FlyBase Gene Report: Dmel\Not1

General Information

Symbol

Dmel\Not1

Species

D. melanogaster

Name

Not1

Annotation Symbol

CG34407

Feature Type

protein_coding_gene

FlyBase ID

FBgn0085436

Gene Model Status

Current

Stock Availability

14 publicly available

Gene Summary

Not1 (Not1) encodes a poly(A)-specific ribonuclease involved in translation inhibition and ovarian follicle cell development. [Date last reviewed: 2019-08-01] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
Interactive Fly

Key Links

Genomic Location

Cytogenetic map

45F1-45F4

Sequence location

2R:9,566,146..9,579,007 [-]

Recombination map

2-61

RefSeq locus

NT_033778 REGION:9566146..9579007

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (12 terms)

Molecular Function (2 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

enables protein binding

inferred from physical interaction with UniProtKB:Q8SY33

(Braun et al., 2011)

inferred from physical interaction with UniProtKB:Q7KQM6

(Ruscica et al., 2019)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

enables molecular adaptor activity

inferred from biological aspect of ancestor with PANTHER:PTN000322838

(GO Reference Genome Project, 2011-)

Biological Process (6 terms)

Terms Based on Experimental Evidence (6 terms)

CV Term

Evidence

References

involved_in follicle cell of egg chamber development

inferred from mutant phenotype

(Jia et al., 2015)

involved_in mRNA catabolic process

inferred from direct assay

(Bawankar et al., 2013)

involved_in negative regulation of translation

inferred from direct assay

(Bawankar et al., 2013)

involved_in nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay

inferred from mutant phenotype

(Arvola et al., 2020)

involved_in nuclear-transcribed mRNA poly(A) tail shortening

inferred from mutant phenotype

(Temme et al., 2010)

involved_in P-body assembly

inferred from mutant phenotype

(Eulalio et al., 2007)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

involved_in negative regulation of translation

inferred from electronic annotation with InterPro:IPR040398

(InterPro Project Members, 2004-)

involved_in nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay

inferred from biological aspect of ancestor with PANTHER:PTN000322838

(GO Reference Genome Project, 2011-)

Cellular Component (4 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

part_of CCR4-NOT complex

inferred from physical interaction with FLYBASE:Marf1; FB:FBgn0039972

(Zhu et al., 2018)

inferred from direct assay

(Temme et al., 2010)

located_in cytosol

inferred from direct assay

(Temme et al., 2010)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

part_of CCR4-NOT core complex

inferred from biological aspect of ancestor with PANTHER:PTN000322838

(GO Reference Genome Project, 2011-)

inferred from electronic annotation with InterPro:IPR040398

(InterPro Project Members, 2004-)

is_active_in P-body

inferred from biological aspect of ancestor with PANTHER:PTN000322838

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

CCR4-NOT COMPLEX

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

Not1 (Not1) encodes a poly(A)-specific ribonuclease involved in translation inhibition and ovarian follicle cell development. [Date last reviewed: 2019-08-01]

Gene Group (FlyBase)

CCR4-NOT COMPLEX -: The CCR4-NOT complex is a conserved complex implicated in various cellular processes to regulate gene expression. It is best characterised in its role as the main enzyme responsible for the deadenylation of mRNA. The conserved core of the complex is composed of two modules: the NOT module and a catalytic module comprising two deadenylases, twin and Pop2. (Adapted from FBrf0221179 and FBrf0231273).

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Not1 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry A0A0B4LEZ3)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.46

Gene model reviewed during 5.49

Tissue-specific extension of 3' UTRs observed during later stages (FBrf0218523, FBrf0219848); all variants may not be annotated

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Not1-RC

FBtr0112672

NM_001103772

8700

2505

Not1-RD

FBtr0112673

NM_001103773

8694

2503

Not1-RE

FBtr0112674

NM_001103774

8201

2220

Not1-RF

FBtr0302122

NM_001169628

8340

2492

Not1-RG

FBtr0304957

NM_165678

10068

2505

Not1-RH

FBtr0339394

NM_001299323

8191

2511

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

Not1-PC

FBpp0111584

281.1

2505

6.74

FBpp0111585

280.9

2503

6.72

FBpp0111586

249.8

2220

6.95

FBpp0291332

279.9

2492

6.80

FBpp0293496

281.1

2505

6.74

FBpp0308486

281.7

2511

6.72

A0A0B4LEZ3

NP_001286252

AHN56050

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

2505 aa isoforms: Not1-PC, Not1-PG

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Not1 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.18

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 7 -- 8

anterior endoderm anlage

(Fisher et al., 2012)

head mesoderm

(Fisher et al., 2012)

hindgut anlage

(Fisher et al., 2012)

organism | ubiquitous

(Fisher et al., 2012)

posterior endoderm

(Fisher et al., 2012)

trunk mesoderm

(Fisher et al., 2012)

embryonic stage 9 -- 10

anterior endoderm

(Fisher et al., 2012)

inclusive hindgut primordium

(Fisher et al., 2012)

organism | ubiquitous

(Fisher et al., 2012)

posterior endoderm

(Fisher et al., 2012)

trunk mesoderm

(Fisher et al., 2012)

ventral nerve cord primordium

(Fisher et al., 2012)

embryonic stage 13 -- 16

embryonic brain

(Fisher et al., 2012)

embryonic/larval midgut

(Fisher et al., 2012)

larval nervous system

(Fisher et al., 2012)

gonad

(Fisher et al., 2012)

organism | ubiquitous

(Fisher et al., 2012)

larval ventral nerve cord

(Fisher et al., 2012)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

day 5 of adulthood | male -- day 7 of adulthood | male

spermatozoon

(Wasbrough et al., 2010)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

part_of CCR4-NOT complex

inferred from physical interaction with FLYBASE:Marf1; FB:FBgn0039972

(Zhu et al., 2018)

inferred from direct assay

(Temme et al., 2010)

located_in cytosol

inferred from direct assay

(Temme et al., 2010)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Not1 in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Not1

View exonic expression by tissue for Dmel\Not1

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0085436

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

5740252/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0085436

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0085436

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0085436

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG1884

Flygut - An atlas of the Drosophila adult midgut

FBgn0085436

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\Not1

Stages(s) 1-3
Stages(s) 4-6
Stages(s) 9-10
Stages(s) 11-12
Stages(s) 13-16

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 6 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 11 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Not1

Transgenic constructs containing regulatory region of Not1

Aberrations (Deficiencies and Duplications) ( 1 )

Inferred from experimentation ( 1 )

Gene disrupted in

Df(2R)Exel8049

(Ryder, 2004.4.26)

Inferred from location ( 3 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Not1^GD9640

lethal, with Scer\GAL4^insc-Mz1407

Not1^GD4070

lethal, with Scer\GAL4^Mef2.PR

Not1^GD9640

lethal, with Scer\GAL4^pnr-MD237

lethal | pupal stage, with Scer\GAL4^pnr-MD237

Not1^GD9640

lethal - all die during embryonic stage, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Not1^GD9640

lethal - all die during larval stage, with Scer\GAL4^Mef2.PR

Not1^GD4070

partially lethal, with Scer\GAL4^tin.CΔ4

Not1^GD4070

Other Phenotypes

Allele

abnormal developmental rate, with Scer\GAL4^elav-C155

abnormal locomotor behavior | adult stage | RU486 conditional, with Scer\GAL4^{elav.Switch.PO}

abnormal locomotor rhythm | adult stage, with Scer\GAL4^P2.4.Pdf

Not1^JF03096

abnormal memory | adult stage | RU486 conditional, with Scer\GAL4^{elav.Switch.PO}

abnormal memory | third instar larval stage, with Scer\GAL4^ey-OK107

abnormal memory | third instar larval stage | RU486 conditional, with Scer\GAL4^{elav.Switch.PO}

abnormal neuroanatomy | larval stage, with Scer\GAL4^insc-Mz1407

Not1^GD4070

neoplasia | adult stage | male, with Scer\GAL4^VP16.bam

short lived | heat sensitive, with Scer\GAL4^HCH.Hand

Not1^GD4070

short lived | heat sensitive, with Scer\GAL4^tin.CΔ4

Not1^GD4070

visible, with Scer\GAL4^Bx-MS1096

visible, with Scer\GAL4^ey.PU

Not1^RNAi.UAS.WIZ

Phenotype manifest in

Allele

dorsal appendage, with Scer\GAL4^GR1

Not1^HMS00526

egg chamber, with Scer\GAL4^GR1

Not1^HMS00526

eye, with Scer\GAL4^ey.PU

Not1^RNAi.UAS.WIZ

ganglion mother cell | larval stage, with Scer\GAL4^insc-Mz1407

Not1^GD4070

larval neuroblast | larval stage, with Scer\GAL4^insc-Mz1407

Not1^GD4070

larval somatic muscle cell, with Scer\GAL4^Mef2.PR

Not1^GD4070

sarcomere, with Scer\GAL4^Mef2.PR

Not1^GD4070

spermatogonium, with Scer\GAL4^VP16.bam

testis, with Scer\GAL4^VP16.bam

wing, with Scer\GAL4^Bx-MS1096

Not1^RNAi.UAS.WIZ

wing vein | ectopic, with Scer\GAL4^Bx-MS1096

Not1^UAS.Tag:HA

Z disc, with Scer\GAL4^Mef2.PR

Not1^GD4070

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (1)

Hsap\CNOT1

14 of 14

Yes

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (1)

Rnor\Cnot1

14 of 14

Yes

Mus musculus (laboratory mouse) (1)

Mmus\Cnot1

13 of 14

Yes

Xenopus tropicalis (Western clawed frog) (2)

Xtro\cnot1

11 of 13

Yes

Xtro\mapkap1

1 of 13

Danio rerio (Zebrafish) (1)

Drer\cnot1

13 of 14

Yes

Caenorhabditis elegans (Nematode, roundworm) (1)

Cele\let-711

14 of 14

Yes

Anopheles gambiae (African malaria mosquito) (1)

Agam\AgaP_AGAP009057

12 of 12

Yes

Arabidopsis thaliana (thale-cress) (1)

Atha\AT1G02080

12 of 13

Yes

Saccharomyces cerevisiae (Brewer's yeast) (1)

Scer\CDC39

11 of 13

Yes

Schizosaccharomyces pombe (Fission yeast) (1)

Spom\not1

10 of 12

Yes

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Not1. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (0)

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 3 )

Allele

Disease

Evidence

References

Not1^GD4070

model of heart disease

CEA

(Elmén et al., 2020)

model of Vissers-Bodmer syndrome

CEA

(Vissers et al., 2020)

Not1^GD9640

model of cancer

CEA with Delta^UAS.cDa

(Vicente et al., 2018)

Not1^KK100090

model of Vissers-Bodmer syndrome

CEA

(Vissers et al., 2020)

Potential Models Based on Orthology ( 2 )

Human Ortholog

Disease

Evidence

References

CNOT1; CCR4-NOT transcription complex subunit 1

model of holoprosencephaly 12

IEA

(FlyBase, 2019-)

model of Vissers-Bodmer syndrome

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 5 )

Allele

Disease

Interaction

References

Not1^HMS00526

exacerbates tauopathy

modeled by Hsap\MAPT^UAS.cWa

(Feuillette et al., 2020)

exacerbates cancer

modeled by Rbf^HMS03004

(Parkhitko et al., 2021)

Not1^JF01135

exacerbates tauopathy

modeled by Hsap\MAPT^UAS.cWa

(Feuillette et al., 2020)

Not1^GD4070

model of Vissers-Bodmer syndrome

is ameliorated by E(z)^RNAi.UAS.cUa

(Vissers et al., 2020)

is exacerbated by Prosap^GD10101

(Vissers et al., 2020)

is exacerbated by Prosap^KK101537

(Vissers et al., 2020)

is exacerbated by ash1^KK100243

(Vissers et al., 2020)

is ameliorated by esc^RNAi.UAS.cUa

(Vissers et al., 2020)

is exacerbated by mnb^GL00104

(Vissers et al., 2020)

is exacerbated by mnb^KK102642

(Vissers et al., 2020)

is exacerbated by skd^HMS01305

(Vissers et al., 2020)

is ameliorated by skd^UAS.cJa

(Vissers et al., 2020)

is exacerbated by skd^VSH330726

(Vissers et al., 2020)

is ameliorated by Hsap\CNOT1^UAS.cVa

(Vissers et al., 2020)

is ameliorated by E(z)^RNAi.UAS.cUa

(Vissers et al., 2020)

Not1^KK100090

model of Vissers-Bodmer syndrome

is ameliorated by Hsap\CNOT1^UAS.cVa

(Vissers et al., 2020)

Not1^JF03096

exacerbates tauopathy

modeled by Hsap\MAPT^UAS.cWa

(Feuillette et al., 2020)

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

CNOT1; CCR4-NOT transcription complex subunit 1

14 of 14

CCR4-NOT TRANSCRIPTION COMPLEX, SUBUNIT 1; CNOT1

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Dmel gene

Ortholog showing functional complementation

Supporting References

Not1

Hsap\CNOT1

(Vissers et al., 2020)

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

RNA-protein

Physical Interaction

Assay

References

Not1 - bel

clip-seq

(Kotov et al., 2020)

protein-protein

Physical Interaction

Assay

References

Not1 - Atx2

anti bait coimmunoprecipitation, western blot

(Lee et al., 2017)

Not1 - Gyf

anti tag coimmunoprecipitation, anti tag western blot

(Ruscica et al., 2019)

Not1 - Helz

anti tag coimmunoprecipitation, anti tag western blot

(Hanet et al., 2019)

Not1 - Marf

colocalization, fluorescence microscopy, inferred by author

(Salgania et al., 2024)

Not1 - Marf1

anti tag coimmunoprecipitation, western blot

(Zhu et al., 2018)

Not1 - Not10

anti tag coimmunoprecipitation, anti tag western blot

(Bawankar et al., 2013)

Not1 - Not11

anti tag coimmunoprecipitation, anti tag western blot, pull down, molecular weight estimation by staining

(Haugen et al., 2022, Bawankar et al., 2013)

Not1 - Not3

anti tag coimmunoprecipitation, anti tag western blot, molecular sieving, molecular weight estimation by staining, colocalization, fluorescence microscopy, inferred by author, experimental knowledge based, anti bait coimmunoprecipitation, Identification by mass spectrometry, western blot

(Pekovic and Wahle, 2024, Salgania et al., 2024, Arvola et al., 2020, Bawankar et al., 2013, Guruharsha et al., 2011, Temme et al., 2010)

Not1 - Pop2

experimental knowledge based, anti tag coimmunoprecipitation, anti tag western blot, molecular sieving, molecular weight estimation by staining, colocalization, fluorescence microscopy, inferred by author, anti bait coimmunoprecipitation, Identification by mass spectrometry, western blot

(Pekovic and Wahle, 2024, Salgania et al., 2024, Bawankar et al., 2013, Petit et al., 2012, Guruharsha et al., 2011, Temme et al., 2010)

Not1 - Pos

anti tag coimmunoprecipitation, anti tag western blot

(Xia et al., 2022)

Not1 - Rcd-1

colocalization, fluorescence microscopy, inferred by author, anti tag coimmunoprecipitation, anti tag western blot, molecular sieving, molecular weight estimation by staining

(Pekovic and Wahle, 2024, Salgania et al., 2024, Xia et al., 2022, Bawankar et al., 2013)

Not1 - Rga

anti tag coimmunoprecipitation, anti tag western blot, experimental knowledge based, molecular sieving, molecular weight estimation by staining, western blot, anti bait coimmunoprecipitation, colocalization, fluorescence microscopy, inferred by author, Identification by mass spectrometry

(Pekovic and Wahle, 2024, Salgania et al., 2024, Arvola et al., 2020, Bawankar et al., 2013, Guruharsha et al., 2011, Temme et al., 2010)

Not1 - RpL10Ab

pull down, western blot

(Antic et al., 2015)

Not1 - aub

anti tag coimmunoprecipitation, western blot

(Rojas-Ríos et al., 2017)

Not1 - bam

anti tag coimmunoprecipitation, anti tag western blot, pull down, molecular weight estimation by staining

(Sgromo et al., 2018)

Not1 - cu

anti bait coimmunoprecipitation, anti tag western blot

(Temme et al., 2010)

Not1 - cup

anti tag coimmunoprecipitation, anti tag western blot

(Igreja and Izaurralde, 2011)

Not1 - gw

anti bait coimmunoprecipitation, western blot, anti tag coimmunoprecipitation, anti tag western blot

(Huntzinger et al., 2013, Braun et al., 2011)

Not1 - me31B

anti tag coimmunoprecipitation, western blot

(Lee et al., 2017)

Not1 - nanos

colocalization, fluorescence microscopy, inferred by author, pull down, molecular weight estimation by staining, anti tag coimmunoprecipitation, anti tag western blot, western blot

(Salgania et al., 2024, Arvola et al., 2020, Raisch et al., 2016)

Not1 - piwi

anti bait coimmunoprecipitation, western blot

(Kordyukova et al., 2020)

Not1 - pum

two hybrid, anti tag coimmunoprecipitation, anti tag western blot, western blot

(Haugen et al., 2022, Arvola et al., 2020)

Not1 - rictor

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Not1 - roq

colocalization, fluorescence microscopy, inferred by author, anti tag coimmunoprecipitation, anti tag western blot

(Salgania et al., 2024, Sgromo et al., 2017)

Not1 - smg

tandem affinity purification, Identification by mass spectrometry, two hybrid

(Pekovic et al., 2023, Semotok et al., 2005)

Not1 - thoc5

anti bait coimmunoprecipitation, western blot

(Kordyukova et al., 2020)

Not1 - twin

anti bait coimmunoprecipitation, western blot, experimental knowledge based, anti tag coimmunoprecipitation, Identification by mass spectrometry, molecular sieving, molecular weight estimation by staining, anti tag western blot

(Pekovic and Wahle, 2024, Kordyukova et al., 2020, Morgunova et al., 2015, Bawankar et al., 2013, Joly et al., 2013, Guruharsha et al., 2011, Temme et al., 2010)

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Not1

enhanceable

ash1

(Vissers et al., 2020)

enhanceable

(Lee et al., 2017)

suppressible

(Vissers et al., 2020)

Not1

suppressible

esc

(Vissers et al., 2020)

Not1

enhanceable

mnb

(Vissers et al., 2020)

Not1

enhanceable

Prosap

(Vissers et al., 2020)

Not1

suppressible

skd

(Vissers et al., 2020)

Not1

enhanceable

skd

(Vissers et al., 2020)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

brat

enhanceable

Not1

(Komori et al., 2018)

Delta

enhanceable

Not1

(Vicente et al., 2018)

tut

enhanceable

Not1

(Shan et al., 2017)

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

624883

DroID - A comprehensive database of gene and protein interactions.

FBgn0085436

MIST (protein-protein) - An integrated Molecular Interaction Database

5740252

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

RNA degradation

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-61

Cytogenetic map

45F1-45F4

Sequence location

2R:9,566,146..9,579,007 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

45F1-45F4

Limits computationally determined from genome sequence between P{EP}EP2165&P{lacW}l(2)k01301^k01301 and P{lacW}l(2)k09501^k09501

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

2-61

(FlyBase, 2016)

2-59.4

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (14)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

28681

y¹ v¹; P{TRiP.JF03096}attP2

31696

y¹ v¹; P{TRiP.JF01135}attP2

44201

y¹ w^*; Mi{MIC}Not1^MI07631/SM6a

27186

y¹ w^*; P{EP}Not1^G5743 Nt5c^G5743

62592

w¹¹¹⁸; PBac{IT.GAL4}Not1^0042-G4 Nt5c^0042-G4

76690

y¹ w^*; Mi{Trojan-GAL4.0}Not1^{MI07631-TG4.0}/SM6a

80000

y¹ sc^* v¹ sev²¹; P{TOE.GS02841}attP40

Kyoto

123349

w¹¹¹⁸; P{RS3}Not1^CB-0865-3

1884R-1

1884R-3

v106587

v45463

w¹¹¹⁸; P{GD9640}v45463/CyO

More stocks available...

Genomic Clones (14)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (78)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

MIP03068

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0085436

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0085436

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Temme et al., 2010)

monoclonal

(Temme et al., 2010)

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

dsRNA made from templates generated with primers directed against this gene are used to study its function.

(Behm-Ansmant et al., 2006)

dsRNA has been made from templates generated with primers directed against this gene. RNAi of Not1 results in dorsal overextension of primary dendrites and a reduction in lateral branching. RNAi also causes defects in muscle and defects in dendrite morphogenesis.

(Parrish et al., 2006)

Identified as a potential component of the hh signalling pathway in a genome-wide RNAi screen. dsRNA made from templates generated with primers directed affects the extent of expression of a hh signaling reporter construct in Clone 8 cells.

(Nybakken et al., 2005)

Area matching Drosophila EST AA699194.

(Davies, 2001.3.15)

Relationship to Other Genes

Source for database merge of

Source for merge of: Not1 CG1874

(FlyBase Genome Annotators, 2007)

Source for merge of: CG1884 anon- EST:Liang-1.29

(FlyBase, 1996-)

Source for merge of: CG1884 anon-WO0118547.221

(FlyBase, 1996-)

Additional comments

Annotations CG1884 and CG1874 merged as CG34407 in release 5.2 of the genome annotation.

(FlyBase Genome Annotators, 2007)

Source for merge of CG1884 anon- EST:Liang-1.29 was sequence comparison ( date:030722 ).

(FlyBase, 1996-)

Source for merge of CG1884 anon-WO0118547.221 was sequence comparison ( date:051113 ).

(FlyBase, 1996-)

Nomenclature History

Source for database identify of

Source for identity of: CG1884 Not1

(Semotok et al., 2005)

Nomenclature comments

Etymology

Synonyms and Secondary IDs (10)

Reported As

Symbol Synonym

CG1874

(Fukuoh et al., 2014, Zhou et al., 2008, Nybakken et al., 2005)

CG1884

(Lin et al., 2015, Schertel et al., 2013, Christis and Munro, 2012, Yano et al., 2012, Müller et al., 2010, Perkins et al., 2009, Bjorklund et al., 2006, Parrish et al., 2006, Yandell et al., 2005, Davies et al., 2001, FlyBase, 1996-)

CG34407

(Kolkhof et al., 2017, Çiçek et al., 2016, Hosono et al., 2015, Ni et al., 2010.12.1, Keleman et al., 2009.8.5, Ni et al., 2008, FlyBase Genome Annotators, 2007)

NOT

(Seydoux and Braun, 2006)

Not1

(Bandyadka et al., 2025, Kagemann et al., 2025, Salgania et al., 2024, Beaver et al., 2022, Haugen et al., 2022, Connacher and Goldstrohm, 2021, Khan et al., 2021, Parkhitko et al., 2021, Wang and Lin, 2021, Arvola et al., 2020, Elmén et al., 2020, Feuillette et al., 2020, Kordyukova et al., 2020, Vissers et al., 2020, Foley et al., 2019, Grima et al., 2019, Jia et al., 2019, Greenblatt and Spradling, 2018, Zeng et al., 2018, Zhu et al., 2018, Lee et al., 2017, Shan et al., 2017, Transgenic RNAi Project members, 2017-, Brewer-Jensen et al., 2016, Clandinin and Owens, 2016-, Gene Disruption Project members, 2016-, Patel et al., 2016, Jia et al., 2015, Okada et al., 2015, Choi et al., 2014, Temme et al., 2014, Japanese National Institute of Genetics, 2012.5.21, Spasic et al., 2012, Yano et al., 2012, Friedman et al., 2011, Neumüller et al., 2011, Temme et al., 2010, Wasbrough et al., 2010)

anon-EST:Liang-1.29

anon-WO0118547.221

clone 1.29

(Liang and Biggin, 1998)

not1

(Kotov et al., 2020, Flora et al., 2018, Komori et al., 2018, Fu et al., 2015, Neely et al., 2010)

Name Synonyms

CG1874

Not1

Secondary FlyBase IDs

FBgn0033424
FBgn0033425
FBgn0026000
FBgn0062162

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 73 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

5740252

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

A0A0B4LEZ3

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

A0A0B4LEZ3

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0085436

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

5740252/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0085436

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0085436

FlyMine - An integrated database for Drosophila genomics

FBgn0085436

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG34407

MARRVEL_MODEL - MARRVEL (model organism gene)

5740252

Linkouts

BioGRID - A database of protein and genetic interactions.

624883

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0085436

DroID - A comprehensive database of gene and protein interactions.

FBgn0085436

DRSC - Results frm RNAi screens

FBgn0085436

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0085436

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0085436

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG1884

Flygut - An atlas of the Drosophila adult midgut

FBgn0085436

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC003500

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

RNA degradation

MIST (protein-protein) - An integrated Molecular Interaction Database

5740252

References (139)

The following external sites may use different assemblies or annotations than FlyBase.

-0.18

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