FlyBase Gene Report: Dmel\Stim

General Information

Symbol

Dmel\Stim

Species

D. melanogaster

Name

Stromal interaction molecule

Annotation Symbol

CG9126

Feature Type

protein_coding_gene

FlyBase ID

FBgn0045073

Gene Model Status

Current

Stock Availability

15 publicly available

Gene Summary

Stromal interaction molecule (Stim) encodes an endomasplic reticulum-membrane protein that is an essential component of the store-operated calcium entry mechanism, which in neurons regulates flight. [Date last reviewed: 2018-11-08] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
UniProtKB
Interactive Fly

Also Known As

dSTIM, STIM1, D-Stim

Key Links

Genomic Location

Cytogenetic map

14A1-14A1

Sequence location

X:15,934,495..15,940,451 [-]

Recombination map

1-52

RefSeq locus

NC_004354 REGION:15934495..15940451

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (15 terms)

Molecular Function (4 terms)

Terms Based on Experimental Evidence (3 terms)

CV Term

Evidence

References

enables calcium ion binding

inferred from direct assay

(Roos et al., 2005)

enables calcium-induced calcium release activity

inferred from mutant phenotype

(Roos et al., 2005)

enables store-operated calcium channel activity

inferred from mutant phenotype

(Roos et al., 2005)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

enables calcium channel regulator activity

inferred from biological aspect of ancestor with PANTHER:PTN001031746

(GO Reference Genome Project, 2011-)

inferred from electronic annotation with InterPro:IPR037608

(InterPro Project Members, 2004-)

enables calcium ion binding

inferred from biological aspect of ancestor with PANTHER:PTN001031746

(GO Reference Genome Project, 2011-)

Biological Process (6 terms)

Terms Based on Experimental Evidence (5 terms)

CV Term

Evidence

References

involved_in chaeta development

inferred from mutant phenotype

(Eid et al., 2008)

involved_in detection of calcium ion

inferred from direct assay

(Roos et al., 2005)

involved_in imaginal disc-derived wing morphogenesis

inferred from mutant phenotype

(Eid et al., 2008)

involved_in regulation of calcium ion transport

inferred from direct assay

(Roos et al., 2005)

involved_in store-operated calcium entry

inferred from mutant phenotype

(Gwack et al., 2007)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

involved_in intracellular calcium ion homeostasis

inferred from biological aspect of ancestor with PANTHER:PTN001031746

(GO Reference Genome Project, 2011-)

involved_in store-operated calcium entry

inferred from biological aspect of ancestor with PANTHER:PTN001031746

(GO Reference Genome Project, 2011-)

Cellular Component (5 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

located_in endomembrane system

inferred from high throughput direct assay

(Tan et al., 2009)

located_in endoplasmic reticulum-plasma membrane contact site

inferred from direct assay

(Pérez-Moreno et al., 2023)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

is_active_in endoplasmic reticulum

inferred from biological aspect of ancestor with PANTHER:PTN001031746

(GO Reference Genome Project, 2011-)

located_in membrane

non-traceable author statement

(Roos et al., 2005)

is_active_in plasma membrane

inferred from biological aspect of ancestor with PANTHER:PTN001031746

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

CALCIUM RELEASE-ACTIVATED CALCIUM CHANNEL

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

Gene Group (FlyBase)

CALCIUM RELEASE-ACTIVATED CALCIUM CHANNEL -: Ca[2+] release-activated Ca[2+] (CRAC) channels function in store-operated Ca[2+] entry, a mechanism for Ca[2+] entry across the plasma membrane (PM) into the endoplasmic reticulum (ER)) Ca[2+] stores. CRAC channels are highly Ca[2+] selective and are composed of Orai pore-forming subunits and the stromal interaction molecule (Stim), a Ca[2+] sensor. When ER Ca[2+] stores are depleted, a conformational change in ER-membrane localized Stim brings it into contact with PM-localized Orai to form a complex, allowing the movement of Ca[2+] from the extracellular milieu directly into the ER lumen. (Adapted from FBrf0251360, FBrf0214207 and PMID:27130253.)

Protein Function (UniProtKB)

Plays a role in mediating Ca(2+) influx following depletion of intracellular Ca(2+) stores.

(UniProt, P83094)

Summary (Interactive Fly)

ER-Ca2+ sensor required for store-operated Ca2+ entry - dimerizes and undergoes structural rearrangements facilitating binding Orai - regulates the release of neuropeptides - regulates lipid mobilization in response to Adipokinetic hormone and insulin signaling in fat body - regulates synaptic release from pupal dopaminergic neurons allowing for sustained flight

Interactive Fly

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Stim for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry P83094)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.50

Annotated transcripts do not represent all supported alternative splices within 5' UTR.

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.39

Gene model reviewed during 5.55

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Stim-RA

FBtr0074159

NM_078633

2255

570

Stim-RD

FBtr0303567

NM_206747

2141

561

Stim-RF

FBtr0308705

NM_001258774

2319

117

Stim-RG

FBtr0345134

NM_001298380

961

117

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

Stim-PA

FBpp0073955

64.8

570

6.41

FBpp0292600

63.7

561

6.48

FBpp0300911

13.0

117

7.86

FBpp0311355

13.0

117

7.86

M9NF82

NP_001285309

AHN59779

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

117 aa isoforms: Stim-PF, Stim-PG

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Stim using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.87

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 1 -- 5

organism | ubiquitous

Comment: except pole cells

(Eid et al., 2008)

embryonic stage 4 -- 6

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 7

ventral furrow

(Eid et al., 2008)

cephalic furrow

(Eid et al., 2008)

embryonic stage 7 -- 8

organism | faint | ubiquitous

(Fisher et al., 2012)

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 8 -- 11

anterior midgut primordium

(Eid et al., 2008)

posterior midgut primordium

(Eid et al., 2008)

embryonic stage 8 -- 13

ectoderm

(Eid et al., 2008)

embryonic stage 9 -- 10

organism | faint | ubiquitous

(Fisher et al., 2012)

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 11 -- 12

organism | faint | ubiquitous

(Fisher et al., 2012)

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 13

embryonic/larval midgut

(Eid et al., 2008)

embryonic Malpighian tubule

(Eid et al., 2008)

embryonic/larval salivary gland

(Eid et al., 2008)

ventral midline of embryo | subset

(Eid et al., 2008)

larval central nervous system | restricted

(Eid et al., 2008)

embryonic stage 13 -- 16

organism | faint | ubiquitous

(Fisher et al., 2012)

organism | ubiquitous

(Fisher et al., 2012)

third instar larval stage

wing disc | uniform

(Eid et al., 2008)

eye disc | uniform

(Eid et al., 2008)

leg disc | uniform

(Eid et al., 2008)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 5

organism

(Eid et al., 2008)

embryonic stage 15

ventral midline of embryo | subset

(Eid et al., 2008)

midline glial cell

(Eid et al., 2008)

embryonic/larval salivary gland

(Eid et al., 2008)

third instar larval stage

wing disc | uniform

(Eid et al., 2008)

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in endomembrane system

inferred from high throughput direct assay

(Tan et al., 2009)

located_in endoplasmic reticulum-plasma membrane contact site

inferred from direct assay

(Pérez-Moreno et al., 2023)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Stim in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Stim

View exonic expression by tissue for Dmel\Stim

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0045073

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

32556/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0045073

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0045073

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0045073

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG9126

Flygut - An atlas of the Drosophila adult midgut

FBgn0045073

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\Stim

Stages(s) 1-3
Stages(s) 4-6
Stages(s) 7-8
Stages(s) 13-16

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 3 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 16 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Stim

Transgenic constructs containing regulatory region of Stim

Aberrations (Deficiencies and Duplications) ( 1 )

Inferred from experimentation ( 1 )

Gene disrupted in

Df(1)sd72a

(Eid et al., 2008)

Inferred from location ( 10 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal, with Dcr-2^UAS.cDa, Scer\GAL4^e22c, Scer\GAL4^sqh.PW

lethal, with Scer\GAL4^da.G32

Stim^UAS.cEa

lethal, with Scer\GAL4^e22c

Stim^UAS.cEa

lethal, with Scer\GAL4^αTub84B.PL

lethal | male, with Scer\GAL4^elav-C155, Spyo\Cas9^{UAS.Tag:NLS(Ude)}

Stim^{sgRNA.dual.snRNA:U6:96Ab}

lethal - all die before end of larval stage, with Scer\GAL4^da.G32

Stim^RNAi.cEa.UAS

lethal - all die before end of larval stage, with Scer\GAL4^e22c

Stim^RNAi.cEa.UAS

lethal - all die before end of larval stage | recessive

Stim^ko

partially lethal, with Scer\GAL4^ple.TH-A, Spyo\Cas9^{UAS.Tag:NLS(Ude)}

Stim^{sgRNA.dual.snRNA:U6:96Ab}

partially lethal - majority die, with Scer\GAL4^elav-C155, Spyo\Cas9^{UAS.Tag:NLS(Ude)}

Stim^{sgRNA.dual.snRNA:U6:96Ab}

partially lethal - majority die, with Scer\GAL4^nSyb.PS, Spyo\Cas9^{UAS.Tag:NLS(Ude)}

Stim^{sgRNA.dual.snRNA:U6:96Ab}

partially lethal - majority die, with Scer\GAL4^ple.PF, Spyo\Cas9^{UAS.Tag:NLS(Ude)}

Stim^{sgRNA.dual.snRNA:U6:96Ab}

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Stim^GD16187

viable, with Scer\GAL4^Mef2.PR

viable, with Scer\GAL4^NP5440

Stim^HMC03651

viable, with Scer\GAL4^pnr-MD237

Stim^GD16187

Other Phenotypes

Allele

abnormal adult locomotory behavior | conditional, with Scer\GAL4^Mef2.PR

abnormal cell adhesion | oogenesis, with Scer\GAL4^slbo.2.6

Stim^HMC03651

abnormal developmental rate

Stim^ko

abnormal developmental rate, with Scer\GAL4^elav-C155, Spyo\Cas9^{UAS.Tag:NLS(Ude)}

Stim^{sgRNA.dual.snRNA:U6:96Ab}

abnormal developmental rate, with Scer\GAL4^nSyb.PS, Spyo\Cas9^{UAS.Tag:NLS(Ude)}

Stim^{sgRNA.dual.snRNA:U6:96Ab}

abnormal developmental rate, with Scer\GAL4^ple.PF, Spyo\Cas9^{UAS.Tag:NLS(Ude)}

Stim^{sgRNA.dual.snRNA:U6:96Ab}

abnormal feeding behavior | adult stage, with Scer\GAL4^fat

Stim^RNAi.cEa.UAS

abnormal feeding behavior | adult stage | RU486 conditional, with Scer\GAL4^da.Switch.PT

Stim^RNAi.cEa.UAS

abnormal flight, with Scer\GAL4^ple.PF

Stim^GD16187

abnormal flight, with Scer\GAL4^ple.TH-D

Stim^GD16187

abnormal flight | adult stage, with Scer\GAL4^elav-C155

Stim^GD16187

abnormal locomotor behavior | adult stage, with Scer\GAL4^fat

Stim^RNAi.cEa.UAS

abnormal neuroanatomy, with Scer\GAL4²²¹

Stim^JF02567

abnormal neuroanatomy, with Scer\GAL4^ppk.PG

Stim^JF02567

abnormal neurophysiology, with Scer\GAL4^ple.PF

Stim^GD16187

abnormal size | adult stage | progressive, with Scer\GAL4^fat

Stim^RNAi.cEa.UAS

abnormal starvation stress response | adult stage, with Scer\GAL4^fat

Stim^RNAi.cEa.UAS

decreased body size | larval stage

Stim^ko

decreased rate of movement | larval stage, with Scer\GAL4^da.G32

Stim^RNAi.cEa.UAS

decreased rate of movement | larval stage, with Scer\GAL4^e22c

Stim^RNAi.cEa.UAS

increased cell death, with Scer\GAL4^Bx-MS1096

Stim^UAS.cEa

increased cell size | adult stage | progressive, with Scer\GAL4^fat

Stim^RNAi.cEa.UAS

long lived, with Scer\GAL4^fat

Stim^RNAi.cEa.UAS

long lived | calorie restriction conditional, with Scer\GAL4^fat

Stim^RNAi.cEa.UAS

some die during larval stage, with Scer\GAL4^da.G32

Stim^RNAi.cEa.UAS

some die during larval stage, with Scer\GAL4^e22c

Stim^RNAi.cEa.UAS

visible, with Df(1)sd72a, Scer\GAL4^en-e16E

Stim^RNAi.cEa.UAS

visible, with Scer\GAL4^32B

Stim^UAS.cEa

visible, with Scer\GAL4^Bx-MS1096

visible, with Scer\GAL4^en-e16E

visible, with Scer\GAL4^ey.PH

Stim^UAS.cEa

visible, with Scer\GAL4^GMR.PF

Stim^UAS.cEa

visible, with Scer\GAL4^lz-gal4

Stim^UAS.cEa

visible, with Scer\GAL4^pnr-MD237

visible, with Scer\GAL4^sca.PU

Stim^UAS.cEa

visible, with Scer\GAL4^sd-SG29.1

Phenotype manifest in

Allele

abdominal dorsal multidendritic neuron ddaC | third instar larval stage, with Scer\GAL4^ppk.PG

Stim^JF02567

abdominal dorsal multidendritic neuron ddaD | third instar larval stage, with Scer\GAL4²²¹

Stim^JF02567

abdominal dorsal multidendritic neuron ddaE | third instar larval stage, with Scer\GAL4²²¹

Stim^JF02567

adult abdomen | progressive, with Scer\GAL4^fat

Stim^RNAi.cEa.UAS

adult abdominal fat mass | progressive, with Scer\GAL4^fat

Stim^RNAi.cEa.UAS

anterior wing margin, with Scer\GAL4^32B

Stim^UAS.cEa

anterior wing margin, with Scer\GAL4^sd-SG29.1

Stim^UAS.cEa

border follicle cell, with Scer\GAL4^slbo.2.6

Stim^HMC03651

campaniform sensillum | ectopic, with Scer\GAL4^Bx-MS1096

Stim^UAS.cEa

dendritic tree | third instar larval stage, with Scer\GAL4²²¹

Stim^JF02567

dendritic tree | third instar larval stage, with Scer\GAL4^ppk.PG

Stim^JF02567

embryonic/larval fat body, with Scer\GAL4^da.G32

Stim^RNAi.cEa.UAS

embryonic/larval fat body, with Scer\GAL4^e22c

Stim^RNAi.cEa.UAS

embryonic/larval fat body | larval stage, with Scer\GAL4^NP5440

Stim^HMC03651

eye, with Scer\GAL4^ey.PH

Stim^UAS.cEa

eye, with Scer\GAL4^GMR.PF

Stim^UAS.cEa

eye, with Scer\GAL4^lz-gal4

Stim^UAS.cEa

fat cell | adult stage | progressive, with Scer\GAL4^fat

Stim^RNAi.cEa.UAS

interommatidial bristle, with Scer\GAL4^GMR.PF

Stim^UAS.cEa

interommatidial bristle, with Scer\GAL4^lz-gal4

Stim^UAS.cEa

interommatidial bristle, with Scer\GAL4^sca.PU

Stim^UAS.cEa

lipid droplet | adult stage | progressive, with Scer\GAL4^fat

Stim^RNAi.cEa.UAS

mesonotum, with Scer\GAL4^pnr-MD237

Stim^RNAi.cEa.UAS

microchaeta, with Scer\GAL4^Bx-MS1096

Stim^UAS.cEa

microchaeta, with Scer\GAL4^pnr-MD237

Stim^UAS.cEa

microchaeta, with Scer\GAL4^sca.PU

Stim^UAS.cEa

microchaeta & mesothoracic tergum, with Scer\GAL4^pnr-MD237

Stim^RNAi.cEa.UAS

ommatidium, with Scer\GAL4^ey.PH

Stim^UAS.cEa

posterior wing margin, with Scer\GAL4^32B

Stim^UAS.cEa

posterior wing margin, with Scer\GAL4^en-e16E

Stim^UAS.cEa

posterior wing margin, with Scer\GAL4^sd-SG29.1

Stim^UAS.cEa

trichogen cell | increased number, with Scer\GAL4^pnr-MD237

Stim^GD16187

wing, with Scer\GAL4^Bx-MS1096

Stim^UAS.cEa

wing disc, with Scer\GAL4^Bx-MS1096

Stim^UAS.cEa

wing hair, with Scer\GAL4^Bx-MS1096

Stim^UAS.cEa

wing margin bristle, with Scer\GAL4^Bx-MS1096

Stim^UAS.cEa

wing margin bristle | ectopic, with Scer\GAL4^sca.PU

Stim^UAS.cEa

wing vein, with Df(1)sd72a, Scer\GAL4^en-e16E

Stim^RNAi.cEa.UAS

wing vein, with Scer\GAL4^Bx-MS1096

Stim^RNAi.cEa.UAS

wing vein, with Scer\GAL4^en-e16E

Stim^RNAi.cEa.UAS

wing vein, with Scer\GAL4^sd-SG29.1

Stim^RNAi.cEa.UAS

wing vein | ectopic, with Scer\GAL4^32B

Stim^UAS.cEa

wing vein | ectopic, with Scer\GAL4^sd-SG29.1

Stim^UAS.cEa

wing vein L5 | distal, with Scer\GAL4^Bx-MS1096

Stim^UAS.cEa

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (2)

Hsap\STIM1

13 of 14

Yes

Hsap\STIM2

11 of 14

Yes

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (2)

Rnor\Stim1

13 of 14

Yes

Rnor\Stim2

11 of 14

Yes

Mus musculus (laboratory mouse) (2)

Mmus\Stim1

13 of 14

Yes

Mmus\Stim2

10 of 14

Yes

Xenopus tropicalis (Western clawed frog) (4)

Xtro\stim1

10 of 13

Yes

Xtro\LOC101730384

6 of 13

Yes

Xtro\stim2

6 of 13

Yes

Xtro\calb1

1 of 13

Danio rerio (Zebrafish) (4)

Drer\stim1a

12 of 14

Yes

Drer\stim1b

11 of 14

Yes

Drer\stim2b

11 of 14

Yes

Drer\stim2a

10 of 14

Yes

Caenorhabditis elegans (Nematode, roundworm) (1)

Cele\stim-1

11 of 14

Yes

Anopheles gambiae (African malaria mosquito) (2)

Agam\AgaP_AGAP000175

12 of 12

Yes

Agam\AgaP_AGAP012978

1 of 12

Yes

Arabidopsis thaliana (thale-cress) (0)

Saccharomyces cerevisiae (Brewer's yeast) (0)

Schizosaccharomyces pombe (Fission yeast) (0)

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Stim. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (0)

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 4 )

Allele

Disease

Evidence

References

Stim^RNAi.cEa.UAS

model of obesity

CEA

(Xu et al., 2019, Baumbach et al., 2014)

Stim^GD16187

model of obesity

CEA

(Xu et al., 2019)

Stim^JF02567

model of dilated cardiomyopathy

CEA

(Petersen et al., 2020)

Stim^{DDAA.UAS.EGFP}

model of hypertrophic cardiomyopathy

CEA

(Petersen et al., 2022)

Potential Models Based on Orthology ( 3 )

Human Ortholog

Disease

Evidence

References

STIM1; stromal interaction molecule 1

model of tubular aggregate myopathy 1

IEA

(FlyBase, 2019-)

model of Stormorken syndrome

IEA

(FlyBase, 2019-)

model of immunodeficiency 10

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 1 )

Allele

Disease

Interaction

References

Stim^UAS.mCherry

ameliorates hereditary spastic paraplegia 12

modeled by Rtnl1¹⁸

(Pérez-Moreno et al., 2023)

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

STIM1; stromal interaction molecule 1

13 of 14

STROMAL INTERACTION MOLECULE 1; STIM1

STIM2; stromal interaction molecule 2

11 of 14

610841

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Stim

protein cross-linking with a bifunctional reagent, western blot

(Penna et al., 2008)

Stim - Orai

anti tag coimmunoprecipitation, anti tag western blot, western blot

(Chakraborty et al., 2016, Yeromin et al., 2006)

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

suppressible

(Xu et al., 2019)

suppressible

(Ma et al., 2020)

suppressible

(Eid et al., 2008)

Stim

suppressible

Rala

(Richhariya et al., 2017)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

suppressible

(Bi et al., 2021)

suppressible

(Agrawal et al., 2010)

Itpr

suppressible

Orai|Stim

(Agrawal et al., 2010)

suppressible

(Eid et al., 2008)

enhanceable

(Ma et al., 2020)

suppressible

(Ma et al., 2020)

suppressible

(Pérez-Moreno et al., 2023)

External Data

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0045073

MIST (protein-protein) - An integrated Molecular Interaction Database

32556

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

1-52

Cytogenetic map

14A1-14A1

Sequence location

X:15,934,495..15,940,451 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

14A1-14A1

Limits computationally determined from genome sequence between P{EP}Gβ13F^EP1071 and P{EP}EP1458&P{EP}EP1522

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

14A1-14A1

(Williams, 2000.12.15)

14A-14A

(determined by in situ hybridisation)

(Williams et al., 2001)

Experimentally Determined Recombination Data

Location

1-52

(FlyBase, 2016)

1-52.4

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (15)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

41756

w^*; P{UAS-Stim.FL}148-15

41757

w^*; P{UAS-Stim.FL}128-10

41758

w^*; P{UAS-Stim.RNAi.E}26-1

602865

w¹¹¹⁸ Stim^ko/FM7a

27263

y¹ v¹; P{TRiP.JF02567}attP2

51685

y¹ v¹; P{TRiP.GLC01785}attP40

41759

w^*; P{UAS-Stim.RNAi.E}8-4/TM3, Sb¹

602866

y¹ v¹; P{U6-Stim.sgRNA.dual}attP40/CyO

606096

w^*; M{UAS-Stim.mCherry}ZH-86Fb/TM6C, Sb¹

VDRC

v106256

P{KK102366}VIE-260B

v47074

w¹¹¹⁸ P{GD16187}v47074

v47073

w¹¹¹⁸; P{GD16187}v47073

More stocks available...

Genomic Clones (9)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (84)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

LD45776

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0045073

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0045073

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Baumbach et al., 2014, Eid et al., 2008)

monoclonal

(Agrawal et al., 2013)

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

New stable cell line derived from S2-unspecified : S2 cell lines expressing circCG32369, circMCPH1, circeIF5B, circrl, circPde11, circCG17715, circzfh2, circTao, circcrol, circdrn, circMeltrin, circpxb, circfru, circdati, circstw, circSarm, circStim, circCG2991, circPvr, circHil, circmub, circsxc, circbnl, and circCG9743 were created. The author reports new stable cell lines S2-circMCPH1, S2-circCG32369, S2-Flag-Ddx56 , and S2-Flag-gw.
(Su et al., 2024)

Other Comments

S2R+ cells treated with dsRNA made from templates generated with primers against this gene show complete inhibition of thapsigargin-induced Ca²⁺ influx, but there is no effect on the filling state of Ca²⁺ stores.

(Feske et al., 2006)

dsRNA made from templates generated with primers against this gene used in a genome-wide dsRNA interference (RNAi) screen to identify regulators of the signalling pathway leading from Ca²⁺-calcineurin to NFAT.

(Gwack et al., 2006)

Treatment of S2 cells with dsRNA made from templates generated with primers directed against this gene inhibits both the early and sustained components of Ca²⁺ entry evoked by thapsigargin at the single-cell level.

(Zhang et al., 2006)

Relationship to Other Genes

Source for database merge of

Additional comments

Annotation CG9126 split into Ranbp16, Stim in release 3 of the genome annotation. In addition, release 3 annotation for Ranbp16 contains sequences corresponding to release 2 annotation CG9136.

(FlyBase Genome Annotators, 2002-2003)

Source for split of: CG9126

(Williams et al., 2001)

Nomenclature History

Source for database identify of

Nomenclature comments

Etymology

Synonyms and Secondary IDs (16)

Reported As

Symbol Synonym

C-Stim

(Penna et al., 2008)

CG9126

(Kanamori et al., 2013, Tsubouchi et al., 2012, Metta and Schlötterer, 2010, Ni et al., 2010.12.1, Keleman et al., 2009.8.5, Venkiteswaran and Hasan, 2009, Roos et al., 2005, Eid, 2001.8.5, Millburn, 2001.8.14)

D-STIM

(Eid, 2001.8.5)

D-Stim

(Williams et al., 2001, Williams, 2000.12.15)

STIM

(Chakraborty and Hasan, 2024, Kasturacharya et al., 2023, Bi et al., 2021, Zhang et al., 2021, Hou et al., 2020, Lewis, 2020, Megha and Hasan, 2020, Toprak et al., 2020, Alavizargar et al., 2018, Hou et al., 2018, Chakraborty et al., 2016, Pathak et al., 2015, Bi et al., 2014, Chakraborty and Hasan, 2012, Cai, 2007, Cai, 2007, Gwack et al., 2006, Putney, 2005)

STIM1

(Wang et al., 2024, Vajente et al., 2020, Nelson et al., 2019, Tirado-Lee et al., 2015)

dSTIM

(Liang et al., 2022, Pathak et al., 2017, Wood and Martin, 2017, Deb and Hasan, 2016, Deb et al., 2016, Jayakumar et al., 2016, Pathak et al., 2015, Hattori et al., 2013, Chorna and Hasan, 2012, Sadaf et al., 2012, Agrawal et al., 2010, Venkiteswaran and Hasan, 2009, Cuttell et al., 2008, Eid et al., 2008)

dStim

(Weiss et al., 2022, Rajan et al., 2017, Richhariya et al., 2017, Gwack et al., 2007, Feske et al., 2006)

dstim

(Cuttell et al., 2008)

stim

(Singh and Agrawal, 2022, Chatterjee and Perrimon, 2021, Schotthöfer and Bohrmann, 2020, Kanamori et al., 2013)

stim1

(Vig et al., 2006)

Name Synonyms

Drosophila Stromal interaction molecule

(Williams, 2000.12.15)

Stromal interaction molecule

(Tsubouchi et al., 2012, Williams, 2000.12.15)

stromal interaction molecule

(Agrawal et al., 2010, Venkiteswaran and Hasan, 2009, Vig et al., 2006, Yeromin et al., 2006, Putney, 2005)

stromal interaction molecule 1

(Bi et al., 2014)

Secondary FlyBase IDs

FBgn0030709

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 52 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

32556

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

P83094

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

P83094

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

P83094

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0045073

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

32556/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0045073

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0045073

FlyMine - An integrated database for Drosophila genomics

FBgn0045073

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG9126

MARRVEL_MODEL - MARRVEL (model organism gene)

32556

Linkouts

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0045073

DroID - A comprehensive database of gene and protein interactions.

FBgn0045073

DRSC - Results frm RNAi screens

FBgn0045073

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0045073

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0045073

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG9126

Flygut - An atlas of the Drosophila adult midgut

FBgn0045073

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC008118

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

MIST (protein-protein) - An integrated Molecular Interaction Database

32556

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

References (137)

The following external sites may use different assemblies or annotations than FlyBase.

-0.87

Report Sections