FB2025_05 , released December 11, 2025
Gene: Dmel\cpx
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General Information
Symbol
Dmel\cpx
Species
D. melanogaster
Name
complexin
Annotation Symbol
CG32490
Feature Type
protein_coding_gene
FlyBase ID
FBgn0041605
Gene Model Status
Current
Stock Availability
54 publicly available
Gene Summary
complexin (cpx) encodes a presynaptic cytosolic protein that regulates SNARE complex assembly and fuction. It has both positive and negative roles in synaptic transmission, serving as the synaptic vesicle fusion clamp and as an activator of evoked release. [Date last reviewed: 2019-03-07] (FlyBase Gene Snapshot)
All Summaries
Also Known As

DmCpx

Key Links
Genomic Location
Cytogenetic map
82A1-82A3
Sequence location
Recombination map
3-47.1
RefSeq locus
NT_033777 REGION:4280221..4304604
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
Gene Ontology (GO) Annotations (19 terms)
Molecular Function (2 terms)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
enables SNARE binding
inferred from biological aspect of ancestor with PANTHER:PTN001047612
(GO Reference Genome Project, 2011-)
enables syntaxin binding
inferred from electronic annotation with InterPro:IPR008849
(InterPro Project Members, 2004-)
Biological Process (9 terms)
Terms Based on Experimental Evidence (7 terms)
CV Term
Evidence
References
involved_in modulation of chemical synaptic transmission
inferred from mutant phenotype
(Buhl et al., 2013, Cho et al., 2010)
involved_in negative regulation of neurotransmitter secretion
inferred from mutant phenotype
(Mahoney et al., 2016)
involved_in neuromuscular synaptic transmission
inferred from mutant phenotype
(Iyer et al., 2013)
involved_in regulation of neurotransmitter secretion
inferred from mutant phenotype
(Xue et al., 2009)
involved_in synaptic assembly at neuromuscular junction
inferred from mutant phenotype
(Huntwork and Littleton, 2007)
involved_in synaptic vesicle exocytosis
inferred from mutant phenotype
(Huntwork and Littleton, 2007)
involved_in synaptic vesicle tethering involved in synaptic vesicle exocytosis
inferred from mutant phenotype
(Scholz et al., 2019)
Terms Based on Predictions or Assertions (4 terms)
CV Term
Evidence
References
involved_in modulation of chemical synaptic transmission
inferred from biological aspect of ancestor with PANTHER:PTN000422952
(GO Reference Genome Project, 2011-)
involved_in neurotransmitter transport
inferred from electronic annotation with InterPro:IPR008849
(InterPro Project Members, 2004-)
involved_in regulation of synaptic vesicle fusion to presynaptic active zone membrane
inferred from biological aspect of ancestor with PANTHER:PTN000422952
(GO Reference Genome Project, 2011-)
involved_in synaptic vesicle exocytosis
inferred from sequence or structural similarity with UniProtKB:P63040
(Gene Ontology Curators, 2002-)
inferred from biological aspect of ancestor with PANTHER:PTN000422952
(GO Reference Genome Project, 2011-)
Cellular Component (8 terms)
Terms Based on Experimental Evidence (7 terms)
CV Term
Evidence
References
located_in cytosol
inferred from high throughput direct assay
(Lye et al., 2014)
located_in membrane
inferred from direct assay
(Iyer et al., 2013)
located_in neuromuscular junction
inferred from direct assay
(Lowe et al., 2014)
located_in presynaptic active zone
inferred from direct assay
(Iyer et al., 2013)
located_in synaptic vesicle membrane
inferred from mutant phenotype
(Scholz et al., 2019)
located_in terminal bouton
inferred from direct assay
(Buhl et al., 2013, Cho et al., 2010)
located_in type I terminal bouton
inferred from direct assay
(Mahoney et al., 2016)
Terms Based on Predictions or Assertions (3 terms)
CV Term
Evidence
References
located_in membrane
inferred from sequence or structural similarity with UniProtKB:Q8R1B5
(Gene Ontology Curators, 2002-)
part_of SNARE complex
inferred from biological aspect of ancestor with PANTHER:PTN001047612
(GO Reference Genome Project, 2011-)
is_active_in terminal bouton
inferred from biological aspect of ancestor with PANTHER:PTN000422952
(GO Reference Genome Project, 2011-)
Gene Group (FlyBase)
Protein Family (UniProt)
Belongs to the complexin/synaphin family. (Q8IPM8)
Protein Signatures (InterPro)
Summaries
Gene Snapshot
complexin (cpx) encodes a presynaptic cytosolic protein that regulates SNARE complex assembly and fuction. It has both positive and negative roles in synaptic transmission, serving as the synaptic vesicle fusion clamp and as an activator of evoked release. [Date last reviewed: 2019-03-07]
Protein Function (UniProtKB)
Positively regulates a late step in synaptic vesicle exocytosis.
(UniProt, Q8IPM8)
Summary (Interactive Fly)

SNARE-complex-binding proteins that regulate synaptic vesicle release and consequently neurotransmitter release

Gene Model and Products
Number of Transcripts
21
Number of Unique Polypeptides
10

Please see the JBrowse view of Dmel\cpx for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Structure
Protein 3D structure   (Predicted by AlphaFold)   (AlphaFold entry Q8IPM8)

If you don't see a structure in the viewer, refresh your browser.
Model Confidence:
  • Very high (pLDDT > 90)
  • Confident (90 > pLDDT > 70)
  • Low (70 > pLDDT > 50)
  • Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures
Crossreferences
Comments on Gene Model

Gene model reviewed during 5.53

Gene model reviewed during 5.43

Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.

Annotated transcripts do not represent all supported alternative splices within 5' UTR.

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.47

Tissue-specific extension of 3' UTRs observed during later stages (FBrf0218523, FBrf0219848); all variants may not be annotated

Gene model reviewed during 5.56

Gene model reviewed during 6.01

Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
cpx-RA
FBtr0078901
NM_164327
3541
142
cpx-RAA
FBtr0310614
NM_001104208
3803
125
cpx-RAB
FBtr0336482
NM_001275319
9682
157
cpx-RC
FBtr0078900
NM_164330
3650
139
cpx-RE
FBtr0078902
NM_080280
2888
138
cpx-RG
FBtr0078897
NM_164328
3628
142
cpx-RH
FBtr0078899
NM_164331
3930
142
cpx-RI
FBtr0078898
NM_164329
3577
142
cpx-RJ
FBtr0100499
NM_001014603
3233
83
cpx-RK
FBtr0100500
NM_001014602
3682
83
cpx-RL
FBtr0113337
NM_001104203
3485
128
cpx-RM
FBtr0113338
NM_001104204
3649
139
cpx-RO
FBtr0113340
NM_001104206
3576
139
cpx-RP
FBtr0113341
NM_001104207
3634
139
cpx-RS
FBtr0300789
NM_001170028
3655
146
cpx-RU
FBtr0301768
NM_001170029
5137
143
cpx-RV
FBtr0301769
NM_001170030
3538
142
cpx-RW
FBtr0301770
NM_001170031
3561
139
cpx-RX
FBtr0301771
NM_001170032
8078
142
cpx-RY
FBtr0301772
NM_001170033
3544
143
cpx-RZ
FBtr0310506
NM_001275318
2094
98
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
UniProt
RefSeq ID
GenBank
cpx-PA
FBpp0078541
16.4
142
4.63
Q8IPM8
NP_730800
AAN13292
cpx-PAA
FBpp0302734
14.8
125
4.50
NP_001097678
ABW08594
cpx-PAB
FBpp0307585
17.6
157
4.53
A0A0B4KG19
NP_001262248
AGB95631
cpx-PC
FBpp0078540
16.2
139
4.63
E1JJ37
NP_730803
AAN13295
cpx-PE
FBpp0078542
16.0
138
4.95
NP_525019
AAN13291
cpx-PG
FBpp0078537
16.4
142
4.63
Q8IPM8
NP_730801
AAN13293
cpx-PH
FBpp0078539
16.4
142
4.63
Q8IPM8
NP_730804
AAN13296
cpx-PI
FBpp0078538
16.4
142
4.63
Q8IPM8
NP_730802
AAN13294
cpx-PJ
FBpp0099936
9.9
83
4.50
A0A1B2AKT6
NP_001014603
AAX52938
cpx-PK
FBpp0099938
9.9
83
4.50
A0A1B2AKT6
NP_001014602
AAX52939
cpx-PL
FBpp0112249
15.0
128
4.50
NP_001097673
ABW08589
cpx-PM
FBpp0112250
16.2
139
4.63
E1JJ37
NP_001097674
ABW08590
cpx-PO
FBpp0112252
16.2
139
4.63
E1JJ37
NP_001097676
ABW08592
cpx-PP
FBpp0112253
16.2
139
4.63
E1JJ37
NP_001097677
ABW08593
cpx-PS
FBpp0290013
16.7
146
4.58
E1JJ32
NP_001163499
ACZ94798
cpx-PU
FBpp0290982
16.5
143
4.58
E1JJ33
NP_001163500
ACZ94799
cpx-PV
FBpp0290983
16.4
142
4.63
Q8IPM8
NP_001163501
ACZ94800
cpx-PW
FBpp0290984
16.2
139
4.63
E1JJ37
NP_001163502
ACZ94801
cpx-PX
FBpp0290985
16.4
142
4.63
Q8IPM8
NP_001163503
ACZ94802
cpx-PY
FBpp0290986
16.5
143
4.58
E1JJ33
NP_001163504
ACZ94803
cpx-PZ
FBpp0302644
11.0
98
4.35
E8NH35
NP_001262247
AGB95630
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

83 aa isoforms: cpx-PJ, cpx-PK
142 aa isoforms: cpx-PA, cpx-PG, cpx-PH, cpx-PI, cpx-PV, cpx-PX
139 aa isoforms: cpx-PC, cpx-PM, cpx-PO, cpx-PP, cpx-PW
143 aa isoforms: cpx-PU, cpx-PY
Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Subunit Structure (UniProtKB)

Binds to the SNARE core complex containing Snap25, synaptobrevin and Syx1A.

(UniProt, Q8IPM8)
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Nucleotide / Polypeptide Records
 
UniProt
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\cpx using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.69

Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
late embryonic stage
larval central nervous system

Comment: Expression specific to cpx isoform 7A.

(Buhl et al., 2013)
larval central nervous system | faint

Comment: Expression specific to cpx transcript cpx-RE (isoform 7B).

(Buhl et al., 2013)
RT-PCR
Stage
Tissue/Position (including subcellular localization)
Reference
third instar larval stage
organism

Comment: Expression specific to cpx isoform 7A.

(Buhl et al., 2013)
organism | faint

Comment: Expression specific to cpx transcript cpx-RE (isoform 7B).

(Buhl et al., 2013)
adult stage
organism | intense

Comment: Expression specific to cpx isoform 7A.

(Buhl et al., 2013)
organism

Comment: Expression specific to cpx transcript cpx-RE (isoform 7B).

(Buhl et al., 2013)
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
third instar larval stage
embryonic/larval neuromuscular junction
(Buhl et al., 2013)
embryonic/larval neuromuscular junction
(Huntwork and Littleton, 2007)
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage
adult head
(Aradska et al., 2015)
western blot
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage
adult head
(Buhl et al., 2013)
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
located_in cytosol
inferred from high throughput direct assay
(Lye et al., 2014)
located_in membrane
inferred from direct assay
(Iyer et al., 2013)
located_in neuromuscular junction
inferred from direct assay
(Lowe et al., 2014)
located_in presynaptic active zone
inferred from direct assay
(Iyer et al., 2013)
located_in synaptic vesicle membrane
inferred from mutant phenotype
(Scholz et al., 2019)
located_in terminal bouton
inferred from direct assay
(Buhl et al., 2013, Cho et al., 2010)
located_in type I terminal bouton
inferred from direct assay
(Mahoney et al., 2016)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\cpx in JBrowse
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 27 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 30 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of cpx
Transgenic constructs containing regulatory region of cpx
Aberrations (Deficiencies and Duplications) ( 0 )
Inferred from experimentation ( 0 )
Inferred from location ( 10 )
Variants
Variant Molecular Consequences
Alleles Representing Disease-Implicated Variants
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
partially lethal
partially lethal - majority die
partially lethal - majority die, with Scer\GAL4pnr-MD237
viable
viable, with Dcr-2UAS.cDa, Scer\GAL4elav.PLu
viable, with Scer\GAL4tin.CΔ4
Sterility
Allele
fertile
sterile
Other Phenotypes
Allele
abnormal locomotor behavior
abnormal locomotor behavior | larval stage (with cpx1257)
abnormal locomotor behavior | larval stage (with cpxSH1)
abnormal neuroanatomy | larval stage (with cpx1257)
abnormal neuroanatomy | larval stage (with cpxSH1)
abnormal neurophysiology
abnormal neurophysiology, with Scer\GAL4elav-C155, cpxK75A,Y76A.UAS.Tag:MYC
abnormal neurophysiology, with Scer\GAL4elav-C155, cpxSH1
abnormal neurophysiology, with Scer\GAL4elav-C155, cpxΔCAAX.UAS.Tag:MYC
abnormal neurophysiology | adult stage, with Scer\GAL4exex-Gal4
abnormal neurophysiology | adult stage (with Df(3R)Exel6140)
abnormal neurophysiology | larval stage (with cpx1257)
abnormal neurophysiology | larval stage (with cpxSH1)
abnormal neurophysiology | larval stage (with Df(3R)Exel6140)
abnormal neurophysiology | third instar larval stage
abnormal neurophysiology | third instar larval stage, with cpxC140W.UAS.7A
abnormal neurophysiology | third instar larval stage, with cpxSH1
abnormal neurophysiology | third instar larval stage, with Scer\GAL4C164
abnormal neurophysiology | third instar larval stage, with Scer\GAL4elav-C155
abnormal neurophysiology | third instar larval stage, with Scer\GAL4elav-C155, cpxS126A.UAS.7B
abnormal neurophysiology | third instar larval stage, with Scer\GAL4elav-C155, cpxS126D.UAS.7B
abnormal neurophysiology | third instar larval stage, with Scer\GAL4elav-C155, cpxSH1
abnormal neurophysiology | third instar larval stage, with Scer\GAL4nSyb.PS
flightless, with Scer\GAL4Mef2.PR
some die during pupal stage, with Scer\GAL4pnr-MD237
uncoordinated | adult stage
uncoordinated | heat sensitive (with Df(3R)Exel6140)
visible, with Scer\GAL4pnr-MD237
wild-type
Phenotype manifest in
Allele
adult motor neuron, with Scer\GAL4exex-Gal4
adult neuromuscular junction (with Df(3R)Exel6140)
embryonic/larval motor neuron (with cpx1257)
embryonic/larval motor neuron (with cpxSH1)
embryonic/larval motor neuron | third instar larval stage
embryonic/larval neuromuscular junction (with cpx1257)
embryonic/larval neuromuscular junction (with cpxSH1)
embryonic/larval neuromuscular junction | larval stage (with Df(3R)Exel6140)
embryonic/larval neuromuscular junction | third instar larval stage
embryonic/larval neuromuscular junction | third instar larval stage, with Scer\GAL4elav-C155
embryonic/larval neuromuscular junction | third instar larval stage, with Scer\GAL4elav-C155, cpxK75A,Y76A.UAS.Tag:MYC
embryonic/larval neuromuscular junction | third instar larval stage, with Scer\GAL4elav-C155, cpxSH1
embryonic/larval neuromuscular junction | third instar larval stage, with Scer\GAL4elav-C155, cpxΔCAAX.UAS.Tag:MYC
embryonic/larval neuromuscular junction | third instar larval stage, with Scer\GAL4nSyb.PS
mesonotum, with Scer\GAL4pnr-MD237
muscle cell of A1-7 ventral longitudinal muscle 3 (with cpx1257)
muscle cell of A1-7 ventral longitudinal muscle 3 (with cpxSH1)
neuromuscular junction | third instar larval stage
neuromuscular junction | third instar larval stage, with cpxC140W.UAS.7A
neuromuscular junction | third instar larval stage, with cpxSH1
neuromuscular junction | third instar larval stage, with Scer\GAL4C164
neuromuscular junction | third instar larval stage | temperature conditional, with Scer\GAL4elav-C155, cpxS126A.UAS.7B
neuromuscular junction | third instar larval stage | temperature conditional, with Scer\GAL4elav-C155, cpxS126D.UAS.7B
neuromuscular junction | third instar larval stage | temperature conditional, with Scer\GAL4elav-C155, cpxSH1
NMJ bouton | adult stage, with Scer\GAL4exex-Gal4
NMJ bouton | increased number
NMJ bouton | third instar larval stage
NMJ bouton | third instar larval stage, with Scer\GAL4C164
NMJ bouton | third instar larval stage | temperature conditional, with Scer\GAL4elav-C155, cpxS126A.UAS.7B
NMJ bouton | third instar larval stage | temperature conditional, with Scer\GAL4elav-C155, cpxS126D.UAS.7B
NMJ bouton | third instar larval stage | temperature conditional, with Scer\GAL4elav-C155, cpxSH1
presynaptic active zone
presynaptic active zone | adult stage, with Scer\GAL4exex-Gal4
presynaptic active zone | larval stage (with cpx1257)
presynaptic active zone | larval stage (with cpxSH1)
synaptic vesicle | larval stage (with cpx1257)
synaptic vesicle | larval stage (with cpxSH1)
T-bar | larval stage (with cpx1257)
T-bar | larval stage (with cpxSH1)
ventral longitudinal muscle cell | third instar larval stage
ventral longitudinal muscle cell | third instar larval stage, with cpxC140W.UAS.7A
ventral longitudinal muscle cell | third instar larval stage, with cpxSH1
Orthologs
Downloads
Download All DIOPT Orthologs
Human Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Homo sapiens (Human) (4)
Hsap\CPLX1
10 of 14
Yes
Yes
Hsap\CPLX2
8 of 14
No
Yes
Hsap\CPLX3
2 of 14
No
Yes
Hsap\CPLX4
2 of 14
No
Yes
Model Organism Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Rattus norvegicus (Norway rat) (4)
Rnor\Cplx1
10 of 14
Yes
Yes
 
1  
Rnor\Cplx2
8 of 14
No
Yes
Rnor\Cplx3
2 of 14
No
Yes
Rnor\Cplx4
2 of 14
No
Yes
Mus musculus (laboratory mouse) (4)
Mmus\Cplx1
10 of 14
Yes
Yes
 
9  
Mmus\Cplx2
8 of 14
No
Yes
 
1  
Mmus\Cplx3
2 of 14
No
Yes
 
2  
Mmus\Cplx4
2 of 14
No
Yes
 
2  
Xenopus tropicalis (Western clawed frog) (7)
Xtro\cplx1
7 of 13
Yes
Yes
Xtro\cplx2
7 of 13
Yes
Yes
Xtro\cplx4
1 of 13
No
Yes
Xtro\ing5
1 of 13
No
No
Xtro\p2ry2
1 of 13
No
No
Xtro\vps18
1 of 13
No
No
Xtro\XB5845204
1 of 13
No
Yes
Danio rerio (Zebrafish) (11)
Drer\cplx2l
9 of 14
Yes
Yes
Drer\cplx2
8 of 14
No
Yes
Drer\cplx3a
2 of 14
No
Yes
Drer\cplx3b
2 of 14
No
Yes
Drer\cplx4a
2 of 14
No
Yes
Drer\cplx4b
2 of 14
No
Yes
Drer\cplx4c
2 of 14
No
Yes
Drer\LOC100149700
2 of 14
No
Yes
Drer\LOC563082
1 of 14
No
Yes
Drer\LOC108180184
1 of 14
No
Yes
Drer\si:ch211-81a5.8
1 of 14
No
Yes
Caenorhabditis elegans (Nematode, roundworm) (2)
Cele\cpx-1
11 of 14
Yes
Yes
Cele\cpx-2
2 of 14
No
Yes
Anopheles gambiae (African malaria mosquito) (1)
Agam\AgaP_AGAP003509
9 of 12
Yes
Yes
Arabidopsis thaliana (thale-cress) (0)
Saccharomyces cerevisiae (Brewer's yeast) (0)
Schizosaccharomyces pombe (Fission yeast) (0)
Escherichia coli (enterobacterium) (0)
Other Organism Orthologs (via OrthoDB)
Data provided directly from OrthoDB:cpx. Refer to their site for version information.
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v9.1)
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 1 )
    Human Ortholog
    Disease
    Evidence
    References
    CPLX1; complexin 1
    model of  developmental and epileptic encephalopathy 63
    IEA
    (FlyBase, 2019-)
    Modifiers Based on Experimental Evidence ( 1 )
    Allele
    Disease
    Interaction
    References
    cpxUAS.cHa
    exacerbates  tauopathy
    modeled by Hsap\MAPTUAS.cWa
    (Feuillette et al., 2020)
    Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    CPLX1; complexin 1
    10 of 14
    605032
    CPLX2; complexin 2
    8 of 14
    605033
        Functional Complementation Data
        Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
        Dmel gene
        Ortholog showing functional complementation
        Supporting References
        cpx
        Mmus\Cplx1
        (Buhl et al., 2013, Cho et al., 2010)
        cpx
        Mmus\Cplx2
        (Cho et al., 2010)
        cpx
        Mmus\Cplx3
        (Cho et al., 2010, Xue et al., 2009)
        cpx
        Mmus\Cplx4
        (Buhl et al., 2013, Cho et al., 2010)
        cpx
        Rnor\Cplx1
        (Xue et al., 2009)
        Interactions
        Summary of Physical Interactions
        Summary of Genetic Interactions
        Interaction Browsers
        View in FlyBase Interactions Browser   View in MIST tool (external link)
        Please look at the allele data for full details of the genetic interactions
        Starting gene(s)
        Interaction type
        Interacting gene(s)
        Reference
        cpx
        suppressible
        brp
        (Scholz et al., 2019)
        cpx
        enhanceable
        para
        (Banerjee et al., 2021)
        cpx
        suppressible
        Syt4
        (Cho et al., 2015)
        cpx
        suppressible
        wit
        (Cho et al., 2015)
        Starting gene(s)
        Interaction type
        Interacting gene(s)
        Reference
        brp
        enhanceable
        cpx
        (Scholz et al., 2019)
        brp
        suppressible
        cpx
        (Scholz et al., 2019)
        External Data
        Subunit Structure (UniProtKB)
        Binds to the SNARE core complex containing Snap25, synaptobrevin and Syx1A.
        (UniProt, Q8IPM8 )
        Linkouts
        BioGRID - A database of protein and genetic interactions.
        DroID - A comprehensive database of gene and protein interactions.
        MIST (protein-protein) - An integrated Molecular Interaction Database
        Pathways
        Signaling Pathways (FlyBase)
        Metabolic Pathways
        FlyBase
        External Links
        External Data
        Class of Gene
        Genomic Location and Detailed Mapping Data
        Chromosome (arm)
        3R
        Recombination map
        3-47.1
        Cytogenetic map
        82A1-82A3
        Sequence location
        FlyBase Computed Cytological Location
        Cytogenetic map
        Evidence for location
        82A1-82A3
        Limits computationally determined from genome sequence between P{PZ}l(3)0273302733 and P{EP}EP974
        Experimentally Determined Cytological Location
        Cytogenetic map
        Notes
        References
        Experimentally Determined Recombination Data
        Location

        3-47.1

        (FlyBase, 2016)

        3-44.1

        (Comeron et al., 2012)
        Left of (cM)
        Right of (cM)
        Notes
        Stocks and Reagents
        Stocks (54)
        Genomic Clones (26)
        cDNA Clones (394)
         

        Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

        cDNA clones, fully sequenced
        BDGP DGC clones
        Other clones
        Drosophila Genomics Resource Center cDNA clones

        For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

        cDNA Clones, End Sequenced (ESTs)
        BDGP DGC clones
        Other clones
        RNAi and Array Information
        Linkouts
        DRSC - Results frm RNAi screens
        Antibody Information
        Laboratory Generated Antibodies
         

        polyclonal

        (Huntwork and Littleton, 2007)
        Commercially Available Antibodies
         
        Cell Line Information
        Publicly Available Cell Lines
         
          Other Stable Cell Lines
           
            Other Comments

            Duplicate transcripts identified and eliminated during the migration of annotations from the release 5 genome assembly to the release 6 assembly.

            (FlyBase Genome Annotators, 2014)

            New annotation (CG32490) in release 3 of the genome annotation.

            (FlyBase Genome Annotators, 2002-2003)
            Relationship to Other Genes
            Source for database merge of

            Source for merge of: complexin BcDNA:RH26459

            (FlyBase, 1996-)

            Source for merge of: complexin BcDNA:RH31085

            (FlyBase, 1996-)

            Source for merge of: complexin BcDNA:GH27718

            (FlyBase, 1996-)
            Additional comments

            mir-929 is located within cpx.

            (Stark et al., 2007)

            Source for merge of complexin BcDNA:RH26459 was a shared cDNA ( date:030728 ).

            (FlyBase, 1996-)

            Source for merge of complexin BcDNA:RH31085 was a shared cDNA ( date:030728 ).

            (FlyBase, 1996-)

            Source for merge of complexin BcDNA:GH27718 was a shared cDNA ( date:030728 ).

            (FlyBase, 1996-)
            Nomenclature History
            Source for database identify of
            Nomenclature comments
            Etymology
            Synonyms and Secondary IDs (12)
            Reported As
            Symbol Synonym
            BcDNA:GH27718
            BcDNA:HL04936
            (Fujii and Amrein, 2002)
            BcDNA:RH26459
            BcDNA:RH31085
            CG32490
            (Neuert et al., 2017, Aradska et al., 2015, Rieder et al., 2015, Ni et al., 2010.12.1, Metaxakis et al., 2005)
            CPX
            (Luo et al., 2018, Uytterhoeven et al., 2015)
            Cpx
            (Bulgari et al., 2023, Bykhovskaia, 2022, Newman et al., 2022, Quiñones-Frías and Littleton, 2021, Sauvola and Littleton, 2021, Hope et al., 2020, Lowe et al., 2014, Bykhovskaia et al., 2013, Guan et al., 2011, Barber et al., 2009, Rodal et al., 2008)
            DmCpx
            (Buhl et al., 2013, Cho et al., 2010)
            cpx
            (Li et al., 2025, Aziz et al., 2024, Corthals et al., 2023, Titlow et al., 2023, Voutyraki et al., 2023, Astacio et al., 2022, Courtney et al., 2022, Baker et al., 2021, Brady et al., 2021, Hall et al., 2021, Pang et al., 2021, Uçkun et al., 2021, Feuillette et al., 2020, Takai et al., 2020, Scholz et al., 2019, Kuznetsova et al., 2018, Luo et al., 2018, Vernon et al., 2018, Wang et al., 2018, Neuert et al., 2017, Sabeva and Bykhovskaia, 2017, Sabeva et al., 2017, Gene Disruption Project members, 2016-, Mahoney et al., 2016, McNew, 2016.3.5, Cho et al., 2015, Harris and Littleton, 2015, Matthews et al., 2015, McNew, 2015.12.8, McNew, 2015.12.21, Rieder et al., 2015, Ashwal-Fluss et al., 2014, Chatterjee et al., 2014, Chen et al., 2014, Cho et al., 2014, Choi et al., 2014, FlyBase Genome Annotators, 2014, Buhl et al., 2013, Iyer et al., 2013, Jorquera et al., 2012, Nfonsam et al., 2012, Rodriguez et al., 2012, Savva et al., 2012, Cho et al., 2010, Huntwork and Littleton, 2007, Stark et al., 2007, Larsen et al., 2006)
            dmCplx
            (Xue et al., 2009)
            Name Synonyms
            Complexin
            (Cho et al., 2015, Uytterhoeven et al., 2015, Cho et al., 2014, Lowe et al., 2014, Jorquera et al., 2012, Guan et al., 2011, Xue et al., 2009)
            complexin
            (Mahoney et al., 2016, Buhl et al., 2013, Bykhovskaia et al., 2013, Cho et al., 2010, Dalton et al., 2010, Huntwork and Littleton, 2007, Burnette et al., 2005, Giot et al., 2003, Pabst, 2000.4.25)
            Secondary FlyBase IDs
            • FBan0032490
            • FBgn0052490
            • FBgn0063217
            • FBgn0063605
            • FBgn0064822
            • FBgn0064830
            Datasets (0)
            Study focus (0)
            Experimental Role
            Project
            Project Type
            Title
            Study result (0)
            Result
            Result Type
            Title
            External Crossreferences and Linkouts ( 132 )
            Sequence Crossreferences
            NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
            GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
            GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
            RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
            UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.
            UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
            UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
            Other crossreferences
            AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.
            DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
            EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
            FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
            FlyMine - An integrated database for Drosophila genomics
            InterPro - A database of protein families, domains and functional sites
            KEGG Genes - Molecular building blocks of life in the genomic space.
            MARRVEL_MODEL - MARRVEL (model organism gene)
            Linkouts
            BioGRID - A database of protein and genetic interactions.
            Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
            DroID - A comprehensive database of gene and protein interactions.
            DRSC - Results frm RNAi screens
            Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
            FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
            FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
            Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
            Flygut - An atlas of the Drosophila adult midgut
            FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.
            iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
            Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
            MIST (protein-protein) - An integrated Molecular Interaction Database
            Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
            References (120)