FlyBase Gene Report: Dmel\Lasp

General Information

Symbol

Dmel\Lasp

Species

D. melanogaster

Name

Lasp

Annotation Symbol

CG3849

Feature Type

protein_coding_gene

FlyBase ID

FBgn0063485

Gene Model Status

Current

Stock Availability

15 publicly available

Gene Summary

Lasp (Lasp) encodes a member of the nebulin family. It plays subtle roles in modulating the actin cytoskeleton in ovaries, testes, and muscles, by serving as a scaffold protein binding multiple partners, especially actin and myosin. In indirect flight muscles, it contributes to I-band and A-band architecture. [Date last reviewed: 2019-03-14] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Interactive Fly

Key Links

Genomic Location

Cytogenetic map

73B6-73C1

Sequence location

3L:16,676,406..16,711,677 [-]

Recombination map

3-44

RefSeq locus

NT_037436 REGION:16676406..16711677

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (15 terms)

Molecular Function (3 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

enables actin binding

inferred from direct assay

(Suyama et al., 2009)

enables protein binding

inferred from physical interaction with FLYBASE:Actn; FB:FBgn0000667

(Fernandes and Schöck, 2014)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

enables actin filament binding

inferred from biological aspect of ancestor with PANTHER:PTN000867550

(GO Reference Genome Project, 2011-)

Biological Process (7 terms)

Terms Based on Experimental Evidence (7 terms)

CV Term

Evidence

References

involved_in muscle thin filament assembly

inferred from mutant phenotype

(Fernandes and Schöck, 2014)

involved_in pole cell formation

inferred from mutant phenotype

(Suyama et al., 2009)

involved_in positive regulation of pole plasm oskar mRNA localization

inferred from mutant phenotype

(Suyama et al., 2009)

involved_in regulation of myofibril size

inferred from mutant phenotype

(Fernandes and Schöck, 2014)

involved_in sarcomere organization

inferred from mutant phenotype

(Fernandes and Schöck, 2014)

involved_in sperm individualization

inferred from mutant phenotype

(Lee et al., 2008)

involved_in spermatogenesis

inferred from mutant phenotype

(Lee et al., 2008)

Terms Based on Predictions or Assertions (0 terms)

Cellular Component (5 terms)

Terms Based on Experimental Evidence (5 terms)

CV Term

Evidence

References

located_in A band

inferred from direct assay

(Fernandes and Schöck, 2014)

located_in cytosol

inferred from high throughput direct assay

(Lye et al., 2014)

located_in focal adhesion

inferred from direct assay

(Lee et al., 2008)

located_in sperm individualization complex

inferred from direct assay

(Lee et al., 2008)

located_in Z disc

inferred from direct assay

(Fernandes and Schöck, 2014)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

is_active_in focal adhesion

inferred from biological aspect of ancestor with PANTHER:PTN000867550

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Lasp for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q8I7C3)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.55

Gene model reviewed during 5.46

Low-frequency RNA-Seq exon junction(s) not annotated.

Annotated as dicistronic; possible double stop-codon readthrough.

Gene model reviewed during 6.05

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Lasp-RA

FBtr0075350

NM_140655

2793

504

Lasp-RB

FBtr0100145

NM_168681

4413

657

Lasp-RC

FBtr0306151

NM_001259869

3189

636

Lasp-RD

FBtr0306152

NM_001259870

2127

282

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

Lasp-PA

FBpp0075109

56.0

504

8.28

NP_648912

AAF49426

Lasp-PB

FBpp0099495

74.2

657

7.96

FBpp0297281

71.9

636

8.57

FBpp0297282

32.2

282

8.26

M9NFH3

NP_001246799

AFH04470

Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Subunit Structure (UniProtKB)

Interacts with osk.

(UniProt, Q8I7C3)

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Lasp using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Lasp_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

2.24

Transcript Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

organism | posterior

(Suyama et al., 2009)

(Lee et al., 2008)

(Lee et al., 2008)

(Lee et al., 2008)

individualization stage spermatid

(Lee et al., 2008)

male germline cell

(Lee et al., 2008)

oogenesis

germarium region 2

(Suyama et al., 2009)

germarium cap cell

(Suyama et al., 2009)

nurse cell

(Suyama et al., 2009)

nurse cell ring canal

(Suyama et al., 2009)

oocyte

(Suyama et al., 2009)

polar follicle cell

(Suyama et al., 2009)

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

day 7 of adulthood -- day 49 of adulthood

adult heart

(Cammarato et al., 2011)

Additional Descriptive Data

Lasp protein is detected in region 2 and in two cells (probably cap cells) at the anterior tip of the germarium. During mid-oogenesis, Lasp is abundant in nurse cells and in the oocyte. It is mildly enriched at the posterior end of the oocyte, is found in anterior and posterior polar follicle cells and is present on ring canals. At stage S10, it is detected at nurse cell borders, in ring canals and around the cortex of the oocyte. In preblastoderm embryos, it is observed at the posterior pole.

(Suyama et al., 2009)

Lasp is expressed in early germ cell but barely in later germ cells. A gradient is observed with the strongest expression in early germ cells. Lasp is also observed in hub cells and cyst cells. It is strongly localized to the actin cones of individualizing spermatids.

(Lee et al., 2008)

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in A band

inferred from direct assay

(Fernandes and Schöck, 2014)

located_in cytosol

inferred from high throughput direct assay

(Lye et al., 2014)

located_in focal adhesion

inferred from direct assay

(Lee et al., 2008)

located_in sperm individualization complex

inferred from direct assay

(Lee et al., 2008)

located_in Z disc

inferred from direct assay

(Fernandes and Schöck, 2014)

Expression Deduced from Reporters

Reporter: PBac{754.P.FSVS-0}Lasp^CPTI002905

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult brain

(Knowles-Barley, 2011.8.24)

adult gnathal ganglion | restricted

Comment: part of adult subesophageal ganglion

(Knowles-Barley, 2011.8.24)

lamina

(Knowles-Barley, 2011.8.24)

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Lasp in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Lasp

View exonic expression by tissue for Dmel\Lasp

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0063485

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

39864/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0063485

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0063485

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0063485

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG3849

Flygut - An atlas of the Drosophila adult midgut

FBgn0063485

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 24 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 17 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Lasp

Transgenic constructs containing regulatory region of Lasp

Aberrations (Deficiencies and Duplications) ( 2 )

Inferred from experimentation ( 2 )

Gene disrupted in

Df(3L)Exel6130

(Ryder, 2004.4.26)

Gene partially disrupted in

Df(3L)Lasp¹

(Lee et al., 2008)

Inferred from location ( 7 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal | recessive

Lasp^YC0059

lethal - all die before end of pupal stage, with Scer\GAL4^pnr-MD237

Lasp^GD10498

lethal - all die during P-stage, with Scer\GAL4^Mef2.PR

Lasp^GD10498

partially lethal - majority die

partially lethal - majority die, with Scer\GAL4^pnr-MD237

Lasp^GD16308

viable

Lasp¹

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

viable (with Df(3L)st7)

Lasp¹

Sterility

Allele

female semi-fertile

Lasp¹

grandchildless

Lasp^y45

male sterile (with Df(3L)st7)

Lasp¹

male sterile | recessive

Lasp¹

Other Phenotypes

Allele

abnormal flight | progressive

Lasp¹

abnormal hatching behavior

decreased cell number | embryonic stage 11

Lasp^y45

some die during pupal stage, with Scer\GAL4^pnr-MD237

visible, with Scer\GAL4^pnr-MD237

Lasp^GD16308

Phenotype manifest in

Allele

hub cell

Lasp¹

hub cell, with Scer\GAL4^C587

hub cell, with Scer\GAL4^ptc-559.1

Lasp^GD16308

I band | progressive

Lasp¹

individualization stage spermatid

Lasp¹

investment cone

Lasp¹

mesonotum, with Scer\GAL4^pnr-MD237

Lasp^GD16308

myofibril

Lasp¹

primordial germ cell | ectopic

Lasp^y41

primordial germ cell | embryonic stage 11

Lasp^y45

sarcomere

sarcomere (with Lasp¹)

Lasp^y41

sarcomere (with Lasp^y41)

Lasp¹

striated muscle thin filament

striated muscle thin filament (with Lasp¹)

Lasp^y41

striated muscle thin filament (with Lasp^y41)

Lasp¹

trichogen cell, with Scer\GAL4^pnr-MD237

Lasp^GD16308

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (41)

Hsap\LASP1

9 of 14

Yes

Hsap\NEBL

3 of 14

Yes

Hsap\NEB

2 of 14

Yes

Hsap\NRAP

2 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (26)

Rnor\Lasp1

9 of 14

Yes

Rnor\Nebl

2 of 14

Yes

Rnor\Neb

2 of 14

Yes

Rnor\Nrap

2 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Mus musculus (laboratory mouse) (23)

Mmus\Lasp1

9 of 14

Yes

Mmus\Neb

3 of 14

Yes

Mmus\Nrap

2 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Xenopus tropicalis (Western clawed frog) (18)

Xtro\lasp1

6 of 13

Yes

Xtro\nebl

2 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Xtro\mical3

1 of 13

Xtro\neb

1 of 13

Yes

Xtro\nrap

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Danio rerio (Zebrafish) (30)

Drer\lasp1

10 of 14

Yes

Drer\nebl

10 of 14

Yes

Drer\neb

3 of 14

Yes

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Drer\limd2

1 of 14

Yes

Drer\LOC556668

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Drer\si:ch211-51c14.1

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (16)

Cele\F42H10.3

9 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Anopheles gambiae (African malaria mosquito) (15)

Agam\AgaP_AGAP006526

10 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (6)

Atha\PLIM2a

2 of 13

Yes

Atha\PLIM2b

2 of 13

Yes

Atha\PLIM2c

2 of 13

Yes

Atha\WLIM1

2 of 13

Yes

Atha\WLIM2a

2 of 13

Yes

Atha\WLIM2b

2 of 13

Yes

Saccharomyces cerevisiae (Brewer's yeast) (2)

Scer\ABP1

1 of 13

Yes

Scer\NBP2

1 of 13

Yes

Schizosaccharomyces pombe (Fission yeast) (1)

Spom\cdc15

1 of 12

Yes

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Lasp. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (22)

Hil

2 of 13

Abi

1 of 13

Aplip1

1 of 13

Awh

1 of 13

CAP

1 of 13

CG31624

1 of 13

CG33521

1 of 13

cindr

1 of 13

Exn

1 of 13

Graf

1 of 13

jub

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Pax

1 of 13

POSH

1 of 13

Rassf

1 of 13

Zasp52

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

LASP1; LIM and SH3 protein 1

9 of 14

602920

NEBL; nebulette

3 of 14

605491

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

RNA-protein

Physical Interaction

Assay

References

Lasp - miso

anti tag coimmunoprecipitation, reverse transcription pcr

(Li et al., 2020)

protein-protein

Physical Interaction

Assay

References

Lasp - Actin

anti tag coimmunoprecipitation, peptide massfingerprinting, tandem affinity purification, western blot

(Fernandes and Schöck, 2014)

Lasp - Actn

tandem affinity purification, western blot, anti tag coimmunoprecipitation, peptide massfingerprinting

(Fernandes and Schöck, 2014)

Lasp - CAP

two hybrid

(Hu et al., 2017.6.13)

Lasp - Mhc

cosedimentation, molecular weight estimation by staining, anti tag coimmunoprecipitation, western blot, peptide massfingerprinting

(Fernandes and Schöck, 2014)

Lasp - Rsph3

two hybrid

(Hu et al., 2017.6.13)

Lasp - Strn-Mlck

anti tag coimmunoprecipitation, peptide massfingerprinting

(Fernandes and Schöck, 2014)

Lasp - brm

two hybrid

(Hu et al., 2017.6.13)

Lasp - bt

anti tag coimmunoprecipitation, peptide massfingerprinting

(Fernandes and Schöck, 2014)

Lasp - ft

anti tag coimmunoprecipitation, peptide massfingerprinting

(Kwon et al., 2013)

Lasp - hale

two hybrid

(Hu et al., 2017.6.13)

Lasp - l(3)05822

two hybrid

(Hu et al., 2017.6.13)

Lasp - mri

two hybrid

(Hu et al., 2017.6.13)

Lasp - osk

pull down, autoradiography, two hybrid

(Hu et al., 2017.6.13, Suyama et al., 2009)

Lasp - sls

anti tag coimmunoprecipitation, peptide massfingerprinting

(Fernandes and Schöck, 2014)

Lasp - up

anti tag coimmunoprecipitation, peptide massfingerprinting

(Fernandes and Schöck, 2014)

Lasp - wupA

anti tag coimmunoprecipitation, peptide massfingerprinting

(Fernandes and Schöck, 2014)

Lasp - yki

anti tag coimmunoprecipitation, peptide massfingerprinting

(Kwon et al., 2013)

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Lasp

enhanceable

osk

(Suyama et al., 2009)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

enhanceable

(Lee et al., 2008)

enhanceable

(Suyama et al., 2009)

osk

suppressible

Lasp

(Suyama et al., 2009)

External Data

Subunit Structure (UniProtKB)

Interacts with osk.

(UniProt, Q8I7C3 )

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0063485

MIST (genetic) - An integrated Molecular Interaction Database

39864

MIST (protein-protein) - An integrated Molecular Interaction Database

39864

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

SignaLink - A signaling pathway resource with multi-layered regulatory networks.

Q8I7C3

Class of Gene

gene_with_dicistronic_mRNA

(FlyBase Genome Annotators, 2012)

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-44

Cytogenetic map

73B6-73C1

Sequence location

3L:16,676,406..16,711,677 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

73B6-73C1

Limits computationally determined from genome sequence between P{PZ}Baldspot⁰²²⁸¹ and P{PZ}blot⁰¹⁶⁵⁸

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

3-44

(FlyBase, 2016)

3-39.3

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (15)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

85254

w¹¹¹⁸; PBac{WH}Lasp^f06993

26305

y¹ v¹; P{TRiP.JF02075}attP2

36169

y¹ w^*; Mi{MIC}Lasp^MI02943

15605

y¹ w^67c23; P{EPgy2}Lasp^EY02840

23528

w¹¹¹⁸; Mi{ET1}Lasp^MB03281 CG9692^MB03281

14911

y¹; P{SUPor-P}Lasp^KG06720 ry⁵⁰⁶/TM3, Sb¹ Ser¹

Kyoto

115582

w¹¹¹⁸; PBac{768.FSVS-0}Lasp^CPTI003412

115382

w¹¹¹⁸; PBac{754.P.FSVS-0}Lasp^CPTI002905

103545

y^* w^*; P{GawB}Lasp^NP0163 / TM6, P{UAS-lacZ.UW23-1}UW23-1

VDRC

v109416

P{KK108117}VIE-260B

v47126

w¹¹¹⁸ P{GD16308}v47126

v47127

w¹¹¹⁸ P{GD16308}v47127

More stocks available...

Genomic Clones (16)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (270)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

AT23571

Other clones

DPiM_AT23571

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0063485

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0063485

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Fernandes and Schöck, 2014, Suyama et al., 2009)

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

osk protein is absent from the posterior pole in Lasp mutant oocytes.

(Curado et al., 2001)

Transcription unit identified during molecular analysis of the Dab region.

(Gertler et al., 1993)

Relationship to Other Genes

Source for database merge of

Source for merge of: anon-73Bd Lasp

(FlyBase, 1996-)

Source for merge of: Lasp CG3849

(FlyBase, 1996-)

Additional comments

One or more of the processed transcripts for this gene contain(s) two non-overlapping open reading frames (ORFs). The non-overlapping ORFs are represented by CG43954 and Lasp.

(FlyBase Genome Annotators, 2012)

Source for merge of anon-73Bd Lasp was comparison of molecular map data with genome ( date:031008 ).

(FlyBase, 1996-)

Source for merge of Lasp CG3849 was sequence comparison ( date:030120 ).

(FlyBase, 1996-)

Nomenclature History

Source for database identify of

Nomenclature comments

Etymology

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

CG3849

(Harris et al., 2016, Fukuoh et al., 2014, Cammarato et al., 2011, Keleman et al., 2009.8.5, Perkins et al., 2009.3.31, Suyama et al., 2009, Lee et al., 2008, Quinones-Coello, 2007, Quinones-Coello, 2007, Driscoll and Gerstbrein, 2003)

anon-73Bd

cDNA B

(Gertler et al., 1993)

lasp

(Deng et al., 2021, Suyama et al., 2009)

Name Synonyms

Lasp

(Koehler and Huber, 2023, Reid et al., 2012, Cammarato et al., 2011, Chang et al., 2008)

Secondary FlyBase IDs

FBgn0016776
FBgn0036653

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 57 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

39864

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q8I7C3

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

Q8I7C3

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q8I7C3

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0063485

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

39864/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0063485

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0063485

FlyMine - An integrated database for Drosophila genomics

FBgn0063485

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG3849

MARRVEL_MODEL - MARRVEL (model organism gene)

39864

Linkouts

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0063485

DroID - A comprehensive database of gene and protein interactions.

FBgn0063485

DRSC - Results frm RNAi screens

FBgn0063485

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Lasp_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0063485

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0063485

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG3849

Flygut - An atlas of the Drosophila adult midgut

FBgn0063485

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC009504

MIST (genetic) - An integrated Molecular Interaction Database

39864

MIST (protein-protein) - An integrated Molecular Interaction Database

39864

SignaLink - A signaling pathway resource with multi-layered regulatory networks.

Q8I7C3

References (85)

The following external sites may use different assemblies or annotations than FlyBase.

2.24

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