\15214092
Linepithema humile
Argentine ant
Lhum\LH11801
Megachile rotundata
Alfalfa leafcutting bee
Mrot\MROTU:00125d
Nasonia vitripennis
Parasitic wasp
Nvit\Nasvi2EG000076
Dendroctonus ponderosae
Mountain pine beetle
Dpod\YQE_02181
Tribolium castaneum
Red flour beetle
Tcas\ago
Pediculus humanus
Human body louse
Phum\PHUM539800
Rhodnius prolixus
Kissing bug
Rpro\RPRC014551
Cimex lectularius
Bed bug
Clec\CLEC000335
Acyrthosiphon pisum
Pea aphid
Apim\ACYPI004580
Acyrthosiphon pisum
Pea aphid
Apim\ACYPI007103
Zootermopsis nevadensis
Nevada dampwood termite
Znev\Znev_06051
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X0247 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strigamia maritima
European centipede
Smar\SMAR002788
Ixodes scapularis
Black-legged tick
Isca\ISCW017325
Stegodyphus mimosarum
African social velvet spider
Smim\KFM81030.1
Stegodyphus mimosarum
African social velvet spider
Smim\KFM81029.1
Tetranychus urticae
Two-spotted spider mite
Turt\tetur26g01760
Tetranychus urticae
Two-spotted spider mite
Turt\tetur04g00450
Tetranychus urticae
Two-spotted spider mite
Turt\tetur110g00030
Daphnia pulex
Water flea
Dpux\DAPPUDRAFT_50336
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G04EW )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Fbw7
Ciona intestinalis
Vase tunicate
Cint\LOC100183472
Ciona intestinalis
Vase tunicate
Cint\ENSCING00000001520
Gallus gallus
Domestic chicken
Ggal\FBXW7
Gallus gallus
Domestic chicken
Ggal\LOC419074
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v8.0)
Drosophila melanogaster (Fruit fly) (4)
Gene Symbol
Score
Source
Compara
eggNOG
Homologene
Inparanoid
Isobase
OrthoDB
OrthoFinder
orthoMCL
Panther
RoundUp
Alignment
slmb
3 of 10
WDY
2 of 10
CG7568
1 of 10
wds
1 of 10
Human Disease Associations
FlyBase Human Disease Model Reports
Disease Model Summary Ribbon
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Allele
Disease
Evidence
References
Potential Models Based on Orthology ( 0 )
Human Ortholog
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)
Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
Homo sapiens (Human)
Gene name
Score
OMIM
OMIM Phenotype
DO term
Complementation?
Transgene?
FBXW7; F-box and WD repeat domain containing 7
8 of 15
606278
      Functional Complementation Data
      Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
      Interactions
      Summary of Physical Interactions
      Summary of Genetic Interactions
      esyN Network Diagram
      esyN Network Key:
      Suppression
      Enhancement
      View in Interactions Browser
      Please look at the allele data for full details of the genetic interactions
      Starting gene(s)
      Interaction type
      Interacting gene(s)
      Reference
      ago
      enhanceable
      awd
      (Mortimer and Moberg, 2007)
      ago
      suppressible
      bnl
      (Mortimer and Moberg, 2013)
      ago
      suppressible
      btl
      (Mortimer and Moberg, 2007)
      ago|slmb
      suppressible
      gcm
      (Ho et al., 2009)
      ago|Myc
      suppressible
      puf
      (Li et al., 2013)
      ago|Myc
      enhanceable
      puf
      (Li et al., 2013)
      ago
      suppressible
      sima
      (Mortimer and Moberg, 2013)
      ago
      suppressible
      trh
      (Mortimer and Moberg, 2007)
      ago
      enhanceable
      Vhl
      (Mortimer and Moberg, 2013)
      ago
      suppressible
      Vhl
      (Mortimer and Moberg, 2013)
      Starting gene(s)
      Interaction type
      Interacting gene(s)
      Reference
      btl::Egfr
      enhanceable
      ago
      (Zhu et al., 2005)
      btl|stumps
      suppressible
      ago
      (Zhu et al., 2005)
      caup
      enhanceable
      ago
      (Barrios et al., 2015)
      COX5A
      suppressible
      ago
      (Mandal et al., 2010)
      CycE
      suppressible
      ago
      (Brumby et al., 2004, Moberg et al., 2001)
      Myc
      suppressible
      ago
      (Li et al., 2013, Moberg et al., 2004)
      Myc
      enhanceable
      ago
      (Li et al., 2013, Secombe et al., 2007)
      numb
      suppressible
      ago
      (Ouyang et al., 2011)
      p53
      suppressible
      ago
      (Ouyang et al., 2011)
      puf
      enhanceable
      ago
      (Li et al., 2013)
      Pvr
      enhanceable
      ago
      (Zhu et al., 2005)
      Ras85D
      enhanceable
      ago
      (Friedman et al., 2011)
      Vhl
      enhanceable
      ago
      (Mortimer and Moberg, 2013, Mortimer and Moberg, 2009)
      Vhl
      suppressible
      ago
      (Mortimer and Moberg, 2013)
      External Data
      Subunit Structure (UniProtKB)
      Part of a SCF E3 ubiquitin-protein ligase complex. Interacts with Myc and puf (PubMed:15182669, PubMed:24173801). Interacts with CycE (PubMed:11565033).
      (UniProt, Q9VZF4 )
      Linkouts
      BioGRID - A database of protein and genetic interactions.
      DroID - A comprehensive database of gene and protein interactions.
      InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
      MIST (genetic) - An integrated Molecular Interaction Database
      MIST (protein-protein) - An integrated Molecular Interaction Database
      Pathways
      Signaling Pathways (FlyBase)
      Metabolic Pathways
      External Data
      Linkouts
      KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.
      Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
      Genomic Location and Detailed Mapping Data
      Chromosome (arm)
      3L
      Recombination map

      3-10

      Cytogenetic map
      64A11-64A12
      Sequence location
      3L:4,246,343..4,254,306 [-]
      FlyBase Computed Cytological Location
      Cytogenetic map
      Evidence for location
      64A11-64A12
      Limits computationally determined from genome sequence between P{PZ}l(3)rG166rG166 and P{PZ}sinu06524
      Experimentally Determined Cytological Location
      Cytogenetic map
      Notes
      References
      Experimentally Determined Recombination Data
      Location

      3-10

      (FlyBase, 2016)

      3-9.2

      (Comeron et al., 2012)

      3-

      (Moberg and Hariharan, 2000)
      Left of (cM)
      Right of (cM)
      Notes
      Stocks and Reagents
      Stocks (8)
      Genomic Clones (18)
       

      Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

      cDNA Clones (88)
       

      Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

      cDNA clones, fully sequenced
      BDGP DGC clones
      Other clones
      Drosophila Genomics Resource Center cDNA clones

      For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

      cDNA Clones, End Sequenced (ESTs)
      BDGP DGC clones
      Other clones
      RNAi and Array Information
      Linkouts
      DRSC - Results frm RNAi screens
      GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
      Antibody Information
      Laboratory Generated Antibodies
       
      Commercially Available Antibodies
       
      Other Information
      Relationship to Other Genes
      Source for database identify of
      Source for database merge of

      Source for merge of: ago CG15010

      (Moberg et al., 2001)

      Source for merge of: ago anon-WO0118547.345

      (FlyBase, 1996-)
      Additional comments

      Source for merge of ago anon-WO0118547.345 was sequence comparison ( date:051113 ).

      (FlyBase, 1996-)
      Other Comments

      ago acts an antagonist of the physiological response to hypoxia. ago protein physically associates with sima protein and restricts sima protein levels in vivo. Reducing ago activity in larval muscle cells elicits enhanced branching of nearby tracheal cells in normoxia.

      (Mortimer and Moberg, 2013)

      dsRNA has been made from templates generated with primers directed against this gene.

      (Pilot et al., 2006)

      Mutants in ago show increased tissue growth.

      (Moberg et al., 2004)

      dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.

      (Kiger et al., 2003)

      Area matching Drosophila EST AA392932 (inverted).

      (Davies, 2001.3.15)

      ago is involved in developmental regulation of CycE degradation.

      (Moberg et al., 2001)

      Clones of mutant cells in the eye disc result in cell fate changes and overrepresentation of mutant cells in the eye.

      (Moberg and Hariharan, 2000)
      Origin and Etymology
      Discoverer
      Etymology
      Identification
      External Crossreferences and Linkouts ( 62 )
      Sequence Crossreferences
      NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
      GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
      GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
      RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
      UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
      Other crossreferences
      Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
      Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
      Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
      Flygut - An atlas of the Drosophila adult midgut
      GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
      iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
      InterPro - A database of protein families, domains and functional sites
      KEGG Genes - Molecular building blocks of life in the genomic space.
      modMine - A data warehouse for the modENCODE project
      SignaLink - A signaling pathway resource with multi-layered regulatory networks.
      Linkouts
      BioGRID - A database of protein and genetic interactions.
      DPiM - Drosophila Protein interaction map
      DroID - A comprehensive database of gene and protein interactions.
      DRSC - Results frm RNAi screens
      FLIGHT - Cell culture data for RNAi and other high-throughput technologies
      FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
      FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
      FlyMine - An integrated database for Drosophila genomics
      Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
      InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
      KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.
      MIST (genetic) - An integrated Molecular Interaction Database
      MIST (protein-protein) - An integrated Molecular Interaction Database
      Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
      Synonyms and Secondary IDs (11)
      Reported As
      Symbol Synonym
      Ago
      (Barbosa et al., 2021, Chen et al., 2019, Mirzoyan et al., 2019, Lee et al., 2017, Yadav et al., 2016, Barrios and Campuzano, 2015, Li et al., 2015, Wong et al., 2013, Lee et al., 2012, Roberts et al., 2012, Klusza and Deng, 2011, Cunha-Ferreira et al., 2009, Skaar et al., 2009, Shibutani et al., 2008, Galletti et al., 2007, Martin et al., 2007)
      CG15010
      (Meadows, 2018.10.31, Schertel et al., 2013, Roberts et al., 2012, Ni et al., 2011.7.19, Berger et al., 2008, Ni et al., 2008, Gandhi et al., 2007, Pilot et al., 2006, Stanyon et al., 2004, Das et al., 2002, Halfon et al., 2002, Koepp et al., 2001)
      DmFbw7
      (Koepp et al., 2001)
      FBXW3
      (Roberts et al., 2012)
      FBXW7
      (Ivatt et al., 2014)
      SCFAgo
      (Mortimer and Moberg, 2005)
      ago
      (Kuang et al., 2020, Thevenon et al., 2020, Bangi et al., 2019, Bivik Stadler et al., 2019, Deshpande et al., 2019, Meltzer et al., 2019, Zahoor et al., 2019, Bala Tannan et al., 2018, Eusebio et al., 2018, Salazar and Yamamoto, 2018, Li et al., 2017, Suisse et al., 2017, Barrios et al., 2015, Bivik et al., 2015, Ribeiro et al., 2014, Bandura et al., 2013, Li et al., 2013, Mortimer and Moberg, 2013, Schertel et al., 2013, Webber et al., 2013, Adamson and Lajeunesse, 2012, Japanese National Institute of Genetics, 2012.5.21, Roberts et al., 2012, Du et al., 2011, Friedman et al., 2011, Nicholson et al., 2011, Toku et al., 2011, Ho et al., 2009, Mortimer and Moberg, 2009, Nicholson et al., 2009, Rhiner et al., 2009, Szuplewski et al., 2009, Ho et al., 2008, Zhou et al., 2008, Magalhaes et al., 2007, Mortimer and Moberg, 2007, Mortimer and Moberg, 2007, Secombe et al., 2007, Tyler et al., 2007, Ho et al., 2006, Firth and Baker, 2005)
      anon-WO0118547.345
      cdc4
      (Brumby et al., 2004)
      Name Synonyms
      Archipelago
      (Barrios et al., 2015, Pawar et al., 2011, Herranz et al., 2010, Ho et al., 2009, Galletti et al., 2007, Mortimer and Moberg, 2007, Secombe et al., 2007)
      archipelago
      (Salazar and Yamamoto, 2018, Hsu and Drummond-Barbosa, 2017, Bandura et al., 2013, Li et al., 2013, Ouyang et al., 2011, Mandal et al., 2010, Mortimer and Moberg, 2009, Nicholson et al., 2009, Rhiner et al., 2009, Szuplewski et al., 2009, Berger et al., 2008, Mortimer and Moberg, 2007, Tountas and Fortini, 2007, Ho et al., 2006, Moberg and Hariharan, 2000)
      Secondary FlyBase IDs
      • FBgn0035516
      • FBgn0062079
      Datasets (0)
      Study focus (0)
      Experimental Role
      Project
      Project Type
      Title
      References (132)