Gene model reviewed during 5.43
Low-frequency RNA-Seq exon junction(s) not annotated.
Annotated transcripts do not represent all supported alternative splices within 5' UTR.
Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.
Gene model reviewed during 5.46
Gene model reviewed during 5.47
Gene model reviewed during 6.32
Interacts (via N-terminal region) with AGO1 (via Piwi domain); the interaction is essential for localization of AGO1 in P-bodies and for miRNA-mediated silencing. Interacts with pAbp/PABPC1; this interaction interferes with the binding of pAbp to eIF4G and is required for miRNA-mediated silencing. Interacts with CCR4-NOT complex members Not1, Rga/NOT2, twin/CCR4, Pop2 and NOT3/5 and with PAN complex members CG8232/PAN2 and CG11486/PAN3.
The UBA domain is not required for correct subcellular location, gene silencing or interaction with pAbp.
The RRM domain lacks RNA-binding properties and does not bind RNA in vitro. It is not required for P-body localization or for interaction with AGO1 or miRNAs but is required for silencing. May play a role in protein-protein interactions.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\gw using the Feature Mapper tool.
GBrowse - Visual display of RNA-Seq signalsView Dmel\gw in GBrowse 2
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Source for identity of: gw CG31992
FlyBase curator comment: Correspondence with author Jacquier revealed that 'Gawki' = 'gw' (CG31992).
Annotations CG11484, CG9905 merged as CG31992 in release 3 of the genome annotation.
Source for merge of CG11484 CG9905 was a shared cDNA ( date:010720 ).
gw silences expression of bound transcripts by two mechanisms: one involving inhibition of protein expression, and one involving a reduction of mRNA levels.
dsRNA made from templates generated with primers directed against this gene are used to infer a role for this gene in the miRNA pathway.
Loss of gw leads to breakdown of the cortical cytoskeleton and nuclear expansion.
Mutant embryos have severe abnormalities, including disruptions in the precisely timed early embryonic nuclear divisions that normally occur prior to cellularization of the syncytial embryo.
dsRNA has been made from templates generated with primers directed against this gene.
RNAi generated by PCR using primers directed to this gene causes a cell growth and viability phenotype when assayed in Kc167 and S2R+ cells.