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General Information
Symbol
Dmel\tum
Species
D. melanogaster
Name
tumbleweed
Annotation Symbol
CG13345
Feature Type
FlyBase ID
FBgn0086356
Gene Model Status
Stock Availability
Gene Summary
tumbleweed (tum) encodes a GTPase activating protein for Rho family GTPases involved in Wnt signalling regulation. [Date last reviewed: 2019-09-12] (FlyBase Gene Snapshot)
Also Known As

RacGAP50C, RacGAP, acGAP, DRacGAP

Key Links
Genomic Location
Cytogenetic map
Sequence location
2R:13,856,150..13,858,689 [-]
Recombination map
2-68
RefSeq locus
NT_033778 REGION:13856150..13858689
Sequence
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
Function
GO Summary Ribbons
Gene Ontology (GO) Annotations (22 terms)
Molecular Function (1 term)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
inferred from sequence or structural similarity with SGD:S000000464
Biological Process (15 terms)
Terms Based on Experimental Evidence (15 terms)
CV Term
Evidence
References
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from genetic interaction with FLYBASE:nkd; FB:FBgn0002945
inferred from genetic interaction with FLYBASE:Axn; FB:FBgn0026597
inferred from genetic interaction with FLYBASE:arm; FB:FBgn0000117
inferred from genetic interaction with FLYBASE:pav; FB:FBgn0011692
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from genetic interaction with FLYBASE:vn; FB:FBgn0003984
inferred from genetic interaction with FLYBASE:aos; FB:FBgn0004569
inferred from genetic interaction with FLYBASE:rho; FB:FBgn0004635
inferred from genetic interaction with FLYBASE:Pak; FB:FBgn0267698
inferred from mutant phenotype
involved_in wound healing
inferred from mutant phenotype
Terms Based on Predictions or Assertions (0 terms)
Cellular Component (6 terms)
Terms Based on Experimental Evidence (6 terms)
CV Term
Evidence
References
inferred from direct assay
colocalizes_with cortical microtubule
inferred from direct assay
colocalizes_with midbody
inferred from direct assay
located_in nucleus
inferred from direct assay
inferred from direct assay
colocalizes_with spindle
inferred from direct assay
Terms Based on Predictions or Assertions (0 terms)
Gene Group (FlyBase)
Protein Family (UniProt)
-
Summaries
Gene Snapshot
tumbleweed (tum) encodes a GTPase activating protein for Rho family GTPases involved in Wnt signalling regulation. [Date last reviewed: 2019-09-12]
Gene Group (FlyBase)
RHO GTPASE ACTIVATING PROTEINS -
RhoGAPs are GTPase activating proteins for Rho family GTPases. (Adapted from PMID:15731001 and FBrf0158741).
Pathway (FlyBase)
Negative Regulators of Wnt-TCF Signaling Pathway -
Negative regulators of Wnt-TCF (canonical Wnt) signaling down-regulate the pathway, resulting in the attenuation of transcriptional regulation mediated by β-catenin (arm).
Negative Regulators of EGFR Signaling Pathway -
Negative regulators of Epidermal Growth Factor Receptor signaling down-regulate the pathway, suppressing the activation of ERK kinase (rl) or acting on downstream effectors.
Summary (Interactive Fly)

cytoskeletal regulator required for cytokinesis - connects the contractile ring to cortical microtubules at the site of furrowing in dividing cells -negatively regulates the wingless pathway during Drosophila embryonic development - required for neuroblast proliferation and limits axon growth

Gene Model and Products
Number of Transcripts
1
Number of Unique Polypeptides
1

Please see the JBrowse view of Dmel\tum for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model

Gene model reviewed during 5.50

Sequence Ontology: Class of Gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
FBtr0087641
2353
625
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
FBpp0086767
69.8
625
9.33
Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\tum using the Feature Mapper tool.

External Data
Crossreferences
Linkouts
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
cell
midbody

Comment: telophase; expression assayed in cultured S2 cells

cell
nucleus

Comment: interphase; expression assayed in cultured S2 cells

cell
spindle

Comment: metaphase; expression assayed in cultured S2 cells

cell

Comment: expression assayed in cultured S2 cells; anaphase

Additional Descriptive Data

tum protein is localized throughout muscle fibers and in discrete cytoplasmic puncta in body wall muscles in late embryos.

Marker for
 
Subcellular Localization
CV Term
Evidence
References
inferred from direct assay
colocalizes_with cortical microtubule
inferred from direct assay
colocalizes_with midbody
inferred from direct assay
located_in nucleus
inferred from direct assay
inferred from direct assay
colocalizes_with spindle
inferred from direct assay
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\tum in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
FLIGHT - Cell culture data for RNAi and other high-throughput technologies
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 9 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 33 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of tum
Transgenic constructs containing regulatory region of tum
Aberrations (Deficiencies and Duplications) ( 3 )
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
Other Phenotypes
Allele
Phenotype manifest in
Allele
embryonic central nervous system & neuron
embryonic epidermis & nucleus
eye & ommatidium, with Scer\GAL4ey.PB
mushroom body & axon | somatic clone
sensillum campaniformium & wing vein L3 | supernumerary, with Scer\GAL469B
sensillum campaniformium & wing vein L3 | supernumerary, with Scer\GAL4Bx-MS1096
sensillum campaniformium & wing vein L3 | supernumerary, with Scer\GAL4en-e16E
sensillum campaniformium & wing vein L4 | supernumerary, with Scer\GAL469B
sensillum campaniformium & wing vein L4 | supernumerary, with Scer\GAL4Bx-MS1096
sensillum campaniformium & wing vein L4 | supernumerary, with Scer\GAL4en-e16E
sensillum campaniformium & wing vein L5 | supernumerary, with Scer\GAL469B
sensillum campaniformium & wing vein L5 | supernumerary, with Scer\GAL4Bx-MS1096
sensillum campaniformium & wing vein L5 | supernumerary, with Scer\GAL4en-e16E
Orthologs
Human Orthologs (via DIOPT v8.0)
Homo sapiens (Human) (2)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
14 of 15
Yes
Yes
1 of 15
No
No
Model Organism Orthologs (via DIOPT v8.0)
Mus musculus (laboratory mouse) (2)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
13 of 15
Yes
Yes
1 of 15
No
No
Rattus norvegicus (Norway rat) (3)
10 of 13
Yes
Yes
2 of 13
No
Yes
1 of 13
No
No
Xenopus tropicalis (Western clawed frog) (3)
9 of 12
Yes
Yes
2 of 12
No
Yes
1 of 12
No
No
Danio rerio (Zebrafish) (3)
13 of 15
Yes
Yes
1 of 15
No
No
Caenorhabditis elegans (Nematode, roundworm) (1)
9 of 15
Yes
Yes
Arabidopsis thaliana (thale-cress) (0)
No records found.
Saccharomyces cerevisiae (Brewer's yeast) (1)
1 of 15
Yes
No
Schizosaccharomyces pombe (Fission yeast) (1)
1 of 12
Yes
No
Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG091904FL )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila suzukii
Spotted wing Drosophila
Drosophila simulans
Drosophila sechellia
Drosophila erecta
Drosophila yakuba
Drosophila ananassae
Drosophila pseudoobscura pseudoobscura
Drosophila persimilis
Drosophila willistoni
Drosophila virilis
Drosophila mojavensis
Drosophila grimshawi
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091503J7 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Glossina morsitans
Tsetse fly
Lucilia cuprina
Australian sheep blowfly
Mayetiola destructor
Hessian fly
Mayetiola destructor
Hessian fly
Aedes aegypti
Yellow fever mosquito
Anopheles darlingi
American malaria mosquito
Anopheles gambiae
Malaria mosquito
Culex quinquefasciatus
Southern house mosquito
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W04MY )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Danaus plexippus
Monarch butterfly
Heliconius melpomene
Postman butterfly
Apis florea
Little honeybee
Apis mellifera
Western honey bee
Bombus impatiens
Common eastern bumble bee
Bombus terrestris
Buff-tailed bumblebee
Linepithema humile
Argentine ant
Megachile rotundata
Alfalfa leafcutting bee
Nasonia vitripennis
Parasitic wasp
Dendroctonus ponderosae
Mountain pine beetle
Tribolium castaneum
Red flour beetle
Pediculus humanus
Human body louse
Pediculus humanus
Human body louse
Rhodnius prolixus
Kissing bug
Cimex lectularius
Bed bug
Acyrthosiphon pisum
Pea aphid
Acyrthosiphon pisum
Pea aphid
Acyrthosiphon pisum
Pea aphid
Acyrthosiphon pisum
Pea aphid
Acyrthosiphon pisum
Pea aphid
Zootermopsis nevadensis
Nevada dampwood termite
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X04JD )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strigamia maritima
European centipede
Ixodes scapularis
Black-legged tick
Ixodes scapularis
Black-legged tick
Stegodyphus mimosarum
African social velvet spider
Tetranychus urticae
Two-spotted spider mite
Tetranychus urticae
Two-spotted spider mite
Daphnia pulex
Water flea
Daphnia pulex
Water flea
Daphnia pulex
Water flea
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G03O7 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strongylocentrotus purpuratus
Purple sea urchin
Ciona intestinalis
Vase tunicate
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Paralogs
Paralogs (via DIOPT v8.0)
Drosophila melanogaster (Fruit fly) (3)
6 of 10
1 of 10
1 of 10
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Model Summary Ribbon
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 0 )
    Human Ortholog
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Interaction
    References
    Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    Functional Complementation Data
    Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
    Interactions
    Summary of Physical Interactions
    esyN Network Diagram
    Show neighbor-neighbor interactions:
    Select Layout:
    Legend:
    Protein
    RNA
    Selected Interactor(s)
    Interactions Browser

    Please see the Physical Interaction reports below for full details
    protein-protein
    Physical Interaction
    Assay
    References
    Summary of Genetic Interactions
    esyN Network Diagram
    esyN Network Key:
    Suppression
    Enhancement

    Please look at the allele data for full details of the genetic interactions
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    External Data
    Linkouts
    BioGRID - A database of protein and genetic interactions.
    DroID - A comprehensive database of gene and protein interactions.
    InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
    MIST (genetic) - An integrated Molecular Interaction Database
    MIST (protein-protein) - An integrated Molecular Interaction Database
    Pathways
    Signaling Pathways (FlyBase)
    Negative Regulators of Wnt-TCF Signaling Pathway -
    Negative regulators of Wnt-TCF (canonical Wnt) signaling down-regulate the pathway, resulting in the attenuation of transcriptional regulation mediated by β-catenin (arm).
    Negative Regulators of EGFR Signaling Pathway -
    Negative regulators of Epidermal Growth Factor Receptor signaling down-regulate the pathway, suppressing the activation of ERK kinase (rl) or acting on downstream effectors.
    Metabolic Pathways
    External Data
    Linkouts
    Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
    Genomic Location and Detailed Mapping Data
    Chromosome (arm)
    2R
    Recombination map
    2-68
    Cytogenetic map
    Sequence location
    2R:13,856,150..13,858,689 [-]
    FlyBase Computed Cytological Location
    Cytogenetic map
    Evidence for location
    50C6-50D
    Limits computationally determined from genome sequence between P{lacW}l(2)03105k16702&P{lacW}fl(2)dk16105 and P{lacW}shotk03010; Left limit from (method unavailable) (FBrf0112136) Right limit from (method unavailable) (FBrf0112136)
    Experimentally Determined Cytological Location
    Cytogenetic map
    Notes
    References
    50C4-50C8
    (determined by in situ hybridisation)
    Determined by deficiency mapping (details unspecified).
    Experimentally Determined Recombination Data
    Location
    Notes
    Stocks and Reagents
    Stocks (15)
    Genomic Clones (15)
     

    Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

    cDNA Clones (125)
     

    Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

    cDNA clones, fully sequenced
    BDGP DGC clones
    Other clones
    Drosophila Genomics Resource Center cDNA clones

    For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

    cDNA Clones, End Sequenced (ESTs)
    RNAi and Array Information
    Linkouts
    DRSC - Results frm RNAi screens
    GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
    Antibody Information
    Laboratory Generated Antibodies
     
    Commercially Available Antibodies
     
    Developmental Studies Hybridoma Bank - Monoclonal antibodies for use in research
    Other Information
    Relationship to Other Genes
    Source for database identify of

    Source for identity of: RacGAP50C eon

    Source for identity of: acGAP CG13345

    Source for database merge of

    Source for merge of: RacGAP50C tum

    Additional comments

    Source for identity of acGAP CG13345 was sequence comparison ( date:000721 ).

    Other Comments

    tum regulates microtubule organisation during myogenesis. tum is required for the change in localisation of the microtubule nucleator γ-tubulin, from a diffuse cytoplasmic location to discrete cytoplasmic puncta at the nuclear periphery, that normally occurs during myogenesis.

    RNAi screen using dsRNA made from templates generated with primers directed against this gene causes defects in spindle shape and cytokinesis failure when assayed in S2 cells. This phenotype can be observed when the screen is performed with or without Cdc27 dsRNA.

    dsRNA has been made from templates generated with primers directed against this gene. RNAi of tum results in supernumerary class I neurons and a range of arborization defects.

    RacGAP50C negatively regulates wg pathway activity during embryonic development.

    dsRNA directed against this gene causes defects in cytokinesis when tested in an RNAi screen in S2 cells.

    RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a binucleation phenotype when assayed in Kc167 cells.

    RacGAP50C alters the wg homozygous mutant cuticle pattern phenotype. The RacGAP50C mutation in a wild-type background reduces denticle diversity and produces a compression and lateral expansion of the denticle belts. In the first row of denticles in each belt, some or all of the denticles may be missing. RacGAP50C interacts genetically with the gene nkd, enhancing the nkd phenotype.

    dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.

    dsRNA made from templates generated with primers directed against this gene is tested in an RNAi screen for effects on actin-based lamella formation.

    Expression of RacGAP50C is repressed by Egfr/Ras signalling in the prospective wing veins and accumulates at the vein/intervein boundaries.

    RacGAP50C acts as a negative regulator of Rho-family GTPases Rac1 and Cdc42.

    Reduced RacGAP50C or increased Rac1 activity in the wing disc cause similar defects: widening of veins development of extra sensory organs, apoptosis and appearance of enlarged cells that differentiate multiple hairs with abnormal polarity.

    tum has a function in dendritic development.

    "49C--50D" was stated as revision.

    Origin and Etymology
    Discoverer
    Etymology

    The mutation was named "enhancer of naked" after the mutant phenotype.

    Identification
    External Crossreferences and Linkouts ( 39 )
    Sequence Crossreferences
    NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
    GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
    GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
    RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
    UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
    Other crossreferences
    Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
    Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
    Flygut - An atlas of the Drosophila adult midgut
    GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
    iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
    KEGG Genes - Molecular building blocks of life in the genomic space.
    modMine - A data warehouse for the modENCODE project
    Linkouts
    BioGRID - A database of protein and genetic interactions.
    DroID - A comprehensive database of gene and protein interactions.
    DRSC - Results frm RNAi screens
    Developmental Studies Hybridoma Bank - Monoclonal antibodies for use in research
    FLIGHT - Cell culture data for RNAi and other high-throughput technologies
    FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
    FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
    FlyMine - An integrated database for Drosophila genomics
    Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
    InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
    MIST (genetic) - An integrated Molecular Interaction Database
    MIST (protein-protein) - An integrated Molecular Interaction Database
    Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
    Synonyms and Secondary IDs (22)
    Reported As
    Symbol Synonym
    RhoGAP-50C14
    Secondary FlyBase IDs
    • FBgn0028559
    • FBgn0033881
    Datasets (0)
    Study focus (0)
    Experimental Role
    Project
    Project Type
    Title
    References (154)